##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526232_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	180399
Sequences flagged as poor quality	0
Sequence length	56
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.648617786129634	39.0	38.0	39.0	33.0	40.0
2	36.138432031219686	39.0	36.0	39.0	29.0	40.0
3	37.1020848230866	39.0	38.0	39.0	33.0	40.0
4	37.0743629399276	39.0	37.0	39.0	33.0	40.0
5	35.46402696245545	39.0	36.0	39.0	22.0	40.0
6	37.19146447596716	39.0	38.0	39.0	33.0	40.0
7	37.56872266475978	39.0	38.0	39.0	34.0	40.0
8	37.40348893286548	39.0	38.0	39.0	33.0	40.0
9	37.35904855348422	39.0	38.0	39.0	33.0	40.0
10	36.890553717038344	39.0	37.0	39.0	33.0	40.0
11	36.39109972893419	38.0	35.0	39.0	31.0	40.0
12	34.67872881778724	38.0	34.0	39.0	24.0	40.0
13	34.25429187523213	38.0	33.0	39.0	23.0	40.0
14	34.28298937355529	38.0	33.0	39.0	23.0	40.0
15	34.2775680574726	38.0	33.0	39.0	23.0	40.0
16	33.79447779644011	38.0	33.0	39.0	22.0	40.0
17	33.7303920753441	37.0	32.0	39.0	22.0	40.0
18	33.20245677636794	37.0	31.0	39.0	20.0	40.0
19	33.954561832382666	37.0	32.0	39.0	25.0	40.0
20	33.98875270927223	37.0	32.0	39.0	25.0	40.0
21	34.74124579404542	38.0	33.0	39.0	26.0	40.0
22	34.491382989927885	38.0	33.0	39.0	25.0	40.0
23	35.18337684798696	38.0	34.0	39.0	27.0	40.0
24	34.74979905653579	38.0	33.0	39.0	26.0	40.0
25	35.03687381859101	38.0	33.0	39.0	27.0	40.0
26	35.65206569881208	38.0	35.0	39.0	29.0	40.0
27	35.55596760514194	38.0	35.0	39.0	29.0	40.0
28	35.34703629177545	38.0	35.0	39.0	28.0	40.0
29	35.26064446033514	38.0	35.0	39.0	28.0	40.0
30	35.01667415007844	38.0	35.0	39.0	27.0	40.0
31	35.312063814100966	38.0	35.0	39.0	29.0	40.0
32	35.0588750491965	38.0	35.0	39.0	27.0	40.0
33	35.23457447103365	38.0	35.0	39.0	29.0	40.0
34	35.00276054745314	38.0	35.0	39.0	28.0	40.0
35	34.89438411521128	38.0	35.0	39.0	27.0	40.0
36	34.44871645629965	38.0	33.0	38.0	28.0	39.0
37	34.23374298083692	37.0	33.0	38.0	27.0	39.0
38	33.97760519736806	37.0	33.0	38.0	26.0	39.0
39	33.35821706328749	36.0	32.0	38.0	23.0	39.0
40	30.220073282002673	35.0	26.0	38.0	10.0	39.0
41	27.028259580152884	32.0	16.0	38.0	6.0	39.0
42	31.04715658069058	35.0	29.0	38.0	13.0	39.0
43	29.571422236265168	34.0	26.0	38.0	7.0	39.0
44	29.58616178581921	34.0	27.0	38.0	5.0	39.0
45	30.63178288127983	35.0	29.0	38.0	4.0	39.0
46	30.12140311199064	35.0	27.0	38.0	4.0	39.0
47	29.661239807316004	35.0	27.0	38.0	4.0	39.0
48	29.680347452036873	35.0	27.0	38.0	4.0	39.0
49	27.254108947388843	33.0	17.0	38.0	4.0	39.0
50	29.233853846196485	35.0	26.0	38.0	4.0	39.0
51	29.48046275201082	35.0	27.0	39.0	4.0	40.0
52	29.25097145771318	35.0	26.0	39.0	4.0	40.0
53	28.758030809483422	35.0	24.0	38.0	4.0	40.0
54	27.813912493971696	34.0	19.0	38.0	4.0	39.0
55	28.111641417081024	34.0	23.0	38.0	4.0	39.0
56	26.410844849472557	33.0	17.0	38.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.6663035354321494
1	2	2.5200470714566734
1	3	1.210153544068504
1	4	1.0226878649311928
1	5	3.324427401798829
1	6	1.0527318771689451
1	7	0.8432681705339888
1	8	0.8170704781203213
1	9	0.7126039813047598
1	10	1.000436637564782
1	11	-2.741813269476225
1	12	-13.660441150251916
1	13	-8.764396757449319
1	14	-5.976942280518017
1	15	-6.272286338819654
1	16	-22.853185083228094
1	17	-4.195272981062143
1	18	-12.043067328285385
1	19	-5.344594596471794
1	20	-4.891359669869846
1	21	-6.674764686724778
1	22	-2.3549675072269203
1	23	-3.56417831364751
1	24	-3.492656118150123
1	25	-3.3782271012873437
1	26	1.190909526407495
1	27	1.3770318111646773
1	28	1.6345335573352244
1	29	1.6097318920556347
1	30	1.4204056617621532
1	31	1.1789586642258598
1	32	1.1603960237761157
1	33	0.7410563312004399
1	34	0.7773241227943117
1	35	0.5342255118503658
1	36	1.3505670304156112
1	37	1.6708530576640968
1	38	1.3936860704814293
1	39	2.1162524400325537
1	40	4.586561876053668
1	41	-8.98895414982298
1	42	-8.76663492880019
1	43	-16.735720945125454
1	44	-22.35621177680704
1	45	-10.198404180862354
