##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526224_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 254773 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.383733755146736 39.0 38.0 40.0 35.0 40.0 2 37.9212750173684 39.0 38.0 40.0 35.0 40.0 3 37.90256816852649 39.0 38.0 40.0 35.0 40.0 4 37.042006021046184 39.0 38.0 40.0 33.0 40.0 5 38.01223049538216 39.0 38.0 40.0 35.0 40.0 6 38.10211443127804 39.0 38.0 40.0 35.0 40.0 7 38.116244657008394 39.0 38.0 40.0 35.0 40.0 8 38.01472291019849 39.0 38.0 40.0 35.0 40.0 9 38.01689739493588 39.0 38.0 40.0 35.0 40.0 10 37.49129224839367 39.0 38.0 40.0 33.0 40.0 11 36.90334140587896 39.0 36.0 40.0 32.0 40.0 12 34.89368967669259 38.0 35.0 40.0 23.0 40.0 13 34.59702951254646 38.0 33.0 40.0 23.0 40.0 14 34.69096803821441 38.0 33.0 40.0 23.0 40.0 15 34.63189584453612 38.0 33.0 39.0 23.0 40.0 16 33.965447673026574 38.0 33.0 39.0 22.0 40.0 17 33.86856535033147 38.0 32.0 39.0 22.0 40.0 18 33.36128239648628 38.0 31.0 39.0 19.0 40.0 19 34.27933101231292 38.0 32.0 39.0 24.0 40.0 20 34.28057133212703 38.0 32.0 39.0 24.0 40.0 21 35.0259211140898 38.0 34.0 39.0 26.0 40.0 22 34.66609884092898 38.0 33.0 39.0 25.0 40.0 23 35.21975248554595 38.0 34.0 39.0 27.0 40.0 24 34.823709733762996 38.0 33.0 39.0 25.0 40.0 25 35.11058864165355 38.0 34.0 39.0 27.0 40.0 26 35.66595361360898 38.0 35.0 39.0 28.0 40.0 27 35.554340530589975 38.0 35.0 39.0 28.0 40.0 28 35.502843707928236 38.0 35.0 39.0 28.0 40.0 29 35.31661518292755 38.0 35.0 39.0 27.0 40.0 30 35.17034379624214 38.0 35.0 39.0 27.0 40.0 31 35.422501599463054 38.0 35.0 39.0 29.0 40.0 32 35.10620827167714 38.0 35.0 39.0 27.0 40.0 33 35.27105305507256 38.0 35.0 39.0 28.0 40.0 34 35.06909680382144 38.0 35.0 39.0 27.0 40.0 35 34.95855918798303 38.0 35.0 39.0 27.0 40.0 36 34.3535303976481 38.0 33.0 39.0 27.0 40.0 37 34.16444835206242 37.0 33.0 39.0 27.0 40.0 38 33.868844029783375 37.0 33.0 39.0 26.0 40.0 39 33.405678780718524 36.0 32.0 39.0 23.0 40.0 40 30.007430143696546 35.0 25.0 38.0 9.0 40.0 41 27.032150188599264 32.0 16.0 38.0 6.0 39.0 42 30.988534891844896 35.0 29.0 38.0 11.0 39.0 43 29.45318381461144 34.0 26.0 38.0 6.0 39.0 44 29.411534974271213 34.0 26.0 38.0 4.0 39.0 45 30.56526005502938 35.0 29.0 38.0 4.0 39.0 46 30.071165311865858 35.0 27.0 38.0 4.0 40.0 47 29.614099610241272 35.0 27.0 38.0 4.0 40.0 48 29.665270652698677 35.0 27.0 38.0 4.0 40.0 49 27.02165457093177 33.0 15.0 38.0 4.0 40.0 50 29.248880375864005 35.0 26.0 38.0 4.0 40.0 51 29.480329548264532 35.0 27.0 39.0 4.0 40.0 52 29.212024037083992 35.0 26.0 39.0 4.0 40.0 53 28.808343113281236 35.0 25.0 39.0 4.0 40.0 54 27.803774340295085 34.0 18.0 38.0 4.0 40.0 55 28.16909170123993 34.0 23.0 38.0 4.0 40.0 56 27.01472291019849 33.0 17.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.1277999474144167 1 2 0.284207199055011 1 3 0.2768637376906753 1 4 1.2528459353186392 1 5 0.2616485344682289 1 6 0.180007575876445 1 7 0.04064371332041361 1 8 0.07983274178134536 1 9 0.14516238278368832 1 10 0.3427509764400156 1 11 -2.3383951218416854 1 12 -13.20484395068194 1 13 -7.932221736996361 1 14 -4.947005414170164 1 15 -6.057832172272988 1 16 -23.393881608784277 1 17 -4.3341819546490505 1 18 -13.682858603306322 1 19 -5.3893209688936885 1 20 -5.4764496519987595 1 21 -5.778546768183617 1 22 -3.048477941697861 1 23 -2.9184114645307133 1 24 -3.6727464077521645 1 25 -3.2277231548725247 1 26 1.871064819412183 1 27 1.6983756695253405 1 28 1.5300285849402258 1 29 1.7589205376567207 1 30 2.004742751697158 1 31 1.420833214799515 1 32 1.5299812050699444 1 33 1.4161233491344305 1 34 1.3217240489583872 1 35 1.3713709937011345 1 36 1.8826010546971617 1 37 1.846508628429568 1 38 2.091013709281441 1 39 2.624816112687384 1 40 5.3045777239629714 1 41 -8.403150894601087 1 42 -7.727786705195204 1 43 -16.06984613288145 1 44 -21.57921934163007 1 45 -9.270217536674245 