##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526228_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	63994
Sequences flagged as poor quality	0
Sequence length	56
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.32293652529925	39.0	38.0	39.0	33.0	40.0
2	35.941525768040755	38.0	35.0	39.0	28.0	40.0
3	36.13493452511173	39.0	36.0	39.0	29.0	40.0
4	35.91296059005532	38.0	35.0	39.0	29.0	40.0
5	36.249601525142985	38.0	36.0	39.0	31.0	40.0
6	36.01121980185643	39.0	36.0	39.0	30.0	40.0
7	36.5643810357221	39.0	37.0	39.0	32.0	40.0
8	36.445401131356064	39.0	37.0	39.0	31.0	40.0
9	35.73906928774573	39.0	35.0	39.0	29.0	40.0
10	35.11483889114604	39.0	35.0	39.0	27.0	40.0
11	34.938978654248835	38.0	34.0	39.0	28.0	40.0
12	33.280448167015656	37.0	32.0	39.0	18.0	40.0
13	33.33728162015189	38.0	32.0	39.0	18.0	40.0
14	33.51143857236616	38.0	32.0	39.0	19.0	40.0
15	33.46612182392099	38.0	32.0	39.0	19.0	40.0
16	32.88508297652905	37.0	31.0	39.0	18.0	40.0
17	32.971825483639094	36.0	31.0	39.0	20.0	40.0
18	32.50996968465794	36.0	30.0	39.0	18.0	40.0
19	33.27532268650186	36.0	31.0	39.0	23.0	40.0
20	33.08919586211207	36.0	31.0	39.0	22.0	40.0
21	33.85362690252211	38.0	33.0	39.0	23.0	40.0
22	33.63863799731225	37.0	33.0	39.0	23.0	40.0
23	33.87994186955027	38.0	33.0	39.0	23.0	40.0
24	33.58447666968778	37.0	32.0	39.0	23.0	40.0
25	33.660030627871365	37.0	32.0	39.0	23.0	40.0
26	34.23311560458793	38.0	33.0	39.0	25.0	40.0
27	34.11391692971216	38.0	33.0	39.0	24.0	40.0
28	33.97688845829296	38.0	33.0	39.0	24.0	40.0
29	33.87517579773104	38.0	33.0	39.0	23.0	40.0
30	33.60960402537738	37.0	33.0	39.0	23.0	40.0
31	33.80012188642685	37.0	33.0	39.0	24.0	40.0
32	33.46135575210176	37.0	33.0	39.0	23.0	40.0
33	33.61399506203706	37.0	33.0	39.0	23.0	40.0
34	33.3496734068819	37.0	33.0	39.0	23.0	40.0
35	33.20945713660656	37.0	33.0	39.0	21.0	40.0
36	32.35050160952589	35.0	31.0	38.0	19.0	39.0
37	32.11029158983655	35.0	31.0	38.0	18.0	39.0
38	31.848876457167858	35.0	31.0	38.0	16.0	39.0
39	31.564209144607307	35.0	30.0	38.0	11.0	39.0
40	28.184048504547302	34.0	20.0	38.0	4.0	39.0
41	25.04192580554427	30.0	12.0	36.0	4.0	39.0
42	28.818904897334125	33.0	26.0	37.0	4.0	39.0
43	27.3986780010626	32.0	22.0	36.0	4.0	39.0
44	27.46004312904335	32.0	22.0	37.0	4.0	39.0
45	28.4622308341407	33.0	26.0	37.0	4.0	39.0
46	27.98440478794887	33.0	23.0	38.0	4.0	39.0
47	27.57442885270494	33.0	23.0	38.0	4.0	39.0
48	27.611713598149827	33.0	23.0	38.0	4.0	39.0
49	25.273447510704127	31.0	10.0	37.0	4.0	39.0
50	27.224021001968936	33.0	22.0	37.0	4.0	39.0
51	27.53003406569366	33.0	21.0	38.0	4.0	39.0
52	27.242788386411227	33.0	18.0	38.0	4.0	39.0
53	26.850970403475326	33.0	15.0	38.0	4.0	39.0
54	25.9882645247992	33.0	6.0	38.0	4.0	39.0
55	26.250617245366755	33.0	4.0	38.0	4.0	39.0
56	25.563052786198707	32.0	4.0	38.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.5001462580006901
1	2	3.0103648283191333
1	3	3.03024641548771
1	4	2.942780266621355
1	5	1.4803822347556803
1	6	2.7155439890512554
1	7	2.438570333279756
1	8	2.404566053222645
1	9	2.5704015867284795
1	10	2.6712301992404903
1	11	-2.6869083043619106
1	12	-12.971451623562427
1	13	-7.125245174260616
1	14	-4.516477355028435
1	15	-5.67220920638545
1	16	-21.766102168340044
1	17	-3.727726544798532
1	18	-11.87020750750952
1	19	-4.4269122043986044
1	20	-4.2003504456424245
1	21	-4.454688321323179
1	22	-1.6488400044019045
1	23	-1.619039045062813
1	24	-2.7373480625023348
1	25	-1.901419974667565
1	26	3.762913372680991
1	27	3.697601797354146
1	28	2.817741271251215
1	29	3.6801320740822376
1	30	3.958901022220722
1	31	2.936332348504365
1	32	2.1111450611821496
1	33	2.795714945236064
1	34	3.288835844807366
1	35	3.438158236723922
1	36	3.5863229992732535
1	37	3.816986843771822
1	38	3.2326010747261726
1	39	2.96752440998743
1	40	5.229404013545896
1	41	-11.827049996701472
1	42	-12.718408369752618
1	43	-18.341027763631295
1	44	-20.676236968442772
1	45	-15.309824343702779
