##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526239_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28550 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.25008756567426 38.0 38.0 40.0 36.0 40.0 2 37.839194395796845 38.0 38.0 40.0 35.0 40.0 3 37.31338003502627 38.0 38.0 40.0 33.0 40.0 4 37.39824868651488 38.0 38.0 40.0 34.0 40.0 5 37.38802101576182 38.0 38.0 40.0 33.0 40.0 6 37.19054290718039 39.0 36.0 40.0 33.0 40.0 7 38.06259194395797 39.0 38.0 40.0 35.0 40.0 8 37.87022767075307 39.0 38.0 40.0 35.0 40.0 9 37.89292469352014 39.0 38.0 40.0 35.0 40.0 10 37.00577933450088 39.0 35.0 40.0 32.0 40.0 11 35.34045534150613 38.0 33.0 39.0 28.0 40.0 12 33.85768826619965 38.0 32.0 39.0 21.0 40.0 13 33.63397548161121 38.0 32.0 39.0 20.0 40.0 14 33.899019264448334 38.0 32.0 39.0 21.0 40.0 15 34.05345008756567 38.0 33.0 39.0 21.0 40.0 16 34.0061295971979 38.0 33.0 39.0 22.0 40.0 17 33.934816112084064 38.0 32.0 39.0 22.0 40.0 18 33.37040280210157 37.0 31.0 39.0 20.0 40.0 19 34.40028021015762 38.0 33.0 39.0 25.0 40.0 20 34.270507880910685 38.0 32.0 39.0 24.0 40.0 21 35.180735551663744 38.0 34.0 39.0 27.0 40.0 22 34.771313485113836 38.0 33.0 39.0 25.0 40.0 23 35.320385288966726 38.0 35.0 39.0 27.0 40.0 24 34.843852889667254 38.0 33.0 39.0 25.0 40.0 25 35.08679509632224 38.0 34.0 39.0 27.0 40.0 26 35.775201401050786 38.0 35.0 39.0 29.0 40.0 27 35.479509632224165 38.0 35.0 39.0 28.0 40.0 28 35.61411558669002 38.0 35.0 39.0 29.0 40.0 29 35.39768826619965 38.0 35.0 39.0 28.0 40.0 30 35.27810858143608 38.0 35.0 39.0 27.0 40.0 31 35.36490367775832 38.0 35.0 39.0 28.0 40.0 32 35.01733800350263 38.0 35.0 39.0 27.0 40.0 33 35.26028021015762 38.0 35.0 39.0 28.0 40.0 34 35.101330998248685 38.0 35.0 39.0 28.0 40.0 35 34.94819614711033 38.0 35.0 39.0 27.0 40.0 36 34.38493870402802 37.0 33.0 39.0 27.0 40.0 37 34.16171628721541 37.0 33.0 39.0 27.0 40.0 38 33.89292469352014 37.0 33.0 39.0 26.0 40.0 39 33.39709281961471 36.0 32.0 38.0 23.0 40.0 40 30.062241681260947 35.0 26.0 38.0 9.0 40.0 41 26.960805604203152 32.0 16.0 38.0 6.0 39.0 42 30.88602451838879 35.0 29.0 38.0 12.0 40.0 43 29.398528896672506 34.0 26.0 38.0 6.0 39.0 44 29.4015761821366 34.0 26.0 38.0 5.0 39.0 45 30.491278458844132 35.0 29.0 38.0 4.0 39.0 46 30.055446584938704 35.0 27.0 38.0 4.0 40.0 47 29.555306479859894 35.0 27.0 38.0 4.0 40.0 48 29.529702276707532 35.0 27.0 38.0 4.0 40.0 49 26.99089316987741 33.0 16.0 38.0 4.0 40.0 50 29.158879159369526 34.0 26.0 38.0 4.0 40.0 51 29.48690017513135 35.0 27.0 39.0 4.0 40.0 52 29.188686514886165 35.0 26.0 39.0 4.0 40.0 53 28.783817863397548 35.0 25.0 39.0 4.0 40.0 54 27.770998248686514 34.0 19.0 38.0 4.0 40.0 55 28.17919439579685 34.0 23.0 38.0 4.0 40.0 56 27.187740805604204 33.0 17.