##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526229_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 87955 Sequences flagged as poor quality 0 Sequence length 56 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.829708373600134 39.0 38.0 39.0 35.0 40.0 2 37.13404581888466 39.0 38.0 40.0 33.0 40.0 3 37.396157125802965 39.0 38.0 39.0 34.0 40.0 4 37.48167813086237 39.0 38.0 39.0 34.0 40.0 5 36.44615996816554 39.0 36.0 39.0 30.0 40.0 6 36.4235802398954 39.0 37.0 39.0 31.0 40.0 7 36.17065544880905 39.0 36.0 39.0 30.0 40.0 8 36.30069922119266 39.0 36.0 39.0 30.0 40.0 9 36.262952646239555 39.0 36.0 39.0 30.0 40.0 10 35.839326928543 39.0 35.0 39.0 29.0 40.0 11 35.07004718321869 38.0 34.0 39.0 27.0 40.0 12 32.93892331305781 38.0 31.0 39.0 17.0 40.0 13 32.70309817520323 37.0 30.0 39.0 17.0 40.0 14 32.910897618100165 38.0 31.0 39.0 18.0 40.0 15 32.84978682280712 38.0 30.0 39.0 17.0 40.0 16 32.92442726394179 38.0 31.0 39.0 18.0 40.0 17 33.019544085043485 37.0 31.0 39.0 20.0 40.0 18 32.477312261952136 36.0 30.0 39.0 17.0 40.0 19 33.378420783355125 37.0 31.0 39.0 22.0 40.0 20 33.25741572394975 37.0 31.0 39.0 22.0 40.0 21 34.180330851003355 38.0 33.0 39.0 23.0 40.0 22 33.96273094195896 38.0 33.0 39.0 23.0 40.0 23 34.26250923767836 38.0 33.0 39.0 24.0 40.0 24 33.97425956455005 38.0 33.0 39.0 23.0 40.0 25 34.0927406059917 38.0 33.0 39.0 23.0 40.0 26 34.85912114149281 38.0 35.0 39.0 27.0 40.0 27 34.7639247342391 38.0 35.0 39.0 26.0 40.0 28 34.69211528622591 38.0 34.0 39.0 26.0 40.0 29 34.58852822466034 38.0 34.0 39.0 26.0 40.0 30 34.4223523392644 38.0 34.0 39.0 25.0 40.0 31 34.826445341367744 38.0 35.0 39.0 27.0 40.0 32 34.483099312148255 38.0 34.0 39.0 26.0 40.0 33 34.63895173668353 38.0 35.0 39.0 27.0 40.0 34 34.51176169632198 38.0 34.0 39.0 27.0 40.0 35 34.36821101699733 38.0 34.0 39.0 26.0 40.0 36 33.50775964982093 36.0 33.0 39.0 25.0 40.0 37 33.295867204820645 36.0 32.0 39.0 24.0 40.0 38 33.023091353533054 36.0 32.0 39.0 23.0 40.0 39 32.78595872889546 36.0 32.0 39.0 21.0 40.0 40 29.380296742652494 35.0 23.0 38.0 6.0 39.0 41 25.932317662441022 31.0 13.0 38.0 4.0 39.0 42 30.289102381899834 34.0 29.0 38.0 4.0 39.0 43 28.702177249729974 33.0 25.0 38.0 4.0 39.0 44 28.731044284008867 33.0 25.0 38.0 4.0 39.0 45 29.879847649366152 34.0 28.0 38.0 4.0 39.0 46 29.58968790858962 35.0 27.0 38.0 4.0 40.0 47 29.104655789892558 35.0 26.0 38.0 4.0 40.0 48 29.20859530441703 35.0 27.0 38.0 4.0 40.0 49 26.590574725712013 33.0 13.0 38.0 4.0 39.0 50 28.865851856062758 34.0 26.0 38.0 4.0 39.0 51 29.08861349553749 35.0 27.0 39.0 4.0 40.0 52 28.804093002103347 35.0 26.0 39.0 4.0 40.0 53 28.45436871127281 35.0 24.0 39.0 4.0 40.0 54 27.47788073446649 34.0 16.0 39.0 4.0 40.0 55 27.833289750440567 34.0 21.0 39.0 4.0 40.0 56 27.04735376044568 34.0 16.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.9564075101478693 1 2 1.969034676570125 1 3 1.6230669455746636 1 4 1.5324482504021475 1 5 2.8072452688311316 1 6 2.926739255823229 1 7 3.1534456433129563 1 8 3.071131121069726 1 9 3.114443444517569 1 10 3.3163102448921435 1 11 -0.7388001693936985 1 12 -11.169511109192559 1 13 -7.405738958905108 1 14 -4.272228105530534 1 15 -3.928958777350932 1 16 -21.221904017820584 1 17 -3.51666831012259 1 18 -10.418438059660193 1 19 -4.426150577320705 1 20 -4.260317769474678 1 21 -4.753461337005966 1 22 -1.2266367314884477 1 23 -2.181961064556674 1 24 -2.6378730716210796 1 25 -1.8445522841098452 1 26 3.834571920294394 1 27 3.5135366646457484 1 28 3.7495826457733727 1 29 3.538723745245953 1 30 3.717745506777348 1 31 3.5294423173764002 1 32 3.262040426116556 1 33 3.494895267252865 1 34 2.859726774695453 1 35 3.3086689432126875 1 36 4.121884235638056 1 37 4.488346985975667 1 38 3.759971333203307 1 39 4.477810970619515 1 40 7.333355523108935 1 41 -8.175882740544441 1 42 -8.810179327278888 1 43 -15.53274866992933 1 44 -21.23382647831339 1 45 -11.263904702542824 1 46 -16.75503067052145 1 47 -22.464262819839682 1 48 -14.828357040839359 1 49 -18.884604430363993 1 50 -14.353602270203616 1 51 -11.434366660172074 1 52 -11.115719384801206 1 53 -11.048272842353358 1 54 -10.329926944106532 1 55 -10.522700408163569 1 56 -6.385508794460819 2 1 1.12892785986147 2 2 1.989233651547515 2 3 1.7552647146431042 2 4 1.7991802371371506 2 5 2.5435280544808165 2 6 3.3362207149730594 2 7 3.334558094322901 2 8 3.1180862009611943 2 9 3.2407624074358665 2 10 3.0747516075692616 2 11 2.7093457455897223 2 12 0.3030544621068074 2 13 0.9914573027771354 2 14 4.934736018678091 2 15 4.593652015534207 2 16 3.42627204488668 2 17 3.78503503627476 2 18 -1.231371433190489 2 19 3.279607637934042 2 20 3.299902308909399 2 21 3.3164060130091073 2 22 3.6121483152405247 2 23 3.4056180732184274 2 24 2.067960512943287 2 25 3.2789780143526173 2 26 2.803142918184051 2 27 2.867470942607767 2 28 2.810857894492095 2 29 2.566384281875436 2 30 3.3863466523908556 2 31 1.723593658950115 2 32 2.736867076704442 2 33 2.781750859655638 2 34 2.179066539543207 2 35 1.9920424735111553 2 36 3.7207687698557166 2 37 3.3150729431659016 2 38 3.6711862864743345 2 39 3.4990651159315433 2 40 6.900961793835499 2 41 -3.40651282797284 2 42 -5.055581799392243 2 43 -9.921729676863304 2 44 -12.471315172916949 2 45 -4.534555893377917 2 46 -8.958377067868447 2 47 -16.99388295851921 2 48 -7.133150528931008 2 49 -10.918068403833995 2 50 -6.044361992844557 2 51 -1.900224362915516 2 52 -1.8660208620396759 2 53 -1.1764006687024704 2 54 -0.452929657401679 2 55 -1.0307649242926011 2 56 1.894412821457177 3 1 1.6139509013361248 3 2 2.803109739617149 3 3 2.082197638350216 3 4 2.1474905289958315 3 5 3.4340810498725176 3 6 3.5181203565501207 3 7 3.8531192822389286 3 8 3.717548566552594 3 9 3.846753190846009 3 10 3.4269021452036696 3 11 4.621532125518037 3 12 6.695271564001331 3 13 5.483520794840629 3 14 5.610875291591558 3 15 5.254174288959707 3 16 6.139021660862106 3 17 6.38193723801567 3 18 6.692079667679966 3 19 5.703058738804501 3 20 5.678934566973918 3 21 4.875673806147866 3 22 5.19548164857386 3 23 4.652929901175412 3 24 5.0007562118680156 3 25 4.912234542770442 3 26 3.8350169581226083 3 27 3.884624040406024 3 28 3.4873811919831326 3 29 3.4879152598580276 3 30 4.1870378966304855 3 31 2.993690945176432 3 32 3.687199898005005 3 33 3.6568148637519045 3 34 2.6768904002702882 3 35 3.187766999880843 3 36 4.012627448811173 3 37 4.244540429084999 3 38 4.512456127744176 3 39 4.491384624380082 3 40 8.362431327885673 3 41 8.324413951341036 3 42 4.875549793029673 3 43 -0.45872404450283355 3 44 1.9965547707594453 3 45 4.670897255623618 3 46 1.9489450007439437 3 47 -2.729802057341537 3 48 2.3312631935472297 3 49 4.337691964829599 3 50 2.2806508079746948 3 51 3.5947833175760344 3 52 3.5212039203463945 3 53 3.5012747025212896 3 54 4.026845048179478 3 55 3.5090456235824625 3 56 4.354218249004539 4 1 1.3044270918123146 4 2 2.5332684697758836 4 3 1.8123563685089508 4 4 1.877649259154559 4 5 3.251541367332834 4 6 3.4149457533755196 4 7 3.5197859489056 4 8 3.558818407822436 4 9 3.2435785876714007 4 10 3.1570608753624043 4 11 4.280262284248195 4 12 6.64765251638228 4 13 6.3962192075390405 4 14 6.206113386829653 4 15 6.055761590547004 4 16 6.123148644989087 4 17 5.921619777698208 4 18 5.946047921648219 4 19 5.115757151502912 4 20 5.147188535227887 4 21 3.7169436474177076 4 22 4.362148315240525 4 23 4.073564821810336 4 24 4.476946688058497 4 25 4.729694860230758 4 26 3.525493148598798 4 27 3.9084335642155494 4 28 3.2334129380148795 4 29 3.0752168471596093 4 30 3.2346569442495365 4 31 2.485754437239926 4 32 3.234818945624049 4 33 2.037767244704284 4 34 1.9784777018575923 4 35 2.2195130316268745 4 36 3.4729449091286355 4 37 3.8953340798786513 4 38 4.321979937267987 4 39 3.8326544656499237 4 40 7.386240851695199 4 41 10.4910806180077 4 42 6.407295824775705 4 43 8.533339447560657 4 44 6.766396040600714 4 45 6.043913128639492 4 46 5.8378338896328295 4 47 6.44480111726164 4 48 6.44237430465834 4 49 8.932930060067697 4 50 6.0663650936889795 4 51 