##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526241_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 143707 Sequences flagged as poor quality 0 Sequence length 56 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.50283563083218 39.0 38.0 40.0 33.0 40.0 2 36.46803565588315 39.0 38.0 40.0 30.0 40.0 3 35.98286096014808 39.0 35.0 40.0 28.0 40.0 4 36.06204986535103 39.0 35.0 40.0 28.0 40.0 5 36.343080016978995 39.0 37.0 40.0 30.0 40.0 6 36.80756678519487 39.0 38.0 40.0 32.0 40.0 7 36.77597472635293 39.0 38.0 40.0 31.0 40.0 8 36.6817343622788 39.0 37.0 40.0 31.0 40.0 9 36.671108575086805 39.0 37.0 40.0 31.0 40.0 10 36.027563027549114 39.0 35.0 40.0 29.0 40.0 11 35.4650017048578 38.0 35.0 40.0 28.0 40.0 12 33.1411204742984 38.0 31.0 39.0 17.0 40.0 13 32.82157445357568 37.0 31.0 39.0 16.0 40.0 14 33.52307124913887 38.0 32.0 39.0 19.0 40.0 15 33.494680147800736 38.0 32.0 39.0 19.0 40.0 16 32.96111532493198 38.0 30.0 39.0 18.0 40.0 17 32.789265658597 37.0 30.0 39.0 18.0 40.0 18 32.18445865545868 36.0 29.0 39.0 16.0 40.0 19 33.229933127822584 37.0 30.0 39.0 21.0 40.0 20 33.17905182071855 37.0 30.0 39.0 21.0 40.0 21 34.39781639029414 38.0 33.0 39.0 23.0 40.0 22 34.17492536897994 38.0 33.0 39.0 23.0 40.0 23 34.3734404030423 38.0 33.0 39.0 23.0 40.0 24 34.10000208758098 38.0 33.0 39.0 23.0 40.0 25 34.2021613421754 38.0 33.0 39.0 23.0 40.0 26 35.089118832068024 38.0 35.0 40.0 27.0 40.0 27 34.97680001670065 38.0 35.0 40.0 26.0 40.0 28 34.91747792383113 38.0 35.0 40.0 26.0 40.0 29 34.767784450305136 38.0 34.0 40.0 26.0 40.0 30 34.69430159978289 38.0 34.0 39.0 25.0 40.0 31 34.83706430445281 38.0 35.0 39.0 27.0 40.0 32 34.37673878099188 38.0 34.0 39.0 25.0 40.0 33 34.509989074992866 38.0 34.0 39.0 26.0 40.0 34 34.381380169372406 38.0 34.0 39.0 25.0 40.0 35 34.341479538227084 38.0 34.0 39.0 25.0 40.0 36 33.11117064582797 37.0 32.0 39.0 21.0 40.0 37 32.92642668763526 36.0 32.0 39.0 19.0 40.0 38 32.633406862574546 36.0 32.0 39.0 17.0 40.0 39 32.273097343901135 35.0 32.0 39.0 11.0 40.0 40 29.404607987084834 35.0 25.0 38.0 4.0 40.0 41 26.367678679535445 32.0 14.0 38.0 4.0 39.0 42 29.60670670183081 35.0 27.0 38.0 4.0 40.0 43 28.297292407467975 33.0 24.0 38.0 4.0 39.0 44 28.30832179364958 34.0 23.0 38.0 4.0 40.0 45 29.19779829792564 34.0 27.0 38.0 4.0 40.0 46 29.016638020416543 35.0 26.0 38.0 4.0 40.0 47 28.568824065633546 35.0 25.0 38.0 4.0 40.0 48 28.461752037131106 34.0 24.0 38.0 4.0 40.0 49 26.36268936099146 33.0 11.0 38.0 4.0 40.0 50 28.032997696702317 34.0 23.0 38.0 4.0 40.0 51 27.773699263083913 35.0 19.0 39.0 4.0 40.0 52 27.41299310402416 34.0 15.0 39.0 4.0 40.0 53 27.0154968094804 34.0 4.0 39.0 4.0 40.0 54 26.029316595572936 33.0 4.0 38.0 4.0 40.0 55 26.10624395471341 33.0 4.0 38.0 4.0 40.0 56 25.829173248345594 34.