##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526233_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7152 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.0673937360179 39.0 38.0 39.0 35.0 40.0 2 37.82242729306488 39.0 38.0 39.0 35.0 40.0 3 37.90590044742729 39.0 38.0 39.0 35.0 40.0 4 37.89485458612975 39.0 38.0 39.0 35.0 40.0 5 37.641498881431765 39.0 38.0 39.0 35.0 40.0 6 37.458612975391496 39.0 38.0 39.0 35.0 40.0 7 37.58123601789709 39.0 38.0 39.0 35.0 40.0 8 37.70064317673378 39.0 38.0 39.0 35.0 40.0 9 37.75615212527964 39.0 38.0 39.0 35.0 40.0 10 37.1282158836689 39.0 36.0 39.0 33.0 40.0 11 35.64918903803132 38.0 35.0 39.0 29.0 40.0 12 34.18847874720358 38.0 33.0 39.0 22.0 40.0 13 33.816275167785236 38.0 33.0 39.0 21.0 40.0 14 33.96364653243848 38.0 33.0 39.0 22.0 39.0 15 33.98895413870246 38.0 33.0 39.0 22.0 39.0 16 33.422818791946305 37.0 32.0 39.0 22.0 39.0 17 33.43176733780761 37.0 31.0 38.0 22.0 39.0 18 32.70973154362416 36.0 30.0 38.0 19.0 39.0 19 33.81515659955257 37.0 32.0 38.0 23.0 39.0 20 33.973993288590606 37.0 32.0 38.0 25.0 39.0 21 34.49035234899329 38.0 33.0 39.0 25.0 39.0 22 33.9675615212528 37.0 33.0 38.0 23.0 39.0 23 34.45665548098434 38.0 33.0 39.0 26.0 40.0 24 34.096196868008946 38.0 33.0 39.0 24.0 39.0 25 34.324384787472034 38.0 33.0 39.0 25.0 39.0 26 34.74217002237136 38.0 34.0 39.0 27.0 39.0 27 34.724272930648766 38.0 34.0 39.0 27.0 39.0 28 34.665548098434 38.0 33.0 39.0 27.0 39.0 29 34.65813758389262 38.0 34.0 39.0 27.0 39.0 30 34.43638143176734 38.0 33.0 39.0 25.0 39.0 31 34.830397091722595 38.0 35.0 39.0 27.0 39.0 32 34.71462527964206 38.0 35.0 39.0 27.0 39.0 33 34.547539149888145 38.0 34.0 39.0 26.0 39.0 34 34.54404362416108 38.0 34.0 39.0 26.0 39.0 35 34.48000559284117 38.0 34.0 39.0 26.0 39.0 36 33.955257270693515 37.0 33.0 39.0 26.0 39.0 37 33.703719239373605 36.0 33.0 38.0 25.0 39.0 38 33.28467561521253 36.0 32.0 38.0 24.0 39.0 39 32.76901565995526 35.0 31.0 38.0 20.0 39.0 40 29.582774049217 35.0 24.0 38.0 9.0 39.0 41 26.372063758389263 31.0 15.0 38.0 5.0 39.0 42 30.60444630872483 35.0 29.0 38.0 11.0 39.0 43 29.20525727069351 34.0 26.0 38.0 5.0 39.0 44 29.11143736017897 34.0 26.0 38.0 4.0 39.0 45 30.310822147651006 35.0 29.0 38.0 4.0 39.0 46 30.083752796420583 35.0 27.0 38.0 4.0 39.0 47 29.48140380313199 35.0 27.0 38.0 4.0 39.0 48 29.659955257270692 35.0 27.0 38.0 4.0 39.0 49 27.119826621923938 34.0 16.0 38.0 4.0 39.0 50 29.177572706935123 35.0 25.0 38.0 4.0 39.0 51 29.444211409395972 35.0 27.0 39.0 4.0 39.0 52 29.2337807606264 35.0 26.0 39.0 4.0 39.0 53 28.846057046979865 35.0 25.0 39.0 4.0 39.0 54 27.82172818791946 35.0 18.0 39.0 4.0 39.0 55 27.98937360178971 35.0 21.0 39.0 4.0 39.0 56 26.756291946308725 33.0 17.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.30821759259259096 1 2 0.13023478835978608 1 3 -2.2216798941798928 1 4 -0.2881415343915421 1 5 0.10631283068783404 1 6 -0.28491732804234005 1 7 0.42581349206347596 1 8 0.5470998677248673 1 9 0.30769179894181775 1 10 -0.7348379629629491 1 11 -4.310492724867736 1 12 -16.531296296296286 1 13 -13.712076719576725 1 14 -6.313230820105819 1 15 -4.223763227513224 1 16 -24.037466931216947 1 17 -3.2258399470899626 1 18 -15.037103174603178 1 19 -3.4995767195767122 1 20 -5.814454365079378 1 21 -7.4689285714285845 1 22 -6.728750000000002 1 23 -4.182433862433868 1 24 -3.429279100529101 1 25 -5.53836309523809 1 26 2.7886375661375666 1 27 2.1399140211640244 1 28 2.7913293650793705 1 29 2.02448743386244 1 30 4.208465608465623 1 31 2.736008597883597 1 32 3.571541005291003 1 33 3.7675396825396774 1 34 1.9391832010582064 1 35 4.193197751322742 1 36 4.206646825396831 1 37 4.911521164021174 1 38 5.207328042328037 1 39 5.276107804232797 1 40 9.321471560846557 1 41 -6.644113756613756 1 42 -6.252863756613767 1 43 -14.962850529100521 1 44 -21.47508597883598 1 45 -4.758250661375655 1 46 -10.311865079365084 1 47 -22.43779100529102 1 48 -8.844560185185198 1 49 -18.576593915343917 1 50 -7.966097883597872 1 51 -2.5056746031746044 1 52 -1.73054894179894 1 53 -2.000631613756614 1 54 -1.1839087301587377 1 55 -0.6519973544973539 1 56 4.713458994708997 2 1 0.8251157407407419 2 2 1.5635681216931232 