##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526225_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54913 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.30029319104766 38.0 38.0 39.0 34.0 40.0 2 36.5118460109628 39.0 38.0 39.0 31.0 40.0 3 35.82820097244733 38.0 35.0 39.0 28.0 40.0 4 36.67067907417187 38.0 36.0 39.0 33.0 40.0 5 35.399486460401 38.0 35.0 39.0 28.0 40.0 6 35.93879409247355 39.0 36.0 39.0 29.0 40.0 7 36.28343015315135 38.0 36.0 39.0 31.0 40.0 8 36.393404111958915 39.0 36.0 39.0 31.0 40.0 9 36.47289348605977 39.0 37.0 39.0 32.0 40.0 10 35.35823939686413 39.0 35.0 39.0 28.0 40.0 11 34.85502522171435 38.0 34.0 39.0 28.0 40.0 12 33.17258208438803 37.0 32.0 39.0 18.0 40.0 13 33.09354797588913 37.0 32.0 39.0 18.0 40.0 14 33.225465736710795 37.0 32.0 39.0 19.0 40.0 15 33.20335803908 38.0 32.0 39.0 18.0 40.0 16 32.66346766703695 36.0 30.0 39.0 18.0 40.0 17 32.66153734088467 36.0 30.0 39.0 19.0 40.0 18 32.232422195108626 36.0 30.0 39.0 17.0 40.0 19 32.925900970626266 36.0 30.0 39.0 22.0 40.0 20 32.73030065740353 36.0 30.0 39.0 21.0 40.0 21 33.51581592701182 37.0 32.0 39.0 23.0 40.0 22 33.17817274598001 36.0 32.0 39.0 22.0 40.0 23 33.50281354142007 37.0 32.0 39.0 23.0 40.0 24 33.12270318503815 36.0 32.0 39.0 21.0 40.0 25 33.16799300712035 36.0 32.0 39.0 22.0 40.0 26 33.712654562671865 37.0 33.0 39.0 23.0 40.0 27 33.573470762843044 37.0 33.0 39.0 23.0 40.0 28 33.38562817547757 37.0 33.0 39.0 23.0 40.0 29 33.283630470016206 37.0 33.0 39.0 23.0 40.0 30 32.87848050552692 36.0 32.0 39.0 20.0 40.0 31 33.004807604756614 36.0 32.0 39.0 22.0 40.0 32 32.78030703112196 36.0 32.0 39.0 19.0 39.0 33 32.87676870686359 36.0 32.0 39.0 20.0 39.0 34 32.44856409229144 36.0 31.0 38.0 18.0 39.0 35 32.369602826288855 36.0 31.0 38.0 17.0 39.0 36 31.6492998015042 35.0 30.0 38.0 15.0 39.0 37 31.33500264054049 35.0 30.0 38.0 11.0 39.0 38 31.043723708411488 35.0 30.0 38.0 10.0 39.0 39 30.647897583450185 35.0 29.0 38.0 4.0 39.0 40 27.535865824121792 33.0 18.0 38.0 4.0 39.0 41 24.569755795531112 30.0 11.0 36.0 4.0 39.0 42 27.997541565749458 32.0 24.0 36.0 4.0 39.0 43 26.63567825469379 31.0 20.0 36.0 4.0 39.0 44 26.70229271757143 32.0 19.0 36.0 4.0 39.0 45 27.53504634603828 32.0 24.0 36.0 4.0 39.0 46 27.048003205069836 33.0 21.0 37.0 4.0 39.0 47 26.61442645639466 32.0 18.0 36.0 4.0 39.0 48 26.663504088285105 32.0 19.0 36.0 4.0 39.0 49 24.547247464170596 31.0 8.0 36.0 4.0 39.0 50 26.21799938083878 32.0 17.0 36.0 4.0 39.0 51 26.411614736037006 33.0 10.0 38.0 4.0 39.0 52 26.126964471072423 33.0 4.0 38.0 4.0 39.0 53 25.74696337843498 32.0 4.0 37.0 4.0 39.0 54 24.897055342086574 32.0 4.0 37.0 4.0 39.0 55 25.101633492979804 32.0 4.0 37.0 4.0 39.0 56 24.25573179392858 31.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.1707605133450443 1 2 2.0306357884803816 1 3 2.528281543577819 1 4 1.4210676777884643 1 5 3.217812440133507 1 6 2.244560267943953 1 7 2.2131500991777457 1 8 1.9160284192479153 1 9 1.9417846385018294 1 10 1.686887838862262 1 11 -3.5089442420700685 1 12 -12.659853581016105 1 13 -8.927943604522802 1 14 -5.729344229759413 1 15 -6.3439056921101695 1 16 -21.11801950116857 1 17 -3.42584534746835 1 18 -9.623767425470518 1 19 -5.062045491837782 1 20 -4.6896614439960445 1 21 -5.436125607769803 1 22 -3.0491204781860084 1 23 -2.888981473186149 1 24 -3.696451563789161 1 25 -2.399624955789264 1 26 2.912749417060013 1 27 2.489498827925594 1 28 2.32882592875994 1 29 2.951399050823298 1 30 3.4915877624145075 1 31 2.5272576975722387 1 32 3.742757293198821 1 33 2.86841949259556 1 34 2.1491988454991926 1 35 2.40347836237774 1 36 2.2625729390150795 1 37 2.7720315049169777 1 38 1.0615312892621063 1 39 1.0257941368955947 1 40 2.9050816915084674 1 41 -11.288048552843017 1 42 -11.33064709678609 1 43 -16.47418574802681 1 44 -19.73908687670304 1 45 -13.086793811428844 1 46 -17.471416608863265 1 47 -20.031773173742724 1 48 -15.46392533185101 1 49 -17.05261182041058 1 50 -14.562084705499895 1 51 -12.730309248619859 1 52 -12.226299290946557 1 53 -11.821480890386079 1 54 -10.983155606046031 1 55 -11.60559057731476 