##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526236_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 63836 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.9296478476095 39.0 38.0 39.0 35.0 40.0 2 37.173475781690584 39.0 38.0 39.0 33.0 40.0 3 37.033977692837894 39.0 38.0 39.0 32.0 40.0 4 37.25386929005577 39.0 38.0 39.0 33.0 40.0 5 36.14211416755436 38.0 36.0 39.0 30.0 40.0 6 37.058822607932825 39.0 37.0 39.0 33.0 40.0 7 37.61413622407419 39.0 38.0 39.0 35.0 40.0 8 37.47692524594273 39.0 38.0 39.0 34.0 40.0 9 37.420468074440755 39.0 38.0 39.0 33.0 40.0 10 36.759884704555425 39.0 36.0 39.0 32.0 40.0 11 35.83678488627107 38.0 35.0 39.0 29.0 40.0 12 33.90698038724231 38.0 32.0 39.0 22.0 40.0 13 33.85749420389749 38.0 33.0 39.0 22.0 40.0 14 33.99205777304342 38.0 33.0 39.0 22.0 40.0 15 33.925543580424836 38.0 33.0 39.0 22.0 40.0 16 33.39861520145372 37.0 31.0 39.0 21.0 40.0 17 33.370323955135035 37.0 31.0 39.0 21.0 40.0 18 32.84336424588007 36.0 30.0 39.0 19.0 40.0 19 33.730058274327966 37.0 31.0 39.0 23.0 40.0 20 33.739958644025315 37.0 31.0 39.0 23.0 40.0 21 34.50377529920421 38.0 33.0 39.0 25.0 40.0 22 34.0948211040792 38.0 33.0 39.0 23.0 40.0 23 34.62884579234288 38.0 33.0 39.0 26.0 40.0 24 34.200764458925995 38.0 33.0 39.0 24.0 40.0 25 34.45239363368632 38.0 33.0 39.0 25.0 40.0 26 35.114919481170496 38.0 35.0 39.0 27.0 40.0 27 34.97184973995864 38.0 34.0 39.0 27.0 40.0 28 34.940065166990415 38.0 34.0 39.0 27.0 40.0 29 34.71061783319757 38.0 34.0 39.0 27.0 40.0 30 34.563522150510686 38.0 33.0 39.0 26.0 40.0 31 34.90875054827997 38.0 35.0 39.0 27.0 40.0 32 34.567078137727925 38.0 34.0 39.0 26.0 40.0 33 34.75056394510934 38.0 34.0 39.0 27.0 40.0 34 34.68091672410552 38.0 34.0 39.0 27.0 40.0 35 34.533037784322325 38.0 34.0 39.0 27.0 40.0 36 33.78045303590451 36.0 33.0 38.0 26.0 39.0 37 33.30998182843537 36.0 32.0 38.0 25.0 39.0 38 33.236214675104954 36.0 32.0 38.0 24.0 39.0 39 32.7915752866721 35.0 32.0 38.0 22.0 39.0 40 29.305297950999435 35.0 23.0 38.0 8.0 39.0 41 26.16432733880569 31.0 15.0 37.0 5.0 39.0 42 30.308932263926312 34.0 28.0 38.0 10.0 39.0 43 28.76203082899931 33.0 25.0 38.0 4.0 39.0 44 28.72437182780876 33.0 25.0 38.0 4.0 39.0 45 29.845557365749734 34.0 28.0 38.0 4.0 39.0 46 29.492480731875432 35.0 27.0 38.0 4.0 39.0 47 29.049674165047936 34.0 26.0 38.0 4.0 39.0 48 29.145121874804186 34.0 26.0 38.0 4.0 39.0 49 26.572137978570087 33.0 14.0 38.0 4.0 39.0 50 28.674572341625414 34.0 25.0 38.0 4.0 39.0 51 28.885926436493513 35.0 26.0 39.0 4.0 40.0 52 28.66099066357541 35.0 25.0 39.0 4.0 40.0 53 28.169481170499406 34.0 23.0 38.0 4.0 39.0 54 27.247759884704557 34.0 17.0 38.0 4.0 39.0 55 27.58747415251582 34.0 20.0 38.0 4.0 39.0 56 26.47338492386741 33.0 15.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.2832809750499834 1 2 0.543268718910987 1 3 0.5224314583806375 1 4 0.6513644534007952 1 5 1.9769982319872526 1 6 0.545254610057917 1 7 -0.03838846204644142 1 8 0.09669498613346406 1 9 0.1439580725597338 1 10 -0.15063846498642164 1 11 -4.2042521742968475 1 12 -13.141750766117323 1 13 -9.114608139682485 1 14 -6.175535216969493 1 15 -6.466837841064752 1 16 -22.972903729650827 1 17 -5.427718298196144 1 18 -14.384695609719575 1 19 -6.485769657480716 1 20 -5.846405052600197 1 21 -6.329009448468344 1 22 -3.211362663860168 1 23 -3.968579636431773 1 24 -5.175309899233227 1 25 -4.124312722021628 1 26 0.9258268879127982 1 27 0.6473775118240468 1 28 0.4481199719319733 1 29 0.32493855099866664 1 30 0.6818963359266661 1 31 0.1816284964747723 1 32 0.3134434064125884 1 33 0.3589096152221174 1 34 -0.8859040867933885 1 35 -0.4393994857436496 1 36 0.6755632444005499 1 37 0.5395751090799124 1 38 0.13627998108849226 1 39 1.1677908294632644 1 40 3.9806891946505623 1 41 -10.74398760369765 1 42 -10.608534424888962 