##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526220_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 56112 Sequences flagged as poor quality 0 Sequence length 56 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.813676218990594 39.0 38.0 39.0 35.0 40.0 2 37.59320644425435 39.0 38.0 39.0 35.0 40.0 3 37.44537710293698 39.0 38.0 39.0 34.0 40.0 4 37.39873823781009 39.0 38.0 39.0 34.0 40.0 5 36.29560521813516 39.0 36.0 39.0 30.0 40.0 6 36.48802395209581 39.0 36.0 39.0 31.0 40.0 7 36.98508340461933 39.0 38.0 39.0 33.0 40.0 8 36.87562375249501 39.0 38.0 39.0 32.0 40.0 9 36.74255061305959 39.0 37.0 39.0 32.0 40.0 10 36.40319361277445 39.0 36.0 39.0 31.0 40.0 11 35.50080196749359 38.0 35.0 39.0 29.0 40.0 12 33.5046870544625 38.0 32.0 39.0 19.0 40.0 13 33.49663173652694 38.0 32.0 39.0 19.0 40.0 14 33.746489164528086 38.0 33.0 39.0 20.0 40.0 15 33.62562375249501 38.0 33.0 39.0 19.0 40.0 16 32.90788066723695 37.0 30.0 39.0 18.0 40.0 17 33.027801539777585 37.0 30.0 39.0 20.0 40.0 18 32.410714285714285 36.0 30.0 39.0 17.0 40.0 19 33.328396777872825 37.0 31.0 39.0 22.0 40.0 20 33.166470630168234 36.0 30.0 39.0 22.0 40.0 21 34.066028656971774 38.0 33.0 39.0 23.0 40.0 22 33.84443612774451 38.0 33.0 39.0 23.0 40.0 23 34.175702167094386 38.0 33.0 39.0 24.0 40.0 24 33.93600299401198 38.0 33.0 39.0 23.0 40.0 25 34.02070858283433 38.0 33.0 39.0 23.0 40.0 26 34.84571927573425 38.0 35.0 39.0 27.0 40.0 27 34.7346378671229 38.0 34.0 39.0 26.0 40.0 28 34.7206123467351 38.0 34.0 39.0 26.0 40.0 29 34.60495081266039 38.0 34.0 39.0 26.0 40.0 30 34.516734388366125 38.0 34.0 39.0 25.0 40.0 31 34.88057812945537 38.0 35.0 39.0 27.0 40.0 32 34.537870687197035 38.0 34.0 39.0 26.0 40.0 33 34.65488665526091 38.0 35.0 39.0 27.0 40.0 34 34.54589036213288 38.0 34.0 39.0 26.0 40.0 35 34.423973481608215 38.0 34.0 39.0 26.0 40.0 36 33.47139649272883 36.0 33.0 39.0 25.0 40.0 37 33.22009552323924 36.0 32.0 39.0 23.0 40.0 38 32.95879669232963 36.0 32.0 39.0 23.0 40.0 39 32.714089677787285 36.0 32.0 39.0 20.0 40.0 40 29.129205873966352 35.0 23.0 38.0 6.0 39.0 41 25.718438123752495 31.0 13.0 38.0 4.0 39.0 42 30.138900769888792 35.0 28.0 38.0 4.0 39.0 43 28.52065511833476 33.0 24.0 38.0 4.0 39.0 44 28.530118334759052 33.0 24.0 38.0 4.0 39.0 45 29.628831622469345 34.0 28.0 38.0 4.0 39.0 46 29.251158397490734 35.0 27.0 38.0 4.0 40.0 47 28.823816652409466 35.0 26.0 38.0 4.0 40.0 48 28.896920444824637 35.0 26.0 38.0 4.0 40.0 49 26.25566723695466 33.0 11.0 38.0 4.0 39.0 50 28.468491588252068 34.0 25.0 38.0 4.0 39.0 51 28.700990875392073 35.0 25.0 39.0 4.0 40.0 52 28.39344525235244 35.0 23.0 39.0 4.0 40.0 53 28.020637296834902 35.0 22.0 39.0 4.0 40.0 54 27.121988166524094 34.0 12.0 39.0 4.0 40.0 55 27.482517108639865 34.0 17.0 39.0 4.0 40.0 56 26.676771457085827 34.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.5885964256168776 1 2 1.1703935185788623 1 3 1.2325764715868957 1 4 1.5137123734700282 1 5 2.19525580642663 1 6 2.1981221373771618 1 7 1.7689075861382335 1 8 1.7200752897687508 1 9 1.9290776849822535 1 10 2.1611119153313254 1 11 -2.0282826451569846 1 12 -12.600993281599344 1 13 -7.349024793934447 1 14 -6.517969912257524 1 15 -6.355697491062465 1 16 -22.025127860130574 1 17 -3.916559445973128 1 18 -11.954242240566906 1 19 -5.426924872155507 1 20 -5.610656718271603 1 21 -5.226672109975091 1 22 -1.9846783519081406 1 23 -2.658441612097402 1 24 -3.355485374113929 1 25 -2.4674263143649746 1 26 2.881609792989316 1 27 2.892030056454672 1 28 2.9017204051556575 1 29 3.012665521436247 1 30 3.038487039601712 1 31 2.4777272228656955 1 32 2.6476734755611275 1 33 3.076364022091248 1 34 1.8839973021980825 1 35 2.9240773933570168 1 36 3.7251976044830286 1 37 4.004280997243185 1 38 3.9946526660265356 1 39 4.017505810198145 1 40 6.669189411711034 1 41 -9.088576342401947 1 42 -8.98456237300281 1 43 -15.831105304306499 1 44 -20.9213346838183 1 45 -10.577642343438 1 46 -14.65457537913258 1 47 -22.107933463287893 1 48 -13.97694808262046 1 49 -18.662507250348398 1 50 -13.311407985255347 1 51 -9.991474280284063 1 52 -9.334038891656146 