##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526234_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28169 Sequences flagged as poor quality 0 Sequence length 56 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.258475629237815 39.0 38.0 40.0 35.0 40.0 2 37.342433171216584 39.0 38.0 40.0 33.0 40.0 3 37.83478291739146 39.0 38.0 40.0 35.0 40.0 4 37.448684724342364 39.0 38.0 40.0 33.0 40.0 5 36.525684262842134 39.0 37.0 39.0 31.0 40.0 6 37.56143278071639 39.0 38.0 40.0 33.0 40.0 7 37.9993609996805 39.0 38.0 40.0 35.0 40.0 8 37.848840924420465 39.0 38.0 40.0 35.0 40.0 9 37.80538890269445 39.0 38.0 40.0 35.0 40.0 10 37.28439064219532 39.0 38.0 40.0 33.0 40.0 11 36.44634172317086 38.0 35.0 39.0 31.0 40.0 12 34.4046292023146 38.0 33.0 39.0 22.0 40.0 13 34.050978025489016 38.0 33.0 39.0 21.0 40.0 14 34.19233909616955 38.0 33.0 39.0 22.0 40.0 15 34.13724306862154 38.0 33.0 39.0 21.0 40.0 16 33.519187759593876 38.0 31.0 39.0 21.0 40.0 17 33.54077177038589 38.0 31.0 39.0 21.0 40.0 18 32.950370975185486 37.0 30.0 39.0 18.0 40.0 19 33.88544144272072 37.0 31.0 39.0 23.0 40.0 20 33.97582448791224 37.0 31.0 39.0 24.0 40.0 21 34.86076893038447 38.0 33.0 39.0 25.0 40.0 22 34.57570378785189 38.0 33.0 39.0 24.0 40.0 23 35.033370016685005 38.0 33.0 39.0 26.0 40.0 24 34.66250133125067 38.0 33.0 39.0 24.0 40.0 25 34.95402747701374 38.0 33.0 39.0 26.0 40.0 26 35.73666086833043 38.0 35.0 39.0 29.0 40.0 27 35.71074585537293 38.0 35.0 39.0 29.0 40.0 28 35.695587347793676 38.0 35.0 39.0 29.0 40.0 29 35.55525577762789 38.0 35.0 39.0 28.0 40.0 30 35.47374773687387 38.0 35.0 39.0 28.0 40.0 31 35.94848947424474 38.0 35.0 39.0 30.0 40.0 32 35.66207533103766 38.0 35.0 39.0 29.0 40.0 33 35.84493592246796 38.0 35.0 39.0 30.0 40.0 34 35.83801341900671 38.0 35.0 39.0 30.0 40.0 35 35.710461855230925 38.0 35.0 39.0 30.0 40.0 36 34.827043913521955 38.0 34.0 39.0 29.0 40.0 37 34.64081082040541 38.0 34.0 39.0 28.0 40.0 38 34.32113316056658 38.0 33.0 39.0 27.0 40.0 39 33.986935993467995 37.0 33.0 39.0 26.0 40.0 40 30.39039369519685 35.0 26.0 39.0 10.0 40.0 41 26.75384287692144 32.0 15.0 38.0 6.0 39.0 42 31.71518335759168 35.0 30.0 38.0 17.0 40.0 43 30.00699350349675 35.0 28.0 38.0 7.0 39.0 44 29.905285952642977 35.0 27.0 38.0 6.0 39.0 45 31.311690155845078 35.0 30.0 38.0 11.0 40.0 46 31.009123504561753 35.0 29.0 39.0 4.0 40.0 47 30.48141574070787 35.0 28.0 39.0 4.0 40.0 48 30.749298874649437 35.0 29.0 39.0 4.0 40.0 49 27.71184635592318 35.0 17.0 38.0 4.0 40.0 50 30.343072171536086 35.0 28.0 38.0 4.0 40.0 51 30.6046718023359 35.0 29.0 39.0 4.0 40.0 52 30.460932230466117 35.0 29.0 39.0 4.0 40.0 53 30.052682026341014 35.0 28.0 39.0 4.0 40.0 54 28.901416450708226 35.0 23.0 39.0 4.0 40.0 55 29.35528417764209 35.0 27.0 39.0 4.0 40.0 56 28.055877027938514 35.0 17.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.13905977000624148 1 2 1.2785245403069254 1 3 0.5724039499192557 1 4 0.8398161071678771 1 5 1.6089485223639954 1 6 0.571099703449832 1 7 0.27325302603118473 1 8 0.1238023472591081 1 9 0.35631927684135434 1 10 -0.5061689190754066 1 11 -3.4872526139872733 1 12 -14.01503887441163 1 13 -9.409454007908494 1 14 -5.889880435063052 1 15 -7.9431812994958335 1 16 -23.509502778066718 1 17 -4.6058093099028135 1 18 -12.632460870816452 1 19 -6.196913137396958 1 20 -5.930670408836061 1 21 -6.687461594991731 1 22 -3.654610520952982 1 23 -4.080062139853933 1 24 -4.706131551287495 1 25 -3.793631124961042 1 26 1.1458264918201166 1 27 1.0652040112530088 1 28 0.9780371253538576 1 29 1.610381467941167 1 30 1.861904139774012 1 31 0.20432440623936543 1 32 0.4615719863316414 1 33 0.5862306576277234 1 34 0.03896547757938151 1 35 0.06636752565837156 1 36 1.9101818791625789 1 37 1.9458952645531937 1 38 0.9067893283720423 1 39 1.9689368492073456 1 40 5.8178602720584 