1 43 -18.12820038315741 1 44 -21.360517787367808 1 45 -13.784001035337445 1 46 -18.56331923343628 1 47 -22.829249272934085 1 48 -16.643991813825643 1 49 -19.602352661214002 1 50 -15.758887926522018 1 51 -13.308926889251607 1 52 -13.165489335207416 1 53 -12.743564350759375 1 54 -12.365979020701724 1 55 -12.790306836722529 1 56 -9.426655069560471 2 1 1.0630292588028425 2 2 1.6090581925951994 2 3 1.5704863782891039 2 4 1.7354605632406077 2 5 2.5124673395387447 2 6 1.5057809258473895 2 7 1.2053186317750715 2 8 1.4302190135934225 2 9 1.1862921686695742 2 10 1.1531372787206706 2 11 0.4650842101425141 2 12 -2.1989590041035925 2 13 -2.4201001305291676 2 14 2.016112380284511 2 15 2.26599968753937 2 16 3.3085608012416188 2 17 3.464730214389668 2 18 -1.1570068225342176 2 19 2.6578230198648214 2 20 2.8578283570107885 2 21 2.584606112172388 2 22 2.8441293269865113 2 23 2.5285599516689174 2 24 2.306955546991027 2 25 2.534726179580204 2 26 1.9509985126267537 2 27 2.474608633105511 2 28 2.261621116096734 2 29 2.193931686010103 2 30 2.6859009125857014 2 31 2.124992340868367 2 32 2.9255715528656765 2 33 2.457307784558502 2 34 1.6306863022226281 2 35 1.6218133289016592 2 36 2.413549514423437 2 37 2.4394606926039373 2 38 2.7781564112944466 2 39 3.139758792849996 2 40 6.711238393735233 2 41 -6.660463576237696 2 42 -7.362539001548001 2 43 -12.191129444942305 2 44 -14.830769503614947 2 45 -6.239950692088016 2 46 -10.655996578974037 2 47 -17.52089687018809 2 48 -8.333923163940064 2 49 -11.445602089131622 2 50 -7.3504211073000505 2 51 -1.800917735933531 2 52 -1.841118625825267 2 53 -2.403175334741068 2 54 -1.260143780427125 2 55 -1.896714159376991 2 56 1.2209421844441053 3 1 1.2345533868487095 3 2 1.6777395112765205 3 3 1.9333626324697093 3 4 1.4872522498149081 3 5 2.652099446562154 3 6 1.736550156616623 3 7 1.1115537010536158 3 8 1.3365735286435907 3 9 1.495895608707798 3 10 1.90445118220849 3 11 2.3848166516140807 3 12 3.809521645681407 3 13 2.3801865393227217 3 14 3.2045978079003774 3 15 3.356181245112232 3 16 4.491671169134598 3 17 4.835728781040409 3 18 5.013800630881931 3 19 3.8268387867066735 3 20 4.43714034936626 3 21 3.0032635416993827 3 22 3.601534964826932 3 23 3.1255499182240953 3 24 3.5324691638089902 3 25 3.547029094057031 3 26 2.4716626311169563 3 27 2.526209206445209 3 28 2.113388913516708 3 29 2.250907319072695 3 30 2.8457193550128395 3 31 2.20693214019947 3 32 1.529608819946418 3 33 2.630743044950286 3 34 1.673089551147612 3 35 2.3995247479174253 3 36 3.5851930882409206 3 37 3.5621315000573546 3 38 3.045476048179296 3 39 2.493557168387497 3 40 7.576264671804989 3 41 6.866770059691589 3 42 3.5820381604204634 3 43 -1.1315260049040852 3 44 1.7500952837715786 3 45 3.776293932852262 3 46 1.1881266890622761 3 47 -3.448393286814941 3 48 1.936979846093383 3 49 3.7179191722157263 3 50 2.310950130158144 3 51 3.076411456613055 3 52 4.142636749234459 3 53 3.3379856781590753 3 54 4.692436248239861 3 55 3.9426312777945327 3 56 4.66241375635046 4 1 1.764678434215135 4 2 2.33606968684807 4 3 2.6521955237163937 4 4 2.379888349347553 4 5 3.39215249696003 4 6 2.264401615502564 4 7 1.8528698561628758 4 8 2.0209236612695847 4 9 2.0468618838120065 4 10 1.9100088429385593 4 11 2.734949277608777 4 12 5.2247041643122 4 13 4.638870384213462 4 14 4.5076166281605765 4 15 4.726144615075597 4 16 5.955862150566958 4 17 5.542941108942394 4 18 6.276526410849087 4 19 5.3233652487503775 4 20 5.002506018180199 4 21 4.325860484985981 4 22 4.368492145577221 4 23 4.2024729951471755 4 24 4.831193428050497 4 25 4.162287398970506 4 26 3.4786097070295554 4 27 3.181255309767174 4 28 3.463395229040266 4 29 3.434436433636293 4 30 3.360813456313842 4 31 3.036286693691956 4 32 3.463927374954629 4 33 3.23791747971093 4 34 2.289232029359056 4 35 2.850074198466878 4 36 