1	46	-17.079791180995098
1	47	-23.04134537994619
1	48	-14.09815549752256
1	49	-19.812148780499932
1	50	-12.961825293707706
1	51	-9.898992370821386
1	52	-9.877670754902404
1	53	-9.82129469152618
1	54	-8.767582669466815
1	55	-8.940684643688869
1	56	-3.974125426855725
2	1	1.576753025700917
2	2	3.250217754187595
2	3	2.0922009645195416
2	4	1.7974793385178458
2	5	4.173882916163983
2	6	1.7516235978980177
2	7	1.528875986412892
2	8	1.84765435207769
2	9	1.7387663229791528
2	10	1.7378864367615705
2	11	0.980634212760414
2	12	-2.5561200195753635
2	13	-1.6665438071867342
2	14	2.756221142404442
2	15	2.6180442141615003
2	16	2.2967801623696786
2	17	3.1540473772943614
2	18	-1.4367381654803175
2	19	2.331397834791723
2	20	2.4739477135098227
2	21	2.796659471446368
2	22	2.977535582053278
2	23	2.183369570195559
2	24	1.6378080461995808
2	25	2.122081826732426
2	26	2.082147555446724
2	27	2.286206973351888
2	28	2.1077147436188213
2	29	2.2640800539339168
2	30	2.034458025061504
2	31	1.7814841507874917
2	32	1.8569128603531908
2	33	1.7543209280493741
2	34	2.058410004783809
2	35	1.8506450361012128
2	36	2.272466165417157
2	37	2.2917211453996487
2	38	2.358371736221315
2	39	2.871137364345195
2	40	5.338666448188366
2	41	-6.391564591590047
2	42	-5.08734082932537
2	43	-13.122633981887889
2	44	-13.509092530591408
2	45	-4.716399238014038
2	46	-9.528740825727876
2	47	-17.03881603178431
2	48	-6.758624295792565
2	49	-11.998451684423316
2	50	-6.303634839583516
2	51	-1.057009052934454
2	52	-0.6586987128881923
2	53	-0.5648458191134509
2	54	0.340445597446994
2	55	0.3411156343463517
2	56	3.6889885675835714
3	1	1.3028419969706135
3	2	3.1479316868412894
3	3	1.897653544068504
3	4	1.7919186341619593
3	5	4.15038894026037
3	6	1.707539569476637
3	7	1.5519220166878398
3	8	1.609378170428009
3	9	1.4270270582278357
3	10	1.4908212529493952
3	11	1.6937636536006977
3	12	3.6309050035942363
3	13	3.1779109348583745
3	14	3.4538269502512122
3	15	2.9546367381034244
3	16	4.145853378310363
3	17	4.070111634322473
3	18	4.813663440945383
3	19	3.465982326605129
3	20	3.600948022437848
3	21	2.836773774813686
3	22	3.3229171081576965
3	23	2.656013994044798
3	24	3.0775361895421867
3	25	2.533311360251119
3	26	2.1091787571767213
3	27	2.023185657318521
3	28	2.14511048041215
3	29	1.9616549689787135
3	30	1.8655979694544627
3	31	1.7808817411489386
3	32	1.7392421776222662
3	33	1.8170178696619743
3	34	1.3830933535635381
3	35	1.5111485887734375
3	36	2.5303747227233018
3	37	2.351622288433326
3	38	2.7888783781737416
3	39	3.3518293631094807
3	40	6.799061876053674
3	41	7.2764304655616385
3	42	3.9160573788921127
3	43	-1.4116824835869934
3	44	1.7649420693468052
3	45	3.8592881268299486
3	46	2.581747280543361
3	47	-2.6807684568692665
3	48	2.9874214255543627
3	49	4.852274296423147
3	50	3.4660593216769087
3	51	4.466392244563231
3	52	4.307906168174515
3	53	4.453705308473822
3	54	5.092994253610108
3	55	4.458353817849595
3	56	4.22202841929812
4	1	1.56389205802067
4	2	3.1420708809804836
4	3	1.8430137394286987
4	4	1.8374009296442537
4	5	4.256738146609578
4	6	1.6991757111127797
4	7	1.8676118823777017
4	8	1.5890485000983432
4	9	1.5338036150043948
4	10	1.5103572724854146
4	11	1.6289895388265876
4	12	3.5939086665979048
4	13	3.9956765026239367
4	14	3.411030857455117
4	15	4.006407189873876
4	16	3.958857041314033
4	17	4.098683062893905
4	18	4.842234869516815
4	19	3.2177527783755835
4	20	3.514012735502561
4	21	2.850876338916251
4	22	3.1324409176815067
4	23	2.481227669258473
4	24	3.0631283751343688
4	25	2.9221391990789556
4	26	1.3588735068714755
4	27	1.7637839479168136
4	28	1.776246011547677
4	29	1.7298478871716298
4	30	2.0132780671345643
4	31	1.3173896776568768
4	32	1.6257501141302058
4	33	1.264026416670525
4	34	1.094937065407251
4	35	1.2201229477477966
4	36	2.0282990206476015
4	37	1.9351998220108584
4	38	2.465801455096816
4	39	3.5413898026699187
4	40	6.526656503648301
4	41	8.467395056526229
4	42	4.965202678037414
4	43	6.384776612872102
