##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526226_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 63761 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.19990276187638 39.0 38.0 40.0 35.0 40.0 2 37.8088957199542 39.0 38.0 40.0 35.0 40.0 3 36.26666771223789 39.0 38.0 40.0 27.0 40.0 4 37.80241840623579 39.0 38.0 40.0 35.0 40.0 5 37.93248223835887 39.0 38.0 40.0 35.0 40.0 6 38.091027430561 39.0 38.0 40.0 35.0 40.0 7 37.987092423268145 39.0 38.0 40.0 35.0 40.0 8 37.87382569282163 39.0 38.0 40.0 35.0 40.0 9 37.892253885604056 39.0 38.0 40.0 35.0 40.0 10 37.450369348034066 39.0 38.0 40.0 33.0 40.0 11 36.76502877934788 38.0 36.0 40.0 32.0 40.0 12 34.97623939398692 38.0 35.0 40.0 24.0 40.0 13 34.630103041043895 38.0 33.0 40.0 23.0 40.0 14 34.691441476764794 38.0 33.0 40.0 23.0 40.0 15 34.61595646241433 38.0 33.0 40.0 23.0 40.0 16 33.97162842489924 38.0 33.0 39.0 22.0 40.0 17 33.944197863898 38.0 32.0 39.0 22.0 40.0 18 33.38942300152131 38.0 31.0 39.0 19.0 40.0 19 34.310189614341056 38.0 33.0 39.0 24.0 40.0 20 34.43383886701903 38.0 33.0 39.0 25.0 40.0 21 35.15554963065197 38.0 35.0 39.0 26.0 40.0 22 34.818995937955805 38.0 33.0 39.0 25.0 40.0 23 35.35971832311287 38.0 35.0 39.0 27.0 40.0 24 34.910399774156616 38.0 33.0 39.0 25.0 40.0 25 35.22052665422437 38.0 34.0 39.0 27.0 40.0 26 35.77878326876931 38.0 35.0 39.0 29.0 40.0 27 35.696350433650665 38.0 35.0 39.0 29.0 40.0 28 35.65226392308778 38.0 35.0 39.0 29.0 40.0 29 35.420319631122474 38.0 35.0 39.0 28.0 40.0 30 35.38274180141466 38.0 35.0 39.0 27.0 40.0 31 35.6791298756293 38.0 35.0 39.0 29.0 40.0 32 35.38903091231317 38.0 35.0 39.0 28.0 40.0 33 35.54967770267091 38.0 35.0 39.0 29.0 40.0 34 35.510672668245476 38.0 35.0 39.0 29.0 40.0 35 35.32977839117956 38.0 35.0 39.0 28.0 40.0 36 34.835479368265865 38.0 35.0 39.0 28.0 40.0 37 34.568795972459654 38.0 34.0 39.0 27.0 40.0 38 34.323912736625836 38.0 33.0 39.0 27.0 40.0 39 33.7580182243064 37.0 33.0 39.0 24.0 40.0 40 30.536566239550822 35.0 27.0 39.0 10.0 40.0 41 27.24753375888082 33.0 16.0 38.0 6.0 39.0 42 31.50897884286633 35.0 30.0 38.0 15.0 40.0 43 30.018224306394192 35.0 27.0 38.0 7.0 39.0 44 29.870108687128496 35.0 27.0 38.0 6.0 40.0 45 31.14606107181506 35.0 30.0 38.0 10.0 40.0 46 30.672448675522656 35.0 28.0 38.0 4.0 40.0 47 30.157039569642887 35.0 27.0 38.0 4.0 40.0 48 30.264362227694043 35.0 28.0 38.0 4.0 40.0 49 27.595160050814762 34.0 17.0 38.0 4.0 40.0 50 29.8289079531375 35.0 27.0 38.0 4.0 40.0 51 30.08522451028058 35.0 28.0 39.0 4.0 40.0 52 29.882153667602452 35.0 28.0 39.0 4.0 40.0 53 29.463951318203918 35.0 27.0 39.0 4.0 40.0 54 28.32632800614796 35.0 20.0 39.0 4.0 40.0 55 28.71989146970719 35.0 24.0 39.0 4.0 40.0 56 27.453474694562505 34.0 17.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.021664779263716127 1 2 0.7229279381759497 1 3 2.5021643076336417 1 4 0.6606796371338248 1 5 0.524025889270952 1 6 0.20970493487966735 1 7 0.28455520173461935 1 8 0.4711414322947505 1 9 0.2502408381573389 1 10 0.7716278967004158 1 11 -2.0419061910559435 1 12 -13.963524292064289 1 13 -8.84831915771208 1 14 -5.79149266028373 1 15 -6.0776932480426 1 16 -23.4217246307289 1 17 -4.251121711313662 1 18 -13.021641490335504 1 19 -5.83026292817555 1 20 -5.407484024902818 1 21 -3.7466317598857337 1 22 -1.7124406922802535 1 23 -2.2292850204696464 1 24 -2.373862505044297 1 25 -1.9221201880315917 1 26 1.6106804401821861 1 27 2.085348161185806 1 28 1.6047642400945819 1 29 2.0168888105098404 1 30 2.462290255741536 1 31 1.1078138276417633 1 32 1.2123017892975056 1 33 0.5881501899035015 1 34 1.0625071408562405 1 35 0.39038814575880565 1 36 1.8017863202084499 1 37 1.4104186407225114 1 38 1.7792341164644725 1 39 2.0248805779069627 1 40 4.60997447327021 1 41 -9.392655302035823 1 42 -8.983492159770421 1 43 -17.850944055212473 1 44 -22.550173195968803 1 45 -10.866654492256306 1 46 -17.092170072860938 1 47 -23.58931630193941 1 48 -14.67699059439667 1 49 -20.310609621039962 1 50 -13.434279700081305 1 51 -9.92641469710529 1 52 -9.835534514859802 1 53 -9.419429241641577 1 54 -9.198701765125797 1 55 -9.529113541339441 1 56 -4.264501479661089 