##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526245_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43300 Sequences flagged as poor quality 0 Sequence length 56 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.018568129330255 39.0 38.0 40.0 35.0 40.0 2 37.61983833718245 39.0 38.0 39.0 35.0 40.0 3 37.57937644341801 39.0 38.0 39.0 35.0 40.0 4 37.36653579676674 39.0 38.0 39.0 34.0 40.0 5 37.178036951501156 39.0 36.0 39.0 33.0 40.0 6 37.56027713625866 39.0 38.0 39.0 35.0 40.0 7 37.72958429561201 39.0 38.0 40.0 35.0 40.0 8 37.65357967667436 39.0 38.0 40.0 35.0 40.0 9 36.91990762124711 39.0 35.0 40.0 32.0 40.0 10 36.56461893764434 39.0 35.0 39.0 32.0 40.0 11 35.507898383371824 38.0 35.0 39.0 28.0 40.0 12 34.20829099307159 38.0 33.0 39.0 22.0 40.0 13 34.02390300230947 38.0 33.0 39.0 22.0 40.0 14 34.19644341801386 38.0 33.0 39.0 22.0 40.0 15 34.08025404157044 38.0 33.0 39.0 21.0 40.0 16 33.48618937644342 38.0 31.0 39.0 20.0 40.0 17 33.38117782909931 37.0 31.0 39.0 20.0 40.0 18 32.84660508083141 37.0 30.0 39.0 17.0 40.0 19 33.77314087759815 37.0 31.0 39.0 23.0 40.0 20 33.9970207852194 38.0 32.0 39.0 23.0 40.0 21 34.486120092378755 38.0 33.0 39.0 24.0 40.0 22 34.03540415704388 38.0 33.0 39.0 22.0 40.0 23 34.666027713625866 38.0 33.0 39.0 25.0 40.0 24 34.268614318706696 38.0 33.0 39.0 23.0 40.0 25 34.589907621247114 38.0 33.0 39.0 25.0 40.0 26 35.085496535796764 38.0 35.0 39.0 27.0 40.0 27 34.993163972286375 38.0 35.0 39.0 27.0 40.0 28 35.03584295612009 38.0 35.0 39.0 27.0 40.0 29 34.652078521939956 38.0 34.0 39.0 25.0 40.0 30 34.66558891454965 38.0 34.0 39.0 25.0 40.0 31 34.850854503464205 38.0 35.0 39.0 26.0 40.0 32 34.5480831408776 38.0 34.0 39.0 24.0 40.0 33 34.65775981524249 38.0 35.0 39.0 26.0 40.0 34 34.74443418013857 38.0 35.0 39.0 27.0 40.0 35 34.50935334872979 38.0 35.0 39.0 25.0 40.0 36 33.77824480369515 37.0 33.0 39.0 25.0 40.0 37 33.5238337182448 37.0 33.0 39.0 23.0 40.0 38 33.198799076212474 37.0 32.0 39.0 21.0 40.0 39 32.63076212471132 36.0 32.0 39.0 17.0 40.0 40 29.70064665127021 35.0 25.0 38.0 6.0 39.0 41 26.371801385681295 32.0 14.0 38.0 4.0 39.0 42 30.287344110854505 35.0 28.0 38.0 4.0 39.0 43 28.99812933025404 34.0 25.0 38.0 4.0 39.0 44 28.906882217090068 34.0 25.0 38.0 4.0 39.0 45 29.91757505773672 35.0 28.0 38.0 4.0 39.0 46 29.581408775981526 35.0 27.0 38.0 4.0 40.0 47 29.069792147806005 35.0 25.0 38.0 4.0 40.0 48 29.053533487297923 35.0 25.0 38.0 4.0 40.0 49 26.658706697459586 33.0 13.0 38.0 4.0 39.0 50 28.560785219399538 35.0 24.0 38.0 4.0 40.0 51 28.693140877598154 35.0 24.0 39.0 4.0 40.0 52 28.489376443418013 35.0 23.0 39.0 4.0 40.0 53 28.100207852193996 35.0 21.0 39.0 4.0 40.0 54 27.183487297921477 35.0 13.0 39.0 4.0 40.0 55 27.42295612009238 35.0 17.0 39.0 4.0 40.0 56 26.567644341801387 34.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.32788789347947755 1 2 0.18461229860014328 1 3 0.8368281532993365 1 4 0.4632323114266441 1 5 0.06038965912728145 1 6 0.36982980757466777 1 7 0.4841334464342424 1 8 0.6870296211630418 1 9 0.5758874792287401 1 10 0.2888691843629019 1 11 -3.471515524753631 1 12 -11.813431235179031 1 13 -6.761902505609321 1 14 -5.493856442106047 1 15 -5.8294345686434355 1 16 -22.969981935253543 1 17 -4.251109311511339 1 18 -11.19193161897315 1 19 -4.973049290718155 1 20 -5.24111727863653 1 21 -5.6245793724149 1 22 -3.4522287073359124 1 23 -3.630682473258343 1 24 -4.201419484936562 1 25 -2.9358343473198545 1 26 1.0947500445038756 1 27 2.455032796951336 1 28 2.0760396363109734 1 29 2.352416738914151 1 30 2.076410884778852 1 31 0.9409960560706523 1 32 1.8940474908576732 1 33 0.6191159008395317 1 34 -0.1460739104250024 1 35 0.54776818380941 1 36 1.6434437805252387 1 37 2.543281688762846 1 38 2.5982203308839473 1 39 1.3440295628741268 1 40 2.2566083020686456 1 41 -13.162199013296911 1 42 -12.223765670630051 1 43 -19.35527055630242 1 44 -21.53745042819201 1 45 -12.92068838864052 1 46 -17.202737458277984 1 47 -22.487793356937566 1 48 -14.988520199790957 1 49 -19.305180280575698 1 50 -14.235216641784152 1 51 -10.636821632067438 1 52 -10.312770129783548 1 53 -10.883060341144716 1 54 -10.380056780763947 1 55 -10.032358395438422 1 56 -5.727141330641366 2 1 0.3996466687325082 2 2 0.352815063577097 2 3 1.1357912869398916 2 4 0.7621954450671993 2 5 0.35244035037152344 2 6 0.8629173651783546 2 7 0.9564836768489897 2 8 0.6795411419003727 2 9 0.8212791842978646 2 10 0.6863346221509161 2 11 -0.014142252864232319 2 12 -6.61527455315138 2 13 -3.1086766991577086 2 14 1.9721573827787466 2 15 1.6953350166100236 2 16 1.7529443320275675 2 17 2.55419022765917 2 18 -1.7305260890192322 2 19 2.0695774373924465 2 20 2.376394242100801 2 21 1.2498445907187374 2 22 2.9314118456594827 2 23 2.4200087709812905 2 24 1.530147335339933 2 25 1.685133394615626 2 26 1.8430219339047937 2 27 1.7960466218361262 2 28 1.4049566870022119 2 29 1.8311033748588486 2 30 0.877102129018482 2 31 0.2676089592964601 2 32 1.0023424217332462 2 33 0.5119730436966776 2 34 -1.0066729887660202 2 35 0.4181598888785345 2 36 0.3179829510321497 2 37 0.37911118185040493 2 38 1.284280238718047 2 39 1.67525076103081 2 40 3.1707788089810904 2 41 -8.248028506384468 2 42 -9.705908527772909 2 43 -14.52059313694758 2 44 -14.949316787639015 2 45 -8.599835854078307 2 46 -11.349626859199642 2 47 -17.952655108089644 2 48 -10.459142319606624 2 49 -12.600687193018093 2 50 -9.914364107221939 2 51 -5.611476009947623 2 52 -5.172793171258199 2 53 -4.7862861475963285 2 54 -5.33743005265335 2 55 -4.230515077466073 2 56 -1.6303671370929784 3 1 0.3375102329842292 3 2 0.5265326733074005 3 3 0.8605400970932493 3 4 0.8885835994828497 3 5 0.4552959823122933 3 6 0.6836471377854423 3 7 0.6984191607199577 3 8 0.5795940239733497 3 9 0.7919296337954833 3 10 0.5845366316696925 3 11 1.6426530935133457 3 12 3.4172479217296328 3 13 2.3077696255382207 3 14 2.9964831363482887 3 15 2.120506883347197 3 16 3.8909665424982123 3 17 3.5211389320483804 3 18 3.8792042124085846 3 19 3.5439296319984734 3 20 3.399397943845905 3 21 3.411134913299385 3 22 3.8481225807202932 3 23 3.2428538265074636 3 24 3.806191756281237 3 25 2.6748213903850626 3 26 2.1055814262368955 3 27 2.0088969655695976 3 28 2.5458874114866177 3 29 2.200484654605013 3 30 2.445848823888916 3 31 0.8815698265624548 3 32 1.5076306290309773 3 33 0.6021369781229069 3 34 0.5840197663899858 3 35 0.6511054203433702 3 36 2.0125889795884717 3 37 2.543574428809684 3 38 1.9498011271368725 3 39 2.7283972443729567 3 40 6.112872939070989 3 41 7.038327918787399 3 42 3.8760586853418424 3 43 -1.1155164579417622 3 44 -0.99558860149412 3 45 3.405716763584305 3 46 2.049897202143562 3 47 -2.029069703541783 3 48 2.4871823763214564 3 49 3.895346651508614 3 50 1.5911885104406736 3 51 2.4392907801105466 