##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526237_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18296 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.75776125929165 39.0 38.0 39.0 35.0 40.0 2 37.18676213379974 39.0 38.0 39.0 34.0 40.0 3 37.1442391779624 39.0 38.0 39.0 34.0 40.0 4 36.77443156974202 39.0 38.0 39.0 32.0 40.0 5 36.97032138172278 39.0 38.0 39.0 33.0 40.0 6 36.91555531263664 39.0 38.0 39.0 33.0 40.0 7 36.42107564494972 39.0 36.0 39.0 32.0 40.0 8 36.67244206383909 39.0 37.0 39.0 32.0 40.0 9 36.78776781810232 39.0 38.0 39.0 33.0 40.0 10 36.16916265850459 39.0 36.0 39.0 31.0 40.0 11 35.62423480542195 38.0 35.0 39.0 30.0 40.0 12 33.42561215566244 38.0 32.0 39.0 18.0 40.0 13 33.23245518146043 37.0 32.0 39.0 18.0 40.0 14 33.46655006558811 38.0 32.0 39.0 19.0 40.0 15 33.4763882815916 38.0 33.0 39.0 19.0 40.0 16 32.73677306515085 37.0 30.0 39.0 18.0 40.0 17 32.78279405334499 36.0 30.0 39.0 19.0 40.0 18 32.26809138609532 36.0 29.0 39.0 17.0 40.0 19 33.1193156974202 36.0 31.0 39.0 22.0 40.0 20 32.85570616528203 36.0 30.0 39.0 21.0 40.0 21 33.692501093135114 38.0 33.0 39.0 23.0 40.0 22 33.431241801486664 37.0 32.0 39.0 22.0 40.0 23 33.68484914735461 38.0 33.0 39.0 23.0 40.0 24 33.40752076956712 37.0 32.0 39.0 22.0 40.0 25 33.52694578049847 37.0 32.0 39.0 23.0 40.0 26 34.060887625710535 38.0 33.0 39.0 25.0 40.0 27 33.879973764757324 38.0 33.0 39.0 24.0 40.0 28 33.810887625710535 38.0 33.0 39.0 23.0 40.0 29 33.67648666375164 37.0 33.0 39.0 23.0 40.0 30 33.441517271534764 37.0 33.0 39.0 23.0 40.0 31 33.54301486663751 37.0 33.0 39.0 23.0 40.0 32 33.10696327066025 37.0 33.0 39.0 20.0 40.0 33 33.2506558810669 37.0 33.0 39.0 23.0 40.0 34 32.99273065150852 36.0 33.0 39.0 20.0 40.0 35 32.86614560559685 36.0 33.0 39.0 18.0 40.0 36 32.03181023174464 35.0 31.0 38.0 16.0 39.0 37 31.702721906427634 35.0 31.0 38.0 13.0 39.0 38 31.422606034105815 35.0 30.0 38.0 10.0 39.0 39 31.14615216440752 35.0 30.0 38.0 4.0 39.0 40 28.122321818976825 34.0 20.0 38.0 4.0 39.0 41 25.050557498906866 30.0 11.0 36.0 4.0 39.0 42 28.40041539134237 33.0 25.0 37.0 4.0 39.0 43 27.09280717096633 32.0 21.0 36.0 4.0 39.0 44 27.062254044599914 32.0 21.0 36.0 4.0 39.0 45 27.995135548753826 33.0 25.0 36.0 4.0 39.0 46 27.606416703104504 33.0 23.0 38.0 4.0 39.0 47 27.206711849584607 33.0 21.0 37.0 4.0 39.0 48 27.19687363358111 33.0 21.0 37.0 4.0 39.0 49 24.85013117621338 31.0 9.0 36.0 4.0 39.0 50 26.77290118058592 33.0 19.0 37.0 4.0 39.0 51 26.99573677306515 33.0 17.0 38.0 4.0 39.0 52 26.57165500655881 33.0 11.0 38.0 4.0 39.0 53 26.210811106252734 33.0 4.0 38.0 4.0 39.0 54 25.381394840402272 32.0 4.0 37.0 4.0 39.0 55 25.563839090511586 32.0 4.0 37.0 4.0 39.0 56 24.650797988631396 32.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.29505099704635285 1 2 1.4193440540993407 1 3 1.1753788076864709 1 4 1.7573465402684292 1 5 1.8177443474496613 1 6 1.2921783320844256 1 7 1.5895102059427373 1 8 1.061628772596201 1 9 1.6742135008561618 1 10 1.1380911866067578 1 11 -2.1982862568605768 1 12 -11.42926024554103 1 13 -7.754257188666955 1 14 -4.996404343242546 1 15 -8.665991903212195 1 16 -22.179435801746425 1 17 -6.109010725186497 1 18 -14.361649870455292 1 19 -4.709299147567755 1 20 -4.855828710839802 1 21 -4.708974852420447 1 22 -1.9424707486763921 1 23 -2.693187747688331 1 24 -4.037182531852881 1 25 -1.6173506691434163 1 26 2.0090583788631307 1 27 2.955727595571794 1 28 3.626019189019374 1 29 3.4756137448837663 1 30 3.674572727549325 1 31 4.365913826438508 1 32 4.831364186809843 1 33 4.024026066829883 1 34 2.39621963493493 1 35 3.347601454326192 1 36 3.8779905763852867 1 37 5.9542840752467185 1 38 5.911992931157926 1 39 4.369170189827276 1 40 6.064233250095011 1 41 -11.806436537910015 1 42 -9.585269556349878 1 43 -16.025580146843982 1 44 -19.216180958602077 1 45 -11.042384540558714 1 46 -16.32509211477526 1 47 -20.185407285614893 1 48 -14.464176525909618 1 49 -17.683059651753553 1 50 -12.399918783436776 1 51 -10.583351683261439 1 52 -10.23920461754304 1 53 -10.388488517983067 1 54 -9.59124184608853 1 55 -9.882944462575912 1 56 -5.87147360673314 