4	44	6.013904218308955
4	45	4.42430644184827
4	46	4.6906605894566695
4	47	3.9129433868425743
4	48	4.885284673417612
4	49	6.4797468238956775
4	50	4.198660054277642
4	51	3.6977719759429633
4	52	3.5465508568192057
4	53	3.8198835746520885
4	54	4.412591323207177
4	55	3.2629936224894003
4	56	4.083566880836582
5	1	1.413438543281444
5	2	3.359077683701571
5	3	2.0774808596101053
5	4	1.6719814285104704
5	5	4.282021593321595
5	6	1.7255929446728757
5	7	1.7133034923550312
5	8	1.883946459282015
5	9	1.862034907521405
5	10	1.654008066136214
5	11	1.843842146536339
5	12	3.8830243128564064
5	13	4.027989427794012
5	14	4.055475301899563
5	15	4.377949139987251
5	16	4.339809422266413
5	17	4.741540205751043
5	18	5.002438951149465
5	19	3.82602942236651
5	20	4.1536952751851
5	21	3.066522597419649
5	22	2.7147538428515716
5	23	2.851068939099747
5	24	3.100770098490379
5	25	3.2765609677864447
5	26	2.2591002642410842
5	27	2.4815844014315545
5	28	2.3092392088265896
5	29	3.066129066309948
5	30	3.049899382327304
5	31	1.8737388840060802
5	32	2.059650340887572
5	33	1.5505343531784632
5	34	1.8640980631396786
5	35	1.528966485162762
5	36	2.654943011577302
5	37	2.8092361031899955
5	38	2.968862679586614
5	39	3.534246945527066
5	40	7.052280086414736
5	41	9.716147890993348
5	42	6.107266170100907
5	43	7.4549580187677975
5	44	6.696103764794213
5	45	6.048456101712215
5	46	5.954039274263927
5	47	5.82190030294235
5	48	5.676667893372258
5	49	7.9919917218548555
5	50	6.154442367202808
5	51	5.110583767326183
5	52	4.915598475866826
5	53	5.337457270797209
5	54	5.735380438853433
5	55	4.834648951287583
5	56	5.074383207367195
6	1	1.6574961663306667
6	2	3.1308663991877665
6	3	1.7753199952643683
6	4	1.8119574189066299
6	5	4.186943562109107
6	6	1.6443204356692718
6	7	1.5331114155242958
6	8	1.54072917236725
6	9	1.5120949315310028
6	10	1.2935038644555377
6	11	1.7940222185651464
6	12	3.5168778542729697
6	13	3.772287146881645
6	14	3.977324041395363
6	15	4.262943137586291
6	16	4.331406060921871
6	17	4.2751536511291945
6	18	4.3611424325420245
6	19	3.823028221886318
6	20	3.6209221659414013
6	21	2.769643845919049
6	22	3.184261645972825
6	23	2.528379863469489
6	24	3.199449570279093
6	25	2.7442680506195742
6	26	1.9979958224643752
6	27	2.287826898430353
6	28	1.8651815857773713
6	29	1.9642082980026316
6	30	2.592876573203654
6	31	1.6140750184598645
6	32	2.262771589386972
6	33	1.5975931767078748
6	34	1.6701004641000665
6	35	1.868582331501301
6	36	2.049540850712958
6	37	2.272501409312447
6	38	1.917482127365723
6	39	3.1107175337623545
6	40	6.99117564463803
6	41	3.2400374468156734
6	42	5.081215749932838
6	43	6.40741900316155
6	44	5.389020931660962
6	45	4.268143976862273
6	46	5.4219864531354744
6	47	5.149871491417738
6	48	4.6742669329881075
6	49	6.992351865912479
6	50	5.330312715342064
6	51	4.556081966605895
6	52	4.533245534690355
6	53	4.956905049908855
6	54	5.194804208361237
6	55	4.5617798036285215
6	56	5.673903015290364
7	1	1.682085748999178
7	2	3.060395172722494
7	3	2.0043559134291016
7	4	1.7711461576932805
7	5	4.125283455028303
7	6	1.8987437239719327
7	7	1.8518895596933547
7	8	2.015058144462415
7	9	1.6822364058422465
7	10	1.8195294645690012
7	11	2.020540674048604
7	12	3.6285680999915115
7	13	3.7321141156438316
7	14	4.34251568767413
7	15	4.4557240474185775
7	16	4.3373983312477264
7	17	4.663782520398421
7	18	5.043410276388421
7	19	3.8147489608148035
7	20	3.987088885793895
7	21	2.895180136384603
7	22	3.310258880309597
7	23	2.735095461100947
7	24	3.3773739045697724
7	25	3.0062259983556316
7	26	1.9389418211170053
7	27	1.8457108114892478
7	28	1.905691460613383
7	29	2.308409269530479
7	30	2.4114597026579716
7	31	1.5992361715286876
7	32	1.662870869605392
7	33	1.4936326051374706
7	34	1.3503117891364056
7	35	1.494384551123325
7	36	2.146392249500323
7	37	2.445195803525827
7	38	2.5048309909617927
7	39	3.0162240401623848
7	40	5.9821517819283905
7	41	0.08594036494495327
7	42	4.090026869317306
7	43	5.58846357288818