1 46 -16.14158529620689 1 47 -22.908410912900862 1 48 -13.048977283133153 1 49 -19.48138574327005 1 50 -11.921826472337049 1 51 -8.736834676438846 1 52 -8.301642526046052 1 53 -8.272565914062671 1 54 -7.342963198477193 1 55 -7.632585356462723 1 56 -2.6583784774286734 2 1 0.7731519974910555 2 2 1.0359861341739602 2 3 1.095526817871523 2 4 1.8975739919393249 2 5 1.167913438415269 2 6 1.0929112939939216 2 7 1.0001240544181016 2 8 0.8732854974615378 2 9 0.9147115920398718 2 10 1.1867293049087024 2 11 0.8331361931395236 2 12 -2.6382936540337774 2 13 -0.8180646565484295 2 14 2.7921731279615045 2 15 2.824376568832335 2 16 2.7653736230972896 2 17 3.3117193406827923 2 18 -1.187542019624832 2 19 2.4700143866071755 2 20 2.669718722064083 2 21 2.480777141085923 2 22 2.8209014817936193 2 23 2.283185284739588 2 24 1.8618498264377266 2 25 2.416938132065418 2 26 2.0386376460590085 2 27 2.2835642461864722 2 28 2.06341342631454 2 29 2.6337750751335847 2 30 2.603485573956263 2 31 1.6862331651991838 2 32 2.0053718077140275 2 33 1.397370606808174 2 34 1.6350550930454517 2 35 1.725927547622426 2 36 1.8567898030517611 2 37 2.0475616817117768 2 38 2.0554063913242118 2 39 2.174235216447471 2 40 5.2484530363497015 2 41 -7.210758823023987 2 42 -5.981425652905006 2 43 -14.358462855825422 2 44 -14.194454275528082 2 45 -5.081154410708468 2 46 -10.129891063199775 2 47 -17.47816615432096 2 48 -7.145736091962011 2 49 -12.145162236526312 2 50 -6.414806117122339 2 51 -1.0751185048216847 2 52 -0.7893187503158785 2 53 -1.004533330457491 2 54 0.020330645358058064 2 55 -0.6094162765489521 2 56 2.6704908256866062 3 1 0.7751469844469057 3 2 1.0819816667672697 3 3 0.9186465000377027 3 4 1.9178478337385698 3 5 1.0005135714144018 3 6 0.9766137851699455 3 7 0.7323695230695293 3 8 0.91946006734576 3 9 0.8375891475840618 3 10 0.9531367937828179 3 11 1.3457370081854734 3 12 3.366463622553262 3 13 2.257182159145465 3 14 3.4358036631977455 3 15 2.5446094878222283 3 16 4.340952936617164 3 17 4.289083911760599 3 18 4.901737909442296 3 19 3.8626431655728553 3 20 3.4281036351883643 3 21 3.2200221152534 3 22 3.336580033126168 3 23 3.0142968505485825 3 24 3.3205945192608937 3 25 2.8617946464651283 3 26 2.435946971344187 3 27 2.5482836691748645 3 28 2.4697172440716315 3 29 2.507715771162367 3 30 2.2088959103758583 3 31 1.8622012454078885 3 32 1.7095425240337008 3 33 1.901505515669669 3 34 1.543021399932421 3 35 1.1317027790920662 3 36 2.122327682227173 3 37 2.2739159709337287 3 38 2.5757825401468324 3 39 2.8653021081895886 3 40 6.675704217143263 3 41 7.08497667921824 3 42 3.604495195561242 3 43 -0.8883279810662685 3 44 1.8950643699269278 3 45 4.088496210806699 3 46 2.118527440730521 3 47 -3.1354035236663655 3 48 1.945999205666169 3 49 4.061387118086881 3 50 2.086847846422259 3 51 3.410132232641441 3 52 3.561875684182052 3 53 3.6650490863754257 3 54 4.7863630084797855 3 55 3.603169192443495 3 56 3.782960346719225 4 1 0.9498295546662234 4 2 1.2552790888526886 4 3 1.193264943028602 4 4 2.0002128459227393 4 5 1.2893426082234285 4 6 1.1420107327138709 4 7 1.0171970208250727 4 8 1.0404296710394902 4 9 1.1254177996297159 4 10 1.0371691139059394 4 11 1.8342838851894498 4 12 4.178109127234549 4 13 4.148191521720818 4 14 3.8787945571313145 4 15 3.63230988541104 4 16 4.8552789594464585 4 17 4.863407369500756 4 18 5.1960049352214455 4 19 4.287447577526173 4 20 3.829591386887742 4 21 3.068604903505289 4 22 3.4605764420013827 4 23 3.1290845894225043 4 24 3.450054567997583 4 25 2.87151633429378 4 26 2.244719576192203 4 27 2.165444115500371 4 28 2.3154784342729897 4 29 2.460800290849427 4 30 2.273561807515783 4 31 1.8649715416756862 4 32 2.053700533524534 4 33 1.8561290888388342 4 34 1.8155108189397353 4 35 2.0599059341517005 4 36 3.0153891161119972 4 37 2.9654640022278187 4 38 3.134664161283162 4 39 3.6811800099466723 4 40 7.396212104792362 