##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526221_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16333 Sequences flagged as poor quality 0 Sequence length 56 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.49996938713035 38.0 38.0 39.0 34.0 40.0 2 37.27025041327374 39.0 38.0 39.0 34.0 40.0 3 36.078062817608526 39.0 35.0 39.0 29.0 40.0 4 35.45876446458091 38.0 35.0 39.0 28.0 40.0 5 36.20375926039307 39.0 36.0 39.0 30.0 40.0 6 36.63791097777506 39.0 38.0 39.0 32.0 40.0 7 36.52060246127472 38.0 36.0 39.0 32.0 40.0 8 36.725218882018 39.0 37.0 39.0 32.0 40.0 9 36.63240066123799 39.0 36.0 39.0 32.0 40.0 10 35.97153003122513 38.0 35.0 39.0 30.0 40.0 11 35.6489316108492 38.0 35.0 39.0 29.0 40.0 12 33.41272270862671 38.0 32.0 39.0 19.0 40.0 13 33.0535725218882 37.0 31.0 39.0 18.0 40.0 14 33.1830649605094 37.0 31.0 39.0 19.0 40.0 15 33.12594134574175 37.0 31.0 39.0 18.0 40.0 16 32.1143696810139 36.0 29.0 39.0 16.0 40.0 17 32.589787546684626 36.0 30.0 39.0 19.0 40.0 18 32.00293883548644 36.0 29.0 39.0 17.0 40.0 19 32.97324435192555 36.0 30.0 39.0 22.0 40.0 20 32.579930202657195 36.0 29.0 39.0 20.0 40.0 21 33.78062817608522 37.0 33.0 39.0 23.0 40.0 22 33.58329761831874 37.0 32.0 39.0 22.0 40.0 23 33.83811914528868 37.0 32.0 39.0 23.0 40.0 24 33.62793118226902 37.0 32.0 39.0 23.0 40.0 25 33.67721790240617 37.0 32.0 39.0 23.0 40.0 26 34.53297006061348 38.0 33.0 39.0 26.0 40.0 27 34.54282740464091 38.0 33.0 39.0 26.0 40.0 28 34.521765750321435 38.0 33.0 39.0 26.0 40.0 29 34.634849690810015 38.0 34.0 39.0 27.0 40.0 30 34.20902467397294 38.0 33.0 39.0 24.0 40.0 31 35.10157350150003 38.0 35.0 39.0 28.0 40.0 32 34.69429988367109 38.0 34.0 39.0 27.0 40.0 33 35.060858384865 38.0 35.0 39.0 28.0 40.0 34 35.024061715545216 38.0 35.0 39.0 28.0 40.0 35 35.10163472723933 38.0 35.0 39.0 28.0 40.0 36 33.91171248392824 36.0 33.0 39.0 27.0 40.0 37 33.704524582134326 35.0 32.0 39.0 26.0 40.0 38 32.98750994918264 35.0 32.0 39.0 24.0 39.0 39 33.56241964121717 35.0 32.0 39.0 26.0 40.0 40 29.612624747443824 35.0 24.0 39.0 7.0 39.0 41 25.4427233208841 31.0 13.0 37.0 5.0 39.0 42 30.838853854160288 35.0 29.0 38.0 10.0 39.0 43 29.2215759505296 34.0 27.0 38.0 4.0 39.0 44 29.185024184167023 34.0 27.0 38.0 4.0 39.0 45 30.45111124716831 35.0 29.0 38.0 4.0 39.0 46 30.460601236759935 35.0 29.0 38.0 4.0 39.0 47 29.752892916181963 35.0 28.0 38.0 4.0 39.0 48 30.249249984693567 35.0 29.0 38.0 4.0 39.0 49 27.258984877242394 35.0 15.0 38.0 4.0 39.0 50 29.96791771260638 35.0 28.0 38.0 4.0 39.0 51 30.060552256168492 35.0 29.0 39.0 4.0 40.0 52 29.863038021184106 35.0 29.0 39.0 4.0 40.0 53 29.573991305945018 35.0 28.0 39.0 4.0 40.0 54 28.451601053082715 35.0 22.0 39.0 4.0 40.0 55 29.021673911712483 35.0 26.0 39.0 4.0 40.0 56 28.950713279862853 35.0 23.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.7796413089481362 1 2 1.668076318610801 1 3 3.024995145704665 1 4 3.4307144365804945 1 5 3.1874355482384047 1 6 2.6969424488380582 1 7 2.1796290811830943 1 8 1.979556379947418 1 9 2.3445807288147194 1 10 2.662573142611585 1 11 -3.353280826157704 1 12 -11.537259708142042 1 13 -9.707561525118045 1 14 -4.761400702489105 1 15 -8.532384836376792 1 16 -22.65926006551085 1 17 -4.404043094755185 1 18 -12.806851018936488 1 19 -5.661126564772786 1 20 -5.256540457045222 1 21 -5.310055127353227 1 22 -1.989159086310643 1 23 -3.9247951161456562 1 24 -4.643533148823742 1 25 -3.1046632063850055 1 26 3.116247155179096 1 27 3.478311560095797 1 28 2.034769789684553 1 29 2.564726008036594 1 30 3.2446384989434236 1 31 2.799939912587689 1 32 3.608477933505263 1 33 2.0036087246994114 1 34 2.0220372625550453 1 35 2.730562632639085 1 36 3.3882660580870265 1 37 3.8475417010594057 1 38 2.369705125964842 1 39 1.9970787910758716 1 40 5.514120869253812 1 41 -11.76491324495929 1 42 -14.386950245210754 1 43 -18.450768772777664 1 44 -22.745361264268134 1 45 -14.658047246629533 1 46 -17.79599030693971 1 47 -24.185364979271913 1 48 -15.983486062146495 1 49 -20.54124022178989 1 50 -15.80288538191215 1 51 -12.132758149687298 1 52 -12.159963220372376 1 53 -12.214430717108751 1 54 -11.301061874296451 1 55 -11.972149995209339 1 56 -8.181913484154649 2 1 1.345117499424326 2 2 1.668076318610801 2 3 3.3583284790380006 2 4 3.4009525318185894 2 5 3.1874355482384047 2 6 2.8219424488380582 2 7 2.5546290811830943 2 8 2.3188420942331334 2 9 2.523152157386143 2 10 2.924477904516344 2 11 1.8669572690803875 2 12 -2.1444025652848993 2 13 -0.07660914416566555 2 14 3.95288501179661 2 15 3.152138973147018 2 16 3.2990732678224823 2 17 3.3459569052448153 2 18 -0.4020891141745828 2 19 3.2555401018938817 2 20 3.380364304859537 2 21 3.3982782059801053 2 22 4.278698056546503 2 23 3.176395360044822 2 24 2.6838478035572066 2 25 3.2524796507578557 2 26 3.4317233456552856 2 27 2.1568829886672205 2 28 3.0942935992083633 2 29 2.9754402937508786 2 30 3.018448022752949 2 31 2.4844637221114994 2 32 3.2275255525528834 2 33 1.2833706294613165 2 34 2.2660848816026657 2 35 2.8019912040676545 2 36 3.6561232009441724 2 37 1.9249226534403547 2 38 2.161371792631506 2 39 2.568507362504441 2 40 5.752216107349053 2 41 -1.622056102102146 2 42 -5.214331197591704 2 43 -11.873387820396708 2 44 -14.358456502363373 2 45 -6.788999627581912 2 46 -10.331704592653995 2 47 -18.73298402689096 2 48 -9.775152728813161 2 49 -13.386478317027985 2 50 -9.273123477150246 2 51 -6.567281959211108 2 52 -5.904010839419996 2 53 -7.077525955203988 2 54 -6.045109493344071 2 55 -6.859054757114102 2 56 -5.175961103202269 3 1 1.3775249068317308 3 2 2.112520763055244 3 3 3.4694395901491077 3 4 3.451878457744513 3 5 3.298546659349512 3 6 3.141386893282501 3 7 2.624073525627537 3 8 2.7679161683072095 3 9 3.0694484536824405 3 10 2.5355890156274583 3 11 3.371586898710021 3 12 4.9759678050854745 3 13 2.414131596575075 3 14 4.434366493278091 3 15 4.0919537879618275 3 16 4.859258453007673 3 17 5.855216164504075 3 18 6.171984959899493 3 19 4.894428990782767 3 20 6.060919860415094 3 21 5.356611539313441 3 22 4.449994352842793 3 23 4.995839804489265 3 24 5.568107062816466 3 25 4.974701872980077 3 26 2.936352975284912 3 27 2.8698459516301824 3 28 2.8813306362454014 3 29 2.961551404861993 3 30 3.286966541271468 3 31 0.942797055444828 3 32 3.7830811081084335 3 33 3.056518777609469 3 34 2.30775154826933 3 35 2.524213426289876 3 36 4.197789867610837 3 37 4.355478208995912 3 38 4.814149570409285 3 39 4.8277666217637005 3 40 8.604067959200908 3 41 10.56312908308304 3 42 6.068076209815704 3 43 -0.720610042618933 3 44 4.386913868006996 3 45 6.0721114835291985 3 46 3.807184296234894 3 47 -3.12187291577985 3 48 4.423921345260915 3 49 7.141299460749796 3 50 3.509283930257162 3 51 3.205866188937037 3 52 1.072841012431855 3 53 2.1400666373886033 3 54 3.3021127288781535 3 55 2.8215007984414555 3 56 3.4953351930940286 4 1 1.970117499424326 4 2 2.562015712550192 4 3 3.979540600250118 4 4 4.435043440909496 4 5 3.535920396723249 4 6 3.772700024595629 4 7 3.270538172092188 4 8 3.345357245748289 4 9 3.333758217992205 4 10 3.1669021469405862 4 11 3.8480178751409966 4 12 6.560142889260554 4 13 5.764299946743428 4 14 5.816521375432977 4 15 4.307442003450049 4 16 7.189224782973998 4 17 7.277775087062995 4 18 7.143365431279964 4 19 6.414631010984788 4 20 6.929606729101966 4 21 5.674793357495261 4 22 5.9567283595768 4 23 4.263516572166033 4 24 5.733090227799636 4 25 5.555509953788153 4 26 3.215814254746192 4 27 3.0129435947278296 4 28 1.541263296178066 4 29 3.729228172538761 4 30 3.7987510530559803 4 31 3.367039479687257 4 32 4.9396467646740945 4 33 3.787158508249199 4 34 3.30775154826933 4 35 3.9383548404312876 4 36 3.1674868373078056 4 37 2.6635590170767216 4 38 4.460614216873935 4 39 3.99274978674687 4 40 7.070397925530873 4 41 10.30218632214028 4 42 6.266729408468901 4 43 8.06221824020935 4 44 6.955937437030567 4 45 5.9761518875696 4 46 6.978901467952063 4 47 