7	44	5.304490917123498
7	45	3.8497133134576735
7	46	4.592961172137002
7	47	5.04399622992074
7	48	4.848716459634208
7	49	7.166003605089163
7	50	5.079009662475354
7	51	4.606200748295784
7	52	4.396611134094673
7	53	4.621621569428058
7	54	5.413344789150514
7	55	4.183929929501655
7	56	4.057195572819705
8	1	1.567542851671469
8	2	3.1881026270122277
8	3	1.5782518346667942
8	4	1.6264485486918758
8	5	4.124833384704814
8	6	1.7504455523826223
8	7	1.5160245807904005
8	8	1.2536516747015156
8	9	1.4463432975440753
8	10	1.45210330423145
8	11	1.0824816023186514
8	12	3.539622952312186
8	13	3.3599622169096577
8	14	3.8046816511059163
8	15	3.5783119517786375
8	16	3.7512379936949856
8	17	4.116778300989139
8	18	4.530330107612052
8	19	3.509657540280344
8	20	3.3816317831216054
8	21	2.661987450027361
8	22	2.478472663713255
8	23	2.247894335925139
8	24	2.929160121166113
8	25	1.7357899927297495
8	26	1.642683030680999
8	27	2.358228392361262
8	28	2.0065634718651424
8	29	1.8463558236795663
8	30	1.9620082258647216
8	31	1.2819928522600534
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115	20	-3.016081768580385
115	21	2.254537225236092
115	22	2.3460384954409363
115	23	1.9645288895539181
115	24	2.360862112174466
115	25	1.5388471539372048
115	26	1.6065109047978865
115	27	2.057778809830751
115	28	1.6747458739203722
115	29	1.718057814687917
115	30	0.9990795168088411
115	31	0.726257463692292
115	32	0.4219011371598427
115	33	0.4790232053667367
115	34	1.0149297252843041
115	35	0.7037452984221702
115	36	1.3789963323275742
115	37	1.4306351830531554
115	38	2.0395008770621317
115	39	2.3958489273602552
115	40	6.054686598152383
115	41	5.096305965783955
115	42	0.3398012650637483
115	43	-7.349855004707489
115	44	-7.028232674984007
115	45	0.673205423406209
115	46	-4.7406404473356965
115	47	-3.8270556956420556
115	48	-4.144259321444302
115	49	-14.267156561736035
115	50	-3.6715573968646638
115	51	-2.4665779644185406
115	52	-2.0265980559250814
115	53	-4.1878464923265355
115	54	-6.836307658350766
115	55	-2.2635225716568534
115	56	-0.20689554690908452
116	1	0.9573160943018522
116	2	2.7713225816607547
116	3	1.549929839201944
116	4	1.3974916325921
116	5	3.749368532097101
116	6	0.9939602916116499
116	7	1.0643239005182963
116	8	1.1961913572411973
116	9	1.1477491932356898
116	10	1.4264570457280499
116	11	-1.6051374453004001
116	12	-13.290445074898699
116	13	-8.043439143634565
116	14	-3.037381840957579
116	15	-6.273071268176011
116	16	-3.803047720590733
116	17	-2.4166230595550786
116	18	-8.933275334564819
116	19	-3.005603230694714
116	20	-3.0330394186924536
116	21	2.078767495378834
116	22	1.776998740810761
116	23	1.7204566942017863
116	24	1.766076220939361
116	25	1.5837038249293016
116	26	2.0244063866900674
116	27	1.6050537891866554
116	28	1.2439330863776092
116	29	2.0671494744732186
116	30	1.777450402735468
116	31	1.5441470472713874
116	32	1.6943442184385944
116	33	0.6954323123621364
116	34	0.9855266345682523
116	35	1.3187624035301084
116	36	2.1467797462711857
116	37	2.2214810011491792
116	38	1.9402912510151822
116	39	2.7505734761393086
116	40	6.420647433353512
116	41	4.73043360527906
116	42	-0.7672236258174614
116	43	-9.709327695517917
116	44	-7.39879419438946
116	45	-1.2474622656347272
116	46	-3.8541239910421936
116	47	-2.8546303093025465
116	48	-4.012107616831823
116	49	-7.040661339369631
116	50	-3.8659657960624934
116	51	-2.1230897020615735
116	52	-1.7017484629086859
116	53	-3.5707059945089092
116	54	-7.675844050942484
116	55	-1.7908612527940484
116	56	-1.452147404877703
117	1	0.19277877252382325
117	2	1.9421361125525607
117	3	0.8020265683045409
117	4	0.758198929209378
117	5	3.0195670224521507
117	6	0.5720285051984533
117	7	0.318307685813231
117	8	0.432981963894818
117	9	0.3597983964786309
117	10	0.8857106101675214
117	11	-2.3223585803297553
117	12	-7.474258326226625
117	13	-10.693005819620026
117	14	-10.100493386945836
117	15	-8.200105095405533
117	16	-14.054515430962553