1 41 -8.99303943535725 1 42 -10.59854094249695 1 43 -17.00434001388362 1 44 -22.39264138826472 1 45 -12.846328890525598 1 46 -18.688505451330833 1 47 -23.951102202809032 1 48 -16.718443705379535 1 49 -20.37261548735782 1 50 -15.875244121045327 1 51 -13.417134600261523 1 52 -13.120629096966045 1 53 -13.380449250905206 1 54 -12.718259433252731 1 55 -12.776758060688962 1 56 -8.145349827127657 2 1 0.9116372255105247 2 2 2.2646814289028327 2 3 1.3502549716727188 2 4 1.5576803128369576 2 5 2.972824593557135 2 6 1.166353493825575 2 7 1.275889809155771 2 8 1.448126671583431 2 9 1.3965302194913178 2 10 1.0936992417683626 2 11 1.153485685287606 2 12 -1.897042829586315 2 13 -2.408794812127347 2 14 2.328313368496609 2 15 3.2638061757843246 2 16 2.810866371570725 2 17 3.318383175265282 2 18 -1.52303437114605 2 19 3.0166662956946695 2 20 2.0752623531942618 2 21 2.8042984577439327 2 22 2.3401159127978417 2 23 2.771618809387995 2 24 2.1046792595233157 2 25 2.4271994617232053 2 26 1.7430578695392995 2 27 1.4943404647797038 2 28 0.6965605268040846 2 29 1.9538224699187552 2 30 1.5520821226349213 2 31 2.057323747043583 2 32 2.1682298637212227 2 33 2.0338245930265373 2 34 1.427890988456113 2 35 1.4256292263834922 2 36 3.2463717275475474 2 37 3.4607271696289956 2 38 4.049834812880945 2 39 4.457400923037277 2 40 6.911466072981273 2 41 -3.3991040365438003 2 42 -4.562285174533866 2 43 -9.716930653303528 2 44 -14.534368481211326 2 45 -6.1291238806376604 2 46 -10.472289235114616 2 47 -19.668966408478116 2 48 -8.557599934779667 2 49 -13.433261499223343 2 50 -8.458632387360424 2 51 -3.234537368883803 2 52 -3.1001940277504865 2 53 -3.0607392970489116 2 54 -1.9819377457115337 2 55 -2.744457467412758 2 56 1.4479263759046432 3 1 0.9079786889251622 3 2 1.4366326484150278 3 3 0.9203769228922312 3 4 1.222924215275981 3 5 2.1136782520937203 3 6 1.5974510548011835 3 7 0.9002800530582107 3 8 0.5481266715834323 3 9 1.1434814390035157 3 10 1.0924797295732418 3 11 1.7553149535802888 3 12 3.119420585047827 3 13 2.5973027488482643 3 14 2.6216060514234343 3 15 2.9784403221257847 3 16 4.424281005717063 3 17 4.1171636630701585 3 18 5.189160750805172 3 19 3.442276051792234 3 20 2.9301404019747466 3 21 3.2030789455488105 3 22 3.4163354249929654 3 23 3.0746675898757942 3 24 2.9796792595233157 3 25 3.419882388552473 3 26 2.117448113441739 3 27 2.363852659901653 3 28 1.4679019902187207 3 29 1.6556517382114393 3 30 1.7362284640983319 3 31 1.347567649482606 3 32 2.0298152295748864 3 33 1.3490684954655592 3 34 1.0754519640658629 3 35 1.348799958090808 3 36 2.956127825108524 3 37 2.5729222915802197 3 38 2.5230055445882584 3 39 2.9006936059641006 3 40 7.65231973151786 3 41 9.269798402480586 3 42 4.473080679124674 3 43 0.6666059320623283 3 44 3.7046559090325744 3 45 4.68002246082575 3 46 3.1527107648853843 3 47 -1.2842103109171426 3 48 3.3302049432691128 3 49 5.855762891020561 3 50 2.270026149224943 3 51 4.054487021360099 3 52 4.313830362493416 3 53 3.7465777761218213 3 54 2.6540378640445645 3 55 2.3043230203921183 3 56 2.685731253953424 4 1 1.081234502878651 4 2 2.177330322833633 4 3 1.382586225217814 4 4 1.5293195641131945 4 5 2.385189880000695 4 6 1.01547431061514 4 7 0.6491172623605337 4 8 1.0760336483276163 4 9 0.23941167156165477 4 10 1.7936425202709145 4 11 2.6384544884640135 4 12 4.746746166443181 4 13 4.835674841871523 4 14 4.457071167702509 4 15 4.6784403221257875 4 16 5.530094959205435 4 17 5.460186918884112 4 18 5.872881681037725 4 19 4.224834191327112 4 20 3.7638613322073056 4 21 2.7600556897348554 4 22 3.547149378481336 4 23 3.068272241038585 4 24 3.2442141432442426 4 25 3.394300993203636 4 26 2.1627969506510425 4 27 2.3417596366458397 4 28 2.0987159437070915 4 29 2.6015819707695798 4 30 2.1629726501448445 4 31 1.9126839285523758 4 32 2.774582671435347 