5.301132523925244 4 52 5.545013444155924 4 53 5.826671527918116 4 54 5.598273619608051 4 55 4.326505941042779 4 56 3.9494563442426376 5 1 1.4148876694860064 5 2 2.5830993310821526 5 3 1.8520389081914885 5 4 1.8328925586601557 5 5 3.0909897149779084 5 6 3.599176822852492 5 7 3.717027687130944 5 8 3.679297200432373 5 9 3.2870342212909733 5 10 3.334005970340286 5 11 4.406595877794906 5 12 6.373387619166564 5 13 6.188178614252543 5 14 5.916756113865819 5 15 6.370489667570165 5 16 5.657366703797315 5 17 5.414463909886603 5 18 5.831293823287563 5 19 4.547190927198983 5 20 5.105164075170638 5 21 3.8863425545215335 5 22 4.288377823437244 5 23 3.7895419230333403 5 24 4.457040364873485 5 25 4.067581927626023 5 26 3.417764811362261 5 27 3.497296431766941 5 28 3.6695305544603656 5 29 3.4589014685491506 5 30 3.7069442198154974 5 31 2.4692308879294913 5 32 3.6324249825743493 5 33 2.500947052146387 5 34 2.5255763227267067 5 35 2.9702286184080364 5 36 4.091472101426319 5 37 4.322344228200272 5 38 4.941678089753022 5 39 5.031587590812556 5 40 8.596493258668922 5 41 11.330398858965289 5 42 6.679479014991681 5 43 8.764018604469321 5 44 8.168425704925465 5 45 6.58112364125984 5 46 6.350454238318758 5 47 6.462885946821878 5 48 6.018356610148846 5 49 9.465326625251144 5 50 6.611772327620805 5 51 6.080341475265339 5 52 6.275042067627165 5 53 7.2548526364270955 5 54 7.010581712244015 5 55 6.821561886918399 5 56 6.476908855301705 6 1 1.1103794727646914 6 2 2.3717605332679454 6 3 1.5353722415248185 6 4 1.7927286242339235 6 5 2.831700097491563 6 6 3.137564800994568 6 7 3.228912933032582 6 8 2.784215233219257 6 9 3.0765150956079097 6 10 2.718568811870341 6 11 3.4604210144069256 6 12 5.954398548128317 6 13 5.941457302777138 6 14 5.981510212226478 6 15 6.206555241340656 6 16 5.404497851338299 6 17 5.9982070792855104 6 18 6.062714588314886 6 19 4.582026992772754 6 20 4.678934566973918 6 21 4.200276980751042 6 22 4.012148315240523 6 23 3.961263234508749 6 24 4.5007562118680156 6 25 4.065805971341867 6 26 3.1612074343130843 6 27 2.448116103898087 6 28 2.8151589697609083 6 29 3.3645025614453274 6 30 3.47989503948763 6 31 2.6063893578748463 6 32 2.141168151973254 6 33 2.3524497843868275 6 34 2.9169697653496556 6 35 2.8310209681348084 6 36 3.9495322107159367 6 37 4.096524556069127 6 38 3.762852953141003 6 39 3.7151941481896102 6 40 7.840209105663455 6 41 4.991874268801354 6 42 5.789041856521731 6 43 7.580958495179708 6 44 6.521157945362621 6 45 6.217325827052189 6 46 6.686246588045531 6 47 6.734880482341005 6 48 6.308247320531354 6 49 7.778168155305792 6 50 5.0731111254350125 6 51 4.626926174718893 6 52 4.079140428282905 6 53 4.308814385060973 6 54 5.197876794211226 6 55 3.593966258503098 6 56 4.553821423607715 7 1 1.1212769086621321 7 2 2.293341729849139 7 3 1.7110132671658462 7 4 1.6957200772253813 7 5 2.9133240291155005 7 6 3.3429066813364443 7 7 3.217588146707797 7 8 3.157719506723531 7 9 3.4480962921891063 7 10 3.2687824870840174 7 11 3.9206774246633387 7 12 6.367432736162499 7 13 6.269235080554914 7 14 5.993048673764935 7 15 6.638179172964588 7 16 6.1927457145861595 7 17 5.971070327148755 7 18 5.963141938742236 7 19 4.615146650892413 7 20 5.324233712273067 7 21 4.1870291175031795 7 22 4.806592759684968 7 23 4.4136136618591735 7 24 4.4110126221244315 7 25 4.225421355957252 7 26 3.2000963232019686 7 27 2.960936616718598 7 28 3.0130222176241546 7 29 3.1527504246931883 7 30 3.8726300822226776 7 31 2.735449186934673 7 32 3.33433054513565 7 33 2.4364241433611866 7 34 2.5248757482556385 7 35 3.463713275827118 7 36 3.1634210996048253 7 37 3.0681057526503253 7 38 3.461784577072624 7 39 3.6775873105827728 7 40 5.541918507372856 7 41 -1.6871855602584773 7 42 3.507417924897805 7 43 5.388650802872014 7 44 4.820730594935274 7 45 3.6995907843171487 7 46 5.002058553857498 7 47 5.7415044139649325 7 48 5.466794329078365 7 49 7.592270719408354 7 50 5.2605042878281765 7 51 5.729703952496667 7 52 5.375294274436751 7 53 6.0513357525823395 7 54 