4.575593492434429 4 37 4.287911467216631 4 38 4.75092002948535 4 39 4.8636468488220075 4 40 9.154766629617288 4 41 11.717597393277543 4 42 7.692433512195127 4 43 9.373463258705872 4 44 8.011684269498495 4 45 7.201328668231824 4 46 7.3333837308710415 4 47 7.3295529049243555 4 48 7.324247750602666 4 49 9.834882668489566 4 50 7.683187088602001 4 51 6.637987811292856 4 52 7.878521554084777 4 53 7.622436859161979 4 54 8.147027633865726 4 55 7.734345310887875 4 56 8.058523393839405 5 1 1.3508853307668574 5 2 1.6923915259285351 5 3 1.686678282337084 5 4 1.7936814527958305 5 5 2.782957094661178 5 6 1.7701487419393445 5 7 1.0827549136341403 5 8 1.4347167647178622 5 9 1.64456303323729 5 10 1.5651812567316625 5 11 2.286673415539809 5 12 4.2247041643122 5 13 4.098640499155991 5 14 3.5880763982755184 5 15 3.87556990243192 5 16 4.760459851716384 5 17 4.336044557218251 5 18 4.655836755676674 5 19 4.00152616829061 5 20 3.899057742318135 5 21 3.0499984160204647 5 22 4.138607088105957 5 23 2.6392546043425753 5 24 3.647285382073484 5 25 2.7714828012693573 5 26 1.9498740748456456 5 27 2.261715079882123 5 28 1.7507515508793432 5 29 2.445930686509854 5 30 1.7171352953943 5 31 1.783413130473562 5 32 2.2110538117362424 5 33 1.5712508130442586 5 34 1.57658835119814 5 35 2.0914535088117034 5 36 3.3457084349631643 5 37 3.299405720090199 5 38 2.65896600649684 5 39 3.5877847798564915 5 40 7.499594215824185 5 41 9.464723830059157 5 42 5.968295581160643 5 43 7.72978509778633 5 44 7.1151325453605665 5 45 5.94845510501343 5 46 6.103498673399777 5 47 6.777828766993316 5 48 6.3702247620969175 5 49 8.972813702972328 5 50 7.303876743774413 5 51 6.270171719338837 5 52 6.211854887418113 5 53 6.070712721230947 5 54 6.238981656854236 5 55 5.7918165752556945 5 56 4.966569370850902 6 1 1.3185311374931246 6 2 1.9146137481507566 6 3 2.0919571243975312 6 4 1.9890837516464046 6 5 2.9540937485564527 6 6 2.1847932715264022 6 7 1.2441001669334142 6 8 1.4087482674849952 6 9 0.8642309770431709 6 10 1.4502387279960303 6 11 2.645975246106012 6 12 4.325598161757924 6 13 4.432825258628107 6 14 4.530605133907699 6 15 4.577996466927452 6 16 5.2010728785363085 6 17 4.6050952170735115 6 18 4.892107509188811 6 19 4.116894410095632 6 20 3.63426421315679 6 21 2.363323235092409 6 22 2.6486113452366453 6 23 1.8814853408261882 6 24 2.9950929597661187 6 25 2.9336794807074185 6 26 1.2248847176723814 6 27 1.9517959653653065 6 28 2.2198873533484758 6 29 2.2684083365737138 6 30 1.3399535159136704 6 31 1.0758780091453346 6 32 1.6742296312338993 6 33 1.0327237802643552 6 34 1.253472131530195 6 35 1.6772346922940358 6 36 2.4853423217234933 6 37 1.9801209180467723 6 38 2.7854027881060404 6 39 2.8083041498011454 6 40 6.7098964721034555 6 41 3.00325086283906 6 42 5.150075061790698 6 43 6.397303190591781 6 44 5.162386696062995 6 45 5.096603253161582 6 46 4.862119363054951 6 47 5.5355980305097106 6 48 4.878100453880656 6 49 6.896185350481904 6 50 5.341339493880842 6 51 4.584347964549561 6 52 4.624796564727607 6 53 5.015370022210085 6 54 5.35136990717352 6 55 4.512974514804437 6 56 5.182831610101648 7 1 1.5043336066289257 7 2 2.0340581925951966 7 3 1.761247247854321 7 4 1.7474170849797375 7 5 2.7934456004083046 7 6 1.8682809258473867 7 7 1.1879273274272464 7 8 1.5519581440282053 7 9 1.6248791251913133 7 10 1.1710720613293688 7 11 2.244432036229469 7 12 4.566801865461628 7 13 4.0413129129490954 7 14 4.038938467241032 7 15 4.7719779484089315 7 16 5.120517322980753 7 17 4.578860649172277 7 18 5.112014916596216 7 19 4.277388237256126 7 20 3.615437052662962 7 21 3.5617800252158673 7 22 4.245216283508256 7 23 2.5899729951471784 7 24 3.810216416556244 7 25 2.627660962188898 7 26 1.7955637300180598 7 27 1.2789564591924645 7 28 2.525751550879342 7 29 1.9716490773144528 7 30 2.11742265171614 7 31 2.0684706017379284 