2 1 1.4140986160939732 2 2 2.038391673146961 2 3 1.7289502362579015 2 4 1.852584635506524 2 5 2.5617919664972817 2 6 2.2147973797034766 2 7 1.904986396418927 2 8 2.3235335345009602 2 9 2.5551658818085414 2 10 2.0785673770829476 2 11 1.7659994574251385 2 12 -4.476879293160078 2 13 -2.3018762362860024 2 14 1.5928813710431697 2 15 2.756627144406849 2 16 2.011040388729768 2 17 3.039798798623025 2 18 -3.665221299026719 2 19 1.9752246619560552 2 20 3.322742717731625 2 21 2.796977528531933 2 22 2.587291156085513 2 23 2.1401455856450013 2 24 0.8854365157661661 2 25 2.965982664189916 2 26 1.5626298074345613 2 27 2.628346643190838 2 28 1.5545906175907973 2 29 1.6958518401218612 2 30 3.365048918025515 2 31 2.3540090645337486 2 32 3.265887996333653 2 33 1.5537879715917882 2 34 2.45574344445874 2 35 2.484506216230951 2 36 2.5565620049567173 2 37 4.007855503818142 2 38 3.090564359729356 2 39 3.0655987612558455 2 40 5.302328488190248 2 41 -8.723103204576681 2 42 -6.841221937302258 2 43 -14.983913480177316 2 44 -15.132847625268745 2 45 -9.560241683415857 2 46 -9.634615924299066 2 47 -16.27469299990061 2 48 -7.851081287814381 2 49 -9.962821556515456 2 50 -8.072537831055826 2 51 -2.6785897784995356 2 52 -2.1499189032573263 2 53 -2.1206313751259245 2 54 -1.8590989889456715 2 55 -1.591277795909246 2 56 1.432097821838287 3 1 1.1540986160939752 3 2 2.065058339813625 3 3 2.043950236257899 3 4 2.225917968839859 3 5 2.863458633163944 3 6 2.806464046370138 3 7 2.086653063085592 3 8 2.2768668678342934 3 9 2.895165881808545 3 10 2.341900710416283 3 11 1.6626661240917997 3 12 4.776454040173256 3 13 2.8914570970473292 3 14 4.03788137104317 3 15 4.1549604777401825 3 16 5.397707055396431 3 17 5.028132131956362 3 18 5.969778700973279 3 19 4.820224661956054 3 20 4.9327427177316245 3 21 4.111977528531931 3 22 3.8422911560855155 3 23 4.560145585645003 3 24 4.410436515766165 3 25 2.515982664189913 3 26 3.3842964741012267 3 27 1.0566799765241726 3 28 2.2679239509241356 3 29 2.1308518401218635 3 30 1.9850489180255124 3 31 0.8440090645337506 3 32 1.9342213296669897 3 33 1.6887879715917862 3 34 -1.0342565555412584 3 35 2.1228395495642864 3 36 2.3865620049567156 3 37 2.036188837151478 3 38 2.68723102639602 3 39 2.3939320945891787 3 40 6.008995154856915 3 41 6.526896795423319 3 42 2.9671113960310755 3 43 -2.6655801468439826 3 44 -0.8828476252687452 3 45 2.508091649917475 3 46 1.6387174090342675 3 47 -5.59302633323394 3 48 2.2939187121856186 3 49 0.7188451101512108 3 50 1.5391288356108426 3 51 2.948076888167133 3 52 0.9950810967426733 3 53 1.154368624874074 3 54 1.2792343443876604 3 55 2.170388870757421 3 56 3.557097821838287 4 1 2.07409861609397 4 2 2.9850583398136266 4 3 2.243950236257902 4 4 3.3859179688398626 4 5 3.4234586331639463 4 6 3.246464046370143 4 7 2.6866530630855934 4 8 3.2768668678342934 4 9 3.1351658818085397 4 10 3.301900710416284 4 11 3.8626661240918025 4 12 5.616454040173252 4 13 6.571457097047329 4 14 5.917881371043173 4 15 5.874960477740181 4 16 6.957707055396433 4 17 7.028132131956362 4 18 7.009778700973278 4 19 5.780224661956055 4 20 6.892742717731625 4 21 6.191977528531936 4 22 6.322291156085512 4 23 5.960145585645002 4 24 5.690436515766166 4 25 5.875982664189912 4 26 3.664296474101228 4 27 3.856679976524177 4 28 4.347923950924134 4 29 3.050851840121858 4 30 4.825048918025516 4 31 3.764009064533745 4 32 4.454221329666986 4 33 4.328787971591787 4 34 2.8457434444587406 4 35 3.762839549564287 4 36 4.226562004956719 4 37 4.356188837151478 4 38 3.447231026396018 4 39 4.953932094589181 4 40 8.368995154856915 4 41 8.886896795423318 4 42 6.007111396031075 4 43 7.094419853156015 4 44 5.997152374731254 4 45 6.1880916499174745 4 46 5.918717409034265 4 47 5.966973666766059 4 48 6.493918712185618 4 49 8.39884511015121 4 50 6.459128835610841 4 51 4.588076888167134 4 52 7.235081096742675 4 53 7.354368624874077 4 54 7.719234344387662 4 55 4.9703888707574215 4 56 2.2370978218382866 5 1 1.7057652827606375 5 2 2.7467250064802897 5 3 1.8122835695912372 5 4 2.852584635506524 5 5 3.0617919664972817 5 6 3.131464046370141 5 7 1.9466530630855914 5 8 3.031866867834296 