1	46	-17.93399924557491
1	47	-21.24561316869945
1	48	-16.947654378299745
1	49	-18.77809278195595
1	50	-15.709909098449447
1	51	-13.990119193307237
1	52	-13.97199482102753
1	53	-13.365107117295482
1	54	-12.825523180679209
1	55	-12.962269072787612
1	56	-10.820070655769976
2	1	2.102368480222914
2	2	3.670364828319137
2	3	3.6458019710432623
2	4	3.3738913777324626
2	5	2.164826679200125
2	6	3.3844328779401422
2	7	2.847459222168645
2	8	3.1623438310004204
2	9	3.679290475617364
2	10	3.217896865907157
2	11	1.7575361400825358
2	12	-2.562562734673538
2	13	-0.5808007298161719
2	14	3.29907820052712
2	15	3.5277907936145496
2	16	2.856120053882176
2	17	4.234495677423688
2	18	-0.5768741741761865
2	19	2.661976684490284
2	20	3.481871776579794
2	21	3.0408672342323797
2	22	3.2133822178203175
2	23	3.3942942882705225
2	24	1.682651937497667
2	25	2.7874689142213214
2	26	2.8118022615698806
2	27	2.9287129084652506
2	28	3.4955190490289922
2	29	3.49346540741557
2	30	3.363345466665166
2	31	3.1274434596154777
2	32	4.06225617229326
2	33	2.9401593896805096
2	34	2.9088358448073635
2	35	2.8692693478350364
2	36	3.292989665939917
2	37	3.4992090659940445
2	38	2.6814899636150606
2	39	3.594191076654102
2	40	5.791626235768117
2	41	-4.382605552257026
2	42	-5.2606305919748415
2	43	-10.345472208075737
2	44	-12.83623696844277
2	45	-4.965379899258334
2	46	-10.251777023352687
2	47	-17.441168724255007
2	48	-8.414321044966414
2	49	-12.480315004178175
2	50	-7.736575765116115
2	51	-3.0878969710850157
2	52	-4.045328154360863
2	53	-4.0339960061843705
2	54	-3.3921898473458754
2	55	-3.0067135172320576
2	56	-0.8934039891033088
3	1	2.2716356197028134
3	2	4.101570502078005
3	3	3.9398918055586307
3	4	3.5741277843518446
3	5	2.6884673411386615
3	6	3.876395052881037
3	7	3.1089249432088337
3	8	3.4796015142155525
3	9	3.913096622189471
3	10	3.8795989935667308
3	11	3.836495950957236
3	12	5.270534192040415
3	13	3.7598612087181067
3	14	4.66763612014887
3	15	4.393039020564906
3	16	5.43106095222733
3	17	5.708727355910689
3	18	6.120998166249347
3	19	5.1433005615588385
3	20	4.875252391237005
3	21	4.140630827612995
3	22	4.128748648080368
3	23	4.2106063450081095
3	24	4.545772504873554
3	25	4.480140309020378
3	26	3.874686422326377
3	27	3.8019989604747124
3	28	3.985826377634197
3	29	3.6097774641531615
3	30	4.072092511582426
3	31	3.380871355596568
3	32	3.088024493806259
3	33	3.1028071438176283
3	34	3.0208925823960158
3	35	3.231207882113992
3	36	4.03057831842219
3	37	4.250745709020052
3	38	3.945083344229719
3	39	4.538162707859772
3	40	8.099900467446606
3	41	8.270822343724063
3	42	5.5452795735097915
3	43	0.8670573427516857
3	44	4.005607003188434
3	45	5.4029416137440265
3	46	4.1749369246378585
3	47	-1.7785209701178921
3	48	4.2580193805655
3	49	5.771694452086599
3	50	4.129381681692397
3	51	5.804065203855881
3	52	5.6979342569866525
3	53	5.511630471357002
3	54	6.246108024994552
3	55	5.2923408562904015
3	56	5.715815869052719
4	1	1.939696819798442
4	2	3.078979060528873
4	3	3.450171509120665
4	4	3.542930079355436
4	5	2.5068990886882645
4	6	3.251723764332148
4	7	2.368308160995113
4	8	3.0448656786908046
4	9	3.2327236841067517
4	10	3.613402483884684
4	11	3.427698437211127
4	12	5.363529649845816
4	13	4.641046960570847
4	14	4.755133131862948
4	15	3.4229218797568706
4	16	4.884085097577557
4	17	5.4575918072613945
4	18	5.609642679756394
4	19	4.265222627062066
4	20	4.754406108664689
4	21	3.9695813415981718
4	22	4.084043890729184
4	23	3.833245599131942
4	24	4.828344821392797
4	25	4.65513433244854
4	26	3.553624983167879
4	27	3.62509243405826
4	28	3.48965138361077
4	29	3.0807313250185686
4	30	3.5863167525578064
4	31	3.352661936519347
4	32	3.372867907624098
4	33	2.363505207408352
4	34	2.441045582635077
4	35	2.4899934427164325
4	36	4.480255583542917
4	37	3.5058257950826857
4	38	4.193350138396585
4	39	3.9320187920099023
4	40	8.412475174594586
4	41	10.44486011565808
4	42	6.956348184554496
4	43	7.8989722363687065