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.6748032571770892 1 2 1.9220642099063667 1 3 2.342432897926237 1 4 1.7311292025208331 1 5 1.854988205616543 1 6 1.7218266450021957 1 7 1.5581896041922079 1 8 1.5788414512077225 1 9 1.608918723948051 1 10 1.4590495908456305 1 11 -3.0041628358255146 1 12 -12.309878780976884 1 13 -9.419491706878805 1 14 -5.659114451286907 1 15 -6.70789817703054 1 16 -21.026913871895047 1 17 -4.113551387681838 1 18 -11.65901966311359 1 19 -5.124560601721704 1 20 -4.641127979799677 1 21 -6.239060190615142 1 22 -2.841550268819976 1 23 -2.434621009346909 1 24 -3.2148032588103845 1 25 -2.8319368818230473 1 26 2.584479450142993 1 27 2.5023674539815346 1 28 2.233012876555023 1 29 2.694137772730052 1 30 2.6159396948362783 1 31 2.04174458063585 1 32 2.192313818838329 1 33 0.91651763479463 1 34 0.9712126040759372 1 35 0.7030341324625695 1 36 0.5926430569519283 1 37 0.2670387647816952 1 38 -0.02774791760026929 1 39 -0.6358357655374007 1 40 1.4692720734473994 1 41 -14.90968910484584 1 42 -14.827420484928753 1 43 -19.15215387750225 1 44 -22.345517100439757 1 45 -15.89556243197415 1 46 -19.74007922084088 1 47 -22.874979186384124 1 48 -17.700122669775396 1 49 -19.975636481907433 1 50 -16.906839465749364 1 51 -13.883486056746461 1 52 -13.732129210046123 1 53 -13.76745073058133 1 54 -12.951197285930705 1 55 -13.255724960111063 1 56 -10.65523311311096 2 1 1.712290506412046 2 2 2.8760764506408094 2 3 3.0118480628361297 2 4 3.1471441634184814 2 5 2.732269175808092 2 6 2.5995893107621413 2 7 2.3649163411297636 2 8 2.366187025275501 2 9 2.382068446264718 2 10 2.332930357024935 2 11 1.184321806586361 2 12 -3.090367276953309 2 13 -1.0523263436236796 2 14 3.3065151531560275 2 15 2.176523221586706 2 16 2.7373669849563598 2 17 3.826066656067457 2 18 -1.268931257809271 2 19 3.055140180325221 2 20 3.243180078683835 2 21 3.3190266279273004 2 22 3.563329023937591 2 23 3.220059938176611 2 24 2.2592418505628444 2 25 2.9721830320384512 2 26 2.645881467597377 2 27 3.2273759544915706 2 28 2.878144917810829 2 29 2.653930360285308 2 30 3.017503788681907 2 31 2.224930571795319 2 32 2.588720936598726 2 33 2.5426652036487596 2 34 2.231469886179532 2 35 2.368425722624643 2 36 2.7833094969383296 2 37 2.50522305583916 2 38 2.349051357022873 2 39 2.716312029996999 2 40 3.9779992637454846 2 41 -13.7335018669449 2 42 -12.507546292477205 2 43 -17.58392311698995 2 44 -17.445114742964975 2 45 -10.677439344588908 2 46 -12.011273825850513 2 47 -17.33760527728291 2 48 -9.841344476417127 2 49 -14.898933546397968 2 50 -9.667408433220746 2 51 -3.417006401300469 2 52 -3.2646861634633275 2 53 -3.1005290486137476 2 54 -1.9042461355283464 2 55 -2.3904297090626656 2 56 0.06759358982455055 3 1 1.5674340130900788 3 2 2.714027573118784 3 3 2.875090461244767 3 4 2.7511509835378334 3 5 2.743849170124655 3 6 2.6679325901028648 3 7 2.382321854059569 3 8 2.5205632685264234 3 9 2.405796778177198 3 10 2.1782699421343423 3 11 2.6435431762936688 3 12 4.954034541745187 3 13 3.102973457456173 3 14 4.382744195497601 3 15 3.554116287798699 3 16 4.946446268843829 3 17 5.402295698409031 3 18 6.238386161912242 3 19 4.7745206861507405 3 20 4.197357401786988 3 21 4.0442326523660626 3 22 3.9713284555943105 3 23 3.6865987276054284 3 24 4.381577741440935 3 25 4.241364617716201 3 26 3.4407805866652907 3 27 3.0200017004421227 3 28 2.7721488962137855 3 29 3.028113366821252 3 30 3.1698197875452223 3 31 2.591156772420497 3 32 2.7678911554108865 3 33 2.8261264142199494 3 34 2.0373096133747595 3 35 2.0820517527468354 3 36 3.35101339008979 3 37 3.8182381169360653 3 38 3.378747293368427 3 39 3.525064516498844 3 40 6.5641032705655995 3 41 7.038734563859304 3 42 4.512558851029471 3 43 -2.45334624856142 3 44 -2.8393318500976825 3 45 