##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32828
Sequences flagged as poor quality	0
Sequence length	56
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.48047398562203	39.0	38.0	39.0	33.0	40.0
2	36.090928475691484	38.0	36.0	39.0	29.0	40.0
3	35.0310405751188	39.0	35.0	39.0	21.0	40.0
4	37.04593639575972	39.0	37.0	39.0	33.0	40.0
5	36.77765931521871	38.0	36.0	39.0	32.0	40.0
6	37.09214694772755	39.0	38.0	39.0	33.0	40.0
7	37.23796758864384	39.0	37.0	39.0	33.0	40.0
8	37.25944315827952	39.0	38.0	39.0	33.0	40.0
9	37.30678079688071	39.0	38.0	39.0	33.0	40.0
10	36.45500792006823	39.0	35.0	39.0	31.0	40.0
11	35.74384671621786	38.0	35.0	39.0	29.0	40.0
12	34.37797002558791	38.0	33.0	39.0	23.0	40.0
13	34.19038625563543	38.0	33.0	39.0	22.0	40.0
14	34.235165102960885	38.0	33.0	39.0	23.0	40.0
15	34.16842329718533	38.0	33.0	39.0	23.0	40.0
16	33.65340562934081	38.0	32.0	39.0	22.0	40.0
17	33.59580236383575	37.0	31.0	39.0	22.0	40.0
18	33.267028146704035	37.0	31.0	39.0	19.0	40.0
19	34.14713049835506	38.0	32.0	39.0	24.0	40.0
20	34.184263433654195	38.0	32.0	39.0	24.0	40.0
21	34.933654197636166	38.0	33.0	39.0	26.0	40.0
22	34.629158035823075	38.0	33.0	39.0	25.0	40.0
23	35.13774826367735	38.0	34.0	39.0	27.0	40.0
24	34.59455342999878	38.0	33.0	39.0	25.0	40.0
25	34.885189472401606	38.0	33.0	39.0	26.0	40.0
26	35.325575728037045	38.0	35.0	39.0	28.0	40.0
27	35.267028146704035	38.0	35.0	39.0	28.0	40.0
28	35.32259047154868	38.0	35.0	39.0	28.0	40.0
29	35.00594005117583	38.0	35.0	39.0	27.0	40.0
30	35.00042646521263	38.0	35.0	39.0	27.0	40.0
31	35.16485926647984	38.0	35.0	39.0	28.0	40.0
32	35.02839039844036	38.0	35.0	39.0	27.0	40.0
33	35.10548921652248	38.0	35.0	39.0	28.0	40.0
34	35.065736566345805	38.0	35.0	39.0	28.0	40.0
35	34.96566955038382	38.0	35.0	39.0	27.0	40.0
36	34.53210673815036	38.0	34.0	39.0	28.0	40.0
37	34.24936030218107	37.0	33.0	39.0	27.0	40.0
38	33.9050810283904	37.0	33.0	39.0	26.0	40.0
39	32.600463019373706	36.0	31.0	38.0	18.0	39.0
40	30.349884245156574	35.0	27.0	38.0	10.0	39.0
41	27.071920311928842	32.0	16.0	38.0	6.0	39.0
42	31.089253076641892	35.0	29.0	38.0	12.0	39.0
43	29.59430973559157	34.0	27.0	38.0	6.0	39.0
44	29.556110637260876	34.0	27.0	38.0	5.0	39.0
45	30.59778238089436	35.0	29.0	38.0	4.0	39.0
46	30.277263311806994	35.0	28.0	38.0	4.0	40.0
47	29.88266114292677	35.0	27.0	38.0	4.0	40.0
48	29.852625807237725	35.0	27.0	38.0	4.0	39.0
49	27.287468015109052	34.0	16.0	38.0	4.0	39.0
50	29.499573534787377	35.0	27.0	38.0	4.0	40.0
51	29.681369562568538	35.0	27.0	39.0	4.0	40.0
52	29.3449189716096	35.0	27.0	39.0	4.0	40.0
53	28.955586694285365	35.0	26.0	39.0	4.0	40.0
54	27.944254904349947	35.0	18.0	39.0	4.0	40.0
55	28.17009869623492	35.0	23.0	38.0	4.0	40.0
56	26.71454246375046	33.0	17.0	38.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.0206150929877111
1	2	2.813278542308879
1	3	4.133811723986199
1	4	1.2254886410103438
1	5	1.567637314515835
1	6	1.069201311771259
1	7	1.3225567243949286
1	8	1.1840770955066233
1	9	1.2990761222327976
1	10	0.9281696951503733
1	11	-4.601860821465937
1	12	-13.212491416267323
1	13	-10.476166980492206
1	14	-6.2460963267190515
1	15	-7.203771242041139
1	16	-23.46149458203889
1	17	-5.375660041525855
1	18	-10.202860758874511
1	19	-5.762087746706612
1	20	-6.043679554856624
1	21	-7.394384218142381
1	22	-2.020389840101547
1	23	-3.1902774818098685
1	24	-3.8515272716584192
1	25	-3.0021150331407753
1	26	2.5278360833609526
1	27	2.327041541758696
1	28	1.7217537788432935
1	29	2.5416538518521676
1	30	2.8695635360092453
1	31	1.8561507261610402
1	32	2.671428316449436
1	33	1.8188022137839326
1	34	1.1971702436288183
1	35	2.0259765939273393
1	36	1.5811397532753375
1	37	2.2983049238838404
1	38	1.901997451676003
1	39	2.948075530467559
1	40	5.0959529611009415
1	41	-9.071164580202815
1	42	-8.047855589671403
1	43	-16.61879558443997
1	44	-22.78435783976924
1	45	-11.649797263106851
1	46	-16.831861308823935
1	47	-23.344563880575173
1	48	-13.41375978720351
1	49	-20.02821182122689
1	50	-15.433216251151501
