##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526240_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68816 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.763688677051846 38.0 38.0 39.0 35.0 40.0 2 37.49396942571495 38.0 38.0 39.0 35.0 40.0 3 37.580925947454084 38.0 38.0 39.0 35.0 40.0 4 37.38451813531737 38.0 38.0 39.0 35.0 40.0 5 37.31975122064636 38.0 38.0 39.0 35.0 40.0 6 36.38058009765171 38.0 35.0 39.0 32.0 40.0 7 37.8607445942804 39.0 38.0 39.0 35.0 40.0 8 37.67740060451058 39.0 38.0 39.0 35.0 40.0 9 36.867167519181585 39.0 35.0 39.0 32.0 40.0 10 36.46744943036503 38.0 35.0 39.0 32.0 40.0 11 34.888136479888395 38.0 33.0 39.0 27.0 40.0 12 33.58871483375959 38.0 32.0 39.0 21.0 40.0 13 33.477897581957684 37.0 32.0 39.0 21.0 40.0 14 33.47079167635434 38.0 32.0 39.0 20.0 40.0 15 33.43597419204836 38.0 32.0 39.0 20.0 40.0 16 33.67131190420832 38.0 32.0 39.0 22.0 40.0 17 33.561134038595675 37.0 31.0 39.0 22.0 40.0 18 33.160936410137175 37.0 31.0 39.0 20.0 40.0 19 33.82873169030458 37.0 31.0 39.0 24.0 40.0 20 33.82248314345501 37.0 32.0 39.0 24.0 40.0 21 34.669684375726575 38.0 33.0 39.0 26.0 40.0 22 34.43793594512904 38.0 33.0 39.0 25.0 40.0 23 34.89384736107882 38.0 33.0 39.0 27.0 40.0 24 34.38207684259474 38.0 33.0 39.0 25.0 40.0 25 34.61606312485468 38.0 33.0 39.0 26.0 40.0 26 35.13425656823994 38.0 35.0 39.0 28.0 40.0 27 35.074081608928154 38.0 35.0 39.0 27.0 40.0 28 34.71012555219716 38.0 34.0 39.0 27.0 40.0 29 34.66897233201581 38.0 34.0 39.0 27.0 40.0 30 34.27468902580795 38.0 33.0 39.0 25.0 40.0 31 34.65422285514997 38.0 34.0 39.0 27.0 40.0 32 34.34419321088119 38.0 33.0 39.0 26.0 40.0 33 34.47510753313183 38.0 33.0 39.0 27.0 40.0 34 34.35792548244594 38.0 33.0 39.0 27.0 40.0 35 34.11340385956754 38.0 33.0 39.0 25.0 40.0 36 33.80458033015578 37.0 33.0 38.0 26.0 39.0 37 33.51980644036271 36.0 32.0 38.0 25.0 39.0 38 33.263732271564756 36.0 32.0 38.0 24.0 39.0 39 32.688081260172055 36.0 31.0 38.0 21.0 39.0 40 29.380478377121598 35.0 23.0 38.0 9.0 39.0 41 26.296907695884677 31.0 15.0 38.0 5.0 39.0 42 30.41852185538247 34.0 28.0 38.0 10.0 39.0 43 28.820448732852824 33.0 25.0 38.0 5.0 39.0 44 29.016260753313183 33.0 26.0 38.0 4.0 39.0 45 30.03496279934899 34.0 28.0 38.0 4.0 39.0 46 29.595559172285515 35.0 27.0 38.0 4.0 39.0 47 29.136552545919553 34.0 26.0 38.0 4.0 39.0 48 29.162433155080215 34.0 26.0 38.0 4.0 39.0 49 26.84701232271565 33.0 16.0 38.0 4.0 39.0 50 28.757207626133457 34.0 25.0 38.0 4.0 39.0 51 28.974700651011393 35.0 26.0 38.0 4.0 39.0 52 28.821742036735643 35.0 25.0 38.0 4.0 39.0 53 28.33354161822832 34.0 23.0 38.0 4.0 39.0 54 27.45296152057661 33.0 18.0 38.0 4.0 39.0 55 27.70383341083469 34.0 22.0 38.0 4.0 39.0 56 26.498764822134387 33.0 17.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.11999066762523825 1 2 -0.21531531767761436 1 3 -0.28459177804752755 1 4 0.18218760897298836 1 5 0.23660170817563397 1 6 0.49485640116824925 1 7 0.2226216793970366 1 8 0.20914354839814564 1 9 0.37441218881310334 1 10 -0.13135628399775356 1 11 -4.636601051419515 1 12 -15.54538388008553 1 13 -11.954155818004466 1 14 -7.388937215048028 1 15 -6.91800853146626 1 16 -23.148469119696742 1 17 -5.473057163774939 1 18 -12.06045865609455 1 19 -5.641955285446798 1 20 -5.592931581074183 1 21 -4.97712498591083 1 22 -2.087365019262691 1 23 -3.0680232061694106 1 24 -3.4208810677924113 1 25 -3.016674447531461 1 26 2.2608233028485714 1 27 2.1070335995235894 1 28 2.026029857976283 1 29 2.6154260419889113 