6.0976631189975485 7 55 6.024308138844976 7 56 6.968992363778657 8 1 1.357648380327717 8 2 2.402082662119483 8 3 1.9150641182755237 8 4 1.999031145242327 8 5 3.2029886128977267 8 6 3.459763680546388 8 7 3.2896272187468654 8 8 3.085895905488165 8 9 2.934988484963654 8 10 3.0921682516462496 8 11 4.051037260905524 8 12 6.252227679780972 8 13 5.802381672525037 8 14 5.867644666007987 8 15 5.597311543861661 8 16 5.892523061422331 8 17 5.49071408208663 8 18 6.046888257782673 8 19 5.503245480167713 8 20 5.010867340083163 8 21 3.6721257202468394 8 22 3.7739130211228797 8 23 3.9610531504751307 8 24 4.317282822512276 8 25 4.350609892910498 8 26 3.6221318040609845 8 27 3.1035782887720345 8 28 3.4990525271838777 8 29 4.026197239316474 8 30 3.6030042831851077 8 31 2.7187142878468364 8 32 3.364137339648323 8 33 2.595657067299989 8 34 2.9285243871983937 8 35 2.898317886902319 8 36 2.952403359175321 8 37 3.5690035476657656 8 38 3.356060236054166 8 39 3.1706563330635547 8 40 6.112197901181659 8 41 -0.7740921177532698 8 42 4.105708523188401 8 43 5.772835245879989 8 44 5.470877833317804 8 45 3.904790813046592 8 46 5.551652750510517 8 47 5.790272639203749 8 48 5.124913987198024 8 49 7.94693566230859 8 50 5.028083114230533 8 51 6.310469592085841 8 52 6.201409335845927 8 53 6.60538301251195 8 54 7.218254945471731 8 55 6.090744969987693 8 56 6.8831631603023915 9 1 1.2871493842691208 9 2 2.3032502087841706 9 3 1.812215899341929 9 4 2.0303392437029544 9 5 3.0377472951316875 9 6 3.309172470611088 9 7 3.4463908091387765 9 8 3.547932047378552 9 9 3.3960578684692706 9 10 3.6053682218998375 9 11 4.070892990808105 9 12 6.45078497880678 9 13 6.1286254443700585 9 14 6.469563309571608 9 15 6.511864975853928 9 16 6.223303161072806 9 17 6.044741002589344 9 18 5.7071098685508765 9 19 5.311009293657712 9 20 5.143536336885422 9 21 3.997179635618302 9 22 4.0170155718776925 9 23 4.446440225659188 9 24 4.6703727310420575 9 25 4.050245499365467 9 26 3.4616499121891877 9 27 3.828647077349416 9 28 3.2105866983744775 9 29 3.5254907620352967 9 30 3.865293269576128 9 31 2.894589947845347 9 32 3.4042949956310764 9 33 3.0656503743573253 9 34 2.294182154730187 9 35 3.0527761303766994 9 36 3.5724673139607717 9 37 4.499843140139923 9 38 3.7995048705451318 9 39 4.0019198119064185 9 40 7.535489341651655 9 41 5.43390966703144 9 42 5.9198678152238 9 43 7.154704807864071 9 44 6.991216942412766 9 45 4.3824438211524885 9 46 5.517072546747595 9 47 5.9839217802761056 9 48 5.43464850047236 9 49 7.756781724627324 9 50 5.80312587469755 9 51 5.993224109822133 9 52 5.422945738017415 9 53 6.3711300192792635 9 54 6.197655555273169 9 55 6.141016405995725 9 56 4.698437942781755 10 1 1.1590636832910093 10 2 2.4827254455486454 10 3 1.738003820472187 10 4 2.032202308444454 10 5 3.244857992228411 10 6 3.2608104150296526 10 7 3.267070827769423 10 8 3.217548566552594 10 9 3.2690589552570373 10 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34 1.5419697653496556 115 35 2.2579817524485364 115 36 2.431394955813971 115 37 2.0715245560691287 115 38 2.4417745217684548 115 39 3.091664736424903 115 40 5.908836556643848 115 41 5.683050739389589 115 42 1.1998261702472242 115 43 -5.440610132271274 115 44 -6.3190381330687515 115 45 1.667816023130623 115 46 -2.8853220394054517 115 47 -2.362668537266842 115 48 -2.2633213069196216 115 49 -13.340459295674602 115 50 -2.7567908353492996 115 51 -2.2794463743007185 115 52 -1.976741924658274 115 53 -2.6534405168998134 115 54 -7.5330055587299505 115 55 -2.9579945258106264 115 56 -0.6378452430589512 116 1 1.0353794727646957 116 2 1.9334271999346129 116 3 1.2920389081914863 116 4 1.5527286242339287 116 5 2.648366764158233 116 6 2.975898134327899 116 7 2.623912933032578 116 8 2.7442152332192578 116 9 2.558181762274579 116 10 2.8602354785370068 116 11 0.1070876810735939 116 12 -11.437268118538352 116 13 -5.358542697222866 116 14 -1.7184897877735246 116 15 -4.173444758659343 116 16 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-7.447123205788774 