##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526222_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25230 Sequences flagged as poor quality 0 Sequence length 56 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.828775267538646 39.0 38.0 40.0 35.0 40.0 2 37.37403884264764 39.0 38.0 40.0 33.0 40.0 3 35.9610384462941 39.0 37.0 39.0 26.0 40.0 4 35.8516845025763 39.0 37.0 40.0 24.0 40.0 5 35.42366230677764 39.0 36.0 39.0 22.0 40.0 6 36.72358303606817 39.0 36.0 39.0 31.0 40.0 7 37.35001981767737 39.0 38.0 39.0 33.0 40.0 8 37.25885850178359 39.0 38.0 39.0 33.0 40.0 9 37.208838684106226 39.0 38.0 39.0 33.0 40.0 10 36.724137931034484 39.0 37.0 39.0 31.0 40.0 11 35.94229092350376 38.0 35.0 39.0 30.0 40.0 12 34.358898137138326 38.0 33.0 39.0 22.0 40.0 13 34.16781609195402 38.0 33.0 39.0 22.0 40.0 14 34.27970669837495 38.0 33.0 39.0 22.0 40.0 15 34.198850574712644 38.0 33.0 39.0 22.0 40.0 16 33.56357510899723 38.0 31.0 39.0 21.0 40.0 17 33.5640903686088 38.0 31.0 39.0 21.0 40.0 18 32.810225921522 37.0 30.0 39.0 18.0 40.0 19 33.78216409036861 37.0 31.0 39.0 23.0 40.0 20 33.954300435988905 37.0 31.0 39.0 23.0 40.0 21 34.77277051129607 38.0 33.0 39.0 25.0 40.0 22 34.42251288149029 38.0 33.0 39.0 23.0 40.0 23 35.007292905271505 38.0 34.0 39.0 26.0 40.0 24 34.60011890606421 38.0 33.0 39.0 24.0 40.0 25 34.87879508521601 38.0 33.0 39.0 26.0 40.0 26 35.64292508917955 38.0 35.0 39.0 29.0 40.0 27 35.53067776456599 38.0 35.0 39.0 28.0 40.0 28 35.59690844233056 38.0 35.0 39.0 29.0 40.0 29 35.41010701545779 38.0 35.0 39.0 28.0 40.0 30 35.444074514466905 38.0 35.0 39.0 28.0 40.0 31 35.905945303210466 38.0 35.0 39.0 30.0 40.0 32 35.630677764565995 38.0 35.0 39.0 29.0 40.0 33 35.83725723345224 38.0 35.0 39.0 30.0 40.0 34 35.80951248513674 38.0 35.0 39.0 30.0 40.0 35 35.71759809750297 38.0 35.0 39.0 30.0 40.0 36 35.04724534284582 38.0 35.0 39.0 29.0 40.0 37 34.79472849782005 38.0 35.0 39.0 29.0 40.0 38 34.49980182322632 38.0 34.0 39.0 28.0 40.0 39 33.98945699564011 38.0 33.0 39.0 26.0 40.0 40 30.68545382481173 35.0 27.0 39.0 10.0 40.0 41 27.117043202536664 32.0 16.0 38.0 6.0 39.0 42 31.743321442726913 35.0 30.0 38.0 17.0 39.0 43 30.22929052715022 35.0 28.0 38.0 7.0 39.0 44 30.05818470075307 35.0 28.0 38.0 6.0 39.0 45 31.345937376139517 35.0 30.0 38.0 11.0 39.0 46 31.02718985334919 35.0 29.0 39.0 4.0 40.0 47 30.496908442330557 35.0 28.0 38.0 4.0 40.0 48 30.73602853745541 35.0 28.0 38.0 4.0 40.0 49 27.893182718985337 35.0 17.0 38.0 4.0 40.0 50 30.274237019421324 35.0 27.0 38.0 4.0 40.0 51 30.546373365041617 36.0 29.0 39.0 4.0 40.0 52 30.47395957193817 35.0 29.0 39.0 4.0 40.0 53 30.06286167261197 35.0 28.0 39.0 4.0 40.0 54 28.870471660721364 35.0 23.0 39.0 4.0 40.0 55 29.32251288149029 35.0 27.0 39.0 4.0 40.0 56 27.946967895362665 35.0 17.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.2043581938252288 1 2 1.5915065530104826 1 3 3.236831578250083 1 4 3.992266665944534 1 5 4.354344873181084 1 6 1.9517067962545909 1 7 1.5484923169509486 1 8 1.7197034146681887 1 9 1.8719441298790187 1 10 1.9349175075018294 1 11 -3.2115076555268622 1 12 -11.738370227404271 1 13 -7.547689030318509 1 14 -6.376886964551428 1 15 -7.274063749132029 1 16 -22.868497364022183 1 17 -4.84321623022003 1 18 -14.227573514752315 1 19 -6.4976806131376605 1 20 -6.253061223619206 1 21 -4.518402663988624 1 22 -2.3966938238157773 1 23 -2.6273832763143226 1 24 -2.941754412138085 1 25 -2.72480702053441 1 26 2.308626963707056 1 27 2.0292015437109754 1 28 2.52230008566665 1 29 2.026763967982056 1 30 2.287338995811645 1 31 2.2112356630180727 1 32 1.961551760878045 1 33 2.6926291703865317 1 34 1.429805690616675 1 35 2.133651432819228 1 36 2.2325918608452753 1 37 2.9703523076750926 1 38 3.5123939057239966 1 39 4.427207275541981 1 40 7.790758768507139 1 41 -10.228430972767987 1 42 -9.923208166452412 1 43 -18.388621440149002 1 44 -22.269252439654476 1 45 -11.26109774243438 1 46 -16.743336004936474 1 47 -23.312241941741412 1 48 -14.822312826444955 1 49 -20.710591383215665 1 50 -13.678806908817126 1 51 -10.12621661435346 