2 1 1.1247907260153198 2 2 1.3518872141940435 2 3 3.5673226786743655 2 4 1.3003478120961134 2 5 1.2834527369330928 2 6 1.236401767458851 2 7 1.327239967948799 2 8 1.4657115680413568 2 9 1.4703011699823776 2 10 1.2933473537139903 2 11 1.2332069311160012 2 12 -3.4798138848244697 2 13 -1.2085001531871953 2 14 2.8430473095503572 2 15 1.9072237956979734 2 16 2.2551984461941785 2 17 3.268486131823593 2 18 -2.509725954890559 2 19 2.3469618078727166 2 20 2.0944767594109095 2 21 2.1310454648503168 2 22 2.269610589771034 2 23 1.9657376039647403 2 24 1.7626382491035173 2 25 1.831424306689378 2 26 1.5633199575275896 2 27 2.1773541943683057 2 28 2.085608885645108 2 29 2.1779747833605185 2 30 1.9254878424685344 2 31 1.697406587822762 2 32 2.1578523172009767 2 33 1.6979541114721286 2 34 2.0359611378396494 2 35 1.2398602422897298 2 36 1.7664922025613876 2 37 1.9626056693801246 2 38 2.060983739390565 2 39 2.26107967292959 2 40 5.327319872968555 2 41 -4.654646252262069 2 42 -6.56267768013241 2 43 -12.627414643447768 2 44 -14.25243563940772 2 45 -5.355191445499141 2 46 -9.84450793108115 2 47 -16.761865321547255 2 48 -8.337473248996972 2 49 -12.408799666288832 2 50 -7.192349081680099 2 51 -1.9018294784024228 2 52 -2.2056702614661354 2 53 -2.217317627765258 2 54 -0.6618993518527958 2 55 -1.6627485337979628 2 56 1.196433663626994 3 1 1.3008647511309803 3 2 1.8033082320393845 3 3 3.3768401935454833 3 4 1.4136326766037186 3 5 1.7520581566290616 3 6 1.4728855744648044 3 7 1.250703285860233 3 8 1.4806487788807488 3 9 1.5949541772527027 3 10 1.559441206985639 3 11 2.3677740163770693 3 12 4.0256719049970755 3 13 2.83102397106925 3 14 3.1383546459680716 3 15 3.901275348903525 3 16 5.216996199003162 3 17 4.915544955353006 3 18 5.809242980998405 3 19 4.40842909141535 3 20 4.0681714058087906 3 21 3.6925788422462205 3 22 3.665116207748561 3 23 3.491976863713589 3 24 3.593437984728105 3 25 2.569957023146742 3 26 1.5107092503233588 3 27 1.318134101836911 3 28 1.4419869424858192 3 29 1.5250997007432048 3 30 1.8444567783574968 3 31 1.479032496613243 3 32 1.5045806714640264 3 33 0.6967644221132403 3 34 0.7032446804701848 3 35 0.4822924960901247 3 36 1.7118986797590097 3 37 1.812176059333865 3 38 2.1860333097805196 3 39 2.9877154957980565 3 40 6.63359878902936 3 41 7.265512372985786 3 42 3.6560929741967385 3 43 -1.43521371813382 3 44 2.042739509303452 3 45 4.128879935862393 3 46 3.70327792483425 3 47 -0.5031739798420318 3 48 3.840583591716843 3 49 5.980228754068076 3 50 4.315648274565774 3 51 4.445956377512978 3 52 5.117793056445297 3 53 4.864638750291583 3 54 6.261828341471727 3 55 4.970166205131314 3 56 6.266757523508026 4 1 1.2866238313504752 4 2 1.7506560240435647 4 3 3.741878082231139 4 4 1.559308140413492 4 5 1.5878303019125752 4 6 1.3295618221784196 4 7 1.5254615821758861 4 8 1.4201575324736453 4 9 1.6915050003517322 4 10 1.6150983797057847 4 11 2.1575571363143595 4 12 4.383524008472385 4 13 4.118586030123339 4 14 3.297356475077031 4 15 3.5713824824284757 4 16 4.96915193456627 4 17 4.9620565832599794 4 18 5.616402636080714 4 19 4.150655378324153 4 20 4.514996595252221 4 21 3.590279390978907 4 22 4.453331520003587 4 23 3.7385146217485996 4 24 4.125839343494761 4 25 3.7230795734472437 4 26 3.218313117582305 4 27 3.222497833219194 4 28 3.201067161978898 4 29 2.6922018695438297 4 30 3.360918759021203 4 31 2.4316063142249504 4 32 3.1806977344376364 4 33 2.5842742209112544 4 34 1.999299031136502 4 35 2.2851406800462257 4 36 3.1506235295107743 4 37 3.022523590036762 4 38 3.2968250526600613 4 39 4.585703475939162 4 40 7.4501652902052165 4 41 9.90609246182822 4 42 5.799155425202748 4 43 7.335102456415434 4 44 6.693118396159996 4 45 5.996792138632188 4 46 5.064657595594635 4 47 4.5463426127892745 4 48 5.723128666187705 4 49 7.287780361070947 4 50 4.709429304092815 4 51 3.4701267459477805 4 52 4.125109492295831 4 53 4.315514109580846 4 54 5.484065080431748 4 55 3.3847206864482757 4 56 3.3657907087706356 5 1 1.181972668559773 5 2 1.8204234659040353 5 3 3.602343198510212 5 4 1.7802383729716311 5 5 1.6459698367962972 5 6 1.457468798922605 5 7 1.4208104193851838 5 8 1.6410877650317843 