117	17	-12.220299324581635
117	18	-14.391131079602559
117	19	-5.276430739780537
117	20	-5.770179480196507
117	21	0.48329643024045055
117	22	0.6105883410344077
117	23	-0.18924417244624436
117	24	0.010834398182858251
117	25	0.41365909470844286
117	26	1.6170818130249813
117	27	1.514544982924427
117	28	1.8122864551223685
117	29	1.4502218815182246
117	30	1.9037433856820698
117	31	0.9117036589571583
117	32	1.5145846433756915
117	33	1.297892474509183
117	34	1.3778246496647029
117	35	0.950716555053738
117	36	2.061512762765453
117	37	1.775436830056087
117	38	2.434979537288598
117	39	3.1152645580515212
117	40	6.485995490384543
117	41	5.43912804195784
117	42	0.30804894896587953
117	43	-9.50014402204853
117	44	-8.967776581864996
117	45	0.03895092978042669
117	46	-2.140117840636826
117	47	-2.6138559173539875
117	48	-2.5401338958365756
117	49	-7.430233606632701
117	50	-3.076617179903703
117	51	-1.9659049103366648
117	52	-2.974812483037283
117	53	-4.368581309018275
117	54	-9.407532616779775
117	55	-3.175471261180963
117	56	-1.871912571218214
118	1	0.47360069033204866
118	2	2.407889537210096
118	3	1.0896978011812521
118	4	1.0321715319491034
118	5	3.300388940260369
118	6	0.935042203828587
118	7	0.6470748091009071
118	8	0.8165436077304378
118	9	0.7296614101772576
118	10	0.9884503361949157
118	11	-1.877153100877699
118	12	-8.097545997459502
118	13	-8.295745545647424
118	14	-13.621041332151314
118	15	-6.01517358855622
118	16	-12.958625020003648
118	17	-12.829888365677526
118	18	-14.185651627547767
118	19	-5.824375945259991
118	20	-5.770179480196507
118	21	0.7230224576377111
118	22	1.3777116287056401
118	23	0.8587010330332063
118	24	1.0724782337992949
118	25	0.9136590947084429
118	26	1.7129722239838898
118	27	1.6104353938833285
118	28	1.7163960441634671
118	29	1.6283040732990486
118	30	2.191414618558781
118	31	0.966498179505102
118	32	-3.5196619319667732
118	33	1.2499472690297324
118	34	1.1380986222674423
118	35	0.943867239985245
118	36	2.088910023039425
118	37	2.302834090330059
118	38	2.6541576194803795
118	39	3.1015659279145353
118	40	6.485995490384543
118	41	5.1925526994920865
118	42	-0.5481154345957648
118	43	-9.383705665884147
118	44	-6.563666992823901
118	45	-1.091186056520943
118	46	-9.153816470773812
118	47	-10.75084221872385
118	48	-10.57438047117904
118	49	-9.82064456553681
118	50	-7.336891152506443
118	51	-5.493302170610637
118	52	-5.550154948790709
118	53	-6.772690898059373
118	54	-10.921231246916763
118	55	-4.702868521454938
118	56	-2.5157481876565697
119	1	0.5502619683036301
119	2	2.2921744006492233
119	3	0.9596458995391188
119	4	0.9873319165317653
119	5	3.3904510520616142
119	6	0.86774023837296
119	7	0.6721322412458903
119	8	0.8017814193529986
119	9	0.7786276315675451
119	10	1.184442848744908
119	11	-3.4250132216979168
119	12	-9.61830665431307
119	13	-10.334033642303595
119	14	-11.902288673255715
119	15	-9.30812007030556
119	16	-14.393109832391975
119	17	-15.997590229031562
119	18	-16.112734074582566
119	19	-6.401122307890809
119	20	-7.440093544690679
119	21	0.06723244657670335
119	22	0.6583208348657692
119	23	0.6361621068016063
119	24	0.7394568775498271
119	25	0.36209779121146113
119	26	1.6087009740902474
119	27	1.5972898140313774
119	28	0.9972605043013019
119	29	1.6370252508707352
119	30	1.7253208552643073
119	31	0.5594531697203706
119	32	-4.945850990079595
119	33	0.6337641668430578
119	34	0.22399158576612166
119	35	0.714946964310947
119	36	1.4994860461824544
119	37	2.0209486142813873
119	38	1.3803977283887434
119	39	2.164060610123336
119	40	6.185731727940915
119	41	3.1026607570093176
119	42	-1.298945010023317
119	43	-10.344864518942941
119	44	-7.068048852774552
119	45	-4.147639727923991
119	46	-13.378525055815931
119	47	-11.180051782239548
119	48	-11.751016223752853
119	49	-9.392923983557743
119	50	-9.309621519221324
119	51	-7.272379852904521
119	52	-6.525395312953048
119	53	-8.736189579247153
119	54	-9.735959401430506