1	51	-10.935898968730285
1	52	-11.06639450455824
1	53	-11.50007325264713
1	54	-10.480484498919402
1	55	-11.127891389571365
1	56	-5.1988588158935585
2	1	1.5016677245666585
2	2	3.4532785423088797
2	3	4.986443302933566
2	4	1.928646535747184
2	5	1.9318478408316224
2	6	2.064990785455471
2	7	2.0362409349212456
2	8	2.023024463927676
2	9	1.8601287538117433
2	10	2.0123802214661595
2	11	0.6991918101130139
2	12	-4.6072282583725865
2	13	-1.8540617173343108
2	14	1.7149563048598964
2	15	2.1951761263799128
2	16	2.382715944276896
2	17	3.110655747947831
2	18	-1.4281239167692483
2	19	2.757912253293391
2	20	2.3868467609328476
2	21	2.9098263081734075
2	22	2.463820686214241
2	23	1.9276172550322386
2	24	0.8547885178152654
2	25	2.3473586510697544
2	26	2.5520466096767436
2	27	2.4080941733376378
2	28	2.850174831474874
2	29	3.079548588694273
2	30	2.5464056412724005
2	31	1.6445717787926242
2	32	1.6082704217125965
2	33	2.1714337927312997
2	34	1.9150649804709232
2	35	2.0596608044536566
2	36	2.2485081743279665
2	37	2.843568081778578
2	38	3.1230500832549453
2	39	2.730180793625454
2	40	6.928584540048313
2	41	-6.654322474939658
2	42	-5.695224010724033
2	43	-11.915637689703125
2	44	-13.392778892400818
2	45	-5.238218315738429
2	46	-11.291861308823936
2	47	-17.997195459522544
2	48	-7.867443997729826
2	49	-12.47242234754268
2	50	-7.134268882730449
2	51	-1.976951600309235
2	52	-1.6463945045582378
2	53	-2.1369153579102864
2	54	-0.92995818312993
2	55	-0.8436808632555746
2	56	2.356930657790649
3	1	1.19698687350283
3	2	2.85583173379824
3	3	4.803464579529312
3	4	1.403540152768464
3	5	1.070996777001838
3	6	1.4913737641788742
3	7	1.2536877434318825
3	8	1.4757904213744837
3	9	0.5605542857266386
3	10	0.7294014980619039
3	11	1.5638726611768377
3	12	3.0097930182231565
3	13	1.4084914741550492
3	14	1.5260201346471334
3	15	1.513473998720336
3	16	3.1163329655534966
3	17	3.1506557479478303
3	18	3.479110125783947
3	19	2.9294016149955127
3	20	2.177485058805189
3	21	2.640890137960646
3	22	2.4616930266397787
3	23	1.6165534252450016
3	24	3.6215970284535643
3	25	1.5107629063888979
3	26	1.1482168224426985
3	27	0.7374558754653009
3	28	0.4306003633897717
3	29	1.2740166738006522
3	30	1.8191715987192083
3	31	1.1820185873032614
3	32	2.4082704217125936
3	33	1.4139869842206636
3	34	0.6529373208964557
3	35	1.4783842087089738
3	36	1.4753166849662662
3	37	1.658887230714754
3	38	1.589007530063462
3	39	1.7157127085190744
3	40	5.425180284729166
3	41	5.899720078251828
3	42	1.8771164148078796
3	43	-2.9301057748095083
3	44	0.30466791610982114
3	45	2.715824237453056
3	46	0.7106918826654258
3	47	-3.5354933318629698
3	48	-0.16403974241067587
3	49	3.000343609904128
3	50	1.9648800534397637
3	51	2.0473037188397036
3	52	2.337860814590698
3	53	1.8801059186854552
3	54	1.8896162849551779
3	55	2.2478084984465525
3	56	0.5318242748119282
4	1	1.5041677245666563
4	2	3.313278542308879
4	3	4.373943302933569
4	4	1.9886465357471863
4	5	1.6476811741649584
4	6	0.8849907854554715
4	7	1.9212409349212436
4	8	1.6380244639276782
4	9	1.7409620871450784
4	10	2.1332135547994966
4	11	1.8191918101130113
4	12	3.601105074960749
4	13	3.428438282665688
4	14	2.2791229715265615
4	15	4.5001761263799125
4	16	3.8268826109435636
4	17	4.254822414614495
4	18	4.816876083230753
4	19	3.6962455866267234
4	20	3.8126800942661845
4	21	2.9356596415067457
4	22	2.851320686214244
4	23	2.384283921698902
4	24	3.34145518448193
4	25	3.412358651069752
4	26	2.46204660967674
4	27	1.8105941733376412
4	28	2.215174831474876
4	29	2.282881922027606
4	30	2.648072307939067
4	31	0.12040511212595817
4	32	0.32493708837925794
4	33	1.0797671260646382
4	34	-0.20743501952907906
4	35	0.33299413778699005
4	36	0.4660081743279676
4	37	1.3202347484452446
4	38	0.3855500832549481
4	39	2.248514126958792
4	40	4.752751206714976
4	41	7.874010858393678
4	42	3.713109322609302
4	43	6.227695643630209
4	44	4.0763877742658465
4	45	4.148448350928234
4	46	4.168138691176065
4	47	4.602804540477457
4	48	4.050056002270175
4	49	6.912577652457319