4 33 1.6101150070934693 4 34 1.0603356849960974 4 35 1.824381353439641 4 36 3.3677557320852642 4 37 2.48455019855696 4 38 4.076493916681287 4 39 4.539647094336196 4 40 8.440110429192277 4 41 11.216891425736403 4 42 7.1858713767990885 4 43 8.03462918787628 4 44 7.393028002055836 4 45 6.900371298035058 4 46 5.016082857908643 4 47 3.8710222472223954 4 48 4.9767165711760875 4 49 6.470879170090328 4 50 3.092700567829592 4 51 3.1347195794996345 4 52 2.1062722229585304 4 53 2.4837870784474028 4 54 3.3697355384631713 4 55 3.374090462252582 4 56 4.470614974883656 5 1 0.6433445425836979 5 2 0.9476082581711296 5 3 0.13074277655076827 5 4 0.2162168982028092 5 5 1.3386782520937217 5 6 0.5565973962645998 5 7 0.12954834574113505 5 8 0.3749559398761164 5 9 -0.18883563416721927 5 10 0.5571138759147019 5 11 1.836412514555903 5 12 4.493201072852706 5 13 3.9326686025068014 5 14 4.499045075813683 5 15 4.410147639198961 5 16 5.274281005717064 5 17 5.147651467948208 5 18 5.403794897146632 5 19 4.114227271304429 5 20 4.538676987340601 5 21 2.999420408963445 5 22 3.7791403030417428 5 23 3.5521066142660445 5 24 3.7632158448891673 5 25 3.256467754406131 5 26 2.401594454905151 5 27 2.5919014403894565 5 28 2.428267843877258 5 29 2.1733346650407057 5 30 2.917935781171508 5 31 1.4719578933850457 5 32 0.9731079125017104 5 33 0.4972392271728765 5 34 1.4522812323585512 5 35 0.693921909310319 5 36 1.4414936787670598 5 37 1.509507657433879 5 38 2.12300554458826 5 39 2.359839947427517 5 40 7.545612414444687 5 41 8.991139865895224 5 42 4.36454409375882 5 43 6.014776663769641 5 44 6.807094933422825 5 45 4.919656607167216 5 46 4.552101008787822 5 47 4.916399445180421 5 48 4.881424455464238 5 49 8.152104354435195 5 50 3.395026149224943 5 51 3.0825358018479 5 52 2.9241962161519517 5 53 3.8416997273413322 5 54 4.481476888434813 5 55 3.548225459416507 5 56 3.886950766148548 6 1 1.134901765848241 6 2 2.104581366363746 6 3 1.1094794869947933 6 4 0.7940780614298291 6 5 2.595088508503977 6 6 1.281425413775544 6 7 0.8900236428018005 6 8 1.4224856459424018 6 9 1.5441224646445448 6 10 1.3988899859834945 6 11 2.5162123894777295 6 12 4.931600072227319 6 13 3.4755078770533956 6 14 5.211990666808049 6 15 4.729722373407839 6 16 4.966588698024758 6 17 4.931266227172721 6 18 4.373776135420556 6 19 3.761506821022998 6 20 4.085268607102954 6 21 2.1094892019590645 6 22 2.214412348069885 6 23 2.4701804103886147 6 24 2.4380125928566514 6 25 1.8480875167576016 6 26 2.023858369851993 6 27 1.6882116342606253 6 28 2.381363528680261 6 29 3.137061994621696 6 30 2.4939207717906413 6 31 2.4475676494826075 6 32 2.6022511270107813 6 33 1.6548377262347955 6 34 2.063913502527406 6 35 2.4475179068087556 6 36 3.1638201328008293 6 37 3.0754863941443205 6 38 3.276851698434413 6 39 3.366719246989746 6 40 7.867063321261451 6 41 1.5114650691472562 6 42 3.869234525278518 6 43 6.2492982397546335 6 44 5.802091806468475 6 45 3.6845096403129354 6 46 4.425146662321282 6 47 4.99207174036491 6 48 4.340461353525523 6 49 6.774993660251326 6 50 3.9591287133275053 6 51 3.819871636744711 6 52 3.646522670185721 6 53 2.518372647916692 6 54 2.938012223018923 6 55 3.3556050716741694 6 56 4.961372279594453 7 1 1.224645355591825 7 2 2.247743759526145 7 3 1.5175991451144526 7 4 1.3923686597204252 7 5 2.3386782520937217 7 6 1.8946732770234078 7 7 1.4669467197248736 7 8 1.1147933382500952 7 9 1.4629258834479586 7 10 1.0869241740176818 7 11 2.6358705091358487 7 12 3.361087251714494 7 13 4.597302748848264 7 14 4.95771716253455 7 15 5.039551433236902 7 16 5.8298365612726215 7 17 5.533830329736823 7 18 5.933605195249612 7 19 5.131164940681117 7 20 3.5745848464191923 7 21 3.603078945548809 7 22 3.263557647215187 7 23 3.1218898120980185 7 24 3.71579037063443 7 25 3.358771277441356 7 26 1.184114780108402 7 27 1.9360748821238758 