FastQCFastQC Report
Fri 17 Jun 2016
SRR1526238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44123
Sequences flagged as poor quality0
Sequence length56
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5931.343970264941187No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1590.3603562767717517No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1550.35129071006051266No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1230.27876617637060036No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.25156947623688325No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC450.10198762550143917No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA251.9526668E-550.43054241
TGCTTGT1100.050.4305437
TGCTTGC150.00809785650.4305437
ATATGCT1550.050.31500634
CTGAGTC402.5429472E-950.08551849
AACGATT451.2732926E-1050.08551847
ACGATTC204.1049195E-450.08551848
ACGATTA252.0338284E-550.08551848
CGGCCAA204.1049195E-450.08551845
GCCAAGG204.1049195E-450.08551847
CGATTAA252.0338284E-550.08551849
CACGCCG150.00835860250.02847743
TCGCGAC150.00835860250.02847713
TTGATAC150.00835860250.02847733
CAACGAT451.2914825E-1050.02847746
GTTCTGA507.2759576E-1250.02847346
CTGGTTG1900.050.02847313
GCTCCTG204.1281173E-450.02847333
GGCTCTC204.151419E-449.971569
CGCACTC204.151419E-449.9715615