##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526243_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42769 Sequences flagged as poor quality 0 Sequence length 56 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.71271247866445 39.0 38.0 39.0 34.0 40.0 2 37.20166475718394 39.0 38.0 39.0 33.0 40.0 3 35.80995580911408 39.0 36.0 39.0 28.0 40.0 4 35.613271294629286 39.0 36.0 39.0 23.0 40.0 5 36.76433865650354 38.0 36.0 39.0 32.0 40.0 6 37.027262737029154 39.0 38.0 39.0 33.0 40.0 7 37.384811428838645 39.0 38.0 39.0 34.0 40.0 8 37.38993196006453 39.0 38.0 39.0 34.0 40.0 9 37.44845565713484 39.0 38.0 39.0 34.0 40.0 10 36.53405503986532 39.0 35.0 39.0 31.0 40.0 11 35.76674694287919 38.0 35.0 39.0 29.0 40.0 12 34.460824428908786 38.0 33.0 39.0 23.0 40.0 13 34.24887184643082 38.0 33.0 39.0 23.0 40.0 14 34.20358203371601 38.0 33.0 39.0 23.0 40.0 15 34.14601697491174 38.0 33.0 39.0 23.0 40.0 16 33.667820150108724 38.0 32.0 39.0 22.0 40.0 17 33.676518038766396 37.0 32.0 39.0 22.0 40.0 18 33.217002969440486 37.0 31.0 39.0 20.0 40.0 19 33.82534078421286 37.0 31.0 39.0 24.0 40.0 20 33.89483036778975 37.0 32.0 39.0 24.0 40.0 21 34.761392597442075 38.0 33.0 39.0 26.0 40.0 22 34.54308026841871 38.0 33.0 39.0 25.0 40.0 23 35.08711917510346 38.0 34.0 39.0 27.0 40.0 24 34.539198952512336 38.0 33.0 39.0 25.0 40.0 25 34.821529612569854 38.0 33.0 39.0 27.0 40.0 26 35.38775748790012 38.0 35.0 39.0 28.0 40.0 27 35.334681661951414 38.0 35.0 39.0 28.0 40.0 28 34.99541724145993 38.0 35.0 39.0 27.0 40.0 29 34.980990904627184 38.0 35.0 39.0 27.0 40.0 30 34.55970445883701 38.0 33.0 39.0 26.0 40.0 31 35.014847202412966 38.0 35.0 39.0 27.0 40.0 32 34.662723000303956 38.0 34.0 39.0 26.0 40.0 33 34.84722579438378 38.0 35.0 39.0 27.0 40.0 34 34.77745563375342 38.0 34.0 39.0 27.0 40.0 35 34.530571208118026 38.0 34.0 39.0 27.0 40.0 36 34.12728845659239 37.0 33.0 38.0 27.0 39.0 37 33.79457083401529 36.0 33.0 38.0 26.0 39.0 38 33.536837428978934 36.0 32.0 38.0 25.0 39.0 39 32.99279852229419 36.0 32.0 38.0 22.0 39.0 40 29.93041689073862 35.0 25.0 38.0 10.0 39.0 41 26.731324089878182 32.0 16.0 38.0 6.0 39.0 42 30.823657321892025 35.0 29.0 38.0 11.0 39.0 43 29.281909794477308 33.0 26.0 38.0 6.0 39.0 44 29.395894222450842 34.0 26.0 38.0 5.0 39.0 45 30.48069863686315 35.0 29.0 38.0 4.0 39.0 46 30.081227056980524 35.0 27.0 38.0 4.0 39.0 47 29.639364960602304 35.0 27.0 38.0 4.0 39.0 48 29.696462390984124 35.0 27.0 38.0 4.0 39.0 49 27.331992798522293 33.0 17.0 38.0 4.0 39.0 50 29.252308915335874 34.0 26.0 38.0 4.0 39.0 51 29.484486427085038 35.0 27.0 38.0 4.0 40.0 52 29.304706680072016 35.0 27.0 39.0 4.0 40.0 53 28.817624915242348 35.0 25.0 38.0 4.0 40.0 54 27.921999579134418 34.0 20.0 38.0 4.0 39.0 55 28.281722743108325 34.0 23.0 38.0 4.0 39.0 56 26.81341625944025 33.0 17.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.9291081585159162 1 2 1.5585734564440799 1 3 3.298908075709406 1 4 3.626440143427942 1 5 1.5723387452409696 1 6 1.4029436759008647 1 7 1.1302884890655704 1 8 1.3825091349047511 1 9 0.9622951196991494 1 10 1.423635586969553 1 11 -3.5287052745853273 1 12 -14.854726689228983 1 13 -11.887781474808257 1 14 -5.497531017710422 1 15 -6.671905743578897 1 16 -22.898962718038696 1 17 -5.057854811857283 1 18 -11.380278364074748 1 19 -5.3528034670154625 1 20 -4.173567142152336 1 21 -4.820368945258917 1 22 -2.266063254160432 1 23 -2.577693397925966 1 24 -3.0613751046290645 1 25 -1.3119715482880423 1 26 2.343693292299754 1 27 2.298457476449869 1 28 2.678631447875219 1 29 2.5768792334900326 1 30 2.936207537498312 1 31 2.0160702587997434 1 32 1.5284737568712288 1 33 2.0309209933366787 1 34 1.129411038005685 1 35 2.085948381642986 1 36 2.5658259536590293 1 37 2.8537617697637927 1 38 2.7238772035447454 1 39 2.7890160650190765 1 40 5.181309300768756 1 41 -7.801695653794393 1 42 -6.932375458954716 1 43 -16.546457188684627 1 44 -21.499816973596463 1 45 -9.73308392143855 1 46 -16.917943816656873 1 47 -22.935068318527982 1 48 -13.225001148726616 1 49 -20.598357310745854 1 50 -12.564503703976397 1 51 -8.594721653732162 1 52 -8.841630279203404 1 53 -8.326004806382265 1 54 -7.425322678548099 1 