FastQCFastQC Report
Fri 17 Jun 2016
SRR1526233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7152
Sequences flagged as poor quality0
Sequence length56
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.3215883668903803No Hit
GGCTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG180.25167785234899326No Hit
GGCTGCGGGTAACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.23769574944071586No Hit
GGCTGCGGGATGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.22371364653243847No Hit
GGCTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC160.22371364653243847No Hit
GGCTGCGGGAAGCATGTACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT150.20973154362416108No Hit
GGCTGCGGGAGCATGGTCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.16778523489932887No Hit
GGCTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA120.16778523489932887No Hit
GGCTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA120.16778523489932887No Hit
GGCTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA110.15380313199105144No Hit
GGCTGCGGGAAGATGGACACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT90.12583892617449663No Hit
GGCTGCGGGGCTGGCTGCGGGAAGCATGGTATCAACGCAGAGTTTTTTTTTTTTTT90.12583892617449663No Hit
GGCTGCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG90.12583892617449663No Hit
GGCTGCGGGAGAGCACCAGGAGCTCAGGGTCCGGTTGGGCAGCTGAGTGCCATTCG90.12583892617449663No Hit
GGCTGCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGAG80.11185682326621924No Hit
GGCTGCGGGAAAGTGTCAACGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT80.11185682326621924No Hit
GGCTGCGGGATGGAACGCAGAGTCGACTTTTTTTTTTTTTCTTTTTTTTTTTTTTT80.11185682326621924No Hit
GGCTGCGGGAACATTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT80.11185682326621924No Hit
GGCTGCGGGACTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.11185682326621924No Hit
GGCTGCGGGGTTTTCAAGGCCTGTTAAAATTCTGTTGTATATAGTAGTTTTTGAGC80.11185682326621924No Hit
GGCTGCGGGAAGAGTACACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.11185682326621924No Hit
GGCTGCGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTC80.11185682326621924No Hit
GGCTGCGGGGCTGGCTGCGGGAAGCATGGTATCAACGCAGCGTTTTTTTTTTTTTT80.11185682326621924No Hit
GGCTGCGGGACGCAGAGTCGACTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTT80.11185682326621924No Hit
GGCTGCGGGTACGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.11185682326621924No Hit
GGCTGCGGGAAGAGTGGTATCAACCAGAATCGACTTTTTTTTTTTTTTTTTTTTTT80.11185682326621924No Hit
GGCTGCGGGAGATCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.11185682326621924No Hit
GGCTGCGGGAGAGTGGAAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT80.11185682326621924No Hit
GGCTGCGGCCCAGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.11185682326621924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTGT150.00793202250.2535232
GCGGGGG750.049.90215
GCGGGCT451.0186341E-1049.90215
GCGGGCA150.00815754349.90215
GCGGGAT600.049.90215
GCGGGAG850.049.90215
ACAAAAC150.00815754349.902148
GAGTGGT150.00815754349.90219
CGGGGTG150.00815754349.90216
GGGTATC150.00815754349.90217
CGGGGGT150.00815754349.90216
CGGGGGG251.9069104E-549.90216
CGGGGGC251.9069104E-549.90216
CGGGGAC150.00815754349.90216
CGGGCTG354.436697E-849.90216
CGGGTAT150.00815754349.90216
CGGGATG251.9069104E-549.90216
CGGGACT150.00815754349.90216
GGGAAGA251.9069104E-549.90217
GGGGAGT150.00815754349.90217
Produced by FastQC (version 0.11.5)