FastQCFastQC Report
Fri 17 Jun 2016
SRR1526225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54913
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG8501.5479030466374084No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2620.4771183508458835No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2300.41884435379600454No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG2290.41702329138819594No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2140.38970735527106515No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1750.31868592136652524No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1730.3150437965509078No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1240.22581173856828074No Hit
ACGAGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC830.1511481798481234No Hit
ACGAGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC760.1384007429934624No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG670.12201118132318393No Hit
ACGAGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC660.12019011891537523No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG560.10197949483728808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA451.1641532E-1050.6014841
TCGTCTC309.538635E-750.5546340
TGCTCGT309.72339E-750.4145937
GTCGTCT150.00817811450.3216643
CCAAAAC251.9843206E-550.3216643
TCGTCTT150.00817811450.3216644
CGACTCC251.9843206E-550.3216644
CCGAATC150.00817811450.3216643
ATGCTCG309.911182E-750.27532636
AGTTGTC150.00820798750.27532642
GCGTTCA150.00820798750.27532645
CGTTCAG150.00823794250.22907346
GTTCAGC150.00823794250.22907347
AACGATT550.050.2290747
TATGCCC150.00826797750.18290735
ACGATTA507.2759576E-1250.18290748
ATATGCC507.2759576E-1250.09082834
TAGAGCG355.0924427E-850.0908234
ATCCCAT150.00841937949.95334214
TGACACG150.00841937949.95334214