4 41 9.656427237125126 4 42 5.613036942386941 4 43 7.053701004440978 4 44 6.580430536401213 4 45 5.604771701379999 4 46 5.456118822031019 4 47 4.766635722197432 4 48 5.082461947746459 4 49 8.167889619048793 4 50 5.0605813336609415 4 51 4.650968451700056 4 52 4.890207093698532 4 53 4.876207224120712 4 54 5.495148438773487 4 55 3.8067603172350815 4 56 4.417127334022034 5 1 0.8252087255458989 5 2 1.240381886291182 5 3 1.18692015030868 5 4 2.1034484374293143 5 5 1.187640552274658 5 6 1.0227909821240502 5 7 0.9035552951762682 5 8 0.8131967758591685 5 9 1.0263025316822976 5 10 1.026274270629905 5 11 1.7366010405652546 5 12 4.045770064207922 5 13 3.8497166312590707 5 14 3.9077800643776897 5 15 4.119359093365205 5 16 4.684079094262771 5 17 4.615977305463005 5 18 4.785270186316822 5 19 3.8509123567644608 5 20 3.7549284276696753 5 21 2.7964781761712842 5 22 3.484784396163832 5 23 2.9537391226210232 5 24 3.3905163138688366 5 25 2.86652813072115 5 26 2.1525810524308326 5 27 2.2908232863800464 5 28 2.240520564370726 5 29 2.6676337927031497 5 30 2.4504408098750687 5 31 2.2744002575503046 5 32 2.1982404728571936 5 33 2.137322213206879 5 34 1.4185273339380444 5 35 2.012105125253825 5 36 2.576073308899325 5 37 3.0282378478631387 5 38 3.438219941532573 5 39 3.7230489010824996 5 40 7.527388377121312 5 41 9.453073681344879 5 42 6.059792924860822 5 43 6.985703026685666 5 44 7.135983283283583 5 45 5.66677877923642 5 46 5.496117136827113 5 47 5.04193063288162 5 48 5.434079743499744 5 49 8.36648247378421 5 50 6.015476851018541 5 51 5.334765216108547 5 52 5.131823204247905 5 53 5.262068363318555 5 54 5.8448198240111005 5 55 5.423030960982977 5 56 4.915197775545458 6 1 0.9741935336674317 6 2 1.32418921583254 6 3 1.0538922145321692 6 4 2.332370606051967 6 5 1.3120015573866155 6 6 1.2588680935071608 6 7 0.9087122539205978 6 8 1.0568428266907617 6 9 0.9517201283415204 6 10 1.3856143959089025 6 11 2.0130338291029446 6 12 4.250734424493039 6 13 4.2012628188895675 6 14 4.0749627269163895 6 15 4.229797209860273 6 16 4.800664161582233 6 17 5.06294072975561 6 18 5.216312737976409 6 19 4.334820485499428 6 20 3.8882623609982048 6 21 3.3767935336484243 6 22 3.8048668355043205 6 23 3.355045912166709 6 24 3.745455474355552 6 25 3.2716688895950767 6 26 2.5224150937585037 6 27 2.7935232647802195 6 28 2.785196206965587 6 29 2.9106948482147104 6 30 2.448856822546965 6 31 2.0338771817349084 6 32 2.10285923590709 6 33 2.0730627272827675 6 34 2.198263096051953 6 35 2.1256752166930966 6 36 2.7777976951042334 6 37 2.8046976361848337 6 38 3.0241692539380765 6 39 3.550727279655476 6 40 7.432565135707243 6 41 3.9506077010727196 6 42 4.990898476016412 6 43 6.73912299932589 6 44 6.13901625901978 6 45 4.645853946635075 6 46 5.8236335166960735 6 47 6.086858273460496 6 48 5.113556307687233 6 49 7.632133348577213 6 50 5.848969183079888 6 51 5.341290163908969 6 52 5.170549894434384 6 53 5.409325185263981 6 54 6.078160957282034 6 55 5.125448341643931 6 56 4.9539604654439415 7 1 0.8447112277537272 7 2 1.101435757353883 7 3 1.2176457905795033 7 4 2.0757769625985816 7 5 1.1451030191572045 7 6 1.0333517710554574 7 7 0.9390645680641114 7 8 1.0186721953469515 7 9 0.8318294672136446 7 10 0.9717040616219563 7 11 1.9213596511039626 7 12 3.791354403330679 7 13 4.15899687853608 7 14 3.9805648745042745 7 15 4.0589013365209325 7 16 4.48853891174879 7 17 4.86858990481538 7 18 5.127999064738965 7 19 3.55366478537794 7 20 3.9250417629655203 7 21 2.765642144378525 7 22 3.3419009696109896 7 23 2.935266941284553 7 24 3.219188671831745 7 25 3.1001165911273887 7 26 2.55429580073816 7 27 2.2881738898537023 7 28 1.8193401110295468 7 29 2.715138944307512 7 30 2.572754616174741 7 31 1.898075559287129 7 32 2.2370246942289924 7 33 2.0434158252174797 7 34 1.9252980139498206 7 35 2.0603462203377276 7 36 2.038760974486607 7 37 2.4756914834239296 7 38 2.4741339833341627 