1 30 2.4237470574385185 1 31 1.4797565953940506 1 32 2.297463051301804 1 33 2.0888969810075864 1 34 2.003517272936435 1 35 1.1092160090657899 1 36 0.8225584163946635 1 37 0.7685823075885239 1 38 1.5804147734350238 1 39 1.8667134816202697 1 40 5.519365806678447 1 41 -9.23637777879621 1 42 -8.661744140850569 1 43 -16.72374783074016 1 44 -21.67356571501802 1 45 -10.751936513379388 1 46 -16.98743639449492 1 47 -22.752997559242218 1 48 -14.319007690104197 1 49 -19.941544792129935 1 50 -14.475452292744304 1 51 -11.632351517607788 1 52 -11.294883768363242 1 53 -10.818493551538712 1 54 -10.074454826683954 1 55 -10.307599711944825 1 56 -6.4755591420255385 2 1 0.765171934000783 2 2 1.0514529750053114 2 3 0.9961500918711721 2 4 0.8021063081599848 2 5 1.2404126837853866 2 6 1.385100303607274 2 7 1.042743630616549 2 8 1.2175276947396085 2 9 1.7466174733659514 2 10 1.6758083501485856 2 11 0.11111236321463025 2 12 -2.9821216849635803 2 13 -2.5407919968662576 2 14 2.510707093895064 2 15 2.796930492923984 2 16 2.1975674656691098 2 17 3.585377795574651 2 18 -2.151922070728695 2 19 2.137770324309301 2 20 2.3064586628282555 2 21 2.0955376157151875 2 22 2.593021159599097 2 23 1.3115499645622961 2 24 1.1891290948092177 2 25 1.8740775849888607 2 26 1.8604167987835254 2 27 2.1413323800113915 2 28 2.1660196953746578 2 29 2.4637492127206215 2 30 2.1181068135360803 2 31 1.4497769205973015 2 32 2.187706953740829 2 33 1.3856449484872613 2 34 2.0980294680583853 2 35 2.0007302367080655 2 36 2.607111261923123 2 37 2.9327083238486864 2 38 3.053737944166734 2 39 3.1436443759292167 2 40 6.636997920499585 2 41 -5.748064770666129 2 42 -6.4229230026391875 2 43 -12.314449050252353 2 44 -15.206340105261926 2 45 -6.219924318257437 2 46 -11.836775825389228 2 47 -17.57261138038043 2 48 -9.54639590148631 2 49 -13.595762271804732 2 50 -8.705635219573573 2 51 -4.817818997282583 2 52 -4.272271979745355 2 53 -4.146491519018387 2 54 -3.394576777903467 2 55 -3.7999777607253122 2 56 -0.8764737761718813 3 1 0.41553425284136125 3 2 -0.12517745977729788 3 3 0.36254501940739914 3 4 0.7839903661310004 3 5 0.8015539881332145 3 6 0.25466552099857864 3 7 0.917743630616549 3 8 0.9607342164787411 3 9 1.0695341400326157 3 10 0.9407540023224996 3 11 1.357036276258107 3 12 2.9023891846016383 3 13 1.3446246698004103 3 14 2.589058543170424 3 15 3.0822565798805073 3 16 3.8361544221908517 3 17 3.769254607168854 3 18 4.521990972749563 3 19 3.310324672135387 3 20 2.1379804019586928 3 21 2.4170955867296797 3 22 2.635593623367214 3 23 2.60774561673621 3 24 2.47943706582371 3 25 2.07425874440915 3 26 1.9654892625516425 3 27 1.322038901750524 3 28 1.9517986808819074 3 29 1.4216296475032308 3 30 1.5080524657099943 3 31 0.9325667756697698 3 32 0.9811852146103917 3 33 0.41100726732783954 3 34 0.7991164245801272 3 35 0.48487878743270585 3 36 0.9694301025028338 3 37 0.884701077471874 3 38 0.897487944166734 3 39 1.8619414773784904 3 40 4.875675456731468 3 41 4.767333780058507 3 42 2.3578740988100897 3 43 -4.573959919817572 3 44 -0.11259010526192625 3 45 1.6523582904382152 3 46 1.8810683775093224 3 47 -1.8778650035688358 3 48 1.7040569970644128 3 49 3.5568645397894727 3 50 2.5036039108612087 3 51 2.529101292572488 3 52 3.0433983101097155 3 53 3.000700509967121 3 54 3.7739014829660995 3 55 2.354913543622512 3 56 2.778417528175943 4 1 0.4957274895563373 4 2 0.23617519722753144 4 3 0.6135112029822807 4 4 0.8965507526044263 4 5 0.9897182393409452 4 6 1.2184336369406097 4 7 1.3066325195054347 