119	55	-6.664197543832202
119	56	-4.309290262020561
120	1	-0.015062512313207321
120	2	1.7290987955946022
120	3	0.4851867005671764
120	4	0.6221573609524143
120	5	2.748664802329337
120	6	0.2597942113864562
120	7	0.180967109526037
120	8	0.20234899270918305
120	9	0.3092551749387127
120	10	0.13405732721995633
120	11	-3.21843793791124
120	12	-11.298537967228043
120	13	-10.3360147945846
120	14	-12.779992678396003
120	15	-12.29682214783822
120	16	-18.045658558029157
120	17	-15.459198710505113
120	18	-15.844957248709786
120	19	-8.050993800715823
120	20	-14.10886630116958
120	21	-5.03418113234812
120	22	-3.823634615980236
120	23	-3.7649409131170053
120	24	-8.839307311784076
120	25	-3.40011039041201
120	26	-0.04930930648375664
120	27	-0.0168673930793517
120	28	0.16089297378084666
120	29	0.035129770039716846
120	30	-0.21304924539964532
120	31	-0.920842396782092
120	32	-11.871102649963937
120	33	-0.9189237749003567
120	34	-0.39343184537545284
120	35	-0.679302339608526
120	36	-0.47320617913346297
120	37	-0.37158726063829306
120	38	-0.2755778552480095
120	39	0.022916896265975595
120	40	2.810865589581521
120	41	-2.290678287754016
120	42	-6.360467820046875
120	43	-15.689799194462324
120	44	-13.249779416064335
120	45	-13.115512934178007
120	46	-17.357642639881043
120	47	-16.68010532689579
120	48	-14.221995020068979
120	49	-18.45621376723733
120	50	-14.024656858694444
120	51	-12.546207224932791
120	52	-12.593022213788347
120	53	-13.322289386486391
120	54	-13.711382404214827
120	55	-11.62917933864908
120	56	-8.99401932605997
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	760.0
5	30.0
6	84.0
7	157.0
8	212.0
9	207.0
10	174.0
11	144.0
12	163.0
13	214.0
14	242.0
15	376.0
16	515.0
17	650.0
18	784.0
19	980.0
20	1250.0
21	1638.0
22	2094.0
23	2483.0
24	3013.0
25	3415.0
26	3842.0
27	4365.0
28	5084.0
29	6012.0
30	7173.0
31	8369.0
32	9605.0
33	11044.0
34	12854.0
35	15260.0
36	19362.0
37	24346.0
38	22435.0
39	11054.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.5349198166287	0.3858114512829894	0.05931296736678141	0.01995576472153393
8	99.37693667924988	0.4661888369669455	0.0720624837166503	0.08481200006651922
9	97.54821257324043	1.3597636350534095	0.6613118697997218	0.4307119219064407
10	47.90824782842477	30.828330533983006	14.275578024268427	6.9878436133237996
11	34.881568079645675	34.408727321104884	15.951862260877277	14.757842338372166
12	34.66427197490008	14.577686129080538	19.557758080698896	31.200283815320486
13	24.90407647267566	15.194730305180979	23.22347409510291	36.677719127040454
14	22.785553923334902	18.12134482663045	32.924970204273954	26.16813104576069
15	32.09738701506466	17.25992089943563	26.126516464471404	24.516175621028307
16	36.01206984535511	18.643362694415476	23.720352332985733	21.62421512724368
17	28.640956989783756	20.36596655192102	29.322778951102833	21.67029750719239
18	26.66910574513847	20.743808066697706	30.45854675269962	22.1285394354642
19	33.09342537512208	23.54168516381071	21.752086477847822	21.61280298321939
20	27.96651076083157	29.539439737678723	21.12163429262582	21.372415208863885
21	25.82885714444093	22.696910736755747	29.829987971108483	21.64424414769483
22	25.185283732171467	21.06885293155727	25.4419370395623	28.303926296708966
23	26.463007001147453	21.081602447907137	24.5744156009734	27.880974949972003
24	26.718699763855476	20.456435215468023	30.76641647911839	22.05844854155811
25	32.73779497441753	19.79744674246246	24.51426575829975	22.950492524820255
26	23.067029598132862	19.153689649246324	28.35522194441826	29.424058808202545
27	21.931460025746883	17.932370044835086	29.329249345230167	30.806920584187864
28	21.824802122534166	26.16645019482466	29.413527824957537	22.59521985768364
29	30.81704855488765	19.17407892110108	30.026284852383384	19.982587671627886
30	23.13249538425713	17.142841301611767	40.0036593276817	19.721003986449396
31	23.011106859858376	23.478005312217544	34.14458325708804	19.36630457083604