3 52 3.273002703940108 3 53 3.8054642490192165 3 54 4.579545092772353 3 55 3.769749332898815 3 56 3.920399652965191 4 1 0.5536056130140352 4 2 1.0590711730590172 4 3 1.1382350797062628 4 4 1.143427116621453 4 5 0.6843074080450364 4 6 1.0862800240248873 4 7 0.9256918879926843 4 8 1.0338910930245149 4 9 1.3085714619127202 4 10 1.0626787081724203 4 11 1.148614345376238 4 12 3.794793873046075 4 13 3.397188403481586 4 14 2.8519227786731776 4 15 2.896214782005913 4 16 4.2729834327118255 4 17 3.964258653856625 4 18 5.288046736005203 4 19 3.8462147785459138 4 20 4.306501768982521 4 21 3.380831882996354 4 22 3.37569337058617 4 23 2.711308868732999 4 24 3.7671952336781587 4 25 2.9505292890437786 4 26 2.357195931949761 4 27 1.751569593488128 4 28 2.1718188570901944 4 29 2.101875613373025 4 30 1.6229476813156438 4 31 1.75538999546459 4 32 1.6646102614204423 4 33 1.6634881952118263 4 34 1.127735613384516 4 35 1.2993915604327881 4 36 2.568227330308794 4 37 2.6801864506676125 4 38 1.0056878633514827 4 39 2.903012246856811 4 40 5.506067176527523 4 41 9.163017436919535 4 42 5.188030866166482 4 43 6.875790049758191 4 44 6.0702335544919706 4 45 5.106909013956884 4 46 5.521829641289116 4 47 5.799544305400097 4 48 6.224142137871379 4 49 8.30791495751954 4 50 5.398441366873854 4 51 5.912961917716114 4 52 5.93668874467533 4 53 5.465424507340135 4 54 6.772540621833457 4 55 5.313961950881925 4 56 7.075888972388938 5 1 1.1899692493776683 5 2 1.5757130011762541 5 3 1.778572883978491 5 4 1.232845894564818 5 5 1.4061156544434468 5 6 1.1426635312280666 5 7 1.5508781771133968 5 8 1.0877907452848206 5 9 1.8575034042872858 5 10 1.9943726972434632 5 11 2.9213416181035115 5 12 4.613969233205047 5 13 3.914327002587399 5 14 4.275171660938447 5 15 5.448375735806216 5 16 5.202441952334283 5 17 5.242450407458215 5 18 6.043138638638087 5 19 4.7898312713427345 5 20 4.481365156960663 5 21 3.6242496673977485 5 22 3.405499629900625 5 23 3.5215423510976294 5 24 3.8553720841500905 5 25 3.3141656526801455 5 26 2.3678765082041053 5 27 2.566274014749929 5 28 1.4475267557489104 5 29 2.9217961300148545 5 30 3.101586528806955 5 31 2.0618976954149133 5 32 2.3436962028014676 5 33 0.7004976338606141 5 34 1.8791017336031004 5 35 1.0937283711630386 5 36 2.1765234058179814 5 37 2.82226295339985 5 38 2.113735553366382 5 39 2.8759382279795176 5 40 5.981725398087384 5 41 9.103901689279201 5 42 5.7449111443582375 5 43 6.11399173877955 5 44 5.725722873915714 5 45 4.290962665223649 5 46 4.771208677553396 5 47 4.65945488662215 5 48 4.978985655009982 5 49 7.17403517609878 5 50 4.427254084211164 5 51 4.750766189946614 5 52 4.436937130169614 5 53 4.838251134265121 5 54 4.350036896051041 5 55 4.8517165460135665 5 56 5.608924243129124 6 1 0.8390920563952093 6 2 1.1069180630111717 6 3 1.5752366746025928 6 4 1.4209390783439417 6 5 0.9203520651423247 6 6 0.833488955444345 6 7 0.8563138975620603 6 8 0.8640346336943736 6 9 1.2431807123114424 6 10 1.1591699362425985 6 11 2.1669556531912306 6 12 3.979801847527739 6 13 4.29511503825033 6 14 4.190040801001722 6 15 4.321254654414211 6 16 5.087975458230169 6 17 4.973828031847056 6 18 6.005750085287499 6 19 3.256900583968566 6 20 4.55211580407024 6 21 2.5514857904923645 6 22 3.3804780595813853 6 23 2.542249857568727 6 24 3.2177534473942657 6 25 2.9106568807503237 6 26 2.5286472876116406 6 27 3.0051581102345395 6 28 