1 52 -10.047392845211515 1 53 -10.087284507136474 1 54 -9.006791929027312 1 55 -9.304364399868156 1 56 -4.025333785502944 2 1 1.5548086442756812 2 2 2.308623670127595 2 3 4.408002749421257 2 4 4.596771170449038 2 5 4.597588116424326 2 6 2.1454004899482797 2 7 1.889032857491486 2 8 2.06655026151504 2 9 1.6467189046537882 2 10 0.9997823723666954 2 11 1.7659698219506197 2 12 -2.3239558129898583 2 13 -2.2521935348230144 2 14 3.0699598822954215 2 15 2.253864178795901 2 16 1.6143855188607006 2 17 3.3513783643745683 2 18 -1.9311771183559188 2 19 2.539256323799279 2 20 1.7640558934979111 2 21 1.8059216603356987 2 22 3.0735764464544957 2 23 2.314058165127115 2 24 1.0591464887628135 2 25 2.4787965830691974 2 26 2.0671855222656177 2 27 3.2084808229902535 2 28 2.29076855413512 2 29 2.8321693733874582 2 30 2.1422939507666 2 31 1.5950194468018566 2 32 1.8516418509681358 2 33 1.2295661073234712 2 34 1.8964723572833435 2 35 1.757074856242653 2 36 1.8866459148993329 2 37 3.0496315869543693 2 38 2.749330842660939 2 39 3.374955023289729 2 40 5.667335345083714 2 41 -0.897800342137355 2 42 -3.453838797083044 2 43 -11.759792611320176 2 44 -13.51339658379862 2 45 -2.568304949641586 2 46 -7.277570239170707 2 47 -17.515845545345016 2 48 -6.125015529147657 2 49 -10.610591383215667 2 50 -5.284212314222533 2 51 -0.22171210984895495 2 52 0.3832377854191158 2 53 -0.1944917143436804 2 54 0.44996482772944546 2 55 0.41185181634806156 2 56 4.6710626108934505 3 1 1.8567391462061877 3 2 2.6676970292009585 3 3 4.655879197297708 3 4 4.958933332611203 3 5 5.582916301752512 3 6 3.118373462921255 3 7 2.4342066026652347 3 8 2.6101796051443813 3 9 2.481467939402826 3 10 2.249203221787546 3 11 2.7551590111398028 3 12 3.480677391643347 3 13 3.4427871601576783 3 14 3.299303511639046 3 15 3.7449838699155933 3 16 5.055312159787341 3 17 5.556783769779969 3 18 4.967664580485778 3 19 5.154700339243291 3 20 3.9231292525712718 3 21 3.7673116217256606 3 22 4.174734747612796 3 23 3.805950057019011 3 24 4.377293206909535 3 25 3.1228120270846347 3 26 3.7181507732308674 3 27 2.948249162758593 3 28 3.4413477047142678 3 29 3.4553353965534868 3 30 3.2635294720021193 3 31 2.782664234446642 3 32 3.3234565227828057 3 33 2.559295837053199 3 34 3.1536152144262033 3 35 2.228889528057323 3 36 3.7754490037024198 3 37 4.360828498151285 3 38 3.621917715247811 3 39 5.608159656494365 3 40 9.309806387554758 3 41 9.404902360565348 3 42 6.129172785928539 3 43 1.7637595122319496 3 44 2.92598565558362 3 45 6.143664162327525 3 46 4.518568756968289 3 47 -1.3598609893604596 3 48 4.891972887840762 3 49 6.417980045355765 3 50 5.0926216626114424 3 51 5.711878623741779 3 52 5.1716547738361065 3 53 3.031763111911147 3 54 4.950350928115547 3 55 2.5956356001318426 3 56 3.969904309735149 4 1 1.5210248604918988 4 2 2.0248398863438126 4 3 4.181276022694533 4 4 3.7867111103889783 4 5 4.771011539847748 4 6 2.0905956851434766 4 7 1.6818256502842814 4 8 1.830814525779303 4 9 1.9552774632123473 4 10 1.9960286186129466 4 11 2.2051590111398056 4 12 3.7060742170401753 4 13 4.280088747459267 4 14 3.66755747989302 4 15 2.54815847309019 4 16 4.814835969311147 4 17 5.03456154755775 4 18 5.811315374136573 4 19 4.657874942417891 4 20 3.646938776380793 4 21 4.64826400267804 4 22 4.803306176184222 4 23 4.20595005701901 4 24 4.297134476750806 4 25 3.0140818683544808 4 26 1.4530714081515015 4 27 1.5125348770443097 4 28 1.905633418999983 4 29 2.1212084124264976 4 30 2.8540056624783148 4 31 1.6167912185736313 4 32 1.6226628719891565 4 33 1.9592958370531974 4 34 0.8409168017277864 4 35 1.9169847661525594 4 36 3.638147416400834 4 37 3.5203523076750898 4 38 4.006838350168444 4 39 4.904985053319763 4 40 7.118536546284915 4 41 10.24934680500979 4 42 5.92679183354759 4 43 7.800267448739884 4 44 6.852969782567747 4 45 5.666680035343397 4 46 5.623330661730197 4 47 5.782202502703033 4 48 5.5499093957772665 4 49 8.306075283450998 4 50 3.51008198007176 4 51 4.912672274535428 4 52 5.391496043677375 4 53 5.734937715085746 4 54 7.165430293194909 4 55 5.695635600131844 4 56 6.719110658941499 