##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526246_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20477 Sequences flagged as poor quality 0 Sequence length 56 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.428334228646776 39.0 38.0 40.0 36.0 40.0 2 37.90359916003321 39.0 38.0 40.0 35.0 40.0 3 38.19333886799824 39.0 38.0 40.0 35.0 40.0 4 37.83234848854813 39.0 38.0 40.0 35.0 40.0 5 37.802851980270546 39.0 38.0 40.0 35.0 40.0 6 37.79977535771842 39.0 38.0 40.0 35.0 40.0 7 38.06973677784832 39.0 38.0 40.0 35.0 40.0 8 38.00146505835816 39.0 38.0 40.0 35.0 40.0 9 37.38819162963325 39.0 36.0 40.0 35.0 40.0 10 36.87341895785516 39.0 35.0 40.0 32.0 40.0 11 35.96615715192655 38.0 35.0 39.0 29.0 40.0 12 34.44562191727304 38.0 33.0 39.0 23.0 40.0 13 34.31137373638717 38.0 33.0 39.0 22.0 40.0 14 34.318943204570985 38.0 33.0 39.0 22.0 40.0 15 34.257899106314404 38.0 33.0 39.0 22.0 40.0 16 33.72994090931289 38.0 32.0 39.0 21.0 40.0 17 33.81877228109586 38.0 32.0 39.0 22.0 40.0 18 33.23328612589735 37.0 31.0 39.0 19.0 40.0 19 34.160375054939685 38.0 32.0 39.0 24.0 40.0 20 34.121453337891296 38.0 32.0 39.0 24.0 40.0 21 34.90438052449089 38.0 33.0 39.0 26.0 40.0 22 34.57410753528349 38.0 33.0 39.0 25.0 40.0 23 34.992577037652 38.0 34.0 39.0 26.0 40.0 24 34.63339356351028 38.0 33.0 39.0 25.0 40.0 25 34.91297553352542 38.0 33.0 39.0 26.0 40.0 26 35.45836792498901 38.0 35.0 39.0 28.0 40.0 27 35.47345802607804 38.0 35.0 39.0 28.0 40.0 28 35.42042291351272 38.0 35.0 39.0 28.0 40.0 29 35.137324803438005 38.0 35.0 39.0 27.0 40.0 30 34.99921863554231 38.0 35.0 39.0 27.0 40.0 31 35.45973531278996 38.0 35.0 39.0 29.0 40.0 32 35.23480001953411 38.0 35.0 39.0 27.0 40.0 33 35.39717732089662 38.0 35.0 39.0 29.0 40.0 34 35.38452898373785 38.0 35.0 39.0 29.0 40.0 35 35.273575230746694 38.0 35.0 39.0 28.0 40.0 36 34.54788299067246 38.0 34.0 39.0 28.0 40.0 37 34.27005909068711 38.0 33.0 39.0 27.0 40.0 38 33.77779948234605 37.0 33.0 39.0 25.0 40.0 39 33.40880011720467 37.0 32.0 39.0 23.0 40.0 40 30.242467158275137 35.0 26.0 39.0 9.0 40.0 41 26.670899057479122 32.0 15.0 38.0 5.0 39.0 42 31.11105142354837 35.0 30.0 38.0 11.0 40.0 43 29.734140743272942 35.0 27.0 38.0 5.0 39.0 44 29.682521853787176 35.0 27.0 38.0 4.0 39.0 45 30.70669531669678 35.0 29.0 38.0 4.0 40.0 46 30.64350246618157 35.0 29.0 38.0 4.0 40.0 47 30.091126629877422 35.0 28.0 38.0 4.0 40.0 48 30.175807002978953 35.0 28.0 38.0 4.0 40.0 49 27.66030180202178 35.0 17.0 38.0 4.0 40.0 50 29.80529374420081 35.0 27.0 38.0 4.0 40.0 51 29.951555403623576 35.0 28.0 39.0 4.0 40.0 52 29.72251794696489 35.0 27.0 39.0 4.0 40.0 53 29.341163256336376 35.0 27.0 39.0 4.0 40.0 54 28.22244469404698 35.0 19.0 39.0 4.0 40.0 55 28.645407042047175 35.0 24.0 39.0 4.0 40.0 56 27.643453630902965 35.0 17.