##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526242_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 97234 Sequences flagged as poor quality 0 Sequence length 56 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.59933768023531 39.0 38.0 40.0 34.0 40.0 2 37.29431063208343 39.0 38.0 40.0 33.0 40.0 3 37.12873069091059 39.0 38.0 40.0 33.0 40.0 4 36.14012588189316 38.0 36.0 39.0 29.0 40.0 5 35.69936441985314 39.0 35.0 39.0 28.0 40.0 6 37.03668469876792 39.0 38.0 40.0 32.0 40.0 7 37.07957093197852 39.0 38.0 40.0 33.0 40.0 8 37.01573523664562 39.0 38.0 40.0 33.0 40.0 9 37.002026040273975 39.0 38.0 40.0 33.0 40.0 10 36.57911841536911 39.0 36.0 40.0 31.0 40.0 11 36.30382376534957 38.0 36.0 40.0 31.0 40.0 12 34.280046074418415 38.0 33.0 39.0 21.0 40.0 13 33.99716148672275 38.0 33.0 39.0 20.0 40.0 14 34.236676471193206 38.0 33.0 39.0 22.0 40.0 15 34.0788921570644 38.0 33.0 39.0 20.0 40.0 16 33.435742641462866 38.0 32.0 39.0 19.0 40.0 17 33.48869736923299 38.0 31.0 39.0 20.0 40.0 18 32.91338420717033 37.0 30.0 39.0 18.0 40.0 19 33.907357508690374 38.0 32.0 39.0 23.0 40.0 20 33.8212456548121 38.0 32.0 39.0 23.0 40.0 21 34.628175329617214 38.0 33.0 39.0 25.0 40.0 22 34.3508340703869 38.0 33.0 39.0 23.0 40.0 23 34.71420490774832 38.0 33.0 39.0 25.0 40.0 24 34.3885677849312 38.0 33.0 39.0 23.0 40.0 25 34.49789168397886 38.0 33.0 39.0 24.0 40.0 26 35.14068124318654 38.0 35.0 39.0 27.0 40.0 27 35.07271119155851 38.0 35.0 39.0 27.0 40.0 28 35.006170681037496 38.0 35.0 39.0 27.0 40.0 29 34.859380463623836 38.0 35.0 39.0 26.0 40.0 30 34.71270337536253 38.0 34.0 39.0 26.0 40.0 31 35.035779665549086 38.0 35.0 39.0 27.0 40.0 32 34.683402924902815 38.0 34.0 39.0 26.0 40.0 33 34.83421436945924 38.0 35.0 39.0 27.0 40.0 34 34.69398564288212 38.0 35.0 39.0 27.0 40.0 35 34.61624534627805 38.0 35.0 39.0 27.0 40.0 36 33.887076537013805 38.0 33.0 39.0 26.0 40.0 37 33.68403027747496 37.0 33.0 39.0 25.0 40.0 38 33.423565830882204 37.0 32.0 39.0 23.0 40.0 39 33.10517925828414 36.0 32.0 39.0 22.0 40.0 40 29.869438673714956 35.0 25.0 38.0 8.0 40.0 41 26.598463500421662 32.0 15.0 38.0 5.0 39.0 42 30.69262809305387 35.0 29.0 38.0 8.0 40.0 43 29.120791081309008 34.0 26.0 38.0 4.0 39.0 44 29.109519303947177 34.0 26.0 38.0 4.0 39.0 45 30.361180245593104 35.0 29.0 38.0 4.0 39.0 46 29.96531048810087 35.0 27.0 38.0 4.0 40.0 47 29.50840241067939 35.0 27.0 38.0 4.0 40.0 48 29.581401567352984 35.0 27.0 38.0 4.0 40.0 49 26.935835201678426 33.0 15.0 38.0 4.0 40.0 50 29.25236028549684 35.0 26.0 38.0 4.0 40.0 51 29.484038505049675 35.0 27.0 39.0 4.0 40.0 52 29.206841228376906 35.0 27.0 39.0 4.0 40.0 53 28.826562724972746 35.0 25.0 39.0 4.0 40.0 54 27.80256906020528 35.0 18.0 39.0 4.0 40.0 55 28.198551946849868 35.0 23.0 39.0 4.0 40.0 56 27.298187876668656 34.0 17.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.9339214122669688 1 2 1.2106017670624354 1 3 1.464672500235622 1 4 2.3732509267296606 1 5 2.826277138003718 1 6 1.9064334120445565 1 7 1.794887383817219 1 8 1.867897130299042 1 9 1.7353053405481376 1 10 1.1857140819020415 1 11 -2.137245441947911 1 12 -11.758911974952778 1 13 -7.50512119773115 1 14 -4.325254615277373 1 15 -5.5846504167354 1 16 -22.600470812491025 1 17 -3.7391550104250406 1 18 -12.494647434045604 1 19 -5.234815540637509 1 20 -5.327299966530909 1 21 -4.995959218808359 1 22 -2.106175994074693 1 23 -2.0537364921288415 1 24 -2.7878987211286343 1 25 -2.328067836180505 1 26 2.6492692881869857 1 27 2.656371812213692 1 28 2.2154635058831573 1 29 2.6233219981011615 1 30 2.916329637325454 1 31 1.8320051048737582 1 32 2.096127531971703 1 33 2.2758297731972235 1 34 2.390209270955573 1 35 2.281912387056515 1 36 2.563774878144251 1 37 2.621294072986764 1 38 2.5463356040125618 1 39 2.8307756226200738 1 40 4.712191546447343 1 41 -9.20629277103965 1 42 -9.005552329269946 1 43 -16.167422885921418 1 44 -21.101444854801542 1 45 -10.659529667516853 1 46 -16.429318202640474 1 47 -22.411382217820055 1 48 -13.787689624498155 1 49 -19.23700945549635 1 50 -12.991132017442823 1 51 -9.459037599512403 1 52 -9.484361916229915 1 