5 1 2.1138820033490404 5 2 2.8962684577723863 5 3 4.7701649115834215 5 4 5.130361904039773 5 5 5.697202016038226 5 6 2.9183734629212523 5 7 2.6342066026652304 5 8 2.5816081765729564 5 9 2.681467939402829 5 10 2.6492032217875447 5 11 3.640873296854089 5 12 5.394963105929058 5 13 5.071358588729112 5 14 5.470732083067617 5 15 5.5449838699155904 5 16 5.7124550169302 5 17 6.0710694840656885 5 18 6.453378866200065 5 19 5.268986053529005 5 20 4.837414966856983 5 21 4.8815973360113745 5 22 5.574734747612794 5 23 4.977378628447582 5 24 5.320150349766678 5 25 4.665669169941779 5 26 3.5752936303737215 5 27 3.776820591330022 5 28 3.6127762761428386 5 29 3.4267639679820547 5 30 3.9206723291449777 5 31 3.039807091589502 5 32 2.923456522782807 5 33 3.0164386941960544 5 34 2.7536152144261976 5 35 3.057460956628752 5 36 4.289734717988132 5 37 3.503685641008424 5 38 4.879060572390664 5 39 5.408159656494362 5 40 8.82409210184047 5 41 11.090616646279631 5 42 6.9863156430714 5 43 5.478045226517661 5 44 7.325985655583622 5 45 5.6293784480418125 5 46 7.0328544712540015 5 47 6.582996153496683 5 48 6.377687173555042 5 49 9.27512290249862 5 50 6.692621662611444 5 51 6.8547357665989175 5 52 6.885940488121815 5 53 7.717477397625434 5 54 9.007493785258404 5 55 7.281349885846133 5 56 8.541332881163722 6 1 1.4932470827141202 6 2 1.7748398863438126 6 3 3.4590538004723044 6 4 4.5089333326112 6 5 4.74323376206997 6 6 2.2850401295879195 6 7 2.0429367613953886 6 8 2.2474811924459672 6 9 2.34416635210124 6 10 2.3849175075018323 6 11 3.0384923444731413 6 12 3.789407550373504 6 13 4.66897763634816 6 14 3.97311303544857 6 15 4.2148251397568615 6 16 4.592613747088926 6 17 5.923450436446636 6 18 5.561315374136573 6 19 4.574541609084562 6 20 4.480272109714129 6 21 3.64826400267804 6 22 3.6366395095175577 6 23 3.3448389459078953 6 24 3.6860233656396915 6 25 3.625192979465588 6 26 2.2030714081515015 6 27 0.9569793214887525 6 28 2.1834111967777616 6 29 2.287875079093169 6 30 2.770672329144979 6 31 2.1167912185736313 6 32 2.344885094211378 6 33 1.8481847259420832 6 34 2.174250135061122 6 35 2.0558736550414523 6 36 2.888147416400834 6 37 3.492574529897311 6 38 2.367949461279551 6 39 3.51609616443087 6 40 7.090758768507136 6 41 4.054902360565347 6 42 4.0656807224364755 6 43 6.828045226517663 6 44 4.936303115901076 6 45 3.9444578131211756 6 46 5.039997328396861 6 47 5.976646947147476 6 48 5.744353840221709 6 49 9.139408616784333 6 50 5.70452642451621 6 51 3.773783385646542 6 52 3.1692738214551532 6 53 3.09604882619686 6 54 3.6932080709726876 6 55 3.140080044576287 6 56 5.219110658941499 7 1 1.8004366251977828 7 2 2.7307222392849866 7 3 4.570164911583419 7 4 4.876580391434729 7 5 5.266109579063439 7 6 2.294844051156552 7 7 2.2896687875391777 7 8 2.0824485127074084 7 9 1.979787267133915 7 10 2.4878586839724193 7 11 2.9845707758456896 7 12 4.947904282399648 7 13 4.732703126544237 7 14 3.5270346040760217 7 15 4.218093113613072 7 16 5.633463420291541 7 17 5.021489652132914 7 18 5.221446093090822 7 19 3.033691935881947 7 20 3.7449779920670707 7 21 2.981597336011376 7 22 3.097423823243048 7 23 3.4118324099601836 7 24 2.6680495094305456 7 25 3.0908792539753946 7 26 1.8811759833148969 7 27 2.6448878182207807 7 28 1.655633418999983 7 29 2.8973522032761707 7 30 2.638319387968508 7 31 2.811235663018074 7 32 1.241943917740791 7 33 2.6651781899943714 7 34 1.7788252984598145 7 35 1.323847511250598 7 36 3.0678859784923347 7 37 3.6330974057143095 7 38 3.508472337096549 7 39 4.0585798245615905 7 40 7.571150925369885 7 41 0.7166670664476982 7 42 5.696399676684841 7 43 6.578045226517663 7 44 7.268002462306306 7 45 5.94282382619307 7 46 6.966467916632158 7 47 6.72501296021937 7 48 6.901216585319748 7 49 9.212938028549036 7 50 6.5329577970652295 7 51 5.940450052313206 7 52 4.9094698998865205 7 53 5.375460590902744 7 54 6.8990904239138615 7 55 4.754459129543612 7 56 4.735450528222543 8 1 1.7104187998858364 8 2 2.039991401495328 8 3 4.358043699462208 8 4 4.334690908368778 8 5 5.081617600453811 8 6 2.088070432618224 8 7 2.166674135132766 