2.720463195208211 6 29 3.7198979419216656 6 30 2.2186414612199528 6 31 2.5879258806320564 6 32 2.4604635309579237 6 33 2.38039409632826 6 34 3.1189636835599543 6 35 2.576903522155277 6 36 3.631225735412457 6 37 3.662642591018489 6 38 3.8103130627135258 6 39 4.906999487686157 6 40 7.439878978760376 6 41 4.196510259886047 6 42 5.337951121349899 6 43 6.418852250715123 6 44 6.978527015417015 6 45 6.1842614860462 6 46 5.489931714654347 6 47 6.252494863613812 6 48 5.4115424568506505 6 49 6.843002676817786 6 50 5.703066566235897 6 51 6.3619252351004825 6 52 6.791553178487128 6 53 6.941501062364058 6 54 7.411296602694698 6 55 7.823531328872356 6 56 7.843034107955127 7 1 0.6727278700673267 7 2 1.2560408700287127 7 3 1.4605966262057422 7 4 1.1042421636433915 7 5 0.5092812847430466 7 6 1.2294357189047176 7 7 1.4742812296854737 7 8 1.1211429216556539 7 9 1.5081534890809536 7 10 1.4304948001264464 7 11 2.90410023879317 7 12 3.8146475825549615 7 13 4.436373356459647 7 14 4.177941587450604 7 15 4.742609766331938 7 16 4.747382596766165 7 17 5.242733053020679 7 18 5.575925523883992 7 19 4.496162531941941 7 20 4.197306366683669 7 21 4.152514223644211 7 22 3.443091489708422 7 23 3.93335693560865 7 24 4.070748002748168 7 25 4.10726910095601 7 26 3.533789453370865 7 27 3.605279102370055 7 28 2.497837665867621 7 29 3.333328068963411 7 30 3.080105466059635 7 31 2.712547780208581 7 32 3.283210052434029 7 33 2.374042009213916 7 34 2.9093448087868197 7 35 3.2774479867650754 7 36 3.9987393470277013 7 37 3.3640944966446185 7 38 4.10129914861794 7 39 4.726418725435707 7 40 7.461657563152393 7 41 0.7669881788213146 7 42 4.638919058433988 7 43 5.769729443697582 7 44 5.54567765062572 7 45 5.285592399536821 7 46 5.831129536795906 7 47 6.208635214491004 7 48 5.72884433222875 7 49 7.606765532232185 7 50 5.264167594669047 7 51 4.9986463482281245 7 52 4.185382579576061 7 53 5.134181038165625 7 54 6.330534352240981 7 55 5.09818347648276 7 56 6.428031083151737 8 1 0.6808783402867604 8 2 1.0833135973014407 8 3 1.4715684130395985 8 4 0.8525180257123566 8 5 1.478246801984426 8 6 1.3802194179642768 8 7 0.49841916071995485 8 8 0.9217698809033052 8 9 1.5388744922157542 8 10 1.1505574960512135 8 11 2.303159799921694 8 12 3.891763570015776 8 13 4.124461130754312 8 14 3.7246500514004452 8 15 4.050760236551369 8 16 4.769953129681525 8 17 4.333955623553599 8 18 4.966834614793083 8 19 3.6431844755156106 8 20 4.354986617467368 8 21 3.8838621860266613 8 22 3.521147916040711 8 23 2.335551292975424 8 24 3.7278012942842196 8 25 3.1687111072255973 8 26 2.935983810737639 8 27 2.7212665631850967 8 28 1.4990915843629153 8 29 2.898845310342722 8 30 2.510826469194434 8 31 1.7796324197070135 8 32 3.2161254129355967 8 33 2.5392457709694014 8 34 1.9610689467178517 8 35 2.7539370149782414 8 36 2.2197424818239426 8 37 3.346853117334277 8 38 2.632960590624208 8 39 4.142406186250753 8 40 5.884855055315398 8 41 0.3387750126771145 8 42 5.0668187449543645 8 43 5.187911261879403 8 44 5.964172948431369 8 45 5.1159999230477915 8 46 5.75213267159215 8 47 6.9268170326728224 8 48 6.724142137871379 8 49 8.650339199943783 8 50 6.228744397176889 8 51 6.891749796503991 8 52 5.68820389619048 8 53 5.989666931582558 8 54 7.1816315309243635 8 55 5.098810435730407 8 56 5.59104048754045 9 1 0.7323421307335991 9 2 0.5187527344354947 9 3 0.7905206472460691 9 4 0.7135349748648991 