1 56 -8.452894465510882 2 1 1.7919857074779202 2 2 2.4900835883078187 2 3 3.1643902579867955 2 4 2.165675097978287 2 5 3.29848543409382 2 6 3.17618235595085 2 7 2.682520245855912 2 8 2.546097444269485 2 9 2.575304914601915 2 10 3.1213140683704594 2 11 1.972936689767721 2 12 -1.5138225197563955 2 13 -1.7924819996910522 2 14 3.291578979904088 2 15 3.7143341698397876 2 16 2.5081668663896686 2 17 3.150082176258998 2 18 -1.6459848542884643 2 19 2.453269434823131 2 20 3.1125387285665056 2 21 3.511242813282827 2 22 3.733666407059893 2 23 3.566367965985549 2 24 1.906654562181501 2 25 3.431479444555862 2 26 3.6981247406148015 2 27 3.0425618132577767 2 28 3.2591537976123988 2 29 2.8800012941364983 2 30 2.7098793931306453 2 31 2.845204203180522 2 32 2.415535550317024 2 33 2.3054341604126485 2 34 2.065290303652773 2 35 2.4228916496943924 2 36 3.0248680209822894 2 37 2.787993541155121 2 38 3.3540248181663337 2 39 3.250341160191539 2 40 4.2860135292996695 2 41 -7.906038199089782 2 42 -8.941949944068227 2 43 -12.97871551506736 2 44 -14.543444081189667 2 45 -8.428899074586738 2 46 -12.530303580390447 2 47 -15.975258937332026 2 48 -10.902449922014945 2 49 -13.184406470971409 2 50 -10.424034662359258 2 51 -8.27948957648871 2 52 -7.775263915623004 2 53 -7.617209967176414 2 54 -6.644501593966652 2 55 -7.3171954090662705 2 56 -5.3282180202994915 3 1 1.7866559273313527 3 2 2.6116691979014277 3 3 3.39173869236388 3 4 1.9085132059063312 3 5 3.4020830356927476 3 6 2.9175214632126796 3 7 2.6638660153429257 3 8 2.7674498759816757 3 9 2.528835893949015 3 10 2.9884026759653963 3 11 3.2524170362034326 3 12 5.187210125147004 3 13 3.1448930835867657 3 14 4.302405095826806 3 15 3.141049692824467 3 16 5.344109571253096 3 17 5.099782376125759 3 18 5.598685365564968 3 19 4.750571233623933 3 20 5.150346856481228 3 21 4.260076923875765 3 22 4.793792989338371 3 23 4.330358638870294 3 24 4.503589938597223 3 25 3.957295567140804 3 26 3.7789042209612376 3 27 3.7380981223850256 3 28 3.2686474684984717 3 29 3.6816335393063824 3 30 3.9537168348361753 3 31 3.441140245818765 3 32 3.737154471036547 3 33 3.446340223037563 3 34 3.366256326304338 3 35 2.0461428155837993 3 36 4.145121185539253 3 37 4.10944590624506 3 38 4.232106097313572 3 39 4.826790194168886 3 40 7.722056167540842 3 41 7.6820697289581865 3 42 4.923139995971745 3 43 0.0547621664782767 3 44 2.0386678441934123 3 45 4.672699859457232 3 46 3.4935138746395324 3 47 -1.1994428147470835 3 48 3.126031090643284 3 49 5.443341696916665 3 50 3.9390566101257534 3 51 6.461016752625213 3 52 6.507047876515571 3 53 6.30800655514204 3 54 7.192607000303834 3 55 5.836202325777165 3 56 6.772048468707837 4 1 1.7613394716351465 4 2 3.1306565396735806 4 3 3.3537640088195744 4 4 2.123703079324052 4 5 3.2628425293636383 4 6 3.0061290581493907 4 7 2.6005748761024137 4 8 3.0206144329437024 4 9 2.528835893949015 4 10 3.1909343215350177 4 11 3.024568934937605 4 12 5.769488606159662 4 13 5.917044982320938 4 14 5.049240538864787 4 15 4.533454756115603 4 16 5.774489318088538 4 17 6.0618076925814535 4 18 5.864508150375094 4 19 5.168292752611276 4 20 4.8971822995192085 4 21 3.9183047719770343 4 22 5.122906913389009 4 23 3.9759282591234637 4 24 4.63017221707824 4 25 3.6408398709382723 4 26 3.1459928285561745 4 27 3.206452552764773 4 28 3.5218120254604983 4 29 3.972772779812715 4 30 3.662577594329843 4 31 2.694304802780792 4 32 3.129559534327683 4 33 2.68684655215149 4 34 2.948534807317003 4 35 2.995509904191394 4 36 5.056513590602542 4 37 5.34995223535898 4 38 4.523245337819898 4 39 5.459701586573949 4 40 7.911929585262357 4 41 10.390930488451854 4 42 7.97377290736415 4 43 8.725648242427646 4 44 7.342465312547844 4 45 7.027130239204066 4 46 7.531488558183838 4 47 5.268911615632664 4 48 8.391853875453407 4 49 8.329417646283755 4 50 6.470702179746006 4 51 6.359750929840402 4 52 5.9247693955029135 4 53 6.599145795648369 4 54 7.268556367392442 4 55 5.127341566283494 4 56 6.164453531998976 5 1 1.6655237191738266 5 2 2.358504640939401 5 3 3.2484545854721745 5 4 1.8520786067502115 5 5 3.2765556095324158 5 