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.07412383263577738 1 2 0.11616341257087015 1 3 0.05574661765861322 1 4 0.3805407849167963 1 5 0.5674202248372069 1 6 0.35475013057158833 1 7 0.32617596460396214 1 8 0.1982668655316857 1 9 0.3304607866295797 1 10 0.47675161596917803 1 11 -3.679764738883545 1 12 -13.471577360500014 1 13 -8.124641461297013 1 14 -5.969816991255762 1 15 -8.606174062996974 1 16 -23.650883612525433 1 17 -5.2986548002416285 1 18 -12.533827279573902 1 19 -7.535920200956053 1 20 -7.5280651095280575 1 21 -8.100541095438128 1 22 -4.8580447833649 1 23 -4.7769810902354095 1 24 -5.399865998408696 1 25 -4.689394555284995 1 26 1.280857480152136 1 27 1.471284114253649 1 28 0.9633191323679142 1 29 1.7533070662474444 1 30 1.1100270838351918 1 31 1.3904330296573093 1 32 1.0192711629272537 1 33 1.54193007904977 1 34 1.2948756928145073 1 35 -0.3658573919513941 1 36 0.3311868051714626 1 37 1.1071238375566068 1 38 1.1781796937209208 1 39 2.2212602665988044 1 40 4.0897963631603 1 41 -7.921680900923416 1 42 -8.254951490810264 1 43 -15.76985587160379 1 44 -22.889446392159428 1 45 -9.709610501418787 1 46 -14.763088348067628 1 47 -22.871810068540558 1 48 -12.038344827568014 1 49 -19.75112797450567 1 50 -11.297931386263851 1 51 -7.470181978758514 1 52 -8.083397903819847 1 53 -7.663174638503179 1 54 -6.8060200343834225 1 55 -7.64278479106364 1 56 -1.9346317631459904 2 1 0.3265171930052517 2 2 0.01359931000676795 2 3 -0.4602790233670291 2 4 0.6081048874808985 2 5 0.05139458381156459 2 6 0.7425706433920993 2 7 0.34540673383472864 2 8 0.7367284039932258 2 9 0.17981976098855057 2 10 0.8229054621230247 2 11 0.9612609021420937 2 12 -3.1735004374230904 2 13 -0.6022055638611121 2 14 3.078259931821158 2 15 3.9963900395671246 2 16 3.176039464397647 2 17 2.7878836612968314 2 18 -1.1235708693174935 2 19 2.6243362093003597 2 20 2.6450118135488623 2 21 2.671894801997773 2 22 2.4624680371479215 2 23 1.492249678995357 2 24 1.5680827195400227 2 25 2.317015701125257 2 26 1.8898318391264937 2 27 2.0353866783562182 2 28 1.7069088759576587 2 29 2.2789480918884735 2 30 0.33438605819416267 2 31 2.2429971322214115 2 32 1.9647839834400713 2 33 0.9329557200754124 2 34 2.3589782569170765 2 35 1.9129887618947592 2 36 3.110032959017616 2 37 2.132764863197636 2 38 3.3768976424388697 2 39 4.823824369162907 2 40 5.250052773416716 2 41 -2.421680900923416 2 42 -5.9344386702974425 2 43 -11.167291769039686 2 44 -14.94072844344148 2 45 -3.898713065521349 2 46 -8.298344758324038 2 47 -16.96155365828415 2 48 -5.221037135260321 2 49 -14.026769000146695 2 50 -5.278700617033081 2 51 -0.33556659414313117 2 52 -0.23403892946087268 2 53 0.038748438419897724 2 54 0.706800478437092 2 55 0.8475998243209766 2 56 4.632675929161703 3 1 0.13420950069755833 3 2 0.6890800792375344 3 3 0.22241328432527752 3 4 0.46387411825013203 3 5 0.10908689150387119 3 6 0.4589167972382526 3 7 0.5136759646039621 3 8 -0.7079831344683143 3 9 0.840877453296244 3 10 0.883001615969178 3 11 2.341068594449787 3 12 4.059672639499986 3 13 2.6670252053696544 3 14 4.686433008744238 3 15 3.258409270336358 3 16 3.9741163874745666 3 17 4.3888451997583715 3 18 4.70575605375943 3 19 2.7974131323772795 3 20 3.5656848904719425 3 21 3.3161255712285396 3 22 3.746121883301768 3 23 2.2855189097645905 3 24 3.308467334924636 3 25 2.237688778048337 3 26 1.1350241468188003 3 27 1.6171174475869847 3 28 0.9737357990345785 3 29 1.86789039958078 3 30 0.9641937505018561 3 31 0.848766362990645 3 32 1.810937829593918 3 33 0.5627634123831058 3 34 0.19070902614784302 3 35 1.634142608048606 3 36 1.0499368051714626 3 37 1.9717071708899425 3 38 1.4802630270542565 3 39 3.17959359993214 3 40 6.8606296964936355 3 41 6.953319099076584 3 42 2.692965175856404 3 