55 -7.448894887664778 1 56 -1.8758244918059717 2 1 1.0565155659233199 2 2 1.1504253082959366 2 3 3.3566858534871855 2 4 3.6671808841686797 2 5 1.7167831896854153 2 6 1.3073881203453084 2 7 1.3095477483248317 2 8 1.2136202460158643 2 9 1.1274803048843296 2 10 1.7784504017843688 2 11 -0.5820386079186619 2 12 -5.9369489114512035 2 13 -3.1922259192526994 2 14 1.34320972303032 2 15 0.925872034198882 2 16 0.18325950418352477 2 17 1.3814044474019767 2 18 -5.8350931788895615 2 19 0.544233570021575 2 20 0.459025450440258 2 21 0.18851994362997004 2 22 0.793196005098828 2 23 0.1534177131851493 2 24 -0.8702639935179519 2 25 -0.23493451125100506 2 26 0.12961921822567746 2 27 1.1488278468202395 2 28 0.29048329972707165 2 29 1.1909533075641079 2 30 0.7510223523131287 2 31 -0.18244825971877532 2 32 1.5536589420564155 2 33 1.0968469192626031 2 34 0.719040667635312 2 35 0.7792817149763209 2 36 0.9658259536590279 2 37 1.4404284364304587 2 38 2.1698031294706723 2 39 2.511238287241298 2 40 4.890198189657642 2 41 -4.833547505646244 2 42 -6.56719027376953 2 43 -12.006457188684628 2 44 -14.978335492114983 2 45 -6.0901209584755875 2 46 -11.929795668508724 2 47 -17.18395720741687 2 48 -9.447223370948837 2 49 -14.395394347782892 2 50 -9.573392592865286 2 51 -5.5910179500284585 2 52 -4.674222871795994 2 53 -5.735634436011896 2 54 -4.371989345214768 2 55 -4.751117109886998 2 56 -1.4358244918059704 3 1 1.414733158515915 3 2 1.6735734564440818 3 3 3.297658075709407 3 4 3.8170651434279392 3 5 1.7723387452409725 3 6 1.8316936759008655 3 7 0.6596634890655722 3 8 1.52438413490475 3 9 1.4110451196991463 3 10 1.635510586969552 3 11 1.6106697254146738 3 12 3.035273310771018 3 13 1.0334685251917435 3 14 3.2274689822895795 3 15 3.0387192564211034 3 16 3.8829122819613033 3 17 3.965895188142717 3 18 3.9640966359252516 3 19 3.522821532984537 3 20 2.8039328578476628 3 21 2.6590060547410843 3 22 2.3620617458395685 3 23 2.604806602074035 3 24 2.377999895370934 3 25 2.899903451711957 3 26 1.8443182922997536 3 27 1.4659574764498657 3 28 1.891756447875217 3 29 1.7575042334900317 3 30 1.826832537498312 3 31 1.3360702587997437 3 32 0.8528487568712322 3 33 1.2021709933366793 3 34 1.5112860380056858 3 35 0.5709483816429852 3 36 2.309575953659028 3 37 2.5706367697637944 3 38 2.3376272035447485 3 39 3.6171410650190765 3 40 6.845059300768757 3 41 7.408929346205607 3 42 4.388249541045283 3 43 -2.225207188684628 3 44 1.571433026403536 3 45 3.7594160785614505 3 46 1.406431183343127 3 47 -3.5294433185279814 3 48 1.5249988512733843 3 49 4.3291426892541445 3 50 2.0186212960236034 3 51 2.314653346267839 3 52 1.6214947207965977 3 53 3.0189951936177337 3 54 3.0759273214519 3 55 1.0023551123352235 3 56 3.9391755081940296 4 1 0.7602192696270293 4 2 1.5842877421583665 4 3 3.2535112503125845 4 4 3.6486623656501607 4 5 1.8358308087330357 4 6 1.3470706600278461 4 7 1.3360027747798568 4 8 1.4675884999841173 4 9 0.9899141673181973 4 10 1.011254634588603 4 11 1.4497074238273697 4 12 4.0630510885487965 4 13 2.1569804299536486 4 14 3.7453261251467254 4 15 2.124284732611578 4 16 3.7229420438660625 4 17 4.233256299253831 4 18 3.5590867152903343 4 19 2.753228279016284 4 20 3.42198841340322 4 21 2.6091548642648945 4 22 2.2402859521887777 4 23 1.9232589830264146 4 24 2.8916407683868073 4 25 2.696282419965925 4 26 2.076709165315627 4 27 1.38956858756098 4 28 1.1211711304149006 4 29 0.5851332017440001 4 30 0.8409694422602172 4 31 0.9233718461013325 4 32 1.662124550522023 4 33 -0.694158371742688 4 34 -0.1566207080260611 4 35 -1.3556389199443188 4 36 1.3070957949288697 4 37 0.8848728808749087 4 38 1.3232422829098311 4 39 1.9794922554952663 4 40 5.342579142038598 4 41 8.27227260017386 4 42 4.517465810886556 4 43 5.993542811315372 4 44 3.4925639787844887 4 45 3.9389795706249444 4 46 3.5411038023907473 4 47 3.316042792583133 4 48 3.814681390955922 4 49 6.308309355920809 4 50 4.640893121420426 4 51 3.0386116796011713 4 52 3.6802744827013605 4 53 3.552725352347892 4 54 3.278804305578884 4 55 3.193327334557445 4 56 2.707032651051172 5 1 1.7719151760597782 5 2 2.2634857371458352 5 3 3.675399303779585 5 4 4.426440143427939 5 5 2.0705843592760615 5 