4	44	7.371253668261346
4	45	6.2632093641623925
4	46	6.992292889256554
4	47	5.032738891225641
4	48	6.07107221346055
4	49	9.616064521414831
4	50	6.807244459602991
4	51	5.656922005194634
4	52	5.696619411182205
4	53	6.164630710419875
4	54	6.321293298721539
4	55	5.186794597624374
4	56	6.448543576439761
5	1	2.1596917125461417
5	2	3.4627890707433764
5	3	3.5910039912452802
5	4	3.886265115106198
5	5	2.629473143846589
5	6	3.547816716323979
5	7	3.014630939340364
5	8	3.481535750192343
5	9	3.730553101879991
5	10	3.6428968659071543
5	11	4.033546241092637
5	12	6.1402150431042415
5	13	5.333845734830291
5	14	5.869280220729138
5	15	5.4974877633115184
5	16	5.821776619538742
5	17	5.4925764855045
5	18	6.823125825823812
5	19	4.882178704692301
5	20	5.078588948296968
5	21	4.226220769585915
5	22	3.9631296925677972
5	23	4.498082167058399
5	24	4.420530725376452
5	25	4.826610328362733
5	26	3.4600345848022016
5	27	3.8372987670511094
5	28	3.631377634887585
5	29	4.169980558930725
5	30	3.6325373858570913
5	31	3.1057262878983067
5	32	2.883720818757908
5	33	3.5691997937209123
5	34	3.269441905413423
5	35	2.7839158124814958
5	36	4.649050272000526
5	37	4.626229268014249
5	38	4.23957077169587
5	39	5.038888046351065
5	40	8.808797952939837
5	41	10.882040912389435
5	42	6.291894660550415
5	43	8.72851769091416
5	44	8.43573272852693
5	45	7.190175656297221
5	46	8.423728027152364
5	47	7.585598952512669
5	48	8.28340622776086
5	49	10.737361763498594
5	50	8.31796968942934
5	51	6.88457777638973
5	52	7.284974875942169
5	53	6.810953488765126
5	54	7.937355607199578
5	55	7.589246078727541
5	56	6.275535404836084
6	1	1.8560083269662044
6	2	2.9243878168248827
6	3	3.263809633878509
6	4	3.3179526804144572
6	5	1.7318764876292434
6	6	2.4829003108903294
6	7	2.618340448222284
6	8	2.4454855934525312
6	9	2.584654460291695
6	10	1.983414107286464
6	11	2.5153905462127994
6	12	4.96923803160999
6	13	4.375904251026739
6	14	4.687200805891102
6	15	5.205951713154782
6	16	5.643093233958805
6	17	5.263997593132501
6	18	5.351861458007722
6	19	4.260444117440471
6	20	4.743557600334583
6	21	3.017495586722795
6	22	2.8279416047935015
6	23	2.64946670206362
6	24	3.578054236348237
6	25	2.7261662322289837
6	26	2.3183156715315647
6	27	1.3072569697679342
6	28	1.9717642597569665
6	29	2.169327476381092
6	30	2.024648148657505
6	31	2.945067980688272
6	32	2.095972647389047
6	33	2.0669793130521583
6	34	2.978950787336096
6	35	1.4064340987928858
6	36	3.292989665939917
6	37	1.9562971885994074
6	38	2.5857045230020326
6	39	3.4700531456196195
6	40	6.490093668718313
6	41	3.949961497551403
6	42	5.359292779672668
6	43	6.5886274087825
6	44	5.524682571787114
6	45	5.356842322963885
6	46	5.290368570517046
6	47	4.702892578426987
6	48	5.04085136882669
6	49	6.355700321492325
6	50	4.449631131435609
6	51	5.174938277957132
6	52	5.404097132995457
6	53	4.610065296497623
6	54	5.291718198631138
6	55	4.529684950200892
6	56	6.489354631586345
7	1	1.3801462580006856
7	2	2.978698161652467
7	3	3.044413082154371
7	4	2.916946933288017
7	5	2.182882234755681
7	6	2.583043989051255
7	7	2.291903666613088
7	8	2.1553993865559775
7	9	2.6487349200618127
7	10	2.842896865907157
7	11	2.6630916956380872
7	12	4.465215043104244
7	13	4.449754825739383
7	14	4.67268931163823
7	15	4.611124126947885
7	16	4.792231164993286
7	17	4.841440121868132
7	18	4.960625825823815
7	19	3.910587795601394
7	20	4.551316221024244
7	21	3.5353116786768197
7	22	2.9744933289314304
7	23	3.615127621603854
7	24	2.9784852708310012
7	25	3.3402466919990985
7	26	2.5770800393476563
7	27	2.663435130687475
7	28	1.9677412712512208
7	29	2.5517987407489073
7	30	2.3314010222207244
7	31	2.1954990151710305
7	32	3.505311727848813
7	33	2.7123816119027353
7	34	2.0921691781406935
7	35	2.5373249033905907
7	36	4.1388229992732555
7	37	3.5103201771051573
7	38	4.100934408059505
7	39	4.0650244099874335
7	40	5.923570680212567
7	41	2.047950003298528
7	42	4.84075829691405
7	43	7.2739722363687065
7	44	6.922096364890564