6.5851977912908595 4 48 7.168029089368659 4 49 10.075642895093228 4 50 5.359452280425515 4 51 6.557718040788892 4 52 5.857352796943637 4 53 7.638383135705105 4 54 6.51928444604987 4 55 5.8871573640980195 4 56 7.153584351343184 5 1 1.3510698803767056 5 2 1.572838223372706 5 3 3.12023324094276 5 4 1.4783334841995384 5 5 3.1874355482384047 5 6 2.839799591695197 5 7 2.0367719383259484 5 8 1.979556379947418 5 9 2.2017235859575734 5 10 1.9006683807068256 5 11 2.456242983366103 5 12 5.462740291857955 5 13 5.6733908558343344 5 14 5.95288501179661 5 15 5.658091354099398 5 16 5.769311363060577 5 17 5.310242619530531 5 18 6.336006123920658 5 19 4.957921054274831 5 20 5.695840495335727 5 21 3.7851829678848716 5 22 5.201317104165547 5 23 4.599014407663866 5 24 4.594562089271491 5 25 4.847717745995951 5 26 3.6400566789886213 5 27 3.240216322000556 5 28 2.7490555039702684 5 29 3.7552021985127837 5 30 3.911305165610088 5 31 2.895178007825784 5 32 3.0370493620766936 5 33 2.8131325342232216 5 34 2.402989643507425 5 35 2.3019912040676545 5 36 4.340647010467983 5 37 3.2761131296308292 5 38 4.083990840250557 5 39 3.9494597434568277 5 40 8.847454202587148 5 41 11.044610564564522 5 42 5.660668802408292 5 43 7.4063740843651935 5 44 7.016543497636629 5 45 6.6752860867037995 5 46 6.4421049311555265 5 47 6.433682639775704 5 48 6.492704414043985 5 49 10.79209311154344 5 50 8.006638427611662 5 51 7.533908516979366 5 52 6.982893922484763 5 53 8.214140711462676 5 54 8.746557173322596 5 55 7.551659528600187 5 56 9.199038896797731 6 1 -0.6088298689967289 6 2 1.0189535115932529 6 3 2.972363566757295 6 4 3.315426216029117 6 5 2.2225232675366513 6 6 2.626767010241565 6 7 0.811208028551512 6 8 1.606122795987524 6 9 2.0538539117721086 6 10 0.7578112378496797 6 11 2.3910800760979356 6 12 4.921386908399313 6 13 4.883917171623807 6 14 5.479200801270295 6 15 6.101700376655785 6 16 5.112669759050558 6 17 4.859114799981658 6 18 4.203174043720157 6 19 4.268697996630724 6 20 4.154487111877085 6 21 3.7250325919450233 6 22 3.451943670581585 6 23 2.5864830793430684 6 24 1.7474442947852822 6 25 3.1033568437403076 6 26 3.2014601877605458 6 27 2.819163690421611 6 28 2.598679564120644 6 29 2.920615732347372 6 30 3.3298515315248736 6 31 1.0129724940413212 6 32 1.5257711665879654 6 33 0.1890723838472823 6 34 1.834067337743015 6 35 2.1967280461729146 6 36 3.1100705693652273 6 37 2.9578173902824574 6 38 3.1591788101753693 6 39 0.7965775379430369 6 40 7.752216107349053 6 41 4.711277231231186 6 42 2.9940021357416278 6 43 4.571787618199782 6 44 4.525315427461191 6 45 1.7153863373303686 6 46 2.8832076880477544 6 47 1.661752815214303 6 48 1.3498472711868388 6 49 6.398609402270264 6 50 0.7159116105690515 6 51 -1.0475451171058445 6 52 -0.5684845236305236 6 53 -0.8735785867829371 6 54 -0.5692323003616124 6 55 -2.4358091430790125 6 56 -0.3799084716233203 7 1 1.0610265903334195 7 2 2.1074702580047386 7 3 3.2977224184319383 7 4 3.116861622727683 7 5 2.990465851268702 7 6 3.1817909336865426 7 7 2.588719990274001 7 8 2.9362663366573756 7 9 2.333758217992205 7 10 1.9396294196678596 7 11 3.575290602413723 7 12 5.696506525624194 7 13 4.991572674016155 7 14 4.680157739069337 7 15 4.807442003450049 7 16 5.825588419337638 7 17 5.595956905244815 7 18 6.643365431279964 7 19 5.323721920075702 7 20 4.111424910920146 7 21 4.902066084767988 7 22 4.502182905031347 7 23 4.172607481256939 7 24 3.051272045981449 7 25 2.6918735901517934 7 26 3.397632436564379 7 27 1.9674890492732828 7 28 2.1321723870871523 7 29 1.911046354356941 7 30 3.2987510530559803 7 31 2.0943122069599838 7 32 2.1214649464922744 7 33 1.923522144612832 7 34 1.98956973008751 7 35 2.1201730222494746 7 36 3.9856686554896257 7 37 4.072649926167628 7 38 3.142432398692115 7 39 3.9472952412923235 7 40 9.024943380076326 7 41 -1.6523591324051772 7 42 6.084911226650721 7 43 6.925854603845718 7 44 6.637755618848747 7 45 6.567060978478693 7 46 6.70617419522479 7 47 6.676106882199946 