4 8 1.15711102807294 4 9 1.9584230289215014 4 10 1.7209472390374714 4 11 3.1826401409924046 4 12 5.029683870591974 4 13 4.950180225355965 4 14 4.319734871672843 4 15 4.75109715959065 4 16 4.810067465669107 4 17 4.9228777955746565 4 18 4.975855707049085 4 19 4.296798102087081 4 20 3.8092364406060355 4 21 3.7830376157151875 4 22 3.7971878262657626 4 23 2.5490499645622933 4 24 3.817601317031439 4 25 2.864355362766638 4 26 2.4770834654501925 4 27 2.76286015778917 4 28 2.2208808064857735 4 29 2.7547214349428444 4 30 3.3215790357583046 4 31 0.6456102539306343 4 32 1.6543736204074975 4 33 1.5634227262650384 4 34 0.7008072458361596 4 35 0.8319802367080698 4 36 1.9848890397008958 4 37 2.281319434959798 4 38 2.28637683305562 4 39 3.745033264818101 4 40 5.8863034760551365 4 41 9.042213007111648 4 42 5.352076997360815 4 43 6.320967616414311 4 44 5.954076561404744 4 45 5.045353459520339 4 46 5.691696396832995 4 47 4.609333064064014 4 48 5.026520765180358 4 49 8.121598839306376 4 50 5.709642558204202 4 51 4.930792113828524 4 52 3.983978020254643 4 53 5.206980703203833 4 54 5.919312110985423 4 55 5.209744461496911 4 56 5.894359557161451 5 1 0.4664130687525514 5 2 0.3799104218138254 5 3 0.7499178223676211 5 4 0.7081346769543089 5 5 0.8554392795300672 5 6 1.0872279631817392 5 7 1.183701077425063 5 8 1.2088840777183307 5 9 1.5163426506709143 5 10 1.076739201212412 5 11 3.428266618533776 5 12 4.908170868227906 5 13 4.0627097761833895 5 14 5.049049292476624 5 15 4.995068790796324 5 16 4.818578103966985 5 17 5.271104745929264 5 18 4.587439631398965 5 19 4.195616068990155 5 20 3.7399693011261306 5 21 2.7369383249350463 5 22 3.3213013014430715 5 23 2.9937308156261224 5 24 3.4322585274333264 5 25 2.849698152364752 5 26 2.2203458767977153 5 27 2.92125259277735 5 28 1.6941668584952296 5 29 2.6239885744227465 5 30 2.8248887284296984 5 31 1.7711421688242552 5 32 1.6770686558684886 5 33 1.9778435300475437 5 34 1.6166464893349826 5 35 1.205739101956297 5 36 2.5503736732706415 5 37 2.6432579692387534 5 38 3.0783390079965187 5 39 3.8355770000426865 5 40 7.259116714825826 5 41 9.642449413731029 5 42 5.820871323601946 5 43 6.930896694428501 5 44 6.23847372452531 5 45 5.425022490253202 5 46 4.530467082412187 5 47 4.219498548697583 5 48 3.405953389293831 5 49 6.505523189188178 5 50 5.731864780426427 5 51 3.144503697752878 5 52 3.1109283748645744 5 53 3.771283303676647 5 54 4.336806200819936 5 55 3.2705009626789447 5 56 4.816345372764289 6 1 0.10459140965620861 6 2 -0.6669459013991812 6 3 -0.10509054483294733 6 4 -0.29246298022953 6 5 0.036409874796625274 6 6 -0.09242778628036774 6 7 0.4065076755603698 6 8 0.41029454867219073 6 9 0.4684105445269964 6 10 -0.021874234121078473 6 11 1.8958735991696898 6 12 3.4662631464970914 6 13 4.28563590575547 6 14 3.725477693145997 6 15 4.304655212025111 6 16 3.729168589264617 6 17 3.468570679469785 6 18 4.204819502305014 6 19 3.480818077118286 6 20 3.4820204605810616 6 21 2.7811649565391576 6 22 2.622281459224567 6 23 2.2209600769218483 6 24 2.2283378963073446 6 25 2.1097985587716366 6 26 2.0055478849258463 6 27 2.148003728325996 6 28 1.7532153882585533 6 29 0.8426115722711813 6 30 2.5791320944349536 6 31 1.609655197750861 6 32 2.5247856054262243 6 33 2.074783525266284 6 34 1.1668496927774896 6 35 1.4776731206031997 6 36 2.211980175780802 6 37 1.520021057931082 6 38 2.408723899222913 6 39 2.516069469562172 6 40 5.931122452334787 6 41 2.2854090121054043 6 42 3.6973953494207414 6 43 5.345312185702706 6 44 