53 -9.212887743540932 1 54 -8.543570287157895 1 55 -8.636556445275819 1 56 -4.922922214499636 2 1 1.551020425785424 2 2 1.9676350151442747 2 3 2.048949689372151 2 4 3.060073481838664 2 5 3.2637999732247636 2 6 2.522679910643994 2 7 2.3905517365148157 2 8 2.426172614405729 2 9 2.442527359790617 2 10 2.4013394624890623 2 11 1.9654826057929213 2 12 0.5589543019927845 2 13 -0.2402326336098497 2 14 3.4405473576857233 2 15 3.574343615660169 2 16 3.6443836510335146 2 17 4.069090026720254 2 18 -0.2606929826998474 2 19 3.2450285709201623 2 20 3.3606186791882493 2 21 2.888525009665642 2 22 2.9045413363357326 2 23 3.0351077412166703 2 24 2.120638606846036 2 25 2.8861569841824206 2 26 3.2129643314216665 2 27 3.308180361720453 2 28 2.9732274810384354 2 29 3.037948718354478 2 30 3.3860653577005593 2 31 2.472304702973865 2 32 2.9239195183314663 2 33 1.7075067918307667 2 34 2.318110866376351 2 35 2.3367169175643667 2 36 2.7804354554186403 2 37 3.2063019891967244 2 38 3.3382001759282858 2 39 3.773596229123541 2 40 5.742029568612736 2 41 -4.95894165668086 2 42 -5.146034609138415 2 43 -11.55275962931443 2 44 -12.008459834706548 2 45 -4.258238716353777 2 46 -8.684281057347572 2 47 -16.40413584100846 2 48 -6.653601206522268 2 49 -11.061513170025638 2 50 -6.0514170010014645 2 51 -0.977671139885075 2 52 -0.7477281322815514 2 53 -1.0783121741827522 2 54 0.16219027793770735 2 55 -0.3599762479795068 2 56 2.8370948357987444 3 1 1.574269218055953 3 2 2.1820069958206076 3 3 2.2014255347827785 3 4 3.356269134012578 3 5 3.6433289587320132 3 6 2.835179910643994 3 7 2.655648354872305 3 8 2.7966436288984795 3 9 2.597116731771294 3 10 2.507317723358625 3 11 2.7287676299475123 3 12 4.991321451751233 3 13 4.1571103615592335 3 14 3.9634942175891084 3 15 3.4762155963364947 3 16 5.283574472289551 3 17 5.7668557272033425 3 18 6.348606534208368 3 19 4.761332918746248 3 20 4.89503896904332 3 21 4.623730323675304 3 22 4.884311867736699 3 23 4.5933806880765715 3 24 4.383319766266325 3 25 4.540746356163098 3 26 4.047203461856455 3 27 3.833542680561031 3 28 3.4991936646133155 3 29 4.1819704574849155 3 30 3.9808721209855875 3 31 3.18033610394005 3 32 3.4429412574618965 3 33 3.4276154874829388 3 34 2.9213717359415625 3 35 3.3312821349556714 3 36 4.077838837061151 3 37 4.913427593061456 3 38 4.655229161435528 3 39 5.078849852311947 3 40 9.062681742525776 3 41 9.335140468923004 3 42 5.623590994726321 3 43 0.5985084866276011 3 44 3.1210691508006967 3 45 5.907522153211442 3 46 3.2828083146331046 3 47 -1.598882217820055 3 48 3.8294905809173443 3 49 6.196639003887405 3 50 4.39483903764588 3 51 6.028065575090768 3 52 5.566583461921347 3 53 5.633342415189226 3 54 6.610257910787951 3 55 5.585373993566389 3 56 5.691865367199711 4 1 1.623806255092994 4 2 2.223673662487272 4 3 2.278740349597591 4 4 3.057658022901464 4 5 3.1947178476209004 4 6 2.5300873180514074 4 7 2.3306483548723023 4 8 2.528588073342924 4 9 2.5378574725120373 4 10 2.5522251307660326 4 11 3.043582444762329 4 12 4.839469599899381 4 13 4.903406657855527 4 14 4.613494217589107 4 15 4.945197077817973 4 16 5.459037435252519 4 17 5.482133504981121 4 18 5.843976904578735 4 19 4.873369955783282 4 20 4.723742672747029 4 21 3.903822916267899 4 22 4.704219275144105 4 23 3.915139947335831 4 24 4.499060507007066 4 25 3.8703759857927267 4 26 2.9680367951897892 4 27 2.7613204583388082 4 28 3.707526997946651 4 29 3.5018778648923217 4 30 3.1355017506152194 4 31 2.479873140977091 4 32 3.3322931093137527 4 33 2.9753006726681264 4 34 2.7593346989045244 4 35 2.29517102384456 4 36 3.9440425407648547 4 37 4.336575741209607 4 38 4.339488420694792 4 39 4.010794296756394 4 40 7.867311372155406 4 41 10.573103431885965 4 42 6.013868772504097 4 43 8.036934412553524 4 44 6.522458039689589 4 45 6.168170301359584 4 46 6.5851231294479184 4 47 5.892321485883649 4 48 6.585046136472897 4 49 9.552657522405926 4 50 5.680950148756992 4 51 5.9155655750907705 4 52 5.677231610069498 4 53 5.597694267041078 4 54 6.86396161449165 4 55 5.480281400973791 4 56 5.433069070903414 5 1 1.5938399251266588 5 2 2.135289824103431 5 3 