8 8 2.4015215964863756 8 9 1.8719441298790187 8 10 1.8015841741684966 8 11 2.7733408293216257 8 12 4.776781287747241 8 13 4.4341291514996755 8 14 4.556446368781906 8 15 3.8713907963225154 8 16 4.973926878402061 8 17 5.120420133416339 8 18 5.005759818581016 8 19 4.9356527201956695 8 20 4.222696352138371 8 21 3.905839760253798 8 22 4.591184964063011 8 23 3.660495511564463 8 24 4.037033466649795 8 25 3.0267081309807438 8 26 2.599536054616145 8 27 1.8988985134079428 8 28 0.5192697826363499 8 29 2.487370028588117 8 30 1.7630965715692213 8 31 0.6597205115029254 8 32 2.5797335790598623 8 33 1.898689776447135 8 34 1.6237450845560701 8 35 1.576075675243473 8 36 1.3780464062998234 8 37 1.4976250349478164 8 38 1.842696936027032 8 39 2.415086063420773 8 40 5.3331830109313785 8 41 -2.8920673364043488 8 42 3.419216075971832 8 43 5.956833105305538 8 44 5.842868772466737 8 45 4.66920528786865 8 46 5.774845813245346 8 47 5.542303512804043 8 48 3.729202325070194 8 49 7.450014677390396 8 50 4.696950666940452 8 51 4.758631870495027 8 52 4.207152609333942 8 53 4.679382159530196 8 54 6.450783828548445 8 55 6.52896893346518 8 56 6.244363184194025 9 1 1.7407218301888676 9 2 2.767264128768055 9 3 4.721680063098567 9 4 4.880145453823324 9 5 4.8391933580295685 9 6 2.482009826557622 9 7 2.5303104987691256 9 8 2.3712185661833445 9 9 2.6295198874547765 9 10 2.7712811438654654 9 11 3.2884923444731413 9 12 4.594963105929061 9 13 4.706856424226949 9 14 4.9200827324182725 9 15 4.871390796322515 9 16 4.94362384809903 9 17 5.726480739476941 9 18 6.460305273126476 9 19 5.39019817474113 9 20 5.071181200623222 9 21 4.724021578435618 9 22 4.682094054972097 9 23 4.508980360049314 9 24 4.430972860589186 9 25 3.450950555223166 9 26 2.751051206131301 9 27 2.2625348770443097 9 28 3.337451600818163 9 29 3.638885180103266 9 30 2.91461172308437 9 31 3.174872026654441 9 32 2.943369942696229 9 33 3.3229322006895643 9 34 2.62374508455607 9 35 1.6366817358495283 9 36 3.0144100426634566 9 37 3.7400492773720586 9 38 3.630575723905814 9 39 4.687813336148039 9 40 8.333183010931378 9 41 4.7745993302623155 9 42 6.661640318396074 9 43 8.44168159015403 9 44 8.11559604519401 9 45 6.911629530292892 9 46 7.25969429809383 9 47 7.057455027955559 9 48 6.335262931130803 9 49 9.722741950117669 9 50 7.303011273001054 9 51 6.970753082616238 9 52 7.449576851758184 9 53 7.16423064437868 9 54 8.481086858851477 9 55 8.07442347891972 9 56 7.577696517527354 10 1 1.7043581938252288 10 2 2.1581732196771455 10 3 4.370164911583416 10 4 4.558933332611197 10 5 4.787678206514414 10 6 2.185040129587925 10 7 1.9818256502842786 10 8 2.2863700813348586 10 9 1.2386107965456858 10 10 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115 32 1.094885094211378 115 33 -0.21461720642506066 115 34 0.9834288790224761 115 35 -1.0866384222532375 115 36 2.2673744695409255 115 37 0.4080334670953789 115 38 1.3486257897819698 115 39 1.989526116121695 115 40 6.424092101840472 115 41 4.587511056217522 115 42 0.7999802393446878 115 43 -6.834998251743208 115 44 -8.572150990379114 115 45 -1.9842861482314795 115 46 -6.753480932472705 115 47 -5.289053535944312 115 48 -6.117965000357998 115 49 -15.13233051365045 115 50 -5.313589517512778 115 51 -4.146506469425923 115 52 -4.087972555356444 115 53 -3.3061250868466168 115 54 -10.002444102940355 115 55 -5.02175570421598 115 56 -2.536927988401498 116 1 1.7325633220303587 116 2 2.3709937324976593 116 3 4.5317033731218785 116 4 4.68201025568812 116 5 5.315883334719544 116 6 2.8106811552289486 116 7 2.002338470797099 116 8 2.5145752095399843 116 9 2.2309184888533764 116 10 2.6733790459633724 116 11 -0.6217640657832746 116 12 -11.597344586378632 116 13 -5.029740312369793 116 14 -3.1358613235257877 116 15 -6.571499646567926 116 16 -1.7172153127401337 116 17 -0.8970623840661816 116 18 -2.583983771162572 116 19 -0.5387062541633014 116 20 -1.4812663518243312 116 21 4.443135797549836 116 22 4.380229253107302 116 23 4.013642364711316 116 24 3.0300404596567887 116 25 3.0931416974143104 116 26 2.9445243996044894 116 27 