7 32 1.8206802485178457 7 33 1.412199088906327 7 34 1.6219906500487156 7 35 1.525074198466875 7 36 1.7189842970321294 7 37 2.576417214343067 7 38 1.7645694547727047 7 39 2.769106618936952 7 40 5.781347089387406 7 41 0.2862755541970863 7 42 4.730939259321563 7 43 6.334957511579436 7 44 5.960534844211139 7 45 4.694288438346767 7 46 5.728786029721618 7 47 5.931277042855385 7 48 5.976402923016458 7 49 8.548963128259683 7 50 6.052296284004296 7 51 6.724625742327337 7 52 6.812142243739949 7 53 6.388672491345886 7 54 6.655073610877224 7 55 5.9853510580143165 7 56 6.122029140965843 8 1 1.2444570634190555 8 2 1.7294285629655732 8 3 1.943808976249386 8 4 1.7792072084365316 8 5 2.6701431312725035 8 6 1.9378796912794911 8 7 1.1082976977976173 8 8 0.9025754279788245 8 9 1.419786532598721 8 10 1.820609098366404 8 11 2.3867159868467525 8 12 4.226832729659158 8 13 4.309368468504651 8 14 3.75282735612992 8 15 4.602687824952142 8 16 4.707245718042479 8 17 4.765589044234005 8 18 5.015564299312267 8 19 3.3020795952808157 8 20 3.362659274885182 8 21 3.091718296820801 8 22 3.4881175180761517 8 23 3.1407446000854478 8 24 3.4025003671735305 8 25 3.291704172065444 8 26 2.138464964585957 8 27 2.075252755488762 8 28 1.849516982978109 8 29 2.5276675958329733 8 30 2.673286849247006 8 31 2.43390270050336 8 32 2.6865753102462406 8 33 2.378402792610032 8 34 2.290509168567233 8 35 1.6895803713063842 8 36 2.3125028155506513 8 37 1.1653061032319556 8 38 1.6496003189702364 8 39 1.832995507825835 8 40 5.3642174597577785 8 41 -3.0708232112350125 8 42 2.9155071605561353 8 43 4.792364918986838 8 44 4.520411387421014 8 45 3.4793193025442974 8 46 4.4917489926845775 8 47 4.5602893885344 8 48 4.470693046473251 8 49 6.73569152332141 8 50 3.7364012222759015 8 51 5.559656606524872 8 52 4.353191626456002 8 53 5.225246565419965 8 54 4.314332870136482 8 55 4.414209082705671 8 56 2.1828316101016476 9 1 1.2465413988367189 9 2 1.9564607899977986 9 3 2.1461498452569217 9 4 2.2185209810836355 9 5 2.494257288719993 9 6 2.0901965102629774 9 7 1.2574078469077605 9 8 1.6662438583139192 9 9 1.6227687355809266 9 10 1.601753879511186 9 11 2.8863151531125837 9 12 4.081249917409679 9 13 4.701864861001042 9 14 4.591211194513761 9 15 5.0075298964608805 9 16 4.749413426876856 9 17 4.976587921899551 9 18 5.143670760752059 9 19 4.4072583671262535 9 20 5.179398091624002 9 21 4.071195609631452 9 22 4.218917582209549 9 23 3.6050703977445764 9 24 3.980021611361444 9 25 3.7138622608901954 9 26 2.7653689248232567 9 27 2.7140213942574007 9 28 2.711790511918302 9 29 2.853304921470297 9 30 3.415149924443412 9 31 2.161977095244424 9 32 3.165972456310058 9 33 2.233140647347888 9 34 1.7979646760227368 9 35 2.0318923802850577 9 36 2.9887894918373306 9 37 2.9257678636937214 9 38 3.088433091136338 9 39 3.294755969586298 9 40 6.188652284192603 9 41 2.4911456840672166 9 42 5.044575622957932 9 43 5.9174250440469685 9 44 5.687158220834519 9 45 4.931139087697417 9 46 5.736578237513825 9 47 5.868290029868376 9 48 5.221695130808669 9 49 8.284352738649293 9 50 5.33492615413417 9 51 5.491995872197467 9 52 6.325453932051637 9 53 5.320003660177058 9 54 6.171956727760339 9 55 5.021552356715613 9 56 4.4496265435632445 10 1 1.2011690496669019 10 2 1.9919695850002626 10 3 2.055076361778376 10 4 2.1958348064987305 10 5 3.5654392712943803 10 6 1.8602113055942269 10 7 1.510079226161423 10 8 1.648160675673772 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2.7168264529997757 115 23 2.0329814697234454 115 24 2.2767418402850623 115 25 2.4660084198160206 115 26 1.7400552554417885 115 27 2.151837815124665 115 28 1.7338023983369695 115 29 1.4667761959585235 115 30 1.8034396008686784 115 31 1.956606194958269 115 32 2.1446209264839524 115 33 0.6746990889063298 115 34 0.958431328014818 115 35 1.3246504696533208 115 