3.8407476403499956 3 46 2.4223010533765397 3 47 -2.188486209365891 3 48 2.640610624463804 3 49 4.390424225696378 3 50 2.4626000919284436 3 51 4.443536366531301 3 52 4.40986626620419 3 53 4.365441397535729 3 54 4.994517717839084 3 55 4.532133519127161 3 56 5.225166764021765 4 1 1.8173651787680356 4 2 3.205930153780095 4 3 3.5254871972463704 4 4 3.217397135073469 4 5 3.2152266074854268 4 6 2.935310127586284 4 7 2.6298000148064844 4 8 2.7124483277239406 4 9 2.760255990712551 4 10 2.739081936827837 4 11 3.473359701537092 4 12 6.071803809097688 4 13 6.089957512924123 4 14 5.229531509957816 4 15 4.936835118366147 4 16 6.127568143139847 4 17 6.135093375563546 4 18 6.6185268342358405 4 19 5.642308726500165 4 20 5.754990502335161 4 21 4.10090207240561 4 22 4.473493607905894 4 23 4.366331400129269 4 24 5.111471861738295 4 25 4.723605362628717 4 26 3.718445726461546 4 27 3.7786560520228107 4 28 3.1464574241605234 4 29 3.641815151507494 4 30 3.416772304378341 4 31 2.879685219768497 4 32 2.854747554499774 4 33 2.0755819973092358 4 34 2.3231848104912274 4 35 2.2908325091734554 4 36 3.8339800242165936 4 37 3.6663019951869202 4 38 4.15282053308708 4 39 4.3337051012151235 4 40 6.996032383729226 4 41 9.808152100341498 4 42 6.394389093075976 4 43 7.467331581780748 4 44 6.830588802702 4 45 6.291624965072785 4 46 6.235271942715983 4 47 4.62518553852528 4 48 5.904058347925044 4 49 7.985578289424442 4 50 5.595062358204633 4 51 5.229874630905162 4 52 5.533317493958187 4 53 6.085974050035038 4 54 6.7512196777399645 4 55 5.387331136208083 4 56 5.1888472726448285 5 1 2.116852331141935 5 2 3.055248119308274 5 3 3.0925803557532987 5 4 3.236880057776226 5 5 2.959860212222658 5 6 2.6452075580610597 5 7 2.480862719681184 5 8 2.6405967891810604 5 9 2.646874368633668 5 10 2.3093209132474257 5 11 3.3620929150297414 5 12 5.9070316826305245 5 13 5.409958570341907 5 14 5.107135401255263 5 15 5.5280638378332085 5 16 6.194449818089616 5 17 6.2389318021258795 5 18 6.028487709777767 5 19 5.393075354394849 5 20 5.867129658515765 5 21 4.548718504678 5 22 4.354745590564242 5 23 4.698651374751243 5 24 4.635792470810941 5 25 4.108637613871181 5 26 3.3527642206986172 5 27 3.4848789556920465 5 28 3.3314262303358433 5 29 3.8841224371160408 5 30 3.58683151977435 5 31 2.404402360510801 5 32 3.1674524291957624 5 33 2.1116928146931784 5 34 2.1371321510854955 5 35 2.596717039151251 5 36 4.121544791055975 5 37 3.9229901626818986 5 38 4.161676407042883 5 39 4.152886659848939 5 40 7.252509067667052 5 41 9.482784647660939 5 42 6.264062350980179 5 43 7.46159509029296 5 44 6.986557926102652 5 45 5.880183704630781 5 46 5.653692160544047 5 47 5.563855306950785 5 48 5.2281833347073245 5 49 7.83886157164925 5 50 4.835492727243437 5 51 4.297179272969242 5 52 4.345758014207739 5 53 4.340547381958483 5 54 5.103657025736158 5 55 4.099766369368705 5 56 3.8942242420854534 6 1 1.6651091338630266 6 2 2.6269955682878674 6 3 3.1641152196988784 6 4 2.8652814183204427 6 5 3.0312887836512274 6 6 2.5600672519386123 6 7 2.175271733286621 6 8 2.221272809589223 6 9 2.321406681558841 6 10 2.0936533962406187 6 11 3.0424713163902837 6 12 5.5671082132427685 6 13 5.2571099308861235 6 14 5.128819074724653 6 15 5.068986456880829 6 16 5.721129239858321 6 17 6.106703910969419 6 18 