7 48 7.304392725732292 7 49 10.484733804184135 7 50 8.086725007698242 7 51 8.239536222607072 7 52 8.175534615125457 7 53 7.4111104084323784 7 54 6.792011718777143 7 55 6.614430091370746 7 56 6.744493442252278 8 1 1.0135957602938888 8 2 1.8564821157122466 8 3 3.242386450052493 8 4 3.9172568796446754 8 5 3.245406562731155 8 6 2.9288265068090737 8 7 2.440498646400485 8 8 1.853262384088211 8 9 2.584746360284697 8 10 1.8447677595888123 8 11 2.8144210371963325 8 12 5.0403800434107495 8 13 4.369043029747381 8 14 5.387667620492259 8 15 4.422066509378901 8 16 6.83349355767756 8 17 5.378565600896987 8 18 5.8370413206080265 8 19 4.918583580154749 8 20 5.75174111645374 8 21 4.79139414800909 8 22 4.994277766691425 8 23 4.5224098527984395 8 24 5.207398528194894 8 25 4.636537621772348 8 26 3.1762885630465902 8 27 4.066303278522298 8 28 2.6558878021069177 8 29 3.5750779749103003 8 30 4.13076686333266 8 31 3.3630869105172962 8 32 2.807235697480415 8 33 3.2417039627946522 8 34 2.916447200443244 8 35 3.562860769285045 8 36 3.8789492879006957 8 37 3.4955748273534013 8 38 3.0218790390083186 8 39 3.5540146088812534 8 40 7.839172629088186 8 41 -8.201766247029685 8 42 2.907045614002495 8 43 4.9653802955453195 8 44 5.509297120825035 8 45 3.511725010099248 8 46 5.6864113493749855 8 47 5.506146407891649 8 48 5.045499445099885 8 49 9.073666610508244 8 50 5.681586667777289 8 51 5.4925006494845405 8 52 4.742728291014785 8 53 5.330082740448184 8 54 5.833513695061729 8 55 5.976090170422129 8 56 6.242517157667294 9 1 1.7428447721515994 9 2 2.2438338943683718 9 3 3.7977224184319383 9 4 3.4804979863640426 9 5 3.5813749421777956 9 6 3.4090636609592693 9 7 2.3159927175467274 9 8 2.663539063930102 9 9 2.7883036725376584 9 10 1.9396294196678596 9 11 1.8480178751409966 9 12 5.196506525624194 9 13 3.900663583107061 9 14 5.316521375432977 9 15 5.171078367086409 9 16 5.461952055701271 9 17 5.641411450699362 9 18 5.05245634037087 9 19 4.232812829166608 9 20 5.656879456374693 9 21 3.0838842665861677 9 22 4.184001086849534 9 23 3.4453347539842127 9 24 4.551272045981449 9 25 3.6009644992427 9 26 1.897632436564379 9 27 1.3311254129096497 9 28 0.5867178416326055 9 29 1.6837736270842143 9 30 2.4351146894196134 9 31 1.6852212978690702 9 32 3.0760104010377276 9 33 0.9235221446128321 9 34 2.0350242755420567 9 35 2.574718476794928 9 36 3.4856686554896257 9 37 3.4817408352585346 9 38 4.551523307783022 9 39 3.5836588776559566 9 40 6.206761561894506 9 41 4.711277231231186 9 42 4.175820317559808 9 43 5.289490967482077 9 44 5.22866470975784 9 45 5.430697342115053 9 46 6.569810558861157 9 47 7.676106882199946 9 48 5.577119998459565 9 49 7.848370167820502 9 50 4.995815916789148 9 51 4.784990768061618 9 52 4.9482618878527305 9 53 5.274746772068738 9 54 4.70110262786805 9 55 5.20533918227984 9 56 4.017220714979548 10 1 0.3385385520559083 10 2 1.5452693010669378 10 3 2.7618372509678224 10 4 3.3680577949764867 10 5 2.4856811622734867 10 6 2.2583459576099827 10 7 2.916471186446252 10 8 2.553491217040154 10 9 2.843327595982636 10 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115 25 4.019146317424521 115 26 1.3067233456552856 115 27 2.1068829886672233 115 28 -0.15570640079163667 115 29 0.8504402937508786 115 30 0.7017813560862791 115 31 -0.05720294455517205 115 32 1.0275255525528806 115 33 -0.6916293705386778 115 34 -0.69224845173067 115 35 0.10199120406765161 115 36 2.1977898676108367 115 37 0.7999226534403547 115 38 1.6363717926315076 115 39 2.501840695837778 115 40 4.818882774015723 115 41 5.111277231231185 115 42 -0.20599786425837152 115 43 -3.7650544870633773 115 44 -5.7834565023633715 115 45 1.0943337057514206 115 46 -8.281704592653998 115 47 -7.699650693557626 115 48 -7.450152728813162 115 49 -14.436478317027985 115 50 -6.83145681048358 115 51 -9.075615292544441 115 52 -8.979010839419995 115 53 -8.652525955203988 115 54 -15.253442826677404 115 55 -8.534054757114099 115 56 -7.467627769868937 116 