5 9 1.6798770933749907 5 10 1.6267262866825263 5 11 2.587789694453896 5 12 4.418407729402617 5 13 4.072074402216366 5 14 4.343868102984011 5 15 4.652777831265688 5 16 5.259849608984872 5 17 5.252754257678589 5 18 5.569891008173734 5 19 4.266934448091597 5 20 4.5266245022289695 5 21 3.8228375305137874 5 22 4.174261752561726 5 23 3.9013053194230167 5 24 4.067699808611039 5 25 3.9440098060053828 5 26 3.253196838512544 5 27 3.1178466704284986 5 28 2.6545555340719176 5 29 3.5061553579159224 5 30 3.663244340416547 5 31 2.6176528258528577 5 32 2.9597675018794973 5 33 3.23543701160893 5 34 3.057438566020224 5 35 3.227001145162504 5 36 3.5343444597433304 5 37 4.255081729571643 5 38 4.413104122427498 5 39 5.00430812710195 5 40 8.636211801833124 5 41 11.289813392060775 5 42 7.624736820551583 5 43 8.24207920060148 5 44 8.390792814764644 5 45 6.357257254911254 5 46 7.204192479315569 5 47 7.116110054649738 5 48 6.7928961080481685 5 49 10.485454779675603 5 50 7.7791967459532785 5 51 6.4701267459477805 5 52 6.253016469040016 5 53 5.629467597952939 5 54 7.356158103687562 5 55 6.291697430634322 5 56 7.237883732026447 6 1 1.2253646141071357 6 2 1.7923463246675055 6 3 3.2376239699225735 6 4 1.8026433644634068 6 5 1.6550452763879022 6 6 1.2725567172436456 6 7 1.362103669526988 6 8 1.682210850681244 6 9 1.2621232646739102 6 10 1.4517404670568865 6 11 2.5078123830528725 6 12 4.474562011875676 6 13 4.2379847822504 6 14 4.572172130210326 6 15 4.537065976472725 6 16 4.947881373023442 6 17 5.122862028523741 6 18 5.739771893029101 6 19 4.891438134988931 6 20 4.344548495422387 6 21 3.7105289655676756 6 22 4.31351302879542 6 23 3.7050489382545564 6 24 3.8881762691896 6 25 3.86743578445121 6 26 3.380536600282248 6 27 3.315804696520388 6 28 2.624776747911966 6 29 3.8039432195154745 6 30 3.3725466659979446 6 31 2.680046473045145 6 32 3.195728931261229 6 33 2.811727425675862 6 34 2.5171663028324716 6 35 2.6583681332509883 6 36 3.265200954354789 6 37 3.4204248946312035 6 38 3.4287025342255717 6 39 4.178726731753116 6 40 7.7456842919068585 6 41 3.7518099887709297 6 42 5.5427742567398965 6 43 6.892958383811916 6 44 6.455455321854835 6 45 5.354421180038877 6 46 6.099824924012108 6 47 6.085480446028072 6 48 6.65477926176343 6 49 8.64342415006698 6 50 5.763031119180738 6 51 4.622991181568885 6 52 4.826754415721812 6 53 4.520845930340911 6 54 5.781285727056819 6 55 4.639116602500458 6 56 4.6757736923214 7 1 0.7766150000287197 7 2 1.5573972663161584 7 3 3.363897511139001 7 4 1.5329613638459278 7 5 1.3586575023835792 7 6 1.2861411568855132 7 7 1.1844253737566888 7 8 1.5480645092178307 7 9 1.354590075417967 7 10 1.7993786269827936 7 11 2.183020780706471 7 12 3.893827217185951 7 13 3.8829369279743844 7 14 3.9769266840849795 7 15 4.2020860443949175 7 16 4.9463376867005096 7 17 4.940861411049205 7 18 5.413282235727458 7 19 4.489925322392445 7 20 3.714878844295498 7 21 2.6884542511496434 7 22 3.7784713492646986 7 23 3.4119029055284074 7 24 3.5422361642189273 7 25 3.5264354757139458 7 26 3.095263367803092 7 27 2.7938843507346505 7 28 2.217552295920612 7 29 3.198972549555606 7 30 3.6510276786561704 7 31 2.534785590056565 7 32 2.6035708577817616 7 33 2.744268333363422 7 34 2.4146655309893106 7 35 2.804863965297919 7 36 2.434697712613513 7 37 3.0016227952178056 7 38 3.26738731347843 7 39 4.115435592512611 7 40 7.309747274308837 7 41 -1.1526358278391342 7 42 3.5676276065392187 7 43 6.199688856179932 7 44 4.647489900428468 7 45 4.295143625238012 7 46 5.20448685670738 7 47 5.326295512406581 7 48 6.443911710049939 7 49 8.724930787918165 7 50 6.322617411246185 7 51 5.8079248030569985 7 52 5.29202147345568 7 53 6.4543130498222325 7 54 6.308763343605136 7 55 6.7234018757329395 7 56 7.1662028371191795 8 1 0.8424377848388431 8 2 1.8835048612528666 8 3 3.6665873845567205 8 4 1.7084360473902365 8 5 1.4974233251683913 8 6 1.3231664733412103 8 7 1.536798791478212 8 8 1.6230645092178335 8 9 1.6313305817470862 8 10 1.2764355890081092 8 11 2.3773245781748216 8 12 4.567244938704938 8 13 4.708411611518692 8 14 4.4886355448444775 8 15 4.349870854521505 8 16 4.892698446194174 8 17 4.778044955353003 