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.06759039859862526 1 2 0.6528178865364112 1 3 0.8144441000709932 1 4 0.4926692308712717 1 5 0.9676305909943679 1 6 1.4734931958175608 1 7 0.4486901426022456 1 8 0.5282405900895881 1 9 0.41295225844712746 1 10 0.6696858483111257 1 11 -5.510511930690864 1 12 -13.542490289648544 1 13 -10.49929204637354 1 14 -5.971620325939586 1 15 -6.096317227543949 1 16 -23.574568700054257 1 17 -3.5265580080183767 1 18 -11.900544145902806 1 19 -4.202077927765654 1 20 -3.6333029160337666 1 21 -3.723376494603439 1 22 -1.8953633929840095 1 23 -1.542743899943062 1 24 -1.1562171079375503 1 25 -2.8310571865930108 1 26 1.2112622065022052 1 27 2.355555982442837 1 28 2.817947488325707 1 29 2.656284999895128 1 30 2.2957881818304102 1 31 2.0491344259718858 1 32 2.2416956094171283 1 33 2.612766475293256 1 34 1.4873875906516076 1 35 2.4481879681282237 1 36 2.938222638213908 1 37 3.3615285488752846 1 38 3.562357512070413 1 39 4.668394767940285 1 40 8.221640480741833 1 41 -7.608127585029276 1 42 -6.347354506897819 1 43 -16.58182201739713 1 44 -20.57428893499383 1 45 -8.1654027719959 1 46 -16.833959638080195 1 47 -22.328336264351268 1 48 -12.259910023565777 1 49 -19.696521116621458 1 50 -11.645588481771274 1 51 -7.425957772704756 1 52 -7.744584920876644 1 53 -7.140762256172177 1 54 -6.559370585849237 1 55 -6.740923488727695 1 56 -1.8515601275731157 2 1 0.7742106076578565 2 2 0.6960234614493004 2 3 1.0262907899664597 2 4 0.904515920766741 2 5 0.9139720544090011 2 6 1.6957928473855048 2 7 0.9351012924280298 2 8 1.1289374542010862 2 9 1.0707919797014824 2 10 1.1435534441299424 2 11 0.07764137941367011 2 12 -3.9027690352931437 2 13 -0.5418007571749328 2 14 2.9754180015865472 2 15 2.6918360825605845 2 16 2.9961630072628154 2 17 2.748006451911941 2 18 -1.2810319507808572 2 19 2.873880260387658 2 20 2.379937502084701 2 21 3.0139057353617176 2 22 2.4906993247860214 2 23 2.460740420614428 2 24 2.1615529269056566 2 25 2.3780020468564658 2 26 2.823805760509174 2 27 3.0579950068330817 2 28 2.615160031879711 2 29 2.854194407560634 2 30 2.410073896116124 2 31 2.483977631546793 2 32 3.2019743550617292 2 33 3.175832677383845 2 34 2.868572259641155 2 35 2.956898769521956 2 36 3.776550164346311 2 37 3.638183601140092 2 38 4.168629289073898 2 39 4.378499297557006 2 40 6.510839087013615 2 41 -6.8708453550641195 2 42 -4.169654158465761 2 43 -14.267536303111413 2 44 -13.625160015133202 2 45 -3.6093052110202883 2 46 -6.6276878610767085 2 47 -15.43147215285301 2 48 -6.505206190813162 2 49 -10.00662564623818 2 50 -4.554299283165005 2 51 1.4806624363544785 2 52 1.628934242886423 2 53 2.1101088239671952 2 54 3.1730335953354327 2 55 2.5872995077879857 2 56 4.376314436886815 3 1 0.8192387502469742 3 2 1.1275431612616842 3 3 1.826165711792605 3 4 1.653108791310835 3 5 1.280451103814876 3 6 1.8771561994805595 3 7 0.49044838436049076 3 8 1.120181982030978 3 9 1.1133185588134253 3 10 1.4718836505089286 3 11 2.713663893484963 3 12 4.393040845882588 3 13 3.479462532381042 3 14 4.794680040360781 3 15 5.327858596631877 3 16 5.210046684561128 3 17 4.990658109197739 3 18 5.166122520763857 3 19 4.32979020410248 3 20 3.931532248801396 3 21 3.8531802819533354 3 22 3.9735010758804563 3 23 3.288757931558763 3 24 3.707519155798714 3 25 2.366745011209183 3 26 2.4097970050369995 3 27 1.437607264494119 3 28 2.932233202611421 3 29 2.6240505676606958 3 30 3.333150819193044 3 31 1.661588638426096 3 32 2.418984986706505 3 33 1.9732060357328152 3 34 2.1049700082340266 3 35 2.308993828934085 3 36 3.37192227191354 3 37 3.213543200889937 3 38 3.2949582446711503 3 39 