1 0.922498451805275 116 2 1.3347429852774653 116 3 2.882138002847519 116 4 3.2878572937233486 116 5 2.3779117387145945 116 6 1.6017043535999633 116 7 1.9891528907069045 116 8 2.027175427566469 116 9 1.2017235859575734 116 10 1.8054302854687307 116 11 -2.4485189213958023 116 12 -15.537259708142042 116 13 -6.4218472394037605 116 14 -3.380448321536722 116 15 -7.722861026852986 116 16 -5.040212446463229 116 17 -1.4516621423742322 116 18 -6.902089114174583 116 19 -3.0896979933442132 116 20 -3.8279690284737953 116 21 3.1661353488372512 116 22 2.8203647232131672 116 23 2.551395360044822 116 24 3.3564668511762576 116 25 2.8953367936149945 116 26 2.878151917083855 116 27 3.0021210839053154 116 28 1.5109602658750347 116 29 3.421868865179455 116 30 2.720828975133898 116 31 1.990416103063879 116 32 2.8941922192195477 116 33 0.9083706294613165 116 34 2.164894405412184 116 35 1.9686578707343187 116 36 3.1977898676108367 116 37 3.3237321772498802 116 38 1.8935146497743673 116 39 3.3304121244092073 116 40 8.561739916872863 116 41 0.8541343740883285 116 42 -5.386950245210752 116 43 -9.784102106110996 116 44 -10.221551740458612 116 45 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28 2.8699346248493924 117 29 3.029927473238061 117 30 3.0504993048042266 117 31 2.2761303887781636 117 32 2.8941922192195477 117 33 2.2417039627946522 117 34 2.1539053944231767 117 35 1.9173758194522676 117 36 3.300353970174939 117 37 3.415307268824968 117 38 3.2927820490417616 117 39 2.978763772760857 117 40 8.13683149196444 117 41 6.095892615846569 117 42 -0.31369017195067883 117 43 -5.857362179371069 117 44 -8.624482143389013 117 45 0.3712567826744966 117 46 -3.82529433624374 117 47 -5.540676334583267 117 48 -4.265537344197778 117 49 -10.66211934266901 117 50 -4.000687579714349 117 51 -6.711512728441878 117 52 -6.009780070189226 117 53 -5.760218262896295 117 54 -5.638058211292787 117 55 -5.452003475062817 117 56 -3.7240380262791923 118 1 0.6844032137100413 118 2 1.4061715567060347 118 3 3.024995145704665 118 4 3.2402382461043047 118 5 2.0683879291907843 118 6 1.935037686933292 118 7 1.2510576526116637 118 8 2.027175427566469 118 9 2.177914062148048 118 10 2.0435255235639644 118 11 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49 -15.755525936075605 118 50 -18.37431395334072 118 51 -19.156567673496824 118 52 -18.99329655370571 118 53 -20.666811669489704 118 54 -21.253442826677404 118 55 -20.35310237616172 118 56 -19.658103960345127 119 1 1.036784166090996 119 2 0.9347429852774667 119 3 2.891661812371332 119 4 3.059285865151921 119 5 2.720768881571736 119 6 2.3636091155047225 119 7 1.3796290811830971 119 8 1.6938420942331334 119 9 1.4398188240528143 119 10 0.8244779045163497 119 11 -7.05804273091961 119 12 -8.794402565284898 119 13 -10.726609144165664 119 14 -5.580448321536725 119 15 -9.856194360186318 119 16 -15.525926732177515 119 17 -15.604043094755184 119 18 -15.235422447507917 119 19 -9.994459898106118 119 20 -8.094635695140461 119 21 0.023278205980105326 119 22 1.5536980565465015 119 23 0.6180620267114847 119 24 1.4421811368905395 119 25 0.5524796507578529 119 26 3.3067233456552856 119 27 3.173549655333886 119 28 1.9109602658750333 119 29 0.45044029375088 119 30 2.1017813560862777 119 31 0.4094637221114965 119 32 -5.505807780780451 119 33 -1.424962703872012 119 34 -2.825581785064003 119 35 -1.0980087959323441 119 36 -0.9355434657224961 119 37 0.46658932010701903 119 38 0.30303845929817186 119 39 0.7018406958377739 119 40 4.685549440682383 119 41 -1.622056102102146 119 42 -6.472664530925037 119 43 -19.031721153730043 119 44 -16.25012316903004 119 45 -18.23899962758191 119 46 -22.348371259320665 119 47 -23.23298402689096 119 48 -20.583486062146495 119 49 -20.169811650361318 119 50 -20.298123477150245 119 51 -20.142281959211108 119 52 -19.579010839419997 119 53 -19.58585928853732 119 54 -21.92010949334407 119 55 -18.66738809044743 119 56 -18.00096110320227 120 1 -0.404882500575674 