36 2.505848703811793 115 37 1.9543833160379833 115 38 2.5707135225693136 115 39 2.9271574663945756 115 40 7.570118275828083 115 41 3.6663602999597984 115 42 0.01992231016902224 115 43 -8.360805200284975 115 44 -6.825058376127842 115 45 -0.1419403752125561 115 46 -4.844095326210589 115 47 -3.6949941435852907 115 48 -3.6471140261360837 115 49 -13.4616300920793 115 50 -3.39961049565672 115 51 -2.756518325469269 115 52 -2.0408238579549653 115 53 -3.0691664917049586 115 54 -6.131367067088878 115 55 -1.8534201284263645 115 56 0.04702914096584365 116 1 0.43940823349460345 116 2 1.2396552075205705 116 3 1.0860606806901458 116 4 0.904506637218546 116 5 2.507438137721742 116 6 1.5207062989817146 116 7 0.4841959841436605 116 8 0.23554023358044418 116 9 0.48999106548982496 116 10 1.4701392255084684 116 11 -4.08131423242725 116 12 -14.582638433045837 116 13 -9.947679624364337 116 14 -5.225613771564937 116 15 -5.985298170994053 116 16 -5.51866178149686 116 17 -3.14688561948444 116 18 -10.4467537401202 116 19 -3.468880419460291 116 20 -4.456577872710174 116 21 1.3823024132755677 116 22 1.7858879252992992 116 23 0.9785923981322497 116 24 1.710589550884606 116 25 1.1315788726366591 116 26 0.7347428344956697 116 27 1.8237325785954468 116 28 0.5417963269987496 116 29 1.1584028086577405 116 30 1.7245122039549443 116 31 0.563992989797633 116 32 1.137284726129792 116 33 0.7642513277123015 116 34 -0.630994424578148 116 35 0.3575368850340439 116 36 1.4208499686739202 116 37 0.9331336322535151 116 38 0.8849052756682241 116 39 1.8097782607279953 116 40 5.366981417745617 116 41 1.2269471959881315 116 42 -4.975777158588883 116 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0.6151609621889023 117 26 1.970563730018057 117 27 1.8389564591924668 117 28 1.470751550879342 117 29 2.0091490773144542 117 30 2.3624226517161375 117 31 0.7884706017379273 117 32 0.9781802485178517 117 33 1.6146990889063275 117 34 0.5719906500487113 117 35 0.890074198466877 117 36 1.2739842970321291 117 37 1.5964172143430702 117 38 2.1720694547727035 117 39 1.364106618936951 117 40 5.733847089387407 117 41 2.381275554197085 117 42 -2.499060740678434 117 43 -10.395042488420568 117 44 -9.409465155788858 117 45 -1.5782115616532337 117 46 -3.071213970278386 117 47 -3.4387229571446127 117 48 -3.8260970769835403 117 49 -8.286036871740318 117 50 -3.1352037159957042 117 51 -3.0378742576726587 117 52 -3.3028577562600496 117 53 -4.588827508654109 117 54 -8.384926389122779 117 55 -2.4771489419856856 117 56 -1.252970859034157 118 1 0.2090505877610056 118 2 0.9816997020291609 118 3 1.2468604554014888 118 4 0.8507189717721957 118 5 1.8814172985215123 118 6 1.2416299824511654 118 7 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1.6443410745770777 119 28 1.0776746278024234 119 29 1.2783798465452207 119 30 1.9308841901776788 119 31 0.5530859863533166 119 32 -5.349512059174458 119 33 -1.0176086034013636 119 34 -1.5741631961051326 119 35 -1.7076181092254288 119 36 -0.14832339527556115 119 37 0.33410952203537647 119 38 0.5097617624650113 119 39 0.32872200355233616 119 40 3.186154781695098 119 41 0.6689678618893922 119 42 -4.8190607406784345 119 43 -11.115042488420567 119 44 -9.381772848096553 119 45 -7.718211561653234 119 46 -18.240444739509154 119 47 -15.503338341759997 119 48 -15.274558615445079 119 49 -14.24911379481724 119 50 -14.515972946764933 119 51 -13.176335796134198 119 52 -13.05516544856774 119 53 -13.90651981634642 119 54 -14.541080235276624 119 55 -12.373302788139533 119 56 -10.068355474418771 120 1 -0.7251535728582539 120 2 -0.28196744843044286 120 3 0.010285709392782394 120 4 -0.08783932527666849 120 5 1.2674840619467673 120 6 0.19808861815508294 120 7 -0.41591882641890976 120 8 -0.4070162149461538 120 9 -0.7312106183984284 120 10 -0.24206896431166314 120 11 -4.2305679637705325 