3.841288540834057 3 40 8.313215572316928 3 41 8.968429191527498 3 42 5.879751720208407 3 43 0.7581047225296125 3 44 0.4498868891819967 3 45 4.608956202363071 3 46 2.5755641714436166 3 47 -0.7510468870618894 3 48 3.217745654089903 3 49 5.299815879715538 3 50 3.4782210420382498 3 51 5.9359469892000085 3 52 5.936001159709438 3 53 5.650446535036618 3 54 5.740263113784465 3 55 4.899369551565346 3 56 6.026095550082562 4 1 0.5152094462176748 4 2 0.4718655055840273 4 3 1.1430155286424224 4 4 0.4021930403950833 4 5 0.8390591624229344 4 6 1.0639693862937492 4 7 0.8677377616498632 4 8 0.7996691615181604 4 9 0.5034284489233158 4 10 0.883971562596841 4 11 2.937107116928182 4 12 3.733700186541931 4 13 4.448327001245509 4 14 4.261713007393745 4 15 4.265587534360812 4 16 4.444478918993362 4 17 4.111537230076863 4 18 5.642312996954338 4 19 3.6503030246152974 4 20 3.7190780363471845 4 21 3.2670996958727514 4 22 3.7189223213017044 4 23 3.1953513381521716 4 24 3.1104495587291154 4 25 2.8117999562641316 4 26 2.4445955398355395 4 27 1.6079369348237833 4 28 1.7179474883257058 4 29 2.7229516665617908 4 30 1.4338834199256496 4 31 1.5205629974004538 4 32 1.7797908475123663 4 33 1.9365759991027787 4 34 1.0445304477944646 4 35 1.7815213014615594 4 36 2.476317876309146 4 37 2.2758142631609957 4 38 1.4195003692132744 4 39 2.949347148892663 4 40 6.0549738140751685 4 41 9.239491462589768 4 42 6.247883588340276 4 43 6.708654173079061 4 44 6.792377731672836 4 45 4.3965019899088595 4 46 5.123183219062664 4 47 4.585949449934446 4 48 4.421042357386607 4 49 4.922526502426162 4 50 4.549649613466823 4 51 3.4597565130095305 4 52 3.2601769838852626 4 53 3.5259044104944905 4 54 3.3025341760555236 4 55 4.406695558891354 4 56 2.1103446343316463 5 1 0.5211618271700544 5 2 0.5563893151078361 5 3 0.5037298143567099 5 4 0.956954945156987 5 5 0.5926305909943679 5 6 1.6842074815318426 5 7 0.6808329997451068 5 8 0.7996691615181604 5 9 0.19152368701855949 5 10 1.0161144197396936 5 11 2.7002023550234213 5 12 5.153938281780029 5 13 5.868565096483607 5 14 4.574808245488988 5 15 5.064397058170336 5 16 5.10043129994574 5 17 4.366299134838769 5 18 5.599455854097194 5 19 4.508636357948632 5 20 4.073839941109092 5 21 2.7980520768251296 5 22 3.17606517844456 5 23 3.7143989571997906 5 24 3.9652114634910234 5 25 3.3975142419784135 5 26 1.9969764922164899 5 27 2.180555982442833 5 28 1.807233202611421 5 29 0.7669992856094083 5 30 1.785073896116124 5 31 0.4455629974004509 5 32 1.295267037988559 5 33 1.1770521895789656 5 34 0.8838161620801799 5 35 1.3410451109853696 5 36 2.341794066785333 5 37 1.6686714060181416 5 38 2.5552146549275605 5 39 2.929109053654571 5 40 7.33949762359898 5 41 7.888300986399294 5 42 5.6669312073878935 5 43 6.532463696888584 5 44 5.536425350720457 5 45 4.459597228004103 5 46 5.083897504776946 5 47 4.750235164220165 5 48 4.54723283357708 5 49 7.4427645976642545 5 50 4.636554375371585 5 51 5.727613655866673 5 52 5.491129364837644 5 53 6.748523458113535 5 54 6.162057985579331 5 55 4.47693365412945 5 56 4.48058272956974 6 1 0.7908986692753146 6 2 0.9129682624762552 6 3 0.7708350775146044 6 4 1.4122181030517282 6 5 1.5307884857312075 6 6 1.659207481531844 