5 9 2.513499215141877 5 10 2.370234043749619 5 11 3.682666124091803 5 12 5.68978737350659 5 13 5.073123763713994 5 14 4.884548037709834 5 15 5.6732938110735205 5 16 5.886040388729768 5 17 5.289798798623025 5 18 5.1264453676399455 5 19 4.225224661956055 5 20 4.489409384398289 5 21 4.296977528531933 5 22 4.462291156085513 5 23 3.3068122523116656 5 24 5.17710318243283 5 25 4.6743159975232516 5 26 3.0626298074345613 5 27 5.086679976524174 5 28 4.304590617590797 5 29 3.9875185067885255 5 30 3.7817155846921793 5 31 2.270675731200413 5 32 3.765887996333653 5 33 4.5954546382584525 5 34 4.497410111125404 5 35 3.109506216230951 5 36 4.806562004956717 5 37 5.174522170484813 5 38 6.0072310263960205 5 39 3.023932094589181 5 40 8.51066182152358 5 41 10.943563462089983 5 42 8.32544472936441 5 43 9.516086519822682 5 44 10.117152374731255 5 45 8.898091649917475 5 46 9.032050742367602 5 47 8.600307000099395 5 48 6.815585378852283 5 49 10.745511776817875 5 50 8.594128835610842 5 51 8.363076888167132 5 52 7.016747763409342 5 53 9.004368624874076 5 54 7.057567677720993 5 55 6.408722204090754 5 56 7.682097821838287 6 1 1.5390986160939732 6 2 2.5800583398136254 6 3 2.2289502362579015 6 4 2.727584635506524 6 5 2.9367919664972817 6 6 3.0897973797034766 6 7 2.1133197297522557 6 8 1.781866867834296 6 9 2.5551658818085414 6 10 1.745234043749619 6 11 3.0159994574251385 6 12 5.231454040173254 6 13 4.573123763713994 6 14 4.96788137104317 6 15 4.756627144406849 6 16 5.8443737220630965 6 17 6.4981321319563605 6 18 6.043112034306617 6 19 4.808557995289391 6 20 4.239409384398289 6 21 5.005310861865269 6 22 4.420624489418849 6 23 4.848478918978337 6 24 4.260436515766166 6 25 4.00764933085658 6 26 2.8542964741012256 6 27 4.545013309857509 6 28 3.637923950924133 6 29 4.320851840121861 6 30 4.906715584692179 6 31 4.312342397867084 6 32 4.0575546630003245 6 33 3.887121304925124 6 34 3.6640767777920757 6 35 2.4011728828976224 6 36 4.306562004956717 6 37 3.3411888371514777 6 38 3.465564359729356 6 39 5.982265427922517 6 40 8.51066182152358 6 41 4.026896795423319 6 42 7.742111396031074 6 43 8.724419853156018 6 44 7.4504857080645905 6 45 8.648091649917475 6 46 7.698717409034266 6 47 6.808640333432724 6 48 7.023918712185619 6 49 9.32884511015121 6 50 8.969128835610842 6 51 6.571410221500464 6 52 7.141747763409342 6 53 5.421035291540743 6 54 4.557567677720993 6 55 5.74205553742409 6 56 4.932097821838287 7 1 1.2217909237862798 7 2 2.474289109044392 7 3 1.6424117747194416 7 4 3.0320718149937065 7 5 2.9111509408562526 7 6 2.8526178925239876 7 7 2.9081915246240513 7 8 2.4260976370650624 7 9 2.324396651039308 7 10 3.1619007104162833 7 11 2.336512277937956 7 12 4.9333771170963345 7 13 5.146841712431943 7 14 5.67941983258163 7 15 3.9841912469709513 7 16 5.446937824627199 7 17 3.440439824264054 7 18 5.555932547127128 7 19 5.100224661956055 7 20 6.072742717731625 7 21 5.710439066993473 7 22 4.6930603868547465 7 23 3.6401455856450013 7 24 5.548898054227706 7 25 5.177521125728376 7 26 3.6812195510243058 7 27 3.990526130370327 7 28 2.926385489385673 7 29 5.070851840121861 7 30 4.6342796872562815 7 31 3.680932141456829 7 32 3.012682868128529 7 33 2.774941817745635 7 34 3.0134357521510466 7 35 1.1351472418719801 7 36 3.9604081588028706 7 37 4.177727298689938 7 38 3.0072310263960205 7 39 3.697009017666101 7 40 5.930533616395376 7 41 1.026896795423319 7 42 5.0594190883387675 7 43 6.474419853156018 7 44 5.232536990115872 7 45 3.4173224191482454 7 46 5.775640485957343 7 47 5.959281359073753 7 48 6.350841789108696 7 49 8.155768187074287 7 50 5.796051912533919 7 51 7.911153811244052 7 52 6.975081096742674 7 53 6.292830163335616 7 54 7.599234344387661 7 55 7.565773486142039 7 56 5.48017474491521 8 1 1.783663833485278 8 2 2.748536600683188 8 3 1.9952545840839875 8 4 3.2293962297094225 8 5 3.103458633163946 8 6 3.0934205681092735 8 7 3.359696541346459 8 8 2.5916494765299447 8 9 2.968209360069409 8 10 2.9662485365032367 8 11 3.921796558874412 8 12 6.595584474955864 8 13 5.1836310100908065 8 14 6.239620501477951 8 15 6.019308303827138 8 16 6.764663577135565 8 17 5.443784305869407 