4.808758209344813 6 45 3.9338678165740255 6 46 3.6837063843485964 6 47 3.7768736383461636 6 48 3.254985184656011 6 49 5.604495218831971 6 50 4.4004041062691215 6 51 4.155261527061988 6 52 3.5656259603295517 6 53 3.987754735663259 6 54 5.269749064793164 6 55 3.1178593179263743 6 56 4.021700381131488 7 1 0.33944729631961934 7 2 0.4726486271792254 7 3 0.5799363237552271 7 4 0.2622512356962119 7 5 0.7906844229158239 7 6 1.2981437818681414 7 7 0.8742653697469862 7 8 1.1563863903917806 7 9 1.4064906617717483 7 10 0.9190148718877182 7 11 3.280949319736372 7 12 4.43499788025381 7 13 4.464189887191715 7 14 4.53471071708347 7 15 4.940952232054421 7 16 4.814415291756063 7 17 4.790993737603635 7 18 5.065469233619133 7 19 3.9081507590919102 7 20 3.3988499671760835 7 21 3.504052108468805 7 22 3.602984927715042 7 23 2.781658660214468 7 24 2.7837848919106634 7 25 2.780780483539587 7 26 2.2915762190733844 7 27 1.8872562930548682 7 28 2.4191899852297354 7 29 2.7151079083727936 7 30 2.8124002917969477 7 31 2.3021319930610744 7 32 2.3833591276538755 7 33 1.9001377021104489 7 34 1.8534642506670806 7 35 2.4305309613457524 7 36 2.9368214068506546 7 37 2.493396729645788 7 38 2.832270552862383 7 39 3.155419738248053 7 40 6.353936326296687 7 41 -0.9391879590719263 7 42 3.988308881418785 7 43 5.186909645399819 7 44 5.235235981694597 7 45 4.36974959478604 7 46 5.902807507944104 7 47 6.513439344257254 7 48 5.769274388368764 7 49 7.904690626745992 7 50 6.231864780426427 7 51 6.126927379529008 7 52 5.869485266631454 7 53 5.83765703170625 7 54 6.38259713514001 7 55 6.528826587100774 7 56 6.158852310784642 8 1 0.10683860066744444 8 2 0.19042356324060705 8 3 0.498478523243719 8 4 0.624655327767826 8 5 0.5165156249618548 8 6 0.5203944212543306 8 7 0.476567160028317 8 8 1.047307106504313 8 9 1.2754164929737897 8 10 1.2320583501485842 8 11 3.340156480861687 8 12 4.927069491507005 8 13 4.706389375682761 8 14 5.0321531723264314 8 15 5.272665787041632 8 16 4.080655700963227 8 17 4.415034658319755 8 18 4.303960282212479 8 19 3.9425497360740067 8 20 3.8491057216517817 8 21 2.843821929440672 8 22 3.7991486105794934 8 23 2.7255205527975903 8 24 3.5326339967700022 8 25 2.596381506557492 8 26 2.8908089556462713 8 27 2.6262588505996263 8 28 2.1607500875315253 8 29 2.8501462715441477 8 30 3.246415637065489 8 31 1.6809043715776966 8 32 2.9994716596231825 8 33 2.29937043868334 8 34 2.0818529974701505 8 35 2.471195922982581 8 36 1.4326014580015496 8 37 2.2133455787506477 8 38 1.3033702971079109 8 39 2.7816345720076434 8 40 4.920943998930955 8 41 -3.2022314373327987 8 42 3.983449546380424 8 43 5.001359773277056 8 44 4.510939306502781 8 45 4.214634505271977 8 46 3.391042802061751 8 47 3.8158690117764316 8 48 3.75201096125879 8 49 6.6379387085874235 8 50 4.9730412510146635 8 51 4.058896688991929 8 52 4.1820172359409185 8 53 4.753385931962008 8 54 4.71865851621418 8 55 4.752228121627628 8 56 4.455143870886943 9 1 -0.11623832240947252 9 2 -0.24502138396904627 9 3 0.1331693226403985 9 4 -0.20430394825027065 9 5 0.1418549914776932 9 6 0.372279790786763 9 7 0.36646157933449786 9 8 0.11095718191909754 9 9 0.9669700374685135 9 10 -0.001274983184750056 9 11 2.4228110811633456 9 12 4.161307802215909 9 13 3.5818041569798993 9 14 3.2855468374848087 9 15 4.548533057026546 9 16 3.099811055412701 9 17 3.3305701032669575 9 18 3.1237189549123343 9 19 2.7395331448221185 9 20 2.649407380776971 9 21 2.116370949048516 9 22 2.4535980826760238 9 23 1.697767913280245 9 24 2.672302171732291 