7 39 3.120053611120767 7 40 6.018409866670915 7 41 0.2651137166701645 7 42 3.7517637814990366 7 43 5.951334466191117 7 44 5.764589406333332 7 45 4.220539450416869 7 46 4.867584608125316 7 47 4.805692775949037 7 48 5.205054132666653 7 49 7.443719741962013 7 50 4.785384122140115 7 51 5.1369068116340095 7 52 4.891353672307961 7 53 5.111494327404515 7 54 6.148065334755881 7 55 4.948899374288835 7 56 5.014439753761533 8 1 0.8161374430672979 8 2 0.9336626983508722 8 3 1.0468834106677463 8 4 1.8259315826455804 8 5 0.984947574469949 8 6 0.912562590335547 8 7 0.6083219795183652 8 8 0.6971449616026746 8 9 0.8692784425981372 8 10 0.8436271226865628 8 11 1.81177526979031 8 12 3.784180033149667 8 13 3.9896776190630234 8 14 4.070646513889088 8 15 4.15252892898372 8 16 4.73826059566418 8 17 4.578697932688215 8 18 4.9024393401688044 8 19 3.81386970848024 8 20 3.91104349471005 8 21 3.2111437318110063 8 22 2.937600470703643 8 23 2.685710185419296 8 24 3.1429403726522622 8 25 3.0760577118134904 8 26 2.4680590465221854 8 27 2.3574849029242486 8 28 2.2964254200634784 8 29 2.4468834707578466 8 30 2.7692045398955187 8 31 1.93680272706019 8 32 2.2765868102558713 8 33 1.2918180847317657 8 34 1.1791325911155326 8 35 1.5750454328062844 8 36 2.0097336376623005 8 37 2.3419451427650415 8 38 2.2782509997857403 8 39 2.740822175834417 8 40 4.6624539024603 8 41 -0.7938583692573467 8 42 3.792171153782874 8 43 5.445005352267064 8 44 5.349764436226547 8 45 3.9127734008559933 8 46 4.605493320449565 8 47 4.592873743252046 8 48 4.474474410570423 8 49 7.373120228499765 8 50 4.517891440822346 8 51 4.304360322539882 8 52 4.181431188491526 8 53 4.446164265144176 8 54 5.130016021268887 8 55 4.767313591086754 8 56 4.087410107944521 9 1 0.3193947720575281 9 2 0.5302435673977328 9 3 0.6797952073082243 9 4 1.26821511929586 9 5 0.6081831879335766 9 6 0.2614480728673243 9 7 0.296225153817403 9 8 0.39155214901618507 9 9 0.3125732415166169 9 10 0.6281324557644226 9 11 1.4371824359140888 9 12 3.294250076367824 9 13 3.441200899384924 9 14 3.6463421558809586 9 15 3.541630715192227 9 16 4.326463955183883 9 17 3.8617023396627346 9 18 4.325359865599388 9 19 3.426151754849272 9 20 3.2979630833924247 9 21 2.46124485803783 9 22 2.463105569594447 9 23 2.3923696135770953 9 24 2.8364427464573723 9 25 2.4077198011877883 9 26 1.5122671749418544 9 27 1.4140943002119357 9 28 1.5911817590811737 9 29 1.7970037577434326 9 30 1.6297993750065487 9 31 1.1881599474727835 9 32 1.5149679390374615 9 33 1.259681234334714 9 34 1.021620509192644 9 35 1.0818155675702883 9 36 1.4864320260295898 9 37 1.5411957441999675 9 38 1.9598417685579577 9 39 2.42860825660766 9 40 4.8859535086002595 9 41 2.724429510498247 9 42 3.595645638039116 9 43 5.526704698672294 9 44 4.793815020629683 9 45 3.6272780952418877 9 46 3.824149512568102 9 47 4.411591675593282 9 48 4.135744130184648 9 49 6.443450858037139 9 50 3.7595298985941596 9 51 3.372593913478969 9 52 3.2840689862816532 9 53 2.943896908690192 9 54 4.257211524877498 9 55 3.07109677645645 9 56 2.9665163649967425 10 1 0.6527281053908638 10 2 0.90991677001211 10 3 1.0658736386807703 10 4 1.943836034328534 10 5 0.9348498546002375 10 6 0.826938268945753 10 7 0.6899506440134786 10 8 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2.1207752416456387 114 30 1.892205572053264 114 31 1.3754027458266975 114 32 1.7805210738716397 114 33 1.5125255819502996 114 34 1.9251547624000978 114 35 1.7544130016056343 114 36 2.7202737108516644 114 37 2.6922487579980796 114 38 2.95015888305808 114 39 3.718610677329032 114 40 7.230422160258456 114 41 6.107992812362202 114 42 2.1594316462695744 114 43 -0.8019082279798511 114 44 -7.373335790311973 114 45 1.8093163426396117 114 46 -3.6942479698816655 114 47 -3.1323444173624218 114 48 -3.44196102595188 114 49 -17.469040064257705 114 50 -4.201254415131331 114 51 -3.6368468998834125 114 52 -3.1187390505133123 114 53 -4.892946470636847 114 54 -8.561355408068522 114 55 -4.4454851649620934 114 56 -0.3594337681429316 115 1 -0.12299205921819123 115 2 0.22353816918906233 115 3 0.19068845349558927 115 4 1.1332599026412922 115 5 0.19254532785127054 115 6 -0.06450206027235339 115 7 -0.06288886215936174 115 8 -0.1659399836393476 115 9 -0.12460903880291596 115 10 0.22532441896945699 115 11 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115 50 -4.238291452168365 115 51 -3.3817028669616036 115 52 -3.032319297426895 115 53 -3.4855390632294423 115 54 -9.248598206422432 115 55 -4.268530432451804 115 56 -2.4047012578548674 116 1 0.20034715300990769 116 2 0.6619680520633935 116 3 0.5297197925269259 116 4 1.648341528834031 116 5 0.6118461915965767 116 6 0.42466720667469104 116 7 0.3480458821087211 116 8 0.3330877376105974 116 9 0.14414689661968083 116 10 0.5835299993972569 116 11 -1.6140996153679623 116 12 -12.97490886926171 116 13 -9.300988283980729 116 14 -3.125186968589343 116 15 -6.117745855949046 116 16 -2.9589271243248447 116 17 -1.8008703817335174 116 18 -7.437981368330078 116 19 -2.4272203632287273 116 20 -3.0138231695702977 116 21 2.134411560616293 116 22 2.4187544958316067 116 23 1.9900497112571927 116 24 2.121690178763629 116 25 2.0783008541217782 116 26 1.7205915303250308 116 27 2.0606503585327047 116 28 1.9654545451186607 116 29 2.115936459029072 116 30 2.4235219922585713 116 31 1.4647167240295644 116 32 2.0315951027234505 116 33 1.649503830039663 116 34 1.5096977913875733 116 35 1.8147395063765828 116 36 2.6088912661358847 116 37 2.53609626851226 116 38 2.5209995650098733 116 39 3.516376697317277 116 40 6.890622042680562 116 41 5.39393320941371 116 42 0.26597530836878747 116 43 -8.741807834546123 116 44 -6.797113658534286 116 45 0.23404387847826058 116 46 -3.186085350607936 116 47 -2.249831868183204 116 48 -3.236697154021339 116 49 -7.708491970376279 116 50 -2.4417916556685704 116 51 -1.2930672227704036 116 52 -0.5554052649573045 116 53 -2.1457389456515443 116 54 -8.300965592123152 116 55 -1.347850289549438 116 56 -0.6773463082776949 117 1 -0.46139340528237227 117 2 -0.10284185067754947 117 3 -0.1857027159990281 117 4 1.0832284809623616 117 5 -0.13401714047365232 117 6 -0.2897448180821627 117 7 -0.3531035431786407 117 8 -0.20524749037980428 117 9 -0.2813930806444276 117 10 0.1987883043107388 117 11 -2.533260913839605 117 12 -8.380997033970562 117 13 -11.69368753874895 117 14 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0.4715796595194419 118 35 0.5390984807355537 118 36 0.8409196544142716 118 37 1.2462611036770923 118 38 1.1623686126289172 118 39 1.8809600306506056 118 40 6.021574423632941 118 41 4.034157568388068 118 42 -1.2049496000561213 118 43 -8.389816076304363 118 44 -9.159178676849304 118 45 -1.4845916526572722 118 46 -9.392587182109768 118 47 -10.349992124593463 118 48 -10.503524077098263 118 49 -10.808766695651004 118 50 -6.914548065924979 118 51 -4.5394042191074 118 52 -4.489127792429834 118 53 -3.793060374222975 118 54 -10.881093797251355 118 55 -3.4448054177545657 118 56 -1.5307116928930782 119 1 -0.5168497694271892 119 2 0.004370918483722619 119 3 -0.2354800731096205 119 4 0.8277807213736637 119 5 -0.14873093142595906 119 6 -0.2132072914618135 119 7 -0.30489651755857494 119 8 -0.24560285854724384 119 9 -0.29858068147907346 119 10 -0.14513580350435262 119 11 -4.200372149238753 119 12 -11.493759741824373 119 13 -12.60439375769188 119 14 -12.734141333002224 119 15 -10.97375302199988 119 16 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-12.665170528380488 119 55 -9.268943752127619 119 56 -6.713864375363446 120 1 -1.1401428001968057 120 2 -0.7877132877913624 120 3 -1.0692130665215416 120 4 0.354221390783259 120 5 -1.0315085269026483 120 6 -1.0140251221910503 120 7 -0.8727083155240933 120 8 -0.9244023638101382 120 9 -0.8771959224109978 