120 12 -7.868134031974272 120 13 -7.887212728076548 120 14 -12.930933327630761 120 15 -10.786675897744914 120 16 -17.072431394967964 120 17 -16.091011145699518 120 18 -15.879972262890963 120 19 -6.209791249923359 120 20 -12.3211014088755 120 21 -6.333732795296957 120 22 -3.566963203671232 120 23 -3.4641936715194923 120 24 -8.77888614754632 120 25 -3.610480063452126 120 26 1.1346662941206205 120 27 -0.8620691818331778 120 28 0.3148541149819053 120 29 0.36812343628881194 120 30 0.8860123953058832 120 31 -1.8699909367236103 120 32 -12.093101802764203 120 33 -0.9150445008372614 120 34 -1.5100606320025705 120 35 -1.58582323743056 120 36 -0.9496054465576123 120 37 0.14821208613793857 120 38 -0.624853622150372 120 39 0.48256815739848946 120 40 3.9425650381053536 120 41 -2.2797500868285567 120 42 -5.883163304781 120 43 -12.71760659098467 120 44 -10.792029258352962 120 45 -12.128467971909643 120 46 -16.49685499591941 120 47 -16.96487680329846 120 48 -13.524558615445079 120 49 -17.172190717894164 120 50 -13.073665254457241 120 51 -11.63787425767266 120 52 -11.542344935747227 120 53 -11.874468534295136 120 54 -14.201336645533035 120 55 -12.046379711216456 120 56 -9.46579137185467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 195.0 5 16.0 6 81.0 7 69.0 8 87.0 9 114.0 10 105.0 11 82.0 12 80.0 13 95.0 14 120.0 15 156.0 16 238.0 17 290.0 18 351.0 19 415.0 20 517.0 21 684.0 22 825.0 23 944.0 24 1089.0 25 1313.0 26 1469.0 27 1605.0 28 1875.0 29 2186.0 30 2609.0 31 3057.0 32 3530.0 33 4031.0 34 4628.0 35 5587.0 36 7155.0 37 8198.0 38 6784.0 39 3251.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.1650479353343 0.6845667021743217 0.08145873801616643 0.06892662447521775 8 98.74522213171252 1.0104016542389875 0.15978444764709568 0.0845917664014036 9 94.9761889842722 3.2191866658311925 0.9618397142678113 0.8427846356287988 10 40.35810514443261 35.248135848110785 16.15546086847547 8.238298138981138 11 35.2152390500658 31.746976627608248 15.44426342502663 17.59352089729933 12 33.81007581928692 19.008083213233913 21.019487436556176 26.16235353092299 13 27.14838547949927 15.474642392717817 23.84727466432701 33.5296974634559 14 23.46168306284855 23.532176201516386 27.782129206090605 25.22401152954446 15 29.71585557299843 16.500784929356357 29.532182103610676 24.25117739403454 16 34.2579967715141 19.138965944175403 23.13852712084881 23.46451016346169 17 30.249389059464875 21.812143618021178 23.740522589134656 24.197944733379284 18 26.159220502537757 26.21718152766464 23.464816091233786 24.158781878563822 19 27.902674568087043 24.604145110212272 20.78982848901013 26.703351832690558 20 26.243414450577017 25.122303060712493 25.269693928750627 23.36458855995986 21 26.48035591202456 21.298326962842285 27.6380099003697 24.583307224763455 22 25.32113540948681 25.350899179146563 23.50554546024187 25.822419951124758 23 26.65110595901999 25.88664703302212 23.624600538880884 23.837646469077008 24 24.884467768465576 27.18101355056004 26.1799953003838 21.754523380590584 25 29.40439264341887 21.86139048156155 26.45612056270953 22.27809631231005 26 26.840043866520446 21.256462478458403 28.134106219645933 23.769387435375215 27 21.784056698444555 22.655858003010536 30.718452082288007 24.841633216256902 28 21.447616060225847 26.12296110414053 30.52854454203262 21.900878293601004 29 23.898326333604963 20.587036920955306 35.91330784178524 19.601328903654487 30 20.527041423826535 19.397443128407595 41.30475653318293 18.77075891458294 31 24.108751860847764 21.291232468855284 36.137271801300635 18.462743868996316 32 23.306207566991237 21.236495915455414 37.81770857832761 17.63958793922574 33 20.837517258692102 17.585038282917033 39.310593699008415 22.266850759382454 34 21.805359661495064 20.52499608211879 42.67983074753173 14.98981350885441 35 