FastQCFastQC Report
Fri 17 Jun 2016
SRR1526235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93902
Sequences flagged as poor quality0
Sequence length56
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG16701.7784498732721348No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATGAG4230.45046963855934913No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG4030.42917083768183856No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG3820.40680709676045235No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3810.4057421567165769No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGACACACGCAGGGATGAG1710.1821047475027156No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG1500.15974100658132948No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCGACACGCAGGGATGAG1450.1544163063619518No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG1230.13098762539669018No Hit
ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.12033822495793488No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCC150.00816570650.36940838
GCGACAC451.2551027E-1050.31522439
CGTCTCA550.050.31522441
CCACACG451.2551027E-1050.31522440
ACGCCCA150.008200549550.3152239
CTAGTCT150.008200549550.3152239
TCGTCTC402.4938345E-950.3152240
AGTCTCA204.0367898E-450.3152241
TGATACA204.058286E-450.2611534
CAAGAGA150.00823550350.26114736
ATACAAA150.00823550350.26114736
CAACGAT850.050.1802646
CGATTAA750.050.1802649
CCTAGTT150.00828814250.18025648
TCCTAGT150.00828814250.18025647
CTAGTTT150.00828814250.18025649
CGGATGA150.00828814250.18025649
CACTAAA150.00828814250.18025647
CTCAACG850.050.12647644
ATTCAAG150.00832337350.12647233