6.621387369641713 6 19 5.452811748952673 6 20 5.305373706134816 6 21 4.1655339808684815 6 22 4.022581474917985 6 23 4.093528075431514 6 24 4.440001654484412 6 25 4.431129110469826 6 26 3.5928790166169833 6 27 3.189385758413138 6 28 3.3040027609480873 6 29 3.191307811265702 6 30 3.4231410435838683 6 31 2.223705081599242 6 32 2.3785493679712744 6 33 2.2889887330605276 6 34 2.2510777293167905 6 35 2.3690384677226817 6 36 1.9548781243893103 6 37 2.5928456048587663 6 38 2.1116126315326795 6 39 2.0410669319577863 6 40 4.611884067667052 6 41 0.7174785252119626 6 42 2.6245002761502434 6 43 3.905047471245343 6 44 2.9893215315448316 6 45 3.0566292828620725 6 46 3.561642840816152 6 47 4.456818742324934 6 48 3.887515817700521 6 49 5.811544394778501 6 50 4.370888135406705 6 51 4.637527912425021 6 52 4.196098150262163 6 53 4.468098402366646 6 54 5.04275141349126 6 55 3.4643497027020373 6 56 3.80993427609906 7 1 1.6390674671963552 7 2 2.7467872349545317 7 3 3.278698553032207 7 4 3.0996564183204427 7 5 2.9792054503178917 7 6 2.6850672519386123 7 7 2.3731883999532926 7 8 2.5285644762558874 7 9 2.446406681558841 7 10 2.6769867295739544 7 11 2.8080963163902837 7 12 5.718149879909433 7 13 5.3039849308861235 7 14 5.1704857413913174 7 15 5.397111456880829 7 16 5.705504239858321 7 17 5.830662244302747 7 18 6.3505540363083774 7 19 5.239270082286001 7 20 5.373082039468144 7 21 3.81136731420181 7 22 3.7882064749179847 7 23 4.025819742098179 7 24 4.210834987817748 7 25 3.8998791104698256 7 26 3.1189206832836476 7 27 3.1685524250798025 7 28 2.652961094281423 7 29 3.420474477932366 7 30 3.2200160435838683 7 31 2.1039134149325704 7 32 2.19625770130461 7 33 2.5077387330605276 7 34 2.058369395983455 7 35 2.316955134389346 7 36 2.9965447910559746 7 37 2.379303938192102 7 38 2.7574459648660152 7 39 3.1504419319577863 7 40 4.768134067667052 7 41 1.8997701918786305 7 42 3.4786669428169112 7 43 5.300880804578675 7 44 4.770571531544828 7 45 3.202462616195408 7 46 3.3637261741494875 7 47 3.9411937423249306 7 48 4.048974151033853 7 49 5.936544394778501 7 50 3.0323464687400374 7 51 2.361486245758357 7 52 2.810681483595495 7 53 2.921223402366646 7 54 2.74587641349126 7 55 1.7768497027020373 7 56 1.5130592760990602 8 1 1.6078174671963552 8 2 2.741578901621203 8 3 2.919323553032207 8 4 2.813198084987114 8 5 2.9531637836512274 8 6 2.4663172519386123 8 7 1.9617300666199569 8 8 2.117106142922559 8 9 2.2016150148921696 8 10 2.21344506290729 8 11 2.7143463163902837 8 12 5.587941546576104 8 13 5.5383599308861235 8 14 4.930902408057989 8 15 4.813778123547493 8 16 5.377379239858321 8 17 5.575453910969419 8 18 6.548470702975042 8 19 4.952811748952673 8 20 5.248082039468144 8 21 4.45199231420181 8 22 4.366331474917985 8 23 4.057069742098179 8 24 4.736876654484412 8 25 4.264462443803154 8 26 2.9678790166169833 8 27 3.3404274250798025 8 28 2.840461094281423 8 29 3.0558911445990304 8 30 2.6054327102505397 8 31 2.442455081599242 8 32 2.82125770130461 8 33 2.0285720663938633 8 34 2.2667027293167905 8 35 1.7492468010560174 8 36 1.9652947910559746 8 37 2.113678938192102 8 38 2.293904298199344 8 39 2.7754419319577863 8 40 4.387925734333717 8 41 1.2591451918786305 8 42 3.468250276150247 8 43 4.795672471245339 8 44 4.7341131982114995 8 45 3.900379282862076 8 