1 53 -9.73836194686114 1 54 -9.018610458830427 1 55 -9.328294875226447 1 56 -5.350193359006866 2 1 1.3215256465118728 2 2 1.6216325986287856 2 3 1.8232343428651987 2 4 1.9804622397563563 2 5 2.429343879166865 2 6 2.2139894934121074 2 7 2.0263509806827145 2 8 1.6683726689807301 2 9 2.0474588580968884 2 10 1.4707927853616312 2 11 1.1284297813004969 2 12 -1.3250082575308824 2 13 -1.158259951716584 2 14 3.3524169659738874 2 15 2.866267208527482 2 16 2.657168449372012 2 17 3.843290794711489 2 18 -0.8167845832304828 2 19 2.685929469081792 2 20 2.881053033912387 2 21 2.3784268381440015 2 22 3.101611539338073 2 23 3.01030504506074 2 24 1.511692375930643 2 25 2.7623829207928097 2 26 2.4416026615933504 2 27 2.4732388280717146 2 28 2.011722188004647 2 29 2.5644572846738996 2 30 2.6325323239661245 2 31 2.130963093787429 2 32 2.2590123951546346 2 33 2.205798858960563 2 34 2.2263043087946244 2 35 2.850267445059643 2 36 2.8261068574693056 2 37 3.4132665561663487 2 38 3.5530409705371433 2 39 3.366765927866176 2 40 7.275358069225742 2 41 -6.407528027194246 2 42 -7.8749171599523535 2 43 -12.782433526806052 2 44 -14.830765954989632 2 45 -7.562309842100866 2 46 -10.759585184802042 2 47 -18.24699568471774 2 48 -8.344036690215397 2 49 -12.138527931396712 2 50 -7.8207679424669685 2 51 -1.6759100085778762 2 52 -1.1833881517738156 2 53 -1.5839672240941596 2 54 -1.0747702021001722 2 55 -1.1623116340069792 2 56 2.232085122005792 3 1 1.6655581962406245 3 2 1.9775699102622966 3 3 2.16651342665061 3 4 2.0871530173332076 3 5 3.0366374294983913 3 6 2.896478944888898 3 7 2.4063992025030885 3 8 2.6023690523442085 3 9 2.8957108171083448 3 10 2.915639078309191 3 11 3.441871613729674 3 12 5.302930259648143 3 13 3.8681113563002967 3 14 4.5890052721824475 3 15 3.1278705840549037 3 16 5.242310100969359 3 17 5.265533109358866 3 18 6.063866411344563 3 19 5.074416509467561 3 20 5.018786608354823 3 21 4.53439428841525 3 22 4.641243847957725 3 23 3.7966221035296925 3 24 4.588847288528591 3 25 4.284082739960979 3 26 3.4174917514064873 3 27 3.02432381903013 3 28 2.660607058408509 3 29 3.6104187072176046 3 30 3.29754136555745 3 31 3.404621924408282 3 32 3.4405976874994195 3 33 3.199771131413847 3 34 2.799993278053215 3 35 2.5880612967775463 3 36 4.3883431443891325 3 37 3.94777529637129 3 38 4.151594315925937 3 39 3.9843066150271227 3 40 8.70272395228783 3 41 8.839156722655058 3 42 4.449374582060905 3 43 -0.16594769196578696 3 44 2.1277934181267035 3 45 4.1809089644090776 3 46 3.004881361310076 3 47 -1.7787919475266598 3 48 3.106385250712872 3 49 6.041550429061395 3 50 4.140805294422723 3 51 4.565199093290719 3 52 4.957208593253313 3 53 4.8100954642723295 3 54 5.897050171618936 3 55 4.09055153657771 3 56 4.102187593374087 4 1 1.096621319164214 4 2 1.6525422247694976 4 3 1.5856717876694404 4 4 2.070541721652141 4 5 2.2597825014806716 4 6 2.851905301478048 4 7 2.21813785145104 4 8 2.226953769952182 4 9 2.2143708392567376 4 10 1.9837453906015483 4 11 3.510531635878067 4 12 5.0429634822395 4 13 4.784778022966961 4 14 4.710821440510244 4 15 3.8681806615742858 4 16 5.436385405509778 4 17 4.883473308694409 4 18 4.882803288420973 4 19 4.324139654539543 4 20 4.122884061289483 4 21 3.254493956189336 4 22 3.541852928799365 4 23 3.3088037203624765 4 24 3.6962670005994696 4 25 3.4501956967716154 4 26 3.2109579751052735 4 27 2.550071327335772 4 28 2.7292670805569017 4 29 3.255213834570867 4 30 2.8581725947933307 4 31 2.2656407505433904 4 32 1.9956918181749472 4 33 1.9184865245478449 4 34 2.101488294664513 4 35 1.6893902004320296 4 36 3.4600485707457267 4 37 3.211618042772173 4 38 3.7761790335339107 4 39 4.20191458844905 4 40 7.678360718622269 4 41 9.811194374925275 4 42 5.6941143384285695 4 43 7.239644777580171 4 44 7.147450118015964 4 45 5.102835874708084 4 46 4.997406278253596 4 47 4.1002224489296 4 48 5.174214708077216 4 49 7.758881547555305 4 50 5.149941507047309 4 51 3.7816996470005755 4 52 4.034451118170253 4 53 3.8851231497651284 4 54 4.377393471729679 4 55 3.371836143443712 4 56 4.649806640993134 5 1 1.1281017502475947 5 2 1.7208916291937726 