8 18 6.905430874886328 8 19 4.578485531521274 8 20 6.529264456862062 8 21 5.063281876358019 8 22 5.744899851737685 8 23 4.640145585645001 8 24 4.575653907070517 8 25 3.9768522294073065 8 26 3.1042964741012256 8 27 3.4236364982633063 8 28 4.127054385706742 8 29 4.136069231426212 8 30 5.125918483242906 8 31 4.1692264558381 8 32 4.49596046010177 8 33 5.189657536809179 8 34 4.162265183589177 8 35 4.8776221582599355 8 36 5.045692439739327 8 37 5.324884489325392 8 38 5.224622330743848 8 39 5.882627746763095 8 40 6.795082111378658 8 41 1.7877663606407097 8 42 5.975807048204988 8 43 8.53963724446037 8 44 8.073674113861692 8 45 7.756787302091389 8 46 7.633500017729915 8 47 7.02784323198345 8 48 6.947831755663877 8 49 10.687540762325124 8 50 7.219128835610842 8 51 7.16198993164539 8 52 7.475081096742674 8 53 9.276107755308857 8 54 6.773147387865922 8 55 4.537345392496551 8 56 4.774489126186111 9 1 1.4140986160939732 9 2 2.614149248904532 9 3 2.149404781712448 9 4 3.3677361506580397 9 5 3.103458633163946 9 6 2.6882822281883207 9 7 0.9921076085401381 9 8 1.975048686016116 9 9 1.646074972717635 9 10 2.02553707405265 9 11 2.5463024877281697 9 12 5.083726767445981 9 13 4.53145709704733 9 14 4.71788137104317 9 15 5.124051386831091 9 16 4.904979782669159 9 17 5.975404859229087 9 18 4.300687791882375 9 19 4.3729519346833285 9 20 4.572742717731625 9 21 4.0356138921683 9 22 3.8713820651764195 9 23 2.2765092220086345 9 24 3.4649819703116194 9 25 5.397800846008096 9 26 4.058841928646679 9 27 4.654861794705994 9 28 3.6606512236514064 9 29 3.934488203758228 9 30 3.4105034634800617 9 31 4.694918155442842 9 32 5.156039511485169 9 33 4.246969789773608 9 34 4.52392526264056 9 35 3.8519304586551932 9 36 6.079289277683991 9 37 6.307097928060571 9 38 5.6890492082142 9 39 6.546659367316455 9 40 8.378086063947823 9 41 1.3905331590596823 9 42 8.0943841233038 9 43 9.656238034974198 9 44 9.480788738367622 9 45 7.011728013553842 9 46 10.198717409034266 9 47 10.403337303129693 9 48 9.864827803094713 9 49 10.57884511015121 9 50 10.764583381065389 9 51 9.942622342712585 9 52 9.565990187651767 9 53 10.936186806692255 9 54 11.644688889842207 9 55 8.450388870757422 9 56 8.92073418547465 10 1 2.0504622524576064 10 2 2.477785612540899 10 3 2.4221320544397216 10 4 3.3677361506580397 10 5 3.1489131786184927 10 6 3.1882822281883207 10 7 2.8102894267219583 10 8 3.0659577769252024 10 9 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11 -2.6744767330510513 115 12 -16.35783167411246 115 13 -11.539971474381243 115 14 -11.067832914671115 115 15 -8.642182379402673 115 16 -8.608007230317853 115 17 -2.609010725186497 115 18 -2.718792727598146 115 19 -2.471203909472518 115 20 -2.2129715679826596 115 21 2.6005489571033635 115 22 3.2480054417997977 115 23 1.7115741570735707 115 24 1.153293658623312 115 25 2.430268378475631 115 26 1.2471536169583715 115 27 2.408108547952743 115 28 1.1736382366384177 115 29 0.9279946972647153 115 30 -1.063522510545912 115 31 -0.11027664975196672 115 32 -0.4543500989044382 115 33 0.7859308287346423 115 34 1.4914577301730247 115 35 -1.2000175932928556 115 36 3.0208477192424326 115 37 3.573331694294332 115 38 4.221516740681736 115 39 1.4167892374463236 115 40 3.6118522977140586 115 41 1.526896795423319 115 42 -1.8471743182546412 115 43 -10.45415157541541 115 44 -7.5971333395544605 115 45 -2.7090512072253823 115 46 -7.944139733822876 115 47 -7.661597761805368 115 48 -6.797509859242954 115 49 -13.992583461277361 115 50 -8.066585450103442 115 51 -6.011923111832868 115 52 -5.667776046114469 115 53 -6.2456313751259245 115 54 -7.186479941326624 115 55 -5.19246827209972 115 56 -3.514330749590286 116 1 1.364098616093976 116 2 2.1550583398136283 116 3 1.9539502362579029 116 4 2.6359179688398626 116 5 2.553458633163949 116 6 2.4564640463701437 116 7 2.796653063085593 116 8 2.6068668678342988 116 9 2.45516588180854 116 10 3.0119007104162847 116 11 -0.7173338759081958 116 12 -13.243545959826747 116 13 -7.818542902952672 116 14 -3.1321186289568317 116 15 -3.935039522259814 116 16 -2.322292944603568 116 17 -0.5518678680436402 116 18 -6.5902212990267195 116 