46 4.264767840816155 8 47 4.300568742324934 8 48 4.590640817700518 8 49 6.150086061445169 8 50 3.6104714687400374 8 51 4.460444579091689 8 52 4.076306483595495 8 53 4.749348402366646 8 54 4.782334746824592 8 55 4.631016369368705 8 56 3.6953509427657245 9 1 1.5239300558488367 9 2 2.6432854619048882 9 3 3.0259281629612858 9 4 2.8927636878239937 9 5 2.8823526134384636 9 6 2.4181124647045706 9 7 1.8407194283220818 9 8 1.816020150014758 9 9 2.008685050353165 9 10 2.150723431701614 9 11 3.108184968872557 9 12 5.622294383455532 9 13 5.603076242942862 9 14 5.2524892874906115 9 15 4.510586634185792 9 16 6.004263105106553 9 17 5.686934407423319 9 18 6.537389142691357 9 19 5.525728415619337 9 20 5.08141537280148 9 21 3.8458309666840833 9 22 3.9050061203080517 9 23 4.228612295289665 9 24 4.544389952356752 9 25 4.315215989902448 9 26 3.375126357042518 9 27 3.104722637845761 9 28 3.0892421226502123 9 29 3.022203201336623 9 30 3.7312084194703985 9 31 2.700212173797823 9 32 3.098629155205316 9 33 2.69723341391159 9 34 2.7581699278983507 9 35 2.7752884677226817 9 36 3.265161812332572 9 37 3.2912055339367825 9 38 3.5913333762135338 9 39 3.9298080667095547 9 40 5.209955876177691 9 41 5.206840227339622 9 42 5.202403644944571 9 43 6.526944634365911 9 44 6.502841035090928 9 45 5.134200204847886 9 46 5.135889294716861 9 47 5.733525302608619 9 48 4.771713512735978 9 49 6.947958401870704 9 50 5.359917390725851 9 51 4.98172117483637 9 52 5.157091058063582 9 53 4.728736700238986 9 54 6.069679604980621 9 55 4.163596156602747 9 56 3.924185162623882 10 1 1.8328787417061605 10 2 2.855488215346689 10 3 2.8971421804831863 10 4 2.8824382810655464 10 5 2.607759371886523 10 6 2.5172363695856745 10 7 2.0874653607376032 10 8 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-3.2881182601328263 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2742.0 5 20.0 6 43.0 7 201.0 8 386.0 9 375.0 10 274.0 11 274.0 12 242.0 13 268.0 14 378.0 15 499.0 16 608.0 17 745.0 18 910.0 19 1065.0 20 1379.0 21 1680.0 22 1987.0 23 2348.0 24 2683.0 25 3023.0 26 3354.0 27 3495.0 28 4121.0 29 4559.0 30 5023.0 31 6237.0 32 7211.0 33 8231.0 34 9322.0 35 10727.0 36 13018.0 37 15691.0 38 16612.0 39 13954.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.44052829716018 0.40429484993772047 0.10785835067185315 0.04731850223023235 8 99.00352801185745 0.7758842645104275 0.12803830015239342 0.09254942347971915 9 96.2277411677928 2.4542993730298455 0.7209112986841282 0.5970481604932257 10 45.76464612023074 37.0079397663301 7.0775953850543125 10.149818728384838 11 31.938597284753005 41.13926252722553 16.857216419520274 10.064923768501187 12 46.42919273243474 17.479315551782445 21.696229132888448 14.395262582894361 13 27.924165866288053 19.145752425495193 27.944349326985986 24.985732381230775 14 32.51960668332162 23.00610294987509 19.118168975859597 25.3561213909437 15 24.40366588550385 25.13949336011606 18.7004798571588 31.756360897221292 16 29.914779847940515 31.25226278998524 21.22148884618598 17.61146851588827 17 35.93561900255381 23.574356155232522 22.085910915960948 18.404113926252723 18 27.642932694984136 23.88660023381395 28.612286366419866 19.858180704782054 19 32.172108312903944 22.566581234677958 22.838895698685093 22.422414753733005 20 17.475241318791525 