FastQCFastQC Report
Fri 17 Jun 2016
SRR1526229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87955
Sequences flagged as poor quality0
Sequence length56
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4480.5093513728611222No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1720.19555454493775226No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1420.16144619407651642No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1400.15917230401910068No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1320.15007674378943778No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1280.14552896367460633No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1110.12620089818657268No Hit
ACAATAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT1070.12165311807174123No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG930.10573588766983116No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.10573588766983116No Hit
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA920.1045989426411233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCTC150.00805849550.5352238
ACATAGA150.00807691750.5060640
GCCGCAT203.960585E-450.50605839
AAGACTA150.00824412950.2451236
ATGCTCG150.00824412950.2451236
TTAGAGC150.00826286650.21629335
ATAGAAT150.00826286650.21629342
CGTTGTG150.00830043550.1587434
GTTAGAG150.00830043550.1587434
ACGATTC150.00831926650.13001348
ACCAAGC150.00831926650.13001348
TTACCGA150.00831926650.13001349
ACGATTA252.0399368E-550.1300148
AGCAAGG150.0083381350.1013245
TACCGAA150.00839491250.0154350
ATGCTAG150.00841390349.98686615
ATTCAAG150.00843292649.95833633
TCCCAAT150.00843292649.95833633
TCGTTGT204.1797527E-449.95833233
TTCGTTG204.1797527E-449.95833232