19 -1.4997753380439462 116 20 -1.7272572822683756 116 21 5.3219775285319315 116 22 5.212291156085513 116 23 5.490145585645003 116 24 4.8604365157661675 116 25 5.165982664189919 116 26 5.404296474101223 116 27 4.7866799765241765 116 28 4.537923950924132 116 29 5.370851840121858 116 30 5.665048918025512 116 31 4.104009064533749 116 32 5.024221329666986 116 33 4.328787971591787 116 34 3.8057434444587415 116 35 5.742839549564284 116 36 5.956562004956716 116 37 6.066188837151479 116 38 5.307231026396018 116 39 6.623932094589183 116 40 9.918995154856919 116 41 6.426896795423318 116 42 1.6171113960310741 116 43 -7.575580146843983 116 44 -3.382847625268745 116 45 1.4980916499174768 116 46 -4.301282590965734 116 47 -3.133026333233939 116 48 -2.7760812878143817 116 49 -3.7211548898487905 116 50 -2.880871164389159 116 51 -1.8119231118328685 116 52 -1.2749189032573263 116 53 -3.2456313751259245 116 54 -3.6007656556123386 116 55 0.25038887075742267 116 56 0.8070978218382869 117 1 0.48552718752254265 117 2 0.4193440540993407 117 3 0.8182359505436168 117 4 1.400203683125575 117 5 1.3177443474496613 117 6 1.363606903512995 117 7 1.1609387773713067 117 8 -0.6288474178799888 117 9 1.5551658818085414 117 10 2.1619007104162833 117 11 -3.317333875908197 117 12 -11.214974531255319 117 13 -11.325685760095528 117 14 -17.067832914671115 117 15 -11.49932523654553 117 16 -15.822292944603568 117 17 -15.537582153757926 117 18 -18.43307844188386 117 19 -8.899775338043945 117 20 -10.21297156798266 117 21 -0.6851653286109247 117 22 0.5337197275140824 117 23 0.49728844278785544 117 24 -1.3467063413766915 117 25 -0.28401733581008415 117 26 1.9614393312440797 117 27 1.6223942622384584 117 28 2.4593525223527024 117 29 1.0708518401218612 117 30 1.0079060608826609 117 31 1.0325804931051792 117 32 -0.5972072417615841 117 33 -1.2140691712653542 117 34 -0.3656851269698329 117 35 -0.5571604504357133 117 36 1.5208477192424326 117 37 -0.3552397342770952 117 38 1.5786595978245934 117 39 1.559646380303466 117 40 6.111852297714062 117 41 2.526896795423319 117 42 -1.061460032540353 117 43 -6.88272300398684 117 44 -9.097133339554459 117 45 -0.4233369215110976 117 46 -1.5869968766800184 117 47 -2.875883476091083 117 48 -3.5117955735286657 117 49 -3.135440604134505 117 50 -2.3522997358177307 117 51 1.1309340310242746 117 52 0.26079538245695844 117 53 -2.1742028036973515 117 54 -4.472194227040912 117 55 -1.4067539863854357 117 56 -2.1571878924474284 118 1 0.29871400070936005 118 2 0.8589044936597787 118 3 1.2193348516425146 118 4 1.801302584224473 118 5 1.7188432485485592 118 6 0.31415635406244746 118 7 1.9081915246240513 118 8 1.3107130216804492 118 9 1.5167043433470013 118 10 0.9695930181085899 118 11 -1.740410798985117 118 12 -9.835853652134439 118 13 -12.160850595260364 118 14 -12.205195552033754 118 15 -9.746577983798275 118 16 -11.245369867680491 118 17 -9.79032940650518 118 18 -11.713298222103642 118 19 -3.822852261120868 118 20 -3.196488051499145 118 21 1.1335159900703928 118 22 1.962291156085513 118 23 1.947837893337308 118 24 0.5488980542277062 118 25 0.7159826641899159 118 26 4.027373397178145 118 27 1.7212953611395605 118 28 3.1571547201548995 118 29 3.955467224737248 118 30 2.7112027641793617 118 31 2.4501629106875953 118 32 -0.6411632857176279 118 33 1.6980187408225547 118 34 2.12882036753566 118 35 3.7505318572565933 118 36 2.3065620049567173 118 37 4.793111914074558 118 38 4.776461795626787 118 39 3.658547479204568 118 40 5.968995154856916 118 41 -4.473103204576681 118 42 -7.9405809116612325 118 43 -13.56404168530552 118 44 -13.882847625268745 118 45 -7.851908350082525 118 46 -12.108974898658042 118 47 -11.156103256310864 118 48 -14.76454282627592 118 49 -13.075001043694943 118 50 -11.47317885669685 118 51 -13.165769265679021 118 52 -11.832611210949635 118 53 -12.091785221279771 118 54 -15.246919501766186 118 55 -13.703457283088731 118 56 -11.365979101238636 119 1 0.7964515572704443 119 2 1.3521171633430384 119 3 0.36865611861084346 119 4 1.7741532629575048 119 5 1.691693927281591 119 6 1.7711699287230829 119 7 1.6819471807326494 119 8 1.392160985481354 