FastQCFastQC Report
Fri 17 Jun 2016
SRR1526230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123601
Sequences flagged as poor quality0
Sequence length56
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG15301.237854062669396No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG4220.34142118591273535No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3670.29692316405207075No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG3320.26860624104982966No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3050.246761757590958No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2430.19660035112984525No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2350.19012791158647585No Hit
TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG2200.17799208744265824No Hit
TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1740.14077556006828423No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1570.12702162603862427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGAG150.00813767350.42283639
CCGTCCA150.00813767350.42283640
CGTCTCA950.050.38159641
TGCTCGT650.050.34041637
TGCCCGT309.925989E-750.34041237
ATGCTCG650.050.2993136
GTAAGAC150.0082835550.1968234
CGATTAA750.050.13553249
ACGATTA750.050.11513548
CAAGATT204.122167E-450.11513547
ACGATTC301.0238182E-650.1151348
ATGAGAG150.00833707550.1151348
AAGATTC150.00833707550.1151348
GATTCAG451.3278623E-1050.03371450
AAAGTCT150.00839085750.0337150
CGGTTGA204.1972304E-449.9323114
TGGTTGA4850.049.9323114
GGTTGAT5000.049.9323115
CAGCCGT150.00845844849.93230427
TTACTAT150.00845844849.93230427
Produced by FastQC (version 0.11.5)