9 5 0.1799417172386626 9 6 0.7306046259766035 9 7 0.8679106861436878 9 8 0.8780102507029923 9 9 1.476008544604042 9 10 0.8621137364210085 9 11 2.192528058781477 9 12 3.333057868937189 9 13 3.318605968966949 9 14 3.8442186184266447 9 15 3.573410467676176 9 16 4.418335256029749 9 17 3.790658242968078 9 18 4.703044167951791 9 19 3.6595172952382597 9 20 3.295202333954272 9 21 2.953507794655316 9 22 3.0968027696616645 9 23 2.8196807228675667 9 24 2.572793590123972 9 25 2.517555483188623 9 26 1.3523166304602867 9 27 1.5409892134162249 9 28 1.248860459555523 9 29 2.1293537849189903 9 30 1.3869435724301837 9 31 -0.7094276727429047 9 32 1.6034905901312797 9 33 0.16451541657803403 9 34 0.70683165858226 9 35 1.2254316220660684 9 36 1.7436253785881917 9 37 1.5502429478427544 9 38 1.8739467231357665 9 39 2.3485695144479806 9 40 4.889477551463322 9 41 2.368975320843525 9 42 3.6584982518881084 9 43 5.142610799629782 9 44 5.246145213454476 9 45 3.9089120956209804 9 46 5.008218958186909 9 47 4.827279282287613 9 48 4.374373262678777 9 49 6.293790663734232 9 50 4.382519435697688 9 51 4.0714108134531415 9 52 4.379421153509433 9 53 4.167787116482401 9 54 4.744035228921284 9 55 4.024542330953828 9 56 3.2982823981721943 10 1 0.6529322123406303 10 2 1.2004853144731555 10 3 1.7224101638813494 10 4 1.4784439516382832 10 5 0.7263949501325726 10 6 1.0391419768868388 10 7 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2.4168781456277415 116 29 2.711888788603588 116 30 3.509640698443448 116 31 1.6203438821576057 116 32 2.527587863528481 116 33 1.5989295654358102 116 34 1.5697645988917657 116 35 2.4910911651758667 116 36 2.642667383009716 116 37 3.4338096390734094 116 38 3.5135930016914045 116 39 4.65070658150767 116 40 7.0291238300189605 116 41 4.871186079870789 116 42 1.2723523417923097 116 43 -6.686792295432852 116 44 -3.713692664216854 116 45 2.887541425024075 116 46 -0.046681557656864214 116 47 1.9042873884040468 116 48 -0.011035727741266044 116 49 -5.597882143929734 116 50 0.399100128402182 116 51 0.8569671878083369 116 52 1.3075714851232867 116 53 0.16041791972484987 116 54 -6.1424791410123945 116 55 0.9490080641889058 116 56 2.3380760606629813 117 1 -0.32518226577384723 117 2 0.05907117305901721 117 3 0.25944720091838747 117 4 0.3252452984396328 117 5 0.01764074137836502 117 6 0.1923406300854893 117 7 0.15296461526541094 117 8 -0.011563452430031873 117 9 -0.3580952047539441 117 10 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-2.7357280818603833 120 32 -8.820238223428042 120 33 -1.6431993700964256 120 34 -2.0389310532821483 120 35 -2.0673795994418214 120 36 0.05046348495874042 120 37 -0.10142274473469115 120 38 -0.002149127244130966 120 39 1.1919359668150165 120 40 4.737519632117909 120 41 -0.09508078669592734 120 42 -2.205908527772909 120 43 -8.644063659750696 120 44 -6.333632694201864 120 45 -5.835097255635596 120 46 -13.617146325273056 120 47 -14.670675130336582 120 48 -9.01253497811608 120 49 -15.548406881560918 120 50 -9.23583240533095 120 51 -9.122043306944288 120 52 -10.573238110079114 120 53 -10.193405168730923 120 54 -11.893603578793504 120 55 -10.660437213172413 120 56 -6.709899951331021 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 112.0 5 17.0 6 35.0 7 38.0 8 87.0 9 146.0 10 73.0 11 100.0 12 81.0 13 83.0 14 85.0 15 114.0 16 162.0 17 173.0 18 231.0 19 295.0 20 414.0 21 461.0 22 604.0 23 657.0 24 843.0 25 836.0 26 968.0 27 1121.0 28 1254.0 29 1415.0 30 1655.0 31 1963.0 32 2238.0 33 2537.0 34 2870.0 35 3353.0 36 4252.0 37 5302.0 38 5365.0 39 3357.