9 25 1.7506801490914299 9 26 1.43092961929635 9 27 1.779954174883187 9 28 1.9584876440926067 9 29 1.4068581870795924 9 30 1.9378183519976204 9 31 1.0250974334178196 9 32 1.264630030663909 9 33 0.7959013587436701 9 34 1.120465365494283 9 35 1.4422366469644814 9 36 0.671213826025685 9 37 0.1137980674384309 9 38 0.8077443544231429 9 39 0.6997341195189613 9 40 3.461517151268815 9 41 1.0131531780518195 9 42 1.7725898178736337 9 43 2.4619932574399535 9 44 2.5540765614047416 9 45 1.5573192714861541 9 46 3.518192123328717 9 47 3.4802732350041836 9 48 3.423956662616252 9 49 6.0412569589644995 9 50 4.385710934272581 9 51 3.029937412973826 9 52 2.8070549433315684 9 53 3.42322001944315 9 54 3.84580783748115 9 55 4.131111982864432 9 56 3.830256993058889 10 1 0.39879262365595025 10 2 0.48751619339611807 10 3 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118 24 -0.5197724203422993 118 25 -0.10736180895052883 118 26 0.9346592230259532 118 27 0.9578096527386677 118 28 1.8067393923443547 118 29 0.059771939993346734 118 30 1.8720840862633565 118 31 -0.5362079278875456 118 32 -5.685020318986442 118 33 0.4704934333357471 118 34 -0.03151598648707221 118 35 0.6198590245868587 118 36 1.628323383135239 118 37 1.6186931723335292 118 38 2.033851580530367 118 39 2.268265588050429 118 40 5.661050950802615 118 41 3.415950380849022 118 42 -1.653983608699793 118 43 -8.736608141161444 118 44 -5.839862832534653 118 45 -1.4869697728028903 118 46 -7.66991976478317 118 47 -8.829050774319825 118 48 -10.87044893178934 118 49 -8.056178938471398 118 50 -8.059044310482662 118 51 -7.028803845767431 118 52 -6.728143191866568 118 53 -7.663726367503237 118 54 -11.269576777903467 118 55 -5.739750487998041 118 56 -3.148064685262792 119 1 -0.2524834332308572 119 2 -0.01316566906248795 119 3 0.00974472463953191 119 4 -0.09478634720724699 119 5 0.15619658209047316 119 6 0.34272742225134323 119 7 -0.005985182942772838 119 8 0.20004888118028674 119 9 -0.24402518200128043 119 10 -0.6425179210378573 119 11 -5.888357975768422 119 12 -11.130956430726293 119 13 -11.820629567487725 119 14 -13.876122002150137 119 15 -14.752857642669237 119 16 -17.600102025856316 119 17 -17.355653277871674 119 18 -17.48666783344056 119 19 -7.487759336707647 119 20 -10.136337947341236 119 21 -1.2706347006689924 119 22 0.05876974716971972 119 23 0.27447369337585314 119 24 0.4823847445267262 119 25 0.6131312573052483 119 26 1.8844280982185566 119 27 1.0613535664520697 119 28 1.5041199778605332 119 29 1.1189398906867254 119 30 1.2298652881123502 119 31 0.3710339827441942 119 32 -6.947886266598154 119 33 -0.47311211365963146 119 34 -0.14985188787381531 119 35 -0.8889237180941905 119 36 0.7059813184202923 119 37 0.20636651593908084 119 38 -0.6703086660027573 119 39 0.3939974832738464 119 40 3.8465671295391317 119 41 0.12658212198923735 119 42 -4.177866505464046 119 43 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120 25 -3.445205360747572 120 26 2.2111919925819805 120 27 1.2292684265230207 120 28 1.77333558684753 120 29 1.6766852592322508 120 30 2.236566115861663 120 31 0.4037497888143591 120 32 -11.5564790927708 120 33 1.1685906849213694 120 34 1.1532620261979218 120 35 0.17926705841349388 120 36 1.2621500216130457 120 37 0.5950145253990726 120 38 0.4672553860271975 120 39 -0.06323546903202626 120 40 3.967182029026713 120 41 -1.8347895768676814 120 42 -4.892884242949265 120 43 -15.287559515368633 120 44 -9.763752895959602 120 45 -9.078935946164414 120 46 -16.620448306009386 