6 1.976978763620167 5 7 1.9450253311708323 5 8 1.804263520869661 5 9 1.9072074004008996 5 10 2.2267934817063946 5 11 2.3828736727830915 5 12 4.092290854630669 5 13 3.8311658936127984 5 14 4.444574245447477 5 15 4.4200240809825075 5 16 4.83823026441744 5 17 5.38530308287956 5 18 4.97094970610069 5 19 4.91947723473892 5 20 4.129590752584505 5 21 3.782087195091961 5 22 3.8409542896992193 5 23 3.419499584530172 5 24 3.7028354216867214 5 25 3.2894319604838884 5 26 3.6072020642295755 5 27 2.822317125572674 5 28 3.105298114541881 5 29 3.458984496647929 5 30 3.5502426252176136 5 31 2.1957193816067644 5 32 3.0088246340642115 5 33 3.127061344213871 5 34 2.729761915198665 5 35 2.3143694342745675 5 36 4.606176830852007 5 37 4.7211301908164245 5 38 3.8228245719658034 5 39 5.332875714141885 5 40 8.62411631831262 5 41 10.614795574275785 5 42 7.12481752350142 5 43 8.800560355175023 5 44 8.013867236929851 5 45 7.060951166280752 5 46 6.83433688509751 5 47 6.9066860674369295 5 48 6.924121658290925 5 49 8.694274268201514 5 50 7.7628647170762335 5 51 5.687734486618716 5 52 5.3745100716737895 5 53 6.657153088354576 5 54 6.768361531978215 5 55 6.216719147422943 5 56 5.336105332755434 6 1 1.158199067606823 6 2 1.4622098200804459 6 3 3.369817166618496 6 4 4.0446219616097565 6 5 1.6087023816046084 6 6 0.6538527668099547 6 7 1.8139248527019376 6 8 1.3388727712683846 6 9 1.0877496651536944 6 10 1.2181810415150096 6 11 2.242203816323766 6 12 3.77618240168011 6 13 2.9431276161008313 6 14 3.838832618653214 6 15 4.4662760746029235 6 16 4.179219100143122 6 17 3.9221451881427143 6 18 4.316085272288888 6 19 3.0799238057118075 6 20 3.520978312393119 6 21 1.508721963831995 6 22 1.779391291294111 6 23 2.7786702384376696 6 24 1.982261259007295 6 25 1.5098466335301381 6 26 1.3673296559361177 6 27 1.223912021904411 6 28 1.9113587206024931 6 29 1.5986974153082159 6 30 1.8634802647710416 6 31 1.481524804254292 6 32 1.3775646659621401 6 33 0.725466447882134 6 34 1.062138310732962 6 35 0.7641301998248053 6 36 0.5203714082044826 6 37 0.7919435879456103 6 38 1.2438772035447485 6 39 1.3162887922918003 6 40 4.792218391677846 6 41 2.054667982569242 6 42 3.2294427228634675 6 43 5.011724629497188 6 44 4.52200120822172 6 45 4.083279714925084 6 46 4.231147092434039 6 47 2.8776589541992905 6 48 3.5840897603642965 6 49 5.950733598345057 6 50 3.670041750569059 6 51 2.050732891722383 6 52 2.9729151753420524 6 53 2.3212679208904596 6 54 2.367404594179174 6 55 1.9347414759715882 6 56 1.6187209627394843 7 1 1.1663495378262567 7 2 2.0399527667889075 7 3 3.5444253170887166 7 4 4.1333366951520745 7 5 2.3240628831720045 7 6 1.6388057448663815 7 7 1.8089091787207465 7 8 1.3611298245599244 7 9 1.459536499009488 7 10 1.8429459317971393 7 11 2.751294725414674 7 12 3.888721586633089 7 13 4.407390938984847 7 14 4.257641396082683 7 15 4.079128739179723 7 16 5.357589006099232 7 17 4.8807658777978915 7 18 5.496963015235593 7 19 4.438231015743156 7 20 3.2629845819855916 7 21 2.3513551926721163 7 22 2.7408332975637038 7 23 2.938858326211964 7 24 2.725521447095069 7 25 3.38320086550506 7 26 1.686451912989412 7 27 2.7267333385188337 7 28 2.438631447875217 7 29 2.5134309576279605 7 30 3.0913799512914153 7 31 2.991242672592847 7 32 3.4546806534229546 7 33 1.5329899588539178 7 34 2.6756179345574083 7 35 1.9114656230222948 7 36 1.6899638846935119 7 37 2.066865218039659 7 38 2.5714634104413037 7 39 2.47867123743287 7 40 5.553033438699789 7 41 -0.17135082620818665 7 42 4.161417644493561 7 43 5.9073359147636495 7 44 5.505700267782846 7 45 4.326226423389038 7 46 4.370332045412095 7 47 4.601483405609947 7 48 5.171550575411313 7 49 6.176125447874835 7 50 5.12584112360981 7 51 4.222519725578181 7 52 5.2114731690724625 7 53 5.652615883272908 7 54 7.070539390417416 7 55 6.005587870955914 7 56 6.202106542676788 8 1 1.7180736757572959 8 2 2.1951251805820107 8 3 3.578908075709407 8 4 4.374716005496907 8 5 2.306821503861663 8 6 2.328460917280175 8 7 1.6364953856172946 8 8 1.8438884452495756 8 9 1.787122705906043 8 10 2.41191144903852 8 11 2.6995705874836418 8 12 4.5266526211158435 8 13 4.079804732088299 8 14 4.516262085737857 8 15 4.648094256421103 