2 3 1.3227645502645515 2 4 1.3674140211640164 2 5 1.4396461640211697 2 6 1.7484160052909985 2 7 1.859146825396813 2 8 1.8693220899470901 2 9 1.6521362433862592 2 10 1.3540509259259395 2 11 2.6339517195767073 2 12 0.10203703703704292 2 13 3.6545899470899457 2 14 5.120102513227515 2 15 4.82068121693122 2 16 3.506977513227497 2 17 4.763048941798928 2 18 -0.29265873015873467 2 19 3.689312169312174 2 20 3.685545634920622 2 21 3.2310714285714184 2 22 2.5379166666666677 2 23 3.373121693121689 2 24 1.770720899470902 2 25 2.483859126984129 2 26 4.210859788359791 2 27 4.85102513227514 2 28 4.346884920634928 2 29 5.0578207671957784 2 30 4.452910052910063 2 31 3.3693419312169297 2 32 2.2270965608465616 2 33 2.7008730158730145 2 34 3.2502943121693235 2 35 4.215419973544961 2 36 3.5955357142857167 2 37 4.967076719576731 2 38 3.6628835978835923 2 39 6.009441137566132 2 40 6.8214715608465575 2 41 -6.6885582010582 2 42 -4.6861970899471 2 43 -12.2406283068783 2 44 -10.008419312169313 2 45 -5.036028439153434 2 46 -6.534087301587306 2 47 -14.871124338624353 2 48 -4.955671296296309 2 49 -8.78770502645503 2 50 -3.143875661375649 2 51 -0.5390079365079359 2 52 -0.6749933862433828 2 53 -0.10063161375661522 2 54 -1.0616865079365176 2 55 1.5924470899470897 2 56 4.046792328042329 3 1 0.5965443121693141 3 2 1.0349966931216912 3 3 0.7227645502645501 3 4 0.5531283068782997 3 5 0.08250330687831564 3 6 1.119844576719565 3 7 0.9020039682539576 3 8 1.483607804232804 3 9 0.82356481481483 3 10 0.9397652116402213 3 11 0.7053802910052767 3 12 3.873465608465615 3 13 4.383161375661373 3 14 4.591531084656083 3 15 1.5778240740740728 3 16 4.76412037037035 3 17 3.3059060846560726 3 18 4.907341269841265 3 19 4.4607407407407464 3 20 3.685545634920622 3 21 4.0310714285714155 3 22 1.5093452380952357 3 23 3.373121693121689 3 24 3.6421494708994686 3 25 3.898144841269847 3 26 2.7965740740740728 3 27 1.965310846560854 3 28 2.7040277777777817 3 29 2.8578207671957756 3 30 2.4386243386243507 3 31 2.4264847883597866 3 32 2.4842394179894143 3 33 2.3151587301587284 3 34 1.764580026455036 3 35 1.3439914021163943 3 36 1.9526785714285708 3 37 2.5385052910053005 3 38 3.5485978835978784 3 39 3.6808697089947024 3 40 9.392900132275127 3 41 9.197156084656086 3 42 5.913802910052901 3 43 0.6879431216931273 3 44 2.5487235449735444 3 45 3.6782572751322817 3 46 -2.462658730158733 3 47 -3.3425529100529268 3 48 1.3300429894179757 3 49 3.66943783068783 3 50 1.3275529100529226 3 51 3.8752777777777787 3 52 2.2535780423280443 3 53 0.7850826719576709 3 54 2.038313492063484 3 55 0.07816137566137371 3 56 2.0467923280423292 4 1 1.1501157407407447 4 2 0.8385681216931218 4 3 0.9727645502645501 4 4 1.392414021164015 4 5 1.6896461640211697 4 6 1.6734160052909957 4 7 1.3841468253968117 4 8 1.5193220899470887 4 9 1.7521362433862606 4 10 2.2790509259259366 4 11 4.133951719576707 4 12 5.927037037037046 4 13 6.079589947089943 4 14 5.020102513227513 4 15 4.970681216931219 4 16 6.0319775132274955 4 17 6.288048941798927 4 18 7.032341269841265 4 19 6.014312169312177 4 20 4.810545634920622 4 21 3.6560714285714155 4 22 5.312916666666666 4 23 5.373121693121689 4 24 3.945720899470899 4 25 5.058859126984132 4 26 4.010859788359788 4 27 4.001025132275139 4 28 4.096884920634928 4 29 5.357820767195776 4 30 5.152910052910066 4 31 3.3193419312169326 4 32 4.12709656084656 4 33 4.225873015873013 4 34 3.4252943121693207 4 35 3.7904199735449637 4 36 4.720535714285717 4 37 5.717076719576731 4 38 5.012883597883594 4 39 7.359441137566133 4 40 10.696471560846557 4 41 12.661441798941802 4 42 8.663802910052901 4 43 10.7593716931217 4 44 9.691580687830687 4 45 9.088971560846566 4 46 9.215912698412694 4 47 9.728875661375646 4 48 3.919328703703691 4 49 8.437294973544972 4 50 4.25612433862435 4 51 5.285992063492063 4 52 5.700006613756617 4 53 7.249368386243386 4 54 7.538313492063484 4 55 6.792447089947089 4 56 7.671792328042329 5 1 0.9251157407407433 5 2 1.6635681216931246 5 3 1.422764550264553 5 4 1.4674140211640179 5 5 1.6396461640211726 5 6 1.9484160052909942 5 7 0.05914682539680882 5 8 1.6693220899470873 5 9 0.45213624338626346 5 10 -0.5459490740740662 5 11 3.033951719576706 5 12 4.302037037037046 