FastQCFastQC Report
Fri 17 Jun 2016
SRR1526245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43300
Sequences flagged as poor quality0
Sequence length56
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGGTGCTGCTGAGCACCTTTCTGGGATGTTGTCCTGGCTGTTGCCAAAA2600.6004618937644343No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA2330.5381062355658199No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.4965357967667437No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA1340.3094688221709007No Hit
GCTGTGGGGTGCTGCTGAGCACCTTTCTGGGATGTTGTCCTGGCTGTTGCCAAAAA1180.27251732101616627No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA1030.23787528868360278No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.2286374133949192No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA970.22401847575057734No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT780.18013856812933027No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA620.14318706697459582No Hit
GCTGTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.12933025404157045No Hit
GCTGTGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.11778290993071593No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAACAAAAAAAAAAAAAAA480.11085450346420324No Hit
GCTGTGGGGAACTATATAGTAATCATTAGTAGACTGTTGCCTTGTGGGGTTCCTGT480.11085450346420324No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA460.10623556581986143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAAAC650.050.4624443
TGGCTCG204.1178614E-450.05122431
TTCGTGG150.00841953649.93496315
GAAATGG204.1654913E-449.9349627
AAATGGC204.1654913E-449.9349628
CAGTTTT150.00845831649.87703326
AGAAATG204.1894696E-449.8770326
TCGTGGA150.0084972349.81923716
CGTGGAG204.2135577E-449.81923717
ACTGTAC150.00853627649.76157810
GAAGCCG301.0587519E-649.7615789
CGCAGTT150.00853627649.76157824
GACTGTA150.00853627649.7615789
GACTGGT150.00853627649.7615789
GAGAGTG301.0587519E-649.7615789
AAGTGTC150.00853627649.76157810
AAGCCTG150.00853627649.76157810
GGTGCAA150.00853627649.7615789
GAAGTGT252.1128782E-549.7615749
GAACCCC402.6921043E-949.7615749
Produced by FastQC (version 0.11.5)