7	45	5.65267565629722
7	46	5.338500754425091
7	47	5.293553497967217
7	48	5.010678955033587
7	49	6.604407218044049
7	50	4.8509242348838875
7	51	4.335714140026095
7	52	5.4088385123058025
7	53	5.488226216037852
7	54	6.257810152654123
7	55	5.433564260545722
7	56	5.598262677563358
8	1	1.7599215388995617
8	2	3.067743105472694
8	3	3.5625310596824633
8	4	3.4867503040745333
8	5	1.9788092010478167
8	6	3.577566460961364
8	7	2.7952744531299416
8	8	2.763966802286312
8	9	3.5360944706236097
8	10	3.7819418097273783
8	11	3.1692714709189858
8	12	5.779259986924465
8	13	5.270260443716914
8	14	5.541649985795537
8	15	4.917303902228781
8	16	5.468354760498908
8	17	5.558715402767007
8	18	5.800653915711454
8	19	4.141627121444088
8	20	5.069012850237726
8	21	4.115648757328508
8	22	3.780673104212326
8	23	3.4624590822780092
8	24	4.075535832628752
8	25	3.4416511863811223
8	26	2.9244115000218116
8	27	2.3441935576537674
8	28	2.2986401476557106
8	29	2.5526414373781208
8	30	2.485193157052187
8	31	1.981875419665414
8	32	2.47399150312971
8	33	2.38597711752071
8	34	2.4747534478036144
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115	29	3.192900435664157
115	30	3.1629688188308904
115	31	3.286388845679511
115	32	1.9432354566623715
115	33	2.516618900038324
115	34	4.287931890005105
115	35	4.16719778474652
115	36	4.050051812832574
115	37	2.5713371262576956
115	38	3.0581377978900086
115	39	3.239388816767093
115	40	7.044545256483747
115	41	6.633966952451072
115	42	2.1062243986089655
115	43	-5.643400644987224
115	44	-5.743581601211133
115	45	1.774921419009086
115	46	-4.668914499812196
115	47	-3.5003024342361755
115	48	-4.410931214457939
115	49	-14.769957188735614
115	50	-4.221321527827978
115	51	-2.2750909447196648
115	52	-2.50239030125352
115	53	-3.2541466653180784
115	54	-5.349816965989945
115	55	-1.4662238750475005
115	56	1.419025389427766
116	1	1.648639408685618
116	2	3.1941091205565826
116	3	2.851433630099578
116	4	3.2883510428770606
116	5	1.9806562073584217
116	6	2.8393796054896114
116	7	2.5603968172980203
116	8	2.0562555509395395
116	9	2.642913002253593
116	10	2.4973489207016755
116	11	-1.9006069344988958
116	12	-11.861497285662882
116	13	-7.927984900288013
116	14	-2.961043565074096
116	15	-6.440245736065812
116	16	-2.97523458843137
116	17	-2.073114672652416
116	18	-8.642627598833723
116	19	-2.2186930263164157
116	20	-2.8147796693867164
116	21	1.9606541444302437
116	22	3.094356342630057
116	23	2.2865317311928948
116	24	2.469409928365245
116	25	1.866616555012797
116	26	2.3512238749640915
116	27	2.868743349865561
116	28	2.8944536000183447
116	29	2.993922028420137
116	30	3.231229789344013
116	31	3.137793535718977
116	32	2.69178433058854
116	33	2.4267651735465705
116	34	3.281895205537957
116	35	2.528934492431688
116	36	3.6126243691362667
116	37	4.156895519570909
116	38	3.9341535861416936
116	39	4.856291533275101
116	40	8.536070680212564
116	41	5.507196578640993
116	42	1.0446966530784323
116	43	-6.03253461294636
116	44	-6.015232402232726
116	45	1.3203126425985907
116	46	-2.439752670232444
116	47	-1.3175766390190873
116	48	-2.3951429627746315
116	49	-5.688229768257322
116	50	-1.7804113815544689
116	51	0.5273237290671915
116	52	-0.3663327205709095
116	53	-0.9583491264279012
116	54	-7.508628203510259
116	55	-0.11934669835838818
116	56	0.9883311707140443
117	1	0.9526952776085338
117	2	2.244874632240702
117	3	1.9784817096053544
117	4	2.4667018352488057
117	5	1.0929312543635277
117	6	2.058289087090465
117	7	1.1193546470052453
117	8	1.9904484061638215
117	9	-0.7135199818989761
117	10	-0.295828624288923
117	11	-5.102594578871713
117	12	-11.180863388268307
117	13	-11.129166742888067
117	14	-14.642751864832356
117	15	-11.511424892659956
117	16	-16.770023736967495
117	17	-13.777922623229905
117	18	-14.412168291823246
117	19	-6.7131867142025285
117	20	-7.930546524073797
117	21	-0.5362569487741595
117	22	-0.6284478475391566
117	23	-1.0645292411412441