8 16 4.736899350926819 8 17 4.087662429522027 8 18 4.945238877304561 8 19 4.955472395053505 8 20 3.676777685433869 8 21 3.6789413995686715 8 22 3.3270401941154333 8 23 3.542306602074035 8 24 3.777245585026108 8 25 3.8142353482636793 8 26 3.117486395748031 8 27 2.5198367867946914 8 28 2.2489762754614233 8 29 2.5306723369383093 8 30 2.281035123705209 8 31 1.267104741558363 8 32 2.5581289292850258 8 33 2.188162372647021 8 34 1.7618248311091307 8 35 2.2562932092291916 8 36 2.689963884693512 8 37 2.6530721145913816 8 38 3.226635824234407 8 39 3.6855677891570053 8 40 4.846136886975653 8 41 1.2596836565504361 8 42 2.368314196217696 8 43 4.803887638901578 8 44 4.109148543644917 8 45 3.205536768216625 8 46 4.9392975626534685 8 47 5.532517888368574 8 48 4.0508609202389 8 49 6.520953034081732 8 50 3.643082502920155 8 51 3.46389903592301 8 52 2.9183697207965977 8 53 4.531926228100495 8 54 5.32916008007259 8 55 3.9711051123352235 8 56 4.236589301297478 9 1 0.9824414918492508 9 2 1.544187491531801 9 3 3.0613642160602836 9 4 3.531703301322679 9 5 1.5793562891006232 9 6 1.3629436759008655 9 7 0.5239726995918872 9 8 1.2253161524486131 9 9 1.2580845933833587 9 10 0.8934601483730589 9 11 1.3828736727830915 9 12 3.0221154160341754 9 13 2.6557272971215653 9 14 3.742819859482559 9 15 2.805988993263206 9 16 3.347002194242002 9 17 3.473022381125176 9 18 4.163932162241039 9 19 2.5686000417564685 9 20 2.8664328578476628 9 21 2.0452450898288035 9 22 2.191831482681671 9 23 2.366868005582809 9 24 2.737923140984968 9 25 2.5350459955716076 9 26 1.344044169492733 9 27 0.8047732659235507 9 28 1.684245482962936 9 29 1.458984496647929 9 30 0.813400519954449 9 31 1.5465965745892163 9 32 0.8333860375729856 9 33 0.5481139757928162 9 34 0.7473057748477885 9 35 1.5073518904149168 9 36 2.3430189361151648 9 37 2.8790249276585342 9 38 2.103526326351769 9 39 2.0872616790541656 9 40 5.378502283224893 9 41 3.3691815391880624 9 42 4.107273663852304 9 43 5.747928776227653 9 44 5.838428640438625 9 45 4.253933622421101 9 46 3.518547411413305 9 47 3.134756242875529 9 48 3.011840956536542 9 49 4.92234444364011 9 50 4.131285769707816 9 51 3.0561555392502946 9 52 3.497317089217649 9 53 3.9027671234422954 9 54 4.084151005662427 9 55 3.3570700246159255 9 56 3.2659298941589405 10 1 0.8891081585159171 10 2 1.578573456444083 10 3 3.078908075709407 10 4 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2.132492620325692 114 37 1.5118570078590352 114 38 3.362924822592369 114 39 3.1461589221619306 114 40 7.882261681721136 114 41 6.732590060491322 114 42 2.5571483505690935 114 43 0.42211423988679897 114 44 -6.4121979259774164 114 45 2.4469160785614505 114 46 -3.839848578561636 114 47 -2.914115937575602 114 48 -4.748810672536141 114 49 -17.92978588217443 114 50 -4.8511703706430644 114 51 -6.937578796589303 114 52 -5.8911540887272125 114 53 -6.748861949239409 114 54 -9.229132202357626 114 55 -8.29079964956954 114 56 -4.8405863965678755 115 1 0.7667552173394441 115 2 0.8868087505617268 115 3 2.990672781591762 115 4 3.367616614016171 115 5 1.1252799217115594 115 6 1.1276495582538075 115 7 0.9326414302420432 115 8 0.926038546669453 115 9 0.8540598255815013 115 10 0.5518708810871971 115 11 -3.0428229216441522 115 12 -9.097079630405453 115 13 -7.2771932395141405 115 14 -9.309295723592772 115 15 -10.88131750828478 115 16 -7.480139188626932 115 17 -3.3190312824455184 115 18 -4.4320430699571 115 19 -2.6692740552507566 115 20 -2.8394494950935147 115 21 1.1525722312116713 115 22 1.5045249811336845 115 23 1.189365425603448 115 24 1.3715660718415208 115 25 1.5809696281825438 115 26 1.2036932922997536 115 27 0.8961045352733947 115 28 1.615102036110514 115 29 1.4698204099606187 115 30 -0.004968933089919858 115 31 0.9831290823291567 115 32 0.6920031686359351 115 33 1.3179798168660923 115 34 0.5011757438880409 115 35 1.6812424992900432 115 36 1.201120071306086 115 37 1.8914088285873234 115 38 2.2144654388388645 115 39 0.8184278297249605 115 40 4.501309300768757 115 41 5.930069052087958 115 42 2.159389246927635 115 43 -4.977045423978744 115 44 -3.6656993265376414 115 45 0.9763278432673346 115 46 -2.93088499312746 115 47 -3.206833024410333 