FastQCFastQC Report
Fri 17 Jun 2016
SRR1526223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32828
Sequences flagged as poor quality0
Sequence length56
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTC2500.7615450225417326No Hit
GAACGCGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTC2090.6366516388448885No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTC2050.6244669184842208No Hit
GAACGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTC2010.6122821981235531No Hit
GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC1260.38381869136103325No Hit
GAACGCGGGGATGAGATGGACTCAAATGAATTGACGGGTCCGCACAGTTCTGAGTC1140.3472645302790301No Hit
GAACGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC960.29243328865602536No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGACGGGTCCGCACAGTTCTGAGTC740.22541732667235287No Hit
GAACGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.16449372486901426No Hit
GAACGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTC520.15840136468868038No Hit
GAACGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTC490.14926282441817962No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC380.11575484342634336No Hit
GAACGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.11575484342634336No Hit
GAACGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTC360.10966248324600951No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG330.10052394297550872No Hit
GAACGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA330.10052394297550872No Hit
GAACGCGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG330.10052394297550872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCC150.00822841550.1981644
TGCACAG150.00822841550.1981641
TTCGTGG150.00822841550.1981615
AATTCGT150.00822841550.1981613
CCAGTCC204.044097E-450.1981644
TGTTCAC150.00822841550.1981615
CCGCACA600.050.1981640
TGGGGAT150.00822841550.1981615
ATTCGTG150.00822841550.1981614
TCCGCAC600.050.1981639
CGATGGA309.891864E-750.1981614
GGTATCG150.00822841550.1981615
TTTGTCA150.00822841550.1981641
CGTTCGC150.00827860150.12116646
CAGTCCT309.99762E-750.12116645
TGCAATC204.0749347E-450.12116650
GATGCGA309.99762E-750.12116610
ATGCGAT309.99762E-750.12116611
GAATGTA150.00827860150.1211669
GTCCTGA309.99762E-750.12116647
Produced by FastQC (version 0.11.5)