119 9 1.4081070582791284 119 10 0.6619007104162833 119 11 -3.7585103464964327 119 12 -8.261193018650275 119 13 -10.115601726482083 119 14 -7.223295099545066 119 15 -4.049745404612757 119 16 -12.498763532838861 119 17 -14.428338456278933 119 18 -16.319633063732603 119 19 -7.84095180863218 119 20 -9.015492576386023 119 21 -1.857434236173951 119 22 -1.2141794321497805 119 23 -2.4775014731785276 119 24 -1.754269366586776 119 25 -2.636958512280671 119 26 0.10429647410122556 119 27 1.6307976235829997 119 28 0.56439453915943 119 29 0.2179106636512742 119 30 -0.9584804937391915 119 31 -4.454814464878016 119 32 -8.437543376215363 119 33 -3.6594473225258604 119 34 -3.79425655554126 119 35 -3.263042803376891 119 36 -1.9875556421021052 119 37 -0.46028175108381575 119 38 -3.404533679486331 119 39 -3.4319502583519963 119 40 0.7337010372098582 119 41 -3.826044381047268 119 42 -7.456418015733632 119 43 -9.731462499785158 119 44 -8.882847625268745 119 45 -9.79308482067076 119 46 -17.683635532142205 119 47 -15.291849862645705 119 48 -16.873140111343794 119 49 -15.097625478084083 119 50 -16.251459399683277 119 51 -14.717805464774044 119 52 -13.81903655031615 119 53 -13.245631375125924 119 54 -11.871353890906457 119 55 -14.020199364536696 119 56 -11.384078648749949 120 1 -0.41923471723936245 120 2 0.5383916731469611 120 3 0.02061690292456575 120 4 0.8525846355065241 120 5 0.7701252998306103 120 6 0.8397973797034766 120 7 1.1966530630855914 120 8 0.8235335345009602 120 9 0.7218325484752057 120 10 0.995234043749619 120 11 -3.984000542574865 120 12 -14.726879293160078 120 13 -11.801876236286002 120 14 -16.448785295623495 120 15 -16.36837285559315 120 16 -20.072292944603568 120 17 -16.00186786804364 120 18 -16.206887965693383 120 19 -10.066442004710613 120 20 -14.093923948935043 120 21 -5.994689138134735 120 22 -5.037708843914487 120 23 -5.359854414354999 120 24 -10.072896817567166 120 25 -4.534017335810084 120 26 0.6876298074345613 120 27 0.253346643190838 120 28 0.5545906175907973 120 29 0.6541851734551969 120 30 1.4483822513588507 120 31 -0.9793242687995836 120 32 -12.025778670333011 120 33 -0.654545361741544 120 34 -1.54425655554126 120 35 0.3595062162309546 120 36 1.389895338290053 120 37 0.7161888371514777 120 38 0.3405643597293526 120 39 1.190598761255849 120 40 4.135661821523584 120 41 -1.973103204576681 120 42 -7.466221937302258 120 43 -9.442246813510648 120 44 -6.632847625268745 120 45 -9.601908350082525 120 46 -12.384615924299068 120 47 -15.399692999900607 120 48 -11.892747954481047 120 49 -15.587821556515458 120 50 -12.447537831055824 120 51 -10.511923111832868 120 52 -10.191585569923992 120 53 -9.66229804179259 120 54 -11.567432322279005 120 55 -8.049611129242578 120 56 -6.109568844828381 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 287.0 5 73.0 6 17.0 7 24.0 8 63.0 9 97.0 10 60.0 11 57.0 12 50.0 13 60.0 14 68.0 15 65.0 16 79.0 17 111.0 18 131.0 19 160.0 20 204.0 21 211.0 22 287.0 23 304.0 24 374.0 25 379.0 26 379.0 27 450.0 28 525.0 29 573.0 30 682.0 31 848.0 32 931.0 33 1118.0 34 1313.0 35 1595.0 36 2022.0 37 2189.0 38 1746.0 39 764.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 98.26191517271535 0.7269348491473546 0.3989943156974202 0.6121556624398776 8 98.5024048972453 0.9674245736773066 0.3115435067774377 0.21862702229995626 9 97.41473546130301 1.4374726716222126 0.5520332313073896 0.5957586357673808 10 57.597289024923484 26.524923480542196 8.783340620900743 7.094446873633581 11 40.101661565369476 27.39943156974202 14.811980760822038 17.686926104066465 12 35.13336248360297 13.975732400524704 24.464363795365106 26.426541320507212 13 23.29433632188935 16.21473868357752 26.295648370872517 34.19527662366061 14 23.74836029733275 19.29383471797114 28.421512898994315 28.536292085701792 15 26.232741617357004 17.833662064431298 27.865439403900943 28.06815691431076 16 29.87361164304864 18.805055534278054 24.632051211905672 26.68928161076763 17 30.15959772627897 20.173808482728464 22.40380411018802 27.26278968080455 18 29.0118058592042 21.89549628334062 22.146917358985572 26.945780498469613 19 30.19476966845388 