5 3 1.76349368216745 5 4 1.9826234006084675 5 5 2.98553406132185 5 6 2.9188366684661347 5 7 1.9209172048259688 5 8 2.3131704460724265 5 9 2.6346771353429546 5 10 1.9295763605391585 5 11 3.1503501270862344 5 12 5.575578343271836 5 13 5.333558510771837 5 14 4.851490754180126 5 15 4.985594161290678 5 16 5.807060391329404 5 17 6.068668997860609 5 18 6.570835052459543 5 19 4.532023942684752 5 20 5.287660011374562 5 21 4.145021466115601 5 22 4.6845074948799095 5 23 4.527748700509953 5 24 4.439602224649384 5 25 4.085476468183977 5 26 2.707271077771182 5 27 3.448539846904694 5 28 2.869085571765069 5 29 3.726853085847104 5 30 3.380293978797866 5 31 2.8796509604129312 5 32 2.870620916303139 5 33 3.4459964508099006 5 34 2.617937528924294 5 35 3.689106592944789 5 36 4.203383794795641 5 37 4.4271597330727985 5 38 4.667273758434643 5 39 4.865839716072415 5 40 9.581934172961464 5 41 10.929458697103374 5 42 6.985379227821653 5 43 8.713552888754304 5 44 8.363275415803823 5 45 6.860351473119884 5 46 6.037111326466867 5 47 5.445656368385073 5 48 5.815734844208805 5 49 8.557520231616564 5 50 5.619028290938402 5 51 5.09423509793648 5 52 4.815222530535543 5 53 5.58506642826768 5 54 6.942055978819866 5 55 5.8687164610840945 5 56 4.564440787334597 6 1 1.4860826085371883 6 2 1.7735313328060016 6 3 2.063740671979126 6 4 1.9931204676044558 6 5 2.7837742589137022 6 6 2.922850252905775 6 7 2.19090794270803 6 8 2.6810308968288368 6 9 2.629737339109546 6 10 2.660666203083153 6 11 3.432227249654929 6 12 5.143346172848865 6 13 4.284778022966961 6 14 4.884062535594147 6 15 4.992849487008492 6 16 4.6445102215239125 6 17 5.716708516230472 6 18 5.866759720566989 6 19 4.5720054184488745 6 20 4.311432780747829 6 21 4.454376205232613 6 22 4.228193817819083 6 23 4.314102513415165 6 24 5.00536326200659 6 25 4.382636085349773 6 26 3.7960330413401806 6 27 3.5618464230084257 6 28 3.682608263954016 6 29 3.6530648796106107 6 30 3.189494349282583 6 31 2.763874486192492 6 32 3.8412908761672924 6 33 2.661495061492211 6 34 3.669342283478173 6 35 3.635077571641922 6 36 3.6995245789882887 6 37 4.9322538979385016 6 38 4.67962324901816 6 39 5.265500105081372 6 40 8.933585917327004 6 41 5.12411754242601 6 42 5.682044865364098 6 43 7.647946220029393 6 44 6.447715057668599 6 45 4.412373702202935 6 46 5.303705954438467 6 47 5.765662543130361 6 48 5.655963309784603 6 49 8.064003714172905 6 50 5.4956877537355595 6 51 5.830419105346177 6 52 6.082434633036314 6 53 6.023627712614704 6 54 6.431558911823878 6 55 5.9642706444740305 6 56 5.054869932132373 7 1 1.7653750460574713 7 2 2.1808666135590045 7 3 2.459553719690902 7 4 2.6164570466372368 7 5 3.6831575760185373 7 6 3.5439148423247957 7 7 2.8624431585470447 7 8 2.9723324223075736 7 9 2.9314251028226295 7 10 2.9852361479062637 7 11 3.6763038481619077 7 12 5.778204984922866 7 13 5.77195751014645 7 14 5.634792817969995 7 15 5.897101353285677 7 16 6.536266144925403 7 17 6.439525783351538 7 18 7.021741869220023 7 19 5.415075850126904 7 20 5.853951443519655 7 21 5.024321028341994 7 22 4.777690734404608 7 23 4.42299572990332 7 24 5.11815131783262 7 25 5.042324498202738 7 26 4.190385524300261 7 27 3.669928214634524 7 28 3.4591418569433046 7 29 3.9776348244337214 7 30 4.007248325264406 7 31 3.3515083712947273 7 32 3.9506709475726822 7 33 3.7840202156629346 7 34 3.636073864133799 7 35 3.9836656923819405 7 36 3.266548272594264 7 37 4.049423648019641 7 38 3.790788455120076 7 39 4.1747828055033125 7 40 7.5653613149251875 7 41 0.880365513863854 7 42 5.89626102894735 7 43 7.6078618318437385 7 44 7.690980231313514 7 45 5.028894312394428 7 46 6.375760482189197 7 47 6.403442484707774 7 48 6.2794621737897955 7 49 9.34832698583795 7 50 7.302893206510632 7 51 8.041389569481193 7 52 8.086329472374189 7 53 8.164491068667935 7 54 9.167196691765454 7 55 8.006302452328622 7 56 7.008780999967492 8 1 1.336407617090046 8 2 1.7311091638015483 8 3 1.9605932207304093 8 4 1.9404348706150572 8 5 2.7940376868986476 8 6 2.8446771430870967 8 7 2.5003502964541795 8 8 2.256809206784361 8 9 2.381875553273076 8 10 1.803669966340081 8 11 