2.1471502616596965 116 28 3.2710180343845963 116 29 1.8113793525974415 116 30 3.2899030983757456 116 31 3.272774124556534 116 32 3.3256543249806114 116 33 3.0362189139762776 116 34 2.8964723572833435 116 35 3.038779637947435 116 36 4.06336109161451 116 37 4.6036856410084255 116 38 4.594445187775278 116 39 5.563104711439415 116 40 9.693322871071238 116 41 5.99721005287304 116 42 1.304996961752714 116 43 -11.57580092732849 116 44 -9.125662696064733 116 45 1.9722355908989542 116 46 -0.363848825449292 116 47 0.7159631864637142 116 48 0.5776871735550451 116 49 -10.572129844754127 116 50 0.08273155272133437 116 51 2.4404500523132064 116 52 2.393632795814124 116 53 0.7691257492737833 116 54 -9.845253467488853 116 55 2.426404830901074 116 56 2.095179035009874 117 1 0.38213597160301305 117 2 1.135950997454927 117 3 3.181276022694533 117 4 3.314488888166757 117 5 3.854344873181084 117 6 1.7294845740323694 117 7 0.8484923169509457 117 8 1.1641478591126386 117 9 0.7052774632123473 117 10 0.8571397297240537 117 11 -1.6003965444157515 117 12 -10.460592449626496 117 13 -6.914355696985176 117 14 -6.221331408995873 117 15 -7.7851748602431385 117 16 -12.768497364022185 117 17 -12.798771785775585 117 18 -14.27201795919676 117 19 -5.897680613137663 117 20 -5.79750566806365 117 21 0.7593751137891545 117 22 1.6921950650731077 117 23 0.31706116813012386 117 24 -1.0639766343603085 117 25 -0.45814035386774066 117 26 1.9530714081515015 117 27 2.429201543710974 117 28 2.2667445301110973 117 29 2.4267639679820547 117 30 2.409561218033865 117 31 1.9779023296847384 117 32 2.261551760878042 117 33 1.5148513926087546 117 34 0.9520279128389006 117 35 0.05587365504145225 117 36 1.3881474164008338 117 37 1.4370189743417612 117 38 1.9790605723906651 117 39 2.960540608875313 117 40 7.090758768507136 117 41 4.638235693898679 117 42 -0.9898748331190781 117 43 -8.36639921792678 117 44 -10.369252439654478 117 45 1.0833467020100649 117 46 -1.1544471160475815 117 47 -2.1900197195191886 117 48 -2.811201715333844 117 49 -5.999480272104556 117 50 -4.8788069088171255 117 51 -6.392883281020126 117 52 -6.747392845211515 117 53 -7.098395618247583 117 54 -8.47345859569398 117 55 -5.582142177645935 117 56 -3.2808893410585007 118 1 0.5971118170136407 118 2 1.3074485819959847 118 3 3.0919040420182 118 4 3.5850202891329417 118 5 4.093475307963693 118 6 1.7270691150951691 118 7 1.1745792734726876 118 8 1.3791237045232663 118 9 1.2342629704587296 118 10 1.9175262031540043 118 11 -1.131797510599327 118 12 -5.839819502766591 118 13 -10.414355696985176 118 14 -7.617466674696356 118 15 -6.123339111450871 118 16 -11.514874175616388 118 17 -10.721477099785247 118 18 -9.747863369824778 118 19 -3.9049269899492565 118 20 -3.1066844120250003 118 21 0.6120321186200712 118 22 1.194610524010308 118 23 1.5972544048450956 118 24 0.18723109510829516 118 25 0.12156979105979104 118 26 0.9144240651563322 118 27 0.17557835530517707 118 28 0.24258994073911566 118 29 0.6441552723298827 118 30 0.8250201552319325 118 31 -0.2322425978514886 118 32 -5.165984471006013 118 33 0.002774097922760177 118 34 0.20082018337029695 118 35 -0.5648992918184561 118 36 0.35433099128005807 118 37 0.9297725975301674 118 38 1.087756224564579 118 39 2.2503956813390857 118 40 6.424092101840472 118 41 4.239684969260999 118 42 -1.547845847611832 118 43 -8.704563469134513 118 44 -9.876498816466071 118 45 -7.332112235188003 118 46 -11.840437454211834 118 47 -13.549923101161703 118 48 -13.422312826444955 118 49 -11.958417470172188 118 50 -9.357067778382344 118 51 -8.146506469425923 118 52 -7.870581251008616 118 53 -6.784385956411835 118 54 -12.741574537722965 118 55 -6.02175570421598 118 56 -2.232580162314541 119 1 0.6281677176347529 119 2 1.3819827434866667 119 3 3.284450625869134 119 4 3.520838094515959 119 5 4.211487730323938 119 6 1.6421829867307807 119 7 1.2056351740937998 119 8 1.2673224622872397 119 9 1.1576584155933034 119 10 1.8492032217875476 119 11 -3.56865051266972 119 12 -7.071703560737607 119 13 -7.747689030318508 119 14 -5.729267916932379 119 15 -8.62644470151298 