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 98.90993071593533 0.6882217090069284 0.3094688221709007 0.09237875288683603 8 98.2863741339492 1.0023094688221708 0.4457274826789839 0.26558891454965355 9 93.60508083140877 3.473441108545035 1.7043879907621247 1.2170900692840647 10 37.74364896073903 42.6905311778291 10.66974595842956 8.896073903002309 11 24.193995381062354 36.91454965357968 18.983833718244803 19.907621247113163 12 36.159353348729795 19.323325635103927 24.191685912240185 20.325635103926096 13 25.82932914433561 19.8738680465718 23.516909998151913 30.77989281094068 14 22.46472204900806 25.970576687683316 23.335411903277212 28.229289360031412 15 26.630082430304714 20.945633046216543 27.98694081689358 24.437343706585164 16 28.937881308929565 24.42919208726197 24.480033277870216 22.15289332593825 17 28.20323325635104 24.854503464203233 26.498845265588916 20.443418013856814 18 31.670477378230444 28.54338437377307 20.127485623224555 19.658652624771936 19 30.572703521305865 30.436290490393286 19.46775797091397 19.523248017386884 20 32.44711594035372 27.363310599930646 18.73771818286903 21.451855276846608 21 27.28637413394919 27.06004618937644 20.882217090069283 24.77136258660508 22 25.087759815242492 33.660508083140876 18.893764434180138 22.35796766743649 23 23.45958429561201 32.942263279445726 20.355658198614318 23.242494226327945 24 21.335365712834015 32.90145268943856 25.337767615880274 20.425413981847157 25 24.704387990762125 30.71362586605081 27.08314087759815 17.498845265588912 26 21.624743030051047 31.367657588986674 30.92185803709607 16.085741343866214 27 23.52614787071716 28.869006334674253 29.190363896980625 18.414481897627965 28 22.856680311755593 31.571035407849397 28.48817040172067 17.084113878674344 29 22.938471846768792 31.062128878722767 30.992814398927937 15.006584875580508 30 21.60957242844933 32.75045621491765 30.67843200665265 14.961539349980365 31 20.42094076333056 34.14194621569171 32.48544496811755 12.95166805286018 32 18.009522488790275 33.61762122682938 34.80793232561364 13.5649239587667 33 14.829812342365273 35.90948006571488 36.668440659925494 12.592266931994352 34 14.318728045849511 33.73081900536143 39.77167683490479 12.178776113884267 35 14.869330129167919 33.95337015042632 40.21073549460453 10.966564225801234 36 13.903842155118637 32.9552018113347 41.8016311253841 11.339324908162558 37 15.625867975187482 32.885843903342284 40.56800296268864 10.920285158781594 38 15.491554484580272 33.17268704094163 41.04590004402326 10.28985843045483 39 13.586076007961855 33.081053557376286 40.25829745868629 13.07457297597556 40 10.971043144551693 24.763344574950573 35.12966624026049 29.135946040237236 41 10.453497309761723 22.059953881629514 34.218433372929915 33.268115435678844 42 14.496877168632894 32.58848022206801 41.11265325005783 11.801989359241267 43 14.01421329197435 28.505289474293388 38.48931688233524 18.991180351397023 44 13.3645901030545 29.711725930739952 37.80289666697614 19.120787299229413 45 12.831612574656234 33.38580489837493 41.06440112968193 12.718181397286912 46 13.786560533135875 33.21917808219178 41.74842650870048 11.245834875971862 47 14.176138989002865 31.92403659550873 40.78643378615654 