32	31.97161137098877	16.124252967322004	33.54308512704411	18.361050534645116
33	25.026930380801137	16.96891623263407	34.60026430641776	23.40388908014703
34	20.74094614830015	23.778492596084522	39.865232100271754	15.615329155343574
35	19.493189280326558	19.713927588960868	45.84368621883042	14.949196911882154
36	19.909941384263032	27.87103428771066	36.90351417147737	15.315510156548937
37	21.201332963065813	19.567897806164954	42.98861427381283	16.2421549569564
38	27.8530765638551	20.19669105383709	35.62843292045631	16.321799461851498
39	22.018970189701896	18.543138300235462	35.80623306233062	23.63165844773202
40	16.795333389154038	13.875352368193363	37.6192469787044	31.7100672639482
41	13.959158111342235	13.712563465968822	35.69695139681048	36.631327025878456
42	23.972222530614058	18.057020416773064	37.6616744196374	20.309082632975475
43	15.965947609997889	14.598961980017561	43.021149379299615	26.41394103068494
44	16.375963054365567	15.304138557270747	36.556234575810414	31.763663812553272
45	19.875555555555554	17.552777777777777	43.656666666666666	18.915000000000003
46	18.2179713622377	19.049444018617876	37.32295797647215	25.40962664267227
47	17.155087804905097	24.204432664422797	38.24101850362919	20.39946102704291
48	18.72685172331458	24.636707405782694	38.45487508953906	18.18156578136366
49	16.74347072623021	19.732420971687443	33.45183633212787	30.07227196995447
50	24.62243782226687	18.519279121269474	37.60968868118267	19.248594375280987
51	18.818564462410134	24.85807538546225	38.325484156153216	17.9978759959744
52	18.3886098082207	18.298687241542005	43.73234159473787	19.58036135549943
53	18.252090760670374	18.215439976010394	44.65675984851009	18.875709414809137
54	16.48328443140393	23.252311727571797	37.18547168133725	23.078932159687028
55	18.53368750972503	24.75604063396092	37.00902482939516	19.70124702691889
56	24.883072445091265	19.145567862419874	35.395998355793054	20.57536133669581
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	19.5
9	38.0
10	132.5
11	227.0
12	370.5
13	668.5
14	823.0
15	986.5
16	1150.0
17	1342.5
18	1535.0
19	1790.5
20	2046.0
21	2274.5
22	2994.5
23	3486.0
24	4215.5
25	4945.0
26	5436.5
27	5928.0
28	6167.5
29	6429.5
30	6452.0
31	5838.0
32	5224.0
33	4644.0
34	4064.0
35	3658.0
36	3252.0
37	3069.0
38	2926.5
39	2967.0
40	3102.5
41	3238.0
42	3568.0
43	3898.0
44	6626.5
45	9355.0
46	9172.5
47	7388.0
48	5786.0
49	5755.0
50	5724.0
51	6145.0
52	6566.0
53	6813.5
54	7391.5
55	7722.0
56	8312.5
57	8903.0
58	10231.5
59	11560.0
60	11551.5
61	11543.0
62	10381.5
63	8283.0
64	7346.0
65	6611.0
66	5876.0
67	5116.5
68	4357.0
69	3808.5
70	3260.0
71	2818.5
72	1889.5
73	1402.0
74	1127.5
75	853.0
76	666.5
77	480.0
78	361.5
79	180.5
80	118.0
81	81.5
82	45.0
83	31.0
84	17.0
85	13.0
86	9.0
87	4.5
88	0.5
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.02605335949755819
14	0.0022173071912815483
15	0.20787254918264517
16	0.06374758174934451
17	0.0
18	0.0016629803934611609
19	0.10587641838369392
20	0.0898009412469027
21	0.0
22	0.0
23	0.0
24	5.543267978203871E-4
25	0.0011086535956407741
26	0.008869228765126193
27	0.10144180400113083
28	0.1313754510834317
29	0.03713989545396593
30	0.021064418317174708
31	0.03325960786922322
32	0.10310478439459199
33	0.16906967333521805
34	0.04933508500601445
35	0.05266104579293677
36	0.03991152944306786
37	0.19346005243931508
38	0.2899129152600624
39	0.18126486288726656
40	0.6951258044667653
41	0.8664127849932649
42	0.14024467984855793
43	0.24445811783879068
44	0.4944595036557853
45	0.22117639233033443
46	0.19789466682187817
47	0.031042300677941677
48	0.17184130732431996
49	0.519958536355523
50	0.12749516349868903
51	0.3048797388012129
52	0.13525573866817445
53	0.17793890210034424
54	0.247784078625713
55	0.25000138581699455
56	0.2067638955870044
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	180399.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.08947664696524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.05822040396149	53.23157876918182