2.2386730095302525 5 4 2.7521024673459067 5 5 3.539162292065342 5 6 2.8029829409470253 5 7 2.45741603163998 5 8 2.3477799925348464 5 9 2.258732893387453 5 10 2.596332874873781 5 11 2.6403837915636714 5 12 5.132735593165378 5 13 4.477817432266306 5 14 4.483696237791129 5 15 4.6207863034072005 5 16 5.50011487632996 5 17 5.8609213837690035 5 18 6.044313604915434 5 19 5.257545039958366 5 20 4.8912510902554445 5 21 4.381937394382376 5 22 4.555397726322553 5 23 3.9109311931270767 5 24 4.470440978387536 5 25 4.126604942021686 5 26 2.9240974012503926 5 27 3.416876013894367 5 28 3.164597705017357 5 29 3.1687128817273376 5 30 3.820518585632051 5 31 3.3135431746471227 5 32 3.577410954431599 5 33 3.1037518511193056 5 34 2.561523251093078 5 35 3.039615468289007 5 36 4.213571160293469 5 37 4.306104360738225 5 38 3.969623100829473 5 39 4.948168034130134 5 40 7.846772651616689 5 41 10.144483903266437 5 42 6.468919277554605 5 43 8.22161454723366 5 44 7.441144908376458 5 45 6.295779728969013 5 46 6.904651748976537 5 47 6.4295268730890385 5 48 6.486056237482998 5 49 9.058381428129831 5 50 7.022364290171133 5 51 6.796373655898851 5 52 5.818477401315285 5 53 5.9862464555932675 5 54 7.27187407240411 5 55 6.601156821849212 5 56 6.594011831846178 6 1 1.4864424206703362 6 2 2.1811900023565514 6 3 2.2524876262860474 6 4 3.0633769771498294 6 5 3.6519073901045616 6 6 2.539836773389098 6 7 2.3925764594474686 6 8 2.383653432820047 6 9 2.3046330716405734 6 10 2.4472687037507868 6 11 2.9730486756991468 6 12 4.940667856979992 6 13 4.629332583781455 6 14 4.959409250268848 6 15 4.827114289146948 6 16 5.34280649843334 6 17 5.418408014785044 6 18 5.803671893685486 6 19 4.957411350118797 6 20 4.6548428906119526 6 21 4.062864310603409 6 22 4.29403408995892 6 23 4.286599642324937 6 24 3.8833197662663252 6 25 4.140419558777481 6 26 3.5116642461701773 6 27 3.585993660953193 6 28 3.4959256907571046 6 29 3.7640783006221668 6 30 3.570741402031338 6 31 2.57045048302502 6 32 3.206421649618761 6 33 3.0857037227770547 6 34 2.953234481039601 6 35 3.1180468408380264 6 36 3.7759597520938257 6 37 3.6601596192052455 6 38 4.20915073006298 6 39 4.562918479762928 6 40 7.903368017035582 6 41 4.11618622055699 6 42 6.21713674636031 6 43 7.898753584666817 6 44 7.26200052334972 6 45 5.91610058458399 6 46 6.582236419208268 6 47 7.2503824880623 6 48 7.077856593989239 6 49 8.830217435259957 6 50 6.903825965750457 6 51 6.032559039143056 6 52 6.209965814862521 6 53 5.516103853097725 6 54 6.994244838892527 6 55 5.838233470690572 6 56 5.547666020794484 7 1 1.8880612077200354 7 2 2.4691516986629836 7 3 2.623098661397762 7 4 3.5563660332373814 7 5 4.0314103540808475 7 6 2.7319822362253916 7 7 2.8071341429860013 7 8 2.9802676599062323 7 9 2.610036628412118 7 10 2.9370657853741307 7 11 2.5517650459681818 7 12 5.106308531854594 7 13 4.745611653548899 7 14 4.9835200573823855 7 15 4.971209136388175 7 16 5.907443981333479 7 17 5.6294203266865495 7 18 6.191952787438343 7 19 4.946410438126094 7 20 5.330116488423165 7 21 4.378413786207602 7 22 3.872522462051947 7 23 4.170092574381485 7 24 4.7428158902973365 7 25 4.433511214044238 7 26 4.040905012244046 7 27 3.761837254204444 7 28 3.596415886835537 7 29 3.9518347985701823 7 30 4.126543955610913 7 31 2.914670729263051 7 32 3.872689319477402 7 33 3.3622085107387534 7 34 3.5173019684997016 7 35 3.4078325225525674 7 36 3.3405972349939645 7 37 4.111425009082133 7 38 4.039919083916153 7 39 3.9136366740173756 7 40 6.724503447952138 7 41 -0.5908931228082608 7 42 5.557538023150094 7 43 7.17457437810047 7 44 6.9994606236689165 7 45 5.475836106699813 7 46 5.913460769413465 7 47 6.061485999234208 7 48 6.072384637764891 7 49 7.603131251949424 7 50 5.364154283123916 7 51 6.4778394768995575 7 52 6.493424547192667 7 53 6.485894094775794 7 54 7.016588660141956 7 55 6.091995440594811 7 56 6.103525573918059 8 1 1.2233165091487024 8 2 1.8425900995855287 8 3 1.9232460122850696 8 4 2.620559767621394 8 5 3.2485286832499156 8 6 2.302294235712864 8 7 1.8520441308135318 8 8 2.1067331605789192 8 9 2.0590083754811204 8 10 1.9806303955349378 8 11 