5 13 5.454589947089943 5 14 3.920102513227512 5 15 4.4206812169312215 5 16 5.9069775132274955 5 17 6.463048941798924 5 18 4.6073412698412675 5 19 5.789312169312176 5 20 5.385545634920625 5 21 3.8310714285714127 5 22 4.337916666666665 5 23 4.473121693121691 5 24 5.270720899470902 5 25 4.683859126984132 5 26 3.710859788359791 5 27 4.251025132275139 5 28 2.8468849206349276 5 29 4.157820767195773 5 30 2.852910052910069 5 31 3.469341931216931 5 32 3.0270965608465588 5 33 3.300873015873016 5 34 1.0502943121693207 5 35 3.715419973544961 5 36 2.2955357142857196 5 37 4.067076719576733 5 38 5.162883597883592 5 39 2.2094411375661345 5 40 6.02147156084656 5 41 8.611441798941804 5 42 5.213802910052898 5 43 6.459371693121703 5 44 6.291580687830688 5 45 5.963971560846566 5 46 2.1659126984126935 5 47 2.6288756613756448 5 48 2.6443287037036924 5 49 1.112294973544973 5 50 0.15612433862434827 5 51 -0.439007936507938 5 52 -0.4749933862433835 5 53 0.6993683862433855 5 54 1.9383134920634824 5 55 0.6924470899470876 5 56 -1.2532076719576715 6 1 1.1251157407407462 6 2 1.3635681216931204 6 3 1.1227645502645487 6 4 0.6674140211640136 6 5 1.739646164021167 6 6 1.8484160052909928 6 7 1.7591468253968117 6 8 1.4693220899470916 6 9 1.7521362433862606 6 10 1.1540509259259366 6 11 3.7339517195767087 6 12 5.302037037037046 6 13 5.354589947089941 6 14 4.420102513227512 6 15 4.020681216931216 6 16 4.706977513227493 6 17 5.663048941798927 6 18 5.707341269841262 6 19 3.2893121693121756 6 20 3.7855456349206236 6 21 3.131071428571417 6 22 4.2379166666666634 6 23 3.9731216931216906 6 24 3.1707208994709006 6 25 3.483859126984129 6 26 2.1108597883597895 6 27 3.4510251322751415 6 28 3.8468849206349276 6 29 4.357820767195776 6 30 3.452910052910063 6 31 4.669341931216934 6 32 4.727096560846562 6 33 3.9008730158730103 6 34 3.9502943121693193 6 35 3.515419973544965 6 36 3.895535714285714 6 37 4.667076719576734 6 38 5.562883597883591 6 39 4.109441137566133 6 40 9.721471560846556 6 41 4.611441798941801 6 42 7.613802910052904 6 43 8.959371693121703 6 44 9.491580687830684 6 45 8.963971560846566 6 46 8.865912698412693 6 47 9.228875661375646 6 48 8.644328703703692 6 49 11.012294973544975 6 50 9.956124338624353 6 51 7.560992063492062 6 52 7.225006613756616 6 53 7.999368386243386 6 54 9.538313492063484 6 55 6.192447089947088 6 56 10.246792328042332 7 1 -1.0859953703703695 7 2 -1.7586541005290997 7 3 -0.6661243386243356 7 4 -0.5103637566137635 7 5 -3.2270205026454946 7 6 -0.7293617724867829 7 7 -2.3519642857143026 7 8 0.32487764550264586 7 9 -0.13675264550262511 7 10 -3.068171296296285 7 11 1.5783961640211501 7 12 2.8020370370370458 7 13 2.8434788359788286 7 14 2.242324735449735 7 15 5.109570105820104 7 16 2.7403108465608312 7 17 2.6630489417989267 7 18 3.2962301587301504 7 19 3.1670899470899556 7 20 4.407767857142844 7 21 3.5310714285714155 7 22 3.382361111111109 7 23 2.817566137566132 7 24 1.1262764550264563 7 25 0.6838591269841316 7 26 0.5664153439153452 7 27 1.8065806878306958 7 28 0.7913293650793705 7 29 3.135598544973554 7 30 3.9862433862434017 7 31 -1.2639914021164032 7 32 1.571541005291003 7 33 1.6564285714285703 7 34 1.0502943121693207 7 35 0.526531084656078 7 36 0.4288690476190524 7 37 0.5781878306878454 7 38 -2.348227513227517 7 39 -2.2794477513227562 7 40 0.6548048941798896 7 41 -6.199669312169309 7 42 0.5249140211640118 7 43 1.0371494708994788 7 44 0.5249140211640189 7 45 -2.8693617724867657 7 46 -3.2007539682539736 7 47 -1.326679894179911 7 48 -1.5112268518518661 7 49 0.6456283068783044 7 50 -1.1883201058200932 7 51 -0.7278968253968259 7 52 -0.9527711640211614 7 53 -0.11174272486772452 7 54 1.1494246031745945 7 55 0.792447089947089 7 56 1.713458994708997 8 1 1.136226851851859 8 2 1.6857903439153432 8 3 1.4449867724867715 8 4 0.8229695767195722 8 5 1.6618683862433912 8 6 1.6039715608465528 8 7 1.981369047619033 8 8 1.880433201058203 8 9 1.8632473544973749 8 10 1.4873842592592723 8 11 3.3561739417989287 8 12 3.8020370370370458 8 13 5.843478835978829 8 14 5.242324735449735 8 15 5.109570105820104 8 16 5.07364417989416 8 17 6.440826719576705 8 18 7.185119047619043 8 19 5.167089947089956 8 20 5.629990079365065 8 21 