6 2.8092232916233684 5 7 2.177403286791588 5 8 2.347267034912761 5 9 2.6571762596311572 5 10 2.3713140683704594 5 11 2.8464747014636274 5 12 4.835592684921963 5 13 4.350061859958075 5 14 4.618333365869006 5 15 4.353954052880724 5 16 5.035213650015397 5 17 4.7282985505280095 5 18 4.880330935185221 5 19 3.9905501365775216 5 20 3.9831527636542248 5 21 3.4666521700079755 5 22 4.011444184837671 5 23 4.101455685283796 5 24 4.264841696684428 5 25 3.8057484504038186 5 26 2.7558732786264954 5 27 3.233351286941989 5 28 2.2035982420568416 5 29 2.7535393058324047 5 30 2.6309320247095904 5 31 1.8992977704319856 5 32 3.30150046259773 5 33 1.960404920646564 5 34 1.8101733445884491 5 35 2.335172351448783 5 36 3.3165346876489536 5 37 4.169572488523539 5 38 2.7407207245990826 5 39 4.41627683270616 5 40 6.893469669650543 5 41 9.108581683951154 5 42 6.797085143651067 5 43 7.902863432301061 5 44 8.479947731675828 5 45 6.913206188571156 5 46 5.846889402065695 5 47 5.704565624071485 5 48 6.694772300207276 5 49 8.526850838970113 5 50 6.817924401968227 5 51 6.803843756844621 5 52 6.367279944026119 5 53 6.982936231653994 5 54 7.121580277378378 5 55 4.597278275144255 5 56 4.798243968004602 6 1 1.5716348302849354 6 2 2.6518379742727376 6 3 3.3112323632499496 6 4 1.8337452734168807 6 5 3.173222276199077 6 6 3.1514455138455872 6 7 2.524625509013809 6 8 2.62837814602387 6 9 2.7382873707422632 6 10 3.2879807350371237 6 11 3.2525858125747362 6 12 4.746703796033074 6 13 4.918395193291403 6 14 4.896666699202335 6 15 4.364509608436279 6 16 5.174658094459847 6 17 4.7455207727502255 6 18 5.063664268518551 6 19 4.562216803244187 6 20 4.828152763654224 6 21 3.7105410588968653 6 22 3.0369997403932274 6 23 4.618122351950461 6 24 4.279286141128871 6 25 3.557970672626041 6 26 3.0386510564042766 6 27 2.531684620275321 6 28 2.4691537976123996 6 29 2.7363170836101816 6 30 2.0375986913762567 6 31 2.284853325987541 6 32 2.391500462597726 6 33 2.3492938095354567 6 34 2.0707289001440046 6 35 1.8485056847821113 6 36 3.511534687648954 6 37 3.799572488523541 6 38 3.490165169043525 6 39 4.790165721595045 6 40 6.995136336317209 6 41 4.394137239506708 6 42 5.745418476984405 6 43 7.702863432301058 6 44 7.0921699538980505 6 45 5.96653952190449 6 46 5.643556068732362 6 47 6.174565624071484 6 48 5.407550077985054 6 49 7.87962861674789 6 50 6.52403551307934 6 51 5.367177090177957 6 52 5.498946610692787 6 53 5.600158453876215 6 54 6.866024721822821 6 55 6.042278275144255 6 56 5.992132856893491 7 1 2.14724886537266 7 2 2.9769256935709834 7 3 3.4933376264078504 7 4 2.691990887451965 7 5 3.7853275393569774 7 6 3.005129724371905 7 7 3.300941298487494 7 8 2.8092553390063273 7 9 2.5884628093387576 7 10 3.660787752580987 7 11 3.6176735318729882 7 12 5.40723011182255 7 13 5.733833789782636 7 14 5.186315822009355 7 15 5.67486048562926 7 16 5.955535287442299 7 17 6.084292702574793 7 18 5.472436198343118 7 19 5.071690487454713 7 20 5.1914860969875605 7 21 4.5770322869670395 7 22 4.52314009127042 7 23 4.447946913353974 7 24 4.051391404286768 7 25 4.076216286661122 7 26 3.2244405300884864 7 27 3.0030881290472493 7 28 3.101259060770296 7 29 2.840527609925971 7 30 2.7098793931306453 7 31 2.476783150548947 7 32 2.520798708211764 7 33 2.515960476202121 7 34 2.7889745141790883 7 35 2.1465758602207075 7 36 3.735394336771762 7 37 3.709046172734066 7 38 4.011919555008443 7 39 4.000341160191539 7 40 7.101803002983878 7 41 0.8044881166996909 7 42 4.189629003300194 7 43 6.2186529059852695 7 44 4.996029603020858 7 45 5.097416714886947 7 46 4.9828543143463975 7 47 5.195793694246923 7 48 4.952813235879791 7 49 7.223488265870696 7 50 5.286491653430215 7 51 4.233668318248132 7 52 5.461578189640157 7 53 5.001211085455164 7 54 5.487077353401769 7 55 4.288067748828464 7 56 3.8691504007531385 8 1 1.9943578349797733 8 2 2.9359694296717933 8 3 3.742781659024601 8 4 2.792806306280731 8 5 4.129841994508936 8 6 3.1863750913103814 8 7 2.806315649858881 8 8 2.9665941084651877 8 9 2.816409436469968 8 10 3.776243645835251 8 11 3.365449662339998 8 12 5.566422105892229 8 13 4.919897540709243 