116 55 -2.862700408163569 116 56 -1.4678452430589495 117 1 0.20537947276469737 117 2 1.3500938666012772 117 3 0.6453722415248251 117 4 0.8427286242339278 117 5 2.035033430824896 117 6 1.929231467661232 117 7 2.36391293303258 117 8 2.364215233219255 117 9 2.0248484289412474 117 10 1.8869021452036705 117 11 -1.55624565225974 117 12 -8.860601451871688 117 13 -7.9352093638895305 117 14 -9.881823121106859 117 15 -8.320111425326012 117 16 -12.20383548199504 117 17 -12.31679292071449 117 18 -13.400618745018448 117 19 -5.669639673893911 117 20 -5.22106543302608 117 21 0.07694364741770698 117 22 0.7621483152405233 117 23 0.2662632345087488 117 24 0.14742287853468383 117 25 0.29413930467519833 117 26 2.804540767646415 117 27 3.0081161038980895 117 28 2.6718256364275774 117 29 3.0761692281119934 117 30 2.949895039487629 117 31 2.5330560245415157 117 32 2.527834818639924 117 33 2.29078311772016 117 34 2.8886364320163196 117 35 2.7560209681348056 117 36 3.3811988773826016 117 37 2.9915245560691233 117 38 3.6911862864743377 117 39 4.275194148189605 117 40 7.740209105663453 117 41 3.355207602134687 117 42 -1.3342914768116003 117 43 -9.092374838153628 117 44 -10.535508721304044 117 45 -0.5376741729478063 117 46 -4.337086745287802 117 47 -4.310119517658997 117 48 -4.021752679468644 117 49 -9.985165178027543 117 50 -4.528555541231654 117 51 -3.701407158614444 117 52 -3.590859571717097 117 53 -3.8095189482723626 117 54 -10.267123205788774 117 55 -5.64270040816357 117 56 -3.7845119097256195 118 1 0.2038979912832133 118 2 1.343674113514858 118 3 0.8335203896729695 118 4 0.9217409699129391 118 5 2.105156887615024 118 6 2.548984554080988 118 7 2.49774009352641 118 8 2.328659677663701 118 9 2.597194107953591 118 10 2.4454206637221887 118 11 0.621532125518037 118 12 -6.944058241995144 118 13 -6.767801956482124 118 14 -8.382070034687104 118 15 -7.293444758659344 118 16 -10.892724370883926 118 17 -10.523706500961403 118 18 -10.276174300574002 118 19 -4.055318686239591 118 20 -5.030941976235958 118 21 1.6675609313683282 118 22 2.2757285621541072 118 23 2.0479916295704754 118 24 1.2847068291519719 118 25 1.9272257244282898 118 26 2.767997557769874 118 27 2.6333012890832705 118 28 2.3947885993905373 118 29 2.4499963886058183 118 30 2.706438249364176 118 31 1.511327629479787 118 32 -3.8613009838292136 118 33 1.768807809078183 118 34 1.727154950534839 118 35 0.8967617088755517 118 36 2.323914926765319 118 37 2.8221418400197464 118 38 3.29464307659779 118 39 3.3547003210291138 118 40 7.078480710601724 118 41 5.117182910776663 118 42 -0.017748266935054602 118 43 -8.240029159141283 118 44 -6.87822477068676 118 45 -0.8132297285033623 118 46 -7.605728720596446 118 47 -9.832094826300974 118 48 -8.233110704160001 118 49 -10.280473820002852 118 50 -5.358678998021777 118 51 -3.653012096886048 118 52 -3.705427472951662 118 53 -3.834704133457546 118 54 -10.696259008257911 118 55 -3.5103547291512243 118 56 -1.0699440084910492 119 1 0.5406426306594341 119 2 1.7634271999346112 119 3 1.2994073292441186 119 4 1.1374654663391865 119 5 2.5036299220529727 119 6 2.506424450117372 119 7 2.872333985664163 119 8 2.8052678647982034 119 9 2.720813341221948 119 10 3.0365512680106903 119 11 -3.2176491610316695 119 12 -10.72411022380151 119 13 -10.890121644591286 119 14 -9.284279261457733 119 15 -7.161865811290923 119 16 -13.867870569714334 119 17 -14.96538941194256 119 18 -16.001320499404414 119 19 -6.477183533543037 119 20 -8.426328590920818 119 21 -0.09445986135421691 119 22 1.0463588415563123 119 23 1.30205270819296 119 24 1.4261948083592486 119 25 1.4636129888857283 119 26 3.15331269747098 119 27 3.224431893371772 119 28 2.9844572153749453 119 29 3.7149411579365577 119 30 3.672000302645529 119 31 2.516038480681864 119 32 -1.8132178129390262 119 33 2.3844673282464726 119 34 1.884075028507553 119 35 2.2963718453277906 119 36 3.489444491417686 119 37 3.937314029753338 119 38 3.8027652338427522 119 39 4.252036253452765 119 40 8.407752965312575 119 