2.9755158730158584 8 22 4.049027777777781 8 23 3.0397883597883535 8 24 5.348498677248678 8 25 4.794970238095246 8 26 3.6775264550264524 8 27 3.0288029100529172 8 28 2.3468849206349276 8 29 3.46893187830689 8 30 2.9862433862434017 8 31 2.291564153439154 8 32 4.349318783068782 8 33 4.65642857142857 8 34 1.3836276455026564 8 35 4.526531084656078 8 36 4.53998015873016 8 37 2.6892989417989526 8 38 5.429550264550258 8 39 5.609441137566133 8 40 8.321471560846557 8 41 -0.9774470899470877 8 42 5.6360251322751225 8 43 7.481593915343922 8 44 7.858247354497351 8 45 7.241749338624345 8 46 6.1325793650793585 8 47 5.117764550264532 8 48 2.377662037037023 8 49 6.4234060846560865 8 50 5.811679894179907 8 51 5.049880952380949 8 52 5.602784391534396 8 53 1.8882572751322755 8 54 4.1494246031745945 8 55 2.0146693121693104 8 56 4.824570105820108 9 1 -0.5748842592592567 9 2 -1.236431878306881 9 3 -2.577235449735447 9 4 -1.8325859788359864 9 5 -0.3603538359788274 9 6 -0.6515839947090072 9 7 -0.24085317460318834 9 8 0.36932208994709015 9 9 0.5521362433862578 9 10 -3.5459490740740662 9 11 -0.6660482804232899 9 12 2.602037037037043 9 13 0.054589947089944246 9 14 2.7201025132275163 9 15 0.12068121693121725 9 16 1.7069775132274927 9 17 3.3630489417989295 9 18 2.1073412698412675 9 19 1.6893121693121742 9 20 -0.31445436507937785 9 21 -1.6689285714285838 9 22 0.13791666666666913 9 23 -1.7268783068783122 9 24 -1.5292791005291022 9 25 0.18385912698413165 9 26 -2.0891402116402134 9 27 0.05102513227513583 9 28 0.7468849206349262 9 29 -5.042179232804223 9 30 1.9529100529100631 9 31 -0.830658068783066 9 32 -0.5729034391534427 9 33 -2.9991269841269883 9 34 -7.149705687830679 9 35 -5.984580026455035 9 36 -2.304464285714282 9 37 -3.6329232804232703 9 38 -1.7371164021164063 9 39 -4.390558862433867 9 40 3.0214715608465568 9 41 4.111441798941801 9 42 1.413802910052901 9 43 -1.1406283068782983 9 44 2.891580687830686 9 45 1.5639715608465679 9 46 2.0659126984126956 9 47 3.228875661375646 9 48 -1.455671296296309 9 49 3.9122949735449737 9 50 -1.8438756613756517 9 51 2.560992063492062 9 52 4.625006613756614 9 53 3.1993683862433855 9 54 4.638313492063485 9 55 0.9924470899470883 9 56 3.7467923280423285 10 1 1.1251157407407462 10 2 1.1635681216931246 10 3 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6.98523148148147 114 43 -2.0263425925925844 114 44 -8.379847883597886 114 45 6.749685846560851 114 46 -0.10551587301587873 114 47 -1.0568386243386385 114 48 -0.6699570105820243 114 49 -17.259133597883597 114 50 -2.601018518518508 114 51 -1.6961507936507942 114 52 -1.3178505291005251 114 53 -3.143488756613756 114 54 -13.67597222222223 114 55 -6.493267195767196 114 56 -4.381779100529098 115 1 -0.9748842592592553 115 2 -1.1614318783068782 115 3 -3.65223544973545 115 4 -0.607585978835985 115 5 -0.9353538359788303 115 6 -2.7015839947090043 115 7 -2.8658531746031883 115 8 -2.8556779100529113 115 9 -2.8728637566137394 115 10 -1.0959490740740634 115 11 -3.7410482804232927 115 12 -11.197962962962954 115 13 -10.045410052910057 115 14 -8.354897486772487 115 15 -8.779318783068781 115 16 -8.468022486772504 115 17 -3.0869510582010733 115 18 -1.8426587301587354 115 19 -1.860687830687823 115 20 -2.189454365079378 115 21 3.5310714285714155 115 22 2.8129166666666663 115 23 2.373121693121689 115 24 3.195720899470899 115 25 2.8088591269841316 115 26 1.510859788359788 115 27 2.8760251322751387 115 28 3.5968849206349276 115 29 3.6078207671957756 115 30 3.652910052910066 115 31 -4.0556580687830674 115 32 -1.7479034391534398 115 33 -1.3991269841269869 115 34 -2.4497056878306793 115 35 -1.3345800264550363 115 36 0.3455357142857167 115 37 1.2170767195767311 115 38 -0.11211640211640628 115 39 1.359441137566133 115 40 3.5714715608465575 115 41 0.9114417989418016 115 42 -6.211197089947099 115 43 -6.7406283068783 115 44 -2.1834193121693133 115 45 -4.786028439153434 115 46 -7.659087301587306 115 47 -8.271124338624354 115 48 -6.955671296296309 115 49 -11.437705026455028 115 50 -7.11887566137565 115 51 -5.089007936507937 115 52 -5.674993386243383 115 53 -4.750631613756614 115 54 -7.711686507936516 115 55 -4.457552910052911 115 56 -0.7032076719576708 116 1 