6 7 0.8163593155345765 6 8 1.0628270562550028 6 9 1.0046815817553991 6 10 0.6358512618449552 6 11 3.096254986602368 6 12 4.960517229148444 6 13 4.017249307009919 6 14 5.02612403496267 6 15 5.342028637117707 6 16 5.272799720998371 6 17 4.6478780822071855 6 18 5.578403222518247 6 19 4.746794252685476 6 20 3.6764715200564595 6 21 3.9638415505093434 6 22 3.839223073181401 6 23 3.0249252729892646 6 24 4.0678430424383905 6 25 2.9659352946099986 6 26 2.9496080711638584 6 27 2.5766086140217794 6 28 2.7217068868219485 6 29 2.6314729698199386 6 30 2.541652843484542 6 31 2.1258261552951936 6 32 3.2071091432517136 6 33 2.6533679790526534 6 34 1.6009214252380772 6 35 1.6436766899327395 6 36 2.7720572246800756 6 37 1.0502503533865593 6 38 1.284162023348614 6 39 2.7751616852335133 6 40 5.653971307809506 6 41 1.5264588811361364 6 42 4.524825944229999 6 43 4.074568960046484 6 44 4.4640569296678265 6 45 3.9503867016883127 6 46 4.619423820566421 6 47 5.352866743167532 6 48 5.160390728313924 6 49 6.2809224924011 6 50 3.7326070069505306 6 51 3.549982076919303 6 52 4.904287259574485 6 53 5.231418194955641 6 54 5.822584301368806 6 55 4.659828390971555 6 56 6.12663536114869 7 1 0.47191940292763235 7 2 0.2813893151078375 7 3 0.38327526890216035 7 4 0.4736216118236527 7 5 1.1009639243277007 7 6 0.007692330016695337 7 7 -0.045682151770044754 7 8 0.2693661312151292 7 9 0.4536448991397677 7 10 -0.061915883290609486 7 11 2.781262961084032 7 12 4.919847372689119 7 13 4.861746914665424 7 14 4.878596124276861 7 15 4.183336452109728 7 16 3.6436131181275613 7 17 4.531450649990283 7 18 4.711577066218403 7 19 2.1503030246152974 7 20 3.5935369108060584 7 21 1.7692641980372557 7 22 1.3184894208688007 7 23 0.9810656238664563 7 24 1.4091508574304115 7 25 1.2641809086450806 7 26 1.1697037649437618 7 27 -0.026262199375345574 7 28 0.17465744503566327 7 29 -0.9913340477239245 7 30 0.1676496536918819 7 31 -1.1915582147207573 7 32 1.1369337046552204 7 33 -0.11537205284527374 7 34 -0.8926989894349688 7 35 -0.6816821617419038 7 36 -1.7119938120025395 7 37 0.7217017090484461 7 38 -1.5372095874966831 7 39 0.14198784153335708 7 40 4.182679441780799 7 41 -0.8427596196613116 7 42 1.9426887831454707 7 43 4.156706121131009 7 44 4.335667774962879 7 45 2.937627531034405 7 46 3.181624777504222 7 47 3.8456897096747085 7 48 3.669960106304355 7 49 4.411703991603652 7 50 3.1751907390079452 7 51 4.14806820132122 7 52 3.8380990618073376 7 53 3.7250386096286903 7 54 3.2787246522460016 7 55 2.426176078371874 7 56 2.841946365933378 8 1 0.11838404939227587 8 2 0.22583375955228036 8 3 0.5120631476900428 8 4 0.6125105007125455 8 5 0.045408368772143604 8 6 0.5480963704207298 8 7 0.4113885553006611 8 8 0.41078027262927463 8 9 0.21374590924077808 8 10 0.2966699752952522 8 11 1.286313466134537 8 12 4.328938281780026 8 13 2.988009540928047 8 14 4.277586023266764 8 15 3.428285947059223 8 16 5.047653522167963 8 17 3.071854690394325 8 18 4.666122520763857 8 19 3.2891919135041903 8 20 3.1238399411090896 8 21 3.203607632380688 8 22 3.29828740066678 8 23 1.8977322905331278 8 24 2.9041003523799063 8 25 1.7919586864228592 8 26 1.5636431588831528 8 27 0.6000004268872772 8 28 