FastQCFastQC Report
Fri 17 Jun 2016
SRR1526222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25230
Sequences flagged as poor quality0
Sequence length56
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.3765358699960365No Hit
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG470.186286167261197No Hit
TCAAAGGGGGGGCTCCTGTTTGAGCTGGTTATTGTTGCTCCTCTGAGGGTCCCTCT430.17043202536662702No Hit
TCAAAGGGGATTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.11890606420927466No Hit
TCAAAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.11890606420927466No Hit
TCAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.1109789932619897No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC280.1109789932619897No Hit
TCAAAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA260.10305192231470472No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTCTG150.00803555550.46478741
CCTTTTC204.040234E-450.16200343
GAGCACA150.00822957650.16249
CACAGGA150.00822957650.16244
AGGAGCA150.00822957650.16247
TAGTAGC150.00829501850.06187433
ATCACAG150.00829501850.06187442
AGCACAG150.00829501850.06187450
TCTCTGG150.00829501850.06187442
AAGAGTG204.1209243E-449.96215410
ACAACGA204.1209243E-449.96215412
ACAGTGT150.00836084649.9621510
ACAGTAG150.00836084649.9621529
AGTAGTA150.00836084649.9621531
AACTGGT150.00836084649.9621510
CAGTAGT150.00836084649.9621530
GAAGTGT150.00836084649.962159
GAACGCA150.00836084649.962159
AACCTGT150.00836084649.9621510
ACCTGTC150.00836084649.9621511