0.02511574074074474 116 2 0.21356812169312178 116 3 0.2227645502645501 116 4 0.14241402116401503 116 5 0.43964616402116974 116 6 1.0484160052909957 116 7 0.7591468253968117 116 8 0.6443220899470887 116 9 0.6271362433862606 116 10 0.2790509259259366 116 11 -0.8660482804232927 116 12 -13.572962962962954 116 13 -9.920410052910057 116 14 -1.7298974867724866 116 15 -13.279318783068781 116 16 -3.7180224867725045 116 17 -1.9619510582010733 116 18 -8.342658730158735 116 19 -3.360687830687823 116 20 -2.189454365079378 116 21 3.7810714285714155 116 22 2.6879166666666663 116 23 3.248121693121689 116 24 2.570720899470899 116 25 2.3088591269841316 116 26 3.635859788359788 116 27 3.6260251322751387 116 28 2.8468849206349276 116 29 3.9828207671957756 116 30 4.027910052910066 116 31 2.8193419312169326 116 32 3.37709656084656 116 33 0.4758730158730131 116 34 2.9252943121693207 116 35 3.4154199735449637 116 36 3.2205357142857167 116 37 4.092076719576731 116 38 3.7628835978835937 116 39 4.859441137566133 116 40 7.5714715608465575 116 41 4.9114417989418016 116 42 0.28880291005290104 116 43 -15.2406283068783 116 44 -11.558419312169313 116 45 -1.0360284391534336 116 46 -3.9090873015873058 116 47 -1.3961243386243538 116 48 -1.455671296296309 116 49 -17.937705026455028 116 50 -1.4938756613756503 116 51 -1.9640079365079366 116 52 -1.1749933862433828 116 53 -2.500631613756614 116 54 -16.086686507936516 116 55 0.792447089947089 116 56 1.6717923280423292 117 1 0.02511574074074474 117 2 0.4635681216931218 117 3 0.2227645502645501 117 4 0.267414021164015 117 5 0.43964616402116974 117 6 1.0484160052909957 117 7 0.7591468253968117 117 8 0.5193220899470887 117 9 0.7521362433862606 117 10 1.1540509259259366 117 11 -0.36604828042329274 117 12 -8.447962962962954 117 13 -10.295410052910057 117 14 -3.2298974867724866 117 15 -6.529318783068781 117 16 -15.593022486772504 117 17 -6.586951058201073 117 18 -8.092658730158735 117 19 -1.860687830687823 117 20 -5.064454365079378 117 21 0.031071428571415538 117 22 0.6879166666666663 117 23 1.6231216931216892 117 24 1.5707208994708992 117 25 1.9338591269841316 117 26 1.510859788359788 117 27 3.2510251322751387 117 28 3.8468849206349276 117 29 2.6078207671957756 117 30 0.652910052910066 117 31 0.5693419312169326 117 32 0.8770965608465602 117 33 -0.6491269841269869 117 34 2.3002943121693207 117 35 1.6654199735449637 117 36 5.095535714285717 117 37 5.217076719576731 117 38 5.012883597883594 117 39 4.359441137566133 117 40 9.821471560846557 117 41 7.1614417989418016 117 42 2.913802910052901 117 43 -11.4906283068783 117 44 -14.308419312169313 117 45 3.4639715608465664 117 46 -1.5340873015873058 117 47 0.2288756613756462 117 48 -2.455671296296309 117 49 -8.937705026455028 117 50 0.00612433862434969 117 51 6.160992063492063 117 52 5.075006613756617 117 53 3.999368386243386 117 54 -3.711686507936516 117 55 6.792447089947089 117 56 9.54679232804233 118 1 -0.30821759259259096 118 2 -0.3697652116402139 118 3 -0.11056878306878559 118 4 -0.3992526455026493 118 5 0.10631283068783404 118 6 0.71508267195766 118 7 -1.074186507936524 118 8 -0.39734457671957557 118 9 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2.52448743386244 119 30 4.652910052910066 119 31 1.7360085978835968 119 32 -1.3729034391534398 119 33 4.100873015873013 119 34 3.5502943121693207 119 35 4.415419973544964 119 36 5.095535714285717 119 37 4.967076719576731 119 38 5.262883597883594 119 39 6.109441137566133 119 40 9.821471560846557 119 41 7.4114417989418016 119 42 2.913802910052901 119 43 -5.7406283068783 119 44 -5.308419312169313 119 45 3.797304894179902 119 46 -4.200753968253974 119 47 -7.104457671957686 119 48 -4.622337962962977 119 49 -9.687705026455028 119 50 2.9227910052910175 119 51 4.327658730158728 119 52 3.1583399470899494 119 53 -2.000631613756614 119 54 -10.461686507936516 119 55 2.625780423280421 119 56 7.046792328042329 120 1 -1.3748842592592538 120 2 -0.5364318783068782 120 3 -0.17723544973544847 120 4 -0.13258597883598355 120 5 0.03964616402117116 120 6 0.048416005290995656 120 7 -0.040853174603185494 120 8 -0.6306779100529099 120 9 -0.04786375661373654 120 10 -0.24594907407406197 120 11 -5.266048280423291 