13.113390629331857 48 13.461805394901216 33.278582334705966 42.99264331652246 10.266968953870355 49 11.400046436034362 27.25563036916647 37.41351288599953 23.93081030879963 50 13.218324353074488 34.22981754272368 40.85054228430035 11.701315819901486 51 13.440685820203893 34.60379981464319 39.281742354031515 12.67377201112141 52 12.274819045857132 35.783826284022844 38.954281617834106 12.987073052285918 53 11.51585281184911 38.22031937051608 39.0580884054617 11.205739412173108 54 11.443983978885468 35.66781654434747 37.20047229875211 15.687727178014956 55 13.497301929178535 37.460339516894784 38.495564973714075 10.546793580212602 56 13.399365902200827 38.226377542755316 38.19860683622226 10.175649718821596 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 33.0 11 66.0 12 228.5 13 556.0 14 721.0 15 648.5 16 576.0 17 498.0 18 420.0 19 442.5 20 465.0 21 538.0 22 656.5 23 702.0 24 870.0 25 1038.0 26 1237.5 27 1437.0 28 1841.5 29 2460.0 30 2674.0 31 2763.0 32 2852.0 33 2717.5 34 2583.0 35 2400.5 36 2218.0 37 1997.5 38 1593.5 39 1410.0 40 1233.5 41 1057.0 42 962.5 43 868.0 44 912.5 45 957.0 46 902.5 47 859.0 48 870.0 49 901.5 50 933.0 51 1023.0 52 1113.0 53 1230.0 54 1525.5 55 1704.0 56 1784.0 57 1864.0 58 1645.0 59 1426.0 60 1426.0 61 1426.0 62 1478.5 63 1389.5 64 1248.0 65 1174.5 66 1101.0 67 1005.0 68 909.0 69 764.0 70 619.0 71 520.0 72 369.0 73 317.0 74 256.5 75 196.0 76 186.5 77 177.0 78 131.0 79 69.0 80 53.0 81 37.5 82 22.0 83 17.5 84 13.0 85 7.5 86 2.0 87 2.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.02771362586605081 14 0.0023094688221709007 15 0.2586605080831409 16 0.06466512702078522 17 0.0 18 0.0023094688221709007 19 0.11316397228637413 20 0.10392609699769054 21 0.0 22 0.0 23 0.0 24 0.0023094688221709007 25 0.0 26 0.016166281755196306 27 0.10623556581986143 28 0.14087759815242493 29 0.04387990762124711 30 0.020785219399538105 31 0.03695150115473441 32 0.07852193995381063 33 0.19168591224018475 34 0.06466512702078522 35 0.053117782909930716 36 0.03926096997690531 37 0.22170900692840648 38 0.325635103926097 39 0.21709006928406469 40 0.7043879907621248 41 0.8475750577367205 42 0.16166281755196305 43 0.23325635103926098 44 0.4988452655889145 45 0.23556581986143185 46 0.19399538106235567 47 0.03695150115473441 48 0.17090069284064666 49 0.5311778290993071 50 0.13163972286374134 51 0.3233256351039261 52 0.13163972286374134 53 0.20785219399538107 54 0.2471131639722864 55 0.279445727482679 56 0.20554272517321015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 43300.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.13856812933025 #Duplication Level Percentage of deduplicated Percentage of total 1 88.03749807899186 66.1501154734411 2 6.848009835561703 10.290993071593533 3 2.2929153219609653 5.168591224018475 4 1.0173659136314739 3.0577367205542725 5 0.5471031197172276 2.0554272517321017 6 0.3104349162440449 1.3995381062355658 7 0.23359459044106348 1.2286374133949192 8 0.1260181343168895 0.7575057736720554 9 0.07991393883510067 0.5404157043879907 >10 0.4702627939142462 5.8729792147806 >50 0.018441678192715538 1.0230946882217091 >100 0.018441678192715538 2.4549653579676676 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTGGGGGTGCTGCTGAGCACCTTTCTGGGATGTTGTCCTGGCTGTTGCCAAAA 260 0.6004618937644343 No Hit GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 233 