##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526235_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 93902 Sequences flagged as poor quality 0 Sequence length 56 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.74927051606995 39.0 38.0 40.0 35.0 40.0 2 37.163021021916464 39.0 38.0 40.0 33.0 40.0 3 37.28414730250687 39.0 38.0 40.0 33.0 40.0 4 37.25786458222402 39.0 38.0 40.0 33.0 40.0 5 37.16903793316436 39.0 38.0 40.0 33.0 40.0 6 36.810025345573045 39.0 38.0 40.0 32.0 40.0 7 37.12075355157505 39.0 38.0 40.0 33.0 40.0 8 37.05021192306873 39.0 38.0 40.0 33.0 40.0 9 37.04389682860855 39.0 38.0 40.0 33.0 40.0 10 36.73896189644523 39.0 37.0 40.0 31.0 40.0 11 36.402802922195484 38.0 36.0 40.0 31.0 40.0 12 34.28389171689634 38.0 33.0 39.0 21.0 40.0 13 34.06934889565717 38.0 33.0 39.0 20.0 40.0 14 34.11396988349556 38.0 33.0 39.0 21.0 40.0 15 34.04567527848182 38.0 33.0 39.0 20.0 40.0 16 33.35304892334562 38.0 31.0 39.0 19.0 40.0 17 33.439532704308746 38.0 31.0 39.0 20.0 40.0 18 32.9561457689932 37.0 30.0 39.0 18.0 40.0 19 33.7854465293604 38.0 31.0 39.0 23.0 40.0 20 33.59960384230368 37.0 31.0 39.0 23.0 40.0 21 34.608890119486276 38.0 33.0 39.0 25.0 40.0 22 34.351685800089456 38.0 33.0 39.0 24.0 40.0 23 34.716843091733935 38.0 34.0 39.0 25.0 40.0 24 34.32576515942152 38.0 33.0 39.0 23.0 40.0 25 34.489308001959486 38.0 33.0 39.0 25.0 40.0 26 35.12415071031501 38.0 35.0 39.0 27.0 40.0 27 35.01496240761645 38.0 35.0 39.0 27.0 40.0 28 34.8885753232093 38.0 35.0 39.0 27.0 40.0 29 34.765180720325446 38.0 34.0 39.0 26.0 40.0 30 34.61874081489212 38.0 34.0 39.0 26.0 40.0 31 35.03130923728994 38.0 35.0 39.0 28.0 40.0 32 34.596227982364596 38.0 34.0 39.0 26.0 40.0 33 34.77333816106153 38.0 35.0 39.0 27.0 40.0 34 34.728046260995505 38.0 35.0 39.0 27.0 40.0 35 34.5331941811676 38.0 34.0 39.0 26.0 40.0 36 33.83150518625801 37.0 33.0 39.0 26.0 40.0 37 33.63028476496773 37.0 33.0 39.0 25.0 40.0 38 33.40837255862495 37.0 32.0 39.0 24.0 40.0 39 33.14805861430001 36.0 32.0 39.0 23.0 40.0 40 29.781090924580948 35.0 24.0 38.0 8.0 40.0 41 26.37005601584631 32.0 15.0 38.0 4.0 39.0 42 30.67488445400524 35.0 29.0 38.0 7.0 39.0 43 29.08008349129944 34.0 26.0 38.0 4.0 39.0 44 29.136184532810802 34.0 26.0 38.0 4.0 39.0 45 30.354007369385105 35.0 29.0 38.0 4.0 39.0 46 29.975154948776385 35.0 27.0 38.0 4.0 40.0 47 29.48708227726779 35.0 27.0 38.0 4.0 40.0 48 29.57913569466039 35.0 27.0 38.0 4.0 40.0 49 27.002278971693894 33.0 15.0 38.0 4.0 40.0 50 29.24275308300143 35.0 26.0 38.0 4.0 40.0 51 29.499382334774552 35.0 27.0 39.0 4.0 40.0 52 29.128186833081298 35.0 26.0 39.0 4.0 40.0 53 28.801516474622478 35.0 25.0 39.0 4.0 40.0 54 27.80160166982599 35.0 18.0 39.0 4.0 40.0 55 28.1298907371515 35.0 23.0 39.0 4.0 40.0 56 27.22817405380077 34.0 17.