8 14 4.966338060704686 8 15 5.768077683553649 8 16 6.155127578027919 8 17 5.8443001154732315 8 18 6.341598540819021 8 19 4.831512577892074 8 20 4.342894547691785 8 21 3.9864096034978047 8 22 3.9554973929753885 8 23 4.8538031031241715 8 24 4.7057650143683105 8 25 3.8460457899969285 8 26 3.8628764085169536 8 27 2.9358160756743814 8 28 3.311970699020847 8 29 4.374626942765111 8 30 4.553467235977198 8 31 3.730674922231671 8 32 4.31675867856017 8 33 4.593425264934513 8 34 3.544719510472639 8 35 3.051134792763335 8 36 3.5671215421090494 8 37 3.257600657537626 8 38 3.6646252629402447 8 39 3.369132857745285 8 40 6.566591735378246 8 41 -0.4896186290378921 8 42 3.773024110787219 8 43 6.100046530892609 8 44 5.531982160470822 8 45 4.6878540758951 8 46 3.7541663973708594 8 47 4.492124309517493 8 48 4.805296556858295 8 49 6.379159133179815 8 50 5.28760358819671 8 51 5.431778029145093 8 52 4.518101540270251 8 53 5.150580989087484 8 54 5.009686693653805 8 55 3.088475458242847 8 56 3.7158887254380915 9 1 1.6183014969516023 9 2 2.2013617837965427 9 3 2.822660934678524 9 4 1.3032690829406874 9 5 3.5360794190562217 9 6 2.0457312281313023 9 7 2.6531969375852356 9 8 2.532187669833398 9 9 2.60876356121846 9 10 2.5070283540847456 9 11 2.742109622098546 9 12 4.818132367461651 9 13 4.8774428123390265 9 14 5.567142889678529 9 15 4.451176275102945 9 16 5.749896189697939 9 17 4.7636160108454675 9 18 5.7446166494709345 9 19 4.867931088958475 9 20 4.655771811273276 9 21 3.3495886779444817 9 22 4.126523549917039 9 23 3.1847890186171313 9 24 4.016428998271728 9 25 3.3874944821498474 9 26 3.04436534211856 9 27 3.02977985837056 9 28 2.809153797612396 9 29 3.2277456550387527 9 30 1.9880748818524445 9 31 1.6000914212256347 9 32 0.9753099864072539 9 33 0.10167476191641 9 34 0.6431098525249546 9 35 1.5161247324011597 9 36 1.7677251638394296 9 37 1.01957248852354 9 38 2.672069930948293 9 39 2.377784769214095 9 40 4.130374431555307 9 41 1.2093753347448057 9 42 3.8054184769844035 9 43 3.8885777180153447 9 44 4.171217572945672 9 45 2.570349045714014 9 46 4.215937021113312 9 47 3.1307561002619586 9 48 2.904692935127912 9 49 5.241533378652651 9 50 3.127845036888864 9 51 2.5633675663684308 9 52 2.001803753549929 9 53 1.7087298824476456 9 54 1.6660247218228221 9 55 0.5508497037156843 9 56 0.20260904736968044 10 1 1.4922145404298632 10 2 2.8077800032582445 10 3 3.368623667597781 10 4 1.6836003458806488 10 5 2.69322227619908 10 6 2.944488992106457 10 7 2.7565095669848247 10 8 2.6648998851543055 10 9 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3.688803257754305 115 38 3.4511908100691677 115 39 3.4173452087745346 115 40 7.2171876183684915 115 41 6.6384962138656824 115 42 2.447726169292096 115 43 -2.5202134907758627 115 44 -3.291419789691691 115 45 2.586283111648079 115 46 -5.012084956908666 115 47 -4.385177965672106 115 48 -4.921680691245715 115 49 -13.275499588380315 115 50 -3.1639132048693774 115 51 -4.202566499565634 115 52 -2.610284158537983 115 53 -3.227533853816091 115 54 -7.472436816638718 115 55 -3.3354140325480515 115 56 -0.37376457900394655 116 1 1.2832137776533585 116 2 2.6742941146236134 116 3 3.1863200825481997 116 4 1.814797904995828 116 5 3.114274907778025 116 6 3.0840770927929597 116 7 2.6123448072594257 116 8 2.515395689883519 116 9 2.5095154409177027 116 10 3.0248228403002813 116 11 -2.1937299768989433 116 12 -13.316454098703765 116 13 -7.704762701445436 116 14 -3.5461403183415214 116 15 -4.838297409107582 116 16 -3.1804296248384 116 17 -1.9814967711094198 116 18 -8.325809415691971 116 19 -1.8756779335979203 116 20 -3.2602682889773504 116 21 3.7217691290722996 116 22 3.352087459691475 116 23 3.1760170887925696 116 24 3.0996370183218502 116 25 3.22533909367867 116 26 3.7025107055270823 116 27 3.814491637819181 116 28 3.7898555519983645 116 29 3.937018837996149 116 30 3.7800548317271314 116 31 3.4329235014261386 116 32 3.972553094176675 116 33 4.138767493745984 116 34 3.7626587247054104 116 35 4.0456986672382556 116 36 5.402061003438426 116 37 5.235361962207751 116 38 6.38911253746458 116 39 6.272270984752943 