120 10 -0.7352360130103719 120 11 -3.268019876224642 120 12 -13.47066359623031 120 13 -10.898989512039762 120 14 -14.294532074350634 120 15 -13.276779500531823 120 16 -18.280869151469183 120 17 -15.286546555270963 120 18 -17.100836817312697 120 19 -7.966401798363918 120 20 -14.717564514930139 120 21 -5.574136153711766 120 22 -3.988347073119673 120 23 -3.789069050520528 120 24 -9.70462416200158 120 25 -3.2738133130212894 120 26 0.4721636576197028 120 27 0.2720035525448523 120 28 0.413124408973502 120 29 0.28695426588502926 120 30 0.6873006028579951 120 31 -1.1114546960724923 120 32 -11.824107961072102 120 33 -0.38297621400107573 120 34 -1.3620547335441415 120 35 -1.0110666720307506 120 36 0.4810672596991665 120 37 0.6198160169718889 120 38 0.30107113120860873 120 39 1.0066051586439997 120 40 4.271574423632941 120 41 1.5695879977852591 120 42 -3.643275363887664 120 43 -8.74574609281964 120 44 -8.142431154140802 120 45 -6.043582156373208 120 46 -15.684804357997463 120 47 -14.16602742599726 120 48 -11.550360369418659 120 49 -17.465971485081226 120 50 -10.793418833885344 120 51 -9.36702601927255 120 52 -10.017642449737842 120 53 -9.663492868029746 120 54 -13.115507504930958 120 55 -10.161439397936235 120 56 -6.867081428648653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 539.0 5 77.0 6 85.0 7 366.0 8 582.0 9 601.0 10 425.0 11 365.0 12 309.0 13 308.0 14 399.0 15 509.0 16 737.0 17 942.0 18 1130.0 19 1444.0 20 1650.0 21 2191.0 22 2745.0 23 3241.0 24 3935.0 25 4636.0 26 5209.0 27 5743.0 28 6693.0 29 7933.0 30 9230.0 31 11125.0 32 12655.0 33 14528.0 34 17099.0 35 20420.0 36 25872.0 37 33731.0 38 34714.0 39 22576.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.63536167490275 0.2563065944978471 0.06908110357062953 0.039250627028766784 8 99.37787756159405 0.45569977980398235 0.07889376032782124 0.08752889827414993 9 96.8874252766188 1.9601763138166133 0.6162348443516384 0.5361635652129543 10 48.82660250497502 35.68706260082505 7.84973289948307 7.636601994716866 11 36.5702017089723 28.594081790456606 18.393628838220693 16.442087662350406 12 36.377088623990765 15.234345868675254 22.209574797957398 26.17899070937658 13 26.227789860477536 15.502147405447422 25.607515526487283 32.662547207587764 14 23.996844224813657 18.882447374308693 29.638773643782063 27.481934757095587 15 28.670511589846885 17.47171384959164 27.550867861582766 26.30690669897871 16 33.66037972993142 19.361984587709443 24.1003605627607 22.87727511959843 17 30.85111393716735 21.024680891149732 24.31193380748277 23.812271364200146 18 29.196710694169138 22.175730575236003 24.279630247482974 24.34792848311189 19 28.823002451750803 25.781495567988937 21.722354938077576 23.673147042182688 20 28.594215311117573 26.429867915484767 22.00727604170759 22.96864073169007 21 28.572886451861066 23.42124165433543 24.966146334187687 23.039725559615814 22 26.84782139394677 22.603258587055926 25.848107923524076 24.700812095473225 23 28.043002986972716 22.104383117520303 25.9905876996385 23.86202619586848 24 29.09295725999223 21.65025336279747 25.76411158168252 23.492677795527783 25 28.086434291097508 21.510441199560372 25.237478411053544 25.165646098288587 26 24.624044655369364 20.580488398475374 29.297627879772797 25.497839066382465 27 23.283629005324062 18.745407752303493 30.120822773619377 27.85014046875307 28 23.275116721424865 22.806266014808294 29.817804536808513 24.100812726958328 29 26.823052668365605 20.896143621720746 30.466605672461117 21.814198037452538 30 24.905781853585 19.098803429540528 33.99822555824252 21.99718915863195 31 25.82976718621177 19.90341957520317 33.9990577519532 20.267755486631856 32 29.063029155355103 17.79729936786193 33.85101342458542 19.288658052197555 33 27.215366531562168 17.91752990710419 36.05206568358815 18.815037877745496 34 22.31638307146756 20.206403374082555 40.830247362034505 16.646966192415384 