FastQCFastQC Report
Fri 17 Jun 2016
SRR1526242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97234
Sequences flagged as poor quality0
Sequence length56
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5980.6150112100705515No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3310.3404159039019273No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2350.24168500730197257No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2190.2252298578686468No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1700.17483596272908655No Hit
AAAGTTGGGACTGCCCAGCTCCAAACAGACTTATTTTAGTGATTTATTCTTCTCTA1620.16660838801242364No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1470.1511816854186807No Hit
AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG1150.11827138655202912No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1130.11621449287286341No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTGT150.00822940850.27192338
CGTTGTA150.00824631350.2458539
ACGATTA252.0200501E-550.21980348
GCTCTAA150.00826324450.21980348
CCTTGGT150.00826324450.21980349
CAACGAT355.0860763E-850.1677946
ACCTCCT150.00829718450.16778645
TCGACCC150.00829718450.16778643
ACCCGTT204.1069466E-450.1418236
TTCTCTA252.0512587E-550.0899750
TGCTTCA150.00839962350.0123914
AAGTGAG150.00839962350.0123932
ATATGCC150.00841678849.98658434
CGGTAGG204.170324E-449.98658431
GTAGGAT150.00843397949.96080433
TGCGGAG150.00845119549.9350530
TATTCAC150.00845119549.9350529
TCCGTCA150.00845119549.9350528
CGCCGGT150.00845119549.9350528
ACTGGAT150.00845119549.9350511