FastQCFastQC Report
Fri 17 Jun 2016
SRR1526218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4410
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT420.9523809523809524No Hit
GTCCCGGGGGGAGCTTCAGATGACATGACTGTGGTAGATGCAGCTTCATTAAGACG240.5442176870748299No Hit
GTCCCGAGGAATGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT220.4988662131519274No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT180.40816326530612246No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTCTTTTTTTTTTTTTTTT160.36281179138321995No Hit
GTCCCGGGGGGTGTGAATGTTGCCGGCGTCTCCCTGAAGTCTCTTAACCCAGAACT150.3401360544217687No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC140.31746031746031744No Hit
GTCCCGGGGAGAGACCCAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.31746031746031744No Hit
GTCCCGGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.27210884353741494No Hit
GTCCCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG120.27210884353741494No Hit
GTCCCGGGGGCTCTTGCTTGCTTTTCTCTCTCTCTTGCTTCTTGCTCTCTTTTCCT110.2494331065759637No Hit
GTCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA100.22675736961451248No Hit
GTCCCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAAAAAAAAAAAAAAAAA90.20408163265306123No Hit
GTCCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.20408163265306123No Hit
GTCCCGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTC90.20408163265306123No Hit
GTCCCGGGATATCAAGACCCAGAAACTGAAGAAAAATCTTGTTTTCTCATGCATAC90.20408163265306123No Hit
GTCCCGGGAAGCATGGTTACCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT80.18140589569160998No Hit
GTCCCGGGGATGCCGCAGATCGACTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTT70.15873015873015872No Hit
GTCCCGGGGAAGTGGTCACGCAGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT70.15873015873015872No Hit
GTCCCGAGGAATGGTCAACGCAGAGTCGACTTTTTTTTTCTTTTTTTTTTTTTTTT70.15873015873015872No Hit
GTCCCGGGCATGTATCAACGCAGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT70.15873015873015872No Hit
GTCCCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13605442176870747No Hit
GTCCCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTCGCAGCATTTGATATG60.13605442176870747No Hit
GTCCCGGGGAAGCATGGTATCAACGCAGAATCGACTTTTTTTTTTTTTTTTTTTTT60.13605442176870747No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTCCTTTTTTTTTTTTTTT60.13605442176870747No Hit
GTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCC60.13605442176870747No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC60.13605442176870747No Hit
GTCCCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCCGGCAGCATTTGATATG60.13605442176870747No Hit
GTCCCGGGGGAGTCACGCAGAGTCGATTTTTTTGTTTTTTTTTTTTTTTTTTTTTT60.13605442176870747No Hit
GTCCCGGGGAGCTGGTTCAACGCAGAGTCGCTTTTTTTTTTTTTTTTTTTTTTTTT60.13605442176870747No Hit
GTCCCGGGGCCTTTTTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGG50.11337868480725624No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC50.11337868480725624No Hit
GTCCCGGGGCTGTCCCGGGGGCATGATTCTTGCGATTCTGAATCTAAAATTATTTC50.11337868480725624No Hit
GTCCCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAACAAAAAAAAAAAAAAA50.11337868480725624No Hit
GTCCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA50.11337868480725624No Hit
GTCCCGGGGGAGGCAGAGGAGTCACCAGCCACATCAGCAGTGAAAACTTCAATCTT50.11337868480725624No Hit
GTCCCGTTACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11337868480725624No Hit
GTCCCGGGGGGTGTGAATGTTGCCGGCGTCTCCCTGAAGCCTCTTAACCCAGAACT50.11337868480725624No Hit
GTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGACTGTTCAAAGCAGGCC50.11337868480725624No Hit
GTCCCGGGGTGGATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11337868480725624No Hit
GTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGCGTGTTCAAAGCAGGCC50.11337868480725624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCAA150.00773927950.2298837
AACCAAA150.00773927950.2298838
CGGGGGG458.731149E-1149.6590965
CGGGGAG503.6379788E-1249.6590965
CGGGGAC251.8381548E-549.6590965
GCAGTGG251.8381548E-549.65909612
GGGGGCC251.8381548E-549.6590966
GGAAGCA251.8381548E-549.6590968
GTGGTAT251.8381548E-549.65909615
AAGCAGT251.8381548E-549.65909610
TGGTATC308.67989E-749.65909216
CGGGGTG150.00810337249.6590925
TTCAACG150.00810337249.65909216
CGGGGGC354.073263E-849.6590925
CGGGGCT150.00810337249.6590925
CCCGGGA354.073263E-849.6590923
CGGGGAT150.00810337249.6590925
GGGAGCT150.00810337249.6590927
GGAAGCC150.00810337249.6590928
CCGGGGT354.073263E-849.6590924
Produced by FastQC (version 0.11.5)