1.4600109803891996 8 29 0.7586659522760755 8 30 -0.20103721499499017 8 31 -0.4238814470439891 8 32 0.9702670379885561 8 33 0.0992744118011899 8 34 -0.018961615697598688 8 35 0.5910451109853696 8 36 1.1390162890075572 8 37 -0.38688414953741557 8 38 1.149659099372002 8 39 0.8818868314323467 8 40 4.114497623598979 8 41 -1.153365680267374 8 42 3.6169312073878963 8 43 3.4546859191108084 8 44 3.9114253507204566 8 45 2.9401527835596575 8 46 1.797786393665838 8 47 1.5224573864423832 8 48 0.9250106113548604 8 49 5.295542375442036 8 50 2.561554375371582 8 51 0.3803914336444514 8 52 2.0300182537265314 8 53 1.7679679025579809 8 54 3.917613541134891 8 55 3.140822543018338 8 56 2.2889160629030734 9 1 -0.2553533243450943 9 2 -0.5367925030739826 9 3 -0.4046035189766286 9 4 -0.5263783881763473 9 5 -0.444490621126846 9 6 -0.022610700286335828 9 7 -0.5002276063155051 9 8 -0.3973005354515351 9 9 -0.24332479782992777 9 10 -0.4558552772300004 9 11 2.053990233811298 9 12 4.162271615113362 9 13 4.164777217695729 9 14 4.8179900636708055 9 15 4.607578876352157 9 16 4.552704027218468 9 17 4.25872337726301 9 18 4.469152823794161 9 19 3.3624242367365085 9 20 3.6541429714121207 9 21 2.6177490465221 9 22 1.8639439663233475 9 23 2.678035320836159 9 24 2.9849084331879894 9 25 1.3853930298572052 9 26 1.1394007346407307 9 27 1.9434347703216233 9 28 0.4776877480659678 9 29 2.2510901947003177 9 30 0.9555284415706709 9 31 1.9296539064913603 9 32 1.0763276440491651 9 33 1.854324916851695 9 34 1.1679070711710864 9 35 2.2274087473490027 9 36 2.5001274001186715 9 37 2.4489744363211727 9 38 1.068851018563926 9 39 2.960169659715177 9 40 6.031164290265643 9 41 2.763300986399294 9 42 4.276022116478803 9 43 6.914281878706767 9 44 7.881122320417425 9 45 5.331566924973796 9 46 5.878594474473918 9 47 6.088113952098951 9 48 5.760869197213449 9 49 9.07837065827032 9 50 6.266099829917035 9 51 7.360189413442431 9 52 5.959311183019462 9 53 6.967462852052929 9 54 8.581754955276303 9 55 7.7898124420082375 9 56 5.205582729569741 10 1 1.1247332557414822 10 2 1.4242464579649834 10 3 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1.360275880216136 119 36 2.359101759093029 119 37 3.4186714060181416 119 38 3.3590608087737124 119 39 3.4694936690391813 119 40 7.941420700522059 119 41 4.878685601783911 119 42 -0.05614571568902704 119 43 -9.84445938003449 119 44 -7.6270361877410835 119 45 -5.90386431045744 119 46 -14.39879480291536 119 47 -10.545918681933685 119 48 -12.987382551038303 119 49 -12.636081556181896 119 50 -9.226907163089958 119 51 -7.5512324979794805 119 52 -7.897332173623898 119 53 -9.426476541886462 119 54 -9.08024970672836 119 55 -7.805758653562858 119 56 -5.179032655045642 120 1 -0.8657429347347048 120 2 -0.7186106848921625 120 3 -0.5950797094528184 120 4 -0.1930450548430116 120 5 0.005725829089605838 120 6 -0.793173470849105 120 7 -0.6560717621596552 120 8 -1.0812832194342192 120 9 -1.3299048844100128 120 10 -0.758885580260305 120 11 -6.015273835452767 120 12 -12.218680765839022 120 13 -11.194530141611633 120 14 -15.333525087844347 120 15 -13.115364846591568 120 16 -18.507902033387595 120 17 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-9.093304441108646 120 56 -3.4610839370969266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 63.0 5 2.0 6 14.0 7 23.0 8 53.0 9 79.0 10 32.0 11 37.0 12 35.0 13 34.0 14 57.0 15 51.0 16 71.0 17 91.0 18 131.0 19 160.0 20 193.0 21 272.0 22 294.0 23 419.0 24 454.0 25 499.0 26 566.0 27 684.0 28 779.0 29 976.0 30 1131.0 31 1305.0 32 1557.0 33 1756.0 34 2022.0 35 2244.0 36 2847.0 37 3610.0 38 3604.0 39 2402.