8 18 5.01233286863885 8 19 4.539608866696248 8 20 4.38418264176385 8 21 3.837663111909137 8 22 4.15711058977103 8 23 3.662061133376504 8 24 4.110432366750572 8 25 3.2387772478658476 8 26 2.505231722233468 8 27 2.984707135544774 8 28 3.1525206503509935 8 29 3.3890041951252243 8 30 3.3225466659979475 8 31 2.938583058410998 8 32 3.1225581995539144 8 33 3.0214835232368387 8 34 2.7330199613690596 8 35 2.57147788934855 8 36 3.0642863202084527 8 37 2.8280468458507144 8 38 3.618336680567033 8 39 4.4037267317531175 8 40 6.917025755321493 8 41 -4.481116840497364 8 42 4.354969378691116 8 43 6.399055944787527 8 44 5.75271141941581 8 45 4.4153967897949755 8 46 5.631227363036498 8 47 5.394017031393922 8 48 5.749291456885381 8 49 7.759582686652347 8 50 5.223092094790488 8 51 4.798905815715223 8 52 5.241388562063275 8 53 4.9812117839994485 8 54 5.203541824617794 8 55 3.9321044073785067 8 56 4.573639545979937 9 1 1.2181134605145232 9 2 1.3351940504420554 9 3 2.943952249421585 9 4 1.2736387500929354 9 5 1.2842476494927126 9 6 1.0454637706385057 9 7 1.036460953640372 9 8 1.1021185632718868 9 9 1.520857608774108 9 10 0.8152869403594636 9 11 2.038135388985637 9 12 3.9969746684346674 9 13 4.123614314221392 9 14 4.197419328628257 9 15 4.214060043710688 9 16 4.398441689437419 9 17 4.537166576974627 9 18 5.026522057828039 9 19 4.180825082912463 9 20 3.75918264176385 9 21 3.9359739227199526 9 22 4.245286265446708 9 23 3.0732097820251525 9 24 4.011445880264091 9 25 3.892155626244225 9 26 2.7170560465577935 9 27 2.857004432842075 9 28 2.7177233530536924 9 29 3.3069096005306307 9 30 3.034708828160106 9 31 2.5547992746272143 9 32 2.6766122536079706 9 33 2.5971591989125145 9 34 2.4370740154231143 9 35 2.958302213672873 9 36 2.80178632020845 9 37 3.0209522512561193 9 38 3.6136069508373083 9 39 4.543591596617979 9 40 6.810944674240414 9 41 5.9337480243675 9 42 5.122536946258684 9 43 6.649055944787527 9 44 6.767914122118512 9 45 4.334315708713895 9 46 3.735619254928391 9 47 4.865638653015544 9 48 4.712129294723219 9 49 7.172758362328022 9 50 4.806538040736434 9 51 4.843162572471982 9 52 4.84949667017138 9 53 4.011617189404852 9 54 5.026176959752931 9 55 4.74392873170283 9 56 4.734112518952909 10 1 0.43987368227474377 10 2 1.2998510150990228 10 3 2.7842155896849263 10 4 0.8914488679030512 10 5 0.99838486362993 10 6 0.517397242571974 10 7 0.8871193042987215 10 8 1.1249875861409109 10 9 0.6348562227727257 10 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114 35 1.6173112226818844 114 36 2.5851196535417813 114 37 2.78221351251738 114 38 2.810003347233703 114 39 3.245393398419786 114 40 7.22535908865483 114 41 6.085549826169302 114 42 2.4549693786911178 114 43 -0.7176107218791401 114 44 -3.6181219139175234 114 45 2.5320634564616427 114 46 -1.389605970296838 114 47 -1.3893163019394095 114 48 -1.5090418764479523 114 49 -17.202917313347655 114 50 -1.4560745718761758 114 51 -2.363594184284775 114 52 -1.425278104603393 114 53 -2.243788216000553 114 54 -9.54229150871554 114 55 -2.4137289259548247 114 56 0.7278062126466018 115 1 0.28437326870981394 115 2 0.8234242160915812 115 3 2.6758615781051063 115 4 0.2806134667450735 115 5 0.6857297767812938 115 6 0.5062309894702395 115 7 0.5194600818007942 115 8 0.628709670508151 115 9 0.7035080011019232 115 10 1.0929678470726287 115 11 -0.22428832505098484 115 12 -9.05533570645635 115 13 -4.708120646545826 115 14 -7.2476547777361695 115 15 -7.846096887413982 115 16 -7.602866069934855 115 17 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-5.870718173266653 115 56 -2.851763679826515 116 1 -0.04327650087544299 116 2 0.5888620041100125 116 3 2.5362302416995774 116 4 0.38522176167595035 116 5 0.28849475373981903 116 6 0.3338807590554964 116 7 0.1028702200496383 116 8 0.1480645092178321 116 9 0.2081162960327987 116 10 0.3353641604366828 116 11 -1.386961136110891 116 12 -13.161326489866486 116 13 -8.607659817052738 116 14 -2.929221598012667 116 15 -6.403700574049925 116 16 -3.3162301252343944 116 17 -1.6558836160755668 116 18 -8.762667131361148 116 19 -2.449676847589469 116 20 -3.4836745010932937 116 21 2.082305969051994 116 22 