2.9888471607052196 8 12 5.016250722968607 8 13 5.068561806750743 8 14 4.732505915683092 8 15 4.799041755226078 8 16 4.68214279080205 8 17 5.539664383490138 8 18 5.845719693197843 8 19 4.015214450265503 8 20 4.576058165626378 8 21 3.6608421648631264 8 22 3.556623513337385 8 23 3.5907018976094918 8 24 4.180867880549187 8 25 3.3593723152505603 8 26 2.644647478562213 8 27 2.7666018113081208 8 28 3.0589339567354017 8 29 3.4312037780026756 8 30 3.296576114592199 8 31 2.523026042812404 8 32 2.688023684925419 8 33 2.3342627659054784 8 34 2.6156303436902846 8 35 2.215474424190674 8 36 2.552757558803549 8 37 2.7119323105283044 8 38 2.833061497393114 8 39 2.963071093791598 8 40 6.1010508506147225 8 41 -1.5929539594556203 8 42 3.7704814031677287 8 43 5.6123663363482414 8 44 5.554112594445879 8 45 3.0670093505094655 8 46 3.31858792501664 8 47 3.5728811541464403 8 48 3.3338627281903506 8 49 5.724626362137329 8 50 3.3985273021447284 8 51 3.631479659571287 8 52 3.9407993268440435 8 53 4.711961616138481 8 54 4.024785049353813 8 55 3.969573415599587 8 56 3.555212046398541 9 1 1.166061214349277 9 2 1.8746188706915419 9 3 1.8382102744037994 9 4 1.9925856129066446 9 5 2.36426989093367 9 6 2.8037559401940655 9 7 2.613978008673442 9 8 2.6778217686419907 9 9 2.7037255723061975 9 10 2.7808302251904777 9 11 2.718196228082455 9 12 5.7511193944569925 9 13 5.031256896206401 9 14 4.65104417224952 9 15 5.461203917388239 9 16 5.594590449728582 9 17 5.604567314590252 9 18 5.749983111545774 9 19 4.235046958830409 9 20 4.163991169052338 9 21 3.871919439320685 9 22 4.3956033382327035 9 23 4.31338937381809 9 24 4.630430118843819 9 25 3.9973624180293896 9 26 3.0083703563696034 9 27 3.7793540001166477 9 28 3.3101711093810096 9 29 3.618299324253151 9 30 4.094825067770728 9 31 2.914346941175552 9 32 3.3518988276738 9 33 3.5411527544856085 9 34 3.1656874430431543 9 35 3.8818238722302567 9 36 3.5420990129337326 9 37 3.793013391609385 9 38 3.4876104125054077 9 39 4.454125528508008 9 40 6.9367950455138505 9 41 4.207198305485377 9 42 5.691493965025359 9 43 7.9294580759751945 9 44 7.6983836260408545 9 45 5.295597093181719 9 46 5.345424620867416 9 47 4.849813015585347 9 48 4.02305191737954 9 49 7.168478665144562 9 50 3.6040849724911332 9 51 3.9803567056314293 9 52 4.299763925245262 9 53 4.726426785533228 9 54 4.840544470747037 9 55 3.953395265618621 9 56 3.086426359302994 10 1 1.471542752225183 10 2 1.6500280827204676 10 3 2.0011337612709497 10 4 2.2242186543988467 10 5 2.4426863355473003 10 6 2.8581906566006126 10 7 2.3246746207785094 10 8 2.5270794770546274 10 9 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115 35 1.3155745242907741 115 36 2.360565366611354 115 37 2.163383761979759 115 38 2.434663098994715 115 39 2.378486509207015 115 40 6.673623423187298 115 41 1.933072066570407 115 42 -2.2120010793147564 115 43 -7.16991594593404 115 44 -8.508449968116683 115 45 -2.768826485326368 115 46 -7.279510173081459 115 47 -7.184876603611315 115 48 -7.310281415953792 115 49 -13.705804068293103 115 50 -7.781152377534948 115 51 -7.403054874963249 115 52 -7.462103576058858 115 53 -7.797047392870528 115 54 -12.503743479174714 115 55 -7.369765923739752 115 56 -6.665008173821679 116 1 0.9997799446414746 116 2 1.4192898818330164 116 3 1.623158771355655 116 4 1.4922702881221213 116 5 2.3184426889155887 116 6 2.6887675021028272 116 7 1.990265584876937 116 8 2.377422357248257 116 9 2.4264499784445164 116 10 2.3591358800149465 116 11 -2.128135493590868 116 12 -12.898029228624779 116 13 -7.565968245689756 116 14 -2.956481579274552 116 15 -8.225363246790867 116 16 -3.658720460508963 116 17 -1.5287099610036101 116 18 -7.59498220272793 116 19 -2.7098763121386895 116 20 -3.557261721424851 116 21 3.4165904504621594 116 22 3.964449249521337 116 23 2.924909239279515 116 24 3.566103862800091 116 25 2.9717160022211075 116 26 2.2305692212003763 116 27 2.788183094083429 116 28 2.0682118913864755 116 29 2.560489780423005 116 30 3.1957289988197104 116 31 2.480670254187956 116 32 3.2350184408754004 116 33 2.582144976474261 116 34 3.0851745147269867 116 35 