FastQCFastQC Report
Fri 17 Jun 2016
SRR1526237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18296
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC460.2514210756449497No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC410.2240926978574552No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC380.20769567118495846No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC350.19129864451246176No Hit
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA220.12024486226497595No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT220.12024486226497595No Hit
ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG210.11477918670747705No Hit
ACACCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.10931351114997813No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG200.10931351114997813No Hit
ACACCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC190.10384783559247923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGGT150.00793999350.5598938
ACTGTTC150.00811702750.2797845
CCAAAGT150.00811702750.2797846
GTTCTTC150.00811702750.2797848
GAGCCTC150.00811702750.2797846
TTCTTCC150.00811702750.2797849
AAGTGCA150.00811702750.2797849
GCCAAAG150.00811702750.2797845
AAAGTGC150.00811702750.2797848
CTGTTCT150.00811702750.2797846
AGCTGCA150.00811702750.2797835
CGCCCGG150.00820663750.1408915
GAGCTGC150.00820663750.1408934
AGTGCAT150.00820663750.1408950
TCGTCTC150.00820663750.1408915
ATCGTCT150.00820663750.1408914
TCGGCTC150.008296980550.00275817
ACTAGCA150.008296980550.00275819
GCCCGGG150.008296980550.00275816
TTCGGCT150.008296980550.00275816
Produced by FastQC (version 0.11.5)