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.0162320083222554 1 2 1.8567191791883317 1 3 1.6463849764737546 1 4 1.5845278263027538 1 5 1.7097077093132995 1 6 2.282096939241967 1 7 1.7777600401330602 1 8 1.9167516623468117 1 9 1.8487006228510694 1 10 1.9324118094299223 1 11 -1.804785214040166 1 12 -12.229343987571678 1 13 -9.583690447834073 1 14 -4.1815645133531625 1 15 -5.384143660285524 1 16 -22.358380085732662 1 17 -3.874974023707299 1 18 -12.976950352017909 1 19 -4.586143674307262 1 20 -4.5834141027769775 1 21 -3.384657421463828 1 22 -1.236520615545217 1 23 -1.5542546141183706 1 24 -2.035874706811761 1 25 -1.3694832372003916 1 26 3.005242549832829 1 27 3.015456116528405 1 28 3.1542452847028883 1 29 3.2827216096243603 1 30 3.4340479587869623 1 31 2.8483754460706194 1 32 3.1762637483898644 1 33 2.9811856017809717 1 34 2.337768238935311 1 35 2.827359557279138 1 36 3.1524989228971734 1 37 3.1215161100600213 1 38 3.075934978764799 1 39 3.548095505884085 1 40 7.3999237646954725 1 41 -8.226631168662529 1 42 -7.73320141913241 1 43 -15.507635278821466 1 44 -20.955278368128305 1 45 -8.675038560973608 1 46 -16.59546521978575 1 47 -22.591769734615852 1 48 -13.27656722421014 1 49 -19.255887170905112 1 50 -12.401004397234805 1 51 -8.599572369708508 1 52 -8.25339705398795 1 53 -8.123581561084404 1 54 -7.888905954736728 1 55 -8.255588933398919 1 56 -2.5184829497742562 2 1 1.4845692266594739 2 2 2.2916453641145154 2 3 2.0778146579034384 2 4 2.359974601749528 2 5 2.0275010271066165 2 6 2.6962383533833787 2 7 2.2954368078098213 2 8 2.5136825092776576 2 9 2.4724379965884395 2 10 2.124913751931871 2 11 1.9910578318028769 2 12 -0.3194760777037686 2 13 0.8073351931915695 2 14 3.992095160306512 2 15 3.5336885075466427 2 16 3.571689844337264 2 17 4.302959154225881 2 18 -0.6393435144110704 2 19 3.251463163299576 2 20 3.5057467363838626 2 21 3.4177901309837253 2 22 3.3920730130484102 2 23 3.1660251061613494 2 24 2.077373156436103 2 25 3.2503691326519757 2 26 2.7148385094287875 2 27 2.9449432960155875 2 28 2.9131807936383964 2 29 3.2028847797875315 2 30 3.377132651871655 2 31 2.3782899759851475 2 32 3.013482085608203 2 33 2.6251249957203626 2 34 2.3719562731233452 2 35 2.717802447722029 2 36 3.6579379283361817 2 37 3.6380273765712943 2 38 3.4892993921292117 2 39 3.4610714188600014 2 40 5.9127442775159835 2 41 -4.711673903705265 2 42 -4.363737549668542 2 43 -11.609033880220068 2 44 -13.296381709231646 2 45 -4.733896369831417 2 46 -9.577982702303235 2 47 -17.023976416822535 2 48 -7.251314698957614 2 49 -12.11175352677147 2 50 -6.482006728237135 2 51 -1.8635583836945244 2 52 -1.0880901386810322 2 53 -1.6985621360649787 2 54 -0.5590691248998994 2 55 -0.9871351649451512 2 56 3.007546576255269 3 1 1.7055785323144477 3 2 2.6174029398720933 3 3 2.5962470129499664 3 4 2.502422704827552 3 5 2.076892580435178 3 6 2.9102684178071456 3 7 2.721767039258168 3 8 2.592840228199151 3 9 2.595857228275385 3 10 2.483121816832849 3 11 2.8595259778300814 3 12 4.545472621890603 3 13 3.542899497653508 3 14 4.867900457371647 3 15 3.2150891279236404 3 16 5.1732169261793075 3 17 4.718134530031179 3 18 5.485328401599425 3 19 4.550675761724776 3 20 4.562356137481451 3 21 3.2921041371874935 3 22 4.04943402474013 3 23 4.112815848227676 3 24 3.4809164635227177 3 25 3.5403357277366823 3 26 2.885382532335015 3 27 2.5230272855168963 3 28 2.194557553361612 3 29 2.920314987375221 3 30 2.203009530898143 3 31 1.9714658290035203 3 32 2.627774617223565 3 33 2.2567761529620753 3 34 2.322087506719143 3 35 1.5073514813655535 3 36 3.0512808059310217 3 37 3.5109097435481473 3 38 3.462098246817831 3 39 3.466797975767662 3 40 7.400933253893939 3 41 8.26404788107164 