117	24	-1.3820539448552758
117	25	-1.0425964452558034
117	26	1.3582074903280486
117	27	1.284268464020812
117	28	0.2726432320355343
117	29	1.2464065838861629
117	30	0.9306657281030795
117	31	0.6320186230141687
117	32	0.4178117278488145
117	33	0.7133620040595972
117	34	0.699031923238735
117	35	0.8601190210376473
117	36	1.3322053522144266
117	37	1.8318888045561366
117	38	1.3220128394320518
117	39	1.6843871550854708
117	40	5.7909726409968805
117	41	2.4239303954553897
117	42	-1.6697809187722257
117	43	-10.414753253827373
117	44	-10.263687948834928
117	45	-1.4666870888008177
117	46	-4.405371794594515
117	47	-5.324828854973962
117	48	-5.065301437123274
117	49	-9.52868101725007
117	50	-5.56794831413572
117	51	-5.494432918797433
117	52	-5.961014428870669
117	53	-6.339224764354306
117	54	-8.245131023816462
117	55	-5.836778876709182
117	56	-3.895364773417036
118	1	1.1590936264217433
118	2	1.7598385125296616
118	3	2.0393692225052504
118	4	2.1189206174985458
118	5	1.0888032873872646
118	6	2.125368550454759
118	7	1.7550615613499332
118	8	1.793337983047202
118	9	1.3711033411144413
118	10	1.4564933571352228
118	11	-3.6237504096250674
118	12	-10.49355688672032
118	13	-11.500683770751845
118	14	-10.289810688361769
118	15	-5.577472364280183
118	16	-12.334523220971622
118	17	-9.83158619392134
118	18	-12.878628560141099
118	19	-5.588315713170537
118	20	-6.036841673712601
118	21	1.3502239593785745
118	22	2.110458241212129
118	23	2.2223644637091127
118	24	1.1370379024099435
118	25	1.5301589727008533
118	26	2.456246706014319
118	27	1.7404088148980037
118	28	2.0001974116020946
118	29	2.0338162846085552
118	30	2.2206554081856353
118	31	0.39878848885524576
118	32	-3.9506093247827643
118	33	0.5079956469904516
118	34	0.9281340904213948
118	35	0.4493863068993633
118	36	3.1000072097995677
118	37	3.612074563070067
118	38	3.21365370630512
118	39	3.808226164373398
118	40	6.834316294247653
118	41	1.5694412313687032
118	42	-3.361215387296479
118	43	-10.118571623280417
118	44	-7.583605389495403
118	45	-3.06421030861506
118	46	-10.026630824522279
118	47	-11.759297379225767
118	48	-10.298531571282204
118	49	-12.058092781955953
118	50	-8.399733659852956
118	51	-6.008364807342325
118	52	-6.078661487694198
118	53	-5.553879047120041
118	54	-9.54131265436342
118	55	-5.228935739454279
118	56	-3.554807497875238
119	1	1.316988363263846
119	2	2.970364828319134
119	3	2.7937551874175313
119	4	3.0487451789020525
119	5	2.1414348663346274
119	6	2.721859778524937
119	7	2.1410264736306317
119	8	2.3021099128717637
119	9	1.7746121130442702
119	10	2.052984585205401
119	11	-5.307960935940859
119	12	-9.37074986917646
119	13	-11.342789033909739
119	14	-13.412617705905628
119	15	-10.068700434455625
119	16	-12.983646027989167
119	17	-16.656147597430113
119	18	-16.106698735579698
119	19	-6.5356841342231675
119	20	-7.931578515817865
119	21	-0.0883725318494939
119	22	1.6894056096331838
119	23	1.4679784987968318
119	24	1.1721256217081901
119	25	1.6354221305955932
119	26	2.508878284961689
119	27	3.1965491657751954
119	28	3.070372850198588
119	29	2.4022373372401304
119	30	3.4662694432733545
119	31	2.3812446292061225
119	32	-0.7751707282915348
119	33	2.700978103130801
119	34	3.173748125509114
119	35	2.9757020963730483
119	36	3.714042297518869
119	37	3.7524254402630532
119	38	3.4241800220945926
119	39	4.281910374899713
119	40	7.658877697756427
119	41	0.797511406807299
119	42	-3.080513632910513
119	43	-9.767694430297961
119	44	-8.653780828091893
119	45	-6.379999782299269
119	46	-15.254700999960875
119	47	-13.40842018624331
119	48	-13.175724553738343
119	49	-11.60195243107876
119	50	-10.820786291431903
119	51	-8.762750772254606
119	52	-8.201468505238058
119	53	-9.360896590979692
119	54	-9.067628443837103
119	55	-7.5798129324367345
119	56	-5.730246094366468
120	1	0.7508779653177626
120	2	2.157356698237834
120	3	2.130083813861688
120	4	2.1831054698733823
120	5	0.9398944298776328
120	6	2.463836671978079
