FastQCFastQC Report
Fri 17 Jun 2016
SRR1526228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63994
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4170.6516235897115354No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1670.26096196518423603No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1220.19064287276932212No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1160.18126699378066694No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1140.17814170078444855No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1010.15782729630902897No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC990.1547020033128106No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG980.1531393568147014No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG910.14220083132793698No Hit
AGCGAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG910.14220083132793698No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.1390755383317186No Hit
AGCGAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG810.12657436634684502No Hit
AGCGAGGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG760.11876113385629902No Hit
AGCGAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA690.10782260836953465No Hit
AGCGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT670.10469731537331625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTC150.00823323350.24605640
CGATTAA204.0544177E-450.24605649
ACGATTA204.0544177E-450.24605648
CAACGAT204.0702432E-450.20646346
AACGATT204.0702432E-450.20646347
GCTACTT150.008258956550.20646346
TCAACGA204.0702432E-450.20646345
TAGAAGG150.0082847450.16692715
ATGGTTC204.0861178E-450.16692714
TGGGATC150.0082847450.16692715
AAATGAG150.0082847450.16692715
ACTACCT150.008310582550.1274613
CCGACTC150.008310582550.1274643
GCAGCTA150.008310582550.1274643
AGACTAC150.00836244950.04870211
ATATGCC252.0532589E-550.04870234
AGAGTTA150.00836244950.04870210
AAACATG150.00836244950.04870210
GATTGTA150.00836244950.04870210
GAGTTAT150.00836244950.04870211