3.1765474375024922 116 36 4.173169015035903 116 37 4.076595481161625 116 38 4.232617770083717 116 39 4.0578673826177365 116 40 7.968537740489676 116 41 3.6591638299756006 116 42 -2.0268158941295695 116 43 -8.30009836716124 116 44 -6.556819232903859 116 45 -0.34787568377855393 116 46 -5.271771090715511 116 47 -4.070725138713584 116 48 -5.126201813963743 116 49 -8.546047296596033 116 50 -3.839471891078343 116 51 -2.759605455394425 116 52 -2.3407658203927433 116 53 -2.7995349550595847 116 54 -8.858635684813187 116 55 -1.970926786094644 116 56 -1.1263127619919402 117 1 0.3471058152882449 117 2 0.6888794340718221 117 3 0.8361562837934713 117 4 0.9978673030474923 117 5 1.9443755247364862 117 6 1.958978944888898 117 7 1.4986611072649936 117 8 1.6886785761537269 117 9 1.3689251028226295 117 10 1.740043840213957 117 11 -3.939080767222709 117 12 -10.125641168923288 117 13 -11.506888643699707 117 14 -10.40206615638898 117 15 -10.122129415945093 117 16 -11.954118470459214 117 17 -10.892204985879232 117 18 -12.784347874369722 117 19 -6.166654919103866 117 20 -5.838356248788035 117 21 0.686180002700965 117 22 1.4299343241481992 117 23 0.21626496067255374 117 24 0.2287282409095468 117 25 1.135273216151461 117 26 2.390706037120772 117 27 2.673133342839648 117 28 2.050488010789458 117 29 2.149109183408079 117 30 2.779684222700304 117 31 1.9700981148844718 117 32 2.0804786398803756 117 33 2.479533036175752 117 34 2.410112325672266 117 35 2.6952041539204004 117 36 2.9508431443891325 117 37 2.8346800582760494 117 38 2.8837371730687913 117 39 3.508116138836641 117 40 7.550938238002114 117 41 3.1576091036074416 117 42 -2.9226492274629017 117 43 -8.18380483482293 117 44 -8.297801819968534 117 45 -1.8012338927337765 117 46 -3.1707138767851646 117 47 -4.6270062332409445 117 48 -4.143614749287128 117 49 -10.571544809033844 117 50 -5.966337562720135 117 51 -7.208610430518803 117 52 -6.596362835318118 117 53 -6.505380726203864 117 54 -9.823187923619159 117 55 -6.376710368184195 117 56 -6.183526692340198 118 1 0.7225490776995898 118 2 1.150315604284586 118 3 1.3392591206728994 118 4 1.4579736860262145 118 5 2.163790418353507 118 6 2.2883229165201087 118 7 1.9388206817330769 118 8 1.8531289307636598 118 9 2.1619215567233354 118 10 2.0463381664550866 118 11 -2.031279348782995 118 12 -7.843726275306267 118 13 -9.140753891926657 118 14 -10.3537505535521 118 15 -8.666455657079847 118 16 -9.827345420813824 118 17 -9.577931936233842 118 18 -8.987805321178232 118 19 -4.25131803967124 118 20 -3.6220441920504456 118 21 1.956126811211604 118 22 2.5376470901056436 118 23 2.1821337549987945 118 24 2.016405545874086 118 25 2.309918606222382 118 26 2.4195180938583647 118 27 3.025083697449581 118 28 3.049158223555416 118 29 2.8685240770251 118 30 3.319577839721582 118 31 2.439069746090148 118 32 -4.222269587069981 118 33 2.2321926106438355 118 34 1.3068321838282912 118 35 2.274991387962956 118 36 3.961038179850128 118 37 3.9632261575668295 118 38 3.7746946198773053 118 39 3.894640961531678 118 40 8.20829639403048 118 41 5.200605557508151 118 42 -0.8778797239168021 118 43 -5.991428948298108 118 44 -6.71845784833733 118 45 -1.6762338927337765 118 46 -11.295270614373816 118 47 -10.938619708418251 118 48 -11.44503318900344 118 49 -11.687679560806894 118 50 -9.806763094635027 118 51 -7.986979224845044 118 52 -7.221362835318118 118 53 -8.219033208473366 118 54 -12.182230476810648 118 55 -8.186550793716108 118 56 -5.648065699432397 119 1 0.47678268603654317 119 2 0.8177059646840661 119 3 0.945424991276461 119 4 1.1483775071291262 119 5 1.9992224635119982 119 6 2.09205717618142 119 7 1.4846304950200988 119 8 1.6533894605074693 119 9 1.2817652388770497 119 10 1.548292139533686 119 11 -4.320033148175092 119 12 -10.008294230147776 119 13 -11.266242385196303 119 14 -11.979021938701905 119 15 -7.722469551999517 119 16 -14.300206905833363 119 17 -15.1120179110493 119 18 -15.34429685396156 119 19 -7.289529068763731 119 20 -8.69890046647511 119 21 0.03949632923157509 119 22 0.87763840578085 119 23 0.5755336681555434 119 24 0.5956500096170245 119 25 