120	7	1.8333670812472391
120	8	1.5691188987510998
120	9	1.4725154078666876
120	10	1.5057017439559317
120	11	-3.1064204994838605
120	12	-10.789663005676246
120	13	-8.325245174260615
120	14	-13.753225322508111
120	15	-11.407168555978945
120	16	-16.340085908177443
120	17	-16.30398670740016
120	18	-15.396386369298138
120	19	-7.8317902531790935
120	20	-12.139537437512345
120	21	-5.029322467664642
120	22	-3.1779456954588134
120	23	-0.891884573518098
120	24	-8.162063509656807
120	25	-0.849387454342363
120	26	2.4481166247135064
120	27	2.341179033126501
120	28	2.871399807836582
120	29	1.3731402041635405
120	30	2.592071753928039
120	31	1.2293404785856694
120	32	-11.996822418492648
120	33	1.481893807024683
120	34	1.5909496659455726
120	35	0.9906785619271758
120	36	2.8702254382976413
120	37	1.9591006649100322
120	38	1.6381295300107261
120	39	2.064109775841093
120	40	5.560460924115002
120	41	0.4119743935424296
120	42	-5.010766093329853
120	43	-10.369320446558122
120	44	-6.49802558632895
120	45	-7.870799953458878
120	46	-15.047169977282225
120	47	-13.71650747764254
120	48	-13.053345435210316
120	49	-16.472726928297416
120	50	-11.795112350481967
120	51	-10.69385903070561
120	52	-10.664027341352735
120	53	-9.662993296157271
120	54	-12.355604481492216
120	55	-10.594789397990864
120	56	-8.838932444387861
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1310.0
5	103.0
6	56.0
7	73.0
8	185.0
9	184.0
10	149.0
11	150.0
12	155.0
13	164.0
14	200.0
15	285.0
16	299.0
17	349.0
18	406.0
19	525.0
20	607.0
21	772.0
22	919.0
23	1070.0
24	1230.0
25	1320.0
26	1492.0
27	1648.0
28	1818.0
29	2091.0
30	2617.0
31	2938.0
32	3452.0
33	3903.0
34	4564.0
35	5471.0
36	6598.0
37	7420.0
38	6256.0
39	3214.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.14992030502859	0.718817389130231	0.09219614338844266	0.039066162452729945
8	99.17336000250023	0.695377691658593	0.08594555739600589	0.045316748445166734
9	97.38569240866332	1.5954620745694907	0.5703659718098572	0.4484795449573397
10	51.390755383317185	31.776416539050533	9.435259555583336	7.3975685220489416
11	37.89574022564615	31.224802325217986	15.723349063974748	15.15610838516111
12	36.40028752695565	14.43260305653655	21.923930368472043	27.243179048035753
13	24.530307293132015	15.327143705648815	26.005814498733944	34.136734502485226
14	23.29161001984592	18.81612051318113	30.95963621021049	26.932633256762458
15	28.84061149050811	18.03771693502913	27.399599022617632	25.72207255184512
16	32.70484498850898	19.33617872832731	24.084236199052576	23.874740084111128
17	29.221489514641995	20.74256961590149	24.828890208457043	25.20705066099947
18	27.408113514189274	22.36842105263158	24.456182022752845	25.767283410426305
19	29.40955599361682	24.58618855408492	21.732532307018367	24.27172314527989
20	29.087297863688967	25.94538800788214	21.702480372837883	23.264833755591006
21	28.57299121792668	22.47554458230459	26.105572397412256	22.845891802356473
22	26.87126918148576	21.586398724880457	25.71491077288496	25.827421320748822
23	27.40725693033722	21.59733725036722	25.73366253086227	25.26174328843329
24	27.91667968872082	22.325530518486108	26.982217082851516	22.775572709941557
25	30.48662332791599	20.507250906363296	25.056257032129015	23.949868733591696
26	24.24981245311328	19.861215303825958	30.263815953988498	25.625156289072272
27	23.654113929001674	17.776178481468154	30.478589420654913	28.091118168875262
28	23.428428350104067	22.215614779111437	31.447082205286303	22.908874665498193
29	28.118796404845643	18.652598671356	31.221570926143023	22.007033997655334
30	23.904345107846204	17.844638949671772	37.23038449515473	21.02063144732729
31	24.34147790335944	19.550094577060765	35.38745329769107	20.720974221888728
32	28.33682630047859	16.772498357784105	35.09024367355876	19.800431668178547
33	26.226223091976518	16.778082191780822	37.12093933463797	19.874755381604697
34	22.18400687876182	18.360040647228953	42.66083014148362	16.7951223325256