115 48 -3.401471736961909 115 49 -13.387769075451738 115 50 -2.2139154686822806 115 51 -1.6182510654968674 115 52 -1.0816302792034023 115 53 -2.9001224534410888 115 54 -2.4994403256069226 115 55 -1.9994831229588925 115 56 2.4465284493705006 116 1 1.0354717948795482 116 2 1.5940280018986286 116 3 3.4198171666185004 116 4 3.7673492343370327 116 5 2.1359751088773393 116 6 1.7265800395372324 116 7 1.4593793981564787 116 8 1.5343273167229299 116 9 1.1695678469718729 116 10 1.895453768787732 116 11 -1.544159820039873 116 12 -13.737453961956255 116 13 -6.2477814748082565 116 14 -2.374803744983147 116 15 -4.9428148344879865 116 16 -4.084417263493243 116 17 -1.9233093573118296 116 18 -9.770278364074748 116 19 -2.492803467015463 116 20 -3.7472035057887005 116 21 2.1178128729229044 116 22 1.693027654930475 116 23 1.9513975111649415 116 24 1.4958976226436604 116 25 2.11439208807559 116 26 2.36278420139066 116 27 2.3920938400862326 116 28 1.6659041751479435 116 29 2.207788324399125 116 30 3.4134802647710387 116 31 1.4042520769815638 116 32 1.8457464841439588 116 33 0.8754664478821326 116 34 1.6121383107329592 116 35 1.127766563461165 116 36 1.9203714082044812 116 37 2.069216315218341 116 38 1.9711499308174751 116 39 2.1071978832008966 116 40 6.59221839167785 116 41 3.609213437114697 116 42 -2.0705572771365333 116 43 -13.233729915957355 116 44 -9.482544246323737 116 45 -1.5985384668930962 116 46 -4.032489271202326 116 47 -3.817795591255255 116 48 -3.7931829669084323 116 49 -7.221993674382219 116 50 -3.4345037039763966 116 51 -1.3401761991867076 116 52 -1.5134484610215857 116 53 -4.0605502609277195 116 54 -6.023504496729917 116 55 -1.05162216039205 116 56 0.29144823546675624 117 1 -0.14500948854291096 117 2 0.8279852211499659 117 3 2.72596689923882 117 4 3.0734989669573523 117 5 0.6841034511233275 117 6 0.7747083817832205 117 7 0.6091120184773402 117 8 0.514273840787105 117 9 0.3834715902873782 117 10 0.25775323402837813 117 11 -4.719293509879442 117 12 -10.655903159817218 117 13 -12.130134415984728 117 14 -13.191648664769243 117 15 -9.851905743578897 117 16 -13.980139188626932 117 17 -12.495501870680812 117 18 -13.167337187604161 117 19 -5.9045681728978145 117 20 -6.310037730387631 117 21 -0.40625129820008965 117 22 0.2692308634866265 117 23 -0.692987515573023 117 24 -0.3931398105114212 117 25 -0.7131480188762751 117 26 0.05663446877034062 117 27 0.8078692411557498 117 28 1.2621608596399199 117 29 0.20511452760767668 117 30 1.436207537498312 117 31 0.6007761411526857 117 32 1.3684737568712322 117 33 0.8473915815719764 117 34 1.2364698615350989 117 35 -0.40699279482760176 117 36 1.112884777188441 117 37 1.3914088285873234 117 38 1.2144654388388645 117 39 1.8184278297249605 117 40 5.530721065474641 117 41 4.253598463852665 117 42 -1.8994342824841297 117 43 -7.829986600449335 117 44 -5.695111091243522 117 45 0.358680784443802 117 46 -1.077943816656873 117 47 -1.618597730292688 117 48 -3.3720599722560287 117 49 -4.034827898981149 117 50 -4.213915468682281 117 51 -3.294721653732161 117 52 -4.434571455673989 117 53 -5.223651865205795 117 54 -11.528852090312807 117 55 -4.734777240605954 117 56 -4.259353903570677 118 1 0.5410000504078099 118 2 1.2445194023900257 118 3 3.078908075709407 118 4 3.372386089373883 118 5 1.3939603668625935 118 6 1.0656463786035673 118 7 0.9421803809574669 118 8 1.0484550808506938 118 9 0.8882410656450901 118 10 1.0717436950776573 118 11 -2.329786355666407 118 12 -7.120132094634389 118 13 -9.018051745078527 118 14 -9.748882369061771 118 15 -5.568121959795114 118 16 -12.579503258579237 118 17 -9.715692649695121 118 18 -11.628386472182857 118 19 -5.344154818366814 118 20 -5.511945520530716 118 21 0.2861175412275685 118 22 1.7096124215152457 118 23 1.3260903858578175 118 24 0.07267894942498998 118 25 1.5658662895497955 118 26 2.5280176166240764 118 27 2.3595385575309464 118 28 1.7359287451725152 118 29 2.8060684226792176 118 30 2.4767480780388524 118 31 2.3360702587997437 118 32 -3.401796513399038 118 33 1.941191263606953 118 34 2.319140767735412 118 35 2.459461895156501 118 36 3.330690818523891 118 37 3.368356364358391 118 38 3.784417744085289 118 