FastQCFastQC Report
Fri 17 Jun 2016
SRR1526231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27216
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1010.37110523221634334No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC700.257201646090535No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC660.24250440917107582No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG470.1726925338036449No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC440.16166960611405057No Hit
CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.1432980599647266No Hit
CCGCTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC370.13594944150499708No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG340.1249265138154027No Hit
CCGCTAGGGCAGAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.11390358612580835No Hit
CCGCTAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.10655496766607878No Hit
CCGCTAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTC290.10655496766607878No Hit
CCGCTAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG280.102880658436214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATTG150.0080179450.5037339
GCCCCCG251.9158377E-550.5037339
CCCCGAC203.948511E-450.40968741
TGCTGCA150.00813718250.31598344
GATGCTT204.0218804E-450.22263349
TGCCTAC150.008197295550.22263343
TACCCAG150.008197295550.22263343
CCAAGCG150.008197295550.22263349
TTGCTGC150.008197295550.22263343
AGATGCT204.0589692E-450.1296348
ACCAAGC150.00825773950.12962748
ATTGTAA150.00825773950.12962742
CAGATGC150.00825773950.12962747
CTACCCA150.00825773950.12962742
CAATGCT150.00831851350.0369713
TTGCAGA150.00831851350.0369714
ATCAGAC150.00831851350.0369734
ATGCTTA204.0963292E-450.0369750
ATGCTCC204.0963292E-450.0369715
TGCAGAC150.00831851350.0369715
Produced by FastQC (version 0.11.5)