FastQCFastQC Report
Fri 17 Jun 2016
SRR1526239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28550
Sequences flagged as poor quality0
Sequence length56
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1130.3957968476357268No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC810.28371278458844135No Hit
CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG640.22416812609457093No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC580.2031523642732049No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC550.1926444833625219No Hit
CTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT320.11208406304728546No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC290.10157618213660245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACG150.00824305950.1587313
GAGTGGT150.00830082150.07042311
GTACCTG150.00830082150.0704239
CTGACGA150.00830082150.07042314
GAGCAGT150.00830082150.0704239
GGCCCGG150.00830082150.0704239
TACCTGG150.00830082150.07042310
TCATCTC150.00830082150.07042315
CGACTGT150.00830082150.07042346
GTACGCC150.00830082150.0704239
AAGCATG204.0863338E-450.0704210
TAAGCTC150.00835888549.98242649
GGATGGC150.00841724949.8947378
CCAGTAG150.00841724949.89473726
GGGTACC204.1580698E-449.8947377
GGACAGT150.00841724949.8947378
GCCAGTA150.00841724949.89473725
TGCCAGT150.00841724949.89473724
CGGTTTC150.00841724949.89473719
TCTTCCA150.00841724949.89473750
Produced by FastQC (version 0.11.5)