2.8410391611996033 116 23 2.338846847662218 116 24 1.9122180810362863 116 25 2.013777247865846 116 26 1.8213031508049013 116 27 2.2597071355447795 116 28 2.08644922177956 116 29 2.4729327665537966 116 30 1.7296895231407987 116 31 0.9814402012681427 116 32 2.1797010566967714 116 33 0.735769237522554 116 34 1.5401628185119165 116 35 1.4625493179199793 116 36 2.2875006059227374 116 37 2.8155468458507187 116 38 2.5719081091384624 116 39 3.321583874610262 116 40 7.263454326750068 116 41 5.540311730931208 116 42 0.5835408072625476 116 43 -10.593801198069617 116 44 -8.649074294869905 116 45 0.27253964693783317 116 46 -2.6562726369635037 116 47 -1.6988401114632197 116 48 -2.911422828828904 116 49 -10.231488741919083 116 50 -2.5679793337809365 116 51 -0.613594184284775 116 52 -0.07289715222243842 116 53 -0.8366453588576945 116 54 -8.408958175382207 116 55 -0.33753844976434877 116 56 0.5611395459799375 117 1 -0.5047320264819106 117 2 -0.18418381799241246 117 3 1.8809741770095556 117 4 -0.41019602808146516 117 5 -0.6721521465297258 117 6 -1.0537203191116262 117 7 -0.3238144160689558 117 8 -0.3387279436123549 117 9 -0.7514524371208395 117 10 0.058275211649615244 117 11 -3.2915433463534782 117 12 -8.6478494009177 117 13 -13.49418272810395 117 14 -11.086364455155525 117 15 -8.71097820208227 117 16 -14.00517891229639 117 17 -13.329738063514917 117 18 -11.889082225700768 117 19 -6.403315661605642 117 20 -6.571006037481432 117 21 -0.5069123597889771 117 22 -0.6285026177761353 117 23 -0.8584577345480184 117 24 -0.0020676332494247163 117 25 -0.505090676662455 117 26 0.7040524769504515 117 27 0.5880090223372321 117 28 0.373511216388728 117 29 1.2694287234271115 117 30 1.1649994961866241 117 31 0.1970736244487341 117 32 0.7263317844595747 117 33 0.48752125908589505 117 34 0.44905769721811595 117 35 0.6622797761410055 117 36 1.726314622095245 117 37 1.9079996760393954 117 38 2.0791857371708105 117 39 3.178726731753116 117 40 6.193912547774328 117 41 4.191524668936598 117 42 -1.7280494892334097 117 43 -9.096227074080396 117 44 -11.42535461832004 117 45 -0.8581881158654028 117 46 -3.014763203001241 117 47 -3.2371150440777754 117 48 -3.163444392171222 117 49 -8.597256935989165 117 50 -2.865822999549131 117 51 -4.630575316360247 117 52 -5.230309551144273 117 53 -5.223976895245833 117 54 -9.505184590476546 117 55 -4.806810686961114 117 56 -3.521879321944592 118 1 -0.06470507230400813 118 2 0.1995762898243001 118 3 2.2398016702710066 118 4 -0.12549252403833577 118 5 0.40635189659695925 118 6 0.016023616198353352 118 7 -7.012085217894537E-4 118 8 0.2802073663606848 118 9 -0.3811694182529166 118 10 -0.018207268134744936 118 11 -2.3441039932537464 118 12 -8.197040775580774 118 13 -11.882659817052737 118 14 -9.99707874086981 118 15 -6.957272002621355 118 16 -10.791230125234396 118 17 -10.01302647321842 118 18 -11.98409570278972 118 19 -5.196105419018039 118 20 -4.812245929664723 118 21 0.17873454048056914 118 22 0.17675344691388517 118 23 0.8888468476622222 118 24 0.9979323667505753 118 25 0.7637772478658462 118 26 1.1248745793763248 118 27 1.0418499926876308 118 28 1.3007349360652753 118 29 1.3479327665537966 118 30 0.693975237426514 118 31 0.6600116298395662 118 32 -5.1988703718746585 118 33 0.13576923752255254 118 34 0.40087710422620404 118 35 1.0446921750628348 118 36 0.9803577487798805 118 37 0.9191182744221464 118 38 1.5933366805670346 118 39 1.946583874610262 118 40 4.98131146960721 118 41 2.5688831595026365 118 42 -1.8914591927374538 118 43 -9.886658340926758 118 44 -7.55978858058419 118 45 -2.04888892449074 118 46 -8.745558351249219 118 47 -8.32741154003465 118 48 -10.589994257400335 118 49 -11.235060170490513 118 50 -7.389407905209509 118 51 -6.092165612856203 118 52 -5.740754295079583 118 53 -6.5723596445719785 118 54 -11.430386746810777 118 55 -6.098252735478635 118 56 -3.7567175968772055 119 1 -0.07422888182782117 119 2 0.6793381945862009 119 3 2.329087384556722 119 4 0.03760271405690219 119 5 0.3432566585017227 119 6 -0.0018335266587925503 119 7 0.2576321248115434 119 8 0.464731175884495 119 9 0.3271639150804191 119 10 0.6139355890081077 119 11 -4.872675421825178 