4	50	4.437397783936216
4	51	4.018048399690766
4	52	4.5586054954417605
4	53	4.8047513087563765
4	54	4.875875150203402
4	55	4.827152470077756
4	56	5.61693065779065
5	1	1.755621212938749
5	2	3.348162263239111
5	3	4.907373535491708
5	4	2.139809326444862
5	5	1.775103654785113
5	6	1.733827994757796
5	7	1.7613572139910119
5	8	1.7281407429974465
5	9	1.5224543352070938
5	10	2.298426733094068
5	11	3.214540647322316
5	12	4.509050811394857
5	13	4.179891771037781
5	14	4.51216560718548
5	15	4.898431940333403
5	16	4.734343851253641
5	17	4.220423189808294
5	18	4.761643525091216
5	19	4.156516904456176
5	20	3.6435909469793586
5	21	3.4409890988710856
5	22	3.6987044071444757
5	23	3.328082371311311
5	24	4.081765262001312
5	25	3.8571260929302156
5	26	2.9562326561883694
5	27	2.387629057058568
5	28	2.3459887849632466
5	29	3.0018741700896214
5	30	3.2575684319700784
5	31	2.875734569490298
5	32	2.3850146077591035
5	33	2.55748030435921
5	34	0.8666928874476625
5	35	2.615939874221098
5	36	3.1447872440954114
5	37	2.8919401748018387
5	38	3.8463058972084383
5	39	4.104134281997549
5	40	6.916026400513431
5	41	9.502886827385925
5	42	6.307101570671318
5	43	7.295525100994549
5	44	7.277453665738719
5	45	6.063177033098782
5	46	6.551859621408621
5	47	7.1260603544309475
5	48	4.973021118549248
5	49	9.392228815248014
5	50	7.041079954478857
5	51	6.898862353179137
5	52	7.282442704744085
5	53	7.844479990926924
5	54	8.788181351753792
5	55	7.195854020465351
5	56	6.709953913604604
6	1	1.4643949972939296
6	2	2.745096724127059
6	3	4.663716030206295
6	4	1.965919263019913
6	5	1.7291205681043493
6	6	1.6577180581827449
6	7	1.637150025830337
6	8	1.4902971912004048
6	9	1.2883105719935628
6	10	1.8396529487388875
6	11	0.9101009010221048
6	12	4.279135377991047
6	13	3.911392828120235
6	14	4.743138123041717
6	15	3.716085217289006
6	16	2.7227159442768993
6	17	2.991564838856924
6	18	4.964603355958026
6	19	4.374275889657021
6	20	2.916846760932849
6	21	2.0170990354461367
6	22	3.2547297771233374
6	23	2.8994354368504176
6	24	3.0838794269061722
6	25	3.219176832887932
6	26	2.8711375187676467
6	27	1.9526396278830944
6	28	2.0219930132930557
6	29	2.108639497785177
6	30	1.3564056412724028
6	31	1.065480869701716
6	32	1.3173613308035002
6	33	0.32597924727675576
6	34	1.241428616834554
6	35	1.4314789862718342
6	36	1.6421445379643345
6	37	1.7217498999604004
6	38	1.9594137196185812
6	39	1.6538171572618232
6	40	5.194039085502858
6	41	4.642950252333069
6	42	3.8911396256395996
6	43	5.7579986739332405
6	44	5.097221107599182
6	45	3.1635998660797497
6	46	3.304502327539698
6	47	4.625531813204731
6	48	4.203465093179268
6	49	7.151214016093686
6	50	4.213912935451372
6	51	4.188502945145313
6	52	4.479060040896307
6	53	4.172175551180622
6	54	6.752769089597344
6	55	4.374500954926241
6	56	3.3896579305179237
7	1	1.1689404518393829
7	2	2.9041876332179726
7	3	4.663716030206295
7	4	1.965919263019913
7	5	1.1154842044679896
7	6	2.044081694546378
7	7	0.6144227531030637
7	8	0.8993881002913113
7	9	0.8564923901753758
7	10	0.6123802214661609
7	11	2.228282719203918
7	12	3.233680832536507
7	13	2.661392828120235
7	14	2.470410850314444
7	15	3.602448853652639
7	16	3.1090795806405325
7	17	2.9688375661296504
7	18	4.214603355958026
7	19	2.760639526020661
7	20	2.4623013063873955
7	21	1.2670990354461367
7	22	2.0047297771233374
7	23	1.6267081641231442
7	24	2.3338794269061722
7	25	1.5600859237970255
7	26	1.3029557005858337
7	27	0.5435487187921879
7	28	0.6810839223839693
7	29	1.7677304068760904
7	30	1.174587459454223
7	31	-0.07088276666192428
7	32	0.2946340580762268
7	33	0.8487065200040291
7	34	0.9005195259254677
7	35	0.18147898627183423
7	36	1.2557809016006942
7	37	1.380840809051307
7	38	2.4139591741640416
7	39	1.3129080663527297
7	40	3.625857267321038
7	41	0.14295025233306902
7	42	3.34568508018506
7	43	4.68981685575142
7	44	4.142675653053729
7	45	4.004508956988843
7	46	5.236320509357885
7	47	5.625531813204731
7	48	5.408010547724722
7	49	6.605759470639139
7	50	5.054822026360458
7	51	5.506684763327133