41 2.036611110906616 119 42 -1.5521862136537052 119 43 -12.581322206574681 119 44 -9.27182451077773 119 45 -6.341007506281141 119 46 -14.862876218972014 119 47 -13.435733552746717 119 48 -13.625086012801976 119 49 -12.607796756974912 119 50 -10.121187120179023 119 51 -9.412108913000406 119 52 -8.773491150664466 119 53 -8.83811543950043 119 54 -13.38501794263088 119 55 -9.299016197637254 119 56 -6.769424190427372 120 1 -0.22987476452344424 120 2 0.7634271999346112 120 3 0.41983551836098343 120 4 0.24272862423392638 120 5 1.6144684590734855 120 6 1.7789489817855255 120 7 2.0717095432020756 120 8 1.7486220128802756 120 9 1.7995376944779693 120 10 1.6161676819268394 120 11 -1.7720648612992846 120 12 -9.402522355826488 120 13 -9.30515286671439 120 14 -12.332896567434542 120 15 -9.09005492815087 120 16 -15.789146216458315 120 17 -13.559278796420706 120 18 -16.27429106140263 120 19 -7.432803515701824 120 20 -13.058353568619303 120 21 -4.780231493825227 120 22 -2.36666524408151 120 23 -1.0984825282031174 120 24 -7.90319859039187 120 25 -1.6055217122739514 120 26 2.4468006546520655 120 27 2.0243872903387654 120 28 1.9064019076140184 120 29 1.2838528439312 120 30 2.558708598809666 120 31 1.5956548945980131 120 32 -9.866741452546517 120 33 1.37688481263541 120 34 0.18603756195982157 120 35 0.500653736496389 120 36 1.8112553745577458 120 37 1.8206770984420046 120 38 1.967796455965857 120 39 2.8202788939523202 120 40 5.422694981369666 120 41 -0.9598206464528865 120 42 -4.9281897818963465 120 43 -12.337120600865493 120 44 -11.58025448401591 120 45 -8.40456682831504 120 46 -18.80556132155899 120 47 -16.07712516737651 120 48 -12.959831775513841 120 49 -17.610249923790256 120 50 -12.95838604970623 120 51 -11.816435407202013 120 52 -12.131876520869637 120 53 -12.084547196859932 120 54 -14.54915710409386 120 55 -11.946429221722891 120 56 -8.73954015831319 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 808.0 5 601.0 6 526.0 7 121.0 8 159.0 9 137.0 10 114.0 11 141.0 12 114.0 13 139.0 14 201.0 15 293.0 16 311.0 17 368.0 18 473.0 19 577.0 20 677.0 21 826.0 22 1014.0 23 1237.0 24 1483.0 25 1701.0 26 1821.0 27 2013.0 28 2271.0 29 2791.0 30 3292.0 31 3970.0 32 4388.0 33 5111.0 34 5988.0 35 7149.0 36 9070.0 37 11330.0 38 10575.0 39 6151.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.57137172417714 0.3263032232391564 0.07503837189471889 0.027286680688988686 8 99.39855607981355 0.4843385822295492 0.042066966062190896 0.07503837189471889 9 97.33386390768007 1.7213347734637028 0.5048035927462907 0.4399977261099426 10 41.24154397134899 39.5361264282872 10.322323915638679 8.900005684725143 11 30.69410494002615 34.43920186458985 18.055824000909556 16.81086919447445 12 35.91495651185265 16.733556932522312 21.385935989994884 25.965550565630153 13 24.095152541987424 18.667773444162698 24.623904119713906 32.61316989413597 14 23.691331635437514 21.22862470438421 28.81799163179916 26.262052028379117 15 26.701003908520114 19.375092585206875 27.52600932119375 26.397894185079252 16 29.492343049582452 20.234600769108244 24.998293398866817 25.27476278244249 17 28.267864248763573 22.28639645273151 23.959979534989483 25.485759763515436 18 25.719710751318903 25.093232672366746 23.732263052574133 25.45479352374022 19 26.58583788706739 27.411202185792348 20.958561020036427 25.044398907103826 20 25.2438344315841 27.58714875891973 21.594000022761676 25.575016786734494 21 24.133932124381786 24.305610823716673 25.518731169347962 26.04172588255358 22 25.754078790290492 24.448865897333864 23.80762890114263 25.98942641123302 23 26.756864305610822 25.237905747257123 24.06798931271673 23.93724063441533 24 25.174523046137754 25.86351956704641 25.661141051003934 23.300816335811902 25 26.333682008368203 23.618564671639074 26.607695106421687 23.44005821357104 26 23.076748126499652 22.567292493489656 30.134073256990803 24.22188612301989 27 22.93831741659363 20.773337279318863 30.998372280969345 25.28997302311816 28 21.504776435492502 24.81696972457217 