FastQCFastQC Report
Fri 17 Jun 2016
SRR1526240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68816
Sequences flagged as poor quality0
Sequence length56
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG30094.372529644268774No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG8181.1886770518484073No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG7781.1305510346431062No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG6690.9721576377586607No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG2150.31242734247849335No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1770.2572076261334573No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG1550.22523831667054175No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1420.20634736107881888No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG1060.1540339455940479No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC1050.15258079516391537No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1000.14531504301325274No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC970.14095559172285513No Hit
GTCGGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC930.13514299000232505No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC900.13078353871192747No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC780.11334573355033713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTC150.0080140250.59393338
TTGCCTG150.0080140250.59393338
CGTCTCA1150.050.51920341
ACGCGAC150.00808461750.4819237
TGCTAGT150.00808461750.4819237
AAGTCTT150.00808461750.4819237
CAGCATT150.00815567750.37039643
CCTCCAC150.00815567750.37039643
ATGCTCG1100.050.33333636
CGATTCA451.2369128E-1050.33333649
CGATTAA1250.050.33333649
TATGCCT1100.050.33333635
CACGCGA150.00817946550.33333236
AGATTCA150.00817946550.33333249
ACGATTA1300.050.29632648
AAGATTC150.00820330650.2963248
AACGATT1650.050.25936547
TGATATG150.00825114450.2224750
CAACGAT1550.050.2224746
TACGAGG150.00825114450.2224750