120 47 -14.859917581930818 120 48 -12.818440475129721 120 49 -18.693388240796978 120 50 -11.651856149806132 120 51 -9.16326473371669 120 52 -9.96873515803993 120 53 -11.258652371731568 120 54 -9.897483754647652 120 55 -10.170617295609034 120 56 -5.299438892450951 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 123.0 5 25.0 6 67.0 7 74.0 8 142.0 9 232.0 10 112.0 11 73.0 12 96.0 13 118.0 14 131.0 15 148.0 16 235.0 17 258.0 18 353.0 19 415.0 20 541.0 21 678.0 22 844.0 23 998.0 24 1217.0 25 1395.0 26 1513.0 27 1638.0 28 2027.0 29 2429.0 30 2868.0 31 3356.0 32 3842.0 33 4429.0 34 5172.0 35 6292.0 36 7995.0 37 8940.0 38 6844.0 39 3196.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.31701929783772 0.5754475703324808 0.079923273657289 0.027609858172518018 8 99.3431760055801 0.5100558009765171 0.0900953266682167 0.056672866775168564 9 97.56161357823761 1.6347942338990933 0.5158684026970473 0.2877237851662404 10 45.764066496163686 26.974831434550104 20.255463845617296 7.005638223668914 11 30.751569402464547 40.202859800046504 15.637351778656129 13.40821901883283 12 30.447860962566846 14.23070216228784 19.783189955824227 35.53824691932109 13 22.594476744186046 13.453488372093023 23.38953488372093 40.5625 14 21.422654944416188 16.715832304003488 37.24769309016929 24.61381966141103 15 36.493746633082424 14.974593421953031 23.809385146251618 24.722274798712927 16 39.45715697961795 17.2840403570494 21.472392638036812 21.786410025295847 17 27.060567309927926 17.20966054405952 32.90804464078121 22.821727505231344 18 24.405661474075796 18.892408742152988 34.64601255521972 22.0559172285515 19 39.47552142420805 20.5820752247142 18.48765162754167 21.454751723536088 20 26.022456222002443 32.91785444179417 19.211414276572224 21.84827505963116 21 26.070971867007675 19.004301325273193 33.45297605208091 21.471750755638226 22 25.26592652871425 18.162927226226458 21.11282259939549 35.458323645663796 23 25.167112299465238 18.510230179028135 21.10846314810509 35.21419437340153 24 24.87357591257847 18.821204371076494 34.42222738897931 21.88299232736573 25 38.17862125087189 18.334398976982097 21.94693094629156 21.540048825854452 26 23.01834142371305 18.178647210999042 23.917972269860186 34.88503909542772 27 22.97409341498538 16.53744890686139 25.270920912912565 35.217536765240666 28 23.31374032473957 29.798347203631497 25.817668625967528 21.07024384566141 29 35.866499985463854 18.43620083146786 26.134546617437564 19.562752565630724 30 23.04360465116279 17.107558139534884 40.85319767441861 18.995639534883722 31 21.8935341318758 29.50052331666473 29.25049424351669 19.355448307942787 32 35.382064632479135 17.151458739346694 29.013351173681606 18.453125454492568 33 22.60445479691367 16.7025767942932 30.387247051972633 30.3057213568205 34 20.395195998604166 29.511748284285215 33.23106897755031 16.86198673956031 35 19.86565276182445 18.229931517803916 46.49955653779607 15.40485918257557 36 20.560272140489623 31.01994533930337 31.852939466185966 16.56684305402105 37 19.95660973514466 18.507840824706243 44.47647752588127 17.059071914267825 38 33.39600367277338 18.881261568507426 31.80738344045589 15.915351318263301 39 21.79565571861169 17.308700209643607 30.948637316561843 29.947006755182855 40 16.735452523123755 13.664968973188152 38.711801896733405 30.887776606954688 41 14.856941838649156 13.203564727954973 37.28160178236398 34.657891651031896 42 30.287992781989903 16.815344092437098 33.39057292955164 19.506090196021365 43 