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.29246935201401 0.24168126094570927 0.42732049036777586 0.03852889667250438 8 99.09632224168125 0.34325744308231176 0.4623467600700526 0.09807355516637478 9 97.14535901926445 1.2679509632224168 1.1243432574430823 0.4623467600700526 10 54.08056042031524 28.87215411558669 9.422066549912435 7.625218914185639 11 36.8476357267951 30.549912434325744 18.47985989492119 14.122591943957968 12 42.458844133099824 13.950963222416812 21.320490367775832 22.26970227670753 13 25.77254572209376 15.405367528554411 27.77660990820545 31.04547684114638 14 28.48436022277488 18.24932572069074 27.801324039370908 25.464990017163476 15 27.334012148449844 16.25294055686247 26.024367121940944 30.388680172746746 16 29.85456456982653 22.001051340459085 26.49377956895041 21.650604520763974 17 35.54816112084063 18.098073555166376 23.29246935201401 23.061295971978986 18 27.524606816350833 19.139024133945146 29.622753861781497 23.713615187922517 19 33.11592678308436 21.638964864296234 21.93351567431096 23.311592678308436 20 26.624833485241535 22.007992708406366 23.918530463436866 27.448643342915236 21 28.942206654991242 20.164623467600702 29.523642732049037 21.36952714535902 22 28.26970227670753 19.404553415061297 25.98949211908932 26.336252189141856 23 30.15411558669002 19.295971978984237 30.486865148861646 20.063047285464098 24 33.89492119089317 20.907180385288967 25.34851138353765 19.849387040280213 25 29.985989492119092 19.698774080560423 29.040280210157622 21.27495621716287 26 31.635548393876768 19.29099379969874 26.65428941745192 22.419168388972572 27 26.291425565491842 17.264597580220936 32.6775381378222 23.766438716465018 28 30.920060331825038 18.622189484022588 28.075344628012207 22.382405556140167 29 32.00546562959848 19.55714385817392 27.927265083035525 20.510125429192065 30 30.876221840731528 19.96286304873349 31.068913569001154 18.092001541533826 31 27.795101783399325 22.084019480746996 32.234329560982445 17.886549174871238 32 28.354022021179603 19.773476400869626 32.88449400378708 18.98800757416369 33 27.511667894866125 20.500403551251008 34.02112503070499 17.96680352317788 34 23.357510774729317 21.514418865412242 38.487683520796104 16.640386839062334 35 21.716888577435178 24.01541695865452 38.87175893482831 15.395935529081989 36 21.262132520410663 26.6652650758611 35.94029223168296 16.132310172045273 37 21.803060078607526 25.44567658618754 35.18037619314992 17.570887142055025 38 24.085130294303575 24.773477558474397 35.14083023108801 16.000561916134018 39 24.685810573615107 23.155234150108825 34.258934213297756 17.900021062978304 40 18.668642184358134 18.139485659858188 31.562422831340175 31.629449324443502 41 15.957897711217859 17.229443345577845 30.14269567674484 36.669963266459455 