7	52	5.456332768169034
7	53	4.058539187544259
7	54	4.411859998688254
7	55	3.1245009549262406
7	56	3.3896579305179237
8	1	2.075001057899989
8	2	3.6577229867533205
8	3	5.230887747378013
8	4	2.0886465357471877
8	5	2.5296256186094013
8	6	2.229435229899913
8	7	2.2295742682545736
8	8	2.263024463927678
8	9	2.39790653158952
8	10	2.156824665910605
8	11	3.019191810113014
8	12	4.614993963849635
8	13	4.943716060443464
8	14	4.608289638193227
8	15	5.275176126379911
8	16	5.333827055388014
8	17	4.817322414614495
8	18	6.314098305452973
8	19	3.8712455866267206
8	20	4.755735649821737
8	21	3.7009374192845215
8	22	4.408265130658691
8	23	3.956506143921125
8	24	4.263677406704154
8	25	4.187358651069751
8	26	2.723157720787853
8	27	2.759205284448754
8	28	1.9346192759193173
8	29	3.2328819220276017
8	30	2.6286278634946285
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115	7	0.8479650728522756
115	8	0.883714119100091
115	9	1.0132322020876074
115	10	0.5261733249144385
115	11	-3.111842672645608
115	12	-13.124469637682932
115	13	-8.116820338023967
115	14	-7.842285074450448
115	15	-7.1248238736200875
115	16	-8.087628883309307
115	17	-2.3321028727418245
115	18	-1.8943308133209698
115	19	-3.1931222294652315
115	20	-2.815911859756806
115	21	1.513964239207894
115	22	2.1293379275935536
115	23	1.950375875721896
115	24	1.4851333454014721
115	25	0.5459793407249265
115	26	0.7292879889870818
115	27	0.6274045181652284
115	28	0.5729334521645342
115	29	0.9754106576597863
115	30	0.9167504688586092
115	31	0.5004338477581385
115	32	1.1324083527470776
115	33	-0.007876552096284684
115	34	0.07920291150540493
115	35	0.016902183763995993
115	36	2.3043702432934836
115	37	1.300119805916509
115	38	1.167877669461845
115	39	1.9108704487978692
115	40	5.769274195220724
115	41	5.631194766439652
115	42	0.8668449547932084
115	43	-6.321154931082436
115	44	-3.8596754441249566
115	45	0.5852299601236375
115	46	-4.366344067444626
115	47	-4.104092011246681
115	48	-3.646064687384996
115	49	-16.31587062340475
115	50	-3.112889572385619
115	51	-3.3914343589299243
115	52	-2.2043255390409975
115	53	-2.7713981165309782
115	54	-7.459613355543723
115	55	-2.7712670701521276
115	56	-0.831345204278314
116	1	0.9929497758487074
116	2	2.659432388462726
116	3	4.109520226010488
116	4	1.360441407542055
116	5	0.6492837382675205
116	6	-0.2047528042881197
116	7	0.988548627228937
116	8	1.0578962587994738
116	9	1.1944877281707136
116	10	1.214944324030263
116	11	-2.4885004975792953
116	12	-14.53030518144951
116	13	-6.890471973744567
116	14	-4.607094977191384
116	15	-7.8427725915688065
116	16	-4.315745594184641
116	17	-2.1313955341034507
116	18	-8.872226480871813
116	19	-3.441574926193791
116	20	-3.4869993929133045
116	21	1.8923904107375122
116	22	1.4202309426244994
116	23	1.6983864858014712
116	24	1.1491474921742366
116	25	1.4283842920953944
116	26	1.7505081481382803
116	27	2.19681212205559
116	28	2.115815857115905
116	29	2.058522947668628
116	30	2.196149231015994
116	31	1.2245717787926225
116	32	1.1518601653023381
116	33	1.2688696901672003
116	34	1.2268598522657896
116	35	1.807353112145961
116	36	1.233636379456172
116	37	1.762542440752938
116	38	2.310229570434437
116	39	4.546591050035712
116	40	6.353712745176516
116	41	5.434908294291112
116	42	-0.3798393953394168
116	43	-7.432560766626203
116	44	-5.575855815477741
116	45	0.9689611714410553
116	46	-2.6651946421572674
116	47	-2.768990331317415
116	48	-3.3746234849093106
116	49	-5.776524911645247
116	50	-1.9440124724740393
116	51	0.104586861229226
116	52	0.9592465210827861
116	53	-0.7625563835513134
116	54	-4.462265875437623
116	55	0.10760118802647511
116	56	-1.0497360088760175
117	1	0.9416677245666563
117	2	2.9732785423088757
117	3	4.266443302933567
117	4	1.648646535747183
117	5	1.9518478408316255
117	6	1.504990785455469
117	7	1.5362409349212456
117	8	1.3830244639276756
117	9	1.5001287538117438
117	10	1.4723802214661603
117	11	-4.30080818988699
117	12	-10.207228258372588
117	13	-11.514061717334311
117	14	-12.645043695140103