116 40 9.575487213510193 116 41 6.387821450033027 116 42 2.4878746173352795 116 43 -4.3427506027866585 116 44 -5.793444081189666 116 45 1.8781184692729092 116 46 -3.2013562119693937 116 47 -1.7735045513671146 116 48 -3.5559586939447705 116 49 -5.636160856936321 116 50 -1.581929399201364 116 51 0.7292823533358508 116 52 1.413332575605068 116 53 0.5187549451042841 116 54 -4.0918700150192855 116 55 0.7222782751442551 116 56 1.5577468919812105 117 1 0.7933014969516066 117 2 1.8335046409394025 117 3 2.3762323632499545 117 4 1.4604119400835458 117 5 2.8432222761990786 117 6 2.456445513845587 117 7 1.8746255090138106 117 8 1.9250448126905368 117 9 1.9016207040755972 117 10 1.4213140683704566 117 11 -3.800747520758595 117 12 -10.253296203966922 117 13 -12.608271473375261 117 14 -12.789999967464329 117 15 -8.098823724897056 117 16 -14.15367523887349 117 17 -13.957812560583104 117 18 -15.594669064814779 117 19 -5.892783196755815 117 20 -6.5585139030124395 117 21 -0.11112560776980018 117 22 1.0086664070598914 117 23 0.4847890186171284 117 24 -0.1157138588711284 117 25 0.9696373392927029 117 26 3.3086510564042726 117 27 3.3833512869419877 117 28 3.934153797612396 117 29 3.5813170836101804 117 30 3.5059320247095904 117 31 3.296519992654204 117 32 3.8181671292643955 117 33 4.265960476202121 117 34 3.8323955668106677 117 35 4.251839018115447 117 36 5.174868020982288 117 37 5.26957248852354 117 38 4.6184985023768625 117 39 5.5634990549283785 117 40 8.601803002983878 117 41 6.130803906173377 117 42 2.005418476984403 117 43 -4.672136567698939 117 44 -6.264496712768615 117 45 4.638206188571157 117 46 -0.6197772646009732 117 47 0.5878989574048177 117 48 0.09755007798505488 117 49 -4.0370380499187775 117 50 0.9207021797460051 117 51 2.1955104235112906 117 52 0.43394661069278584 117 53 0.1801584538762171 117 54 -6.458975278177178 117 55 1.3972782751442558 117 56 1.4454661902268242 118 1 0.7085453993906299 118 2 1.6024070799637897 118 3 2.3707445583719036 118 4 1.2799241352054977 118 5 2.229807642052741 118 6 2.1046162455529043 118 7 1.914869411452834 118 8 1.6189472517149284 118 9 1.8150353382219393 118 10 1.694484800077774 118 11 -4.342210935392739 118 12 -8.760613277137654 118 13 -12.327783668497212 118 14 -11.553414601610672 118 15 -7.731750554165348 118 16 -15.161602068141782 118 17 -10.727324755705055 118 18 -16.211132479448928 118 19 -7.108027099194839 118 20 -5.77802609813439 118 21 -0.6312475589893154 118 22 0.15439811437696704 118 23 0.9530817015439581 118 24 0.7153837021044822 118 25 0.9690275831951425 118 26 2.573285202745737 118 27 2.480302506454187 118 28 2.0457391634660596 118 29 2.314853668976035 118 30 1.5943466588559296 118 31 0.9203004804590833 118 32 -2.9037840902478003 118 33 1.5037653542508984 118 34 0.4622736155911582 118 35 1.1115951156764226 118 36 2.9638924112261904 118 37 3.3098163909625633 118 38 3.850815575547596 118 39 4.422035640294233 118 40 6.492046905422903 118 41 2.3612917110514253 118 42 -4.470191279113159 118 43 -7.2678682750160135 118 44 -9.019374761549102 118 45 -4.525818201672745 118 46 -12.813679703625363 118 47 -13.456003481619573 118 48 -12.152449922014945 118 49 -12.767525854796826 118 50 -11.13905391781497 118 51 -10.023392015513101 118 52 -10.584955828331603 118 53 -9.871061058318906 118 54 -13.64129235134791 118 55 -8.838697334611842 118 56 -7.622826492700005 119 1 1.1183014969516023 119 2 2.158504640939398 119 3 2.8845656965832873 119 4 1.8604119400835444 119 5 3.248777831754637 119 6 2.7425566249566984 119 7 2.235736620124918 119 8 2.1528225904683183 119 9 2.2182873707422672 119 10 2.4435362905926823 119 11 -5.200747520758593 119 12 -11.853296203966924 119 13 -11.174938140041927 119 14 -11.156666634130996 119 15 -9.448823724897053 119 16 -14.3897863499846 119 17 -14.641145893916438 119 18 -15.114113509259223 119 19 -7.340005418978038 119 20 -8.98073612523466 119 21 1.3416521700079755 119 22 2.094777518171007 119 23 1.8181223519504641 119 24 1.6259528077955352 119 25 1.7668595615149272 119 26 3.9364288341820526 119 27 4.558351286941992 119 28 4.3980426865012845 