23.913994966626547 19.482437903490535 26.40879746142904 20 29.154359479269225 23.405535499398315 21.605951208839294 25.83415381249316 21 29.995627459554004 20.326847398338437 23.480542195015303 26.196982947092263 22 29.558373414954087 19.075207695671185 24.10909488412768 27.257324005247046 23 28.95168342807171 20.977262789680804 25.371665937909928 24.69938784433756 24 29.241364232619148 22.119588981198078 22.54591167468299 26.093135111499784 25 26.820069960647135 22.90664626147792 23.994315697420202 26.278968080454746 26 26.951246174027112 20.359641451683427 25.05465675557499 27.63445561871447 27 25.52388247524211 18.96919625759151 27.592055588991627 27.914865678174756 28 25.02462515048703 21.823355587173033 25.839991244390937 27.312028017948997 29 27.12512983108293 21.19936587765812 27.29459356037829 24.380910730880665 30 24.801836768162687 20.291914940141037 31.65691794675559 23.249330344940688 31 26.52414019355897 19.744108480507407 32.117666356826504 21.614084969107115 32 28.41356673960613 17.35229759299781 32.36323851203501 21.87089715536105 33 27.347385710375033 17.33917328223378 33.796879277306324 21.516561730084863 34 23.071034067917097 19.42363427571499 37.928583146497516 19.576748509870402 35 22.972603488817192 22.753868868595177 35.93262973697162 18.34089790561601 36 25.174939864421603 21.81828121583206 33.686857642685325 19.31992127706101 37 25.429854342350232 21.706275325813166 33.51221114883364 19.351659183002955 38 25.994520547945204 20.684931506849317 34.74520547945205 18.575342465753426 39 25.224534501642935 20.21905805038335 33.98138006571742 20.575027382256298 40 18.866989649856862 14.991191367540187 29.85025324818322 36.291565734419734 41 15.529217199558987 15.259095920617419 28.776185226019845 40.43550165380375 42 19.670516118439057 18.95900607520114 37.15724371955558 24.213234086804224 43 18.403462634231865 15.861275476660092 36.48367302213456 29.25158886697348 44 19.4010989010989 16.98901098901099 35.85164835164835 27.75824175824176 45 23.126643295354953 18.25153374233129 37.02344434706398 21.59837861524978 46 21.711030781027496 19.465439807207797 36.3347573666338 22.4887720451309 47 20.97982393788616 19.43244573240746 37.033189348788895 22.554540980917494 48 21.51108677799069 18.998083766767042 38.56556255132767 20.92526690391459 49 18.544974998626298 16.336062421012144 33.084235397549314 32.03472718281225 50 20.98954627551858 19.823764435444147 37.173663182091836 22.013026106945432 51 20.999835553362935 21.049169544482815 36.08507372690895 21.8659211752453 52 20.632661996497372 20.884413309982484 37.41790718038529 21.06501751313485 53 20.32199770001643 21.291276490882208 37.30901922129128 21.077706587810088 54 19.28093828784391 19.69198728488436 34.81859037597281 26.208484051298914 55 21.511914543960557 20.372500684743905 36.417419884963024 21.69816488633251 56 21.85104052573932 21.80722891566265 34.26067907995619 22.08105147864184 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 2.0 11 3.0 12 2.5 13 6.5 14 11.0 15 17.5 16 24.0 17 30.5 18 37.0 19 38.0 20 39.0 21 84.0 22 143.0 23 157.0 24 182.0 25 207.0 26 245.0 27 283.0 28 373.0 29 502.5 30 542.0 31 614.0 32 686.0 33 666.5 34 647.0 35 545.5 36 444.0 37 395.5 38 291.0 39 235.0 40 238.5 41 242.0 42 232.0 43 222.0 44 238.0 45 254.0 46 275.0 47 329.5 48 363.0 49 384.0 50 405.0 51 425.0 52 445.0 53 513.0 54 667.5 55 754.0 56 769.0 57 784.0 58 835.5 59 887.0 60 1035.5 61 1184.0 62 1309.0 63 1423.0 64 1412.0 65 1340.5 66 1269.0 67 1153.5 68 1038.0 69 907.0 70 776.0 71 637.5 72 480.5 73 462.0 74 366.0 75 270.0 76 255.0 77 240.0 78 183.5 79 91.5 80 56.0 81 38.0 82 20.0 83 14.0 84 8.0 85 4.0 86 0.0 87 0.5 88 1.0 89 1.0 90 1.0 91 1.0 92 1.5 93 2.0 94 1.5 95 1.0 96 2.5 97 2.