43 -2.874022538270456 3 44 -0.6811130588260959 3 45 2.363306165247881 3 46 1.4973283185990383 3 47 -2.225976735207226 3 48 1.4616551724319855 3 49 1.5092886921609967 3 50 0.2333186137361487 3 51 0.6548180212414856 3 52 1.5520187628468207 3 53 1.524325361496821 3 54 1.9856466322832453 3 55 2.57596520893636 3 56 3.0653682368540096 4 1 0.5652439834561775 4 2 -0.37234233455556875 4 3 -0.18706947429541287 4 4 0.33887411825013203 4 5 -0.7788441429788904 4 6 -0.36220389241692175 4 7 -0.06175506987879942 4 8 0.2327496241523761 4 9 0.6652309015721087 4 10 0.6836481676933133 4 11 2.2742582496222 4 12 4.12756057053447 4 13 3.132542446748964 4 14 3.3103554225373415 4 15 3.2444006496466997 4 16 3.478426732302154 4 17 4.119448648034236 4 18 4.420195708931843 4 19 3.4784476151358987 4 20 3.308141787023665 4 21 2.354918674676817 4 22 2.8948287798534906 4 23 1.9988809787301065 4 24 1.7696742314763583 4 25 3.1008353297724724 4 26 2.1975241468188003 4 27 2.3735829648283655 4 28 1.6817099369656106 4 29 1.6556059168221609 4 30 2.078417888432888 4 31 1.5944560181630578 4 32 1.2764550709732276 4 33 0.8149185847968994 4 34 0.6109676468375014 4 35 1.2936253666692963 4 36 2.611359218964566 4 37 2.750801998476149 4 38 3.0373750960197725 4 39 4.451145324070069 4 40 6.4489917654591515 4 41 10.31969840942141 4 42 5.049646210339166 4 43 8.666925737591615 4 44 7.823197286001491 4 45 5.952745820420294 4 46 6.623405904805935 4 47 5.961523264792774 4 48 7.121137931052676 4 49 10.28622834733341 4 50 5.383103096494768 4 51 2.934990435034589 4 52 4.009992900777853 4 53 3.961825361496821 4 54 5.274439735731523 4 55 4.870146243419121 4 56 3.9748509954747 5 1 0.3761449845685263 5 2 0.6054107243988227 5 3 0.13773586497043766 5 4 0.33887411825013203 5 5 -0.060267947205808525 5 6 0.8480297004640605 5 7 0.6568211258942824 5 8 0.8756862203703975 5 9 1.3237403565220518 5 10 0.9283645191949859 5 11 3.0406653686433387 5 12 4.532454897564506 5 13 4.134767140853526 5 14 4.04227978293779 5 15 4.56586894775571 5 16 4.8087938068294065 5 17 5.423119393306756 5 18 5.154344763436846 5 19 4.676445390441799 5 20 4.2380639227300065 5 21 3.779835248647892 5 22 3.953783173624352 5 23 3.626244716216206 5 24 4.667338302666572 5 25 2.861680713532209 5 26 2.7136531790768643 5 27 2.9820368024256965 5 28 1.5815987022603863 5 29 2.360833947967876 5 30 2.1395969763083045 5 31 1.8124760404099973 5 32 1.536744281206822 5 33 1.2220376059314901 5 34 0.5153058003413946 5 35 1.77527164030667 5 36 1.5045739019456548 5 37 2.8920700741157503 5 38 2.342158188344577 5 39 1.812658116061172 5 40 6.334420019074283 5 41 8.465415873270135 5 42 3.911715175856404 5 43 4.724767784310192 5 44 4.443886941173904 5 45 2.848185197505945 5 46 2.8269654153732304 5 47 3.638942619631486 5 48 2.7963325917868254 5 49 5.923603208290029 5 50 2.871423452445825 5 51 2.3443341502737454 5 52 0.39375263381456094 5 53 0.6392447163355293 5 54 1.3445176000251813 5 55 1.6031829508718438 5 56 3.2629488820153014 6 1 0.6342095006975583 6 2 1.090522386929841 6 3 0.3858748227868176 6 4 0.41579719517321223 6 5 -0.2947592623422821 6 6 0.3964167972382526 6 7 0.9223298107578088 6 8 0.4290360963009192 6 9 0.9490505302193242 6 10 -0.523248384030822 6 11 2.192030132911327 6 12 3.6726534087307527 6 13 