35	21.357659897128027	19.623845035411097	43.96916967621907	15.04932539124181
36	21.693866066404052	22.48796348402426	39.27030575876947	16.547864690802225
37	22.2439711869715	19.310992796742877	40.974005637331665	17.471030378953962
38	23.535035340950053	21.01336843919946	39.02079709122824	16.43079912862225
39	23.91321363159625	18.06797013196412	38.0253909613187	19.993425275120927
40	17.34281354705762	14.247062339746112	36.14069308332415	32.26943102987211
41	14.954935081305937	14.587797806630531	34.276125047270895	36.18114206479264
42	20.09949626106818	18.632082850974626	40.15362473013986	21.114796157817338
43	16.864544828018293	14.809535743374475	41.27560929766305	27.050310130944176
44	17.13891025540398	15.192377125096149	39.051536034409686	28.61717658509018
45	22.00056371551157	17.716325827565687	41.569321349159125	18.713789107763613
46	19.731011915013543	19.247209131190406	39.63894847265497	21.382830481141085
47	18.264376260218516	19.38821764071463	41.00067211654188	21.34673398252497
48	20.0084503074973	20.193105175030908	41.77738134359889	18.0210631738729
49	17.32520886990389	16.742571769583517	36.423456247251714	29.508763113260883
50	20.861362285285193	18.050123588123025	40.64641281561904	20.44210131097275
51	18.719628805216793	21.288835940684077	40.77656205912782	19.214973194971314
52	19.206344240755804	19.099981230056937	41.531627354063694	20.16204717512357
53	18.82531534633322	18.340167141381578	43.694638329838185	19.139879182447025
54	17.47588021551184	19.970868312241574	39.2619972434532	23.291254228793385
55	19.06089462473374	20.77903771457211	40.035709810800654	20.1243578498935
56	21.44511927869263	19.569845344687245	37.51330536597583	21.471730010644293
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	2.0
12	12.5
13	64.0
14	105.0
15	163.0
16	221.0
17	275.5
18	330.0
19	380.0
20	430.0
21	561.5
22	735.5
23	778.0
24	868.0
25	958.0
26	1161.5
27	1365.0
28	1582.0
29	2070.5
30	2342.0
31	2459.0
32	2576.0
33	2537.5
34	2499.0
35	2283.5
36	2068.0
37	1731.5
38	1244.0
39	1093.0
40	1012.5
41	932.0
42	910.5
43	889.0
44	932.0
45	975.0
46	1063.0
47	1456.5
48	1762.0
49	1791.5
50	1821.0
51	1711.0
52	1601.0
53	1801.5
54	2033.5
55	2065.0
56	2287.5
57	2510.0
58	2676.0
59	2842.0
60	3214.5
61	3587.0
62	4073.0
63	4606.5
64	4654.0
65	4210.0
66	3766.0
67	3411.0
68	3056.0
69	2668.5
70	2281.0
71	1930.0
72	1376.5
73	1174.0
74	1020.0
75	866.0
76	724.5
77	583.0
78	480.5
79	270.0
80	162.0
81	117.5
82	73.0
83	49.0
84	25.0
85	18.0
86	11.0
87	9.5
88	6.0
89	4.0
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.02500234396974716
14	0.0015626464981091976
15	0.23439697471637966
16	0.04844204144138513
17	0.0
18	0.003125292996218395
19	0.11876113385629902
20	0.08125761790167828
21	0.0
22	0.0
23	0.0
24	0.0
25	0.003125292996218395
26	0.015626464981091975
27	0.12032378035440822
28	0.14532612432415537
29	0.029690283464074756
30	0.02187705097352877
31	0.039066162452729945
32	0.08750820389411508
33	0.18595493327499452
34	0.045316748445166734
35	0.04844204144138513
36	0.03437822295840235
37	0.2093946307466325
38	0.29221489514641996
39	0.17657905428633935
40	0.6609994687001906
41	0.8282026439978748
42	0.11251054786386223
43	0.23439697471637966
44	0.45473013094977655
45	0.20626933775041412
46	0.19533081226364973
47	0.026564990467856365
48	0.14220083132793698
49	0.49692158639872486
50	0.11251054786386223
51	0.31252929962183956
52	0.09688408288277026
53	0.15001406381848298
54	0.22814638872394288
55	0.22814638872394288
56	0.17189111479201177
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	63994.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.12873081851423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.03572802256507	62.91683595337063
2	7.906320782939442	11.563584086008063
3	2.6646437881960767	5.845860549426509
4	1.2543271079960683	3.6690939775603963
5	0.6496004102739433	2.3752226771259806
6	0.3696739176887901	1.6220270650373472