120 2 0.8347429852774653 120 3 1.3999951457046649 120 4 3.0259525318185894 120 5 0.979102214905069 120 6 1.6136091155047225 120 7 0.9296290811830943 120 8 1.277175427566469 120 9 0.9814854907194785 120 10 -0.7421887621503203 120 11 -2.674709397586277 120 12 -14.8944025652849 120 13 -5.8266091441656656 120 14 -9.92211498820339 120 15 -13.306194360186318 120 16 -14.867593398844182 120 17 -7.279043094755185 120 18 -11.152089114174583 120 19 -6.369459898106118 120 20 -9.911302361807127 120 21 -8.643388460686559 120 22 -5.679635276786833 120 23 -5.156937973288514 120 24 -10.982818863109458 120 25 -3.83085368257548 120 26 2.9317233456552856 120 27 2.490216322000556 120 28 0.927626932541699 120 29 0.4337736270842143 120 30 1.5601146894196134 120 31 0.02613038877816365 120 32 -16.730807780780452 120 33 -1.1332960372053478 120 34 0.057751548269330044 120 35 0.30199120406765445 120 36 0.2811232009441724 120 37 0.42492265344035474 120 38 -0.5052948740351582 120 39 -1.0981593041622233 120 40 5.752216107349053 120 41 -4.788722768768814 120 42 -11.880997864258372 120 43 -12.790054487063376 120 44 -13.066789835696706 120 45 -16.08066629424858 120 46 -16.498371259320663 120 47 -18.60798402689096 120 48 -17.27515272881316 120 49 -20.09481165036132 120 50 -16.73145681048358 120 51 -15.442281959211108 120 52 -16.154010839419996 120 53 -18.07752595520399 120 54 -18.503442826677404 120 55 -16.692388090447434 120 56 -14.425961103202269 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 213.0 5 26.0 6 6.0 7 20.0 8 45.0 9 36.0 10 22.0 11 23.0 12 21.0 13 32.0 14 27.0 15 33.0 16 49.0 17 47.0 18 53.0 19 76.0 20 106.0 21 149.0 22 180.0 23 256.0 24 285.0 25 328.0 26 319.0 27 357.0 28 408.0 29 556.0 30 683.0 31 744.0 32 814.0 33 1009.0 34 1206.0 35 1423.0 36 1889.0 37 2126.0 38 1867.0 39 896.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.39998775485213 0.4347027490356946 0.08571603502112288 0.07959346109104268 8 98.7326271964734 1.0469601420437153 0.12857405253168433 0.09183860895120309 9 94.8998959162432 3.2939447743831507 0.9367538113022714 0.8694054980713892 10 16.714626829118963 59.939998775485215 13.120675932161882 10.224698463233944 11 21.257576685238476 37.04769485091533 29.35161942080451 12.343109043041695 12 49.63570685116023 15.612563521704526 14.957448111185942 19.794281515949304 13 22.160308615516502 27.95297287367583 18.65776743616435 31.22895107464332 14 33.04965407457295 24.08620584093553 20.63307414437029 22.231065940121226 15 25.596808837066586 30.622890457195457 21.859466093893833 21.92083461184412 16 22.039090741988847 20.53183015746584 35.16941363887017 22.259665461675144 17 35.40684503765383 24.588256903202108 18.637115043164147 21.367783015979917 18 31.75361253979917 26.445015919666915 19.43423952975753 22.367132010776388 19 20.101734387448673 39.04516761659619 18.949561806704665 21.903536189250474 20 20.79171517862614 25.38758502359213 19.1617133402782 34.658986457503524 21 20.026939325292354 25.62909447131574 30.9006306251148 23.443335578277107 22 23.008632829241414 24.08008326700545 26.602583726198493 26.308700177554645 23 22.29229167942203 37.959958366497276 22.163717626890346 17.584032327190354 24 20.49837751790853 34.99663258433846 25.09643053939876 19.408559358354253 25 19.837139533459865 34.666013592114126 28.745484601726567 16.751362272699446 26 24.064662298695733 22.570571306104952 37.94623721756169 15.418529177637621 27 28.46460312595771 19.66288691388293 35.9178669935642 15.95464296659516 28 14.025623735670937 32.73462882363759 39.73518053086495 13.504566909826519 29 12.02082057562768 30.434782608695656 44.133496631965706 13.410900183710961 30 9.221725552629968 20.07225522013349 59.80037964607189 10.905639581164655 31 8.385397525419576 18.314345216219525 63.022173220629675 10.278084037731226 32 20.200968077936402 16.37154586116047 54.83119906868452 8.596286992218614 33 7.440804809225862 26.855600539811064 57.12182554287818 