39 3.7890160650190765 118 40 7.041849841309297 118 41 6.531817859719123 118 42 2.058435351856094 118 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0.11038139288842785 119 26 1.8801638805350507 119 27 1.3961045352733947 119 28 1.7621608596399199 119 29 1.0874674687841477 119 30 2.083266361027725 119 31 0.9537173176232727 119 32 -4.778585066658181 119 33 0.22974452274844737 119 34 0.8247051556527438 119 35 1.3283013228194562 119 36 2.377590659541383 119 37 2.5678794168226204 119 38 2.8615242623682775 119 39 3.2007807709014315 119 40 5.7071916537099305 119 41 1.871245522676194 119 42 -3.193551929542952 119 43 -10.682927776919922 119 44 -9.695111091243522 119 45 -8.43543686261502 119 46 -13.989708522539226 119 47 -13.03036243617504 119 48 -13.048530560491322 119 49 -10.299533781334091 119 50 -11.478621351035219 119 51 -8.353545183143925 119 52 -7.28751263214458 119 53 -10.047181276970502 119 54 -9.764146207959865 119 55 -6.823012534723599 119 56 -4.141706844747148 120 1 -0.13660612719836962 120 2 0.44143059930122774 120 3 1.828908075709407 120 4 2.5335830005707933 120 5 -0.15623268333045814 120 6 0.005800818758011417 120 7 0.19314563192271805 120 8 0.10250913490475 120 9 -0.12913345172942314 120 10 0.1779213012552674 120 11 -2.677276703156757 120 12 -6.857583832086124 120 13 -7.212067189093972 120 14 -13.89753101771042 120 15 -10.887620029293181 120 16 -16.217534146610124 120 17 -15.30642624042871 120 18 -14.984564078360464 120 19 -5.885660609872605 120 20 -11.062138570723764 120 21 -2.4860832309732004 120 22 -0.8946346827318621 120 23 -1.1005505407831109 120 24 -6.517089390343351 120 25 -0.9505429768594738 120 26 2.0608361494426077 120 27 1.742743190735581 120 28 0.4029171621609322 120 29 0.9740220906328858 120 30 0.6862075374983121 120 31 -0.9139297412002563 120 32 -8.595811957414483 120 33 1.1709209933366793 120 34 0.44655389514853994 120 35 -1.1254801897855842 120 36 0.822968810801882 120 37 0.3451903411923638 120 38 0.6010200606876026 120 39 1.0390160650190765 120 40 3.108452157911614 120 41 0.9825900604913222 120 42 -2.3238040303832896 120 43 -11.220742902970343 120 44 -7.209816973596464 120 45 -7.160226778581407 120 46 -12.68508667379973 120 47 -8.937925461385124 120 48 -9.546429720155189 120 49 -16.92978588217443 120 50 -8.113075132547824 120 51 -6.080435939446446 120 52 -7.510201707774829 120 53 -11.17743337781084 120 54 -8.419608392833815 120 55 -7.171752030521919 120 56 -2.1858244918059704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 247.0 5 9.0 6 9.0 7 37.0 8 63.0 9 58.0 10 32.0 11 26.0 12 55.0 13 55.0 14 72.0 15 100.0 16 129.0 17 163.0 18 200.0 19 270.0 20 348.0 21 414.0 22 521.0 23 627.0 24 746.0 25 816.0 26 880.0 27 1039.0 28 1134.0 29 1391.0 30 1706.0 31 1978.0 32 2291.0 33 2561.0 34 3127.0 35 3672.0 36 4619.0 37 5659.0 38 5172.0 39 2542.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.24244195562207 0.680399354672777 0.049100984357829267 0.02805770534733101 8 99.44819846150249 0.4512614276695737 0.0584535528069396 0.04208655802099652 9 98.15520587341298 1.108279361219575 0.3133110430451963 0.4232037223222428 10 43.93369028969581 30.351422759475323 18.218803338866937 7.496083611961934 11 31.46671654703173 40.587808927026586 14.456732680212303 13.488741845729383 12 31.438658841684397 13.965722836634011 19.57024947976338 35.025368841918215 13 23.367785988214386 14.972874380319896 22.058273314002435 39.601066317463285 14 21.441264496820054 19.194257388701832 35.154788627010845 24.209689487467266 15 34.54362195865173 17.21438282312128 23.669776381791756 24.572218836435237 16 37.58012445608946 18.144387778973474 22.065222476956908 22.210265287980164 17 25.401575907783673 19.19380859968669 33.46348991091679 21.941125581612848 18 24.703049008604562 20.302562663673775 32.5430228208006 22.45136550692106 19 37.01362550920073 22.121552652526102 19.611836868474036 21.25298496979913 20 24.196455743614955 33.23969379872182 19.364655757660884 23.19919470000234 21 24.983048469685986 19.881222380696297 32.34118169702354 22.79454745259417 22 23.83502069255769 20.108022165587226 22.4157684304052 33.641188711449885 23 24.237181135869438 