35 21.029751339124424 22.95832613373965 40.350057334946044 15.66186519218988 36 20.93192187782219 25.1584353575888 37.22072577921925 16.68891698536976 37 23.009218474704255 23.00764535615404 36.83771709035993 17.145419078781778 38 23.92854893563421 22.833086660788513 36.22110782214384 17.01725658143343 39 24.15976466331595 20.58094811109276 36.17278998245987 19.086497243131426 40 17.955822943924865 15.44141480421532 33.300788198369844 33.30197405348997 41 14.266539969117472 15.891436037534149 31.877103377281546 37.964920616066834 42 19.37329775234532 20.06028855185641 38.226636221078984 22.339777474719288 43 16.996415968023037 16.53926501772345 38.57693079395554 27.887388220297975 44 18.339245061175532 16.672256175749947 36.71952369491539 28.26897506815913 45 21.281876792239824 19.752892168624935 37.98427352579055 20.980957513344688 46 19.614775082286968 21.947123986897004 37.470555618912044 20.967545311903983 47 18.9578391794034 20.715979956331193 38.66574511867548 21.660435745589922 48 20.424988205692717 20.923101116527757 39.53884258531215 19.11306809246737 49 18.297200796954215 17.19053912769021 34.37437121496065 30.13788886039493 50 20.484338211305417 20.18403436985327 38.062725768349395 21.268901650491927 51 19.07739067784265 21.59238101237416 39.311487930959814 20.01874037882337 52 18.403077987203872 20.45100844167073 40.06256582773962 21.083347743385787 53 18.67727842899779 20.916286879347563 40.570723018060704 19.835711673593934 54 17.18962091554699 19.936655322329983 38.15002065587315 24.72370310624988 55 19.16473226580635 21.430538615887006 37.87661801156706 21.528111106739583 56 19.877066103514586 22.129674417872998 35.95010283817636 22.04315664043605 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 4.0 4 7.0 5 6.0 6 11.5 7 17.0 8 66.5 9 116.0 10 242.0 11 368.0 12 545.0 13 896.5 14 1071.0 15 1352.0 16 1633.0 17 1908.5 18 2184.0 19 2401.0 20 2618.0 21 3069.5 22 4307.5 23 5094.0 24 6045.0 25 6996.0 26 7802.0 27 8608.0 28 9232.5 29 9758.5 30 9660.0 31 8715.5 32 7771.0 33 6853.0 34 5935.0 35 5281.0 36 4627.0 37 4433.0 38 4293.5 39 4348.0 40 4340.0 41 4332.0 42 4582.5 43 4833.0 44 5605.5 45 6378.0 46 6653.5 47 6940.0 48 6951.0 49 7343.0 50 7735.0 51 8520.0 52 9305.0 53 10013.5 54 11789.5 55 12857.0 56 14040.0 57 15223.0 58 16924.0 59 18625.0 60 18740.0 61 18855.0 62 16643.0 63 12775.0 64 11119.0 65 9863.0 66 8607.0 67 7550.0 68 6493.0 69 5570.5 70 4648.0 71 3838.5 72 2504.0 73 1979.0 74 1607.0 75 1235.0 76 912.5 77 590.0 78 441.5 79 217.0 80 141.0 81 88.0 82 35.0 83 25.0 84 15.0 85 10.5 86 6.0 87 5.0 88 2.0 89 0.0 90 1.5 91 3.0 92 2.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.01844779470352039 14 7.850125405753358E-4 15 0.22961616811828567 16 0.0671185722191912 17 3.925062702876679E-4 18 0.003140050162301343 19 0.10244413654508132 20 0.09380899859875261 21 0.0 22 0.0 23 0.0 24 0.0 25 0.005102581513739682 26 0.008635137946328692 27 0.10519168043709498 28 0.12599451276234136 29 0.040820652109917456 30 0.017662782162945055 31 0.024727895028123073 32 0.09420150486904029 33 0.15778752065564247 34 0.049455790056246146 35 0.05063330886710915 36 0.037288095677328444 37 0.19664564141412158 38 0.2845670459585592 39 0.19586062887354624 40 0.7029787300852132 41 0.8646913134437323 42 0.1295270691949304 43 0.231578699469724 44 0.5188932893202969 45 0.21666346119879268 46 0.18840300973808058 47 0.05063330886710915 48 0.16210508962880682 49 0.5133982015362695 50 0.14287228238471109 51 0.30458486574323024 52 0.12599451276234136 53 0.18212290941347786 54 0.23864381233490206 55 0.23668128098346372 56 0.19232807244095723 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 254773.