119 16 -10.93516403068885 119 17 -15.671787658791455 119 18 -15.279954467133269 119 19 -8.111966327423374 119 20 -8.591156461714444 119 21 -0.8041169497029088 119 22 -0.053836680958632144 119 23 0.015473866542819792 119 24 -0.4608020311857004 119 25 -0.02956892529631716 119 26 0.9848174398975331 119 27 0.8339634484728791 119 28 0.5365857999523627 119 29 0.5220020632201496 119 30 0.9016247100973587 119 31 -0.37924052745811565 119 32 -4.476543477217195 119 33 -0.32641844866108727 119 34 -0.4368609760499922 119 35 -0.09491999575219978 119 36 1.0706870989405175 119 37 1.484638021960805 119 38 1.5266796200097161 119 39 2.674826323161028 119 40 6.471711149459523 119 41 2.614426170089157 119 42 -2.966065309309556 119 43 -10.207669059196622 119 44 -10.32163339203543 119 45 -3.2182405995772356 119 46 -14.948097909698376 119 47 -13.483670513169983 119 48 -9.89850330263543 119 49 -11.801067573691856 119 50 -8.640711670721888 119 51 -6.392883281020126 119 52 -5.96167855949723 119 53 -7.511094030945998 119 54 -13.735363357598741 119 55 -6.232935828439583 119 56 -2.7729528331219946 120 1 -0.5067529172858798 120 2 0.4692843307882555 120 3 2.5146093560278615 120 4 2.7033777770556426 120 5 3.298789317625527 120 6 1.062817907365698 120 7 0.2929367613953886 120 8 0.4974811924459672 120 9 0.3719441298790187 120 10 1.0793619519462752 120 11 -4.989285433304637 120 12 -15.071703560737607 120 13 -13.192133474762954 120 14 -12.887998075662537 120 15 -10.507397082465364 120 16 -17.26849736402218 120 17 -17.132105119108918 120 18 -16.216462403641202 120 19 -7.8421250575821055 120 20 -13.075283445841428 120 21 -7.462847108433067 120 22 -3.863360490482446 120 23 -4.516272165203212 120 24 -10.89730996769364 120 25 -3.1803625760899656 120 26 1.73084918592928 120 27 1.5958682103776454 120 28 1.5445223078888688 120 29 1.8156528568709405 120 30 -0.36821655974390666 120 31 -0.744319892537483 120 32 -9.794003794677511 120 33 -1.262926385169024 120 34 -1.2146387538277708 120 35 -1.4441263449585477 120 36 -0.22296369471028044 120 37 -0.0074254701026887915 120 38 -0.076494983164892 120 39 0.7383183866530914 120 40 5.035203212951586 120 41 -2.361764306101321 120 42 -8.212097055341303 120 43 -12.644176995704559 120 44 -17.535919106321145 120 45 -8.694431075767714 120 46 -19.265558227158692 120 47 -16.8011308306303 120 48 -14.311201715333844 120 49 -18.110591383215667 120 50 -13.82325135326157 120 51 -13.559549947686794 120 52 -14.080726178544849 120 53 -12.431728951580919 120 54 -17.417903040138423 120 55 -11.804364399868156 120 56 -9.7808893410585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 174.0 5 6.0 6 12.0 7 16.0 8 28.0 9 38.0 10 28.0 11 26.0 12 23.0 13 20.0 14 32.0 15 55.0 16 70.0 17 70.0 18 91.0 19 141.0 20 186.0 21 221.0 22 242.0 23 305.0 24 409.0 25 447.0 26 468.0 27 545.0 28 664.0 29 790.0 30 934.0 31 1067.0 32 1253.0 33 1410.0 34 1701.0 35 1957.0 36 2506.0 37 3368.0 38 3581.0 39 2340.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.47284978200554 0.380499405469679 0.12286959968291716 0.023781212841854936 8 99.51248513674199 0.3686087990487515 0.08323424494649227 0.0356718192627824 9 97.15814506539834 1.7796274276654775 0.6539833531510106 0.4082441537851764 10 29.159730479587793 49.74237019421324 11.022592152199762 10.075307173999207 11 26.22671422909235 36.97582243361078 20.848196591359493 15.949266745937377 12 37.44351961950059 16.361474435196193 21.95402298850575 24.240982956797463 13 23.13852985488859 18.269764491317105 26.87732931567679 31.714376338117518 14 23.638525564803807 24.894966309948472 26.325802615933412 25.14070550931431 15 27.660335373122468 21.918461416196454 26.571564809663833 23.849638401017245 16 26.961970099536025 23.59915929729944 26.438513701074672 23.000356902089862 17 25.097106619104242 24.990091161315895 24.55410225921522 25.358699960364646 18 21.60919540229885 28.398731668648438 24.526357510899725 25.46571541815299 19 20.860283322090392 33.23677631840006 23.16574739097655 22.737192968532995 20 22.99758003729123 28.654738763042015 23.826714801444044 