3 42 4.753776170207381 3 43 -0.47290885039425135 3 44 0.9999676107397235 3 45 3.704125581970061 3 46 0.736858624349118 3 47 -4.395188538034656 3 48 0.6753137906630009 3 49 2.6733812859223995 3 50 1.4090097356139744 3 51 2.944542308264438 3 52 2.5986791287968956 3 53 2.766720612682338 3 54 3.8698261268299987 3 55 2.964547011146472 3 56 3.6412855407028957 4 1 1.4693580598734997 4 2 2.270946246958708 4 3 2.407270634997211 4 4 2.3921864843551077 4 5 1.5414595095690373 4 6 2.4771975516654123 4 7 2.48554656681722 4 8 2.388115818750336 4 9 2.509243055047044 4 10 2.2547753601399307 4 11 2.8831480250741777 4 12 4.868307267559892 4 13 4.968096348047212 4 14 4.915144551859839 4 15 4.396191490128366 4 16 5.204712989171433 4 17 5.363803821369757 4 18 5.776666984276595 4 19 4.36169938377202 4 20 5.2080254288200365 4 21 3.961395475770175 4 22 4.285654497181078 4 23 4.120689863975713 4 24 3.882491266672325 4 25 4.0049026568705415 4 26 3.0822329260358075 4 27 3.0978304351231998 4 28 3.2496756635978343 4 29 3.3455118377689175 4 30 2.9431670112130988 4 31 2.987213860499587 4 32 3.5411604439952242 4 33 2.319768278946327 4 34 2.6527961681364687 4 35 2.609713686089961 4 36 4.106398916167244 4 37 3.9833506884300363 4 38 4.280995884613105 4 39 4.986483015137736 4 40 8.456051364130154 4 41 10.65774866847321 4 42 6.344327351309744 4 43 7.550713196849841 4 44 7.299180209164916 4 45 5.987590148899201 4 46 5.965205081042033 4 47 5.880402013146448 4 48 5.431219302474023 4 49 8.36629467174917 4 50 5.857828633251771 4 51 5.377613174406168 4 52 5.83489960123784 4 53 5.601366281973679 4 54 6.37376313470401 4 55 5.0905312631149755 4 56 5.60191546196274 5 1 1.2324713245615655 5 2 2.225095247564404 5 3 2.209632839721621 5 4 2.141792783567709 5 5 1.8421863417919297 5 6 2.5752593324043644 5 7 2.1743412367142554 5 8 2.3953841409792886 5 9 2.0452818194322617 5 10 1.9495058265239393 5 11 2.308889999635049 5 12 4.526211567983879 5 13 3.8624633983197754 5 14 4.762025230236581 5 15 4.864574288432422 5 16 5.008286580934005 5 17 4.803658454925184 5 18 4.978605203537647 5 19 4.0591554709918825 5 20 4.360175640812763 5 21 3.510214373407969 5 22 3.8412571622325586 5 23 3.294463334599577 5 24 3.996603925666875 5 25 2.94675607903892 5 26 2.4907126353029128 5 27 2.961609962682253 5 28 2.0508264812840835 5 29 3.0186190455217954 5 30 3.2383214630604655 5 31 2.4577771554723284 5 32 2.187374859500977 5 33 2.6119548325502038 5 34 1.9770844782515482 5 35 2.2709493008688852 5 36 2.5362596066578575 5 37 3.3591229476668616 5 38 2.8032854061152293 5 39 4.0617707195592985 5 40 8.081975046746756 5 41 10.281915839884483 5 42 6.0975678116368215 5 43 6.564159592973404 5 44 6.56096094811101 5 45 4.494192208257161 5 46 5.332739908419381 5 47 5.166349923503805 5 48 4.827706280063364 5 49 7.491976076958135 5 50 4.878482782252377 5 51 3.564530194394056 5 52 3.929508928918036 5 53 3.860179122676282 5 54 4.354683788853013 5 55 3.7862914084814214 5 56 3.3567307254394194 6 1 1.9401636322538778 6 2 2.8097106321797867 6 3 2.7019405320293117 6 4 2.749485091260013 6 5 2.3191094187150085 6 6 2.7829516400966696 6 7 2.7281873905604073 6 8 2.5415379871331325 6 9 2.6298972040476443 6 10 2.8418135188316356 6 11 2.8473515380965893 6 12 