FastQCFastQC Report
Fri 17 Jun 2016
SRR1526243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42769
Sequences flagged as poor quality0
Sequence length56
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG15923.7223222427459137No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG4871.1386752086791836No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG4270.9983866819425286No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG3390.7926301760621011No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG1070.25018120601370153No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG920.21510907432953774No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG880.20575650588042743No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC750.1753606584208188No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC710.1660080899717085No Hit
TGAAGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC680.15899366363487571No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC590.13795038462437748No Hit
TGAAGCGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.13561224251209988No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG430.10054011082793611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCT251.9511823E-550.4324639
CCGCACA203.9657383E-450.4324640
CTGTCTC251.9511823E-550.4324640
AGGACTG150.00820943250.25383835
TATGCTC850.050.25383835
TATGCTA150.00820943250.25383835
TATGCCC251.9926918E-550.25383835
ATGCCTG309.887972E-750.25383836
ATGCTCG850.050.25383836
ATGCTAG150.00820943250.25383836
GTCCACA150.00824787550.19457634
AAGGACT150.00824787550.19457634
ATATGCC650.050.19457234
TTCAAAG150.00828645250.13545644
CCACGAT150.00828645250.13545646
CGCTTCG150.00828645250.13545645
CACGATT150.00828645250.13545647
GCGCTTC150.00828645250.13545644
TCCACGA150.00828645250.13545645
GCTTCGG150.00828645250.13545646
Produced by FastQC (version 0.11.5)