1.028980699144654 119 26 2.1385801867806364 119 27 1.4579972884178787 119 28 2.2558451536466038 119 29 2.0100785711631843 119 30 1.8332556512717346 119 31 0.49986001964637694 119 32 -2.486273060799899 119 33 0.9412677300533048 119 34 0.5908096045838249 119 35 1.139081704940807 119 36 3.165554028742875 119 37 3.5889317589563206 119 38 3.11077798939532 119 39 3.9536943701291634 119 40 7.083676333240209 119 41 -0.47546912768507354 119 42 -5.781917934945895 119 43 -14.388736807612046 119 44 -13.037172568267854 119 45 -9.227254300897041 119 46 -17.438996189710334 119 47 -16.266887185621897 119 48 -15.181029715273521 119 49 -15.603007394067857 119 50 -13.282664093332379 119 51 -11.666093423716083 119 52 -10.507077121032403 119 53 -11.351469161578013 119 54 -15.437983841986506 119 55 -11.334618531449502 119 56 -10.06447907329258 120 1 -1.104283073600648 120 2 -0.5819538992615136 120 3 -0.6985659384287501 120 4 -0.43268825250806486 120 5 -0.0903466974857352 120 6 0.25759005600001217 120 7 0.283383329487215 120 8 0.2650674650426197 120 9 0.29253621393373663 120 10 -0.4891228264527143 120 11 -4.605747433889377 120 12 -11.125641168923288 120 13 -11.992999754810818 120 14 -16.534010600833422 120 15 -13.53879608261176 120 16 -16.190229581570325 120 17 -15.218593874768121 120 18 -16.7496256521475 120 19 -8.493043807992755 120 20 -14.727245137676924 120 21 -5.445764441743478 120 22 -3.6047878980740222 120 23 -3.124012817105225 120 24 -8.6532162035349 120 25 -3.5175045616263176 120 26 -0.019016185101449423 120 27 -0.03519999049368039 120 28 0.5018768996783436 120 29 0.22549807229696484 120 30 0.4880175560336397 120 31 -0.5715685517821925 120 32 -10.57924358234185 120 33 -0.5343558527131336 120 34 -1.4579432298832913 120 35 -0.9159069571907139 120 36 -0.1672124111664175 120 37 0.18190228049827084 120 38 -0.4912628269312087 120 39 -0.07521719449669106 120 40 2.238438238002111 120 41 -2.891002007503669 120 42 -7.110149227462902 120 43 -13.003249279267376 120 44 -10.360301819968534 120 45 -10.287345003844887 120 46 -16.955436099007386 120 47 -15.981172899907612 120 48 -14.060281415953794 120 49 -17.668767031256067 120 50 -13.216337562720135 120 51 -11.51416598607436 120 52 -11.360251724207007 120 53 -10.380380726203864 120 54 -14.392632368063603 120 55 -11.36976592373975 120 56 -8.850193359006866 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 443.0 5 288.0 6 171.0 7 33.0 8 151.0 9 181.0 10 125.0 11 103.0 12 87.0 13 109.0 14 112.0 15 151.0 16 186.0 17 219.0 18 278.0 19 378.0 20 434.0 21 522.0 22 701.0 23 833.0 24 937.0 25 1105.0 26 1230.0 27 1361.0 28 1602.0 29 1829.0 30 2235.0 31 2506.0 32 2901.0 33 3320.0 34 3785.0 35 4622.0 36 5722.0 37 7101.0 38 6584.0 39 3754.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.45644425434844 0.4348445965212432 0.0855431993156544 0.0231679498146564 8 99.07328200741374 0.7770173937838609 0.0926717992586256 0.05702879954376961 9 90.96984602224123 6.0058454519532365 1.8694753350441973 1.1548331907613345 10 32.73274878813801 47.65290846877674 10.561020815511833 9.053321927573425 11 31.65633019674936 33.090960935272314 18.42564870259481 16.82706016538352 12 35.90141146278871 18.441688052466496 21.312731679498146 24.34416880524665 13 23.889988057679627 20.1896511772989 24.875675097588363 31.044685667433114 14 23.699030510407756 24.81465640148275 26.14592244083262 25.340390647276873 15 26.721920548728207 22.45820234352672 26.0181480422978 24.80172906544727 16 27.877739536708457 25.288442677033363 23.747704049788684 23.086113736469496 17 26.611063587111488 27.773025377815795 21.877673224978615 23.7382378100941 18 24.76653835186769 29.597946963216426 21.820644425434846 23.81487025948104 19 23.969389393317755 32.06264828127509 22.057118393121534 21.91084393228563 20 26.370431152892486 29.52425123530566 22.649351576018123 21.455966035783728 21 24.94831765041346 26.821357285429144 24.983960650128314 23.246364414029085 22 24.98039635015683 27.12610493299116 25.709295694325636 22.184203022526376 23 26.22790134017679 26.575420587396636 26.78036783575706 20.416310236669517 24 