33.10826966650347 20.56998417343186 29 22.66973009247148 22.565088319930844 35.57251561096009 19.19266597663759 30 20.49538279579675 20.389619251239594 40.86680616840286 18.248191784560795 31 20.093038068265827 22.448561777049854 40.465872772147726 16.992527382536597 32 23.49608019388533 18.860582338684903 40.59758553597233 17.04575193145744 33 20.225333500415807 19.716111687039337 43.034369624406196 17.024185188138663 34 16.475527339750155 21.765990852617925 46.83709923316722 14.921382574464696 35 17.10863110474045 21.523725015073435 48.553519220049374 12.814124660136741 36 17.181884354669943 22.54447022428461 46.031800191074105 14.24184522997134 37 17.5949770961052 21.332528088607305 46.833337131657515 14.239157683629983 38 19.46576639255449 21.90426223524984 45.9846938193597 12.645277552835976 39 18.446358581715433 21.463403518366604 44.582307902652445 15.507929997265519 40 13.219049365173946 16.0929550350644 41.39317963056332 29.29481596919833 41 11.89552684124762 15.528677331252439 38.836611507654176 33.73918431984577 42 15.85354743195536 20.567133583874273 46.36032342557795 17.218995558592415 43 12.770903124359062 17.958797657300426 47.43738747977485 21.832911738565667 44 13.28252647935924 18.001393951166005 44.40877046651661 24.307309102958147 45 15.940096420145656 21.000444490033164 47.454439771600505 15.605019318220673 46 15.552822194888616 21.38968611908341 46.10154434877682 16.95594733725115 47 14.383031957238712 21.876492664619583 46.91459115205277 16.825884226088935 48 15.444367247918542 22.92964612353216 47.54496064875454 14.08102597979476 49 13.469602275973172 18.750499868605967 40.993795916501945 26.78610193891891 50 15.640797923213553 21.710616204399507 47.161497472332286 15.487088400054652 51 15.671309764194529 23.701472785972598 45.45672336493378 15.170494084899094 52 14.513540279342962 23.181823355985838 46.45015879520541 15.85447756946579 53 15.836542185969149 22.671967919068557 46.652919866025655 14.838570028936635 54 13.663244493567758 22.48379120566083 43.51819145178382 20.33477284898759 55 14.303630062021162 24.236136446552354 45.12723458591755 16.332998905508937 56 17.65523527401162 24.234932209183093 42.80847670046713 15.301355816338155 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 6.5 5 11.0 6 46.5 7 82.0 8 165.0 9 248.0 10 349.5 11 451.0 12 557.0 13 819.5 14 976.0 15 1154.5 16 1333.0 17 1429.0 18 1525.0 19 1849.5 20 2174.0 21 2613.5 22 3590.0 23 4127.0 24 4498.5 25 4870.0 26 5097.5 27 5325.0 28 5271.5 29 4577.5 30 3937.0 31 3198.5 32 2460.0 33 2078.5 34 1697.0 35 1483.0 36 1269.0 37 1238.0 38 1190.0 39 1173.0 40 1247.5 41 1322.0 42 1669.0 43 2016.0 44 2101.0 45 2186.0 46 2220.0 47 2300.5 48 2347.0 49 2519.0 50 2691.0 51 2893.0 52 3095.0 53 3223.0 54 3641.5 55 3932.0 56 4236.5 57 4541.0 58 4597.0 59 4653.0 60 4283.0 61 3913.0 62 3458.5 63 2563.0 64 2122.0 65 1824.5 66 1527.0 67 1334.0 68 1141.0 69 994.0 70 847.0 71 718.5 72 461.0 73 332.0 74 249.5 75 167.0 76 120.5 77 74.0 78 52.5 79 20.5 80 10.0 81 8.5 82 7.0 83 4.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.013643340344494343 14 0.0034108350861235858 15 0.22511511568415668 16 0.06935364675117958 17 0.0 18 0.0034108350861235858 19 0.13074867830140413 20 0.10005116252629187 21 0.0 22 0.0 23 0.0 24 0.001136945028707862 25 0.0034108350861235858 26 0.020465010516741516 27 0.11596839292820194 28 0.14552896367460633 29 0.04093002103348303 30 0.026149735660280823 31 0.03865613097606731 32 0.07731226195213461 33 0.197828434995168 34 0.06935364675117958 35 0.059121141492808824 36 0.035245295889943726 37 0.22397817065544884 38 0.3160707179807856 39 0.2126087203683702 40 0.7810812347223012 41 0.9243363083394919 42 0.16485702916264 43 0.21943039054061736 44 0.49343414245921213 45 0.24330623614348246 46 0.17167869933488716 47 0.02842362571769655 48 0.17736342447842648 49 0.4911602524017964 50 0.14439201864589848 51 0.3388096185549429 52 0.12051617304303337 53 0.20123927008129155 54 0.22056733556932523 55 0.27627764197601046 56 0.21033483031095446 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 87955.