16.36400704830418 14.352910337998223 44.7159562539137 24.56712635978389 44 16.612396875228153 15.098196685405563 32.12966342994817 36.15974300941812 45 18.763652249890782 16.91859618465123 45.412844036697244 18.904907528760738 46 18.227074235807862 17.832605531295485 33.45269286754003 30.487627365356623 47 17.72341415198558 28.279260422117563 33.823187394615964 20.17413803128089 48 19.083725059304644 29.99286888943868 33.63410126176997 17.289304789486707 49 15.766265623174863 23.44498306272632 30.39218549234902 30.396565821749792 50 30.03637951105937 17.673166472642606 34.300058207217695 17.990395809080326 51 19.137026239067055 30.06413994169096 33.555393586005835 17.24344023323615 52 19.491365137561285 18.045189355913465 44.89837487087716 17.565070635648087 53 18.517979327413016 18.311253457562966 45.08370941912942 18.087057795894598 54 18.539178561025345 27.663850859306727 31.52636178269735 22.270608796970578 55 18.17228420776648 30.55175226498092 33.41101756634719 17.86494596090541 56 30.569103323773057 18.65709668496222 32.04682108695969 18.726978904305035 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.5 11 3.0 12 6.5 13 32.0 14 54.0 15 95.5 16 137.0 17 149.5 18 162.0 19 248.0 20 334.0 21 386.5 22 475.0 23 511.0 24 652.5 25 794.0 26 1003.0 27 1212.0 28 1379.5 29 1731.0 30 1915.0 31 1977.5 32 2040.0 33 2071.5 34 2103.0 35 1891.5 36 1680.0 37 1495.0 38 1180.0 39 1050.0 40 938.5 41 827.0 42 888.0 43 949.0 44 1038.5 45 1128.0 46 1381.0 47 3377.5 48 5121.0 49 4916.5 50 4712.0 51 3581.5 52 2451.0 53 2310.0 54 2373.0 55 2577.0 56 2590.0 57 2603.0 58 2758.5 59 2914.0 60 3202.5 61 3491.0 62 3893.5 63 4217.5 64 4139.0 65 3848.0 66 3557.0 67 3263.0 68 2969.0 69 2683.0 70 2397.0 71 2126.5 72 1608.0 73 1360.0 74 1200.5 75 1041.0 76 836.5 77 632.0 78 483.0 79 260.5 80 187.0 81 144.0 82 101.0 83 76.5 84 52.0 85 30.5 86 9.0 87 7.0 88 4.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.023250406882120437 14 0.0014531504301325273 15 0.19326900720762613 16 0.04359451290397582 17 0.0 18 0.0 19 0.0900953266682167 20 0.08718902580795164 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.014531504301325274 27 0.1002673796791444 28 0.1220646361311323 29 0.0319693094629156 30 0.023250406882120437 31 0.03487561032318066 32 0.08428272494768659 33 0.18309695419669844 34 0.0581260172053011 35 0.056672866775168564 36 0.040688212043710766 37 0.19908160892815624 38 0.294989537316903 39 0.1860032550569635 40 0.7091374099046733 41 0.8602650546384563 42 0.1438618925831202 43 0.2136131132294815 44 0.48099279237386655 45 0.21215996279934898 46 0.16856544989537317 47 0.02906300860265055 48 0.14967449430365032 49 0.47663334108346894 50 0.13950244129272263 51 0.3138804929086259 52 0.12061148570099976 53 0.18309695419669844 54 0.22669146710067425 55 0.23541036968146942 56 0.18745640548709602 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 68816.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.6150895140665 #Duplication Level Percentage of deduplicated Percentage of total 1 86.24113475177305 58.31202046035806 2 7.825059101654847 10.581841432225064 3 2.6520524392864817 5.379562892350616 4 1.160541586073501 3.1388049290862594 5 0.6576402321083172 2.2233201581027666 6 0.3202235117128734 1.2991164845384795 7 0.2793896410917688 1.3223668914206 8 0.17623038899634644 0.9532666821669379 9 0.1289490651192779 0.7847012322715649 >10 0.5136471093917903 5.866368286445013 >50 0.02364066193853428 1.1668797953964194 >100 0.01289490651192779 