FastQCFastQC Report
Fri 17 Jun 2016
SRR1526224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254773
Sequences flagged as poor quality0
Sequence length56
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC10280.40349644585572253No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC9750.38269361353047615No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG9510.3732734630435721No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC6820.26768927633618944No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC6230.24453140638921708No Hit
CGTATTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2930.11500433719428668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT301.009581E-650.24786844
GTAAGCC150.00826038750.24786835
AACGATT507.2759576E-1250.1683547
ACGAAAC150.00831227450.16834648
CAACGAT507.2759576E-1250.13859646
AAGCTAT252.0998508E-549.93128610
ATCATCG150.00846938949.93128210
ACGAATC150.00846938949.93128210
GTAGCGA150.0084825849.9116329
ACTAGAT204.2471607E-449.83316818
TCTGTTA150.00854213349.8233821
GGATACG204.2677944E-449.784268
TAGATAC204.2677944E-449.7842620
GGGTAAT150.008568749.7842568
CGTATTA850.049.7744831
GTATTAG850.049.7744832
CGTATTG258650.048.783421
GTATTGG257250.048.778032
TATTGGG249850.048.7684333
ATTGGGA88550.048.734594