FastQCFastQC Report
Fri 17 Jun 2016
SRR1526227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27549
Sequences flagged as poor quality0
Sequence length56
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG600.21779374931939455No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATGAGATGGGCAAAA530.19238447856546517No Hit
TTGGACGGGCAGTGTTCGCAGAGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.18512468692148534No Hit
TTGGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.15245562452357617No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATGAGATGGCCAAAA410.14882572870158628No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATGAGATGGGGAAAA410.14882572870158628No Hit
TTGGACGGGAAGCTGAAAGGTTACACATCCTGGGCCATTGGCCTCTCTGTGGCAGA330.119786562125667No Hit
TTGGACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT320.11615666630367709No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC300.10889687465969727No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC290.10526697883770735No Hit
TTGGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA280.10163708301571744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCCC251.9053687E-550.5526838
AACCAAC150.00798809950.5526841
CCCCGAC150.00798809950.5526841
TCACAAT150.00798809950.5526841
GCCTGCC150.00798809950.5526839
GAGCCCC150.00804678550.45940837
CCCAGTT309.854011E-750.18165214
CCAGTTC309.854011E-750.18165215
AATAGCC150.008224769550.18165214
GTGCTTG150.00828474350.08974533
ACTCCTC252.0124384E-550.0897433
AGTGGCA150.00834504149.99817313
CATTCTA301.0107506E-649.99817345
TGTCACG150.00834504149.99817313
GATCACG204.1128477E-449.99817316
GAATAGC150.00834504149.99817313
ACAATTA204.1128477E-449.99817343
ATTATCT252.0344392E-549.9981746
ATTAATA252.0344392E-549.9981719
CTAATCG252.0344392E-549.9981749
Produced by FastQC (version 0.11.5)