FastQCFastQC Report
Fri 17 Jun 2016
SRR1526219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69229
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5320.7684640829710093No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2660.38423204148550466No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1430.20656083433243294No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1330.19211602074275233No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1290.18633809530688009No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1160.16755983764029525No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1130.16322639356339108No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC810.11700299007641306No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC720.10400265784570051No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACCT150.00803369150.56304640
AACACCA150.00803369150.56304639
GTTTAAT150.008057022550.52600541
ATGCTCG402.4665496E-950.34160636
TGCTCGT402.4665496E-950.34160637
TATGCCC150.00817443650.34160235
ATATGCC252.0024361E-550.26822334
ATATGCT1350.050.2682234
TGTGGGA204.0771905E-450.19505344
CTGACCG150.00826927950.1950544
CGACTCC150.00826927950.1950544
TACCAAG150.00829311750.15854647
CAACGAT252.0285222E-550.15854646
CGTGCAA301.0111162E-650.15854648
CGATTAA150.00829311750.15854649
ACGATTC150.00829311750.15854648
AACGATT252.0285222E-550.15854647
CTACCAA150.00829311750.15854646
ACGAGCC150.00829311750.15854647
ACCGCTC204.0918734E-450.15854347