119 29 4.411872639165736 119 30 4.842043135820699 119 31 3.182631103765317 119 32 -2.670721759624495 119 33 3.460404920646564 119 34 2.9685066779217806 119 35 2.8573945736710016 119 36 4.213756909871179 119 37 4.608461377412429 119 38 4.876831835710195 119 39 5.519054610483934 119 40 8.924025225206101 119 41 3.9808039061733744 119 42 0.10541847698440421 119 43 -6.186025456587828 119 44 -5.17005226832417 119 45 -4.153460478095511 119 46 -15.725332820156527 119 47 -15.234323264817405 119 48 -14.241338810903834 119 49 -13.23148249436322 119 50 -13.440408931365106 119 51 -11.712822909822044 119 52 -11.385497833751657 119 53 -11.830952657234896 119 54 -10.711753055954958 119 55 -10.411055058189078 119 56 -8.354533809773177 120 1 0.6620514969516051 120 2 1.608504640939401 120 3 2.182482363249953 120 4 0.9041619400835472 120 5 2.75572227619908 120 6 1.3751955138455898 120 7 1.4621255090138092 120 8 1.4687948126905397 120 9 0.8516207040756001 120 10 0.34006406837045944 120 11 -5.656997520758594 120 12 -10.615796203966923 120 13 -9.608271473375261 120 14 -10.90249996746433 120 15 -14.736323724897055 120 16 -15.541175238873489 120 17 -15.476562560583105 120 18 -15.65091906481478 120 19 -7.655283196755814 120 20 -13.15226390301244 120 21 -4.873625607769803 120 22 -2.703833592940107 120 23 -3.3152109813828687 120 24 -7.946963858871129 120 25 -3.3678626607072957 120 26 0.814901056404274 120 27 0.5146012869419891 120 28 1.1029037976123988 120 29 1.0938170836101833 120 30 1.0996820247095904 120 31 -0.06598000734579301 120 32 -10.281832870735606 120 33 -0.48403952379787896 120 34 0.3448955668106706 120 35 -1.2794109818845527 120 36 -0.25638197901771065 120 37 -0.14292751147646143 120 38 0.5497485023768611 120 39 0.4197490549283813 120 40 2.3205530029838783 120 41 -4.731696093826624 120 42 -6.150831523015597 120 43 -13.72213656769894 120 44 -11.620746712768614 120 45 -13.524293811428844 120 46 -16.694777264600972 120 47 -16.618351042595183 120 48 -15.652449922014945 120 49 -16.799538049918777 120 50 -15.623047820253994 120 51 -14.12323957648871 120 52 -13.591053389307213 120 53 -14.138591546123784 120 54 -13.87147527817718 120 55 -13.527721724855745 120 56 -11.385783809773177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1051.0 5 260.0 6 40.0 7 69.0 8 198.0 9 185.0 10 182.0 11 177.0 12 139.0 13 158.0 14 196.0 15 258.0 16 247.0 17 368.0 18 435.0 19 560.0 20 618.0 21 749.0 22 894.0 23 1054.0 24 1147.0 25 1248.0 26 1323.0 27 1425.0 28 1637.0 29 1883.0 30 2243.0 31 2452.0 32 3041.0 33 3492.0 34 4036.0 35 4814.0 36 5967.0 37 6091.0 38 4470.0 39 1806.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.10767942017374 0.7775936481343216 0.08923205798262707 0.02549487370932202 8 99.45003915284177 0.41520222898038717 0.06737930908892248 0.06737930908892248 9 98.7653196875057 0.7320670879391037 0.34418079507584726 0.15843242947935826 10 59.016990512264854 23.40611512756542 10.862637262578989 6.714257097590734 11 41.937246189426915 30.267878280188665 13.277366015333344 14.51750951505108 12 37.237084114872616 12.871269098392002 19.075628721796296 30.816018064939087 13 26.520353337583096 13.080775885620618 22.777524815590567 37.62134596120572 14 23.6172576444663 16.15946384017192 33.154856216649364 27.068422298712413 15 31.714483866257847 15.27960286173164 27.553292451452766 25.452620820557748 16 38.29868639204183 16.869204000947402 22.28760908776213 22.54450051924864 17 31.021798117021472 18.589405058911368 27.383315426219657 23.005481397847504 18 29.555842878735454 19.66565294192632 26.762333145156887 24.01617103418134 19 34.921618665694496 23.751367116296027 18.507109004739338 22.819905213270143 20 29.40211447320452 28.574553408676636 18.800583302952973 23.22274881516588 21 29.01316628120846 22.91442827745707 25.456631398758034 22.61577404257644 22 26.352594103399923 20.181013603336186 25.18529309999454 28.281099193269355 23 28.74911223207619 20.14823447999563 23.234935261231403 27.867718026696775 24 31.041829803507365 19.80587474732759 26.405404913226377 22.746890535938665 25 33.14090073027262 