FastQCFastQC Report
Fri 17 Jun 2016
SRR1526234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28169
Sequences flagged as poor quality0
Sequence length56
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1450.5147502573751287No Hit
CATGATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.23785011892505947No Hit
CATGATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG420.14910007455003726No Hit
CATGATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.1420000710000355No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG400.1420000710000355No Hit
CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT390.1384500692250346No Hit
CATGATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA390.1384500692250346No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG380.13490006745003372No Hit
CATGATGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.13135006567503285No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG340.12070006035003018No Hit
CATGATGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.11005005502502752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTAGC150.00812185550.34470441
CCGTTGT150.00812185550.34470438
CGTTGTA150.00812185550.34470439
GTTGTAG150.00812185550.34470440
TATGCTT204.0475128E-450.1645835
ATGCTTG204.0475128E-450.1645836
ATATGCT204.0475128E-450.1645834
TAGCGCC150.00823804750.16457744
ACCCGTT150.00823804750.16457736
GCTACCC150.00823804750.16457733
CCCGTTG150.00823804750.16457737
TGTAGCG150.00823804750.16457742
TACCCGT150.00823804750.16457735
AGCGCCG150.00823804750.16457745
AGATTAA251.9959843E-550.16457749
CATATGC251.9959843E-550.16457733
CTACCCG150.00823804750.16457734
TGCTACC150.00829660550.07499732
GCATATG252.0172936E-550.07499732
CCAGTAG301.0134299E-649.9857426