FastQCFastQC Report
Fri 17 Jun 2016
SRR1526244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29725
Sequences flagged as poor quality0
Sequence length56
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.12447434819175779No Hit
TCGGGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.11774600504625736No Hit
TCGGGTGGGTGTGTCTGATTCTTTTGCCTACTCTCGAGATGTTCTTGTCTTAATTA310.10428931875525652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCAC203.8979246E-450.5565138
CCACAGT150.00804642550.47008541
TGTCTGG150.00810106950.3839642
GGTACCC204.0319108E-450.21258536
CGGGTAC204.0659725E-450.1273434
GAGTGTC150.00832241350.0423749
GAGTGTA252.0277705E-550.0423749
TGGTACA150.00832241350.04237410
GAAGCTT150.00832241350.0423749
GAAGTGT204.1002623E-450.0423749
GTTTTTC150.00832241350.04237410
ACCGTAA150.00832241350.04237410
GAGCTGA150.00832241350.0423749
GGTTCAC150.00832241350.04237412
GTACAAC150.00832241350.04237412
GACATGG150.00832241350.0423749
CCGTAAC150.00832241350.04237411
TAACTAA150.00832241350.04237414
GACCGTA150.00832241350.0423749
AACTAAG150.00832241350.04237415
Produced by FastQC (version 0.11.5)