18.994372712207024 21.518457139735208 26.346269417785145 26 23.785762415545157 17.471909089253522 27.00187576259766 31.740452732603668 27 23.51622311337951 15.242435289828654 27.72511848341232 33.51622311337951 28 22.95868130265125 23.53670544473214 27.028554757302796 26.476058495313808 29 32.35754881958612 19.329277178665112 26.326143981346544 21.987030020402216 30 26.512212447407247 16.756825674371164 35.42793654262973 21.30302533559186 31 27.07153135758393 20.67068616914698 32.14084045246726 20.116942020801837 32 35.515091863517064 14.738261883931175 29.91943715368912 19.827209098862642 33 29.714744291238055 15.453782738746627 32.003720726636026 22.827752243379294 34 23.180219740174554 21.07756500191316 38.95195233496711 16.790262922945175 35 22.286796990289492 20.452185319462917 41.51651514875476 15.74450254149283 36 22.657801256945078 27.70744147918754 32.92103105929502 16.713726204572367 37 24.831235176062762 19.98905309250137 37.195767195767196 17.983944535668673 38 30.59882754716292 21.45661741877751 30.976861405847657 16.96769362821192 39 26.84528260592022 18.862280909737546 31.04561454704627 23.246821937295955 40 18.809885861898394 13.982375464888335 32.06676071304253 35.14097796017075 41 15.422173274596181 14.341042584434655 30.618575624082233 39.61820851688693 42 23.776963331692286 18.151815181518153 33.30051237167916 24.770709115110407 43 16.658151162366337 13.661910149264624 38.859895624247294 30.820043064121748 44 18.77434575080924 14.215175289405826 33.46317733764928 33.54730162213566 45 23.15384194295933 17.0921299928836 37.99244566903271 21.761582395124353 46 20.61468308253534 20.069311445508436 33.84587323301413 25.47013223894209 47 18.35753192574645 21.472683220082704 36.398083544349916 23.771701309820926 48 20.778770745942 21.557541491884006 37.91902243297465 19.744665329199343 49 19.35336309741638 17.492497987264876 31.804874478518624 31.349264436800116 50 24.751572613729603 16.243960251618198 35.521925426201115 23.48254170845109 51 20.34235814242391 21.887902371295993 37.33055646899777 20.43918301728233 52 19.686115819981406 17.33289586409289 39.682105685277335 23.298882630648365 53 19.614458710242193 16.207688940764516 42.23993288590604 21.937919463087248 54 18.226430239346175 19.74423525977817 36.28137769994162 25.747956800934034 55 19.8211352436576 21.332359919693374 35.19985398795401 23.646650848695018 56 23.8672017511857 18.004377964246626 33.017147026632614 25.111273257935064 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.0 11 0.0 12 7.0 13 34.0 14 54.0 15 70.0 16 86.0 17 116.5 18 147.0 19 189.5 20 232.0 21 258.5 22 306.5 23 328.0 24 434.5 25 541.0 26 689.0 27 837.0 28 1022.0 29 1355.5 30 1504.0 31 1574.0 32 1644.0 33 1638.5 34 1633.0 35 1452.5 36 1272.0 37 1092.5 38 771.5 39 630.0 40 566.5 41 503.0 42 543.5 43 584.0 44 609.5 45 635.0 46 752.5 47 1446.0 48 2022.0 49 2061.0 50 2100.0 51 1802.5 52 1505.0 53 1589.0 54 1825.0 55 1977.0 56 2063.5 57 2150.0 58 2384.5 59 2619.0 60 3006.5 61 3394.0 62 3923.0 63 4460.5 64 4469.0 65 4166.5 66 3864.0 67 3399.5 68 2935.0 69 2593.5 70 2252.0 71 2006.0 72 1572.5 73 1385.0 74 1221.5 75 1058.0 76 856.5 77 655.0 78 509.0 79 289.0 80 215.0 81 154.0 82 93.0 83 59.0 84 25.0 85 23.0 86 21.0 87 11.5 88 2.5 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.014568499262469723 14 0.007284249631234862 15 0.22034855134485462 16 0.04734762260302661 17 0.0 18 0.0 19 0.09651630761386193 20 0.09651630761386193 21 0.0 22 0.0 23 0.0 24 0.0 25 0.003642124815617431 26 0.003642124815617431 27 0.09651630761386193 28 0.1292954309544188 29 0.030958060932748166 30 0.018210624078087156 31 0.02549487370932202 32 0.08923205798262707 33 0.15479030466374083 34 0.05463187223426147 35 0.043705497787409175 36 0.03277912334055688 37 0.1875694280042977 38 0.28408573561815964 39 0.15296924225593211 40 0.6009505945768762 41 0.7885200225811739 42 0.1274743685466101 43 0.20213792726676744 44 0.422486478611622 45 0.2003168648589587 46 0.16025349188716698 47 0.03642124815617431 48 0.1511481798481234 49 0.4752972884380748 50 0.12383224373099266 51 0.31868592136652524 52 0.09469524520605321 53 0.14750605503250597 54 0.17664305355744542 55 0.22399067616047202 56 0.16935880392621055 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 54913.