21.847599897121746 21.10874698964203 32.80647197736678 24 23.559119923308938 22.277818045780823 32.61474432416002 21.54831770675022 25 35.273211905819636 20.755687530688117 21.74939792840609 22.22170263508616 26 22.054387728856362 20.085580003273552 25.267145228797904 32.59288703907218 27 22.455524344569287 17.65917602996255 25.449438202247194 34.43586142322098 28 22.13220315170815 29.999765846348375 27.363195729037393 20.50483527290608 29 33.1625970624006 19.311909439610815 26.889793245392458 20.635700252596127 30 20.391459906928887 18.30086757243411 42.35437176998808 18.953300750648925 31 21.299436349603575 28.477208410318777 32.94197441354632 17.28138082653133 32 32.90816326530612 16.691630780752668 32.12413405729264 18.276071896648567 33 21.224614087278347 17.844510552575485 33.21543182403786 27.715443536108314 34 18.57935003860462 29.75129266980183 35.338433822325165 16.330923469268384 35 18.3155924669552 18.81857527196163 47.7903848403322 15.075447420750965 36 19.66131823922908 30.319970061280817 34.40379847499649 15.614913224493614 37 19.915651358950328 18.692596063730086 45.78725398313027 15.604498594189318 38 31.232414181204277 19.3725379853686 34.86916150815982 14.525886325267304 39 20.493243084994262 17.659320326955054 34.49188467573834 27.355551912312343 40 14.940309402152158 14.88850691092326 39.198003249429 30.973180437495586 41 14.160398047492158 13.382224632725729 37.314594288678755 35.142783031103356 42 28.15390740263694 16.980866958619238 35.837568206833566 19.02765743191026 43 15.142991092358182 15.084388185654007 45.075011720581344 24.69760900140647 44 15.684200629906453 16.57030038076435 34.35105532835049 33.39444366097871 45 17.674767406435286 17.82006514963324 46.58433127885449 17.920836165076985 46 16.74163621028957 19.05866366788492 35.98303814075532 28.21666198107019 47 16.927467078333684 27.42029799078427 37.47339367997567 18.17884125090637 48 18.599367607448176 28.85349572549479 36.1658273802553 16.381309286801734 49 14.275977154676006 23.167320845182974 32.99410064164336 29.56260135849766 50 27.807699512926188 17.703259647808167 36.528194080179844 17.9608467590858 51 18.445121951219512 28.618667917448406 36.897279549718576 16.03893058161351 52 18.645099370303612 18.792574732554602 45.129801727568534 17.432524169573256 53 18.294682595455612 19.53150620754275 44.930897165612556 17.242914031389084 54 17.209291423481705 28.22117525724867 32.77547289220168 21.79406042706795 55 17.52555565975804 29.29522648410391 35.83419300384507 17.345024852292976 56 28.33848748945741 19.841158279448972 33.16230906194358 18.658045169150032 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 14.0 11 28.0 12 47.0 13 95.0 14 124.0 15 161.0 16 198.0 17 250.5 18 303.0 19 359.0 20 415.0 21 476.0 22 574.5 23 612.0 24 777.5 25 943.0 26 1061.5 27 1180.0 28 1292.5 29 1521.5 30 1638.0 31 1563.5 32 1489.0 33 1322.0 34 1155.0 35 1002.0 36 849.0 37 756.5 38 606.5 39 549.0 40 530.5 41 512.0 42 546.0 43 580.0 44 672.5 45 765.0 46 1793.5 47 2764.0 48 2706.0 49 2228.5 50 1751.0 51 1631.0 52 1511.0 53 1626.5 54 1679.5 55 1617.0 56 1644.0 57 1671.0 58 1830.0 59 1989.0 60 2178.0 61 2367.0 62 2296.0 63 2104.0 64 1983.0 65 1832.0 66 1681.0 67 1513.0 68 1345.0 69 1164.5 70 984.0 71 895.5 72 714.5 73 622.0 74 525.5 75 429.0 76 335.5 77 242.0 78 189.0 79 110.5 80 85.0 81 57.5 82 30.0 83 19.0 84 8.0 85 6.0 86 4.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.01169071056138792 14 0.0023381421122775845 15 0.25018120601370153 16 0.053777268582384435 17 0.0 18 0.0023381421122775845 19 0.12859781617526714 20 0.12158338983843439 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.004676284224555169 27 0.11456896350160162 28 0.14496481096121022 29 0.030395847459608598 30 0.014028852673665505 31 0.02805770534733101 32 0.09586382660338096 33 0.18237508475765157 34 0.06546797914377235 35 0.05611541069466202 36 0.03507213168416377 37 0.20809464799270502 38 0.2829151955855877 39 0.16834623208398608 40 0.7014426336832752 41 0.8464074446444856 42 0.15899366363487571 43 0.25485749023825666 44 0.5214056910379012 45 0.22913792700320323 46 0.19874207954359463 47 0.03741027379644135 48 0.17302251630854124 49 0.5190675489256237 50 0.15197923729804297 51 0.3016203324838084 52 0.1169071056138792 53 0.18471322686992916 54 0.24784306390142394 55 0.2735626271364774 56 0.19874207954359463 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 42769.