3.8593328976773478 6 14 3.039798393359625 6 15 3.8810054241825114 6 16 5.676039464397647 6 17 5.441729815142985 6 18 5.33796759222097 6 19 4.5474131323772795 6 20 3.2603964289334826 6 21 3.210356340459306 6 22 3.154775729455615 6 23 2.876865063610744 6 24 3.337313488770789 6 25 3.0477849318944905 6 26 2.3898318391264937 6 27 0.6892328322023715 6 28 2.860755029803812 6 29 2.5097173226577 6 30 3.2574629812710896 6 31 2.6660740552983384 6 32 1.5417070603631515 6 33 3.240648027767719 6 34 0.7435936415324562 6 35 1.1822195311255257 6 36 1.8792637282483824 6 37 2.9019956324284024 6 38 3.45382071936195 6 39 3.7084397537782934 6 40 7.019283542647482 6 41 5.001396022153507 6 42 6.219407483548711 6 43 8.101939000191084 6 44 7.251579248866211 6 45 5.601286934478647 6 46 5.4324244724451916 6 47 6.192292495562008 6 48 5.625116710893526 6 49 6.742461769084077 6 50 4.913607075274609 6 51 3.933664175087639 6 52 2.881345685923744 6 53 3.3079792076506678 6 54 2.8221850938217052 6 55 2.655292132013283 6 56 4.632675929161703 7 1 1.0984952149832736 7 2 1.8542586506661038 7 3 1.0616989986109928 7 4 1.7674455468215626 7 5 1.3590868915038712 7 6 1.6821310829525373 7 7 1.3976045360325315 7 8 1.662552579817401 7 9 2.0864131675819593 7 10 2.1910373302548933 7 11 3.4749971658783565 7 12 5.439136925214271 7 13 5.095596633941085 7 14 5.333754437315669 7 15 5.655730698907789 7 16 5.956259244617421 7 17 6.219202342615517 7 18 7.013791768045145 7 19 5.190270275234425 7 20 5.829077747614797 7 21 4.44112557122854 7 22 4.415764740444622 7 23 4.437304624050306 7 24 4.683467334924636 7 25 4.8582244923340525 7 26 4.126095575390231 7 27 4.126046019015554 7 28 3.7728429418917244 7 29 4.443783256723634 7 30 4.705265179073287 7 31 3.9380520772763603 7 32 4.025223543879633 7 33 3.446691983811675 7 34 3.771066169004989 7 35 3.901999750905752 7 36 4.384758233742893 7 37 5.333314313747088 7 38 4.868655884197111 7 39 5.768879314217855 7 40 8.851701125065066 7 41 3.1854619562194415 7 42 6.661715175856404 7 43 7.563477461729544 7 44 7.443886941173904 7 45 6.1177704509621655 7 46 6.457149747170469 7 47 6.711523264792774 7 48 7.229512315289131 7 49 9.701252977875281 7 50 8.166354328021864 7 51 8.601246592670055 7 52 8.065411619989675 7 53 8.426111075782536 7 54 6.932075203711815 7 55 6.66078663750779 7 56 6.511796808282579 8 1 0.26383913032718453 8 2 1.097644894052351 8 3 0.7733392102512013 8 4 1.0425778219538344 8 5 0.8405683729853521 8 6 0.7297501305715883 8 7 0.7798796683076645 8 8 1.2353039025687238 8 9 0.872127453296244 8 10 0.6619368011543614 8 11 3.060976001857192 8 12 5.181200417277765 8 13 4.907765946110395 8 14 5.136664490225719 8 15 4.319751862928953 8 16 4.988005276363452 8 17 5.770789644202814 8 18 5.206913461166835 8 19 4.28815387311802 8 20 3.7589719275089806 8 21 3.4226070527100205 8 22 4.086399661079547 8 23 2.056352243097926 8 24 3.8501340015913 8 25 4.33838322249278 8 26 3.604931554226205 8 27 3.7860989290684657 8 28 2.0559117249605023 8 29 3.2162700292104063 8 30 3.2674344912425966 8 31 2.936729325953607 8 32 3.755382274038361 8 33 2.23637452349422 8 34 1.8319127298515454 8 35 2.5184018673078654 8 36 3.622853471838127 8 37 3.4404571708899425 8 38 3.057809323350554 8 39 5.2582973036358425 8 40 5.987944511308452 8 41 -0.03279201203452686 