2.484047703409402 8 12 4.833837521540033 8 13 4.488836716012862 8 14 4.335624612446772 8 15 5.0429626119471465 8 16 5.610307713795521 8 17 5.617119730876439 8 18 6.040181373510475 8 19 5.063330163925308 8 20 4.915355773451033 8 21 4.037584777302484 8 22 4.447959709793629 8 23 3.846881606350216 8 24 4.460799105109302 8 25 4.340103564611219 8 26 3.149304012820643 8 27 3.2309256006712275 8 28 2.5165260796730067 8 29 3.1280792729119113 8 30 3.493383599406158 8 31 2.3686395933799034 8 32 2.8501382271588653 8 33 2.2876361486399617 8 34 2.2454075486137413 8 35 2.6608275895011246 8 36 2.8699072484477455 8 37 3.203487280848421 8 38 3.4234798501407653 8 39 3.793898061678341 8 40 5.490023340321919 8 41 -1.3858191270365907 8 42 4.129387597113833 8 43 6.021201324093166 8 44 5.546516809202899 8 45 3.4569367537624025 8 46 3.713191693880116 8 47 3.2497748069733348 8 48 3.846937780182728 8 49 6.110723025925974 8 50 3.678011673091241 8 51 3.710974206863039 8 52 3.8315628007643205 8 53 3.8732987971910653 8 54 5.3841330255721545 8 55 4.211349659314777 8 56 4.638088966832402 9 1 1.2643586719165967 9 2 2.0645399525061023 9 3 1.9795564199051867 9 4 2.9032324108487373 9 5 3.7708007101444423 9 6 2.4746573117739388 9 7 2.024057017772492 9 8 2.3937481486724934 9 9 2.353237259078263 9 10 2.5832358024546735 9 11 2.9425152758985504 9 12 4.788873240828451 9 13 3.9683156346289152 9 14 4.818955611186091 9 15 4.767293750762107 9 16 5.812646976997648 9 17 5.366314295941578 9 18 5.887919151910811 9 19 4.243677551514608 9 20 4.158457048139368 9 21 3.5927745797958295 9 22 4.198695106908076 9 23 3.59467541500689 9 24 3.6227265459273426 9 25 3.3335900473118727 9 26 2.9098447047943026 9 27 3.442299742707924 9 28 3.387376338812942 9 29 3.2543574631346317 9 30 3.7009756991400096 9 31 3.196696744241372 9 32 3.400215268761336 9 33 3.117234131550738 9 34 3.0195355777494726 9 35 2.4689939993624535 9 36 3.8220714170988117 9 37 4.030423826583117 9 38 4.130511647311238 9 39 4.989513694119857 9 40 7.169320160604876 9 41 5.406938962331665 9 42 6.048143725423117 9 43 7.549426565723646 9 44 7.53243920729787 9 45 6.081604074115393 9 46 4.853547486007866 9 47 4.808956765230789 9 48 3.997098867169697 9 49 7.6652124502150905 9 50 4.678501937834206 9 51 3.9537953302696813 9 52 3.95111736022643 9 53 4.2725844114227485 9 54 4.929937760128816 9 55 2.9753222044891743 9 56 3.603000018800465 10 1 1.7439776661734925 10 2 2.0409372070235463 10 3 2.0472129544338316 10 4 3.55444406514205 10 5 3.3807600882085964 10 6 2.665930599349231 10 7 2.852044130813532 10 8 2.6273943176037164 10 9 2.612727383745579 10 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-1.5235481170260208 114 53 -3.277549397676271 114 54 -8.59646723541088 114 55 -2.7856055385973555 114 56 -0.4027983754903488 115 1 0.8523247736115067 115 2 1.2758958847094917 115 3 1.2683699792272236 115 4 2.2854358006792452 115 5 1.8824956253986755 115 6 1.2660132439773264 115 7 1.0728705770945268 115 8 1.027476962231816 115 9 1.3893389539935157 115 10 1.5381510566919587 115 11 -0.8490101478302634 115 12 -8.327567437137652 115 13 -6.640667416218545 115 14 -8.112061337966448 115 15 -9.274062181441284 115 16 -7.49836997215489 115 17 -1.6845331616855432 115 18 -2.417504576902747 115 19 -2.247000414587088 115 20 -2.2766276976233435 115 21 2.493452545897526 115 22 2.7690340899589216 115 23 2.61365846585435 115 24 2.3683197662663247 115 25 2.7863019117186525 115 26 2.091370128523117 115 27 1.6268760138943676 115 28 1.8597492201688723 115 29 2.1011371241515775 115 30 2.6680943432078124 115 31 1.1783916594956096 115 32 1.1237745907952288 115 33 0.9501154874829396 115 34 1.059705069274898 115 35 1.1762821349556702 115 36 1.9547832815055912 115 37 2.3039831486170144 115 38 2.4835624947688615 115 39 2.7596831856452795 115 40 5.911015075859112 115 41 5.412362691145226 115 42 0.5283132169485434 115 43 -3.985658180039067 115 44 -4.499764182532637 115 45 1.1466888198781078 115 46 -2.997469463144675 115 47 -1.9713822178200537 115 48 -2.392731641304877 115 49 -13.169194329445927 115 50 -1.0879387401318965 115 51 -1.1233233137981173 