119 12 -12.132755061295057 119 13 -11.245755055147978 119 14 -11.703031121822193 119 15 -8.708462478811832 119 16 -12.41146822047249 119 17 -14.551121711313659 119 18 -15.379333798027812 119 19 -5.635391133303756 119 20 -7.24081735823615 119 21 1.2751631119091371 119 22 1.4862772564376954 119 23 1.6245611333765098 119 24 1.9145990334172396 119 25 1.347110581199182 119 26 2.2093983889001407 119 27 2.409707135544778 119 28 2.1983539836843207 119 29 2.7015041951252243 119 30 2.972546665997946 119 31 1.6719163917443325 119 32 -4.577441800446085 119 33 1.12148352323684 119 34 1.333019961369061 119 35 1.6506445560152159 119 36 2.8351196535417813 119 37 2.59888017918405 119 38 3.060003347233703 119 39 3.0953933984197803 119 40 7.3253590886548245 119 41 3.368883159502637 119 42 -1.9783639546422158 119 43 -10.367610721879139 119 44 -7.601455247250858 119 45 -4.251269876871692 119 46 -13.906272636963504 119 47 -14.53931630193941 119 48 -12.62570854311462 119 49 -12.252917313347655 119 50 -10.23940790520951 119 51 -7.930260850951441 119 52 -7.4586114379367245 119 53 -8.893788216000551 119 54 -10.058958175382205 119 55 -7.630395592621493 119 56 -3.755527120686729 120 1 -1.5617288818278183 120 2 -0.7498284720804662 120 3 1.079087384556722 120 4 -1.1790639526097664 120 5 -1.6859100081649459 120 6 -1.7726668599921283 120 7 -1.9798678751884538 120 8 -1.9936021574488336 120 9 -1.6936694182529166 120 10 -1.2443977443252265 120 11 -3.647675421825177 120 12 -11.899421727961727 120 13 -8.995755055147978 120 14 -11.211364455155525 120 15 -12.829295812145165 120 16 -16.615634887139155 120 17 -15.646955044646994 120 18 -17.037667131361147 120 19 -7.722891133303754 120 20 -13.86581735823615 120 21 -6.020670221424194 120 22 -3.513722743562301 120 23 -3.804605533290161 120 24 -10.064567633249425 120 25 -3.319556085467486 120 26 0.20523172223347075 120 27 -1.523626197788559 120 28 -0.02664601631567365 120 29 0.44317086179189147 120 30 0.14337999933128032 120 31 -1.744750274922339 120 32 -12.169108467112753 120 33 -1.3285164767631628 120 34 -1.2419800386309419 120 35 -1.8035221106514499 120 36 -1.4273803464582144 120 37 -0.9677864874826199 120 38 -1.9691633194329654 120 39 0.05372673175311604 120 40 2.0586924219881624 120 41 0.7980498261693043 120 42 -2.632530621308881 120 43 -11.33011072187914 120 44 -9.143121913917522 120 45 -7.188769876871692 120 46 -15.052105970296838 120 47 -14.047649635272743 120 48 -11.854875209781287 120 49 -17.273750646680988 120 50 -11.410241238542842 120 51 -9.830260850951444 120 52 -10.112778104603393 120 53 -10.43545488266722 120 54 -12.983958175382206 120 55 -9.830395592621493 120 56 -6.197193787353395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 187.0 5 13.0 6 52.0 7 66.0 8 132.0 9 94.0 10 78.0 11 83.0 12 60.0 13 67.0 14 93.0 15 133.0 16 186.0 17 220.0 18 250.0 19 298.0 20 402.0 21 496.0 22 662.0 23 788.0 24 919.0 25 1134.0 26 1214.0 27 1358.0 28 1588.0 29 1875.0 30 2293.0 31 2670.0 32 3151.0 33 3649.0 34 4162.0 35 4930.0 36 6323.0 37 8424.0 38 9418.0 39 6279.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.58438543937517 0.3136713665093082 0.06273427330186164 0.03920892081366353 8 99.57183858471478 0.29171437085365665 0.09410140995279247 0.042345634478756604 9 95.76857326578944 2.6599331879989334 0.710465645143583 0.861027901068051 10 41.56459277614843 39.10227254905036 10.329198099151519 9.003936575649691 11 31.172660403695048 32.485375072536506 20.369818541114473 15.972145982653974 12 37.667226047270276 15.310299399319332 20.873261084361914 26.149213469048476 13 25.38233494894358 16.126299938826406 25.2286166925478 33.26274841968221 14 23.518248404146867 21.58283536441914 28.839850060383633 26.05906617105036 15 28.22189046908148 18.802545768837902 27.198868547183153 25.77669521489746 16 29.938005179314132 20.199325119673546 25.593659263909597 24.26901043710272 17 30.35711485077085 22.30360251564436 23.818635215884317 23.520647417700474 18 25.910823230501407 22.689418296450807 25.25525007449695 26.144508398550837 19 26.41417629331994 26.346685082872927 23.089841788046208 24.149296835760925 20 25.24416669283673 