117	15	-8.524823873620086
117	16	-11.737284055723102
117	17	-10.36934425205217
117	18	-12.228123916769249
117	19	-4.662087746706611
117	20	-5.173153239067151
117	21	1.8298263081734092
117	22	1.0438206862142465
117	23	-0.212382744967762
117	24	1.9347885178152637
117	25	0.987358651069755
117	26	2.0920466096767427
117	27	2.1280941733376437
117	28	1.890174831474873
117	29	2.339548588694271
117	30	1.4864056412724054
117	31	0.5845717787926219
117	32	1.0882704217125934
117	33	1.4914337927313
117	34	-0.7249350195290774
117	35	0.2996608044536515
117	36	0.06850817432796674
117	37	1.323568081778582
117	38	1.1430500832549484
117	39	2.690180793625455
117	40	5.1485845400483115
117	41	1.6256775250603432
117	42	-3.1552240107240337
117	43	-7.5156376897031265
117	44	-8.732778892400816
117	45	-3.638218315738431
117	46	-2.6918613088239347
117	47	-4.177195459522544
117	48	-3.007443997729826
117	49	-8.132422347542683
117	50	-3.8342688827304485
117	51	-3.0369516003092336
117	52	-2.3863945045582398
117	53	-2.6569153579102895
117	54	-10.72995818312993
117	55	-3.4236808632555764
117	56	-1.86306934220935
118	1	0.5623573797390691
118	2	2.158106128515776
118	3	3.8071329581059814
118	4	0.9369223978161543
118	5	1.2690892201419643
118	6	0.9367149233865035
118	7	0.778999555610902
118	8	0.9181968777207814
118	9	0.8408184089841555
118	10	0.7675526352592641
118	11	-2.8704633623007823
118	12	-10.710676534234654
118	13	-14.185785855265348
118	14	-9.600905764105619
118	15	-4.4006859425856035
118	16	-14.604870262619652
118	17	-10.435551148603892
118	18	-11.96329633056235
118	19	-5.67588085015489
118	20	-6.022808411480945
118	21	0.16913665300099723
118	22	1.4741655138004504
118	23	0.9848586343425794
118	24	1.3472023109187106
118	25	1.23563451313872
118	26	1.4879086786422562
118	27	1.2825769319583316
118	28	0.33155414181970144
118	29	1.492652036970135
118	30	1.6064056412724028
118	31	-0.1892213246556551
118	32	-4.902074405873613
118	33	0.3024682754899217
118	34	1.079202911505405
118	35	-0.3624081610635841
118	36	1.9250598984658964
118	37	1.3346025645371995
118	38	2.5127052556687417
118	39	4.152249759142695
118	40	6.355481091772447
118	41	5.769125800922414
118	42	0.7633966789311373
118	43	-8.734948034530714
118	44	-8.963123719987024
118	45	-1.1733907295315333
118	46	-10.400826826065316
118	47	-12.00064373538461
118	48	-9.680547446005686
118	49	-13.41931889926682
118	50	-10.181855089627
118	51	-8.63281366927475
118	52	-8.031911745937549
118	53	-8.3576050130827
118	54	-12.80444094175062
118	55	-8.150577414979715
118	56	-5.38306934220935
119	1	0.40595343885237156
119	2	2.3847071137374485
119	3	4.007871874362138
119	4	0.2386465357471863
119	5	0.5018478408316227
119	6	0.1707050711697562
119	7	0.04624093492124359
119	8	-0.0584041075008912
119	9	-0.20844267475968792
119	10	-0.3519054928195544
119	11	-7.680808189886989
119	12	-13.17865682980116
119	13	-13.419776003048597
119	14	-10.575043695140103
119	15	-13.410538159334372
119	16	-17.670141198580243
119	17	-17.09934425205217
119	18	-18.20098105962639
119	19	-7.583516318135185
119	20	-9.940296096210009
119	21	-1.6744594061123053
119	22	-0.37189359950004075
119	23	-0.3895256021106164
119	24	-0.03949719647044958
119	25	-0.319784206073102
119	26	1.3906180382481708
119	27	0.8552370304804953
119	28	1.0187462600463064
119	29	0.23526287440855498
119	30	1.2492627841295487
119	31	-0.8825710783502316
119	32	-4.484586721144549
119	33	-0.5571376358401281
119	34	-1.5020778766719332
119	35	-0.32176776697491505
119	36	-0.2929203971006018
119	37	1.0107109389214344
119	38	1.1444786546835175
119	39	2.3973236507683104
119	40	5.434298825762596
119	41	2.6656775250603424
119	42	-1.3166525821526065
119	43	-12.891351975417413
119	44	-10.331350320972245
119	45	-6.125361172881288
119	46	-17.081861308823935
119	47	-16.0043383166654
119	48	-11.85172971201554
119	49	-14.533850918971252
119	50	-9.74712602558759
119	51	-8.32123731459495
119	52	-9.137823075986812
119	53	-9.915486786481715
119	54	-12.591386754558501