120 12 -9.197962962962954 120 13 -11.845410052910058 120 14 -20.379897486772485 120 15 -21.379318783068783 120 16 -13.593022486772504 120 17 -16.336951058201073 120 18 -14.192658730158737 120 19 -6.0106878306878215 120 20 -17.014454365079377 120 21 -5.068928571428586 120 22 -0.6620833333333351 120 23 -1.82687830687831 120 24 -4.029279100529102 120 25 0.08385912698413023 120 26 1.210859788359791 120 27 -2.14897486772486 120 28 2.5468849206349304 120 29 -1.2421792328042258 120 30 1.0529100529100646 120 31 1.0693419312169326 120 32 -13.472903439153441 120 33 0.10087301587301312 120 34 -0.7497056878306765 120 35 0.41541997354496374 120 36 0.09553571428571672 120 37 -2.3329232804232696 120 38 -0.037116402116403435 120 39 2.0094411375661316 120 40 2.5214715608465568 120 41 8.3114417989418 120 42 -3.9861970899470975 120 43 0.8593716931217017 120 44 1.9915806878306874 120 45 -3.136028439153435 120 46 -4.3340873015873065 120 47 -5.971124338624353 120 48 0.4443287037036896 120 49 -14.487705026455028 120 50 0.6561243386243483 120 51 -3.039007936507936 120 52 -6.174993386243383 120 53 -3.000631613756614 120 54 -5.1616865079365155 120 55 -3.4075529100529103 120 56 3.0467923280423292 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 11.0 5 2.0 6 5.0 7 2.0 8 7.0 9 24.0 10 7.0 11 13.0 12 10.0 13 14.0 14 8.0 15 16.0 16 22.0 17 26.0 18 36.0 19 48.0 20 39.0 21 59.0 22 84.0 23 110.0 24 120.0 25 140.0 26 139.0 27 162.0 28 210.0 29 216.0 30 300.0 31 376.0 32 389.0 33 499.0 34 551.0 35 657.0 36 806.0 37 975.0 38 794.0 39 274.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.81152125279642 0.6851230425055929 0.22371364653243847 0.2796420581655481 8 99.09116331096197 0.5453020134228188 0.25167785234899326 0.11185682326621924 9 96.56040268456377 1.524049217002237 0.9228187919463088 0.9927293064876959 10 39.90492170022371 38.82829977628635 10.109060402684564 11.15771812080537 11 26.272371364653246 31.711409395973156 19.001677852348994 23.014541387024607 12 32.10290827740492 16.484899328859058 27.572706935123044 23.839485458612973 13 33.44986715144735 18.36106838204447 21.98293944902811 26.206125017480076 14 29.92170022371365 22.553131991051455 23.53187919463087 23.993288590604024 15 25.893733352025794 17.524183373054818 23.81887004065611 32.76321323426328 16 27.266368214885283 21.89423614997202 28.063794068270848 22.775601566871853 17 33.43120805369127 20.875279642058164 20.693512304250557 25.0 18 25.083892617449667 22.69295302013423 21.015100671140942 31.20805369127517 19 32.857542681220266 25.552756787013713 16.70864819479429 24.88105233697173 20 32.65220433869839 26.18614415675297 19.34219734079776 21.819454163750876 21 32.13087248322148 23.252237136465325 19.86856823266219 24.748322147651006 22 30.019574944071586 25.083892617449667 21.742170022371365 23.154362416107382 23 26.25838926174497 25.852908277404925 24.482662192393736 23.406040268456376 24 23.461968680089484 28.048098434004476 26.482102908277405 22.007829977628635 25 26.24440715883669 25.978747203579417 27.46085011185682 20.315995525727068 26 21.706293706293707 28.195804195804197 29.118881118881117 20.97902097902098 27 23.163051084674596 22.141357592722184 33.78586424072778 20.909727081875438 28 21.884626155138616 23.942873144777373 34.780173620834496 19.39232707924951 29 18.044481745698697 25.346202266051193 36.5925304238355 20.016785564414604 30 17.829674171444555 23.968675709690952 41.7284295902671 16.4732205285974 31 16.1001538676738 25.192334592250663 43.01300881242132 15.694502727654216 32 18.73775538762944 21.43856703050658 43.99664147774979 15.827036104114189 33 17.92968202829528 21.795769715646447 44.684129429892145 15.59041882616613 34 13.993842709207948 23.831514133781138 47.76098516652672 14.413657990484188 35 14.437604924454392 22.817571348628988 49.72020145495243 13.024622271964187 36 16.41728429590267 21.84309886729129 49.13998042231856 12.599636414487483 37 15.57422969187675 21.5406162464986 48.99159663865546 13.893557422969188 38 15.425606506801289 21.86229140373019 49.90884868882345 12.80325340064507 39 13.701316895488933 22.41524236480807 48.585037825721486 15.298402913981507 40 10.72080932977378 19.15132780666011 