24.520966398222715 21 23.995243757431627 26.187078874355922 24.38367023384859 25.43400713436385 22 24.7205707491082 26.00871977804201 24.328180737217597 24.942528735632184 23 23.301625049544196 30.031708283789136 24.28458184700753 22.382084819659138 24 22.826000792707095 30.396353547364246 25.854141894569953 20.9235037653587 25 24.276654776060248 29.25485533095521 26.821244550138722 19.647245342845817 26 21.783590963139122 28.232263178755453 30.68569163694015 19.29845422116528 27 21.52295543827626 23.95143049878973 34.784333955001784 19.741280107932223 28 20.161930465153198 24.630893792665503 37.426575646928086 17.780600095253217 29 17.86535564189993 23.895805249385457 39.25541194195544 18.983427166759178 30 15.440418615713947 23.523348925711566 43.51066360104654 17.525568857527947 31 14.466381224230892 23.299238820171265 45.317951157627654 16.916428797970188 32 15.228084093613644 22.332407774692584 47.58825862752876 14.851249504165015 33 14.447355883754168 21.268064157535335 50.23026838176909 14.0543115769414 34 12.564926053685422 21.89048808532572 53.261171246183736 12.283414614805123 35 12.634792261338406 20.956232159847765 54.999207104345075 11.409768474468759 36 12.240285487708167 22.057890563045202 53.501189532117365 12.200634417129262 37 12.480635551142004 20.568023833167825 54.47070506454816 12.480635551142004 38 13.00321901204149 21.841592814847196 54.719230616381196 10.43595755673012 39 12.501489691335955 21.781273586779488 52.76288086441823 12.954355857466332 40 9.569282403707847 17.1727665015183 47.51478344254435 25.743167652229502 41 8.869064287714526 15.7418890266832 44.757370884506145 30.63167580109613 42 11.228821965639012 23.009165575526723 53.94595881442685 11.816053644407413 43 10.305100905768313 19.394565390116004 53.3688225011918 16.931511202923883 44 10.847309086563358 19.742660239811975 51.55160737760427 17.858423296020394 45 12.29198935620954 22.18912585885063 53.87426029627864 11.644624488661186 46 12.314840554386244 22.37401215201938 53.39343155553791 11.91771573805647 47 11.241078509119747 22.724028548770818 53.51308485329104 12.521808088818398 48 12.635966653433902 23.33465660976578 53.366415244144505 10.662961492655816 49 9.947018284667172 19.599251085527627 47.4485121300243 23.005218499780902 50 11.145953240979638 24.915651172944866 52.93136982495138 11.00702576112412 51 11.87343482927217 25.10235719680407 52.04913145446596 10.975076519457804 52 11.655418816990869 24.942437475188566 51.869789599047245 11.532354108773323 53 11.86534873565956 24.97320471596999 52.21706164900163 10.944384899368822 54 11.075798506276815 24.213411727316064 49.31670109645638 15.39408866995074 55 11.809584359850591 25.598029086863228 51.740443455455775 10.851943097830405 56 12.222531072548943 25.334551086050112 50.98280586109677 11.460111980304173 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.5 5 3.0 6 4.0 7 5.0 8 28.5 9 52.0 10 97.0 11 142.0 12 186.5 13 242.0 14 253.0 15 311.5 16 370.0 17 461.0 18 552.0 19 535.0 20 518.0 21 622.5 22 947.0 23 1167.0 24 1344.5 25 1522.0 26 1673.5 27 1825.0 28 1848.5 29 1921.0 30 1970.0 31 1726.5 32 1483.0 33 1218.5 34 954.0 35 836.5 36 719.0 37 605.5 38 471.0 39 450.0 40 474.0 41 498.0 42 501.0 43 504.0 44 539.5 45 575.0 46 610.5 47 638.0 48 630.0 49 643.5 50 657.0 51 679.5 52 702.0 53 681.0 54 721.0 55 782.0 56 725.0 57 668.0 58 717.5 59 767.0 60 713.0 61 659.0 62 626.0 63 532.5 64 472.0 65 396.0 66 320.0 67 303.5 68 287.0 69 260.0 70 233.0 71 173.5 72 92.0 73 70.0 74 53.0 75 36.0 76 33.0 77 30.0 78 20.5 79 8.0 80 5.0 81 3.0 82 1.0 83 2.0 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.03170828378913991 14 0.0 15 0.2536662703131193 16 0.05152596115735236 17 0.0 18 0.0 19 0.11494252873563218 20 0.09116131589377725 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.11494252873563218 28 0.13476020610384462 29 0.03170828378913991 30 0.015854141894569955 31 0.023781212841854936 32 0.07927070947284978 33 0.16646848989298454 34 0.0356718192627824 35 0.023781212841854936 36 0.03963535473642489 37 0.21799445105033688 38 0.26555687673404677 39 0.2259215219976219 40 0.8006341656757829 41 0.9235037653587 42 0.1070154577883472 43 0.22988505747126436 44 0.5033690051525961 45 0.20214030915576697 46 0.19421323820848196 47 0.03963535473642489 48 0.15854141894569956 49 0.5033690051525961 50 0.14665081252477208 51 0.2893380895759017 52 0.15854141894569956 53 0.15457788347205706 54 0.22988505747126436 55 0.2536662703131193 56 0.186286167261197 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 25230.