FastQCFastQC Report
Fri 17 Jun 2016
SRR1526246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20477
Sequences flagged as poor quality0
Sequence length56
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1110.5420715925184353No Hit
TGTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG840.4102163402842213No Hit
TGTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG460.22464228158421642No Hit
TGTGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAAAAAAAAAAAAAAAAA440.21487522586316354No Hit
TGTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG390.19045758656053133No Hit
TGTGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAACAAAAAAAAAAAAAAA380.18557405870000487No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT310.15138936367631978No Hit
TGTGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG290.14162230795526687No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG280.13673878009474044No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.13673878009474044No Hit
TGTGCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG280.13673878009474044No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA280.13673878009474044No Hit
TGTGCGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG270.131855252234214No Hit
TGTGCGGGGAAGACGTCAGATAATCAAATTAAATAGACGTCTCCAACTTCTGGAAG250.12208819651316112No Hit
TGTGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACCAAAAAAAAAAAAAAA240.11720466865263465No Hit
TGTGCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAACCAAAAAAAAAAAAAAA240.11720466865263465No Hit
TGTGCGGGGACTGTATCAACGCAGAGTCGACTTTTTGTTTTTTTTTTTTTTTTTTT240.11720466865263465No Hit
TGTGCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAACAAAAAAAAAAAAAAA240.11720466865263465No Hit
TGTGCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA240.11720466865263465No Hit
TGTGCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA220.10743761293158177No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.10743761293158177No Hit
TGTGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA210.10255408507105533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTAGC150.00802592950.44554541
CCCACAG150.00802592950.44554540
ACTCACA150.00802592950.44554539
CCACAGT150.00802592950.44554541
ACCCACA150.00802592950.44554539
CTCACAG150.00802592950.44554540
GCGCCGG150.00810503650.32098846
CGCCGGA150.00810503650.32098847
TAGCGCC150.00818472150.19704444
TGTAGCG150.00818472150.19704442
GTAGCGC150.00818472150.19704443
GGGTACT150.00826498750.07370835
GGGTACC150.00826498750.07370835
GGTACCC150.00826498750.07370836
GAAGATG204.10708E-449.950989
AGAGTGG204.10708E-449.9509810
GGAATTC204.10708E-449.9509811
TTCGTGG150.00834583849.95097715
GAGTGCG150.00834583849.9509779
AAGAGTG150.00834583849.95097710
Produced by FastQC (version 0.11.5)