43.83869607980891 26.2891667837572 41 10.180230920867361 17.136018023092088 40.706843142776684 31.97690791326387 42 14.115670074219297 22.39182187368716 50.07702002520655 13.41548802688699 43 10.974071478626488 20.71478626489138 50.091100210231254 18.220042046250875 44 12.403427447675236 21.351313386711617 48.46186262115466 17.78339654445849 45 13.479052823315119 22.78268179907524 50.1891551071879 13.549110270421746 46 14.125560538116591 24.635650224215247 49.509529147982065 11.729260089686099 47 13.594405594405595 23.734265734265733 50.55944055944056 12.111888111888112 48 12.340663958537611 24.61129009665219 52.374282112340666 10.673763832469534 49 10.040724617329026 21.387445583485466 46.440106726583345 22.131723072602163 50 11.668300882476537 25.24163048045945 51.141616472895365 11.94845216416865 51 13.560510447342589 25.83087925957089 50.31552376945731 10.293086523629224 52 13.157894736842104 26.049832026875702 49.21612541993281 11.576147816349385 53 13.135415207954068 27.909256406665733 48.55062316202213 10.404705223358073 54 11.988222097588334 26.500280426247897 45.55524397083567 15.9562535053281 55 13.69114349775785 26.97589686098655 49.55156950672646 9.781390134529149 56 14.06359434094411 26.99257599103516 46.5331278890601 12.41070177896064 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 2.5 14 4.0 15 10.0 16 16.0 17 27.0 18 38.0 19 57.0 20 76.0 21 83.5 22 94.0 23 97.0 24 122.0 25 147.0 26 175.5 27 204.0 28 249.5 29 342.5 30 390.0 31 426.0 32 462.0 33 478.5 34 495.0 35 476.0 36 457.0 37 378.0 38 271.5 39 244.0 40 192.0 41 140.0 42 139.5 43 139.0 44 135.0 45 131.0 46 138.5 47 140.5 48 135.0 49 164.5 50 194.0 51 218.0 52 242.0 53 243.5 54 213.5 55 182.0 56 196.0 57 210.0 58 212.0 59 214.0 60 210.5 61 207.0 62 213.0 63 246.0 64 273.0 65 252.0 66 231.0 67 225.0 68 219.0 69 200.0 70 181.0 71 164.0 72 138.0 73 129.0 74 125.0 75 121.0 76 93.0 77 65.0 78 48.5 79 27.0 80 22.0 81 13.5 82 5.0 83 5.5 84 6.0 85 3.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.013982102908277404 14 0.0 15 0.2656599552572707 16 0.05592841163310962 17 0.0 18 0.0 19 0.08389261744966443 20 0.09787472035794184 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.02796420581655481 27 0.09787472035794184 28 0.13982102908277405 29 0.04194630872483222 30 0.013982102908277404 31 0.04194630872483222 32 0.08389261744966443 33 0.18176733780760626 34 0.08389261744966443 35 0.05592841163310962 36 0.013982102908277404 37 0.16778523489932887 38 0.2936241610738255 39 0.19574944071588368 40 0.4893736017897092 41 0.6991051454138703 42 0.15380313199105144 43 0.23769574944071586 44 0.4614093959731544 45 0.20973154362416108 46 0.22371364653243847 47 0.02796420581655481 48 0.18176733780760626 49 0.4334451901565996 50 0.18176733780760626 51 0.2936241610738255 52 0.11185682326621924 53 0.15380313199105144 54 0.2796420581655481 55 0.22371364653243847 56 0.18176733780760626 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 7152.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.46700223713647 #Duplication Level Percentage of deduplicated Percentage of total 1 87.88879235447436 70.72147651006712 2 7.437011294526499 11.968680089485458 3 2.363162467419635 5.704697986577181 4 0.7471763683753259 2.4049217002237135 5 0.5039096437880104 2.027404921700224 6 0.33014769765421376 1.5939597315436242 7 0.2258905299739357 1.2723713646532437 8 0.26064291920069504 1.6778523489932886 9 0.06950477845351868 0.5033557046979865 >10 0.1737619461337967 2.1252796420581657 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCTGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23 0.3215883668903803 No Hit GGCTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 18 0.25167785234899326 No Hit GGCTGCGGGTAACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.23769574944071586 No Hit GGCTGCGGGATGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.22371364653243847 No Hit GGCTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 16 0.22371364653243847 No Hit GGCTGCGGGAAGCATGTACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 15 0.20973154362416108 No Hit GGCTGCGGGAGCATGGTCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.16778523489932887 