119	55	-9.47939514896986
119	56	-5.847355056495065
120	1	-0.4583322754333423
120	2	1.9132785423088805
120	3	3.28644330293357
120	4	0.6386465357471849
120	5	0.3518478408316241
120	6	0.2849907854554701
120	7	0.09624093492124075
120	8	0.3630244639276796
120	9	0.4701287538117427
120	10	-0.1376197785338391
120	11	-4.78080818988699
120	12	-14.357228258372587
120	13	-15.984061717334313
120	14	-15.575043695140103
120	15	-13.474823873620089
120	16	-15.9272840557231
120	17	-12.999344252052168
120	18	-17.708123916769246
120	19	-6.912087746706611
120	20	-15.48315323906715
120	21	-6.560173691826591
120	22	-4.236179313785755
120	23	-3.0323827449677623
120	24	-9.375211482184735
120	25	-3.212641348930248
120	26	1.7620466096767373
120	27	0.7980941733376383
120	28	1.140174831474873
120	29	1.4495485886942703
120	30	0.3564056412724028
120	31	-0.9754282212073804
120	32	-11.091729578287406
120	33	0.2714337927313011
120	34	-0.8949350195290791
120	35	-1.5003391955463456
120	36	-1.371491825672031
120	37	-0.3464319182214197
120	38	0.523050083254951
120	39	-0.9598192063745437
120	40	4.098584540048314
120	41	0.2656775250603438
120	42	-3.2452240107240335
120	43	-8.705637689703128
120	44	-9.052778892400816
120	45	-5.76821831573843
120	46	-15.731861308823936
120	47	-17.897195459522543
120	48	-12.987443997729825
120	49	-18.862422347542683
120	50	-12.354268882730448
120	51	-10.756951600309232
120	52	-10.06639450455824
120	53	-9.386915357910286
120	54	-13.86995818312993
120	55	-8.143680863255575
120	56	-7.38306934220935
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	180.0
5	13.0
6	13.0
7	43.0
8	56.0
9	57.0
10	26.0
11	29.0
12	39.0
13	41.0
14	47.0
15	66.0
16	99.0
17	140.0
18	136.0
19	189.0
20	230.0
21	309.0
22	397.0
23	461.0
24	562.0
25	675.0
26	656.0
27	798.0
28	938.0
29	1027.0
30	1266.0
31	1459.0
32	1630.0
33	1924.0
34	2253.0
35	2726.0
36	3472.0
37	4383.0
38	4112.0
39	2373.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	97.1822834165956	2.7080541001584013	0.05787742171317168	0.05178506153283782
8	99.39076398196661	0.5208967954185452	0.039600341172170095	0.04873888144267089
9	97.23102229803825	1.9008163762641646	0.5544047764103814	0.3137565492871939
10	49.777628853417816	36.526745461191666	6.920921164859266	6.7747045205312535
11	27.708054100158403	41.568173510417935	16.421956866089925	14.301815523333739
12	32.161569391982454	13.57682466187401	32.408309979285974	21.853295966857562
13	33.69588398379185	14.498979374219298	22.74624501112025	29.0588916308686
14	21.77171230998873	30.666829134553875	23.48067139854388	24.080787156913516
15	37.08441459319188	17.33475805220577	23.147611051747823	22.433216302854525
16	24.522205626847928	30.377663303563263	23.24199103849788	21.858140031090926
17	25.389911051541368	19.925063969781895	32.3230169367613	22.362008041915438
18	36.578530522724506	21.143536005848667	20.321067381503592	21.956866089923235
19	35.412474849094565	24.62654716175843	17.995853911346867	21.965124077800134
20	24.804830446450353	33.605757501829714	19.025981946816298	22.563430104903635
21	23.723650542220057	20.09869623492141	22.623979529669793	33.55367369318874
22	24.600950408188133	20.814548556110637	32.310832216400634	22.273668819300596
23	23.921652248080907	23.297185329596687	26.821615693919824	25.959546728402582
24	24.402948702327283	34.43706591933715	21.094797124405996	20.065188253929573
25	22.663579870841964	34.45229681978799	22.602656269038626	20.281467040331425
26	21.944977607165708	31.837431069676754	26.69774243670597	19.519848886451573
27	27.241126966703256	17.91681912428345	33.00402488108306	21.838029027930236
28	31.70768902308842	20.312928904748834	29.496446762436328	18.482935309726418
29	19.567867373681967	31.230572316694094	29.911013591759616	19.290546717864327
30	17.49847653869592	29.542961608775137	34.448507007922	18.510054844606945
31	18.283502483923076	19.05153759409954	46.085154368961625	16.579805553015756
32	19.225491690806525	17.310565635005336	47.66885195914011	15.795090715048026