42 19.290924393323046 24.358255014728574 36.10955253191191 20.24126806003647 43 18.10235888795282 19.422212861555742 36.54872226902555 25.92670598146588 44 18.600802760368985 19.97042461798465 34.409548623336384 27.019223998309975 45 21.343818015867445 22.579512743101873 36.43193147511058 19.6447377659201 46 20.765775250930023 23.41896539622377 35.46711588404576 20.34814346880045 47 19.61533071748879 23.241311659192824 37.535033632286996 19.60832399103139 48 19.602133183636237 24.689495474001824 37.969265314714754 17.739106027647182 49 17.860160540768906 19.363469933812137 33.83326292071539 28.943106604703562 50 20.47191641539864 23.497650936119488 36.17207769441133 19.858354954070542 51 19.328321506358463 25.173891660226232 37.39197639288977 18.105810440525538 52 17.37696867655828 25.22010593146024 38.173909993335435 19.22901539864604 53 18.354097210036848 24.88857694332339 38.329531496753816 18.427794349885946 54 16.7995224216034 23.197668293710713 36.56635179267479 23.436457492011098 55 19.109488025844513 25.001755741273968 37.42538099585645 18.463375237025073 56 18.838392700473765 25.120196525706262 35.704509563081245 20.336901210738727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 10.0 11 19.0 12 38.0 13 65.5 14 74.0 15 101.5 16 129.0 17 158.5 18 188.0 19 201.5 20 215.0 21 234.5 22 299.0 23 344.0 24 468.0 25 592.0 26 698.5 27 805.0 28 885.0 29 1010.5 30 1056.0 31 1066.0 32 1076.0 33 1003.5 34 931.0 35 840.5 36 750.0 37 672.0 38 545.5 39 497.0 40 488.0 41 479.0 42 483.0 43 487.0 44 506.0 45 525.0 46 630.0 47 728.5 48 722.0 49 755.5 50 789.0 51 843.0 52 897.0 53 1008.5 54 1164.0 55 1208.0 56 1274.5 57 1341.0 58 1524.0 59 1707.0 60 1869.0 61 2031.0 62 1995.0 63 1836.0 64 1713.0 65 1466.0 66 1219.0 67 1098.0 68 977.0 69 861.5 70 746.0 71 614.5 72 428.5 73 374.0 74 312.0 75 250.0 76 191.0 77 132.0 78 94.5 79 42.5 80 28.0 81 22.5 82 17.0 83 10.0 84 3.0 85 3.0 86 3.0 87 1.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.028021015761821366 14 0.0035026269702276708 15 0.24168126094570927 16 0.05253940455341507 17 0.0 18 0.0035026269702276708 19 0.11208406304728546 20 0.0840630472854641 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.010507880910683012 27 0.12259194395796848 28 0.1436077057793345 29 0.028021015761821366 30 0.024518388791593695 31 0.03152364273204904 32 0.11208406304728546 33 0.18563922942206657 34 0.03852889667250438 35 0.03502626970227671 36 0.03852889667250438 37 0.18914185639229422 38 0.266199649737303 39 0.22416812609457093 40 0.7110332749562172 41 0.8336252189141855 42 0.1190893169877408 43 0.2171628721541156 44 0.5183887915936953 45 0.22416812609457093 46 0.19614711033274956 47 0.021015761821366025 48 0.1681260945709282 49 0.51138353765324 50 0.09807355516637478 51 0.29422066549912435 52 0.1436077057793345 53 0.1926444833625219 54 0.25569176882662 55 0.2521891418563923 56 0.1926444833625219 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 28550.