8 42 3.6524559165971446 8 43 5.489403387655468 8 44 6.258701755988721 8 45 3.443167276358995 8 46 3.093393133413855 8 47 3.1837454870149955 8 48 3.75332183909865 8 49 6.406279432901737 8 50 3.7854019470694844 8 51 4.13166987309334 8 52 3.9397502443283017 8 53 4.887751287422745 8 54 3.453239224875837 8 55 3.435918912640062 8 56 5.236664533150307 9 1 0.6342095006975583 9 2 0.7161634125708645 9 3 0.4140799509919475 9 4 1.3055407849168006 9 5 1.3590868915038712 9 6 1.0297501305715855 9 7 1.0095092979372922 9 8 0.23160019886501715 9 9 1.6387941199629097 9 10 1.9434182826358466 9 11 3.1202352611164557 9 12 4.436755972833318 9 13 4.300358538702987 9 14 4.988516342077574 9 15 4.8271592703363595 9 16 4.032449720807897 9 17 5.3930118664250415 9 18 5.666172720426097 9 19 3.4140797990439467 9 20 4.255268223805274 9 21 3.6411255712285424 9 22 4.275288549968437 9 23 3.556352243097926 9 24 4.41680066825797 9 25 3.793938778048336 9 26 3.5641908134854674 9 27 2.8046174475869847 9 28 2.8299857990345814 9 29 3.6199737329141115 9 30 3.160027083835189 9 31 1.2404330296573107 9 32 2.9109378295939194 9 33 1.4585967457164415 9 34 2.561542359481173 9 35 2.5924759413819416 9 36 1.2228534718381283 9 37 1.3071238375566097 9 38 1.2615130270542565 9 39 1.5879269332654715 9 40 3.8731296964936384 9 41 2.578319099076584 9 42 2.911715175856404 9 43 5.096810795062876 9 44 5.27722027450724 9 45 3.1653894985812165 9 46 4.845244985265708 9 47 5.194856598126108 9 48 5.5199885057653155 9 49 8.698872025494328 9 50 6.085401947069482 9 51 4.479818021241488 9 52 4.324935429513488 9 53 4.228492028163487 9 54 4.427313298949912 9 55 4.698881875603025 9 56 5.5737015701873425 10 1 0.018824885312945128 10 2 0.24436854077599435 10 3 -0.421817484905489 10 4 -0.12266434328832787 10 5 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-1.8268703035063645 120 41 -2.0050142342567483 120 42 -5.088284824143596 120 43 -10.853189204937124 120 44 -9.556113058826096 120 45 -6.834610501418787 120 46 -14.388088348067628 120 47 -14.538476735207226 120 48 -9.496678160901347 120 49 -18.917794641172335 120 50 -10.881264719597183 120 51 -9.803515312091847 120 52 -9.208397903819847 120 53 -10.038174638503179 120 54 -9.389353367716755 120 55 -8.26778479106364 120 56 -4.476298429812658 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 37.0 5 5.0 6 22.0 7 16.0 8 36.0 9 72.0 10 32.0 11 35.0 12 21.0 13 21.0 14 31.0 15 37.0 16 53.0 17 70.0 18 87.0 19 108.0 20 153.0 21 174.0 22 234.0 23 281.0 24 325.0 25 354.0 26 349.0 27 464.0 28 517.0 29 584.0 30 771.0 31 913.0 32 997.0 33 1204.0 34 1384.0 35 1672.0 36 2018.0 37 2838.0 38 2727.0 39 1831.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.45792840748156 0.42486692386580066 0.087903501489476 0.029301167163158663 8 98.31029936025784 1.2404160765737169 0.18557405870000487 0.263710504468428 9 91.95682961371294 5.117937197831714 1.635981833276359 1.2892513551789813 10 28.534453289056017 52.67373150363823 9.986814474776578 8.80500073252918 11 25.3552766518533 32.52429555110612 23.455584314108513 18.66484348293207 12 39.86912145333789 21.795184841529522 17.99091663817942 20.344777066953167 13 21.443923407581085 23.09007424775303 21.88843298163345 33.57756936303243 14 30.69785613126923 24.383454607608538 