0.5381062355658199 No Hit GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 215 0.4965357967667437 No Hit GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 134 0.3094688221709007 No Hit GCTGTGGGGTGCTGCTGAGCACCTTTCTGGGATGTTGTCCTGGCTGTTGCCAAAAA 118 0.27251732101616627 No Hit GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA 103 0.23787528868360278 No Hit GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 99 0.2286374133949192 No Hit GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 97 0.22401847575057734 No Hit GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 78 0.18013856812933027 No Hit GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 62 0.14318706697459582 No Hit GCTGTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56 0.12933025404157045 No Hit GCTGTGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51 0.11778290993071593 No Hit GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAACAAAAAAAAAAAAAAA 48 0.11085450346420324 No Hit GCTGTGGGGAACTATATAGTAATCATTAGTAGACTGTTGCCTTGTGGGGTTCCTGT 48 0.11085450346420324 No Hit GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 46 0.10623556581986143 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.006928406466512702 0.0 0.0 0.0 0.0 10 0.03002309468822171 0.0 0.0 0.0 0.0 11 0.03464203233256351 0.0 0.0 0.0 0.0 12 0.03695150115473441 0.0 0.0 0.0 0.0 13 0.046189376443418015 0.0 0.0 0.0 0.0 14 0.053117782909930716 0.0 0.0 0.0 0.0 15 0.057736720554272515 0.0 0.0 0.0 0.0 16 0.06466512702078522 0.0 0.0 0.0 0.0 17 0.06697459584295612 0.0 0.0 0.0 0.0 18 0.06928406466512702 0.0 0.0 0.0 0.0 19 0.07852193995381063 0.0 0.0 0.0 0.0 20 0.09237875288683603 0.0 0.0 0.0 0.0 21 0.10392609699769054 0.0 0.0 0.0 0.0 22 0.11316397228637413 0.0 0.0 0.0 0.0 23 0.12933025404157045 0.0 0.0 0.0 0.0 24 0.14318706697459585 0.0 0.0 0.0 0.0 25 0.16397228637413394 0.0 0.0 0.0 0.0 26 0.17782909930715934 0.0 0.0 0.0 0.0 27 0.19399538106235567 0.0 0.0 0.0 0.0 28 0.21478060046189376 0.0 0.0 0.0 0.0 29 0.22863741339491916 0.0 0.0 0.0 0.0 30 0.23094688221709006 0.0 0.0 0.0 0.0 31 0.24249422632794457 0.0 0.0 0.0 0.0 32 0.24480369515011546 0.0 0.0 0.0 0.0 33 0.2517321016166282 0.0 0.0 0.0 0.0 34 0.25635103926096997 0.0 0.0 0.0 0.0 35 0.26558891454965355 0.0 0.0 0.0 0.0 36 0.2678983833718245 0.0 0.0 0.0 0.0 37 0.2748267898383372 0.0 0.0 0.0 0.0 38 0.27713625866050806 0.0 0.0 0.0 0.0 39 0.2863741339491917 0.0 0.0 0.0 0.0 40 0.2956120092378753 0.0 0.0 0.0 0.0 41 0.3002309468822171 0.0 0.0 0.0 0.0 42 0.302540415704388 0.0 0.0 0.0 0.0 43 0.3163972286374134 0.0 0.0 0.0 0.0 44 0.3302540415704388 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAAAAC 65 0.0 50.46244 43 TGGCTCG 20 4.1178614E-4 50.051224 31 TTCGTGG 15 0.008419536 49.934963 15 GAAATGG 20 4.1654913E-4 49.93496 27 AAATGGC 20 4.1654913E-4 49.93496 28 CAGTTTT 15 0.008458316 49.877033 26 AGAAATG 20 4.1894696E-4 49.87703 26 TCGTGGA 15 0.00849723 49.819237 16 CGTGGAG 20 4.2135577E-4 49.819237 17 ACTGTAC 15 0.008536276 49.761578 10 GAAGCCG 30 1.0587519E-6 49.761578 9 CGCAGTT 15 0.008536276 49.761578 24 GACTGTA 15 0.008536276 49.761578 9 GACTGGT 15 0.008536276 49.761578 9 GAGAGTG 30 1.0587519E-6 49.761578 9 AAGTGTC 15 0.008536276 49.761578 10 AAGCCTG 15 0.008536276 49.761578 10 GGTGCAA 15 0.008536276 49.761578 9 GAAGTGT 25 2.1128782E-5 49.761574 9 GAACCCC 40 2.6921043E-9 49.761574 9 >>END_MODULE