115 52 -0.9409165380786533 115 53 -2.028602029255218 115 54 -5.467519866989829 115 55 -1.608237117544725 115 56 -1.1217457439114007 116 1 1.037562868849605 116 2 1.750657789471397 116 3 1.9083699792272242 116 4 2.7973405625840044 116 5 2.867733720636771 116 6 2.2845846725487604 116 7 1.8195372437611965 116 8 2.036524581279437 116 9 1.9241008587554234 116 10 2.2305320090729097 116 11 -0.796629195449313 116 12 -12.607091246661462 116 13 -6.3611436066947356 116 14 -3.018251814156926 116 15 -5.409300276679378 116 16 -3.0912271150120354 116 17 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116 56 -1.038888601054257 117 1 0.9306968666347686 117 2 1.37612844284903 117 3 1.6269746303900092 117 4 2.707528823935057 117 5 3.058542137026585 117 6 1.7978737090936079 117 7 1.4389170887224338 117 8 1.7011978924643714 117 9 1.4007343028307275 117 10 1.324662684598934 117 11 -2.0955217757372395 117 12 -8.94407906504463 117 13 -9.800899974358082 117 14 -10.109503198431565 117 15 -8.102434274464539 117 16 -13.522788576806054 117 17 -10.998486650057636 117 18 -13.974713879228329 117 19 -4.790023670401041 117 20 -5.041976534832646 117 21 1.2311269645021738 117 22 1.7097317643775227 117 23 1.2243561402729526 117 24 1.4792499988244643 117 25 1.1893251675326084 117 26 2.1920678029417218 117 27 2.3703643859873935 117 28 2.0925399178432897 117 29 2.4983464264771627 117 30 2.9211175990217626 117 31 1.3681591013560706 117 32 1.2602862187022055 117 33 1.5482550223666607 117 34 1.304123673926064 117 35 1.3923286465835787 117 36 2.387108862900945 117 37 2.4486343114077087 117 38 2.6833299366293275 117 39 3.2741017902964487 117 40 6.503573215393992 117 41 5.118409202773133 117 42 -0.23703562026075886 117 43 -8.360309342829765 117 44 -8.841624647648914 117 45 -0.45052048244747667 117 46 -3.9935159747725812 117 47 -3.678824078285171 117 48 -4.20668512967697 117 49 -8.780589678283135 117 50 -3.7288689726900373 117 51 -2.6268116858911412 117 52 -3.615102584590282 117 53 -4.010229936231962 117 54 -10.382636146059596 117 55 -4.314981303591239 117 56 -3.9119783020509367 118 1 0.9097160779593381 118 2 1.424156754274712 118 3 1.501848240096784 118 4 2.531087974592289 118 5 2.720321712355201 118 6 1.8994915048468926 118 7 1.7637401423119172 118 8 1.860955223101378 118 9 1.8628172148630782 118 10 2.191194534952828 118 11 -1.7664014521780942 118 12 -8.441045698007219 118 13 -10.609797851001154 118 14 -11.85293090318384 118 15 -6.690149137963022 118 16 -13.620109102589673 118 17 -11.412794031250762 118 18 -12.333156750815789 118 19 -5.218739545021869 118 20 -5.0053233497972585 118 21 1.2399742850279623 118 22 2.3103384377850062 118 23 1.6075715093326082 118 24 1.693102374961974 118 25 1.7158671291099594 118 26 2.6513701285231193 118 27 2.7429629704161087 118 28 2.5710535679949587 118 29 2.5959197328472285 118 30 3.0672247779904183 118 31 1.5810003551477791 118 32 -2.22100801790042 118 33 1.8162024440046807 118 34 2.140574634492289 118 35 2.0867169175643667 118 36 3.432609368462117 118 37 2.941809235573537 118 38 3.037475538247122 118 39 3.1540310117322363 118 40 6.2601455106417205 118 41 2.628014865058269 118 42 -2.9503824352253716 118 43 -9.36435383221298 118 44 -9.171503312967417 118 45 -4.482876397513198 118 46 -10.916165115318588 118 47 -12.802686565646143 118 48 -13.240557728261399 118 49 -11.829629112054622 118 50 -9.149243087957984 118 51 -7.282018965972032 118 52 -7.283960016339524 118 53 -7.215993333603041 118 54 -12.01534595394635 118 55 -6.544758856675159 118 56 -4.3078327004331385 119 1 0.5027643340510721 119 2 0.9623794011930116 119 3 1.1479304187876593 119 4 2.121150086393527 119 5 2.3944736473766994 119 6 1.4274418154058992 119 7 1.3462771705011178 119 8 1.421872566627421 119 9 1.368789503444063 119 10 1.4979312764721797 119 11 -4.549010147830266 119 12 -12.100864140434357 119 13 -12.557480603031731 119 14 -12.100303096208208 119 15 -9.795380862759966 119 16 -14.062655686440607 119 17 -15.481236458388839 119 18 -16.12102106041923 119 19 -6.740956458543131 119 20 -7.1960782470738955 119 21 0.7092767217217002 