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.79174330304299 #Duplication Level Percentage of deduplicated Percentage of total 1 86.56875428049615 62.149217853695845 2 7.716307739137052 11.079343689108226 3 2.55941962813586 5.512355908436982 4 1.1287826903076885 3.2414910858995136 5 0.5808791822032824 2.085116456940979 6 0.32468356035816653 1.3985759291970936 7 0.24351267026862491 1.223753938047457 8 0.1902442736473632 1.0926374446852294 9 0.11414656418841793 0.7375302751625298 >10 0.5377571468432134 6.641414601278386 >50 0.015219541891789057 0.7284249631234863 >100 0.017756132207087234 2.562234807786863 >500 0.002536590315298176 1.5479030466374084 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 850 1.5479030466374084 No Hit ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 262 0.4771183508458835 No Hit ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 230 0.41884435379600454 No Hit ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 229 0.41702329138819594 No Hit ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 214 0.38970735527106515 No Hit ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 175 0.31868592136652524 No Hit ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 173 0.3150437965509078 No Hit ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 124 0.22581173856828074 No Hit ACGAGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 83 0.1511481798481234 No Hit ACGAGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 76 0.1384007429934624 No Hit ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 67 0.12201118132318393 No Hit ACGAGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC 66 0.12019011891537523 No Hit ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 56 0.10197949483728808 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.005463187223426147 0.0 0.0 0.0 0.0 13 0.007284249631234863 0.0 0.0 0.0 0.0 14 0.009105312039043578 0.0 0.0 0.0 0.0 15 0.009105312039043578 0.0 0.0 0.0 0.0 16 0.010926374446852294 0.0 0.0 0.0 0.0 17 0.01274743685466101 0.0 0.0 0.0 0.0 18 0.014568499262469725 0.0 0.0 0.0 0.0 19 0.01638956167027844 0.0 0.0 0.0 0.0 20 0.018210624078087156 0.0 0.0 0.0 0.0 21 0.018210624078087156 0.0 0.0 0.0 0.0 22 0.020031686485895872 0.0 0.0 0.0 0.0 23 0.02549487370932202 0.0 0.0 0.0 0.0 24 0.03277912334055688 0.0 0.0 0.0 0.0 25 0.03642124815617431 0.0 0.0 0.0 0.0 26 0.043705497787409175 0.0 0.0 0.0 0.0 27 0.043705497787409175 0.0 0.0 0.0 0.0 28 0.04552656019521789 0.0 0.0 0.0 0.0 29 0.04916868501083532 0.0 0.0 0.0 0.0 30 0.05463187223426147 0.0 0.0 0.0 0.0 31 0.0582739970498789 0.0 0.0 0.0 0.0 32 0.060095059457687616 0.0 0.0 0.0 0.0 33 0.06191612186549633 0.0 0.0 0.0 0.0 34 0.06373718427330505 0.0 0.0 0.0 0.0 35 0.06555824668111376 0.0 0.0 0.0 0.0 36 0.07102143390453991 0.0 0.0 0.0 0.0 37 0.07284249631234863 0.0 0.0 0.0 0.0 38 0.07284249631234863 0.0 0.0 0.0 0.0 39 0.07284249631234863 0.0 0.0 0.0 0.0 40 0.07284249631234863 0.0 0.0 0.0 0.0 41 0.07284249631234863 0.0 0.0 0.0 0.0 42 0.07284249631234863 0.0 0.0 0.0 0.0 43 0.07648462112796606 0.0 0.0 0.0 0.0 44 0.07648462112796606 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTCA 45 1.1641532E-10 50.60148 41 TCGTCTC 30 9.538635E-7 50.55463 40 TGCTCGT 30 9.72339E-7 50.41459 37 GTCGTCT 15 0.008178114 50.32166 43 CCAAAAC 25 1.9843206E-5 50.32166 43 TCGTCTT 15 0.008178114 50.32166 44 CGACTCC 25 1.9843206E-5 50.32166 44 CCGAATC 15 0.008178114 50.32166 43 ATGCTCG 30 9.911182E-7 50.275326 36 AGTTGTC 15 0.008207987 50.275326 42 GCGTTCA 15 0.008207987 50.275326 45 CGTTCAG 15 0.008237942 50.229073 46 GTTCAGC 15 0.008237942 50.229073 47 AACGATT 55 0.0 50.22907 47 TATGCCC 15 0.008267977 50.182907 35 ACGATTA 50 7.2759576E-12 50.182907 48 ATATGCC 50 7.2759576E-12 50.090828 34 TAGAGCG 35 5.0924427E-8 50.09082 34 ATCCCAT 15 0.008419379 49.953342 14 TGACACG 15 0.008419379 49.953342 14 >>END_MODULE