FastQCFastQC Report
Fri 17 Jun 2016
SRR1526220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56112
Sequences flagged as poor quality0
Sequence length56
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA1490.2655403478756772No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA1140.20316509837467922No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.1978186484174508No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.1924721984602224No Hit
AAAACGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCCAGGCTCACAACCAAA870.1550470487596236No Hit
AAAACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.1390076988879384No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC760.1354433989164528No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA600.10692899914456801No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA580.1033646991730824No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC580.1033646991730824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATG204.0238537E-450.31199336
AGCGTAG150.00818527950.31199336
TCGTGAT204.04182E-450.26666635
CAGCGTA150.0082145350.26666635
ACCCGGC150.00824385750.2214243
CGACTCC150.00824385750.2214244
CTCGTGA204.0961022E-450.13117634
TGGTAGG204.1143244E-450.08617450
TGTCTCG204.1326112E-450.04125631
GTCTTGC204.1326112E-450.04125615
AGATCGT150.0083619550.04125615
TTTGTCT204.150962E-449.99641429
GGTATCG204.169378E-449.95165616
AAGAGTG252.0846861E-549.9069810
AACCCCC204.1878584E-449.9069810
ATCCAGA150.00845134249.90697510
AGGCATT150.00845134249.90697510
AACAGTG150.00845134249.90697510
AAGCCTT150.00845134249.90697510
AAGCATT150.00845134249.90697510
Produced by FastQC (version 0.11.5)