119 22 2.2377154086402413 119 23 1.2468452790411604 119 24 1.6154626234091793 119 25 1.6072909227076693 119 26 2.989282216435207 119 27 2.8619309589493085 119 28 2.5781008685205222 119 29 2.754543717558171 119 30 3.0753470904605535 119 31 1.6115784726824174 119 32 -3.170840793820151 119 33 1.0780275753950264 119 34 1.833001772571599 119 35 1.634853563527102 119 36 3.7551129518352653 119 37 3.6626644672983346 119 38 3.8003756815820537 119 39 4.000782086744181 119 40 7.880905185749221 119 41 3.82796708674962 119 42 -1.8911373325020087 119 43 -11.107306531687417 119 44 -7.745918028686482 119 45 -4.19023425704497 119 46 -15.598678254353466 119 47 -14.14764595408379 119 48 -12.139984388557625 119 49 -12.039853670105268 119 50 -10.876070608263767 119 51 -8.140356280831085 119 52 -7.772015439177554 119 53 -8.575525106178294 119 54 -11.912025361495322 119 55 -7.948127227434835 119 56 -4.86086662303228 120 1 -0.22955022638848988 120 2 0.21152088470949337 120 3 0.5677449792272213 120 4 1.2729358006792424 120 5 1.5131206253986775 120 6 0.6997632439773298 120 7 0.31537057709452654 120 8 0.5987269622318152 120 9 0.3818389539935154 120 10 0.7000260566919607 120 11 -3.0021351478302662 120 12 -10.975692437137653 120 13 -8.964417416218545 120 14 -14.388936337966449 120 15 -12.473437181441284 120 16 -17.73899497215489 120 17 -17.700158161685543 120 18 -16.418754576902746 120 19 -7.150125414587087 120 20 -13.240377697623344 120 21 -3.904047454102475 120 22 -2.62784091004108 120 23 -2.36321653414565 120 24 -8.007305233733675 120 25 -2.3099480882813452 120 26 1.7919951285231193 120 27 1.2762510138943668 120 28 0.7734992201688726 120 29 1.1767621241515798 120 30 1.015594343207809 120 31 -0.4672333405043929 120 32 -11.879975409204768 120 33 -0.44738451251706124 120 34 -0.5577949307251018 120 35 -0.6999678650443286 120 36 0.7566582815055938 120 37 0.4377331486170135 120 38 0.37918749476886404 120 39 0.4121831856452829 120 40 3.927265075859111 120 41 0.9479876911452259 120 42 -3.1698117830514576 120 43 -9.568158180039067 120 44 -6.6497641825326355 120 45 -6.144561180121894 120 46 -15.573094463144674 120 47 -13.052007217820055 120 48 -12.502106641304877 120 49 -17.121069329445927 120 50 -11.148563740131898 120 51 -9.954573313798118 120 52 -9.699041538078653 120 53 -10.623602029255217 120 54 -11.714394866989828 120 55 -10.041362117544725 120 56 -7.3411207439114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1306.0 5 274.0 6 19.0 7 127.0 8 276.0 9 268.0 10 187.0 11 173.0 12 130.0 13 171.0 14 191.0 15 258.0 16 301.0 17 372.0 18 473.0 19 530.0 20 666.0 21 818.0 22 1017.0 23 1274.0 24 1481.0 25 1649.0 26 1921.0 27 2110.0 28 2484.0 29 2930.0 30 3550.0 31 4152.0 32 4808.0 33 5264.0 34 6317.0 35 7435.0 36 9505.0 37 12674.0 38 13360.0 39 8755.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.42098442931486 0.4278338852664706 0.13164119546660635 0.019540489952074375 8 99.35516383158155 0.5399345907810025 0.06890593825205175 0.035995639385400166 9 96.53207725692658 1.966390357282432 0.8587531110516897 0.6427792747392888 10 42.97570808564905 39.29489684678199 10.064380772157886 7.66501429541107 11 31.418022502416846 32.315856593372686 20.83838986362795 15.427731040582513 12 36.95003805253306 15.187074480120122 22.036530431741983 25.82635703560483 13 23.96671261340959 17.517024296911966 26.215359927582448 32.30090316209599 14 24.578293425491648 20.45482596889657 29.19752324528923 25.76935736032255 15 28.50411399583445 19.448167776792527 26.762625533582167 25.285092693790855 16 31.487465525048368 21.108961429218294 25.76256534804265 21.641007697690693 17 28.681325462286857 22.730732048460414 25.870580249706894 22.71736223954584 18 27.25028797103834 23.88514069442159 25.749753167681423 23.114818166858647 19 26.99308186460221 28.148163399769395 22.653804974468787 22.20494976115961 20 26.49716932578487 27.091096242923314 22.62789500772002 23.783839423571795 21 25.06839171483226 25.180492420346795 26.977189049098055 22.773926815722895 22 24.433840014809636 25.314190509492562 25.51782298372997 24.734146491967827 23 27.10471645720633 