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.22028156177407 #Duplication Level Percentage of deduplicated Percentage of total 1 85.40282879054371 59.97010683843964 2 8.294097019134426 11.64827655968581 3 2.7797760352050354 5.855899676123087 4 1.1995788917445256 3.369390701354459 5 0.6870514059648865 2.4122471587133534 6 0.3905201254166608 1.6453459897378349 7 0.2824198758714997 1.3882122241636594 8 0.19181163054090292 1.0775253362724149 9 0.13070338808302007 0.8260225841040745 >10 0.5980754020999524 7.189842786805423 >50 0.02481879844663343 1.2369609922758473 >100 0.015364018086011172 1.6129041855002408 >500 0.0023636950901555647 1.3406980764989138 >1k 5.909237725388912E-4 0.42656689032525025 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1028 0.40349644585572253 No Hit CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 975 0.38269361353047615 No Hit CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 951 0.3732734630435721 No Hit CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 682 0.26768927633618944 No Hit CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 623 0.24453140638921708 No Hit CGTATTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 293 0.11500433719428668 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0011775188108630035 0.0 0.0 0.0 0.0 9 0.002355037621726007 0.0 0.0 0.0 0.0 10 0.012952706919493039 0.0 0.0 0.0 0.0 11 0.019232807244095724 0.0 0.0 0.0 0.0 12 0.022372857406397068 3.9250627028766783E-4 0.0 0.0 0.0 13 0.026297920109273745 3.9250627028766783E-4 0.0 0.0 0.0 14 0.028260451460712085 3.9250627028766783E-4 0.0 0.0 0.0 15 0.03336303297445177 3.9250627028766783E-4 0.0 0.0 0.0 16 0.03611057686646544 3.9250627028766783E-4 0.0 0.0 0.0 17 0.040820652109917456 3.9250627028766783E-4 0.0 0.0 0.0 18 0.04553072735336947 3.9250627028766783E-4 0.0 0.0 0.0 19 0.051418321407684486 3.9250627028766783E-4 0.0 0.0 0.0 20 0.05809092800257484 3.9250627028766783E-4 0.0 0.0 0.0 21 0.06515604086775287 3.9250627028766783E-4 0.0 0.0 0.0 22 0.07222115373293088 3.9250627028766783E-4 0.0 0.0 0.0 23 0.0792862665981089 3.9250627028766783E-4 0.0 0.0 0.0 24 0.08635137946328693 3.9250627028766783E-4 0.0 0.0 0.0 25 0.09537902367990328 3.9250627028766783E-4 0.0 0.0 0.0 26 0.1000890989233553 3.9250627028766783E-4 0.0 0.0 0.0 27 0.10440666789651964 3.9250627028766783E-4 0.0 0.0 0.0 28 0.10833173059939633 3.9250627028766783E-4 0.0 0.0 0.0 29 0.11186428703198534 3.9250627028766783E-4 0.0 0.0 0.0 30 0.11343431211313601 3.9250627028766783E-4 0.0 0.0 0.0 31 0.11539684346457435 3.9250627028766783E-4 0.0 0.0 0.0 32 0.12010691870802637 3.9250627028766783E-4 0.0 0.0 0.0 33 0.12246195632975236 3.9250627028766783E-4 0.0 0.0 0.0 34 0.12481699395147837 3.9250627028766783E-4 0.0 0.0 0.0 35 0.12638701903262906 3.9250627028766783E-4 0.0 0.0 0.0 36 0.13109709427608107 3.9250627028766783E-4 0.0 0.0 0.0 37 0.13384463816809475 3.9250627028766783E-4 0.0 0.0 0.0 38 0.13502215697895775 3.9250627028766783E-4 0.0 0.0 0.0 39 0.13580716951953306 3.9250627028766783E-4 0.0 0.0 0.0 40 0.1377697008709714 3.9250627028766783E-4 0.0 0.0 0.0 41 0.1401247384926974 3.9250627028766783E-4 0.0 0.0 0.0 42 0.14365729492528642 3.9250627028766783E-4 0.0 0.0 0.0 43 0.1464048388173001 3.9250627028766783E-4 0.0 0.0 0.0 44 0.15111491406075211 3.9250627028766783E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATTGT 30 1.009581E-6 50.247868 44 GTAAGCC 15 0.008260387 50.247868 35 AACGATT 50 7.2759576E-12 50.16835 47 ACGAAAC 15 0.008312274 50.168346 48 CAACGAT 50 7.2759576E-12 50.138596 46 AAGCTAT 25 2.0998508E-5 49.931286 10 ATCATCG 15 0.008469389 49.931282 10 ACGAATC 15 0.008469389 49.931282 10 GTAGCGA 15 0.00848258 49.91163 29 ACTAGAT 20 4.2471607E-4 49.833168 18 TCTGTTA 15 0.008542133 49.82338 21 GGATACG 20 4.2677944E-4 49.78426 8 TAGATAC 20 4.2677944E-4 49.78426 20 GGGTAAT 15 0.0085687 49.784256 8 CGTATTA 85 0.0 49.774483 1 GTATTAG 85 0.0 49.774483 2 CGTATTG 25865 0.0 48.78342 1 GTATTGG 25725 0.0 48.77803 2 TATTGGG 24985 0.0 48.768433 3 ATTGGGA 8855 0.0 48.73459 4 >>END_MODULE