FastQCFastQC Report
Fri 17 Jun 2016
SRR1526236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63836
Sequences flagged as poor quality0
Sequence length56
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4750.7440942414938279No Hit
CGGCAGGGGTGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGG2250.35246569333918165No Hit
CGGCAGGGGTGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCGG2160.3383670656056144No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1420.22244501535183908No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1160.1817156463437559No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1150.1801491321511373No Hit
CGGCAGGGGTGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGG1140.1785826179585187No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC860.1347202205651983No Hit
CGGCAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.12218810702424965No Hit
CGGCAGGGGTGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCGG770.12062159283163106No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC700.10965599348330096No Hit
CGGCAGGGGCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCAGAG670.1049564509054452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT251.9555344E-550.46184540
CGCACCG251.9555344E-550.46184539
ACCACTG150.00809465550.4618439
GCGGTCT203.9849634E-450.42176437
TATGCTC150.00812014850.4217635
TTATTCC150.00812014850.4217635
TAAGACC251.964766E-550.4217635
ATGCTTG600.050.4217636
TATTCCC150.00812014850.4217636
CGATCCT150.00812014850.4217636
GACGATC150.0082227250.26207434
GTTATTC150.0082227250.26207434
CATTTAT204.1116722E-450.10339745
TTGATAT204.1116722E-450.10339749
TTTGATA204.1116722E-450.10339748
GTTCGCC150.00832625150.10339448
TTCGGCG252.0494881E-550.0638833
CGTTATT150.00835228450.06387733
ACCGTTC301.023096E-650.06387742
GCATATG900.050.02443332