27.03105863141036 22.931256477090724 24.793518198662188 21 25.846520600366997 22.99681623562993 25.56107965684353 25.595583507159546 22 25.504618810871847 24.163673719044557 24.11662301406816 26.21508445601543 23 25.46540989005819 24.24836498800207 24.78317466790044 25.503050454039304 24 25.25878293601004 24.835319949811794 26.182559598494354 23.723337515683816 25 27.701187283363915 22.96146426386863 25.68108031807274 23.65626813469471 26 24.49574955299727 23.625270554283382 28.408983970638978 23.469995922080365 27 23.770054315406107 19.848670371416908 30.65994788232709 25.721327430849893 28 22.693231595308745 23.93983640274441 31.188670654546023 22.178261347400813 29 24.263390755906993 22.53130000941354 31.767548401267693 21.43776083341178 30 21.750247054948158 20.851437624507852 36.77903091716208 20.619284403381908 31 21.705231782884933 21.78053180641619 38.13475566711115 18.37948074358773 32 24.044453513742603 19.644623039854334 37.876528481956456 18.434394964446607 33 21.909997643917382 19.737689468310688 40.223042488023246 18.129270399748684 34 19.563136504150517 21.661148335870195 43.093194407394044 15.682520752585246 35 18.912388602987324 21.611020459395007 45.021651813731644 14.45493912388603 36 18.35197765818912 23.110595101746238 42.804022781116146 15.733404458948492 37 18.52253441448237 22.348984851342006 43.58382047897417 15.544660255201459 38 21.134490784424734 22.002579102975403 41.63835943888784 15.224570673712021 39 19.07261042409767 21.41543973224808 41.6209676152166 17.890982228437643 40 15.216807519153305 16.19145407155833 38.423505252349734 30.16823315693863 41 12.889549783275855 15.476002151422152 36.24355364318031 35.39089442212168 42 17.418959668300037 21.45212966453072 42.852431209951 18.276479457218244 43 14.88953534082868 17.804858872552874 43.60248447204969 23.703121314568758 44 15.350178912025726 17.84233673292454 41.44768990683964 25.359794448210092 45 17.289073305670815 20.776750911605685 44.184584433547094 17.74959134917641 46 16.6391652130184 21.314412333223327 43.22757060016029 18.818851853597977 47 15.67584529693261 22.09617949321409 43.58358829528516 18.644386914568134 48 16.71772635151058 23.536158112205623 43.21377511743727 16.532340418846523 49 14.257335204250557 19.088085515632145 38.349598751320414 28.304980528796886 50 17.739174140449528 22.36637504515683 43.39139585656619 16.503054957827445 51 16.628913009281106 23.913795815636306 42.52320276860154 16.93408840648104 52 15.887997236050694 23.07897671058624 43.7332160748779 17.299809978485168 53 16.760952919730972 22.722986988497077 44.04110880633604 16.474951285435917 54 15.328008050820808 22.465249386753882 40.99471664884584 21.21202591357947 55 15.984341099896234 24.189541867119456 42.569883344338585 17.256233688645725 56 17.081028413376917 23.596618053809404 40.96680915262761 18.35554438018607 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.0 7 4.0 8 11.5 9 19.0 10 63.0 11 107.0 12 175.0 13 291.0 14 339.0 15 425.5 16 512.0 17 608.0 18 704.0 19 758.0 20 812.0 21 1039.5 22 1552.0 23 1837.0 24 2219.5 25 2602.0 26 2891.5 27 3181.0 28 3258.0 29 3165.0 30 2995.0 31 2778.0 32 2561.0 33 2200.5 34 1840.0 35 1561.5 36 1283.0 37 1183.0 38 1103.5 39 1124.0 40 1125.5 41 1127.0 42 1200.5 43 1274.0 44 1519.5 45 1765.0 46 1761.0 47 1692.0 48 1627.0 49 1821.0 50 2015.0 51 2192.5 52 2370.0 53 2512.0 54 2761.5 55 2869.0 56 2972.5 57 3076.0 58 3369.5 59 3663.0 60 3578.0 61 3493.0 62 3117.0 63 2510.5 64 2280.0 65 1949.5 66 1619.0 67 1403.0 68 1187.0 69 1030.0 70 873.0 71 739.0 72 524.0 73 443.0 74 344.0 75 245.0 76 186.5 77 128.0 78 96.5 79 41.5 80 18.0 81 14.5 82 11.0 83 9.0 84 7.0 85 3.5 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.012546854660372328 14 0.003136713665093082 15 0.19761296090086417 16 0.07214441429714089 17 0.0 18 0.0 19 0.0768494847947805 20 0.11762676244099057 21 0.0 22 0.0 23 0.0 24 0.001568356832546541 25 0.003136713665093082 26 0.004705070497639623 27 0.09253305312024593 28 0.10664826461316479 29 0.03607220714857044 30 0.01568356832546541 31 0.025093709320744655 32 0.08469126895751321 33 0.15056225592446792 34 0.053324132306582395 35 0.03920892081366353 36 0.03764056398111698 37 0.1960446040683176 38 0.2713257320305516 39 0.18820281990558493 40 0.7151707156412227 41 0.857891187402958 42 0.13958375809664214 43 0.2603472342027258 44 0.5050109000799862 45 0.21486488605887613 46 0.20074967456595724 47 0.040777277646210065 48 0.17251925158011952 49 0.5238311820705447 50 0.14742554225937485 51 0.2995561550163893 52 0.13174197393390946 53 0.1960446040683176 54 0.25877887737017924 55 0.24309530904471385 56 0.2023180313985038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 63761.