FastQCFastQC Report
Fri 17 Jun 2016
SRR1526221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16333
Sequences flagged as poor quality0
Sequence length56
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1630.9979795506030736No Hit
AACGCCGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1560.955121533092512No Hit
AACGCCGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG920.5632768015673789No Hit
AACGCCGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG900.5510316537072185No Hit
AACGCCGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG780.477560766546256No Hit
AACGCCGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG750.4591930447560154No Hit
AACGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.36735443580481236No Hit
AACGCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.26939325292352906No Hit
AACGCCGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG420.25714810506336866No Hit
AACGCCGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG310.18979979183248638No Hit
AACGCCGGGTACGCTCGACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.14694177432192493No Hit
AACGCCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG230.14081920039184473No Hit
AACGCCGGGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.12857405253168433No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT200.12245147860160412No Hit
AACGCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.11632890467152392No Hit
AACGCCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATC180.11020633074144372No Hit
AACGCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.10408375681136349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACA1100.050.69062839
CCCACAG308.9514106E-750.69062840
CCGCACA150.00784374550.69062840
CCACAGT308.9514106E-750.69062841
ACCCACA308.9514106E-750.69062839
TACGCAC1050.050.69062438
TACCCAC354.3652108E-850.6906238
GTACCCA402.1937012E-950.53270737
CAGCGCT150.00804025350.3757836
ATACAAA150.00804025350.3757836
GGTACCC402.2555469E-950.37577436
TGATGCT150.00813986950.21981413
CAGTCTA150.00813986950.21981414
GATATGA150.00813986950.2198149
ATGATGC150.00813986950.21981412
CCAAAAC251.9454767E-550.21981443
GATGCTA150.00813986950.21981414
AACCTGC150.00813986950.21981442
ACCTGCG150.00813986950.21981443
GCAGTGG251.9454767E-550.21981412