8.581769108084899 34 6.008452256997612 16.187909597599067 60.825626263244935 16.978011882158388 35 17.150557393115275 15.502878843562415 60.97023153252481 6.376332230797501 36 17.391304347826086 15.523576240048989 60.685854255970604 6.399265156154317 37 16.108247422680414 15.175503190967108 62.45090819833088 6.2653411880216 38 6.085354620816703 16.377034080442122 71.74086582744857 5.7967454712926 39 5.820657507360157 25.95068694798822 61.98478900883219 6.24386653581943 40 4.964232856438086 14.109521460286137 54.40305870744944 26.523186975826345 41 12.214259236377115 12.411960953910787 50.25330532559001 25.12047448412208 42 6.228925265158482 17.644534363313102 61.12439458034456 15.002145791183864 43 5.109489051094891 23.333128871986748 59.43077961111452 12.12660246580384 44 5.11070110701107 16.629766297662975 58.16113161131611 20.09840098400984 45 5.749877270495827 27.08026509572901 60.80633284241531 6.3635247913598425 46 15.805937193326791 18.007850834151128 61.23037291462218 4.955839057899902 47 6.093826555609995 17.08721215090642 70.3454189122979 6.473542381185693 48 5.694146490959239 17.707631014403923 70.8979466748391 5.700275819797732 49 4.436104103857749 16.163169876330524 54.52531840275642 24.875407617055313 50 5.706754934412162 18.787544440357976 70.47321319112419 5.032487434105676 51 16.1333906528281 20.106859915249036 59.06159798562919 4.69815144629368 52 6.063021088769005 29.744973025993133 58.66233447768514 5.529671407552722 53 14.167433302667892 22.18337933149341 58.70591842992947 4.943268935909231 54 5.993129677340204 21.11397374555269 63.298981720034355 9.593914857072752 55 6.6912216083486795 21.233885819521177 57.94966236955187 14.125230202578267 56 16.29148009568791 22.400785131570878 55.90382138256763 5.403913390173588 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 2.0 12 7.0 13 32.0 14 52.0 15 68.5 16 85.0 17 138.5 18 192.0 19 234.5 20 277.0 21 338.5 22 438.0 23 476.0 24 540.0 25 604.0 26 842.5 27 1081.0 28 1167.5 29 1399.5 30 1545.0 31 1581.5 32 1618.0 33 1535.5 34 1453.0 35 1248.5 36 1044.0 37 833.0 38 483.5 39 345.0 40 277.0 41 209.0 42 207.0 43 205.0 44 197.0 45 189.0 46 189.0 47 249.0 48 309.0 49 499.5 50 690.0 51 773.0 52 856.0 53 731.0 54 493.0 55 380.0 56 368.0 57 356.0 58 315.5 59 275.0 60 237.0 61 199.0 62 193.5 63 181.5 64 175.0 65 161.5 66 148.0 67 131.5 68 115.0 69 89.0 70 63.0 71 54.0 72 34.5 73 24.0 74 22.0 75 20.0 76 16.0 77 12.0 78 10.0 79 6.5 80 5.0 81 3.5 82 2.0 83 2.0 84 2.0 85 1.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.012245147860160411 14 0.0 15 0.23265780934304783 16 0.07347088716096246 17 0.0 18 0.006122573930080206 19 0.09796118288128329 20 0.08571603502112288 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.012245147860160411 27 0.11020633074144372 28 0.12245147860160412 29 0.018367721790240616 30 0.012245147860160411 31 0.04285801751056144 32 0.07347088716096246 33 0.18979979183248638 34 0.03673544358048123 35 0.04285801751056144 36 0.018367721790240616 37 0.2265352354129676 38 0.29388354864384986 39 0.17755464397232595 40 0.7163411498193841 41 0.9000183677217901 42 0.13469662646176453 43 0.18367721790240618 44 0.446947896895855 45 0.2265352354129676 46 0.17755464397232595 47 0.03061286965040103 48 0.11020633074144372 49 0.48980591440641646 50 0.11632890467152392 51 0.30612869650401026 52 0.12857405253168433 53 0.17143207004224575 54 0.18979979183248638 55 0.26327067899344886 56 0.18367721790240618 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 16333.