25.590842709340357 24.725918917251168 22.578521916202153 24 27.60248472756443 24.943949647242732 26.579180122179487 20.87438550301335 25 28.28772124690177 22.9726533172894 25.653841802680162 23.08578363312867 26 23.5877972517074 22.31136344935407 30.90594914835843 23.194890150580104 27 23.85919031341378 20.899056875746467 30.461060088134754 24.780692722704995 28 23.894060466265753 24.754922151742317 30.479240464618172 20.871776917373754 29 26.63655723706546 22.45347270089814 31.735270213269413 19.174699848766988 30 23.70651525437676 21.105145137731697 36.078709703964286 19.109629903927257 31 23.388606579299278 22.894850535931038 35.19863394161335 18.517908943156336 32 25.831986577043036 20.683088515342728 35.8837637806623 17.60116112695194 33 23.351362489053727 21.227012826456498 37.19672384484623 18.22490083964354 34 18.978575397723766 23.060775072547283 42.079483010557944 15.881166519171005 35 18.420538148891293 23.60137881360292 43.28239954725524 14.695683490250552 36 18.3768157688984 26.758158100489695 39.078638739146534 15.786387391465373 37 19.986603462489693 24.057089859851608 40.70795548227535 15.248351195383346 38 21.904585112307405 24.540560608873214 38.64756718849906 14.907287090320317 39 21.164844450398277 22.5147614974805 38.61381037272137 17.706583679399852 40 15.33499016257637 17.247592420006214 36.499948224086154 30.91746919333126 41 13.311486290289128 17.236694735968804 34.5781308333679 34.87368814037416 42 17.97752808988764 23.203122586226428 39.349529861275606 19.469819462610324 43 14.912524871390426 19.138341632387963 41.232383839008655 24.71674965721296 44 15.85454094395402 19.20468875979409 38.57063116330032 26.370139132951564 45 18.86240196229993 22.997248245369942 41.08874666336869 17.051603128961446 46 17.434491854630135 24.13935228595864 39.39247184411998 19.033684015291243 47 15.971807799156293 23.312069142915938 41.284082724560136 19.43204033336763 48 17.632636241887298 24.06716802307613 42.232409601318636 16.067786133717934 49 15.707548145543305 19.67089340752539 35.60287942411517 29.018679022816123 50 18.254475044801943 23.667785857004553 40.44225183842462 17.635487259768883 51 17.38291726841345 24.700845884051994 40.99442954404787 16.921807303486695 52 16.459528197946806 23.873266266462103 41.64255485651328 18.024650679077816 53 17.1841839573074 23.367605546741395 42.96458080069232 16.483629695258898 54 15.461377483170624 23.19103532880426 39.24105440037937 22.106532787645744 55 16.209779683907747 25.092528634906238 40.38578512737507 18.311906553810942 56 19.297305651434755 25.1445056926485 37.797125341301324 17.761063314615424 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 4.0 5 7.0 6 31.0 7 55.0 8 118.0 9 181.0 10 263.0 11 345.0 12 394.0 13 564.5 14 686.0 15 815.5 16 945.0 17 1062.5 18 1180.0 19 1403.0 20 1626.0 21 2004.0 22 2840.5 23 3299.0 24 3589.5 25 3880.0 26 4197.5 27 4515.0 28 4599.0 29 4160.5 30 3638.0 31 3216.0 32 2794.0 33 2533.0 34 2272.0 35 2220.5 36 2169.0 37 2233.5 38 2267.5 39 2237.0 40 2442.5 41 2648.0 42 3049.5 43 3451.0 44 3490.5 45 3530.0 46 3463.0 47 3415.5 48 3435.0 49 3654.0 50 3873.0 51 3926.5 52 3980.0 53 4137.5 54 4468.5 55 4642.0 56 4901.0 57 5160.0 58 5350.0 59 5540.0 60 4879.0 61 4218.0 62 3575.0 63 2494.5 64 2057.0 65 1764.5 66 1472.0 67 1272.0 68 1072.0 69 928.5 70 785.0 71 650.0 72 410.5 73 306.0 74 224.5 75 143.0 76 112.5 77 82.0 78 61.0 79 28.5 80 17.0 81 10.5 82 4.0 83 3.0 84 2.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 1.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.02056893679165724 14 0.01028446839582862 15 0.2550548162165498 16 0.06376370405413745 17 0.0 18 0.0020568936791657243 19 0.10078779027912048 20 0.0863895345249604 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0010284468395828621 26 0.01028446839582862 27 0.11312915235411482 28 0.12547051442910914 29 0.033938745706234445 30 0.016455149433325794 31 0.02056893679165724 32 0.08947487504370899 33 0.17380751588950366 34 0.05759302301664027 35 0.05039389513956024 36 0.030853405187485857 37 0.19951868687907523 38 0.275623753008207 39 0.19643334636032664 40 0.6828887014830204 41 0.8289281527037867 42 0.13884032334368637 43 0.24168500730197257 44 0.5059958450747681 45 0.2108316021144867 46 0.19026266532282948 47 0.04525166094164593 48 0.16866528169158934 49 0.5635888680914083 50 0.14398255754160066 51 0.3023633708373614 52 0.12032828023119484 53 0.1727790690499208 54 0.23757121994364114 55 0.24374190098113827 56 0.18409198428533227 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 97234.