7 28 2.0929019902187207 7 29 1.8806517382114407 7 30 1.4084506863205561 7 31 0.19756764948260752 7 32 2.0937041184637692 7 33 0.8962907176877835 7 34 0.7476741862880871 7 35 0.8876888469796924 7 36 2.5505722695529656 7 37 3.1951445138024397 7 38 2.039672211254924 7 39 3.5868047170752178 7 40 6.749541953740081 7 41 2.4003539580361455 7 42 4.356414012458007 7 43 7.302717043173441 7 44 7.09910035347702 7 45 5.866133571936867 7 46 5.083266320440941 7 47 5.051900800193973 7 48 5.3468716099357785 7 49 8.144651779909445 7 50 6.145026149224943 7 51 4.371153688026762 7 52 3.699941473604529 7 53 3.952133331677377 7 54 4.329037864044565 7 55 2.665434131503229 7 56 6.01906458728676 8 1 0.8579786889251579 8 2 1.7294897912721723 8 3 0.9739483514636618 8 4 0.9479242152759824 8 5 1.5958211092365815 8 6 0.15102248337261415 8 7 0.7288514816296399 8 8 0.5338409572977199 8 9 0.6899100104320866 8 10 0.7424797295732404 8 11 1.4517435250088653 8 12 3.5872777279049757 8 13 3.3473027488482643 8 14 3.282320337137719 8 15 2.9355831792686473 8 16 4.017138148574205 8 17 4.195735091641588 8 18 4.560589322233739 8 19 3.277990337506516 8 20 3.7944261162604604 8 21 3.1673646598345258 8 22 1.9484782821358237 8 23 2.563953304161508 8 24 2.3046792595233185 8 25 2.5198823885524675 8 26 1.2531623991560252 8 27 0.9995669456159391 8 28 0.2714734187901513 8 29 -0.6336339760742717 8 30 -0.21377153590166387 8 31 -0.3238609219459647 8 32 -0.12375619899654566 8 33 -2.2616457902487213 8 34 -2.1031194645055606 8 35 -1.6440571847663392 8 36 -1.2295864606057663 8 37 -1.1985062798483526 8 38 -1.5055658839831665 8 39 -1.1314492511787506 8 40 2.64874830294643 8 41 -4.7837730260908415 8 42 -0.3769193208753272 8 43 1.4273202177766109 8 44 1.704655909032578 8 45 -0.46640611060281856 8 46 1.213425050599671 8 47 0.1979325462257151 8 48 1.3873478004119697 8 49 3.920048605306274 8 50 0.8235975777963702 8 51 0.11877273564581259 8 52 0.9102589339219875 8 53 0.27514920469324977 8 54 0.3504664354731375 8 55 0.4471801632492607 8 56 1.4785883968105686 9 1 0.6008358317823053 9 2 2.129489791272171 9 3 1.0025197800350867 9 4 1.0050670724188393 9 5 1.6815353949508633 9 6 1.1510224833726141 9 7 0.8717086244867787 9 8 0.8195552430120046 9 9 0.8899100104320894 9 10 1.5710511581446696 9 11 2.2803149535802945 9 12 3.9872777279049743 9 13 3.690159891705406 9 14 4.7680346228520065 9 15 4.535583179268642 9 16 5.359995291431353 9 17 4.7100208059273 9 18 4.6748750365194525 9 19 4.620847480363658 9 20 3.422997544831894 9 21 3.2816503741202396 9 22 3.548478282135825 9 23 3.3639533041615124 9 24 4.018964973809027 9 25 3.091310959981044 9 26 1.7388766848703057 9 27 2.085281231330228 9 28 1.6429019902187179 9 29 1.8520803096400087 9 30 2.3005141783840486 9 31 1.047567649482609 9 32 0.33338665814630986 9 33 0.7097827811798467 9 34 1.5825948212087226 9 35 0.6130856723765206 9 36 1.8561278251085227 9 37 2.144350863008789 9 38 2.037291258873971 9 39 1.7256936059641035 9 40 7.620176874375002 9 41 4.244798402480587 9 42 4.537366393410384 9 43 6.684463074919467 9 44 6.41894162331829 9 45 5.447879603682896 9 46 5.013425050599672 9 47 5.255075403368576 9 48 5.301633514697688 9 49 8.405762891020558 9 50 6.166454720653515 9 51 5.290201307074387 9 52 5.738830362493413 9 53 5.0465777761218185 9 54 6.2647521497588485 9 55 5.590037306106403 9 56 5.021445539667713 10 1 1.0276756586221296 10 2 2.1745114362938196 10 3 1.3938617713770824 10 4 1.88731815466992 10 5 2.581102494517964 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1.6862284640983347 114 31 1.7332819351968922 114 32 1.5619580867177376 114 33 1.3883542097512773 114 34 2.3111662497801504 114 35 1.1773713866622373 114 36 2.6061278251085227 114 37 1.8657794344373642 114 38 1.730148401731114 114 39 3.261407891678388 114 40 6.920176874375006 114 