2	9.587898049099653	12.289667362225117
3	3.1949675114037075	6.142913871297617
4	1.4078215105578842	3.6090617529597933
5	0.7616313452754193	2.440627715831285
6	0.5003673858842981	1.9240970335520862
7	0.3209440152847199	1.4398393780801315
8	0.2212708764619535	1.134490773572952
9	0.1804053758527357	1.04058775104308
>10	0.6977002193507323	7.60031352635209
>50	0.04485215665264565	1.9093408167460812
>100	0.018937577253339274	2.1878528930596617
>500	0.001993429184562029	0.9243279229948409
>1k	0.0029901437768430432	4.125300433103444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	4195	2.3254009168565237	No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG	1132	0.6274979351326782	No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	1131	0.6269436083348577	No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	913	0.5061003664100134	No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	534	0.2960105100360867	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	351	0.19456870603495585	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	296	0.16408073215483457	No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	294	0.1629720785591938	No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG	282	0.15632015698534915	No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	244	0.13525573866817445	No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG	226	0.12527785630740748	No Hit
TAGCATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	212	0.11751728113792205	No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	187	0.10365911119241238	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.543267978203871E-4	0.0	0.0	0.0	0.0
8	5.543267978203871E-4	0.0	0.0	0.0	0.0
9	5.543267978203871E-4	0.0	0.0	0.0	0.0
10	0.0060975947760242575	0.0	0.0	0.0	0.0
11	0.011640862754228128	0.0	0.0	0.0	0.0
12	0.014412496743330063	0.0	0.0	0.0	0.0
13	0.017184130732432	0.0	0.0	0.0	0.0
14	0.017738457530252386	0.0	0.0	0.0	0.0
15	0.02272739871063587	0.0	0.0	0.0	0.0
16	0.026607686295378578	0.0	0.0	0.0	0.0
17	0.031596627475762064	0.0	0.0	0.0	0.0
18	0.036585568656145546	0.0	0.0	0.0	0.0
19	0.04379181702781058	0.0	0.0	0.0	0.0
20	0.04656345101691251	0.0	0.0	0.0	0.0
21	0.05266104579293677	0.0	0.0	0.0	0.0
22	0.05654133337767948	0.0	0.0	0.0	0.0
23	0.06153027455806296	0.0	0.0	0.0	0.0
24	0.06541056214280568	0.0	0.0	0.0	0.0
25	0.07206248371665032	0.0	0.0	0.0	0.0
26	0.07926873208831535	0.0	0.0	0.0	0.0
27	0.08370334647087845	0.0	0.0	0.0	0.0
28	0.08813796085344154	0.0	0.0	0.0	0.0
29	0.09146392164036386	0.0	0.0	0.0	0.0
30	0.09478988242728618	0.0	0.0	0.0	0.0
31	0.09589853602292696	0.0	0.0	0.0	0.0
32	0.0981158432142085	0.0	0.0	0.0	0.0
33	0.10144180400113083	0.0	0.0	0.0	0.0
34	0.10199613079895122	0.0	0.0	0.0	0.0
35	0.10310478439459199	0.0	0.0	0.0	0.0
36	0.10476776478805315	0.0	0.0	0.0	0.0
37	0.10809372557497547	0.0	0.0	0.0	0.0
38	0.10920237917061625	0.0	0.0	0.0	0.0
39	0.11308266675535895	0.0	0.0	0.0	0.0
40	0.11696295434010166	0.0	0.0	0.0	0.0
41	0.11807160793574244	0.0	0.0	0.0	0.0
42	0.1236148759139463	0.0	0.0	0.0	0.0
43	0.12583218310522787	0.0	0.0	0.0	0.0
44	0.12694083670086864	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTCG	15	0.008154757	50.405617	41
CGTCACG	20	4.0167323E-4	50.391464	37
ATGCTCG	165	0.0	50.3067	36
TATGCTC	170	0.0	50.278507	35
TATGCCT	85	0.0	50.278507	35
TTGTCGA	15	0.008263988	50.236282	34
CGATTGT	15	0.008263988	50.236282	44
ATATGCC	135	0.0	50.236282	34
ATATGCA	15	0.008263988	50.236282	34
GTTAAGC	20	4.078359E-4	50.236282	34
CGATTCA	95	0.0	50.194126	49
CGATTAA	185	0.0	50.194126	49
ACTGCTA	15	0.008300641	50.180088	45
TCGTGCG	15	0.008300641	50.180088	46
CTACCAA	25	2.0365702E-5	50.180088	46
CGTTCGT	25	2.0433212E-5	50.152042	42
GTCAGCT	15	0.008319011	50.15204	42
ATGATTT	30	1.0331478E-6	50.06808	33
CGCAGTA	15	0.00846707	49.92877	13
CTTTCTA	15	0.00846707	49.92877	13
>>END_MODULE