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.07321925984878 #Duplication Level Percentage of deduplicated Percentage of total 1 85.9414437152953 61.94076516400432 2 7.955262493690056 11.467227559547496 3 2.5744573447753663 5.566482860553693 4 1.2130868248359414 3.4972429083053833 5 0.6420368500757193 2.313683133420499 6 0.41172387682988393 1.7804559149565118 7 0.2681726400807673 1.3529645841623557 8 0.20665068147400303 1.1915183900858395 9 0.1561711256940939 1.013018020578705 >10 0.5978672387682988 7.248024558012619 >50 0.020507319535588087 1.0596327667557275 >100 0.012619888944977283 1.5689841396168496 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 448 0.5093513728611222 No Hit ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 172 0.19555454493775226 No Hit ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 142 0.16144619407651642 No Hit ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 140 0.15917230401910068 No Hit ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 132 0.15007674378943778 No Hit ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 128 0.14552896367460633 No Hit ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 111 0.12620089818657268 No Hit ACAATAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 107 0.12165311807174123 No Hit ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 93 0.10573588766983116 No Hit ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 93 0.10573588766983116 No Hit ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 92 0.1045989426411233 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.013643340344494343 0.0 0.0 0.0 0.0 7 0.013643340344494343 0.0 0.0 0.0 0.0 8 0.013643340344494343 0.0 0.0 0.0 0.0 9 0.013643340344494343 0.0 0.0 0.0 0.0 10 0.02160195554544938 0.0 0.0 0.0 0.0 11 0.03524529588994372 0.0 0.0 0.0 0.0 12 0.04547780114831448 0.0 0.0 0.0 0.0 13 0.0477516912057302 0.0 0.0 0.0 0.0 14 0.052299471320561654 0.0 0.0 0.0 0.0 15 0.055710306406685235 0.0 0.0 0.0 0.0 16 0.06366892160764027 0.0 0.0 0.0 0.0 17 0.06707975669376386 0.0 0.0 0.0 0.0 18 0.07617531692342676 0.0 0.0 0.0 0.0 19 0.08299698709567392 0.0 0.0 0.0 0.0 20 0.09095560229662895 0.0 0.0 0.0 0.0 21 0.10118810755499971 0.0 0.0 0.0 0.0 22 0.11028366778466261 0.0 0.0 0.0 0.0 23 0.1284747882439884 0.0 0.0 0.0 0.0 24 0.13529645841623558 0.0 0.0 0.0 0.0 25 0.14552896367460633 0.0 0.0 0.0 0.0 26 0.1580353589903928 0.0 0.0 0.0 0.0 27 0.16826786424876358 0.0 0.0 0.0 0.0 28 0.17850036950713433 0.0 0.0 0.0 0.0 29 0.1853220396793815 0.0 0.0 0.0 0.0 30 0.18759592973679723 0.0 0.0 0.0 0.0 31 0.1944175999090444 0.0 0.0 0.0 0.0 32 0.19782843499516797 0.0 0.0 0.0 0.0 33 0.2001023250525837 0.0 0.0 0.0 0.0 34 0.20237621510999942 0.0 0.0 0.0 0.0 35 0.20237621510999942 0.0 0.0 0.0 0.0 36 0.20465010516741516 0.0 0.0 0.0 0.0 37 0.205787050196123 0.0 0.0 0.0 0.0 38 0.205787050196123 0.0 0.0 0.0 0.0 39 0.205787050196123 0.0 0.0 0.0 0.0 40 0.205787050196123 0.0 0.0 0.0 0.0 41 0.20806094025353875 0.0 0.0 0.0 0.0 42 0.21715650048320165 0.0 0.0 0.0 0.0 43 0.2239781706554488 0.0 0.0 0.0 0.0 44 0.22625206071286452 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCCTC 15 0.008058495 50.53522 38 ACATAGA 15 0.008076917 50.50606 40 GCCGCAT 20 3.960585E-4 50.506058 39 AAGACTA 15 0.008244129 50.24512 36 ATGCTCG 15 0.008244129 50.24512 36 TTAGAGC 15 0.008262866 50.216293 35 ATAGAAT 15 0.008262866 50.216293 42 CGTTGTG 15 0.008300435 50.15874 34 GTTAGAG 15 0.008300435 50.15874 34 ACGATTC 15 0.008319266 50.130013 48 ACCAAGC 15 0.008319266 50.130013 48 TTACCGA 15 0.008319266 50.130013 49 ACGATTA 25 2.0399368E-5 50.13001 48 AGCAAGG 15 0.00833813 50.10132 45 TACCGAA 15 0.008394912 50.01543 50 ATGCTAG 15 0.008413903 49.986866 15 ATTCAAG 15 0.008432926 49.958336 33 TCCCAAT 15 0.008432926 49.958336 33 TCGTTGT 20 4.1797527E-4 49.958332 33 TTCGTTG 20 4.1797527E-4 49.958332 32 >>END_MODULE