24.752722282618382 27.68441125625991 25.666981518775284 21.89588494234642 25 24.31117447870255 25.899126715380504 28.03956513990376 21.750133666013188 26 20.792785085374113 25.817203151178127 33.29590418137098 20.094107582076784 27 20.722745433789953 23.21275684931507 34.0789098173516 21.98558789954338 28 19.80448820864105 24.487138321024656 36.0037817974241 19.704591672910198 29 20.453937632606486 23.36013693012641 38.10508674024284 18.080838697024266 30 17.452989929596292 21.960609571339454 41.72890116745388 18.857499331610374 31 17.17677676963939 21.984349097131854 43.950872564573345 16.888001568655415 32 19.01644607755699 20.170525489636475 45.669080660697084 15.14394777210945 33 17.43561370004819 20.434060932731263 48.981777293901374 13.148548073319175 34 14.763305696710349 21.002050459124543 51.71971115271463 12.514932691450479 35 14.15148165317548 23.057447491352566 51.03947509182327 11.751595763648682 36 14.81336233020785 22.974081072408996 49.76291489892688 12.449641698456274 37 15.924795114896353 22.24007713321549 49.49916974663881 12.335958005249344 38 15.733609706413166 22.178939656558796 50.15992709468756 11.927523542340476 39 16.323652785463114 20.798600574763938 50.16332577691305 12.714420862859896 40 12.003946896304269 16.368855400071762 44.87979906709724 26.747398636526732 41 9.821717407713598 16.42823766219762 41.52259084863952 32.22745408144927 42 14.323702104117217 22.01381328859779 49.877750611246945 13.784733996038048 43 12.345039476974742 18.416633918045086 49.31049265835447 19.9278339466257 44 13.34013678518996 18.65399076162853 47.76560317971855 20.240269273462957 45 15.352467360825846 21.014091550427747 49.69191477201693 13.941526316729474 46 14.222206352323653 22.02245987395333 50.46330185142204 13.292031922300977 47 13.021696885418338 21.12816672906527 50.17025904334028 15.679877342176107 48 14.711289602855867 22.37215528781794 50.315037929495766 12.601517179830433 49 12.817390681517029 18.47580847330158 43.754252766536545 24.952548078644845 50 12.496431120628122 23.304782298358315 49.857244825124916 14.34154175588865 51 14.10778742718273 23.92695043064937 48.70805189235553 13.257210249812374 52 13.340113115309817 23.46875055754786 47.93305857374797 15.25807775339435 53 13.229780396357793 24.17068380646313 48.5288341367613 14.070701660417784 54 11.78898099185365 23.535086465628126 46.314491925110765 18.36144061740746 55 13.980742090501455 24.44396805830966 47.3319399038891 14.243349947299782 56 13.812993321190042 25.89021036465588 46.328440301439336 13.96835601271474 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 4.0 5 8.0 6 39.0 7 70.0 8 175.5 9 281.0 10 404.0 11 527.0 12 538.5 13 525.0 14 500.0 15 559.5 16 619.0 17 804.5 18 990.0 19 1075.5 20 1161.0 21 1321.0 22 1860.0 23 2239.0 24 2673.0 25 3107.0 26 3326.5 27 3546.0 28 3636.5 29 3613.5 30 3500.0 31 3117.0 32 2734.0 33 2352.5 34 1971.0 35 1674.5 36 1378.0 37 1241.5 38 1042.5 39 980.0 40 969.5 41 959.0 42 1005.5 43 1052.0 44 1047.5 45 1043.0 46 1109.0 47 1248.5 48 1322.0 49 1446.0 50 1570.0 51 1630.0 52 1690.0 53 1792.5 54 2029.5 55 2164.0 56 2137.5 57 2111.0 58 2147.0 59 2183.0 60 2175.5 61 2168.0 62 2014.0 63 1610.5 64 1361.0 65 1182.0 66 1003.0 67 846.0 68 689.0 69 593.0 70 497.0 71 419.5 72 290.5 73 239.0 74 204.0 75 169.0 76 121.0 77 73.0 78 62.0 79 31.0 80 11.0 81 8.5 82 6.0 83 5.0 84 4.0 85 2.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0160393498716852 14 0.0 15 0.22811519817507844 16 0.062375249500998 17 0.0 18 0.0 19 0.0944539492443684 20 0.0944539492443684 21 0.0 22 0.0 23 0.0 24 0.0017821499857428003 25 0.0035642999714856007 26 0.0106928999144568 27 0.0855431993156544 28 0.0962360992301112 29 0.044553749643570006 30 0.012475049900199599 31 0.0231679498146564 32 0.08910749928714001 33 0.1479184488166524 34 0.0481180496150556 35 0.0463358996293128 36 0.024950099800399198 37 0.18712574850299402 38 0.2655403478756772 39 0.15861134873110921 40 0.6629597946963216 41 0.8376104932991161 42 0.1407898488736812 43 0.23167949814656402 44 0.45979469632164244 45 0.21564014827487882 46 0.1799971485600228 47 0.037425149700598806 48 0.1550470487596236 49 0.47405189620758487 50 0.1283147989734816 51 0.26732249786142004 52 0.1122754491017964 53 0.1817792985457656 54 0.24237239806102082 55 0.240590248075278 56 0.20316509837467922 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 56112.