21.435624167385 20.023509129378574 44.50121463835123 14.039652064885196 36 20.734612009527392 22.500626802055912 41.607120471355145 15.157640717061552 37 18.85907093534212 19.609227871939737 46.26647834274953 15.265222849968612 38 20.331422288541585 23.27809628524307 41.07437367470352 15.31610775151182 39 18.27199246645217 19.29059091265793 40.84438515263282 21.59303146825708 40 16.348610541589125 14.508090716966848 38.38280288931647 30.760495852127555 41 12.327187119811663 14.817272597999715 35.82183880804538 37.03370147414324 42 18.013990840077795 21.486605182257357 42.77401342618734 17.725390551477506 43 13.909780338834022 16.021604986732402 44.451946175948756 25.616668498484824 44 15.113314447592069 18.947119924457034 41.301542335536666 24.63802329241423 45 20.039240307644015 18.41469157118192 44.59268560665516 16.953382514518914 46 16.547643338094268 19.10756046646681 45.13521573305291 19.20958046238601 47 15.184378379225501 20.628751430049054 46.504411603378834 17.682458587346613 48 17.90487544707285 20.912028612662358 42.465645981050386 18.71744995921441 49 14.237362844953797 17.612518300458103 40.977283818459455 27.172835036128646 50 20.78614675157637 20.019136054208364 42.37694889732409 16.817768296891174 51 16.15898201241065 25.668054355510172 42.405152776686826 15.76781085539235 52 19.252242644752524 20.408067247976916 44.00915877297535 16.330531334295216 53 16.091376929835572 21.005397263712815 46.895004393121624 16.008221413329988 54 15.598260405382067 21.41993625673151 39.511406276984914 23.47039706090151 55 19.978335609664203 22.70836276864629 40.862493916701986 16.45080770498752 56 21.914669930486905 21.775016083729543 39.44828884809113 16.862025137692417 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 2.0 10 2.0 11 2.0 12 12.0 13 36.0 14 50.0 15 90.5 16 131.0 17 162.0 18 193.0 19 279.0 20 365.0 21 447.0 22 657.0 23 785.0 24 882.0 25 979.0 26 1168.0 27 1357.0 28 1551.5 29 2076.5 30 2407.0 31 2633.5 32 2860.0 33 3050.5 34 3241.0 35 3145.0 36 3049.0 37 2658.5 38 1889.0 39 1510.0 40 1302.0 41 1094.0 42 1028.5 43 963.0 44 933.5 45 904.0 46 933.0 47 1053.5 48 1145.0 49 1483.5 50 1822.0 51 1934.0 52 2046.0 53 2171.0 54 2357.5 55 2419.0 56 2467.0 57 2515.0 58 2522.0 59 2529.0 60 2610.0 61 2691.0 62 2900.0 63 3288.0 64 3467.0 65 3482.5 66 3498.0 67 3198.5 68 2899.0 69 2636.5 70 2374.0 71 2068.0 72 1558.0 73 1354.0 74 1164.0 75 974.0 76 811.5 77 649.0 78 546.0 79 336.0 80 229.0 81 170.0 82 111.0 83 81.0 84 51.0 85 32.5 86 14.0 87 12.5 88 9.0 89 7.0 90 4.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.014098627733567267 14 0.0 15 0.21304593019612758 16 0.04542891158593897 17 0.0 18 0.0 19 0.07832570963092926 20 0.0939908515571151 21 0.0 22 0.0 23 0.0 24 0.0015665141926185851 25 0.0031330283852371702 26 0.009399085155711511 27 0.0939908515571151 28 0.11905507863901248 29 0.037596340622846044 30 0.012532113540948681 31 0.03289679804499029 32 0.09242433736449653 33 0.15665141926185852 34 0.040729369008083216 35 0.04856193997117614 36 0.0313302838523717 37 0.1817156463437559 38 0.26787392693777806 39 0.1895482173068488 40 0.6736011028259916 41 0.8537502349771289 42 0.1253211354094868 43 0.23027758631493203 44 0.4636882010151012 45 0.1973807882699417 46 0.19268124569208597 47 0.0422958832007018 48 0.1378532489504355 49 0.49031894228961714 50 0.128454163794724 51 0.2835390688639639 52 0.11278902186853813 53 0.15665141926185852 54 0.2240115295444577 55 0.21461244438874616 56 0.1676170186101886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 63836.