5.218519260291572 6 13 5.170155706012082 6 14 5.4697175379288865 6 15 5.403035826893962 6 16 6.031363504010926 6 17 5.972889224155949 6 18 6.086297511229951 6 19 4.828386240222656 6 20 5.23709871773584 6 21 4.03329129648489 6 22 4.241257162232564 6 23 4.1406171807534236 6 24 4.496603925666875 6 25 4.292909925192767 6 26 3.159943404533685 6 27 3.438533039605332 6 28 3.489288019745622 6 29 3.9878498147525647 6 30 3.7229368476758538 6 31 3.7039310016261737 6 32 3.8181440902702093 6 33 3.319647140242509 6 34 3.1540075551746227 6 35 3.432487762407348 6 36 4.12087499127324 6 37 4.028353716897634 6 38 4.503285406115225 6 39 5.015616873405456 6 40 8.428128892900602 6 41 4.943454301422943 6 42 6.843721657790667 6 43 8.510313439127252 6 44 7.753268640418703 6 45 6.355730669795626 6 46 6.701970677650149 6 47 7.274042231196116 6 48 6.827706280063364 6 49 8.391976076958134 6 50 6.409252013021607 6 51 6.587607117470977 6 52 6.214124313533421 6 53 6.983256045753205 6 54 7.454683788853014 6 55 6.117060639250653 6 56 6.749038417747112 7 1 1.399858482857816 7 2 2.2041797993762273 7 3 2.2782278810439323 7 4 2.34344567612969 7 5 1.9117985477677735 7 6 2.7292962046230542 7 7 2.542681986873184 7 8 2.6121673577625018 7 9 2.2582506687647452 7 10 2.596676837331316 7 11 2.7947768400673425 7 12 4.967025299706698 7 13 5.046315909445013 7 14 4.963169540471803 7 15 5.075699526830391 7 16 5.428439155632034 7 17 5.253372377366375 7 18 5.962584860244576 7 19 4.548983824456599 7 20 4.087702659248876 7 21 3.443272224647636 7 22 3.521549597070454 7 23 3.309275794866579 7 24 2.984397949824533 7 25 3.551333319979797 7 26 2.634259997032096 7 27 2.6880562436739908 7 28 2.5364590305529973 7 29 2.6842897384652176 7 30 3.127132651871655 7 31 2.190962152293693 7 32 2.474914592495253 7 33 1.964656676161134 7 34 2.435317154666045 7 35 2.428546249374925 7 36 2.901739581228739 7 37 2.7378896355244606 7 38 2.5554150946085556 7 39 3.1470218320831407 7 40 6.573901302309373 7 41 -0.27847831141876256 7 42 3.849761072920991 7 43 5.918514329146326 7 44 5.645767051098936 7 45 4.25439564118787 7 46 4.737444294941948 7 47 5.778365180973605 7 48 5.323065466331645 7 49 7.749128015928253 7 50 4.93741475936617 7 51 5.017295610795838 7 52 4.276234930189492 7 53 5.012181665587914 7 54 5.2329418943838455 7 55 4.16575739703832 7 56 4.938676052839291 8 1 1.4980515070909064 8 2 2.320792770380571 8 3 2.1791243651453485 8 4 1.9485724445846557 8 5 1.9458369284933639 8 6 2.3734340390536417 8 7 2.5649540137677107 8 8 2.2369747537563427 8 9 2.0511488337738513 8 10 2.323039072938542 8 11 2.5523059057628146 8 12 5.015911698361975 8 13 5.020612029349756 8 14 4.726301631801121 8 15 5.1617059703098676 8 16 5.557308745210406 8 17 5.418521557923874 8 18 5.982125412142601 8 19 5.090446214147036 8 20 3.78990184681016 8 21 3.664451661543559 8 22 4.29679823133295 8 23 3.9277097492019237 8 24 4.140932478469999 8 25 4.036065075127581 8 26 2.910138971678407 8 27 3.4209319965805562 8 28 3.2458721136178568 8 29 3.27337784604331 8 30 3.298165009344693 8 31 2.0590809364371268 8 32 2.29519754528976 8 33 2.0744059407640236 8 34 2.2081144652137397 8 35 2.263256993176576 8 36 2.558554261155905 8 37 3.0295271197659517 8 38 2.9869881440552533 8 39 2.7884982293376552 