25.914086331536502 29.203404231610325 27.40726811806165 21 20.50561211353657 28.239403786871897 34.346969876206444 16.90801422338508 22 21.60785487137022 32.502940009881215 27.306950948805557 18.582254169943006 23 25.929147501513494 23.906281531171064 32.7450994036477 17.41947156366774 24 29.33399208110948 25.29800218500143 23.84991684469093 21.51808888919816 25 20.2876766664579 20.913968393144195 33.46137519745586 25.336979742942038 26 25.705835276105642 19.768259160026442 39.537214238491245 14.988691325376665 27 29.457445326647164 19.46998189162836 34.07090123972698 17.001671541997492 28 41.31887117111469 20.92973033222568 25.825687753836725 11.925710742822908 29 43.071729370410324 22.123690530052553 23.668952076010164 11.135628023526955 30 41.65019487750557 25.437778396436528 21.27435968819599 11.637667037861915 31 36.375912739017544 29.217393725506575 22.382553372174772 12.024140163301105 32 28.63689866761389 34.14335165103046 24.960822415846547 12.258927265509099 33 23.510882215609342 40.47570927793381 24.664264657713726 11.349143848743125 34 17.019173199292666 46.141689525056044 25.633885183586514 11.205252092064773 35 15.019110674826125 50.23218251564708 25.552608311229 9.196098498297792 36 14.121226044037899 50.90463560484236 24.89610091264123 10.078037438478514 37 12.807751289558064 51.72940192388123 25.461452669733724 10.00139411682699 38 13.38467870771506 51.94254340986305 25.541533245432284 9.131244636989598 39 12.38867695569395 51.37074670999867 25.93713604251372 10.30344029179365 40 10.48259909578383 37.66586058248344 23.605649598710265 28.245890723022466 41 8.948528585869932 33.19527125687971 22.33586993148377 35.52033022576659 42 12.412471694826685 50.68454973001219 26.665040933635254 10.237937641525868 43 11.567429599564836 42.91323207386641 25.869980612856885 19.649357713711872 44 11.02089627391742 44.219257021371824 25.188122412442652 19.5717242922681 45 12.554212163047874 51.12328996360289 26.526656347180968 9.795841526168264 46 12.489804454669038 50.59081878071735 26.456133012652934 10.463243751960682 47 12.671683455040341 48.65263729455416 25.811167343074537 12.86451190733096 48 13.243749956439618 51.31831139051708 25.826079077774448 9.611859575268856 49 11.164800738446047 39.84112221421928 23.081333958028853 25.912743089305817 50 12.406897753004703 51.85786448353945 25.890959763107475 9.844278000348371 51 12.550850236894071 51.86549719843978 25.49594244764955 10.0877101170166 52 12.705877434997074 51.302148649778445 25.588022183206533 10.403951732017948 53 12.478306022735985 51.599952604322766 25.381082154830526 10.540659218110724 54 11.428511650289078 47.18279899851451 24.67796941145292 16.71071993974349 55 13.049057761783208 50.6053758491303 25.87563292463489 10.469933464451604 56 13.2757058208435 50.73335657023353 26.019518996165914 9.971418612757057 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 5.0 5 10.0 6 15.0 7 20.0 8 158.5 9 297.0 10 560.0 11 823.0 12 1010.5 13 1129.0 14 1060.0 15 1141.5 16 1223.0 17 1262.0 18 1301.0 19 1372.0 20 1443.0 21 1690.5 22 2312.0 23 2686.0 24 3228.0 25 3770.0 26 4292.5 27 4815.0 28 5521.0 29 7142.5 30 8058.0 31 8917.0 32 9776.0 33 10546.0 34 11316.0 35 10850.0 36 10384.0 37 9393.5 38 7585.5 39 6768.0 40 6028.5 41 5289.0 42 4910.5 43 4532.0 44 4296.0 45 4060.0 46 3868.0 47 3698.0 48 3720.0 49 3828.0 50 3936.0 51 4250.0 52 4564.0 53 4887.5 54 5309.0 55 5407.0 56 5153.5 57 4900.0 58 4573.0 59 4246.0 60 3839.5 61 3433.0 62 3012.5 63 2343.0 64 2094.0 65 1784.0 66 1474.0 67 1272.5 68 1071.0 69 900.5 70 730.0 71 618.5 72 437.0 73 367.0 74 278.0 75 189.0 76 148.0 77 107.0 78 80.5 79 35.0 80 16.0 81 11.0 82 6.0 83 6.5 84 7.0 85 4.