22.937930360892707 21.980758900229524 15 26.42479435957697 24.500587544065805 21.63141402271837 27.443204073638856 16 23.269334115003176 25.687625189310666 31.261908251502273 19.78113244418389 17 34.25794794159301 24.06602529667432 19.988279533134737 21.68774722859794 18 31.51828881183767 25.46271426478488 19.665966694339993 23.353030229037454 19 25.788161689232126 32.435602913143356 20.988318099613863 20.787917298010655 20 21.796939922764825 30.160825145426994 22.090238060321653 25.951996871486532 21 24.383454607608538 22.322605850466378 30.76134199345607 22.532597548469013 22 28.14865458807442 25.042730868779607 24.232065243932215 22.576549299213752 23 25.252722566782243 35.4934804903062 20.486399374908434 18.767397568003126 24 25.970601162279632 32.050593348635054 21.380084973384772 20.598720515700542 25 25.242955511061187 34.84397128485618 21.785417785808466 18.12765541827416 26 24.876666829482733 26.913495823767892 31.592829580423 16.61700776632638 27 31.963448006254886 22.63487099296325 27.731626270523847 17.670054730258013 28 24.954783203793323 28.938749572273547 30.06305909957472 16.04340812435841 29 21.24780144615986 30.159273011530193 31.571233144420557 17.02169239788939 30 17.35052754982415 26.118601016021888 38.813989839781165 17.7168815943728 31 14.356895119925749 29.37814469249182 41.888525230814324 14.376434956768112 32 18.98127780221929 26.729236936012125 39.36061006012611 14.92887520164247 33 15.529043614316448 31.894191277136713 41.071777821239976 11.504987287306864 34 12.942153605628299 27.437951924956028 43.81473519640414 15.80515927301153 35 18.060102614219396 27.24163205472758 44.22672856095773 10.471536770095284 36 18.062341215555993 26.978698456126637 43.77076411960133 11.188196208716045 37 18.45476772616137 26.62591687041565 44.06356968215159 10.855745721271393 38 15.264111225339011 26.998580310373526 47.843540412199545 9.893768052087923 39 12.964774951076322 31.795499021526417 43.806262230919764 11.433463796477495 40 9.769635050837469 21.55803330222506 40.45385333267843 28.21847831425905 41 12.548595049456226 19.11815363417155 37.79341567836229 30.539835638009937 42 12.440074356716565 28.441444085705896 44.84884062224832 14.269640935329225 43 10.64839735747492 27.981404453144116 43.03401027648642 18.336187912894545 44 10.760858907736052 26.394744582802236 41.45504461221689 21.389351897244826 45 12.247993736543355 32.84889410843609 44.32374241534547 10.579369739675084 46 16.154598825831705 27.954990215264186 44.84344422700587 11.04696673189824 47 12.410221331900132 27.957199394146677 47.3249621341672 12.307617139785997 48 11.855039859148041 28.982246784369348 49.17102753460165 9.99168582188096 49 9.294683146949186 24.298607023739454 40.42574063174416 25.980969197567195 50 10.713936430317847 29.911980440097803 48.220048899755504 11.15403422982885 51 16.202320912696468 31.014052783626305 43.86231209910395 8.921314204573275 52 12.050843314593008 35.04766560743094 42.5861647518944 10.315326326081644 53 14.71422979056567 32.48678802113917 43.986102955568605 8.812879232726562 54 10.12478590653291 30.878394910692442 44.06165891852215 14.93516026425251 55 11.502692119432208 32.22222222222222 42.81448849730788 13.46059716103769 56 16.38365549302667 31.808172253486667 41.908490335209194 