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.259353724006 #Duplication Level Percentage of deduplicated Percentage of total 1 86.14406244296885 63.10858341732316 2 8.049639914084764 11.794228356336262 3 2.5985147333398846 5.7109653002036325 4 1.1708056659132704 3.430898656848427 5 0.609268176266618 2.2317296418948107 6 0.3509609310291578 1.542670259374293 7 0.2372495893757107 1.2166526112265257 8 0.19092274647986185 1.118950161466154 9 0.1080959667569806 0.7127136598309234 >10 0.5124029593025704 6.268383487257544 >50 0.015442280965282943 0.714770553510089 >100 0.01123074979293305 1.53444268465763 >500 0.0014038437241166313 0.6150112100705515 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 598 0.6150112100705515 No Hit AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 331 0.3404159039019273 No Hit AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 235 0.24168500730197257 No Hit AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 219 0.2252298578686468 No Hit AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 170 0.17483596272908655 No Hit AAAGTTGGGACTGCCCAGCTCCAAACAGACTTATTTTAGTGATTTATTCTTCTCTA 162 0.16660838801242364 No Hit AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 147 0.1511816854186807 No Hit AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG 115 0.11827138655202912 No Hit AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 113 0.11621449287286341 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.01954048995207438 0.0 0.0 0.0 0.0 11 0.038052533064565894 0.0 0.0 0.0 0.0 12 0.04113787358331448 0.0 0.0 0.0 0.0 13 0.04525166094164593 0.0 0.0 0.0 0.0 14 0.05450768249789168 0.0 0.0 0.0 0.0 15 0.06170681037497172 0.0 0.0 0.0 0.0 16 0.07199127877080033 0.0 0.0 0.0 0.0 17 0.07713351296871465 0.0 0.0 0.0 0.0 18 0.08433264084579468 0.0 0.0 0.0 0.0 19 0.09564555608120616 0.0 0.0 0.0 0.0 20 0.10284468395828619 0.0 0.0 0.0 0.0 21 0.10695847131661765 0.0 0.0 0.0 0.0 22 0.11415759919369768 0.0 0.0 0.0 0.0 23 0.12547051442910917 0.0 0.0 0.0 0.0 24 0.13986877018326924 0.0 0.0 0.0 0.0 25 0.15015323857909785 0.0 0.0 0.0 0.0 26 0.16660838801242364 0.0 0.0 0.0 0.0 27 0.17483596272908652 0.0 0.0 0.0 0.0 28 0.19026266532282946 0.0 0.0 0.0 0.0 29 0.19231955900199518 0.0 0.0 0.0 0.0 30 0.19334800584157805 0.0 0.0 0.0 0.0 31 0.2026040273978238 0.0 0.0 0.0 0.0 32 0.20671781475615525 0.0 0.0 0.0 0.0 33 0.20980315527490384 0.0 0.0 0.0 0.0 34 0.21288849579365243 0.0 0.0 0.0 0.0 35 0.21803072999156672 0.0 0.0 0.0 0.0 36 0.22214451734989818 0.0 0.0 0.0 0.0 37 0.22831519838739536 0.0 0.0 0.0 0.0 38 0.22831519838739536 0.0 0.0 0.0 0.0 39 0.23345743258530965 0.0 0.0 0.0 0.0 40 0.23345743258530965 0.0 0.0 0.0 0.0 41 0.23551432626447538 0.0 0.0 0.0 0.0 42 0.24271345414155543 0.0 0.0 0.0 0.0 43 0.24682724149988688 0.0 0.0 0.0 0.0 44 0.24888413517905258 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTTGT 15 0.008229408 50.271923 38 CGTTGTA 15 0.008246313 50.24585 39 ACGATTA 25 2.0200501E-5 50.219803 48 GCTCTAA 15 0.008263244 50.219803 48 CCTTGGT 15 0.008263244 50.219803 49 CAACGAT 35 5.0860763E-8 50.16779 46 ACCTCCT 15 0.008297184 50.167786 45 TCGACCC 15 0.008297184 50.167786 43 ACCCGTT 20 4.1069466E-4 50.14182 36 TTCTCTA 25 2.0512587E-5 50.08997 50 TGCTTCA 15 0.008399623 50.01239 14 AAGTGAG 15 0.008399623 50.01239 32 ATATGCC 15 0.008416788 49.986584 34 CGGTAGG 20 4.170324E-4 49.986584 31 GTAGGAT 15 0.008433979 49.960804 33 TGCGGAG 15 0.008451195 49.93505 30 TATTCAC 15 0.008451195 49.93505 29 TCCGTCA 15 0.008451195 49.93505 28 CGCCGGT 15 0.008451195 49.93505 28 ACTGGAT 15 0.008451195 49.93505 11 >>END_MODULE