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.021862702229995628 14 0.0 15 0.2404897245299519 16 0.10384783559247923 17 0.0 18 0.0 19 0.09838216003498032 20 0.0765194578049847 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.10384783559247923 28 0.12024486226497595 29 0.01639702667249672 30 0.01639702667249672 31 0.03825972890249235 32 0.08745080891998251 33 0.1694359422824661 34 0.04919108001749016 35 0.04919108001749016 36 0.021862702229995628 37 0.18583296895496282 38 0.2514210756449497 39 0.19676432006996064 40 0.7214691735898557 41 0.8526453869698294 42 0.13664188893747267 43 0.2404897245299519 44 0.524704853519895 45 0.21862702229995626 46 0.20769567118495846 47 0.03825972890249235 48 0.1694359422824661 49 0.530170529077394 50 0.13664188893747267 51 0.28968080454744205 52 0.13117621337997376 53 0.19129864451246176 54 0.27328377787494534 55 0.2240926978574552 56 0.19676432006996064 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 18296.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.09925666812418 #Duplication Level Percentage of deduplicated Percentage of total 1 87.32034273079049 69.0697420201137 2 7.863460475400775 12.439877568867512 3 2.2940851299060254 5.443812855268911 4 0.9950248756218906 3.1482291211193703 5 0.4767827529021559 1.8856580673371228 6 0.28330569375345493 1.344556187144731 7 0.22802653399668327 1.2625710537822474 8 0.12437810945273632 0.7870572802798426 9 0.11055831951354339 0.7870572802798426 >10 0.30403537866224434 3.831438565806734 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 46 0.2514210756449497 No Hit ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 41 0.2240926978574552 No Hit ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 38 0.20769567118495846 No Hit ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 35 0.19129864451246176 No Hit ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 22 0.12024486226497595 No Hit ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT 22 0.12024486226497595 No Hit ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 21 0.11477918670747705 No Hit ACACCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.10931351114997813 No Hit ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 20 0.10931351114997813 No Hit ACACCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 19 0.10384783559247923 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.005465675557498907 0.0 0.0 0.0 0.0 12 0.005465675557498907 0.0 0.0 0.0 0.0 13 0.03825972890249235 0.0 0.0 0.0 0.0 14 0.04919108001749016 0.0 0.0 0.0 0.0 15 0.05465675557498907 0.0 0.0 0.0 0.0 16 0.05465675557498907 0.0 0.0 0.0 0.0 17 0.05465675557498907 0.0 0.0 0.0 0.0 18 0.05465675557498907 0.0 0.0 0.0 0.0 19 0.060122431132487976 0.0 0.0 0.0 0.0 20 0.06558810668998688 0.0 0.0 0.0 0.0 21 0.06558810668998688 0.0 0.0 0.0 0.0 22 0.07105378224748579 0.0 0.0 0.0 0.0 23 0.0765194578049847 0.0 0.0 0.0 0.0 24 0.08745080891998251 0.0 0.0 0.0 0.0 25 0.08745080891998251 0.0 0.0 0.0 0.0 26 0.08745080891998251 0.0 0.0 0.0 0.0 27 0.08745080891998251 0.0 0.0 0.0 0.0 28 0.10384783559247923 0.0 0.0 0.0 0.0 29 0.10384783559247923 0.0 0.0 0.0 0.0 30 0.10384783559247923 0.0 0.0 0.0 0.0 31 0.10384783559247923 0.0 0.0 0.0 0.0 32 0.10384783559247923 0.0 0.0 0.0 0.0 33 0.10384783559247923 0.0 0.0 0.0 0.0 34 0.10384783559247923 0.0 0.0 0.0 0.0 35 0.10384783559247923 0.0 0.0 0.0 0.0 36 0.10384783559247923 0.0 0.0 0.0 0.0 37 0.10931351114997814 0.0 0.0 0.0 0.0 38 0.10931351114997814 0.0 0.0 0.0 0.0 39 0.11477918670747704 0.0 0.0 0.0 0.0 40 0.12024486226497595 0.0 0.0 0.0 0.0 41 0.12024486226497595 0.0 0.0 0.0 0.0 42 0.12024486226497595 0.0 0.0 0.0 0.0 43 0.12571053782247485 0.0 0.0 0.0 0.0 44 0.12571053782247485 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCAGGT 15 0.007939993 50.55989 38 ACTGTTC 15 0.008117027 50.27978 45 CCAAAGT 15 0.008117027 50.27978 46 GTTCTTC 15 0.008117027 50.27978 48 GAGCCTC 15 0.008117027 50.27978 46 TTCTTCC 15 0.008117027 50.27978 49 AAGTGCA 15 0.008117027 50.27978 49 GCCAAAG 15 0.008117027 50.27978 45 AAAGTGC 15 0.008117027 50.27978 48 CTGTTCT 15 0.008117027 50.27978 46 AGCTGCA 15 0.008117027 50.27978 35 CGCCCGG 15 0.008206637 50.14089 15 GAGCTGC 15 0.008206637 50.14089 34 AGTGCAT 15 0.008206637 50.14089 50 TCGTCTC 15 0.008206637 50.14089 15 ATCGTCT 15 0.008206637 50.14089 14 TCGGCTC 15 0.0082969805 50.002758 17 ACTAGCA 15 0.0082969805 50.002758 19 GCCCGGG 15 0.0082969805 50.002758 16 TTCGGCT 15 0.0082969805 50.002758 16 >>END_MODULE