41 6.059084116766304 114 42 1.808794964838956 114 43 -2.094108353651958 114 44 -9.70248694811028 114 45 0.505022460825753 114 46 -5.900860663686043 114 47 -5.023496025202856 114 48 -5.1769379138737435 114 49 -18.137094251836583 114 50 -7.17640242220363 114 51 -7.438370121497044 114 52 -6.054026780363728 114 53 -7.924850795306753 114 54 -14.099533564526864 114 55 -8.588534122465024 114 56 -2.9214116031894335 115 1 0.17336330430977398 115 2 1.3866326484150306 115 3 0.5453769228922312 115 4 1.0633088306605956 115 5 1.7617551751706415 115 6 0.9609125932627265 115 7 0.22335697613512906 115 8 0.5635112869680441 115 9 -0.01998009945802437 115 10 0.06555665265015875 115 11 -1.4581465848812485 115 12 -10.05557941495217 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116 34 1.4540233926372963 116 35 1.570228529519376 116 36 2.927556396537092 116 37 1.508636577294503 116 38 1.9087198303025446 116 39 1.7256936059641035 116 40 6.241605445803575 116 41 3.7376555453377307 116 42 -2.8340621780181863 116 43 -7.344108353651958 116 44 -9.238201233824565 116 45 -1.6378346820313894 116 46 -3.7222892351146157 116 47 -2.166353168059999 116 48 -3.069795056730886 116 49 -6.244237108979441 116 50 -2.4621167079179145 116 51 -0.616941550068475 116 52 -2.0897410660780125 116 53 -3.139136509592465 116 54 -8.242390707384008 116 55 -0.6242484081793087 116 56 -1.242840174618003 117 1 0.14131202225849648 117 2 1.2199659817483663 117 3 0.6287102562255669 117 4 1.031257548609318 117 5 2.130344918760386 117 6 0.1724510548011864 117 7 0.38361338639154496 117 8 -0.01020666174990481 117 9 0.31014810567018003 117 10 0.9341463962399033 117 11 -2.7946850464197084 117 12 -8.388912748285502 117 13 -8.444363917818404 117 14 -13.236727281909896 117 15 -11.571559677874212 117 16 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-6.295962135955435 117 55 -6.695676979607882 117 56 -2.272602079379908 118 1 -0.4220213110748361 118 2 1.2666326484150332 118 3 0.18537692289223173 118 4 0.5879242152759829 118 5 1.4986782520937183 118 6 0.7624510548011898 118 7 -0.259719946941793 118 8 -0.5518733284165691 118 9 -0.0015185609964873947 118 10 0.22247972957323725 118 11 -2.599685046419708 118 12 -10.835579414952171 118 13 -10.092697251151737 118 14 -12.083393948576564 118 15 -7.3215596778742125 118 16 -12.805718994282934 118 17 -11.35283633692984 118 18 -12.970839249194832 118 19 -4.807723948207769 118 20 -5.039859598025252 118 21 -0.5469210544511895 118 22 1.931335424992966 118 23 0.5296675898757996 118 24 0.9846792595233183 118 25 1.7198823885524703 118 26 1.5274481134417357 118 27 2.1938526599016512 118 28 2.0829019902187227 118 29 0.40065173821144384 118 30 2.266228464098333 118 31 1.1675676494826064 118 32 -4.455184770425117 118 33 1.5040684954655603 118 34 1.0454519640658688 118 35 0.7387999580908087 118 36 2.416127825108525 118 37 3.4929222915802214 118 38 3.3230055445882627 118 39 4.045693605964104 118 40 8.477319731517863 118 41 4.44479840248059 118 42 -0.9369193208753295 118 43 -11.818394067937675 118 44 -9.055344090967424 118 45 2.225022460825752 118 46 -8.432289235114617 118 47 -7.419210310917141 118 48 -7.429795056730885 118 49 -13.03423710897944 118 50 -6.844973850775055 118 51 -3.3155129786399016 118 52 -3.461169637506586 118 53 -3.5134222238781803 118 54 -12.980962135955435 118 55 -2.5356769796078815 118 56 -0.6242687460465746 119 1 -0.49757686663039635 119 2 0.4977437595261449 119 3 -0.04721566970036406 119 4 0.31829458564635615 119 5 1.0794189928344622 119 6 -0.003474871124737433 119 7 -0.32934957657141695 119 8 -0.5518733284165691 119 9 -0.5185555980335224 119 10 -0.11677952968602057 119 11 -4.919685046419708 119 12 -9.44446830384106 119 13 -12.171215769670255 119 14 -9.440430985613602 119 15 -8.840078196392732 119 16 -14.392385660949603 119 17 -18.114317818411322 119 18 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-11.76797244975028 120 1 -1.520968679495894 120 2 -0.8765252463218118 120 3 -1.349359919213029 120 4 -1.4204968373556 120 5 -0.292900695274696 120 6 -1.0249173662514437 120 7 -1.9628778416786332 120 8 -1.551873328416569 120 9 -1.4815185609964843 120 10 -2.0680465862162336 120 11 -5.024948204314445 120 12 -9.713474151794276 120 13 -11.12638146167805 120 14 -15.456025527523934 120 15 -16.795243888400528 120 16 -20.462561099546093 120 17 -18.948625810614054 120 18 -20.116102407089567 120 19 -6.534039737681454 120 20 -13.804070124341042 120 21 -7.094289475503821 120 22 -6.971822469743877 120 23 -4.350332410124203 120 24 -9.790057582581948 120 25 -5.174854453552793 120 26 -0.4430782023477349 120 27 0.4506947651648119 120 28 -1.2623611676760191 120 29 -1.0140851038938195 120 30 -1.6558767990595626 120 31 -3.9208534031489712 120 32 -12.979395296740908 120 33 -1.3654051887449654 120 34 -1.5271796148815042 120 35 -2.132778989277611 120 36 -0.6175563854177923 120 37 -0.6060250768408366 120 38 -0.98225761330648 120 39 -0.011148499299054038 120 40 4.908898678886278 120 41 0.8447984024805884 120 42 -6.187445636664801 120 43 -10.21628880477978 120 44 -9.200607248862159 120 45 -10.968661749700562 120 46 -16.419657656167246 120 47 -19.690789258285562 120 48 -16.385584530415095 120 49 -20.36265816161102 120 50 -17.8681317455119 120 51 -17.584986662850426 120 52 -17.60853805855922 120 53 -18.142895908088704 120 54 -20.736751609639647 120 55 -18.011466453292094 120 56 -16.169531903941312 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 55.0 5 10.0 6 21.0 7 47.0 8 46.0 9 42.0 10 30.0 11 28.0 12 18.0 13 28.0 14 33.0 15 60.0 16 66.0 17 98.0 18 129.0 19 144.0 20 170.0 21 198.0 22 283.0 23 351.0 24 417.0 25 492.0 26 520.0 27 589.0 28 686.0 29 892.0 30 1055.0 31 1196.0 32 1437.0 33 1694.0 34 1822.0 35 2242.0 36 2863.0 37 3923.0 38 3926.0 39 2551.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.2331996165998 0.6141503070751535 0.028400014200007102 0.12425006212503106 8 99.27224963612483 0.5289502644751323 0.07455003727501863 0.12425006212503106 9 95.95299797649899 2.2507011253505627 0.8910504455252228 0.9052504526252263 10 32.042316021158015 45.14182257091128 12.63445631722816 10.181405090702546 11 27.991763995881996 39.390819695409846 19.290709645354823 13.326706663353333 12 40.782420391210195 16.237708118854062 18.36060918030459 24.619262309631154 13 24.48965101004722 18.35126211524124 24.823374871303297 32.33571200340825 14 24.105367793240557 24.435529679068445 26.36324907696677 25.095853450724228 15 28.48601323937647 19.638408427646095 26.0872659975799 25.78831233539754 16 28.94989168649455 21.85446926382329 25.4696544621613 23.725984587520863 17 26.72086336043168 23.596861798430897 23.745961872980935 25.936312968156482 18 24.551812275906137 26.20256310128155 24.065462032731016 25.180162590081295 19 23.21301691061532 28.84041494955237 23.252096063663494 24.69447207616882 20 24.856086987420937 28.157202757444388 22.578352640181933 24.408357614952738 21 24.313962156981077 24.07966203983102 24.92456246228123 26.68181334090667 22 26.301963150981578 25.50676275338138 22.297561148780574 25.893712946856475 23 25.442862721431357 27.24626362313181 25.151762575881285 22.15911107955554 24 23.39806169903085 27.338563669281836 27.08296354148177 22.180411090205546 25 26.848663424331715 23.689161844580923 28.939614469807236 20.52256026128013 26 21.559926157341664 24.332575972735018 33.06589037205339 21.041607497869926 27 22.491558556957525 20.970321663408566 35.79171850008886 20.74640127954505 28 21.632769165125872 23.488835158583417 37.72223012373773 17.15616555255298 29 19.617870587399675 22.036366219191702 41.284892392925634 17.060870800482988 30 17.069620477864166 