33	28.449354533524584	18.427076021607107	39.00265511032441	14.120914334543903
34	16.798756325062488	28.17777235871487	40.76693287813205	14.256538438090594
35	17.03852718849061	18.818580833942942	41.34052670080468	22.802365276761766
36	27.43783520234032	18.899317406143346	40.49549000487567	13.167357386640663
37	27.85153984677838	19.503708451606997	39.69111497726093	12.953636724353693
38	26.085362500381905	18.91173505239681	42.17103052152394	12.831871925697353
39	17.05457096665955	19.586370984961718	50.114388555043774	13.24466949333496
40	12.18398553611375	21.36181166304048	36.126007415806086	30.328195385039685
41	12.283986617146015	13.990607446514625	33.54001043617054	40.185395500168816
42	23.829280942066568	18.237286067299184	41.017114616065165	16.916318374569084
43	13.93978258214242	17.332356174422866	40.378038353487234	28.34982288994748
44	13.90681880754994	24.34763987885833	39.007005414665485	22.738535898926244
45	15.441445841983148	19.90169739894981	41.632067407497864	23.02478935156918
46	15.548026737478251	27.723346457894575	41.77883588193999	14.949790922687178
47	23.82766080624029	18.193729242207258	42.176787836314325	15.801822115238123
48	15.424752837788356	18.531063102648602	51.83998535335043	14.20419870621262
49	12.760352854692478	16.138814016172507	45.610757167360944	25.49007596177407
50	14.263490223591496	19.235579416160814	43.501204892779796	22.999725467467893
51	16.498121047325164	19.742751519965783	49.80599431731386	13.953133115395191
52	23.66093216203026	21.22376769155686	40.26964372864812	14.845656417764763
53	15.358788877697402	30.00640966944419	40.16420962671306	14.470591826145347
54	22.053928604146943	20.682810639142517	39.389867774147255	17.87339298256329
55	15.23571079628725	19.84306301905227	49.12982413287738	15.791402051783097
56	16.890964020873387	21.239586194268973	38.695108181513014	23.174341603344626
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	5.5
13	23.0
14	35.0
15	74.5
16	114.0
17	123.5
18	133.0
19	204.5
20	276.0
21	315.0
22	361.0
23	368.0
24	469.5
25	571.0
26	752.0
27	933.0
28	1089.0
29	1387.0
30	1529.0
31	1647.5
32	1766.0
33	1766.5
34	1767.0
35	1581.0
36	1395.0
37	1172.0
38	828.5
39	708.0
40	599.0
41	490.0
42	476.5
43	463.0
44	482.0
45	501.0
46	557.0
47	704.5
48	796.0
49	1059.0
50	1322.0
51	1624.5
52	1927.0
53	1831.0
54	1544.5
55	1354.0
56	1254.0
57	1154.0
58	1185.0
59	1216.0
60	1322.5
61	1429.0
62	1492.0
63	1536.5
64	1518.0
65	1350.0
66	1182.0
67	1075.5
68	969.0
69	852.5
70	736.0
71	639.0
72	495.0
73	448.0
74	367.0
75	286.0
76	250.0
77	214.0
78	169.5
79	90.5
80	56.0
81	39.0
82	22.0
83	16.0
84	10.0
85	10.5
86	11.0
87	5.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.015230900450834655
14	0.0030461800901669307
15	0.22237114658218596
16	0.06396978189350555
17	0.0
18	0.0
19	0.07920068234434019
20	0.10966248324600951
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.015230900450834655
27	0.09747776288534178
28	0.12489338369684416
29	0.042646521262337034
30	0.024369440721335445
31	0.05178506153283782
32	0.10052394297550872
33	0.18581698550018277
34	0.06701596198367248
35	0.06092360180333862
36	0.03655416108200317
37	0.1980017058608505
38	0.29547946874619224
39	0.13707810405751186
40	0.5940051175825515
41	0.7584988424515657
42	0.14926282441817962
43	0.24369440721335447
44	0.42341903253320334
45	0.21932496649201902
46	0.1980017058608505
47	0.027415620811502377
48	0.17058608504934814
49	0.5483124162300475
50	0.13707810405751186
51	0.29547946874619224
52	0.13403192396734495
53	0.1980017058608505
54	0.24674058730352139
55	0.23150968685268672
56	0.1797246253198489
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	32828.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.43730961374436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.50655384104861	63.52808578043134
2	7.711133233781317	11.325697575240648
3	2.567612410817986	5.65675642743999
4	1.2278081964493113	3.606677226757646
5	0.6719761075161772	2.4674058730352137