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.017396508172879542 14 0.004175161961491089 15 0.23032976820892512 16 0.05497296582629935 17 0.0 18 0.0020875809807455446 19 0.08559082021056734 20 0.08419909955673696 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0027834413076607265 26 0.008350323922982178 27 0.08837426151822807 28 0.11133765230642906 29 0.029226133730437625 30 0.018788228826709902 31 0.032009575038098356 32 0.0904618424989736 33 0.15030583061367922 34 0.04801436255714753 35 0.04871022288406271 36 0.04035989896108053 37 0.17187750074804986 38 0.2532931589971261 39 0.22128358395902772 40 0.7250864606456192 41 0.8760881515862136 42 0.12664657949856306 43 0.21780428232445184 44 0.49754013374435485 45 0.20110363447848745 46 0.18231540565177756 47 0.03896817830725017 48 0.1586561545366614 49 0.4898856701482879 50 0.12664657949856306 51 0.2741689688045815 52 0.12177555721015677 53 0.1628313164981525 54 0.22267530461285812 55 0.226154606247434 56 0.17883610401720165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 143707.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.26454522048334 #Duplication Level Percentage of deduplicated Percentage of total 1 88.45466053165624 61.267718343574074 2 6.440756294078644 8.922321111706458 3 2.037412847354779 4.233614228951964 4 0.9212562036609134 2.552415679124886 5 0.5636038497860114 1.9518882169970844 6 0.3325363177881814 1.3819786092535506 7 0.2129839860153911 1.0326567251421295 8 0.1587333480680745 0.8795674532207896 9 0.11452912455544616 0.7139526954149763 >10 0.6429705238200486 8.179142282561044 >50 0.07635274970363078 3.8063559882260436 >100 0.04420422351262834 5.078388665826996 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 326 0.22685046657434918 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAACAAAAAAAAAAAAAAA 290 0.20179949480540268 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 276 0.1920574502285901 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 258 0.17953196434411683 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAACAAAAAAAAAAAAAAA 245 0.1704857800942195 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAACCAAAAAAAAAAAAAAA 226 0.15726443388283104 No Hit ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 210 0.14613066865218813 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAACAAAAAAAAAAAAAAA 209 0.14543480832527295 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAA 207 0.14404308767144258 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAACAAAAAAAAAAAAAAA 204 0.14195550669069704 No Hit ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 201 0.1398679257099515 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAACAAAAAAAAAAAAAAA 198 0.13778034472920594 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 192 0.13360518276771485 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 