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.78515888079349 #Duplication Level Percentage of deduplicated Percentage of total 1 86.99528936742934 63.319659584889486 2 6.939771197846569 10.10224698463234 3 2.464670255720054 5.3817424845405 4 1.2449528936742935 3.6245637666074817 5 0.7065948855989233 2.571481050633686 6 0.49629878869448185 2.1673911712483926 7 0.2607671601615074 1.3285985428274047 8 0.19347240915208613 1.1265536031347578 9 0.18506056527590847 1.2122696381558808 >10 0.45423956931359355 4.793975387252801 >50 0.04205921938088829 2.4184167023816814 >100 0.016823687752355317 1.9531010836955858 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AACGCCGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 163 0.9979795506030736 No Hit AACGCCGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 156 0.955121533092512 No Hit AACGCCGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 92 0.5632768015673789 No Hit AACGCCGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 90 0.5510316537072185 No Hit AACGCCGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 78 0.477560766546256 No Hit AACGCCGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 75 0.4591930447560154 No Hit AACGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60 0.36735443580481236 No Hit AACGCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44 0.26939325292352906 No Hit AACGCCGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 42 0.25714810506336866 No Hit AACGCCGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 31 0.18979979183248638 No Hit AACGCCGGGTACGCTCGACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.14694177432192493 No Hit AACGCCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG 23 0.14081920039184473 No Hit AACGCCGGGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21 0.12857405253168433 No Hit AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 20 0.12245147860160412 No Hit AACGCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.11632890467152392 No Hit AACGCCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATC 18 0.11020633074144372 No Hit AACGCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.10408375681136349 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.006122573930080206 0.0 0.0 0.0 0.0 11 0.006122573930080206 0.0 0.0 0.0 0.0 12 0.006122573930080206 0.0 0.0 0.0 0.0 13 0.05510316537072185 0.0 0.0 0.0 0.0 14 0.05510316537072185 0.0 0.0 0.0 0.0 15 0.06734831323088226 0.0 0.0 0.0 0.0 16 0.06734831323088226 0.0 0.0 0.0 0.0 17 0.06734831323088226 0.0 0.0 0.0 0.0 18 0.07959346109104268 0.0 0.0 0.0 0.0 19 0.09183860895120309 0.0 0.0 0.0 0.0 20 0.09183860895120309 0.0 0.0 0.0 0.0 21 0.09183860895120309 0.0 0.0 0.0 0.0 22 0.09796118288128329 0.0 0.0 0.0 0.0 23 0.1040837568113635 0.0 0.0 0.0 0.0 24 0.12857405253168433 0.0 0.0 0.0 0.0 25 0.13469662646176453 0.0 0.0 0.0 0.0 26 0.15918692218208536 0.0 0.0 0.0 0.0 27 0.17143207004224575 0.0 0.0 0.0 0.0 28 0.17755464397232595 0.0 0.0 0.0 0.0 29 0.18367721790240618 0.0 0.0 0.0 0.0 30 0.18367721790240618 0.0 0.0 0.0 0.0 31 0.18979979183248638 0.0 0.0 0.0 0.0 32 0.208167513622727 0.0 0.0 0.0 0.0 33 0.208167513622727 0.0 0.0 0.0 0.0 34 0.208167513622727 0.0 0.0 0.0 0.0 35 0.208167513622727 0.0 0.0 0.0 0.0 36 0.208167513622727 0.0 0.0 0.0 0.0 37 0.2142900875528072 0.0 0.0 0.0 0.0 38 0.2204126614828874 0.0 0.0 0.0 0.0 39 0.2265352354129676 0.0 0.0 0.0 0.0 40 0.2326578093430478 0.0 0.0 0.0 0.0 41 0.23878038327312803 0.0 0.0 0.0 0.0 42 0.24490295720320823 0.0 0.0 0.0 0.0 43 0.24490295720320823 0.0 0.0 0.0 0.0 44 0.25102553113328846 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCACA 110 0.0 50.690628 39 CCCACAG 30 8.9514106E-7 50.690628 40 CCGCACA 15 0.007843745 50.690628 40 CCACAGT 30 8.9514106E-7 50.690628 41 ACCCACA 30 8.9514106E-7 50.690628 39 TACGCAC 105 0.0 50.690624 38 TACCCAC 35 4.3652108E-8 50.69062 38 GTACCCA 40 2.1937012E-9 50.532707 37 CAGCGCT 15 0.008040253 50.37578 36 ATACAAA 15 0.008040253 50.37578 36 GGTACCC 40 2.2555469E-9 50.375774 36 TGATGCT 15 0.008139869 50.219814 13 CAGTCTA 15 0.008139869 50.219814 14 GATATGA 15 0.008139869 50.219814 9 ATGATGC 15 0.008139869 50.219814 12 CCAAAAC 25 1.9454767E-5 50.219814 43 GATGCTA 15 0.008139869 50.219814 14 AACCTGC 15 0.008139869 50.219814 42 ACCTGCG 15 0.008139869 50.219814 43 GCAGTGG 25 1.9454767E-5 50.219814 12 >>END_MODULE