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.8984488950926 #Duplication Level Percentage of deduplicated Percentage of total 1 86.29700979981573 64.63512178290804 2 7.929893625931821 11.8787346497075 3 2.646787838177402 5.947209109016483 4 1.176815478683307 3.5256661595646244 5 0.5779378507412681 2.1643324289142263 6 0.36854007873356226 1.6561848151691472 7 0.21986766060809113 1.1527422719217075 8 0.1717061730463188 1.0288420821505309 9 0.1214507077644694 0.8186822665892944 >10 0.46695703157718405 5.461018490927056 >50 0.018845799480693527 1.1025548532802183 >100 0.004187955440154116 0.628911089851163 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 291 0.45639183827104346 No Hit TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 110 0.17251925158011952 No Hit TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 99 0.15526732642210755 No Hit TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 94 0.14742554225937485 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 83 0.1301736171013629 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 82 0.12860526026881636 No Hit TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 78 0.1223318329386302 No Hit TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 75 0.11762676244099057 No Hit TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 69 0.10821662144571134 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 67 0.10507990778061825 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.01568356832546541 0.0 0.0 0.0 0.0 11 0.020388638823105033 0.0 0.0 0.0 0.0 12 0.023525352488198113 0.0 0.0 0.0 0.0 13 0.02823042298583774 0.0 0.0 0.0 0.0 14 0.03136713665093082 0.0 0.0 0.0 0.0 15 0.03607220714857044 0.0 0.0 0.0 0.0 16 0.042345634478756604 0.0 0.0 0.0 0.0 17 0.04861906180894277 0.0 0.0 0.0 0.0 18 0.053324132306582395 0.0 0.0 0.0 0.0 19 0.06273427330186164 0.0 0.0 0.0 0.0 20 0.07371277112968742 0.0 0.0 0.0 0.0 21 0.07841784162732705 0.0 0.0 0.0 0.0 22 0.09096469628769938 0.0 0.0 0.0 0.0 23 0.10194319411552516 0.0 0.0 0.0 0.0 24 0.1223318329386302 0.0 0.0 0.0 0.0 25 0.13801540126409562 0.0 0.0 0.0 0.0 26 0.15213061275701448 0.0 0.0 0.0 0.0 27 0.1678141810824799 0.0 0.0 0.0 0.0 28 0.1756559652452126 0.0 0.0 0.0 0.0 29 0.18036103574285223 0.0 0.0 0.0 0.0 30 0.18506610624049183 0.0 0.0 0.0 0.0 31 0.191339533570678 0.0 0.0 0.0 0.0 32 0.19761296090086417 0.0 0.0 0.0 0.0 33 0.20074967456595724 0.0 0.0 0.0 0.0 34 0.2023180313985038 0.0 0.0 0.0 0.0 35 0.20388638823105032 0.0 0.0 0.0 0.0 36 0.20859145872868995 0.0 0.0 0.0 0.0 37 0.21172817239378303 0.0 0.0 0.0 0.0 38 0.2148648860588761 0.0 0.0 0.0 0.0 39 0.2148648860588761 0.0 0.0 0.0 0.0 40 0.2180015997239692 0.0 0.0 0.0 0.0 41 0.2180015997239692 0.0 0.0 0.0 0.0 42 0.22427502705415536 0.0 0.0 0.0 0.0 43 0.228980097551795 0.0 0.0 0.0 0.0 44 0.2336851680494346 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAAAGT 15 0.008259848 50.205048 40 CGCACCC 15 0.008259848 50.205048 40 TATGCTT 55 0.0 50.086548 35 GTCTCAA 55 0.0 50.086548 42 ATATGCT 70 0.0 50.086544 34 AACGATT 15 0.008363433 50.04717 47 ACCGTGC 15 0.008363433 50.04717 46 ACCAAGC 20 4.1346365E-4 50.04717 48 TAAGAAG 15 0.008363433 50.04717 48 TGATATG 15 0.008363433 50.04717 50 AAGATTA 40 2.59206E-9 50.04717 48 ACGATTA 15 0.008363433 50.04717 48 GATTAAG 55 0.0 50.04717 50 AGATTAA 40 2.59206E-9 50.04717 49 TACCAAG 15 0.008363433 50.04717 47 AAAGATT 45 1.3096724E-10 50.04717 47 TGTAAGA 15 0.008363433 50.04717 46 CAACGAT 15 0.008363433 50.04717 46 CGATTAA 15 0.008363433 50.04717 49 TGGTTGA 80 0.0 49.9686 14 >>END_MODULE