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.82486865148861 #Duplication Level Percentage of deduplicated Percentage of total 1 86.93460551780008 67.65674255691769 2 7.7276205049732205 12.028021015761821 3 2.569872631531572 6.0 4 1.1071605382780503 3.446584938704028 5 0.5670822269229038 2.2066549912434326 6 0.3285476394077141 1.5341506129597198 7 0.18902740897430126 1.0297723292469352 8 0.16652414600117016 1.0367775831873907 9 0.09001305189252443 0.6304728546409807 >10 0.2970430712453306 3.1313485113835373 >50 0.018002610378504882 0.903677758318739 >100 0.004500652594626221 0.3957968476357268 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 113 0.3957968476357268 No Hit CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 81 0.28371278458844135 No Hit CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 64 0.22416812609457093 No Hit CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 58 0.2031523642732049 No Hit CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 55 0.1926444833625219 No Hit CTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 32 0.11208406304728546 No Hit CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 29 0.10157618213660245 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0070052539404553416 0.0 0.0 0.0 0.0 11 0.0070052539404553416 0.0 0.0 0.0 0.0 12 0.0070052539404553416 0.0 0.0 0.0 0.0 13 0.0070052539404553416 0.0 0.0 0.0 0.0 14 0.010507880910683012 0.0 0.0 0.0 0.0 15 0.010507880910683012 0.0 0.0 0.0 0.0 16 0.021015761821366025 0.0 0.0 0.0 0.0 17 0.021015761821366025 0.0 0.0 0.0 0.0 18 0.024518388791593695 0.0 0.0 0.0 0.0 19 0.028021015761821366 0.0 0.0 0.0 0.0 20 0.028021015761821366 0.0 0.0 0.0 0.0 21 0.03852889667250438 0.0 0.0 0.0 0.0 22 0.04203152364273205 0.0 0.0 0.0 0.0 23 0.04903677758318739 0.0 0.0 0.0 0.0 24 0.05604203152364273 0.0 0.0 0.0 0.0 25 0.0595446584938704 0.0 0.0 0.0 0.0 26 0.06304728546409807 0.0 0.0 0.0 0.0 27 0.07355516637478109 0.0 0.0 0.0 0.0 28 0.08056042031523643 0.0 0.0 0.0 0.0 29 0.0840630472854641 0.0 0.0 0.0 0.0 30 0.09106830122591944 0.0 0.0 0.0 0.0 31 0.09807355516637478 0.0 0.0 0.0 0.0 32 0.10157618213660245 0.0 0.0 0.0 0.0 33 0.10157618213660245 0.0 0.0 0.0 0.0 34 0.10507880910683012 0.0 0.0 0.0 0.0 35 0.10507880910683012 0.0 0.0 0.0 0.0 36 0.10507880910683012 0.0 0.0 0.0 0.0 37 0.10507880910683012 0.0 0.0 0.0 0.0 38 0.10507880910683012 0.0 0.0 0.0 0.0 39 0.1085814360770578 0.0 0.0 0.0 0.0 40 0.1085814360770578 0.0 0.0 0.0 0.0 41 0.1085814360770578 0.0 0.0 0.0 0.0 42 0.11558669001751314 0.0 0.0 0.0 0.0 43 0.11558669001751314 0.0 0.0 0.0 0.0 44 0.11558669001751314 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTGACG 15 0.008243059 50.15873 13 GAGTGGT 15 0.008300821 50.070423 11 GTACCTG 15 0.008300821 50.070423 9 CTGACGA 15 0.008300821 50.070423 14 GAGCAGT 15 0.008300821 50.070423 9 GGCCCGG 15 0.008300821 50.070423 9 TACCTGG 15 0.008300821 50.070423 10 TCATCTC 15 0.008300821 50.070423 15 CGACTGT 15 0.008300821 50.070423 46 GTACGCC 15 0.008300821 50.070423 9 AAGCATG 20 4.0863338E-4 50.07042 10 TAAGCTC 15 0.008358885 49.982426 49 GGATGGC 15 0.008417249 49.894737 8 CCAGTAG 15 0.008417249 49.894737 26 GGGTACC 20 4.1580698E-4 49.894737 7 GGACAGT 15 0.008417249 49.894737 8 GCCAGTA 15 0.008417249 49.894737 25 TGCCAGT 15 0.008417249 49.894737 24 CGGTTTC 15 0.008417249 49.894737 19 TCTTCCA 15 0.008417249 49.894737 50 >>END_MODULE