191 0.1329093224407997 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 176 0.12247141753707197 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAACAAAAAAAAAAAAAAA 173 0.12038383655632642 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAACAAAAAAAAAAAAAAA 171 0.11899211590249606 No Hit ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 170 0.11829625557558086 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAACCAAAAAAAAAAAAAAA 168 0.1169045349217505 No Hit ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 164 0.11412109361408979 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAA 158 0.1099459316525987 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAACAAAAAAAAAAAAAAA 157 0.1092500713256835 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAACCAAAAAAAAAAAAAAA 153 0.10646663001802278 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAA 147 0.10229146805653169 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAAACAAAAAAAAAAAAAAA 146 0.10159560772961651 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0013917206538303633 0.0 0.0 0.0 0.0 9 0.0020875809807455446 0.0 0.0 0.0 0.0 10 0.022267530461285812 0.0 0.0 0.0 0.0 11 0.04244747994182608 0.0 0.0 0.0 0.0 12 0.050797803864808255 0.0 0.0 0.0 0.0 13 0.05497296582629935 0.0 0.0 0.0 0.0 14 0.06123570876853598 0.0 0.0 0.0 0.0 15 0.07445705497992443 0.0 0.0 0.0 0.0 16 0.08837426151822807 0.0 0.0 0.0 0.0 17 0.10089974740270133 0.0 0.0 0.0 0.0 18 0.11133765230642906 0.0 0.0 0.0 0.0 19 0.12177555721015677 0.0 0.0 0.0 0.0 20 0.1398679257099515 0.0 0.0 0.0 0.0 21 0.15796029420974622 0.0 0.0 0.0 0.0 22 0.18231540565177756 0.0 0.0 0.0 0.0 23 0.20319121545923302 0.0 0.0 0.0 0.0 24 0.2275463269012644 0.0 0.0 0.0 0.0 25 0.24633455572797427 0.0 0.0 0.0 0.0 26 0.26164348292010825 0.0 0.0 0.0 0.0 27 0.2804317117468182 0.0 0.0 0.0 0.0 28 0.29643649926586735 0.0 0.0 0.0 0.0 29 0.3096578454772558 0.0 0.0 0.0 0.0 30 0.31800816940023796 0.0 0.0 0.0 0.0 31 0.32218333136172905 0.0 0.0 0.0 0.0 32 0.3326212362654568 0.0 0.0 0.0 0.0 33 0.3402756998615238 0.0 0.0 0.0 0.0 34 0.34584258247684524 0.0 0.0 0.0 0.0 35 0.3597597890151489 0.0 0.0 0.0 0.0 36 0.37158941457270694 0.0 0.0 0.0 0.0 37 0.38759420209175616 0.0 0.0 0.0 0.0 38 0.3966403863416535 0.0 0.0 0.0 0.0 39 0.40290312928389016 0.0 0.0 0.0 0.0 40 0.409861732553042 0.0 0.0 0.0 0.0 41 0.42099549778368484 0.0 0.0 0.0 0.0 42 0.45230921249486805 0.0 0.0 0.0 0.0 43 0.4766643239368994 0.0 0.0 0.0 0.0 44 0.513544921263404 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGTCA 15 0.008130142 50.43889 39 AACTACA 15 0.008130142 50.43889 38 AAACTAC 20 4.0442465E-4 50.314827 37 ATGCTCG 15 0.00824398 50.26185 36 AAAACCT 15 0.00824398 50.26185 36 GCAAACC 15 0.0082668895 50.22659 35 CTCGGAC 15 0.008289846 50.19138 43 CCAACAC 85 0.0 50.191376 43 CAACACG 15 0.008312849 50.15622 44 CCGTCGC 15 0.008359 50.086044 49 TCATTAC 15 0.008405341 50.016068 50 GCGGATT 15 0.008405341 50.016068 50 ACGGGTA 20 4.179311E-4 49.981148 33 TGACGGG 20 4.2009607E-4 49.92887 31 TTGACGG 20 4.2009607E-4 49.92887 30 AACGCAC 15 0.0084635345 49.92887 30 GACGGGT 20 4.2009607E-4 49.92887 32 CGCACTG 15 0.0084635345 49.92887 32 GAAGCTA 15 0.00847521 49.91147 9 GAAGCAC 15 0.00847521 49.91147 9 >>END_MODULE