FastQCFastQC Report
Fri 17 Jun 2016
SRR1526241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143707
Sequences flagged as poor quality0
Sequence length56
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA3260.22685046657434918No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAACAAAAAAAAAAAAAAA2900.20179949480540268No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2760.1920574502285901No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2580.17953196434411683No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAACAAAAAAAAAAAAAAA2450.1704857800942195No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAACCAAAAAAAAAAAAAAA2260.15726443388283104No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA2100.14613066865218813No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAACAAAAAAAAAAAAAAA2090.14543480832527295No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAA2070.14404308767144258No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAACAAAAAAAAAAAAAAA2040.14195550669069704No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.1398679257099515No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAACAAAAAAAAAAAAAAA1980.13778034472920594No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.13360518276771485No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA1910.1329093224407997No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1760.12247141753707197No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAACAAAAAAAAAAAAAAA1730.12038383655632642No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAACAAAAAAAAAAAAAAA1710.11899211590249606No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.11829625557558086No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAACCAAAAAAAAAAAAAAA1680.1169045349217505No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA1640.11412109361408979No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAA1580.1099459316525987No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAACAAAAAAAAAAAAAAA1570.1092500713256835No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAACCAAAAAAAAAAAAAAA1530.10646663001802278No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAA1470.10229146805653169No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAAACAAAAAAAAAAAAAAA1460.10159560772961651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA150.00813014250.4388939
AACTACA150.00813014250.4388938
AAACTAC204.0442465E-450.31482737
ATGCTCG150.0082439850.2618536
AAAACCT150.0082439850.2618536
GCAAACC150.008266889550.2265935
CTCGGAC150.00828984650.1913843
CCAACAC850.050.19137643
CAACACG150.00831284950.1562244
CCGTCGC150.00835950.08604449
TCATTAC150.00840534150.01606850
GCGGATT150.00840534150.01606850
ACGGGTA204.179311E-449.98114833
TGACGGG204.2009607E-449.9288731
TTGACGG204.2009607E-449.9288730
AACGCAC150.008463534549.9288730
GACGGGT204.2009607E-449.9288732
CGCACTG150.008463534549.9288732
GAAGCTA150.0084752149.911479
GAAGCAC150.0084752149.911479
Produced by FastQC (version 0.11.5)