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.03598400710796 #Duplication Level Percentage of deduplicated Percentage of total 1 86.08683871841767 59.430896209871634 2 8.141976563029194 11.241787275830625 3 2.4757840547314234 5.127545652224742 4 1.1684616947774844 3.2266361149430662 5 0.5114136693084061 1.7652972947695762 6 0.4335162229899072 1.7956931422291846 7 0.2844950213371266 1.3748275620192196 8 0.18627650206597574 1.028782529402137 9 0.11853959222380275 0.7365147653674391 >10 0.5520558152137099 6.1750333185251 >50 0.02370791844476055 1.1947906193738456 >100 0.0135473819684346 3.179873272697514 >500 0.0 0.0 >1k 0.00338684549210865 3.7223222427459137 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1592 3.7223222427459137 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 487 1.1386752086791836 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 427 0.9983866819425286 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 339 0.7926301760621011 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 107 0.25018120601370153 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 92 0.21510907432953774 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 88 0.20575650588042743 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 75 0.1753606584208188 No Hit TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 71 0.1660080899717085 No Hit TGAAGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 68 0.15899366363487571 No Hit TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 59 0.13795038462437748 No Hit TGAAGCGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58 0.13561224251209988 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG 43 0.10054011082793611 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.004676284224555168 0.0 0.0 0.0 0.0 11 0.009352568449110336 0.0 0.0 0.0 0.0 12 0.009352568449110336 0.0 0.0 0.0 0.0 13 0.014028852673665505 0.0 0.0 0.0 0.0 14 0.014028852673665505 0.0 0.0 0.0 0.0 15 0.01636699478594309 0.0 0.0 0.0 0.0 16 0.025719563235053425 0.0 0.0 0.0 0.0 17 0.037410273796441344 0.0 0.0 0.0 0.0 18 0.0444247001332741 0.0 0.0 0.0 0.0 19 0.053777268582384435 0.0 0.0 0.0 0.0 20 0.058453552806939604 0.0 0.0 0.0 0.0 21 0.06079169491921719 0.0 0.0 0.0 0.0 22 0.06780612125604994 0.0 0.0 0.0 0.0 23 0.07248240548060511 0.0 0.0 0.0 0.0 24 0.07949683181743786 0.0 0.0 0.0 0.0 25 0.08183497392971545 0.0 0.0 0.0 0.0 26 0.08183497392971545 0.0 0.0 0.0 0.0 27 0.08183497392971545 0.0 0.0 0.0 0.0 28 0.08417311604199303 0.0 0.0 0.0 0.0 29 0.08417311604199303 0.0 0.0 0.0 0.0 30 0.08651125815427062 0.0 0.0 0.0 0.0 31 0.08651125815427062 0.0 0.0 0.0 0.0 32 0.08651125815427062 0.0 0.0 0.0 0.0 33 0.0888494002665482 0.0 0.0 0.0 0.0 34 0.0888494002665482 0.0 0.0 0.0 0.0 35 0.0888494002665482 0.0 0.0 0.0 0.0 36 0.0888494002665482 0.0 0.0 0.0 0.0 37 0.09118754237882579 0.0 0.0 0.0 0.0 38 0.09352568449110336 0.0 0.0 0.0 0.0 39 0.09586382660338096 0.0 0.0 0.0 0.0 40 0.10054011082793612 0.0 0.0 0.0 0.0 41 0.1028782529402137 0.0 0.0 0.0 0.0 42 0.10989267927704646 0.0 0.0 0.0 0.0 43 0.11223082138932404 0.0 0.0 0.0 0.0 44 0.11690710561387921 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTGTCT 25 1.9511823E-5 50.43246 39 CCGCACA 20 3.9657383E-4 50.43246 40 CTGTCTC 25 1.9511823E-5 50.43246 40 AGGACTG 15 0.008209432 50.253838 35 TATGCTC 85 0.0 50.253838 35 TATGCTA 15 0.008209432 50.253838 35 TATGCCC 25 1.9926918E-5 50.253838 35 ATGCCTG 30 9.887972E-7 50.253838 36 ATGCTCG 85 0.0 50.253838 36 ATGCTAG 15 0.008209432 50.253838 36 GTCCACA 15 0.008247875 50.194576 34 AAGGACT 15 0.008247875 50.194576 34 ATATGCC 65 0.0 50.194572 34 TTCAAAG 15 0.008286452 50.135456 44 CCACGAT 15 0.008286452 50.135456 46 CGCTTCG 15 0.008286452 50.135456 45 CACGATT 15 0.008286452 50.135456 47 GCGCTTC 15 0.008286452 50.135456 44 TCCACGA 15 0.008286452 50.135456 45 GCTTCGG 15 0.008286452 50.135456 46 >>END_MODULE