No Hit GGCTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA 12 0.16778523489932887 No Hit GGCTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 12 0.16778523489932887 No Hit GGCTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 11 0.15380313199105144 No Hit GGCTGCGGGAAGATGGACACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.12583892617449663 No Hit GGCTGCGGGGCTGGCTGCGGGAAGCATGGTATCAACGCAGAGTTTTTTTTTTTTTT 9 0.12583892617449663 No Hit GGCTGCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG 9 0.12583892617449663 No Hit GGCTGCGGGAGAGCACCAGGAGCTCAGGGTCCGGTTGGGCAGCTGAGTGCCATTCG 9 0.12583892617449663 No Hit GGCTGCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGAG 8 0.11185682326621924 No Hit GGCTGCGGGAAAGTGTCAACGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.11185682326621924 No Hit GGCTGCGGGATGGAACGCAGAGTCGACTTTTTTTTTTTTTCTTTTTTTTTTTTTTT 8 0.11185682326621924 No Hit GGCTGCGGGAACATTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.11185682326621924 No Hit GGCTGCGGGACTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.11185682326621924 No Hit GGCTGCGGGGTTTTCAAGGCCTGTTAAAATTCTGTTGTATATAGTAGTTTTTGAGC 8 0.11185682326621924 No Hit GGCTGCGGGAAGAGTACACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.11185682326621924 No Hit GGCTGCGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTC 8 0.11185682326621924 No Hit GGCTGCGGGGCTGGCTGCGGGAAGCATGGTATCAACGCAGCGTTTTTTTTTTTTTT 8 0.11185682326621924 No Hit GGCTGCGGGACGCAGAGTCGACTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTT 8 0.11185682326621924 No Hit GGCTGCGGGTACGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.11185682326621924 No Hit GGCTGCGGGAAGAGTGGTATCAACCAGAATCGACTTTTTTTTTTTTTTTTTTTTTT 8 0.11185682326621924 No Hit GGCTGCGGGAGATCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.11185682326621924 No Hit GGCTGCGGGAGAGTGGAAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.11185682326621924 No Hit GGCTGCGGCCCAGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.11185682326621924 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.013982102908277404 0.0 0.0 0.0 0.0 11 0.013982102908277404 0.0 0.0 0.0 0.0 12 0.013982102908277404 0.0 0.0 0.0 0.0 13 0.013982102908277404 0.0 0.0 0.0 0.0 14 0.013982102908277404 0.0 0.0 0.0 0.0 15 0.013982102908277404 0.0 0.0 0.0 0.0 16 0.013982102908277404 0.0 0.0 0.0 0.0 17 0.013982102908277404 0.0 0.0 0.0 0.0 18 0.02796420581655481 0.0 0.0 0.0 0.0 19 0.02796420581655481 0.0 0.0 0.0 0.0 20 0.02796420581655481 0.0 0.0 0.0 0.0 21 0.02796420581655481 0.0 0.0 0.0 0.0 22 0.02796420581655481 0.0 0.0 0.0 0.0 23 0.02796420581655481 0.0 0.0 0.0 0.0 24 0.02796420581655481 0.0 0.0 0.0 0.0 25 0.02796420581655481 0.0 0.0 0.0 0.0 26 0.02796420581655481 0.0 0.0 0.0 0.0 27 0.05592841163310962 0.0 0.0 0.0 0.0 28 0.06991051454138703 0.0 0.0 0.0 0.0 29 0.06991051454138703 0.0 0.0 0.0 0.0 30 0.06991051454138703 0.0 0.0 0.0 0.0 31 0.06991051454138703 0.0 0.0 0.0 0.0 32 0.08389261744966443 0.0 0.0 0.0 0.0 33 0.08389261744966443 0.0 0.0 0.0 0.0 34 0.08389261744966443 0.0 0.0 0.0 0.0 35 0.09787472035794184 0.0 0.0 0.0 0.0 36 0.09787472035794184 0.0 0.0 0.0 0.0 37 0.11185682326621924 0.0 0.0 0.0 0.0 38 0.11185682326621924 0.0 0.0 0.0 0.0 39 0.11185682326621924 0.0 0.0 0.0 0.0 40 0.11185682326621924 0.0 0.0 0.0 0.0 41 0.11185682326621924 0.0 0.0 0.0 0.0 42 0.11185682326621924 0.0 0.0 0.0 0.0 43 0.11185682326621924 0.0 0.0 0.0 0.0 44 0.11185682326621924 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTTGT 15 0.007932022 50.25352 32 GCGGGGG 75 0.0 49.9021 5 GCGGGCT 45 1.0186341E-10 49.9021 5 GCGGGCA 15 0.008157543 49.9021 5 GCGGGAT 60 0.0 49.9021 5 GCGGGAG 85 0.0 49.9021 5 ACAAAAC 15 0.008157543 49.9021 48 GAGTGGT 15 0.008157543 49.9021 9 CGGGGTG 15 0.008157543 49.9021 6 GGGTATC 15 0.008157543 49.9021 7 CGGGGGT 15 0.008157543 49.9021 6 CGGGGGG 25 1.9069104E-5 49.9021 6 CGGGGGC 25 1.9069104E-5 49.9021 6 CGGGGAC 15 0.008157543 49.9021 6 CGGGCTG 35 4.436697E-8 49.9021 6 CGGGTAT 15 0.008157543 49.9021 6 CGGGATG 25 1.9069104E-5 49.9021 6 CGGGACT 15 0.008157543 49.9021 6 GGGAAGA 25 1.9069104E-5 49.9021 7 GGGGAGT 15 0.008157543 49.9021 7 >>END_MODULE