20.6802286363475 46.82429793730252 15.425852948485815 31 14.891879416255371 23.22905940418279 48.42523878848134 13.453822391080495 32 17.037379192723137 19.219016486640136 49.44215463331438 14.301449687322343 33 14.33245607795718 20.029874101998722 51.94892951134504 13.688740308699055 34 12.136818924486752 20.775023087305534 54.95489095688002 12.133267031327698 35 11.617829870360504 19.943171727934647 58.13177055585154 10.307227845853312 36 12.119598025638293 21.380632790028763 55.92841163310962 10.571357551223324 37 11.613017961942024 19.960874977769873 57.00160056909124 11.42450649119687 38 13.112068658523556 19.664541861044835 57.38755742316869 9.835832057262918 39 12.343922307993312 18.949877272242183 55.54409305965636 13.162107360108145 40 8.815949602691674 16.239530388717878 51.020115971078816 23.924404037511632 41 9.318752240946576 14.962352097525994 47.626389386877015 28.092506274650415 42 12.707948243992606 19.6182283520546 56.8534053746623 10.820418029290488 43 9.209636667734244 17.234262125902994 57.17590121347995 16.380199992882815 44 9.693076374018558 18.383297644539613 53.80085653104925 18.122769450392575 45 11.283839066557576 20.81391626053858 58.12315463697485 9.779090035928997 46 11.262895766631093 20.273923870508717 55.78797580932052 12.675204553539665 47 9.937138189437796 21.28777923784494 56.295059843023054 12.480022729694214 48 10.283397930519504 22.7393948014081 56.29555879529211 10.681648472780287 49 8.576935432059107 19.195488453438983 49.773351893493235 22.454224221008673 50 11.182994454713494 22.607706526375658 55.80833214844305 10.400966870467794 51 11.702279202279202 24.11680911680912 54.04558404558405 10.135327635327636 52 11.555824121138876 23.548857213948033 55.02790317420823 9.867415490704866 53 11.080687031044416 23.889619857046334 55.06916539241137 9.960527719497884 54 9.576512455516015 24.26690391459075 50.90035587188611 15.256227758007118 55 11.067629584830481 25.14141378206268 54.064534490732505 9.726422142374329 56 12.79516358463727 25.234708392603128 51.635846372688476 10.334281650071125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 2.0 7 3.0 8 8.0 9 13.0 10 34.0 11 55.0 12 104.0 13 173.5 14 194.0 15 262.5 16 331.0 17 466.5 18 602.0 19 630.5 20 659.0 21 769.5 22 1121.5 23 1363.0 24 1651.5 25 1940.0 26 2117.0 27 2294.0 28 2369.5 29 2330.5 30 2216.0 31 1881.0 32 1546.0 33 1302.5 34 1059.0 35 840.0 36 621.0 37 598.5 38 520.5 39 465.0 40 436.0 41 407.0 42 403.5 43 400.0 44 502.5 45 605.0 46 631.5 47 684.5 48 711.0 49 698.0 50 685.0 51 722.0 52 759.0 53 761.5 54 759.5 55 755.0 56 744.5 57 734.0 58 777.0 59 820.0 60 782.5 61 745.0 62 717.0 63 621.5 64 554.0 65 504.0 66 454.0 67 396.5 68 339.0 69 311.5 70 284.0 71 215.0 72 128.0 73 110.0 74 92.0 75 74.0 76 53.0 77 32.0 78 25.5 79 12.0 80 5.0 81 4.0 82 3.0 83 1.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.007100003550001776 14 0.003550001775000888 15 0.252050126025063 16 0.035500017750008876 17 0.0 18 0.0 19 0.07455003727501863 20 0.09585004792502395 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.003550001775000888 27 0.12070006035003018 28 0.15975007987503995 29 0.03905001952500976 30 0.007100003550001776 31 0.021300010650005325 32 0.08875004437502218 33 0.18105009052504525 34 0.05325002662501332 35 0.04970002485001243 36 0.028400014200007102 37 0.1917000958500479 38 0.3124001562000781 39 0.20590010295005148 40 0.8200504100252051 41 0.9904504952252476 42 0.13135006567503285 43 0.24140012070006037 44 0.5289502644751323 45 0.20590010295005148 46 0.20945010472505238 47 0.04260002130001065 48 0.1633000816500408 49 0.5396002698001349 50 0.13135006567503285 51 0.31595015797507897 52 0.12780006390003196 53 0.1704000852000426 54 0.24495012247506123 55 0.21300010650005327 56 0.1739500869750435 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 28169.