FastQCFastQC Report
Fri 17 Jun 2016
SRR1526232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180399
Sequences flagged as poor quality0
Sequence length56
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG41952.3254009168565237No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG11320.6274979351326782No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG11310.6269436083348577No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG9130.5061003664100134No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5340.2960105100360867No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3510.19456870603495585No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2960.16408073215483457No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2940.1629720785591938No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG2820.15632015698534915No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG2440.13525573866817445No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG2260.12527785630740748No Hit
TAGCATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC2120.11751728113792205No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1870.10365911119241238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG150.00815475750.40561741
CGTCACG204.0167323E-450.39146437
ATGCTCG1650.050.306736
TATGCTC1700.050.27850735
TATGCCT850.050.27850735
TTGTCGA150.00826398850.23628234
CGATTGT150.00826398850.23628244
ATATGCC1350.050.23628234
ATATGCA150.00826398850.23628234
GTTAAGC204.078359E-450.23628234
CGATTCA950.050.19412649
CGATTAA1850.050.19412649
ACTGCTA150.00830064150.18008845
TCGTGCG150.00830064150.18008846
CTACCAA252.0365702E-550.18008846
CGTTCGT252.0433212E-550.15204242
GTCAGCT150.00831901150.1520442
ATGATTT301.0331478E-650.0680833
CGCAGTA150.0084670749.9287713
CTTTCTA150.0084670749.9287713
Produced by FastQC (version 0.11.5)