9.899681918277466 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 3.5 9 6.0 10 15.5 11 25.0 12 51.0 13 109.5 14 142.0 15 151.0 16 160.0 17 164.5 18 169.0 19 199.0 20 229.0 21 262.0 22 351.5 23 408.0 24 454.5 25 501.0 26 638.5 27 776.0 28 962.5 29 1257.0 30 1365.0 31 1395.0 32 1425.0 33 1406.5 34 1388.0 35 1246.5 36 1105.0 37 963.0 38 692.5 39 564.0 40 473.5 41 383.0 42 381.0 43 379.0 44 378.0 45 377.0 46 374.5 47 423.5 48 475.0 49 596.5 50 718.0 51 779.5 52 841.0 53 755.0 54 705.0 55 741.0 56 725.5 57 710.0 58 721.5 59 733.0 60 723.0 61 713.0 62 692.0 63 617.0 64 563.0 65 497.5 66 432.0 67 384.5 68 337.0 69 308.5 70 280.0 71 234.0 72 164.0 73 140.0 74 101.0 75 62.0 76 56.5 77 51.0 78 36.0 79 14.0 80 7.0 81 6.0 82 5.0 83 3.5 84 2.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.024417639302632223 14 0.0 15 0.25882697660790155 16 0.03906822288421156 17 0.0 18 0.0 19 0.087903501489476 20 0.09767055721052889 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.01953411144210578 27 0.06348586218684378 28 0.09767055721052889 29 0.043951750744738 30 0.024417639302632223 31 0.029301167163158663 32 0.09767055721052889 33 0.12208819651316112 34 0.043951750744738 35 0.058602334326317326 36 0.043951750744738 37 0.131855252234214 38 0.24417639302632224 39 0.18069053083947845 40 0.5762562875421204 41 0.7618303462421253 42 0.17092347511842554 43 0.20510817014211066 44 0.3857987009815891 45 0.20022464228158424 46 0.18069053083947845 47 0.048835278605264446 48 0.14650583581579332 49 0.43463397958685357 50 0.131855252234214 51 0.263710504468428 52 0.10743761293158177 53 0.20022464228158424 54 0.20510817014211066 55 0.22952580944474288 56 0.20510817014211066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 20477.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.8764955804073 #Duplication Level Percentage of deduplicated Percentage of total 1 87.72074704611866 67.43663622600967 2 7.203659001397535 11.075841187673975 3 2.2360564096048785 5.157005420715925 4 1.067208740947783 3.2817307222737706 5 0.5844238343285478 2.2464228158421644 6 0.23504002032778554 1.0841431850368706 7 0.2096302884004574 1.1280949357816086 8 0.19057298945496123 1.1720466865263466 9 0.09528649472748062 0.65927626117107 >10 0.4446703087282429 5.806514626165942 >50 0.006352432981832041 0.4102163402842213 >100 0.006352432981832041 0.5420715925184353 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 111 0.5420715925184353 No Hit TGTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 84 0.4102163402842213 No Hit TGTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 46 0.22464228158421642 No Hit TGTGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.21487522586316354 No Hit TGTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 39 0.19045758656053133 No Hit TGTGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAACAAAAAAAAAAAAAAA 38 0.18557405870000487 No Hit TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 31 0.15138936367631978 No Hit TGTGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 29 0.14162230795526687 No Hit TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 28 0.13673878009474044 No Hit TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28 0.13673878009474044 No Hit TGTGCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG 28 0.13673878009474044 No Hit TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 28 0.13673878009474044 No Hit TGTGCGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 27 0.131855252234214 No Hit TGTGCGGGGAAGACGTCAGATAATCAAATTAAATAGACGTCTCCAACTTCTGGAAG 25 0.12208819651316112 No Hit TGTGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACCAAAAAAAAAAAAAAA 24 0.11720466865263465 No Hit TGTGCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAACCAAAAAAAAAAAAAAA 24 0.11720466865263465 No Hit TGTGCGGGGACTGTATCAACGCAGAGTCGACTTTTTGTTTTTTTTTTTTTTTTTTT 24 0.11720466865263465 No Hit TGTGCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAACAAAAAAAAAAAAAAA 24 0.11720466865263465 No Hit TGTGCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA 24 0.11720466865263465 No Hit TGTGCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.10743761293158177 No Hit TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22 0.10743761293158177 No Hit TGTGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 21 0.10255408507105533 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.00976705572105289 0.0 0.0 0.0 0.0 11 0.01953411144210578 0.0 0.0 0.0 0.0 12 0.024417639302632223 0.0 0.0 0.0 0.0 13 0.03418469502368511 0.0 0.0 0.0 0.0 14 0.043951750744738 0.0 0.0 0.0 0.0 15 0.053718806465790886 0.0 0.0 0.0 0.0 16 0.053718806465790886 0.0 0.0 0.0 0.0 17 0.07325291790789666 0.0 0.0 0.0 0.0 18 0.087903501489476 0.0 0.0 0.0 0.0 19 0.10255408507105533 0.0 0.0 0.0 0.0 20 0.10255408507105533 0.0 0.0 0.0 0.0 21 0.10743761293158177 0.0 0.0 0.0 0.0 22 0.11720466865263467 0.0 0.0 0.0 0.0 23 0.18557405870000487 0.0 0.0 0.0 0.0 24 0.2002246422815842 0.0 0.0 0.0 0.0 25 0.21487522586316354 0.0 0.0 0.0 0.0 26 0.23929286516579576 0.0 0.0 0.0 0.0 27 0.2539434487473751 0.0 0.0 0.0 0.0 28 0.2783610880500073 0.0 0.0 0.0 0.0 29 0.2978951994921131 0.0 0.0 0.0 0.0 30 0.31254578307369246 0.0 0.0 0.0 0.0 31 0.31254578307369246 0.0 0.0 0.0 0.0 32 0.3223128387947453 0.0 0.0 0.0 0.0 33 0.32719636665527174 0.0 0.0 0.0 0.0 34 0.3320798945157982 0.0 0.0 0.0 0.0 35 0.3320798945157982 0.0 0.0 0.0 0.0 36 0.3320798945157982 0.0 0.0 0.0 0.0 37 0.3320798945157982 0.0 0.0 0.0 0.0 38 0.33696342237632465 0.0 0.0 0.0 0.0 39 0.3418469502368511 0.0 0.0 0.0 0.0 40 0.34673047809737756 0.0 0.0 0.0 0.0 41 0.351614005957904 0.0 0.0 0.0 0.0 42 0.3662645895394833 0.0 0.0 0.0 0.0 43 0.37603164526053623 0.0 0.0 0.0 0.0 44 0.38091517312106266 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTAGC 15 0.008025929 50.445545 41 CCCACAG 15 0.008025929 50.445545 40 ACTCACA 15 0.008025929 50.445545 39 CCACAGT 15 0.008025929 50.445545 41 ACCCACA 15 0.008025929 50.445545 39 CTCACAG 15 0.008025929 50.445545 40 GCGCCGG 15 0.008105036 50.320988 46 CGCCGGA 15 0.008105036 50.320988 47 TAGCGCC 15 0.008184721 50.197044 44 TGTAGCG 15 0.008184721 50.197044 42 GTAGCGC 15 0.008184721 50.197044 43 GGGTACT 15 0.008264987 50.073708 35 GGGTACC 15 0.008264987 50.073708 35 GGTACCC 15 0.008264987 50.073708 36 GAAGATG 20 4.10708E-4 49.95098 9 AGAGTGG 20 4.10708E-4 49.95098 10 GGAATTC 20 4.10708E-4 49.95098 11 TTCGTGG 15 0.008345838 49.950977 15 GAGTGCG 15 0.008345838 49.950977 9 AAGAGTG 15 0.008345838 49.950977 10 >>END_MODULE