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.72334522393976 #Duplication Level Percentage of deduplicated Percentage of total 1 86.30724941114892 65.354736424891 2 7.767600104684637 11.763773285770908 3 2.486260141324261 5.648038049940547 4 1.271918345982727 3.8525564803804992 5 0.6647474483119602 2.5168450257629806 6 0.41350431824129813 1.87871581450654 7 0.2721800575765506 1.4427269124058661 8 0.21983773881182936 1.3317479191438764 9 0.17796388380005235 1.2128418549346016 >10 0.41350431824129813 4.621482362267142 >50 0.005234231876472128 0.3765358699960365 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCAAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95 0.3765358699960365 No Hit TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 47 0.186286167261197 No Hit TCAAAGGGGGGGCTCCTGTTTGAGCTGGTTATTGTTGCTCCTCTGAGGGTCCCTCT 43 0.17043202536662702 No Hit TCAAAGGGGATTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.11890606420927466 No Hit TCAAAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.11890606420927466 No Hit TCAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28 0.1109789932619897 No Hit TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 28 0.1109789932619897 No Hit TCAAAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 26 0.10305192231470472 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.011890606420927468 0.0 0.0 0.0 0.0 11 0.015854141894569955 0.0 0.0 0.0 0.0 12 0.019817677368212445 0.0 0.0 0.0 0.0 13 0.023781212841854936 0.0 0.0 0.0 0.0 14 0.023781212841854936 0.0 0.0 0.0 0.0 15 0.03963535473642489 0.0 0.0 0.0 0.0 16 0.03963535473642489 0.0 0.0 0.0 0.0 17 0.04359889021006738 0.0 0.0 0.0 0.0 18 0.04359889021006738 0.0 0.0 0.0 0.0 19 0.04359889021006738 0.0 0.0 0.0 0.0 20 0.055489496630994846 0.0 0.0 0.0 0.0 21 0.06738010305192231 0.0 0.0 0.0 0.0 22 0.06738010305192231 0.0 0.0 0.0 0.0 23 0.07927070947284978 0.0 0.0 0.0 0.0 24 0.09116131589377725 0.0 0.0 0.0 0.0 25 0.1070154577883472 0.0 0.0 0.0 0.0 26 0.12683313515655964 0.0 0.0 0.0 0.0 27 0.13872374157748713 0.0 0.0 0.0 0.0 28 0.1426872770511296 0.0 0.0 0.0 0.0 29 0.1466508125247721 0.0 0.0 0.0 0.0 30 0.15854141894569956 0.0 0.0 0.0 0.0 31 0.16250495441934204 0.0 0.0 0.0 0.0 32 0.16250495441934204 0.0 0.0 0.0 0.0 33 0.16646848989298454 0.0 0.0 0.0 0.0 34 0.16646848989298454 0.0 0.0 0.0 0.0 35 0.17043202536662702 0.0 0.0 0.0 0.0 36 0.18628616726119698 0.0 0.0 0.0 0.0 37 0.18628616726119698 0.0 0.0 0.0 0.0 38 0.18628616726119698 0.0 0.0 0.0 0.0 39 0.19421323820848196 0.0 0.0 0.0 0.0 40 0.19421323820848196 0.0 0.0 0.0 0.0 41 0.19421323820848196 0.0 0.0 0.0 0.011890606420927468 42 0.19817677368212447 0.0 0.0 0.0 0.011890606420927468 43 0.19817677368212447 0.0 0.0 0.0 0.011890606420927468 44 0.20214030915576695 0.0 0.0 0.0 0.011890606420927468 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTCTG 15 0.008035555 50.464787 41 CCTTTTC 20 4.040234E-4 50.162003 43 GAGCACA 15 0.008229576 50.162 49 CACAGGA 15 0.008229576 50.162 44 AGGAGCA 15 0.008229576 50.162 47 TAGTAGC 15 0.008295018 50.061874 33 ATCACAG 15 0.008295018 50.061874 42 AGCACAG 15 0.008295018 50.061874 50 TCTCTGG 15 0.008295018 50.061874 42 AAGAGTG 20 4.1209243E-4 49.962154 10 ACAACGA 20 4.1209243E-4 49.962154 12 ACAGTGT 15 0.008360846 49.96215 10 ACAGTAG 15 0.008360846 49.96215 29 AGTAGTA 15 0.008360846 49.96215 31 AACTGGT 15 0.008360846 49.96215 10 CAGTAGT 15 0.008360846 49.96215 30 GAAGTGT 15 0.008360846 49.96215 9 GAACGCA 15 0.008360846 49.96215 9 AACCTGT 15 0.008360846 49.96215 10 ACCTGTC 15 0.008360846 49.96215 11 >>END_MODULE