7	0.2692422753109107	1.3782542113323124
8	0.1752211632975768	1.0250961027596337
9	0.12821060729090988	0.8438291089789668
>10	0.5128424291636395	5.861487014407601
>50	0.02136843454848498	1.2782448354533238
>100	0.012821060729090986	1.6204644185392378
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	417	0.6516235897115354	No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	167	0.26096196518423603	No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	122	0.19064287276932212	No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	116	0.18126699378066694	No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	114	0.17814170078444855	No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	101	0.15782729630902897	No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	99	0.1547020033128106	No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG	98	0.1531393568147014	No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	91	0.14220083132793698	No Hit
AGCGAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG	91	0.14220083132793698	No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89	0.1390755383317186	No Hit
AGCGAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	81	0.12657436634684502	No Hit
AGCGAGGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG	76	0.11876113385629902	No Hit
AGCGAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	69	0.10782260836953465	No Hit
AGCGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	67	0.10469731537331625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.006250585992436791	0.0	0.0	0.0	0.0
6	0.006250585992436791	0.0	0.0	0.0	0.0
7	0.006250585992436791	0.0	0.0	0.0	0.0
8	0.006250585992436791	0.0	0.0	0.0	0.0
9	0.006250585992436791	0.0	0.0	0.0	0.0
10	0.007813232490545989	0.0	0.0	0.0	0.0
11	0.01406381848298278	0.0	0.0	0.0	0.0
12	0.015626464981091978	0.0	0.0	0.0	0.0
13	0.017189111479201175	0.0	0.0	0.0	0.0
14	0.021877050973528767	0.0	0.0	0.0	0.0
15	0.02656499046785636	0.0	0.0	0.0	0.0
16	0.02812763696596556	0.0	0.0	0.0	0.0
17	0.02812763696596556	0.0	0.0	0.0	0.0
18	0.031252929962183956	0.0	0.0	0.0	0.0
19	0.03437822295840235	0.0	0.0	0.0	0.0
20	0.039066162452729945	0.0	0.0	0.0	0.0
21	0.04062880895083914	0.0	0.0	0.0	0.0
22	0.04062880895083914	0.0	0.0	0.0	0.0
23	0.04219145544894834	0.0	0.0	0.0	0.0
24	0.04844204144138513	0.0	0.0	0.0	0.0
25	0.05469262743382192	0.0	0.0	0.0	0.0
26	0.06094321342625871	0.0	0.0	0.0	0.0
27	0.06250585992436791	0.0	0.0	0.0	0.0
28	0.0656311529205863	0.0	0.0	0.0	0.0
29	0.0687564459168047	0.0	0.0	0.0	0.0
30	0.0703190924149139	0.0	0.0	0.0	0.0
31	0.0734443854111323	0.0	0.0	0.0	0.0
32	0.07500703190924149	0.0	0.0	0.0	0.0
33	0.07969497140356908	0.0	0.0	0.0	0.0
34	0.08125761790167828	0.0	0.0	0.0	0.0
35	0.08282026439978749	0.0	0.0	0.0	0.0
36	0.08282026439978749	0.0	0.0	0.0	0.0
37	0.08750820389411507	0.0	0.0	0.0	0.0
38	0.08907085039222427	0.0	0.0	0.0	0.0
39	0.09063349689033347	0.0	0.0	0.0	0.0
40	0.09219614338844266	0.0	0.0	0.0	0.0
41	0.09219614338844266	0.0	0.0	0.0	0.0
42	0.09532143638466106	0.0	0.0	0.0	0.0
43	0.09532143638466106	0.0	0.0	0.0	0.0
44	0.09688408288277026	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCTC	15	0.008233233	50.246056	40
CGATTAA	20	4.0544177E-4	50.246056	49
ACGATTA	20	4.0544177E-4	50.246056	48
CAACGAT	20	4.0702432E-4	50.206463	46
AACGATT	20	4.0702432E-4	50.206463	47
GCTACTT	15	0.0082589565	50.206463	46
TCAACGA	20	4.0702432E-4	50.206463	45
TAGAAGG	15	0.00828474	50.166927	15
ATGGTTC	20	4.0861178E-4	50.166927	14
TGGGATC	15	0.00828474	50.166927	15
AAATGAG	15	0.00828474	50.166927	15
ACTACCT	15	0.0083105825	50.12746	13
CCGACTC	15	0.0083105825	50.12746	43
GCAGCTA	15	0.0083105825	50.12746	43
AGACTAC	15	0.008362449	50.048702	11
ATATGCC	25	2.0532589E-5	50.048702	34
AGAGTTA	15	0.008362449	50.048702	10
AAACATG	15	0.008362449	50.048702	10
GATTGTA	15	0.008362449	50.048702	10
GAGTTAT	15	0.008362449	50.048702	11
>>END_MODULE