8 40 5.700879870736323 8 41 -1.3628038472080881 8 42 4.414125829889755 8 43 6.387627650339766 8 44 5.61193878383461 8 45 4.084413851021175 8 46 5.3945391261508 8 47 5.155658919592465 8 48 5.519101325695697 8 49 7.764466298600897 8 50 5.772615767910786 8 51 5.878480650717396 8 52 6.454411145345677 8 53 6.517154350837952 8 54 7.640211820143758 8 55 7.367256206395375 8 56 5.522441286065234 9 1 1.1187204736425755 9 2 2.1926241788101493 9 3 2.447685937066751 9 4 2.2586069428597426 9 5 2.024283006189485 9 6 2.6881933010905428 9 7 2.1356754776945124 9 8 2.4021914929193855 9 9 2.4898019011204866 9 10 2.3715616468098517 9 11 2.579754533331439 9 12 5.241800403926696 9 13 4.874444337734339 9 14 5.389118490958161 9 15 5.362395935811591 9 16 5.9318400186467315 9 17 5.5539647857624885 9 18 6.220538491488632 9 19 5.269162278343821 9 20 5.706806001602416 9 21 4.312869240664604 9 22 4.252625439972519 9 23 4.007805744402162 9 24 5.084010324577697 9 25 4.148934431659754 9 26 3.1458521860176845 9 27 3.8881586352486224 9 28 3.5060341055182604 9 29 3.976617684051412 9 30 3.647647535218397 9 31 3.366490565955651 9 32 4.157354437445157 9 33 3.945787371692475 9 34 3.4545521433298347 9 35 3.396000356008436 9 36 4.606851846276648 9 37 4.505753308456512 9 38 4.677553615781669 9 39 5.074908908803685 9 40 8.231328348312445 9 41 4.660744975350784 9 42 6.9257502486688125 9 43 7.7794080613192165 9 44 7.808203970575271 9 45 6.3297265853844635 9 46 7.171950255594329 9 47 7.295076948691008 9 48 6.63887033724512 9 49 9.46440630160075 9 50 5.8206883642809615 9 51 6.023073421078873 9 52 5.365315600122937 9 53 5.757592328939044 9 54 6.564690596204951 9 55 6.195889774716953 9 56 6.363674224418315 10 1 1.1983664913416945 10 2 2.3076684265977576 10 3 2.1998983264472827 10 4 2.232058270293372 10 5 1.6879998203487716 10 6 2.8386357789666476 10 7 2.578153353800708 10 8 2.3313950327423925 10 9 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2.1331932579322626 115 31 1.8227344204295974 115 32 1.6548962270223413 115 33 0.7256300462254188 115 34 1.3719562731233452 115 35 1.24103477095435 115 36 2.0559177263159754 115 37 2.7001485886924996 115 38 2.402430705260528 115 39 2.8489502067387846 115 40 6.301633166404869 115 41 6.46054831851696 115 42 1.2069695210385305 115 43 -3.277720748906937 115 44 -4.245876658726594 115 45 2.1625682766332304 115 46 -1.5416190659395959 115 47 -1.050744093590211 115 48 -1.698789446432361 115 49 -11.009733324751267 115 50 -1.702713798944206 115 51 -1.4893159594520995 115 52 -1.0653628659537588 115 53 -1.9945217320245732 115 54 -4.475230741061516 115 55 -1.2931957710057596 115 56 0.5661324348411299 116 1 1.4597179097520794 116 2 2.3463749024889182 116 3 2.210567419160874 116 4 2.2053441854368643 116 5 2.030899497794806 116 6 2.667351352533764 116 7 2.180667620610734 116 8 2.3292446729706597 116 9 2.3637577360390196 116 10 2.44698965111057 116 11 -1.228780740839433 116 12 -12.223752486653073 116 13 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1086.0 5 280.0 6 112.0 7 148.0 8 271.0 9 266.0 10 195.0 11 163.0 12 164.0 13 152.0 14 179.0 15 265.0 16 291.0 17 335.0 18 437.0 19 479.0 20 662.0 21 800.0 22 980.0 23 1242.0 24 1431.0 25 1604.0 26 1761.0 27 1989.0 28 2253.0 29 2720.0 30 3339.0 31 4059.0 32 4567.0 33 5290.0 34 6239.0 35 7410.0 36 9576.0 37 12355.0 38 12676.0 39 8114.