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.95826806190864 #Duplication Level Percentage of deduplicated Percentage of total 1 86.56909260357536 64.02500156651419 2 7.898415657036346 11.683062848549408 3 2.529018046259426 5.611253837959771 4 1.078115733288147 3.1894228961714393 5 0.5528255528255528 2.0443010213672537 6 0.3621960518512243 1.6072435616266683 7 0.2096924510717614 1.0855943354846793 8 0.18003897314242143 1.0652296509806378 9 0.11861391171736 0.7895231530797668 >10 0.45751080233838853 5.326148254903189 >50 0.02965347792934 1.3753994611191178 >100 0.01482673896467 2.197819412243875 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 475 0.7440942414938279 No Hit CGGCAGGGGTGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGG 225 0.35246569333918165 No Hit CGGCAGGGGTGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCGG 216 0.3383670656056144 No Hit CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 142 0.22244501535183908 No Hit CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 116 0.1817156463437559 No Hit CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 115 0.1801491321511373 No Hit CGGCAGGGGTGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGG 114 0.1785826179585187 No Hit CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 86 0.1347202205651983 No Hit CGGCAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 78 0.12218810702424965 No Hit CGGCAGGGGTGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCGG 77 0.12062159283163106 No Hit CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 70 0.10965599348330096 No Hit CGGCAGGGGCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCAGAG 67 0.1049564509054452 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0046995425778557556 0.0 0.0 0.0 0.0 6 0.0046995425778557556 0.0 0.0 0.0 0.0 7 0.0046995425778557556 0.0 0.0 0.0 0.0 8 0.0046995425778557556 0.0 0.0 0.0 0.0 9 0.0062660567704743405 0.0 0.0 0.0 0.0 10 0.012532113540948681 0.0 0.0 0.0 0.0 11 0.01566514192618585 0.0 0.0 0.0 0.0 12 0.029763769659753116 0.0 0.0 0.0 0.0 13 0.03289679804499029 0.0 0.0 0.0 0.0 14 0.040729369008083216 0.0 0.0 0.0 0.0 15 0.045428911585938966 0.0 0.0 0.0 0.0 16 0.04699542577855755 0.0 0.0 0.0 0.0 17 0.050128454163794724 0.0 0.0 0.0 0.0 18 0.05482799674165048 0.0 0.0 0.0 0.0 19 0.05952753931950623 0.0 0.0 0.0 0.0 20 0.06579359608998057 0.0 0.0 0.0 0.0 21 0.0736261670530735 0.0 0.0 0.0 0.0 22 0.08145873801616643 0.0 0.0 0.0 0.0 23 0.09242433736449653 0.0 0.0 0.0 0.0 24 0.0955573657497337 0.0 0.0 0.0 0.0 25 0.10338993671282662 0.0 0.0 0.0 0.0 26 0.10652296509806379 0.0 0.0 0.0 0.0 27 0.11278902186853813 0.0 0.0 0.0 0.0 28 0.12062159283163106 0.0 0.0 0.0 0.0 29 0.13158719217996115 0.0 0.0 0.0 0.0 30 0.13315370637257973 0.0 0.0 0.0 0.0 31 0.1378532489504355 0.0 0.0 0.0 0.0 32 0.13941976314305407 0.0 0.0 0.0 0.0 33 0.13941976314305407 0.0 0.0 0.0 0.0 34 0.13941976314305407 0.0 0.0 0.0 0.0 35 0.1488188482987656 0.0 0.0 0.0 0.0 36 0.15038536249138418 0.0 0.0 0.0 0.0 37 0.15038536249138418 0.0 0.0 0.0 0.0 38 0.15195187668400276 0.0 0.0 0.0 0.0 39 0.15195187668400276 0.0 0.0 0.0 0.0 40 0.15508490506923991 0.0 0.0 0.0 0.0 41 0.1582179334544771 0.0 0.0 0.0 0.0 42 0.16605050441757002 0.0 0.0 0.0 0.0 43 0.1691835328028072 0.0 0.0 0.0 0.0 44 0.17231656118804436 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACCGT 25 1.9555344E-5 50.461845 40 CGCACCG 25 1.9555344E-5 50.461845 39 ACCACTG 15 0.008094655 50.46184 39 GCGGTCT 20 3.9849634E-4 50.421764 37 TATGCTC 15 0.008120148 50.42176 35 TTATTCC 15 0.008120148 50.42176 35 TAAGACC 25 1.964766E-5 50.42176 35 ATGCTTG 60 0.0 50.42176 36 TATTCCC 15 0.008120148 50.42176 36 CGATCCT 15 0.008120148 50.42176 36 GACGATC 15 0.00822272 50.262074 34 GTTATTC 15 0.00822272 50.262074 34 CATTTAT 20 4.1116722E-4 50.103397 45 TTGATAT 20 4.1116722E-4 50.103397 49 TTTGATA 20 4.1116722E-4 50.103397 48 GTTCGCC 15 0.008326251 50.103394 48 TTCGGCG 25 2.0494881E-5 50.06388 33 CGTTATT 15 0.008352284 50.063877 33 ACCGTTC 30 1.023096E-6 50.063877 42 GCATATG 90 0.0 50.024433 32 >>END_MODULE