1.3078353871192745 >500 0.006447453255963895 3.2913857242501745 >1k 0.002149151085321298 4.372529644268774 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 3009 4.372529644268774 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 818 1.1886770518484073 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 778 1.1305510346431062 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 669 0.9721576377586607 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 215 0.31242734247849335 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 177 0.2572076261334573 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 155 0.22523831667054175 No Hit GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 142 0.20634736107881888 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG 106 0.1540339455940479 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 105 0.15258079516391537 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 100 0.14531504301325274 No Hit GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 97 0.14095559172285513 No Hit GTCGGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 93 0.13514299000232505 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 90 0.13078353871192747 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 78 0.11334573355033713 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0014531504301325273 0.0 0.0 0.0 0.0 10 0.0014531504301325273 0.0 0.0 0.0 0.0 11 0.004359451290397582 0.0 0.0 0.0 0.0 12 0.005812601720530109 0.0 0.0 0.0 0.0 13 0.005812601720530109 0.0 0.0 0.0 0.0 14 0.005812601720530109 0.0 0.0 0.0 0.0 15 0.013078353871192746 0.0 0.0 0.0 0.0 16 0.0159846547314578 0.0 0.0 0.0 0.0 17 0.018890955591722856 0.0 0.0 0.0 0.0 18 0.024703557312252964 0.0 0.0 0.0 0.0 19 0.024703557312252964 0.0 0.0 0.0 0.0 20 0.030516159032783072 0.0 0.0 0.0 0.0 21 0.03342245989304813 0.0 0.0 0.0 0.0 22 0.03923506161357824 0.0 0.0 0.0 0.0 23 0.04359451290397582 0.0 0.0 0.0 0.0 24 0.0479539641943734 0.0 0.0 0.0 0.0 25 0.05376656591490351 0.0 0.0 0.0 0.0 26 0.056672866775168564 0.0 0.0 0.0 0.0 27 0.061032318065566145 0.0 0.0 0.0 0.0 28 0.06539176935596373 0.0 0.0 0.0 0.0 29 0.06539176935596373 0.0 0.0 0.0 0.0 30 0.06539176935596373 0.0 0.0 0.0 0.0 31 0.06539176935596373 0.0 0.0 0.0 0.0 32 0.06539176935596373 0.0 0.0 0.0 0.0 33 0.06539176935596373 0.0 0.0 0.0 0.0 34 0.06684491978609626 0.0 0.0 0.0 0.0 35 0.06829807021622879 0.0 0.0 0.0 0.0 36 0.06975122064636131 0.0 0.0 0.0 0.0 37 0.07120437107649384 0.0 0.0 0.0 0.0 38 0.0741106719367589 0.0 0.0 0.0 0.0 39 0.0741106719367589 0.0 0.0 0.0 0.0 40 0.0741106719367589 0.0 0.0 0.0 0.0 41 0.07556382236689142 0.0 0.0 0.0 0.0 42 0.07701697279702395 0.0 0.0 0.0 0.0 43 0.08137642408742153 0.0 0.0 0.0 0.0 44 0.08282957451755406 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTAGTC 15 0.00801402 50.593933 38 TTGCCTG 15 0.00801402 50.593933 38 CGTCTCA 115 0.0 50.519203 41 ACGCGAC 15 0.008084617 50.48192 37 TGCTAGT 15 0.008084617 50.48192 37 AAGTCTT 15 0.008084617 50.48192 37 CAGCATT 15 0.008155677 50.370396 43 CCTCCAC 15 0.008155677 50.370396 43 ATGCTCG 110 0.0 50.333336 36 CGATTCA 45 1.2369128E-10 50.333336 49 CGATTAA 125 0.0 50.333336 49 TATGCCT 110 0.0 50.333336 35 CACGCGA 15 0.008179465 50.333332 36 AGATTCA 15 0.008179465 50.333332 49 ACGATTA 130 0.0 50.296326 48 AAGATTC 15 0.008203306 50.29632 48 AACGATT 165 0.0 50.259365 47 TGATATG 15 0.008251144 50.22247 50 CAACGAT 155 0.0 50.22247 46 TACGAGG 15 0.008251144 50.22247 50 >>END_MODULE