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.67625822666184 0.26197525079338035 0.043662541798896726 0.01810398074588401 8 99.19597026687399 0.4430150582522204 0.0713509829396605 0.28966369193414415 9 96.41647675235883 2.0755681455134076 0.6166002854039317 0.8913548167238183 10 36.555131946071434 42.334561564183936 13.051905177738494 8.058401312006135 11 28.410470490511386 36.985367723797154 18.45541096036293 16.148750825328534 12 32.326255031841704 15.59711188260101 23.64699367425614 28.429639411301146 13 23.058978920122282 16.07247472864584 27.25471607673544 33.613830274496436 14 25.09451443541602 20.223426800566553 30.180722250029284 24.501336513988136 15 29.236600469784324 21.081571642109758 24.920990817851806 24.76083707025411 16 31.1927289775391 19.629842731108553 24.027191748710734 25.150236542641608 17 29.209797657082003 20.806176783812568 26.56230031948882 23.421725239616613 18 26.303514376996805 22.616613418530353 27.1395101171459 23.940362087326942 19 30.811052833567864 24.684448424374228 21.25922135516609 23.245277386891814 20 24.184246714067946 29.088893389760045 23.29947020008741 23.427389696084596 21 24.80032374177334 24.104917893122618 27.63199931843837 23.462759046665674 22 23.556473770526722 22.257246916998575 22.875977082490255 31.310302229984448 23 26.997295052288557 22.927094204596283 23.39140806372601 26.684202679389152 24 23.195459095652915 23.590551851930737 29.148473940917125 24.065515111499224 25 28.97870074547391 24.70713525026624 24.550585729499467 21.763578274760384 26 22.39913943679973 21.769692838580497 27.54121756912197 28.289950155497806 27 21.707426280889532 21.17865290718748 30.024946163194816 27.08897464872817 28 23.4518275259095 25.602422484752847 30.156309975689854 20.789440013647802 29 25.192295399825284 20.915986619223148 34.248822789934586 19.642895191016983 30 19.60141029601304 19.86237896912048 41.84765820560071 18.688552529265774 31 18.989283507680508 25.048469224705457 35.79904979014424 20.1631974774698 32 25.422102839600917 18.67805065234075 39.14364287541571 16.75620363264262 33 21.813061381237866 19.44912632544644 38.05018028205073 20.687632011264963 34 17.119689720940638 26.6880480762059 41.23344450245602 14.95881770039744 35 19.86682292776476 19.829533347538888 46.49584487534626 13.807798849350094 36 17.09419008458146 25.162451797089712 42.94479834672007 14.798559771608751 37 17.20682553064339 20.129766186090688 45.109757008547916 17.553651274718003 38 25.02322998216365 20.898439586025695 40.127524591739736 13.950805840070919 39 20.306681036782518 19.952407883644746 39.85679681580997 19.884114263762765 40 13.671711591886645 17.51815422239861 39.691512297675615 29.11862188803913 41 14.239204014312271 14.994573801669764 37.324723048986215 33.44149913503175 42 19.95243579899326 22.376503711287434 40.74844296561727 16.92261752410204 43 13.860540586703104 16.974465177847048 45.301891626457156 23.863102608992698 44 14.550748841142905 19.840275770519533 39.76084187087174 25.848133517465822 45 15.980960309074804 20.071719015144236 45.38362202371423 18.563698652066723 46 18.21771190148435 20.267631334635208 