6	0.3193960511033682	1.407335201657122
7	0.22814003650240586	1.1727793347142683
8	0.1866600298656048	1.096624832460095
9	0.11614401858304298	0.7676373827220665
>10	0.42309606769537084	4.764225661021079
>50	0.016592002654720425	0.8407457048860728
>100	0.024888003982080638	3.366028999634459
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTC	250	0.7615450225417326	No Hit
GAACGCGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTC	209	0.6366516388448885	No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTC	205	0.6244669184842208	No Hit
GAACGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTC	201	0.6122821981235531	No Hit
GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC	126	0.38381869136103325	No Hit
GAACGCGGGGATGAGATGGACTCAAATGAATTGACGGGTCCGCACAGTTCTGAGTC	114	0.3472645302790301	No Hit
GAACGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC	96	0.29243328865602536	No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGACGGGTCCGCACAGTTCTGAGTC	74	0.22541732667235287	No Hit
GAACGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54	0.16449372486901426	No Hit
GAACGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTC	52	0.15840136468868038	No Hit
GAACGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTC	49	0.14926282441817962	No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	38	0.11575484342634336	No Hit
GAACGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38	0.11575484342634336	No Hit
GAACGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTC	36	0.10966248324600951	No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	33	0.10052394297550872	No Hit
GAACGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	33	0.10052394297550872	No Hit
GAACGCGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG	33	0.10052394297550872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0030461800901669307	0.0	0.0	0.0	0.0
10	0.006092360180333861	0.0	0.0	0.0	0.0
11	0.006092360180333861	0.0	0.0	0.0	0.0
12	0.006092360180333861	0.0	0.0	0.0	0.0
13	0.006092360180333861	0.0	0.0	0.0	0.0
14	0.027415620811502377	0.0	0.0	0.0	0.0
15	0.027415620811502377	0.0	0.0	0.0	0.0
16	0.0396003411721701	0.0	0.0	0.0	0.0
17	0.04264652126233703	0.0	0.0	0.0	0.0
18	0.04569270135250396	0.0	0.0	0.0	0.0
19	0.04569270135250396	0.0	0.0	0.0	0.0
20	0.06092360180333861	0.0	0.0	0.0	0.0
21	0.06092360180333861	0.0	0.0	0.0	0.0
22	0.06092360180333861	0.0	0.0	0.0	0.0
23	0.0700621420738394	0.0	0.0	0.0	0.0
24	0.08529304252467405	0.0	0.0	0.0	0.0
25	0.09747776288534178	0.0	0.0	0.0	0.0
26	0.11575484342634336	0.0	0.0	0.0	0.0
27	0.11575484342634336	0.0	0.0	0.0	0.0
28	0.1188010235165103	0.0	0.0	0.0	0.0
29	0.12184720360667722	0.0	0.0	0.0	0.0
30	0.130985743877178	0.0	0.0	0.0	0.0
31	0.130985743877178	0.0	0.0	0.0	0.0
32	0.130985743877178	0.0	0.0	0.0	0.0
33	0.130985743877178	0.0	0.0	0.0	0.0
34	0.130985743877178	0.0	0.0	0.0	0.0
35	0.13403192396734495	0.0	0.0	0.0	0.0
36	0.13403192396734495	0.0	0.0	0.0	0.0
37	0.13403192396734495	0.0	0.0	0.0	0.0
38	0.1370781040575119	0.0	0.0	0.0	0.0
39	0.1370781040575119	0.0	0.0	0.0	0.0
40	0.1401242841476788	0.0	0.0	0.0	0.0
41	0.14317046423784574	0.0	0.0	0.0	0.0
42	0.14621664432801268	0.0	0.0	0.0	0.0
43	0.1584013646886804	0.0	0.0	0.0	0.0
44	0.1584013646886804	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTCC	15	0.008228415	50.19816	44
TGCACAG	15	0.008228415	50.19816	41
TTCGTGG	15	0.008228415	50.19816	15
AATTCGT	15	0.008228415	50.19816	13
CCAGTCC	20	4.044097E-4	50.19816	44
TGTTCAC	15	0.008228415	50.19816	15
CCGCACA	60	0.0	50.19816	40
TGGGGAT	15	0.008228415	50.19816	15
ATTCGTG	15	0.008228415	50.19816	14
TCCGCAC	60	0.0	50.19816	39
CGATGGA	30	9.891864E-7	50.19816	14
GGTATCG	15	0.008228415	50.19816	15
TTTGTCA	15	0.008228415	50.19816	41
CGTTCGC	15	0.008278601	50.121166	46
CAGTCCT	30	9.99762E-7	50.121166	45
TGCAATC	20	4.0749347E-4	50.121166	50
GATGCGA	30	9.99762E-7	50.121166	10
ATGCGAT	30	9.99762E-7	50.121166	11
GAATGTA	15	0.008278601	50.121166	9
GTCCTGA	30	9.99762E-7	50.121166	47
>>END_MODULE