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.49386940404904 #Duplication Level Percentage of deduplicated Percentage of total 1 86.43062200956938 64.38551468491588 2 7.818181818181818 11.648132306814942 3 2.433014354066986 5.437339606501284 4 1.1435406698564594 3.4074707727402336 5 0.6411483253588517 2.388080980895352 6 0.4330143540669857 1.935414884516681 7 0.26555023923444976 1.3847305389221556 8 0.19138755980861244 1.140575990875392 9 0.11244019138755981 0.7538494439692045 >10 0.5047846889952152 5.825848303393213 >50 0.01674641148325359 0.8340461933276304 >100 0.009569377990430622 0.8589962931280297 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAACGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 149 0.2655403478756772 No Hit AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 114 0.20316509837467922 No Hit AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 111 0.1978186484174508 No Hit AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 108 0.1924721984602224 No Hit AAAACGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCCAGGCTCACAACCAAA 87 0.1550470487596236 No Hit AAAACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 78 0.1390076988879384 No Hit AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 76 0.1354433989164528 No Hit AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 60 0.10692899914456801 No Hit AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA 58 0.1033646991730824 No Hit AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 58 0.1033646991730824 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0017821499857428001 0.0 0.0 0.0 0.0 9 0.0053464499572284 0.0 0.0 0.0 0.0 10 0.017821499857428 0.0 0.0 0.0 0.0 11 0.0374251497005988 0.0 0.0 0.0 0.0 12 0.0427715996578272 0.0 0.0 0.0 0.0 13 0.0463358996293128 0.0 0.0 0.0 0.0 14 0.057028799543769604 0.0 0.0 0.0 0.0 15 0.06593954947248361 0.0 0.0 0.0 0.0 16 0.0695038494439692 0.0 0.0 0.0 0.0 17 0.0819788993441688 0.0 0.0 0.0 0.0 18 0.0908896492728828 0.0 0.0 0.0 0.0 19 0.1033646991730824 0.0 0.0 0.0 0.0 20 0.1104932991160536 0.0 0.0 0.0 0.0 21 0.11405759908753921 0.0 0.0 0.0 0.0 22 0.124750499001996 0.0 0.0 0.0 0.0 23 0.1407898488736812 0.0 0.0 0.0 0.0 24 0.151482748788138 0.0 0.0 0.0 0.0 25 0.16217564870259482 0.0 0.0 0.0 0.0 26 0.169304248645566 0.0 0.0 0.0 0.0 27 0.17821499857428 0.0 0.0 0.0 0.0 28 0.1835614485315084 0.0 0.0 0.0 0.0 29 0.1924721984602224 0.0 0.0 0.0 0.0 30 0.1942543484459652 0.0 0.0 0.0 0.0 31 0.1996007984031936 0.0 0.0 0.0 0.0 32 0.20316509837467922 0.0 0.0 0.0 0.0 33 0.20494724836042202 0.0 0.0 0.0 0.0 34 0.20494724836042202 0.0 0.0 0.0 0.0 35 0.20494724836042202 0.0 0.0 0.0 0.0 36 0.2067293983461648 0.0 0.0 0.0 0.0 37 0.21385799828913601 0.0 0.0 0.0 0.0 38 0.21385799828913601 0.0 0.0 0.0 0.0 39 0.21385799828913601 0.0 0.0 0.0 0.0 40 0.21564014827487882 0.0 0.0 0.0 0.0 41 0.21742229826062162 0.0 0.0 0.0 0.0 42 0.21742229826062162 0.0 0.0 0.0 0.0 43 0.22276874821785 0.0 0.0 0.0 0.0 44 0.2263330481893356 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGATG 20 4.0238537E-4 50.311993 36 AGCGTAG 15 0.008185279 50.311993 36 TCGTGAT 20 4.04182E-4 50.266666 35 CAGCGTA 15 0.00821453 50.266666 35 ACCCGGC 15 0.008243857 50.22142 43 CGACTCC 15 0.008243857 50.22142 44 CTCGTGA 20 4.0961022E-4 50.131176 34 TGGTAGG 20 4.1143244E-4 50.086174 50 TGTCTCG 20 4.1326112E-4 50.041256 31 GTCTTGC 20 4.1326112E-4 50.041256 15 AGATCGT 15 0.00836195 50.041256 15 TTTGTCT 20 4.150962E-4 49.996414 29 GGTATCG 20 4.169378E-4 49.951656 16 AAGAGTG 25 2.0846861E-5 49.90698 10 AACCCCC 20 4.1878584E-4 49.90698 10 ATCCAGA 15 0.008451342 49.906975 10 AGGCATT 15 0.008451342 49.906975 10 AACAGTG 15 0.008451342 49.906975 10 AAGCCTT 15 0.008451342 49.906975 10 AAGCATT 15 0.008451342 49.906975 10 >>END_MODULE