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.24228762114382 #Duplication Level Percentage of deduplicated Percentage of total 1 86.6761028544468 65.2170826085413 2 7.43099787685775 11.182505591252797 3 2.561924982307148 5.782952891476445 4 1.1936777541873083 3.5926017963008983 5 0.6605331446095777 2.4850012425006214 6 0.3538570417551309 1.5975007987503995 7 0.2642132578438311 1.391600695800348 8 0.1934418494928049 1.1644005822002912 9 0.17928756782259966 1.2141006070503035 >10 0.47652748289690966 5.619652809826405 >50 0.004718093890068412 0.23785011892505947 >100 0.004718093890068412 0.5147502573751287 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 145 0.5147502573751287 No Hit CATGATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67 0.23785011892505947 No Hit CATGATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG 42 0.14910007455003726 No Hit CATGATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.1420000710000355 No Hit CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 40 0.1420000710000355 No Hit CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 39 0.1384500692250346 No Hit CATGATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 39 0.1384500692250346 No Hit CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 38 0.13490006745003372 No Hit CATGATGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37 0.13135006567503285 No Hit CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 34 0.12070006035003018 No Hit CATGATGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31 0.11005005502502752 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.017750008875004438 0.0 0.0 0.0 0.0 11 0.024850012425006212 0.0 0.0 0.0 0.0 12 0.03195001597500799 0.0 0.0 0.0 0.0 13 0.03195001597500799 0.0 0.0 0.0 0.0 14 0.035500017750008876 0.0 0.0 0.0 0.0 15 0.03905001952500976 0.0 0.0 0.0 0.0 16 0.04260002130001065 0.0 0.0 0.0 0.0 17 0.04615002307501154 0.0 0.0 0.0 0.0 18 0.04615002307501154 0.0 0.0 0.0 0.0 19 0.05325002662501331 0.0 0.0 0.0 0.0 20 0.0568000284000142 0.0 0.0 0.0 0.0 21 0.0568000284000142 0.0 0.0 0.0 0.0 22 0.06390003195001598 0.0 0.0 0.0 0.0 23 0.0816500408250204 0.0 0.0 0.0 0.0 24 0.09230004615002307 0.0 0.0 0.0 0.0 25 0.09940004970002485 0.0 0.0 0.0 0.0 26 0.12070006035003017 0.0 0.0 0.0 0.0 27 0.1420000710000355 0.0 0.0 0.0 0.0 28 0.1633000816500408 0.0 0.0 0.0 0.0 29 0.1739500869750435 0.0 0.0 0.0 0.0 30 0.18815009407504704 0.0 0.0 0.0 0.0 31 0.18815009407504704 0.0 0.0 0.0 0.0 32 0.18815009407504704 0.0 0.0 0.0 0.0 33 0.18815009407504704 0.0 0.0 0.0 0.0 34 0.1988000994000497 0.0 0.0 0.0 0.0 35 0.20590010295005148 0.0 0.0 0.0 0.0 36 0.22010011005005503 0.0 0.0 0.0 0.0 37 0.22365011182505593 0.0 0.0 0.0 0.0 38 0.2272001136000568 0.0 0.0 0.0 0.0 39 0.2272001136000568 0.0 0.0 0.0 0.0 40 0.2272001136000568 0.0 0.0 0.0 0.0 41 0.23075011537505768 0.0 0.0 0.0 0.0 42 0.23785011892505947 0.0 0.0 0.0 0.0 43 0.24140012070006034 0.0 0.0 0.0 0.0 44 0.24850012425006213 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTAGC 15 0.008121855 50.344704 41 CCGTTGT 15 0.008121855 50.344704 38 CGTTGTA 15 0.008121855 50.344704 39 GTTGTAG 15 0.008121855 50.344704 40 TATGCTT 20 4.0475128E-4 50.16458 35 ATGCTTG 20 4.0475128E-4 50.16458 36 ATATGCT 20 4.0475128E-4 50.16458 34 TAGCGCC 15 0.008238047 50.164577 44 ACCCGTT 15 0.008238047 50.164577 36 GCTACCC 15 0.008238047 50.164577 33 CCCGTTG 15 0.008238047 50.164577 37 TGTAGCG 15 0.008238047 50.164577 42 TACCCGT 15 0.008238047 50.164577 35 AGCGCCG 15 0.008238047 50.164577 45 AGATTAA 25 1.9959843E-5 50.164577 49 CATATGC 25 1.9959843E-5 50.164577 33 CTACCCG 15 0.008238047 50.164577 34 TGCTACC 15 0.008296605 50.074997 32 GCATATG 25 2.0172936E-5 50.074997 32 CCAGTAG 30 1.0134299E-6 49.98574 26 >>END_MODULE