41.060281076928 20.45437568695244 47 14.8968808590421 23.83245270155105 41.77283961138571 19.497826828021136 48 16.299014378973418 27.26671502325383 41.1304774501856 15.30379314758715 49 15.509526867908372 20.75251552130165 35.84457289659602 27.893384714193964 50 22.38170902072025 20.522965032578675 41.40530856429889 15.690017382402182 51 18.426027792315992 25.92845775076637 40.48791429456437 15.157600162353269 52 15.557854103797226 24.17385555401529 44.29136587082396 15.976924471363525 53 16.17855733017529 21.465074843431594 46.87023226536077 15.486135561032338 54 17.068796173719946 24.536661399837726 38.200879702780036 20.1936627236623 55 15.713553567233978 28.84537359328621 39.26199577185078 16.179077067629034 56 22.747014694426362 23.071423235761774 38.49683594958862 15.684726120223244 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 2.0 5 2.0 6 20.5 7 39.0 8 129.5 9 220.0 10 310.0 11 400.0 12 433.5 13 626.0 14 785.0 15 949.5 16 1114.0 17 1248.0 18 1382.0 19 1583.0 20 1784.0 21 2252.5 22 3077.0 23 3433.0 24 3826.5 25 4220.0 26 4448.0 27 4676.0 28 4587.0 29 4009.5 30 3521.0 31 2975.0 32 2429.0 33 2083.5 34 1738.0 35 1597.0 36 1456.0 37 1496.0 38 1603.0 39 1670.0 40 1838.5 41 2007.0 42 3082.0 43 4157.0 44 4013.0 45 3869.0 46 3457.5 47 3034.5 48 3023.0 49 3331.0 50 3639.0 51 3870.0 52 4101.0 53 4537.5 54 4840.5 55 4707.0 56 4614.0 57 4521.0 58 4340.5 59 4160.0 60 3782.0 61 3404.0 62 3165.0 63 2510.5 64 2095.0 65 1848.0 66 1601.0 67 1410.5 68 1220.0 69 1081.5 70 943.0 71 816.5 72 538.0 73 386.0 74 276.0 75 166.0 76 135.5 77 105.0 78 75.5 79 31.0 80 16.0 81 10.0 82 4.0 83 3.0 84 2.0 85 1.0 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.022363740921386127 14 0.0010649400438755298 15 0.2577154906178782 16 0.05324700219377649 17 0.0021298800877510597 18 0.0021298800877510597 19 0.10436412429980192 20 0.09903942408042428 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0021298800877510597 26 0.0106494004387553 27 0.10649400438755298 28 0.12140316500181039 29 0.038337841579519076 30 0.022363740921386127 31 0.029818321228514834 32 0.09158484377329557 33 0.1703904070200848 34 0.05431194223765202 35 0.044727481842772254 36 0.029818321228514834 37 0.20766330855572832 38 0.2907286319780196 39 0.20127366829247512 40 0.7167046495282315 41 0.8892249366360674 42 0.142701965879321 43 0.24067644991586976 44 0.5228855615428851 45 0.21618282890673254 46 0.20340354838022617 47 0.0319482013162659 48 0.1640007667568316 49 0.5133011011480054 50 0.13737726565994335 51 0.29711827224127285 52 0.13098762539669018 53 0.18423462759046666 54 0.24919597026687398 55 0.25984537070562924 56 0.20553342846797726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 93902.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.6821792933058 #Duplication Level Percentage of deduplicated Percentage of total 1 86.29793050170106 61.86023726864177 2 7.916982365438042 11.350130987625397 3 2.5939296697419443 5.578155949820025 4 1.2003981518622513 3.4418862218057122 5 0.5719718916670381 2.050009584460395 6 0.37735288437253944 1.6229686268663075 7 0.20947542006507108 1.051095823305148 8 0.16787746430746833 0.962705799663479 9 0.10845181322517866 0.6996656088262231 >10 0.5229457295241491 6.32254904048902 >50 0.0178276953246869 0.842367574705544 >100 0.013370771493515177 2.4397776405188387 >500 0.0 0.0 >1k 0.0014856412770572417 1.7784498732721348 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1670 1.7784498732721348 No Hit ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATGAG 423 0.45046963855934913 No Hit ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 403 0.42917083768183856 No Hit ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 382 0.40680709676045235 No Hit ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 381 0.4057421567165769 No Hit ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGACACACGCAGGGATGAG 171 0.1821047475027156 No Hit ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG 150 0.15974100658132948 No Hit ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCGACACGCAGGGATGAG 145 0.1544163063619518 No Hit ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 123 0.13098762539669018 No Hit ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 113 0.12033822495793488 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0031948201316265895 0.0 0.0 0.0 0.0 10 0.011714340482630828 0.0 0.0 0.0 0.0 11 0.013844220570381887 0.0 0.0 0.0 0.0 12 0.018103980745884006 0.0 0.0 0.0 0.0 13 0.019168920789759538 0.0 0.0 0.0 0.0 14 0.022363740921386127 0.0 0.0 0.0 0.0 15 0.025558561053012716 0.0 0.0 0.0 0.0 16 0.029818321228514834 0.0 0.0 0.0 0.0 17 0.034078081404016955 0.0 0.0 0.0 0.0 18 0.04579242188664778 0.0 0.0 0.0 0.0 19 0.04898724201827437 0.0 0.0 0.0 0.0 20 0.05857170241315414 0.0 0.0 0.0 0.0 21 0.06496134267640732 0.0 0.0 0.0 0.0 22 0.0713509829396605 0.0 0.0 0.0 0.0 23 0.07987050329066474 0.0 0.0 0.0 0.0 24 0.09051990372942004 0.0 0.0 0.0 0.0 25 0.09690954399267321 0.0 0.0 0.0 0.0 26 0.11181870460693064 0.0 0.0 0.0 0.0 27 0.12033822495793486 0.0 0.0 0.0 0.0 28 0.1256629251773125 0.0 0.0 0.0 0.0 29 0.13098762539669018 0.0 0.0 0.0 0.0 30 0.13418244552831676 0.0 0.0 0.0 0.0 31 0.1395071457476944 0.0 0.0 0.0 0.0 32 0.142701965879321 0.0 0.0 0.0 0.0 33 0.14376690592319652 0.0 0.0 0.0 0.0 34 0.14376690592319652 0.0 0.0 0.0 0.0 35 0.14802666609869863 0.0 0.0 0.0 0.0 36 0.15122148623032522 0.0 0.0 0.0 0.0 37 0.1533513663180763 0.0 0.0 0.0 0.0 38 0.15441630636195183 0.0 0.0 0.0 0.0 39 0.16187088666908053 0.0 0.0 0.0 0.0 40 0.16506570680070712 0.0 0.0 0.0 0.0 41 0.17039040702008476 0.0 0.0 0.0 0.0 42 0.17252028710783582 0.0 0.0 0.0 0.0 43 0.17571510723946243 0.0 0.0 0.0 0.0 44 0.18103980745884007 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCCC 15 0.008165706 50.369408 38 GCGACAC 45 1.2551027E-10 50.315224 39 CGTCTCA 55 0.0 50.315224 41 CCACACG 45 1.2551027E-10 50.315224 40 ACGCCCA 15 0.0082005495 50.31522 39 CTAGTCT 15 0.0082005495 50.31522 39 TCGTCTC 40 2.4938345E-9 50.31522 40 AGTCTCA 20 4.0367898E-4 50.31522 41 TGATACA 20 4.058286E-4 50.26115 34 CAAGAGA 15 0.008235503 50.261147 36 ATACAAA 15 0.008235503 50.261147 36 CAACGAT 85 0.0 50.18026 46 CGATTAA 75 0.0 50.18026 49 CCTAGTT 15 0.008288142 50.180256 48 TCCTAGT 15 0.008288142 50.180256 47 CTAGTTT 15 0.008288142 50.180256 49 CGGATGA 15 0.008288142 50.180256 49 CACTAAA 15 0.008288142 50.180256 47 CTCAACG 85 0.0 50.126476 44 ATTCAAG 15 0.008323373 50.126472 33 >>END_MODULE